characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-08-07T17:02:02Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T22:21:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3120839
characteristics.characteristic: External Id
characteristics.text: University of Manchester
characteristics.characteristic: INSDC center name
characteristics.text: 2015-08-07T17:02:02Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T22:21:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS604985
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3084:Input DNA
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: U2OS
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002869
characteristics.characteristic: cell line
characteristics.text: osteosarcoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000637
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: The U2OS cells were grown in DMEM supplement with 10% foetal bovine serum. Cells were cross-linked with Formaldehyde for 10 minutes. After reverse cross-link and protein digestion with Proteinase K (Roche), DNA was purified using Qiaquick, PCR purification kit (Qiagen) following the manufacturer’s instructions. ChIP-seq was performed as described in Schmidt et al., 2009, using 3 x 10^6 U2OS. Immuno-precipitation was performed overnight at 4° C by incubating the shared DNA-protein complex with3 micro-gram of anti-SUMO2 (Life technologies) antibody previously coupled to Dynabeads protein A (Invitrogen). Schmidt D, Wilson MD, Spyrou C, Brown GD, Hadfield J, Odom DT. (2009) ChIP-seq: using high-throughput sequencing to discover protein-DNA interactions. Methods 48: 240-248. Libraries were generated following manufacturer's protocol.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Input DNA
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005031
characteristics.characteristic: title