characteristics.text: 2013-01-04T17:03:17Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:13:44Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1696397
characteristics.characteristic: External Id
characteristics.text: Univ. of Edinburgh, WTCCB
characteristics.characteristic: INSDC center name
characteristics.text: 2013-01-04T17:03:17Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:13:44Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS202635
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1435:56_Mnase
characteristics.characteristic: Submitter Id
characteristics.text: 18 hour
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000032
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fission yeast
characteristics.characteristic: common name
characteristics.text: Protocols: S. pombe strains were grown in complete media (YES) at 32 C to 5 x 106 cells ml1, fixed for 15 min in 1 per cent formaldehyde (Sigma) and treated with 0.4 mg ml 1 Zymolase (AMS Biotechnology Europe) in PEMS for 1 h. To fragment the DNA, 300 ul aliquots of sample was either sonicated for 20 min in a Bioruptor (Diagenode) or treated with 0.4 U miccrococcal nuclease (MNase; Sigma) at 37 C for 6 min in MNase digestion buffer (50 mM Hepes pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 1x Proteinase inhibitor mix (Sigma) and 1 mM phenylmethylsulfonyl fluoride (PMSF)). The MNase reaction was stopped by adding 300 ul lysis buffer 2 (50 mM Hepes pH 7.5, 255 mM NaCl, 12 mM EDTA, 2% Triton X-100, 0.2% sodium deoxycholate, 1x Proteinase inhibitor mix and 1 mM PMSF). The samples were incubated for 4-5 h with 10 ul -Cnp1-antiserum [41] and 40 ul Protein G agarose beads (Roche). The beads were washed as described and incubated with 1 per cent SDS overnight at 65 C. The supernatant was purified using a PCR purification kit (Qiagen).
characteristics.characteristic: description
characteristics.text: complete media (YES)
characteristics.characteristic: growth condition
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4896
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: scientific_name
characteristics.text: 56_Mnase
characteristics.characteristic: title