characteristics.text: 2013-03-29T17:01:40Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:20:54Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1714533
characteristics.characteristic: External Id
characteristics.text: Institute for Systems Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2013-03-29T17:01:40Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:20:54Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS226508
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1571:Sample14
characteristics.characteristic: Submitter Id
characteristics.text: 8 week
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000034
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mice were housed in laminar flow cages and permitted ad lib access to sterile food and water. Mice were exposed to aerosolized bacteria in a nose-only inhalation chamber (In-Tox products, Moriarty, NM) using a computer interface to control pressures and flows (Biaera Technologies, Frederick MD). To characterize the early course of each infection, 3-4 mice were euthanized after 4, 24 and 48hrs with intraperitoneal pentobarbital and exsanguinated by external cardiac puncture. Ten micrograms of total RNA isolated from lung tissue (equal amounts pooled from 3 individual animals) was sequentially treated with MICROBEnrich and MICROBExpress kits (Ambion) to enrich for bacterial mRNA through removal of mammalian mRNA/rRNA and bacterial rRNA, respectively. The resultant bacterial mRNA was fragmented using a RNA fragmentation kit (Ambion), which yielded fragments of 60-200 nucleotides in length. Libraries for Illumina sequencing were prepared as recommended by Illumina.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: lung
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002048
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: Balb/c
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000602
characteristics.characteristic: strain
characteristics.text: 4 hour
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000032
characteristics.characteristic: time post F. tularensis SchuS4 infection
characteristics.text: Sample14
characteristics.characteristic: title