characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-07-16T15:54:27Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:18:51Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2422481
characteristics.characteristic: External Id
characteristics.text: Centre for Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, United Kingdom
characteristics.characteristic: INSDC center name
characteristics.text: 2015-07-16T15:54:27Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:18:51Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS427380
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2349:growing_U2OS_cells_6
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: U2OS
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002869
characteristics.characteristic: cell line
characteristics.text: human osteosarcoma cell
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Human osteosarcoma (U2OS) cells were obtained from CRUK and grown at 37C according to standard cell culture protocols. U2OS cells were grown at 37C and either left untreated or incubated at 43C for 4 hours to induce heat shock. Qiagen RNeasy mini kit according to manufacturer's instructions including DNAseI digestion step Isolated total RNA was incubated with DNaseI to digest residual genomic DNA. Poly-A mRNA was purified from the total RNA sample using oligo(dT) coated magnetic beads and eluted with Tris-HCl and heating. Isolated mRNA was fragmented and used for reverse transcription of cDNA (first and second strand cDNA synthesis). Purified cDNA was subjected to end-repair and A-tailing followed by adaptor ligation. Adaptor-ligated cDNA fragments were then PCR amplified and quality and quantity of the cDNA library was validated on an Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-time PCR system, respectively.
characteristics.characteristic: description
characteristics.text: 43-4
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: growing_U2OS_cells_6
characteristics.characteristic: title