characteristics.text: 2012-01-11T17:00:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:35:51Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1094711
characteristics.characteristic: External Id
characteristics.text: SC
characteristics.characteristic: INSDC center name
characteristics.text: 2012-01-11T17:00:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:35:51Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS083918
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-858:CHRF
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: CHRF-288-11
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: CHRF 288-11 cells were cultured in RPMI-1640 (Sigma-Aldrich, Gillingham, UK, R0883), 10 % FBS (Invitrogen, Paisley, UK, 10100-147) and 200 uM L-glutamine, 100 U penicillin and 100 ug/ml streptomycin (Sigma-Aldrich, G1146). We performed FAIRE in the human megakaryocytic cell line CHRF-288-11. Cells were maintained in RPMI-1640 medium (Sigma-Aldrich, R0883), 20 % horse serum (heat- inactivated, Invitrogen, 26050-088), and 1 % L-glutamine-penicillin-streptomycin solution (Sigma-Aldrich, G1146), and grown at 37C, 5 % CO2, and 100% humidified atmosphere. FAIRE experiments were carried out as previously described. Twenty million cells were cross-linked with 1 % formaldehyde for 12 min and subjected to 12 sonication cycles (30 sec of high pulse, 30 sec of rest) using the Bioruptor UCD-200. A DNA amplification step was neglected. The sample was cleaned-up using the QIAGEN MinElute PCR Purification Kit (Qiagen, 28004). FAIRE DNA was processed following the Illumina paired-end library generation protocol.
characteristics.characteristic: description
characteristics.text: megakaryoblastic leukaemia
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_1001932
characteristics.characteristic: disease state
characteristics.text: CHRF
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: CHRF
characteristics.characteristic: title