characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-06-17T17:01:16Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T21:28:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2747173
characteristics.characteristic: External Id
characteristics.text: INRA, UMR1313 Genetique Animale et Biologie Integrative
characteristics.characteristic: INSDC center name
characteristics.text: 2015-06-17T17:01:16Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T21:28:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS539981
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2755:Bovine Lactation MG TDM Pool 3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: cattle
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/LBO_0001036
characteristics.characteristic: common name
characteristics.text: biopsies Protocols: Total RNA was extracted from a 50 mg specimen using 750 ul Trizol LS reagent (Invitrogen, Cat. N. 10296-010), treated with DNase I (QIAGEN, Cat. N. 79254) and then purified using NucleoSpin RNA Clean-up (Macherey-Nagel, Cat. N. 740948.50). Total RNA was eluted with 50 ul DNase I-free water. Approximately 20 to 30 ug were recovered per biopsy. RiboMinus RNA (ribosomal RNA-depleted RNA) was purified from 5 ug total RNA using the RiboMinus Eukaryote Kit for RNA-Seq (Invitrogen, Cat. N. A10837-08). RNA Integrity was assessed using an Agilent Bioanalyzer. Strand orientated libraries were prepared with the Truseq Small RNA sample prep Kit (Illumina), the final gel purification step being replaced by a PCR cleanup with AMPureXP beads (Beckman-Coulter).
characteristics.characteristic: description
characteristics.text: Lactation day 53-74
characteristics.characteristic: developmental stage
characteristics.text: Bos taurus
characteristics.characteristic: organism
characteristics.text: Bos taurus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9913
characteristics.characteristic: organism
characteristics.text: mammary gland
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0000817
characteristics.characteristic: organism part
characteristics.text: Bos taurus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Bovine Lactation MG TDM Pool 3
characteristics.characteristic: title