characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2021-02-19T04:08:52Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T19:16:29Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2700109
characteristics.characteristic: External Id
characteristics.text: Vanderbilt University
characteristics.characteristic: INSDC center name
characteristics.text: 2021-02-19T04:08:52Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T19:16:29Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS527843
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2816:P60 MIP-GFP mice 2
characteristics.characteristic: Submitter Id
characteristics.text: postnatal day 60
characteristics.characteristic: age measurement datum
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: 2 adult P60 MIP-GFP mice Protocols: Embryos were isolated from mice; the presence of vaginal plug at noon was considered as E0.5. Embryos obtained from heterozygous interbreeding were genotyped by fluorescence microscopy or were genotyped using PCR. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating.
characteristics.characteristic: description
characteristics.text: post-juvenile adult stage
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: life cycle stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: STOCK Tg(Ins1-EGFP/GH1)14Hara/Mmmh mouse strain
characteristics.characteristic: strain
characteristics.text: P60 MIP-GFP mice 2
characteristics.characteristic: title