characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-04-06T17:00:42Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:12:34Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2382527
characteristics.characteristic: External Id
characteristics.text: University of Edinburgh
characteristics.characteristic: INSDC center name
characteristics.text: 2014-04-06T17:00:42Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:12:34Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS408852
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2330:PSLN_K277
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: sheep
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/LBO_0001041
characteristics.characteristic: common name
characteristics.text: Protocols: New Zealand Cheviot sheep of PRNP genotypes VRQ/VRQ, from the DEFRA breeding flock were inoculated subcutaneously with 2 ml of 10% w/v brain homogenate into sites on either side of the neck roughly 10cm anterior to the prescapular lymph node (PSLN). In all experiments frozen lymph node was converted to a frozen tissue powder. Briefly a 100 mg of tissue per sample was placed into a Teflon flask along with steel ball and placed in liquid Nitrogen for 1 min. Homogenised for 60 seconds at 2000 rpm in a Mikro-Dismembrator U (B.Braun Biotech International GmbH). Total RNA was extracted from the frozen tissue powder using the RiboPure Kit (Ambion) including a DNase I digestion. To the frozen tissue powder 1 ml of Tri Reagent was added and then homogenise for a further 15 seconds at 1500rpm. A 200 ul aliquot of chloroform was added to lysate and mixed vigorously by vortexing. The protocol was then followed as per the manufactures instructions. The RNA was eluted in a final volume of 50 ul. Each RNA sample was DNase I (Ambion) treated as per the manufacturers instructions. The quality and quantity of the isolated total RNA was assessed using a RNA LabChip on the Agilent 2100 bioanalyser and a NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies Inc.). (
characteristics.characteristic: description
characteristics.text: lamb
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000112
characteristics.characteristic: developmental stage
characteristics.text: SSBP/1 infected
characteristics.characteristic: disease
characteristics.text: Ovis aries
characteristics.characteristic: organism
characteristics.text: Ovis aries
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9940
characteristics.characteristic: organism
characteristics.text: prescapular lymph node
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0016382
characteristics.characteristic: organism part
characteristics.text: Ovis aries
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: PSLN_K277
characteristics.characteristic: title