characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-05-29T16:09:48Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T20:48:39Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2821501
characteristics.characteristic: External Id
characteristics.text: Genome organization and Function German Cancer Research Center (DKFZ)
characteristics.characteristic: INSDC center name
characteristics.text: 2015-05-29T16:09:48Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T20:48:39Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS568752
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3020:Poly(A)+ RNA 1 - 0 min
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HUVEC cell line
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002795
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Cell were treated with the pro-inflammatory cytokine TNFa for 0 or 30 min. Short and long RNA fractions were separated and DNase-treated after cell fractionation starting with about 1.5x10e7 cells using the miRNeasy mini kit (qiagen). RNA fraction were transformed into cDNA library with solexa adaptors using the standard Illumina protocol. Libraries were prepared using the Nugen multiplex library prep kit.
characteristics.characteristic: description
characteristics.text: Poly(A)+ RNA 1 - 0 min
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Poly(A)+ RNA 1 - 0 min
characteristics.characteristic: title