characteristics.text: 2014-02-14T17:02:00Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:10:48Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2042414
characteristics.characteristic: External Id
characteristics.text: University of Marburg
characteristics.characteristic: INSDC center name
characteristics.text: 2014-02-14T17:02:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:10:48Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS197250
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1394:SAOS+p53RR
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: SAOS
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: SAOS cells were cultured in Dulbecco's modified Eagle's medium (Life Technologies) supplemented with 10 % fetal bovine serum (PAA) and penicillin/streptomycin (Life Technologies) using standard conditions and procedures. Cells were transduced with recombinant adenovirus containing p53 wildtype, p53 R181E mutant, p53 E180R mutant, p53 R181E_E180R double mutant or GFP as previously described (Cam et al., 2006). ChIP was perfomed with a p53-specific antibody. The DNA was purified with the Qiaquick PCR purification Kit (Qiagen).
characteristics.characteristic: description
characteristics.text: Anti-p53, sc-126, Santa-cruz
characteristics.characteristic: immunoprecipitate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: SAOS+p53RR
characteristics.characteristic: title
characteristics.text: RR
characteristics.characteristic: transfection