characteristics.text: 2012-12-31T17:01:41Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:47:02Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SRA-Webin
characteristics.characteristic: ENA-SUBMISSION-TOOL
characteristics.text: SAMEA2008521
characteristics.characteristic: External Id
characteristics.text: BCCS
characteristics.characteristic: INSDC center name
characteristics.text: 2012-12-31T17:01:41Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:47:02Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS148507
characteristics.characteristic: SRA accession
characteristics.text: Nipbl
characteristics.characteristic: Submitter Id
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: characteristics = 1-7HB2 cells(Bartek et al. 1991) characteristics = Chromatin immunoprecipitation for NIPBL growth_protocol = HB2 cells (1-7HB2, a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7, (Bartek et al. 1991)) were cultured in DMEM supplemented with 0.2 mM L-glutamine, 100 units/ml penicillin, 100 mg/ml streptomycin, 10% FCS, 5 �g/ml hydroxycortisone and 10 �g/ml human insulin. For the ChIP cells were enriched in G1 phase by a double thymidine block-release protocol. NIPBL_description = Polyclonal rabbit anti-NIPBL antibody raised against residues 2598-2825 of the X. laevis Scc2-1B and purified using the epitope used for immunization (133M, gift from Koichi Tanaka and Kim Nasmyth) was used for ChIP as described in (Wendt, K.S., Yoshida, K., Itoh, T., Bando, M., Koch, B., Schirghuber, E., Tsutsumi, S., Nagae, G., Ishihara, K., Mishiro, T., Yahata, K., Imamoto, F., Aburatani, H., Nakao, M., Imamoto, N., Maeshima, K., Shirahige, K., and Peters, J.M. 2008. Cohesin mediates transcriptional insulation by CCCTC-binding factor. Nature 451(7180): 796-801.).
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Nipbl Chip Seq sample
characteristics.characteristic: title