characteristics.text: 2012-09-04T09:38:46Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:40:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SRA-Webin
characteristics.characteristic: ENA-SUBMISSION-TOOL
characteristics.text: SAMEA1500965
characteristics.characteristic: External Id
characteristics.text: IPK-Gatersleben
characteristics.characteristic: INSDC center name
characteristics.text: 2012-09-04T09:38:46Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:40:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS104124
characteristics.characteristic: SRA accession
characteristics.text: glumes
characteristics.characteristic: Submitter Id
characteristics.text: domesticated barley
characteristics.characteristic: common name
characteristics.text: Barley (Hordeum vulgare L.) plants of cv. Barke were grown under standard greenhouse conditions at 18 degree Celsius with 16 h of light. Developmental stages for barley grains were determined after Weschke et al. (2000). Flag leaves, glumes and grain tissues were collected based on grain developmental stages. For flag leaves and glumes, samples were collected in two day interval starting from 4 days before anthesis until 24 days after flowering (DAF). Grains from 0, 4, 8, 10, 12, 14 DAF were manually dissected into the maternal and the filial parts and whole caryopses were sampled at 16, 20 and 24 DAF. Total RNA was isolated separately from each tissue and stage using Purescript RNA isolation kit (Biozym, Hamburg, Germany). To prepare RNA samples for transcriptome sequencing of flag leaves, glumes and grains, equal amounts of RNA from all stages were united for each tissue to achieve 27 microgramme of RNA for each organ. Pyrosequencing of the three libraries was done by GATC Biotech (www.gatc-biotech.com).
characteristics.characteristic: description
characteristics.text: Hordeum vulgare subsp. vulgare
characteristics.characteristic: organism
characteristics.text: Hordeum vulgare subsp. vulgare
characteristics.characteristic: scientific_name
characteristics.text: Membranous bracts at the base of a cereal seed (PO:0006367).
characteristics.characteristic: title