characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-11-25T17:01:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T22:25:36Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3134078
characteristics.characteristic: External Id
characteristics.text: Stowers Institute for Medical Research
characteristics.characteristic: INSDC center name
characteristics.text: 2015-11-25T17:01:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T22:25:36Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS606051
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3079:T Cell
characteristics.characteristic: Submitter Id
characteristics.text: 4 to 6 week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: T cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000084
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: All the mice in this study were kept in C57BL/6J background. All mice used in this study were between 4-6 weeks of age and were housed in the animal facility at the Stowers Institute for Medical Research (SIMR) and handled according to SIMR and National Institute of Health (NIH) guidelines. All procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of SIMR. RNA was extracted by convention using chloroform-isopropanol trizol method. The RNA-sequencing library was prepared from approximately 20 ng of total RNA for each sample using illumina TruSeq Small RNA Sample Preparation Kit [catalog number: RS-200-0012]). The fragment size in the generated library ranged from 140 to 300 bps.
characteristics.characteristic: description
characteristics.text: CD3+, CD4+, CD8+, B220-, IgM-, Ter119-, Gr1-, Mac1-
characteristics.characteristic: gating
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: T Cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000084
characteristics.characteristic: title