characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-05-21T14:57:04Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T19:53:01Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2639694
characteristics.characteristic: External Id
characteristics.text: EBI
characteristics.characteristic: INSDC center name
characteristics.text: 2015-05-21T14:57:04Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T19:53:01Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS505778
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2733:Dmelanogaster_FDFSS
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: D. melanogaster mixed sex adult RNA sample. Small RNA libraries were generated by Illumina TruSeq v2 after FDFSS treatment Protocols: Total RNA was extracted from N. benthamiana tissue based on the White and Kaper protocol (/White, J.L., and Kaper, J.M. (1989). A simple method for detection of viral satellite RNAs in small plant tissue samples. J. Virol. Methods 23, 83 to 93). Total RNA from the all other organisms (A. thaliana, M. musculus, C. elegans, D. melanogaster) were extracted by TRIzol reagent (Life Technolgies). To make the small RNA libraries, this total RNA was used directly for Illumina TruSeq small RNA cloning protocol (RS-200-9002DOC) (NSS= non size selected). Or was subject to this protocol after small RNA size selection, by extraction of the 18-30nt band from a denaturing (formamide) polyacrylamide gel (FSS) or after small RNA size selection by extraction of the 18-30nt band from a fully denaturing formaldehyde polyacrylamide gel (FDFSS - described in detail by Harris et al,. in prep).
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organism part
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: Oregon-R
characteristics.characteristic: strain
characteristics.text: Dmelanogaster_FDFSS
characteristics.characteristic: title