characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-01-10T17:02:31Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T19:24:11Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2699501
characteristics.characteristic: External Id
characteristics.text: College of Forensic Medicine, Xian Jiaotong University Health Science Center
characteristics.characteristic: INSDC center name
characteristics.text: 2016-01-10T17:02:31Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T19:24:11Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS527235
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2843:METH
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mice were given once daily injections of saline for two consecutive days (day 1-2), after which they were divided into two groups randomly. Two groups of mice were then given once daily injections of methamphetamine (METH, 2mg/kg) or saline for five consecutive days (day 3-7) followed by two injection-free days (day 8-9). On day 10, mice were given a challenge injection of either 2 mg/kg dose of METH or saline. Horizontal locomotor activity was performed on all drug treatment days for 60 minutes before and after the injections. For all experiments, mice were sacrificed 24 h after the last injection. After mice were sacrificed, nucleus accumbens (NAc) were harvested and NAc lysate from eight mice of each group were pooled as one sample for total RNA isolation, following to the manufacturers instructions of TRIzol (Invitrogen, USA). The RNA quality was evaluated by Agilent 2100 BioAnalyzer (Agilent Technologies, USA), and all samples exhibited an RIN > 8. Total RNA (5ug) of each sample was fragmented into ~200 base pair (bp) using a Covaris-S2 system after removed rRNA without preselecting mRNA. The first cDNA strand was synthesized using random hexamers and second strand cDNA was synthesized where dUTP was used instead of dTTP by DNA polymerase I. Then the double stranded cDNA were end-repaired after purified by QiaQuick PCR kit, and adapters were ligated. Subsequently, the uridine-containing strand was destructed by Uracil-N-Glycosylase, which enabled the identification of transcript orientation. After that, for acquiring the sequencing library products, the single stranded adapted cDNA fragments of 200 bp were recovered and purified with agarose gel electrophoresis and then enriched by PCR for 12 cycles.
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: nucleus accumbens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001882
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: METH
characteristics.characteristic: title