characteristics.text: GSC:MIMARKS:2.1
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2012-03-22T13:21:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: GSC, MIxS, MIMARKS
characteristics.characteristic: ENA-KEYWORD
characteristics.text: 2018-03-09T09:52:42Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: GSC:MIENS_S:2.1:compliant
characteristics.characteristic: ENA-STANDARD
characteristics.text: SAMEA1021303
characteristics.characteristic: External Id
characteristics.text: UBP-CNRS
characteristics.characteristic: INSDC center name
characteristics.text: 2012-03-22T13:21:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-09T09:52:42Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: water
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_15377
characteristics.characteristic: MIGS env_package
characteristics.text: ERS015567
characteristics.characteristic: SRA accession
characteristics.text: MET6
characteristics.characteristic: Submitter Id
characteristics.text: 20
characteristics.unit: liter
characteristics.characteristic: amount or size of sample collected
characteristics.text: 2008-06-26
characteristics.characteristic: collection date
characteristics.text: 20 litres were collected from Lake Pavin at the 5m-depth. Sample was successively filtrated (25 μm, 1,2 μm, 0,2 μm) and concentrated by tangential ultra-filtration. Capsid particles were then concentrated by pegylation, and treated by DNAse. Viral DNA was freed via a thermal chock, purified (kit Quiamp DNA, Quiagen), and amplified (kit GE Healthcare, Genomiphi).
characteristics.characteristic: description
characteristics.text: 12.12
characteristics.unit: milligrams per litre
characteristics.characteristic: dissolved oxygen
characteristics.text: small lake biome;lake;pond
characteristics.characteristic: environment
characteristics.text: 1197
characteristics.unit: meter
characteristics.characteristic: geographic location (altitude/elevation)
characteristics.text: France:Auvergne:Lac Pavin
characteristics.characteristic: geographic location (country)
characteristics.text: 5
characteristics.unit: meter
characteristics.characteristic: geographic location (depth)
characteristics.text: 45.495833, 2.888333
characteristics.characteristic: geographic location (latitudeandlongitude)
characteristics.text: 684,224
characteristics.characteristic: library reads sequenced
characteristics.text: Genomiphi GE Healthcare
characteristics.characteristic: nucleic acid amplification
characteristics.text: Quiagen Quiamp DNA mini kit
characteristics.characteristic: nucleic acid extraction
characteristics.text: freshwater metagenome
characteristics.characteristic: organism
characteristics.text: 7.98
characteristics.characteristic: ph
characteristics.text: bottle
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0010132
characteristics.characteristic: sample collection device or method
characteristics.text: filtering, pegylation
characteristics.characteristic: sample material processing
characteristics.text: freshwater metagenome
characteristics.characteristic: scientific_name
characteristics.text: pyrosequencing
characteristics.characteristic: sequencing method
characteristics.text: 13.59
characteristics.unit: Celsius
characteristics.characteristic: temperature
characteristics.text: Viral metagenome from Lake Pavin (MET6).
characteristics.characteristic: title