characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-05-01T17:00:44Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:08:55Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2341985
characteristics.characteristic: External Id
characteristics.text: Institute for Molecular Bioscience, University of Queensland
characteristics.characteristic: INSDC center name
characteristics.text: 2014-05-01T17:00:44Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:08:55Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS399656
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2259:liver_sample_1
characteristics.characteristic: Submitter Id
characteristics.text: 30 year
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000036
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: pre-extracted RNA was sourced commercially from BioChain Ribosomal depletion and RNA library construction was performed using Truseq Stranded Total RNA kit with Ribo-Zero Human/Mouse/Rat Set A (Illumina, Cat. #RS-122-2201).1ug of total RNA used as starting material. The library preparations were performed according to the manufacturer's protocol (Truseq Stranded Total RNA Sample Prep Guide Pages 58-87) with minor modifications to the fragmentation and the number of PCR cycles (from the recommended 15 down to 8 in order to increase library fragment complexity). After the addition of elute, fragment and high mix to the ribosomal RNA-depleted RNA, samples were incubated on ice and followed immediately by first strand synthesis. The DNA fragments were amplified and the cleaned libraries quantified using high sensitivity Bioanalyzer.
characteristics.characteristic: description
characteristics.text: NB0030
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: liver_sample_1
characteristics.characteristic: title