characteristics.text: 2013-11-30T17:01:21Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:17:26Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2052646
characteristics.characteristic: External Id
characteristics.text: Humanitas Clinical and Research Center
characteristics.characteristic: INSDC center name
characteristics.text: 2013-11-30T17:01:21Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:17:26Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS215416
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1507:RNA_sham_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: cardiac myocyte
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000746
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: 2-month-old male C57BL/6J mice were subjected under anesthesia to transverse aortic constriction for 1 week. Cardiomyocytes were isolated from the hearts at the end of perfusion with collagenase (collagenase type II). A pool of 8 libraries was made using 473 pg of total DNA. In each case, the DNA was added to the PCR mix containing in a final volume of 5.6 mL: PCR Gold Buffer (Applied Biosystems), 1 ; AmpliTaq Gold, 3000 U; ePCR primer 1, 40 nM; ePCR primer 2, 3 uM; deoxynucleotide mix, 3.5 mM each; MgCl2, 25 mM; 1.6 billion SOLiD sequencing beads (Applied Biosystems). The PCR mix was added to 50 mL SOLiD ePCR Tube containing 9 ml of oil phase and emulsified using Ultraturrax Tube Drive (IKA). The emulsion was dispensed into 96-well plate and amplified for 40 cycles in a 9700 PCR device (Applied Biosystems). After amplification, the emulsion was broken disrupted with butanol, beads were enriched for template positive beads, 3p-end extended and covalently attached onto sequencing slides. A pool of eight samples were deposited on one sequencing slide and sequenced using standard settings on the SOLiD system version 3.5 to produce 50 nucleotide long reads.
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain or line
characteristics.text: RNA_sham_1
characteristics.characteristic: title