characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-04-22T17:00:44Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:12:09Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2358425
characteristics.characteristic: External Id
characteristics.text: University of Algarve
characteristics.characteristic: INSDC center name
characteristics.text: 2014-04-22T17:00:44Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:12:09Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS403451
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2321:wildtype_rep1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: Flies were raised using standard techniques. The fandango alleles were isolated from a previously reported maternal screen (Pimenta-Marques et al., 2008). Flies were raised using standard techniques. Total RNA was isolated from 0-3Hs collections of fandango maternal mutant and control (FRT42B) embryos using TRIzol Reagent (Invitrogen) following standard protocol. To the RNA samples was performed a DNAse I (Promega) treatment during 30 min at 37C. After, the DNAse was extracted by Phenol-Chloroform extraction, the RNA was pellet by ethanol precipitation and dissolved it in 25 ul of DEPC water. We performed a Bioanalyzer test to analyze the quality and concentration of the samples and finally we brought the volume to 100 ul with water, added 50 ul of 7.5 M NH4OAc, 0.5 ul of glycogen and 250 ul of absolute ethanol. cDNA library was generated using the standard Illumina protocol for RNA-seq
characteristics.characteristic: description
characteristics.text: embryo stage
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: FRT42B
characteristics.characteristic: strain
characteristics.text: wildtype_rep1
characteristics.characteristic: title