characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-10-21T17:04:38Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:43:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2177955
characteristics.characteristic: External Id
characteristics.text: CRUK
characteristics.characteristic: INSDC center name
characteristics.text: 2014-10-21T17:04:38Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:43:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS346067
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1865:LY2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: LY2
characteristics.characteristic: cell line
characteristics.text: epithelial cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000066
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: MCF-7 cells [American Type Culture Collection (ATCC)] were grown in DMEM with 2mM L-glutamine, 10% fetal bovine serum, 50U/ml penicillin and 50ug/ml streptomycin in 37C incubator with 5% CO2. LY2 cells (kind gift from R. Clarke, Georgetown, Washington D.C.) were grown in phenol red-free MEM with 2mM L-glutamine, 10% charcoal dextrin stripped fetal bovine serum, 50U/ml penicillin and 50ug/ml streptomycin in 37C incubator with 5% CO2. Cells were steroid depleted for 3 days prior to treatment with Tamoxifen for 45 minutes. Proteins were cross linked to DNA by treating with formaldehyde (1%) for 10 minutes (ER) or 60 minutes (HMGB2). Chromatin immunoprecipitation and preparation of libraries was carried out as described in Schmidt et al (2009) Methods 48:240-248.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: LY2
characteristics.characteristic: title