characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-12-31T17:01:14Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T21:48:55Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3139809
characteristics.characteristic: External Id
characteristics.text: Institute for molecular and tumor biology Philipps University Marburg Germany
characteristics.characteristic: INSDC center name
characteristics.text: 2015-12-31T17:01:14Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T21:48:55Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS611773
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3113:TA070
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: macrophage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000235
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were isolated on October 14th 2014 from two samples of healthy human donor blood of unknown gender via LSM1077 gradient centrifugation. Adherent cells were positivly selected on primaria 6 well dishes (3x10^6 monocytes per well in 2 ml PBS (PAA)) followed by 6 washing steps with PBS. Finally the monocytes were cultured for 7d in humidified incubator at 37 °C, 5 % CO2 in 2ml RPMI medium supplemented with 10 % FCS (both PAA). Lysis in hypotonic lysis buffer Nuclei were resuspended in ChIP RIPA and sheared with a Branson S250D using a microtip (60 pulses of 1 s, 2 s pause, 20% amplitude) with coolling (ice-ethanol). A 15 min 20,000xg supernatant was used for IP (after preclearing). The Diagenode Microplex Library Preparation kit (Diagenode, C05010011) was used according to the manufacturer's instructions with 1-2 ng of input DNA. DNA concentrations were measured on a Nanodrop 3300 using the Quant-It dsDNA High-Sensitivity Assay Kit (Life Technologies).
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: TA070
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005309
characteristics.characteristic: title