characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-01-07T17:01:29Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T21:45:03Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3143111
characteristics.characteristic: External Id
characteristics.text: CeMM - Center for Molecular Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2015-01-07T17:01:29Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T21:45:03Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS614153
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3102:K562_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: K562
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002067
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: HEK293 and K562 cells were grown in DMEM and IMDM, respectively and supplemented with 10% fetal bovine serum. RNA was extracted using TRI reagent (SIGMA), then it was DNAse I treated (DNA free kit from Ambion), then it was treated with RiboZero kit (Epicentre) to remove ribosomal RNA. The library was prepared using ScriptSeq kit version 1 (Epicentre).
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: K562_2
characteristics.characteristic: title