characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-02-23T17:01:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T18:21:40Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2594013
characteristics.characteristic: External Id
characteristics.text: University of Dundee
characteristics.characteristic: INSDC center name
characteristics.text: 2015-02-23T17:01:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T18:21:40Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS480258
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2651:dsRED.2-4
characteristics.characteristic: Submitter Id
characteristics.text: 4 day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Kc167
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005819
characteristics.characteristic: cell line
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: Kc167 cells were grown in Schneiders medium containing 10% FBS. Cells were transfected with dsRNAs targeting each gene using FugeneHD transfecton reagent. After 4 days, aliquot of cells were analysed by flow cytometry and remaining cells were collected for RNA extraction. Cells were lysed with QIAzol Lysis Reagent (Qiagen). After chloroform addition, total was precipitated from the aqueous phase with 1/10 volume of 3M sodium acetate, pH 5.0 and one volume of isopropanol. 15 ug total was fragmented for 3 min at 70 degreesC in fragmentation buffer (Ambion). Fragmentation was terminated by cooling of the sample on ice and addition of EDTA to 17 mM final concentration. 2.5 ul fragmented was then mixed with 0.5 ul 100 mM MgCl2 and diluted to 30 ul with RNAse-free water.
characteristics.characteristic: description
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: dsRED.2-4
characteristics.characteristic: title