characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-10-01T17:02:38Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T20:55:51Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2797533
characteristics.characteristic: External Id
characteristics.text: Department of Cell and Molecular Biology, Karolinska Institutet
characteristics.characteristic: INSDC center name
characteristics.text: 2015-10-01T17:02:38Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T20:55:51Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS559144
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2983:P22
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: DDX4 FACS sorted single cell. FACS was performed on pooled ovarian cortical tissue from two individuals, 33 and 36 years old. Protocols: Single cells (n = 41) were randomly picked by mouth pipetting with a finely pulled glass capillary and placed directly into lysis buffer containing 1 uL of RNase inhibitor and 19 uL of 0.2% (vol/vol) Triton X-100 solution (Sigma-Aldrich, Germany). The 8-strip PCR tubes containing cells were immediately frozen on dry ice and kept at -80C for downstream applications. Regarding spike-ins: 0.1uL of a 4*10^-6 dilution of the ERCC RNA spike-in mix 1 (Ambion) was added to each sample. Picelli et al, Full-length RNA-seq from single cells using Smart-seq2, Nature Protocols, 2014
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: 21
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: animal ovary
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000992
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: OK
characteristics.characteristic: single cell well quality
characteristics.text: P22
characteristics.characteristic: title