characteristics.text: GSC:MIxS v2.1
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2012-01-10T15:58:44Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: GSC, MIxS
characteristics.characteristic: ENA-KEYWORD
characteristics.text: 2018-03-09T09:55:25Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1094730
characteristics.characteristic: External Id
characteristics.text: Germany, Lower Saxony
characteristics.characteristic: Geographic location (country:region,area)
characteristics.text: VTI
characteristics.characteristic: INSDC center name
characteristics.text: 2012-01-10T15:58:44Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-09T09:55:25Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS084152
characteristics.characteristic: SRA accession
characteristics.text: 3BT_c
characteristics.characteristic: Submitter Id
characteristics.text: BBCH 63 - 65; August 2009
characteristics.characteristic: collection date
characteristics.text: maize culticar 3BT
characteristics.characteristic: current vegetation
characteristics.text: 2-15 cm
characteristics.unit: m
characteristics.characteristic: depth
characteristics.text: Rhizosphere microbial communities were collected by saline washes of coarse roots (diameter 2-4 mm) of maize cultivar 3BT, collected at the flowering stage BBCH 63-65. Total DNA was extracted from the collected cells by “FastDNA SPIN kit for soil” (MP Biomedicals, Illkirch, France). The extraction included two bead beating steps of 45 s at 6.5 m s-1 on a FastPrep-24 system (MP Biomedicals) and additional washing steps of the binding matrix each with 1 ml 5.5 M guanidine thiocyanate (Carl Roth GmbH) until the matrix retained its original color (1 - 3 washes).
characteristics.characteristic: description
characteristics.text: soil
characteristics.characteristic: enviornmental package
characteristics.text: field soil; ENVO:00005755
characteristics.characteristic: environment (featurel)
characteristics.text: rhizosphere; ENVO:00005801
characteristics.characteristic: environment (material)
characteristics.text: 52° N
characteristics.characteristic: geographic location (latitude)
characteristics.text: 10° E
characteristics.characteristic: geographic location (longitude)
characteristics.text: MIMARKS
characteristics.characteristic: investigation type
characteristics.text: “FastDNA SPIN kit for soil” (MP Biomedicals, Illkirch, France)
characteristics.characteristic: nucleic acid extraction
characteristics.text: rhizosphere metagenome
characteristics.characteristic: organism
characteristics.text: initial denaturation: 95degC_15min; 30 cycles of: denaturation: 94degC_60s, annealing: 50degC_60s, extention: 72degC_70s; final extension at 72degC_5min
characteristics.characteristic: pcr conditions
characteristics.text: F_Com1_MID11: ccatctcatccctgcgtgtctccgactcagTGATACGTCTCAGCAGCCGCGGTAATAC; R_Com2: cctatcccctgtgtgccttggcagtctcagCCGTCAATTCCTTTGAGTTT
characteristics.characteristic: pcr primers
characteristics.text: 6.1
characteristics.characteristic: ph
characteristics.text: maize rhizosphere
characteristics.characteristic: project name
characteristics.text: rhizosphere metagenome
characteristics.characteristic: scientific_name
characteristics.text: Pyrosequencing
characteristics.characteristic: sequencing method
characteristics.text: 16S rRNA
characteristics.characteristic: target gene
characteristics.text: Rhizosphere microbial communities were collected by saline washes of coarse roots (diameter 2-4 mm) of maize cultivar 3BT, collected at the flowering stage BBCH 63-65. Total DNA was extracted from the collected cells by “FastDNA SPIN kit for soil” (MP Biomedicals, Illkirch, France). The extraction included two bead beating steps of 45 s at 6.5 m s-1 on a FastPrep-24 system (MP Biomedicals) and additional washing steps of the binding matrix each with 1 ml 5.5 M guanidine thiocyanate (Carl Roth GmbH) until the matrix retained its original color (1 - 3 washes).
characteristics.characteristic: title