characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-05-20T17:01:22Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T18:57:47Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2633611
characteristics.characteristic: External Id
characteristics.text: EBI
characteristics.characteristic: INSDC center name
characteristics.text: 2016-05-20T17:01:22Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T18:57:47Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS500843
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2590:wee1-1/rnh2a - 2mM HU - 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: The WEE1 knockout (wee1-1) and the RNH2A/WEE1 double knockout (rnh2a/wee1-1) seeds were germinated on control medium on a nylon mesh. Transferred 5 d after germination to control medium or medium supplemented with 2 mM HU 24h after the transfer 200 root tips (<2 to 3 mm) were collected and frozen in liquid nitrogen. RNA was extracted from root tissue with the RNeasy plant kit (Qiagen). Illumina True-Seq libraries were generated from cDNA according to the manufacture’s protocol and sequenced on a HiSeq2000.
characteristics.characteristic: description
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: root tip
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0000025
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: wee1-1/rnh2a
characteristics.characteristic: strain
characteristics.text: wee1-1/rnh2a - 2mM HU - 2
characteristics.characteristic: title