characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-07-18T15:34:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:02:21Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2292774
characteristics.characteristic: External Id
characteristics.text: Department of Plant and Environmental Sciences/CIGENE, Norwegian University of Life Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2014-07-18T15:34:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:02:21Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS386438
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2137:Wheat_Room2_SE_30DPA_Rep
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: bread wheat
characteristics.characteristic: common name
characteristics.text: Chinese Spring
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007588
characteristics.characteristic: cultivar
characteristics.text: Protocols: Bread wheat cv Chinese Spring plants were grown in phytotrone chambers with cycles of 16 h of light at 20C and darkness of 8h at 15C. Each room held 75 pots with three plants each. Plants were tagged at anthesis and the middle part of ears were harvested at 10, 20 and 30 DPA and stored at -80C. Seeds were dissected using dry ice and covered by RNAlater-ICE (Life Technologies, NY, USA) under the dissecting microscope. Embryos were removed and grains cut in slices for isolation of the aleuron layer, transfer cells and starchy endosperm. Dissected tissues were frozen in liquid nitrogen and stored at -80C for subsequent RNA isolation. Tissues from 15 pots per room were pooled before RNA isolation. Two replicate samples were made per room, giving a total of four replicates per tissue and time points. Total RNA was extracted from frozen plant material using the RNeasy Lipid Tissue Mini kit (QIAGEN, Hilden, Germany; http://www.qiagen.com/applications/plant). Illumina TruSeq RNA Sample Preparation Kits v2
characteristics.characteristic: description
characteristics.text: 30 days post anthesis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007016
characteristics.characteristic: developmental stage
characteristics.text: Triticum aestivum
characteristics.characteristic: organism
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: starchy endosperm
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0006220
characteristics.characteristic: organism part
characteristics.text: Triticum aestivum
characteristics.characteristic: scientific_name
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: Wheat_Room2_SE_30DPA_Rep
characteristics.characteristic: title