characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-07-18T14:41:58Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:16:32Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2417164
characteristics.characteristic: External Id
characteristics.text: MRC-NIMR
characteristics.characteristic: INSDC center name
characteristics.text: 2014-07-18T14:41:58Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:16:32Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS423625
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2268:W4_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: mouse embryonic stem cell
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004038
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mouse ES cells were grown as monolayer in N2B27 medium supplemented with 10ng/ml bFGF (R&D) for 3 days (D1-D3) and then were transferred into serum free media without bFGF (D3-D5).In these conditions cells acquired and anterior neural identity (NA). To induce ventral hindbrain identity NPCs (NH), 100nM RA (Sigma) and 500nM SAG (Calbiochem) was added from D3-D5. Spinal cord identity (NP) was induced by the addition of 5nM CHIR 99021 (Axon) from D2 to D3 followed by 100nM RA, 500nM SAG from D3-D5. To induce mesodermal differentiation the cells were treated with 5uM CHIR99021 from D2-D5 (Meso). RNA samples were collected each day of differentiation from D1 to D5 for the different conditions (NA,NH,NP and Meso). Total RNA was processed according to the TruSeq protocol (Illumina). Three separate read RNA libraries (biological replicates) were barcoded and prepared for each time point. Library size, purity and concentration were determined using Agilent Technologies 2100 Bioanalyzer.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: W4_2
characteristics.characteristic: title