characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-11-13T17:02:20Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:54:55Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2250475
characteristics.characteristic: External Id
characteristics.text: Laboratory of Molecular Medicine and Genomics, Department of Medicine and Surgery, University of Salerno, Baronissi, Italy.
characteristics.characteristic: INSDC center name
characteristics.text: 2014-11-13T17:02:20Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:54:55Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS371123
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2069:ZR75.1_wt
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: ZR75.1
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001262
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Human breast cancer ZR75.1 cells were used to produce stable clones expressing ER-beta tagged with 3xFlag-tag respectively at the N-term (ZR-flag-ER-beta). All were grown in Dulbeccos modified Eagles medium (DMEM), supplemented with 10% fetal bovine serum (FBS) (both from Invitrogen SpA, San Giuliano, Milanese, Italy), 100 U/ml penicillin, 100 mg/ml streptomycin, 250 ng/ml Amfotericin-B, 50 ug/ml G418 (normal growing condition). For G0-G1 synchronization cells were plated at 40% confluence in steroid-free medium (phenol red-free DMEM medium, with 5% fetal bovine serum pre-treated with dextran-coated charcoal and antibiotics) and maintained for 5 days without any additional scompound Cells were harvested in absence of estrogen stimuli Total RNA was extracted using TRIZOL reagent (Invitrogen) according to the manufacturer instructions. 1ug of total RNA was used in a library preparation according to the Illumina TruSeq small RNA sample preparation protocol rev. E (Illumina, USA).
characteristics.characteristic: description
characteristics.text: breast cancer
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000305
characteristics.characteristic: disease
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: ZR75.1_wt
characteristics.characteristic: title