characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-03-19T17:02:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:41:51Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2177680
characteristics.characteristic: External Id
characteristics.text: University of Manchester
characteristics.characteristic: INSDC center name
characteristics.text: 2018-03-19T17:02:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:41:51Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS345774
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1851:Nd-INPUT-S2-B
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HeLa
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001185
characteristics.characteristic: cell line
characteristics.text: epitheloid cervix carcinoma cell
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: The preparation of the material was as described in Elsby et al., 2009. HeLa cells were crosslinked and frozen in order to pool enough for ChIP. Elsby,L.M., Donn,R., Alourfi,Z., Green,L.M., Beaulieu,E. and Ray, D.W. (2009) Hypoxia and glucocorticoid signaling converge to regulate macrophage migration inhibitory factor gene expression. Arthritis Rheum., 60, 2220-2231. ChIP assays were performed as described in Elsby et al., 2009, with the following modifications for ChIP-seq. The crosslinked material was sheared by twice probe sonication (SONICS VCX130) with energy more than 50% as 12 secs on/ 40secs off, followed by water sonication using bio-rupter (Next Generation) for 40 mins, at high level, 30 secs on/ 30 secs off, 4 degrees C. The size of sheared DNA fragments was between 100 bp to 500 bp, with most was around 200 bp. Immunoprecipitation was performed by incubating 1x10^7cells with 3 ug of GR antibodies (1ug anti-GR M-20 and 1 ug anti-GR H-300X from Santa Cruz Biotechnology, and 1 ug anti-GR from Sigma-Aldrich), overnight at 4 degrees C, followed by protein G dynabeads (Invitrogen). Elsby,L.M., Donn,R., Alourfi,Z., Green,L.M., Beaulieu,E. and Ray, D.W. (2009) Hypoxia and glucocorticoid signaling converge to regulate macrophage migration inhibitory factor gene expression. Arthritis Rheum., 60, 2220-2231.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Nd-INPUT-S2-B
characteristics.characteristic: title