characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-05-01T17:02:16Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:37:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2531515
characteristics.characteristic: External Id
characteristics.text: The University of Manchester
characteristics.characteristic: INSDC center name
characteristics.text: 2015-05-01T17:02:16Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:37:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS455723
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2568:SRO9-4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: baker's yeast
characteristics.characteristic: common name
characteristics.text: Protocols: Grown in SCD media at 30C to exponential phase (OD600 0.5 - 0.7), pelleted by centrifugation and snap frozen into liquid nitrogen Samples lysed in Lola lysis buffer using a 6870 Freezer Mill (Spex). Lysates cleared by centrifugating twice at 15000 g. Nucleic acids extracted using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit. Depleted samples were precipitated with 2.5x volumes ethanol, 1/10th volume 3M sodium acetate and 1_l glycogen, washed twice with 70% ethanol and re-suspended in 10_l DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. cDNA was then amplified and barcoded with SOLiD RNA barcoding Kit and samples purified using PureLink PCR Micro Kit
characteristics.characteristic: description
characteristics.text: Sro9p-TAP-tag
characteristics.characteristic: genotype
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4932
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: scientific_name
characteristics.text: Mat a
characteristics.characteristic: sex
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: BY4741
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000098
characteristics.characteristic: strain
characteristics.text: SRO9-4
characteristics.characteristic: title