characteristics.text: 2011-03-15T17:00:17Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-09T09:53:17Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1027589
characteristics.characteristic: External Id
characteristics.text: EASIH
characteristics.characteristic: INSDC center name
characteristics.text: 2011-03-15T17:00:17Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-09T09:53:17Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS025256
characteristics.characteristic: SRA accession
characteristics.text: GS115
characteristics.characteristic: StrainOrLine
characteristics.text: E-MTAB-562:HuLy_SS_SORB
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Derivatives of Pichia pastoris strain GS115 were grown to steady state in chemostat cultures (1L volume in 2L fermenter; 30oC; pH5; 750 rpm stirring; air flow 1L/min) in a defined carbon-limited medium based on sorbitol (20 g/L). Dilution rates at steady state were 0.004-0.015 L/h, and all other non-carbon nutritional requirements were in excess at constant residual concentrations. Cells were lysed mechanically in the presence of trizol using a mikrodismembranator. After partitioning with chloroform, RNA was precipitated from the aqueous phase using isopropanol. RNA was washed with 70% ethanol, redissolved in water then subjected to a lithium chloride precipitation. 10 microg aliquots of the final RNA solution were depleted for rRNA using the Ribominus Eukaryote kit for RNA seq (Invitrogen), and the rRNA-depleted sample used for sequencing library construction.
characteristics.characteristic: description
characteristics.text: pPIC9-HuLy
characteristics.characteristic: genotype
characteristics.text: Komagataella pastoris
characteristics.characteristic: organism
characteristics.text: Pichia pastoris
characteristics.characteristic: organism
characteristics.text: Komagataella pastoris
characteristics.characteristic: scientific_name
characteristics.text: HuLy_SS_SORB
characteristics.characteristic: title