characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-03-01T17:02:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T20:21:13Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2771241
characteristics.characteristic: External Id
characteristics.text: Department of Molecular Neuroscience, UCL Institute of Neurology
characteristics.characteristic: INSDC center name
characteristics.text: 2015-03-01T17:02:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T20:21:13Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS543989
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2937:hiCLIP_STAU1_LUs27
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HEK293
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001182
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: A FlpIn293 cell line expressing 3xFLAG-STAU1 was subjected to UV-C irradiation before collection. hiCLIP or iCLIP protocol was followed to isolate RNA bound to 3XFLAG-STAU1 protein and amplify cDNA library as described in the manuscript. Specific barcodes were used for each RT reaction, and are specified in the manuscript. Random barcodes were also incorporated into cDNAs to distinguish between PCR duplication of cDNA products. The final products were obtained by PCR with Illumina paired-end sequencing primers: 5-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3; 5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: hiCLIP_STAU1_LUs27
characteristics.characteristic: title