characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-09-14T17:01:06Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T20:49:32Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3110451
characteristics.characteristic: External Id
characteristics.text: Laboratory of Genetics - Gage The Salk Institute for Biological Studies
characteristics.characteristic: INSDC center name
characteristics.text: 2015-09-14T17:01:06Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T20:49:32Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS577667
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3037:Sample 80
characteristics.characteristic: Submitter Id
characteristics.text: 49 year
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000036
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Human post-mortem prefrontal cortex
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: X49yr_Ag8_PFC Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 80
characteristics.characteristic: title