characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-08-31T17:01:42Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T19:58:17Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2649654
characteristics.characteristic: External Id
characteristics.text: EBI
characteristics.characteristic: INSDC center name
characteristics.text: 2014-08-31T17:01:42Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T19:58:17Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS506504
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2754:H3.3-3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: DT40
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006274
characteristics.characteristic: cell line
characteristics.text: lymphocyte of B lineage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000945
characteristics.characteristic: cell type
characteristics.text: chicken
characteristics.characteristic: common name
characteristics.text: Protocols: Chicken DT40 cells were grown in RPMI-1640 with 7% FBS, 3% chicken serum and supplemented with pen/strep at 37C with 10% CO2. The source (starting sample for the experiment) were wild type and H3.3 knockout DT40 cells. RNA was extracted using an RNeasy Mini Kit (Qiagen). The RNA quality and quantity was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies). Libraries were constructed by BGI (Beijing Genomics Institute)
characteristics.characteristic: description
characteristics.text: h3.3a-/-, h3.3b-/-
characteristics.characteristic: genotype
characteristics.text: Gallus gallus
characteristics.characteristic: organism
characteristics.text: Gallus gallus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9031
characteristics.characteristic: organism
characteristics.text: Gallus gallus
characteristics.characteristic: scientific_name
characteristics.text: H3.3-3
characteristics.characteristic: title