characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2013-11-16T17:01:09Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:52:06Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2225929
characteristics.characteristic: External Id
characteristics.text: Uppsala University, Uppsala, Sweden and Broad Institute, Boston, MA
characteristics.characteristic: INSDC center name
characteristics.text: 2013-11-16T17:01:09Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:52:06Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS362632
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1984:GREY_BI_011_COMSTOCK_11
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: dog
characteristics.characteristic: common name
characteristics.text: Protocols: Genomic DNA was prepared from whole blood QIAamp DNA Midi kit For each sample, 3 ug of genomic dna was sonicated to 200-800 bp and library preparation was performed using the Illumina paired end kit. Fragments were blunt ended, a 3' adonesine overhang was added followed by ligation of adapters. Ligated fragments were enriched using 11-15 cycles of adapter specific PCR using a high-fidelity polymerase (Phusion, Finnzymes).1 ug of cleaned PCR product was hybridized on the Roche Nimblegen Sequence Capture array with 385,000 probes. The array was customized to target 95% of the 5 Mb target region. Enriched DNA samples were sequenced using paired-end sequencing.
characteristics.characteristic: description
characteristics.text: osteosarcoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000637
characteristics.characteristic: disease
characteristics.text: BI_011
characteristics.characteristic: individual
characteristics.text: Canis lupus familiaris
characteristics.characteristic: organism
characteristics.text: Canis familiaris
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9615
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Canis lupus familiaris
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: GREY_BI_011_COMSTOCK_11
characteristics.characteristic: title