characteristics.text: 2014-01-14T17:00:43Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:32:18Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2189755
characteristics.characteristic: External Id
characteristics.text: Institute for Molecular Biology and Tumor Research
characteristics.characteristic: INSDC center name
characteristics.text: 2014-01-14T17:00:43Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:32:18Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS317048
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1731:L3MBTL2 kr-flag
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HEK293
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001182
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: For stable expression of either 3xFLAG-L3MBTL2 or 3xFLAG-L3MBTL2K675/700R 1.4x10^6 HEK293 ncells having a stably integrated GAL4-responsive luciferase reporter (Stielow et al., 2008a) were transfected with 1 µg of linearized pN3-3xFLAG-L3MBTL2 or pN3-3xFLAG-L3MBTL2K675/700R, respectively. Forty-eight hours upon transfection cells were selected with 1 µg/ml puromycin and 1 µg/µl G-418. Single clones were isolated, expanded and L3MBTL2 expression was analyzed by western blotting. ChIP-Seq experiments were performed using the OneDay ChIP kit (Diagenode) in accordance to the manufacturer’s instructions. Library construction was done with the Illumina TRUSeq kit according maufacturers instructions.
characteristics.characteristic: description
characteristics.text: transfection
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000726
characteristics.characteristic: genetic modification
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: L3MBTL2 kr-flag
characteristics.characteristic: title