characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2021-02-19T04:08:52Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T19:14:35Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2700090
characteristics.characteristic: External Id
characteristics.text: Vanderbilt University
characteristics.characteristic: INSDC center name
characteristics.text: 2021-02-19T04:08:52Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T19:14:35Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS527824
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2816:E8.0 Sox17^GFP/+ embryos 1
characteristics.characteristic: Submitter Id
characteristics.text: embryonic day 8
characteristics.characteristic: age measurement datum
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: pool of 6 E8.0 (5~7 somites) Sox17^GFP/+ embryos Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Embryos were genotyped by fluorescence microscopy. The embryos were dissected and the dissection event was confirmed by checking the marker protein (e.g., GFP) fluorescence. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II.
characteristics.characteristic: description
characteristics.text: embryo stage
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007725
characteristics.characteristic: life cycle stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: STOCK Sox17tm2(EGFP/cre)Mgn/Mmnc mouse strain
characteristics.characteristic: strain
characteristics.text: E8.0 Sox17^GFP/+ embryos 1
characteristics.characteristic: title