characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-12-19T17:02:55Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T22:02:04Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3177773
characteristics.characteristic: External Id
characteristics.text: Linkoping University, Linkoping, Sewden
characteristics.characteristic: INSDC center name
characteristics.text: 2014-12-19T17:02:55Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T22:02:04Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS630705
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3183:MEF_CULTURE_IP
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: mouse embryonic fibroblast cell
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004040
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: hmeDIP-Seq was performed as previously described. Briefly, DNA was sonicated to yield fragmented DNA with a modal size of 150 bp. Following end-repair and A-tailing, Illumina TruSeq adaptors were added to the resulting DNA according to the manufacturer’s instructions. The adaptor ligated DNA was then subjected to hmeDIP as outlined in associated manuscript. The resulting INPUT and hMeDIP samples were PCR amplified for fifteen cycles using single-end Illumina PCR primers. Finally, libraries were size selected by agarose gel electrophoresis. All DNA purification steps were carried out using Agencourt AMPure Beads (Beckman Coulter), and DNA quality, quantity and size was tested after every step on an Agilent Bioanalyser using High Sensitivity DNA chips (Agilent). Samples were subjected to single-end sequencing on the Illumina GAIIx platform. Sequencing was carried out at the Genomics Core, Albert Einstein College of Medicine, New York.
characteristics.characteristic: description
characteristics.text: cultured cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000001
characteristics.characteristic: growth condition
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: MEF_CULTURE_IP
characteristics.characteristic: title