characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-05-15T17:02:21Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:22:00Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2464865
characteristics.characteristic: External Id
characteristics.text: University of Toronto
characteristics.characteristic: INSDC center name
characteristics.text: 2014-05-15T17:02:21Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:22:00Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS434671
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2498:cell_culture_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HEK293
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001182
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: 6.3x10e6 HEK293_M2 cells harboring the virally integrated human ORFeome version 3.1 collection were thawed and cultured for 1 day to leave the cells time to recover. Genomic DNA was recovered from 16x10e6 cells and 6 micrograms of gDNA were used to amplify the collection (3 PCR reactions with 2 micrograms of gDNA). Cells were lysed using SNET buffer (10mM Tris pH 8.0, 0.1M EDTA, 0.5% SDS, 0.1mg/ml Proteinase K, 25microgram/ml RNAse A). Genomic DNA was isolated using 1 volume of Phenol/Chloroform/Isoamyl Alcohol (25:24:1) pH 8.0, precipitated by adding 2 volumes of 95% Ethanol (-20C), washed with ice cold 70% Ethanol and resuspended in Tris-HCl pH 8.0. Following PCR, the amplified ORFs were used directly to prepare a Nextera sequencing library (Illumina Nextara DNA Sample Preparation Kit)
characteristics.characteristic: description
characteristics.text: Transduced cells (lentivirus)
characteristics.characteristic: genetic modification
characteristics.text: HEK293 with human ORFeome version 3.1
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: cell_culture_1
characteristics.characteristic: title