characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-01-19T17:03:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T20:52:53Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2757651
characteristics.characteristic: External Id
characteristics.text: The Roslin Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2015-01-19T17:03:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T20:52:53Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS541536
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2910:Smith_Roslin_IBDV_con_rep3.fq.gz
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: chicken
characteristics.characteristic: common name
characteristics.text: Protocols: Birds were separated into two experimental rooms, with ad libitum access to food and water. In one room, each bird was mock-infected with 100 ul PBS intra-nasally. In the other room, each bird was infected intra-nasally with 101.3 embryonic infectious dose 50 (EID50) (in 100 µl PBS) of the virulent IBDV strain F52/70. Birds were monitored for clinical signs for 4 dpi and killed at 2, 3 and 4 dpi. Spleen and bursa were collected from all birds. Tissue samples were stabilized in RNAlater (Qiagen, Crawley, UK) and disrupted using a bead mill (Retsch MM 300, Retsch, Haan, Germany) at 20 Hz for 4 min. Total RNA was prepared using a Trizol (Invitrogen, Paisley, UK) extraction method as per the manufacturer's protocol. Samples were resuspended in a final volume of 100 ul of RNAse-free water. Samples were then passed through RNeasy columns (Qiagen) to clean up the RNA further. Concentrations of the samples were calculated by measuring OD260 and OD280 on a spectrophotometer. Quality of the RNA was checked on a bioanalyser (Agilent Technologies, South Queensferry, UK). Samples were prepared for mRNA sequencing using 5ug of total RNA starting material following the Illumina mRNA sequencing 8 sample preparation kit protocol.
characteristics.characteristic: description
characteristics.text: Gallus gallus
characteristics.characteristic: organism
characteristics.text: Gallus gallus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9031
characteristics.characteristic: organism
characteristics.text: spleen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0001281
characteristics.characteristic: organism part
characteristics.text: Gallus gallus
characteristics.characteristic: scientific_name
characteristics.text: 4 day
characteristics.characteristic: time
characteristics.text: Smith_Roslin_IBDV_con_rep3.fq.gz
characteristics.characteristic: title