characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-10-10T17:01:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:52:21Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2228018
characteristics.characteristic: External Id
characteristics.text: University of California, Riverside
characteristics.characteristic: INSDC center name
characteristics.text: 2014-10-10T17:01:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:52:21Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS362743
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1988:cell_culture_M_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: U2OS
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002869
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: U2OS cells were maintained in McCoys 5A media (Invitrogen) 20 J/m2 UVC irradiation treatment for 0, 8 or 16 hr Formaldehyde Cross-linking and chromatin immunoprecipitation assays for this experiment were performed as described by Boyd and Farnham (MCB 29:8389-8299) and Y. Shang et al. (Cell 103:843-852) with some modifications. Formaldehyde concentration used was 1% for 10 minutes. Antibody used for immunoprecipitation was Rabbit p53 (Santa Cruz FL-393) Purified ChIP DNA was used to prepare Illumina multiplexed sequencing libraries. Libraries for Illumina sequencing were prepared following the Illumina-compatible NEXTflex ChIP-Seq Kit (Bioo Scientific).
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: cell_culture_M_1
characteristics.characteristic: title