characteristics.text: 2012-07-20T17:00:48Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:39:07Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SRA-Webin
characteristics.characteristic: ENA-SUBMISSION-TOOL
characteristics.text: SAMEA1466837
characteristics.characteristic: External Id
characteristics.text: IPK-Gatersleben
characteristics.characteristic: INSDC center name
characteristics.text: 2012-07-20T17:00:48Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:39:07Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS102963
characteristics.characteristic: SRA accession
characteristics.text: barley ETCs
characteristics.characteristic: Submitter Id
characteristics.text: domesticated barley
characteristics.characteristic: common name
characteristics.text: Differentiating barley ETCs (3 DAF, 5 DAF, 7 DAF) have been isolated from cryosections of barley grains using laser-assisted microdissection. Total RNA was extracted from tissue sections and mRNA was amplified by one round of T7-based amplification. The three antisense (a)RNA populations were pooled in equal amounts and first strand cDNA synthesis was primed with a N6 randomized primer. After ligation of 454 adapters and PCR-amplification (20 cycles), cDNA was normalized and fractioned cDNA in the size range of 400 to 600 nucleotides (gel fractioning) was used for GS FLX Titanium sequencing.
characteristics.characteristic: description
characteristics.text: Hordeum vulgare subsp. vulgare
characteristics.characteristic: organism
characteristics.text: Hordeum vulgare subsp. vulgare
characteristics.characteristic: scientific_name
characteristics.text: Pool of differentiating barley endosperm transfer cells from grains (3 DAF, 5 DAF, 7 DAF).
characteristics.characteristic: title