characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-01-21T17:01:32Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:18:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2421744
characteristics.characteristic: External Id
characteristics.text: School of Agriculture, Policy and Development, The University of Reading, UK
characteristics.characteristic: INSDC center name
characteristics.text: 2016-01-21T17:01:32Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:18:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS426828
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2403:SE_10D
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Immature zygotic embryos collected after 10 d of inoculation on B5 medium supplemented with 5 uM 2, 4-dichlorophenoxyacetic acid Protocols: Somatic embryos were induced on B5 medium supplemented with 5 uM 2, 4-dichlorophenoxyacetic acid (Gaj, 2001). In vitro cultures were maintained at 24C under a 16 h/ 8 h (light/dark) cycle in a culture room. Samples were collected after 10 d of in vitro culture. Approximately 700 embryogenic cultures induced at each time-point were pooled together to extract total RNA. Total RNA was extracted using the RNeasy® Plant Mini Kit (Qiagen, UK) according to the manufacturers protocol. The cDNA libraries were constructed in accordance with the Illumina TruSeq RNA sample preparation guide v2 for Illumina paired-end multiplexed sequencing.
characteristics.characteristic: description
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: somatic embryo
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: SE_10D
characteristics.characteristic: title