characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-07-07T17:01:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T21:34:03Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2733672
characteristics.characteristic: External Id
characteristics.text: The Pirbright Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2015-07-07T17:01:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T21:34:03Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS534441
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2877:Delta_24h_3
characteristics.characteristic: Submitter Id
characteristics.text: exposed to deltamethrin when 3-5 days old, culled 24 h post-exposure
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: African malaria mosquito
characteristics.characteristic: common name
characteristics.text: biological replicate Protocols: Larvae were reared at low density in pans with de-ionized water and fed daily with ground Tetramin fish food. Adult mosquitoes were kept in the insectary conditions at 27 C and 12h light/12h dark cycle, with constant access to 10% sucrose solution. If not otherwise stated in the treatment section, the adults were kept at 80% relative humidity. Unmated 3-5 days old mosquitoes were exposed to deltamethrin using a WHO tube bioassay at a colony-specific LT10 (time required to kill approximately 10% of insects). The insecticide treatment was conducted in an insecticide testing room kept at 27 C and 80% RH. Following the exposure period mosquitoes were transferred to a maintenance insectary kept at 27C and 80% RH, with access to a 10% sucrose solution, until they were culled 2, 24, 48 or 72 hours post-exposure. Samples were ground in TRIzol and stored at -70 C until RNA isolation. Total RNA was isolated using PureLink RNA Mini Kit (Ambion) with an on-column DNase treatment step according to the manufacturer's protocol. The libraries were constructed with the PerkinElmer Sciclone NGS Workstation using the TruSeq RNA protocol (Illumina). The mRNA was isolated from 1 ug of total RNA by a poly-A+ pull-down using biotin beads, and fragmented. Following first- and second-strand synthesis, the cDNA fragments were blunt-ended, and then their 3 prime ends were adenylated to prevent formation of chimeric molecules during the adapter ligation. Then, adapters were ligated and the fragments were subjected to a bead-based size selection using Beckman Coulter XP beads (Beckman Coulter). Prior to hybridisation to the flow cell, the cDNA constructs were amplified by PCR to selectively enrich fragments with adapter molecules on both ends. The concentration of the libraries was determined by using a Qubit dsDNA HS Assay (Life Technologies) and qPCR.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Colonized mosquitoes derived from an eastern Ugandan population, known to have extremely high physiological resistance to pyrethroids
characteristics.characteristic: environmental history
characteristics.text: Anopheles gambiae
characteristics.characteristic: organism
characteristics.text: Anopheles gambiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7165
characteristics.characteristic: organism
characteristics.text: Anopheles gambiae
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: Delta_24h_3
characteristics.characteristic: title