characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-03-03T16:32:10Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:59:02Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2267171
characteristics.characteristic: External Id
characteristics.text: Sapienza Universita di Roma
characteristics.characteristic: INSDC center name
characteristics.text: 2014-03-03T16:32:10Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:59:02Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS374638
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2128:HeLaS3_rep2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HeLa-S3
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002791
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: HeLaS3 cells were grown in DMEM supplemented with 10%FBS, pen/strep and L-glutamine. HeLaS3 cells were transfected with 10nMol/l siRNA using Interferin reagent (Polyplus) according to manufacturer instructions. Total RNA was extracted using Tri-reagent according to manufacturers protocol (Sigma). mRNA libraries were constructed using the TruSeq RNA Sample Preparation Kits v2 (Illumina) starting from total RNA
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: HeLaS3_rep2
characteristics.characteristic: title