characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-11-25T17:01:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T20:14:33Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2768228
characteristics.characteristic: External Id
characteristics.text: Stowers Institute for Medical Research
characteristics.characteristic: INSDC center name
characteristics.text: 2015-11-25T17:01:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T20:14:33Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS542807
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2923:LT-HSC
characteristics.characteristic: Submitter Id
characteristics.text: 4 to 6 week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: long term hematopoietic stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002034
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: All the mice in this study were kept in C57BL/6J background. All mice used in this study were between 4-6 weeks of age and were housed in the animal facility at the Stowers Institute for Medical Research (SIMR) and handled according to SIMR and National Institute of Health (NIH) guidelines. All procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of SIMR. RNA was extracted by convention using chloroform- isopropanol trizol method. The RNA-sequencing library was prepared from approximately 200 ng of total RNA for each sample using illumina TruSeq RNA Sample Prep Kit [catalog number: FC-122-1001]). The fragment size in the generated library ranged from 220 to 500 bps with a peak at 280 bps.
characteristics.characteristic: description
characteristics.text: Lin-Sca-1+cKit+CD34-Flk2- CD49lo
characteristics.characteristic: gating
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: C57BL/6NJ
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007732
characteristics.characteristic: strain
characteristics.text: LT-HSC
characteristics.characteristic: title