characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-10-25T17:01:04Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T21:10:40Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2765968
characteristics.characteristic: External Id
characteristics.text: DKFZ/ZMBH Alliance, University of Heidelberg, Heidelberg, Germany
characteristics.characteristic: INSDC center name
characteristics.text: 2014-10-25T17:01:04Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T21:10:40Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS542745
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2915:A3 UAS-esg no3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: MyoIAts > UASt-esg Protocols: MyoIAts was used to drive transgene expression for 24 hours at 29 degrees C. 25 adult female intestines per genotype were dissected and midguts were homogenized in Trizol (Life Technologies). RNA was isolated by Phenol/Chloroform extraction, followed by purification of the RNA containing fractions using RNAeasy columns supplied with the RNAeasy Mini Kit (Qiagen). RNA was Poly-A-selected. RNA integrity was determined with an Agilent 2100 Bioanalyzer before library generation. cDNA libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB), and NEBNext Multiplex Oligos for Illumina (NEB) were used for multiplexing.
characteristics.characteristic: description
characteristics.text: UAS-esg overexpression
characteristics.characteristic: genotype
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: A3 UAS-esg no3
characteristics.characteristic: title