characteristics.text: 2011-11-26T17:00:49Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:33:30Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1094613
characteristics.characteristic: External Id
characteristics.text: GATC Biotech AG
characteristics.characteristic: INSDC center name
characteristics.text: 2011-11-26T17:00:49Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:33:30Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS075001
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-861:Central starchy endosperm 5
characteristics.characteristic: Submitter Id
characteristics.text: 28 d
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: bread wheat
characteristics.characteristic: common name
characteristics.text: Protocols: Heads were tagged at anthesis and pure starchy endosperm dissected at 10, 14, 17, 21, and 28dpa for transcript analysis. The outside two florets on the central four spikelets on each side of the ear, in total 16 caryopses, were dissected to yield pure central starchy endosperm of the developing grain. Each head was considered a biological replicate for the qRT analysis, and up to 10 replicates were pooled for the RNA sequencing and biochemical analysis. Dissected samples were immediately frozen in liquid nitrogen, lyophilised, and homogenised in a TissueLizer (Qiagen). Bread wheat (Triticum aestivum) cv Cadenza plants were grown in controlled environment as described in Sparks and Jones, 2009, (In HD Jones, PR Shewry, eds, Transgenic Wheat, Barley and Oats. Humana Press, pp 71-92).
characteristics.characteristic: description
characteristics.text: anthesis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007016
characteristics.characteristic: initial time point
characteristics.text: Triticum aestivum
characteristics.characteristic: organism
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: starchy endosperm
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0006220
characteristics.characteristic: organism part
characteristics.text: Triticum aestivum
characteristics.characteristic: scientific_name
characteristics.text: Central starchy endosperm 5
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0006220
characteristics.characteristic: title