characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-11-11T17:02:10Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T20:51:07Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3112879
characteristics.characteristic: External Id
characteristics.text: MDC
characteristics.characteristic: INSDC center name
characteristics.text: 2014-11-11T17:02:10Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T20:51:07Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS580100
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2572:raw_rbm20_6sg_NH_HM_04
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HEK-293
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001182
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Human embryonic kidney (HEK) 293 cells with tetracycline-inducible expression of FLAG/HA-tagged RBM20 have been described previously (5). For PAR-CLIP, we essentially followed the protocol as described (13). The libraries were sequenced on an Illumina Genome Analyzer GAII using the 50 bp single read protocol. 4 ug of total RNA was used for library preparation with the TruSeq RNA Sample Preparation Kit (Illumina).
characteristics.characteristic: description
characteristics.text: PAR-CLIP 3
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: raw_rbm20_6sg_NH_HM_04
characteristics.characteristic: title