characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-12-11T17:01:51Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T21:23:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2747167
characteristics.characteristic: External Id
characteristics.text: Iowa State University
characteristics.characteristic: INSDC center name
characteristics.text: 2014-12-11T17:01:51Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T21:23:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS539975
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2886:MM02 PT
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: zebrafish
characteristics.characteristic: common name
characteristics.text: Protocols: isolation and care of zebrafish transgenic line Tg(flk1:RFP)is18 Tissues for isolation of total RNA for RNA-Seq libraries were dissected from three age-matched genotypes. Total RNA was isolated using Qiagen RNeasy RNA Isolation Kit (Qiagen). The first sample, “Wild Type”, contained three pooled retinas from 6-month-old wild type adults. The second sample, “Pretumor”, consisted of three pooled retinas from age-matched heterozygous Tg(flk1:RFP)is18/+ adults that did not show obvious gross ocular tumors. The third sample, “Tumor”, consisted of tumor tissue dissected from the eyes of two age-matched heterozygous Tg(flk1:RFP)is18/+ adults that had advanced, large tumors that filled the vitreous and distorted the ocular cavity. RNA-Seq libraries were prepared from each sample and 100 bp paired-end sequenced in individual lanes on an Illumina HiSeq 2000 instrument at the Genome Sequencing and Analysis Core Resource, Duke Institute for Genome Sciences and Policy, Duke University.
characteristics.characteristic: description
characteristics.text: Tg(flk1:RFP)is18/+
characteristics.characteristic: genotype
characteristics.text: Danio rerio
characteristics.characteristic: organism
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: retina
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000966
characteristics.characteristic: organism part
characteristics.text: Danio rerio
characteristics.characteristic: scientific_name
characteristics.text: MM02 PT
characteristics.characteristic: title