characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-09-20T17:03:04Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:08:16Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2335646
characteristics.characteristic: External Id
characteristics.text: MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK
characteristics.characteristic: INSDC center name
characteristics.text: 2014-09-20T17:03:04Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:08:16Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS393840
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2237:tfs1-dis3.1_chip
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fission yeast
characteristics.characteristic: common name
characteristics.text: Protocols: S. pombe cells were grown in EMM medium supplemented with 20 ug/ml Thiamine at 30C for 10 hours (final OD600: 0.5-0.6). Cells were harvested and fixed for 20 minutes with 1% formaldehyde (Larochelle et al. NAR 2012, 40:10240) Whole cell extract from fixed cells was prepared as described in (Larochelle et al. NAR 2012, 40:10240) ChIP experiments were performed as described in (Larochelle et al. NAR 2012, 40:10240). For ChIP-seq experiments, chromatin immunoprecipitation was done as described above with the following modifications. We scaled up the reactions to obtain larger amount of chromatin for library preparation. Chromatin was immunoprecipitated with Pan mouse IgG Dynabeads (Invitrogen) antibody against Rpb1 (8WG16, Convance). The 8WG16 antibody is known to recognize the C-terminal domain (CTD) of Rpb1 and measures total RNAPII along genes in yeast. Libraries were prepared using the NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina kit (New England BioLabs) according to the manufacturer instructions.
characteristics.characteristic: description
characteristics.text: h+ his7-366 leu1-32 ura4-d18 ade6-m210 TFS1::kanMX6 p81NMT1-DIS3::natMX6
characteristics.characteristic: genotype
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4896
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: scientific_name
characteristics.text: mating_type_h_plus
characteristics.characteristic: sex
characteristics.text: FBY-1530
characteristics.characteristic: strain
characteristics.text: tfs1-dis3.1_chip
characteristics.characteristic: title