characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-06-15T17:03:11Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:50:35Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2225913
characteristics.characteristic: External Id
characteristics.text: University of Manchester
characteristics.characteristic: INSDC center name
characteristics.text: 2016-06-15T17:03:11Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:50:35Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS362580
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1976:C1
characteristics.characteristic: Submitter Id
characteristics.text: 3-3.5 hour
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000032
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: The Decepentaplegic receptor Thickviens (Tkv) was over-expressed using the UASp-GAL4 system. pUASp-tkvQD was obtained from this original stock yw;pUASpTkvQD/TM6B (Casanueva and Ferguson, 2004; Development, 131(9): 1881-90). Gal4-Tub:VP16 was obtained from the original stock, Bloomington 7062. At the 3-3.5h time point after egg lay (AEL) embryos over-expressing TkvQD, derived from Gal4-Tub:VP16/+ ; P{UASp-TkvQD}/+ adults were collected. As an experimental control, 3-3.5h embryos derived from Gal4-Tub:VP16/+ ; + were also collected. RNA was extracted from 30ul of Drosophila embryos per sample using Trizol (Invitrogen) SOLiDTM Total RNA-Seq Kit (PN 4445374)
characteristics.characteristic: description
characteristics.text: larval stage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000069
characteristics.characteristic: developmental stage
characteristics.text: Gal4-Tub:VP16/+
characteristics.characteristic: genotype
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: C1
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006761
characteristics.characteristic: title