characteristics.text: 2013-08-13T17:00:27Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:29:46Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2078263
characteristics.characteristic: External Id
characteristics.text: Stowers Institute for Medical Research
characteristics.characteristic: INSDC center name
characteristics.text: 2013-08-13T17:00:27Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:29:46Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS252142
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1628:MPP dH19
characteristics.characteristic: Submitter Id
characteristics.text: 6 week
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000034
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: MPP (Lin- Sca1+ cKit+ CD34+ Flk2+)
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: All mice used in this study were housed in the animal facility at the Stowers Institute for Medical Research (SIMR) and handled according to SIMR and National Institutes of Health (NIH) guidelines. All procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of SIMR. H19-DMR flDMR/flDMR mice on B6 background were kindly provided by Dr. Marisa S. Bartolomei (University of Pennsylvania Perelman School of Medicine). Conditional mutant Igf1rfl/+ was kindly provided by Thomas L Clemens (Center for Musculoskeletal Research, Johns Hopkins Medicine, 601 North Caroline Street, Baltimore, MD 21287). Interferon inducible Mx1-Cre or tamoxifen inducible Scl-Cre mouse strains were used to delete the floxed H19-DMR and Igf1r. For Mx1-Cre activation, 250ug of pIpC was injected intraperitoneal every other day for 14 days at 5 weeks of age. For Scl-CreER activation, 2mg tamoxifen dissolved in 0.1 ml of corn oil was injected intraperitoneal every day for 5 days. Illumina TruSeq RNA Sample Prep Kit (Catalog #: FC-122-1001) The RNA-sequencing library was prepared from approximately 200 ng of total RNA (mH19dDMR/+ Igf1r-/- , mH19dDMR/+Igf1rf-/- and mH19flDMR/+) for each sample. The fragment size in the generated library ranged from 220 to 500 bps with a peak at 280 bps.
characteristics.characteristic: description
characteristics.text: mH19 flDMR/+
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: FACS-sorted bone marrow
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: C57BL6
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004472
characteristics.characteristic: strain
characteristics.text: MPP dH19
characteristics.characteristic: title