characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-04-07T17:00:43Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:10:12Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2341695
characteristics.characteristic: External Id
characteristics.text: Universidade Federal do Para
characteristics.characteristic: INSDC center name
characteristics.text: 2014-04-07T17:00:43Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:10:12Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS399366
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2273:Gastric_Antrum_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Fresh tissue sample for ultra deep sequencing was obtained from a gastroscopic biopsy (0.4 mm3). The patient was 33 years old, with no evidence of cancer and a normal gastroesophageal junction. Histological examination confirmed normal and healthy conditions. The total small RNA was collected from the tissue samples using the mirVana Isolation Kit (Ambion Inc., US). The concentration and quality were determined using a Nanodrop 1000 spectrophotometer (ND-1000; Nanodrop Technologies, Wilmington, DE), and purification and size selection were performed using 6% polyacrylamide gel electrophoresis Using the SOLiD Small RNA Expression Kit (Ambion Inc., US), 200 ng of small RNA (150-200 bp) was used as a template for the miRNA library. The miRNAs in the library were tagged with unique and specific amplification primers (The barcode system Life Technologies, CA, US). Next, 50 pg of the library was pooled with seven additional miRNA libraries at the same concentration. A fraction of the library pool (0.1 pg) was amplified and fixed on magnetic beads using emulsion PCR. The ePCR product was deposited onto a single slide, and multiplex SOLiD sequencing was used.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: pyloric antrum
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Gastric_Antrum_1
characteristics.characteristic: title