characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-03-16T17:04:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:40:18Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2532528
characteristics.characteristic: External Id
characteristics.text: CR-UK Cambridge Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2015-03-16T17:04:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:40:18Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS456736
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2576:MCF-7-3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: MCF-7
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001203
characteristics.characteristic: cell line
characteristics.text: epithelial
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000066
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: MCF7 cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics. For hormone depletion, cells were grown in phenol red-free DMEM with 5% charcoal-treated FBS and 1% antibiotics for 3 days. Hormone-deprived cells were treated for 3 hours with 0.01% DMSO. Cells were fixed with 1% PFA for 10 minutes and processed as described (Schmidt et al., Methods 48:3 pp240-248 2009). For each ChIP, 10ug of antibody was used, and 100ul of Protein A magnetic beads (Invitrogen). The antibody used was raised to detect RIP140 (6D7). ChIP was performed and libaries were contructed as described (Schmidt et al., Methods 48:3 pp240-248 2009).
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: MCF-7-3
characteristics.characteristic: title
characteristics.text: RIP140
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005310
characteristics.characteristic: transcription factor