characteristics.text: Pilot1
characteristics.characteristic: 1000 Genomes dataset
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2013-10-17T17:02:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:47:21Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2223477
characteristics.characteristic: External Id
characteristics.text: University of Geneva Medical School, Department of Genetic Medicine and Development
characteristics.characteristic: INSDC center name
characteristics.text: 2013-10-17T17:02:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:47:21Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS359938
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1883:NA11830
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: NA11830
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000895
characteristics.characteristic: cell line
characteristics.text: lymphoblastoid
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000572
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Lymphoblastoid cell lines were obtained from Coriell (http://ccr.coriell.org) and cultured in RPMI-1640 medium (Lonza) with 10% fetal calf serun (FCS). Frozen cells were thawed and transferred to T25 flasks containing 15 ml of medium. At a density of 0.3 x 10^6 cells/m cells were transferred to TubeSpin Bioreactor 50 tubes (TPP) in 5 ml of same medium containing 10% FCS and 0.1% Pluronic F-68 (Sigma-Aldrich). The cultures were agitated at 180 rpm with 5% CO2 and 85% humidity. When the cell density reached 2-3 x 10^6 cells/ml, the culture was diluted to 0.3 x 10^6 cells/ml and transferred to a 250-ml glass bottle. The culture was agitated at 110 rpm with 5% CO2 but no humidity. Eventually, the cells were scaled-up serially in 500-ml, 1-L, and 5-L glass bottles filled to a maximum of 40% of the nominal volume. Total RNA was extracted from flash frozen cell pellets using the TRIzol Reagent (Invitrogen) according to manufacturer's instructions. No DNAse treatment of the RNA samples was performed. Total RNA quality was assessed with Bioanalyzer RNA 6000 Nano Kit (Agilent) and RNA quantity was measured with Qubit 2.0 (Invitrogen) RNA Broad range kit according to manufacturer's instructions. Sequencing libraries were prepared with TruSeq RNA Sample Prep Kit v2 high-throughput protocol (Illumina) according to manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: NA11830
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000895
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: HapMap CEU
characteristics.characteristic: population
characteristics.text: CEPH
characteristics.characteristic: population name
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: NA11830
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000895
characteristics.characteristic: title