characteristics.text: 2011-10-01T17:00:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-09T09:52:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1016547
characteristics.characteristic: External Id
characteristics.text: DUKE
characteristics.characteristic: INSDC center name
characteristics.text: 2011-10-01T17:00:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-09T09:52:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS009611
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-284:2ndrun_2nd
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: In order to establish single spore isolates sporophytes were surface sterilized and their spore content germinated in petri dishes (9 mm diameter) containing full strength Hoaglands medium (14h day/10h night, 40 mmol/m2/sec, 25 oC). After one week ten sporelings were transplanted to new separate Petri dishes in order to establish single spore isolates. Emerging protonemata (appr. 2 weeks old) were transferred to liquid culture (full strength Hoaglands solution) and two weeks later, contents of the liquid cultures were emptied into small pots (5 x 5 cm) filled with soil (1:1 mixture of potting soil and sand). Single spore isolates were kept under these conditions until gametophytes filled the pot (appr. 1.5-2 months). Plants were watered (ddH2O) from below. To generate sufficient material for RNA extraction and to correct for the inherent stochasticity of gene expression the single genetic individual was clonally replicated by dividing the mat into eight equal parts and each was transferred to a new pot. Four pots (referred to as sporophyte pots) were immediately brought to the greenhouse whereas the rest (referred to as gametophyte pots) were kept in a growth chamber and received water just from below to prevent self-fertilization (14h day/10h night, 40 mmol/m2/sec, 25 oC). When the first gametangia and/or young sporophytes appeared in the sporophyte pots (appr. after one month) the gametophyte pots were also brought to the greenhouse. Gametophyte and sporophyte pots were grown next to one other in the greenhouse for at least one month and were watered (ddH2O) for 10 minutes at 120 minute intervals by an automatic misting system. Sporophyte and gametophyte tissues were collected separately from each pot in parallel between 8-10 am. Premeiotic sporophytes with both swollen and unswollen capsules (sporangia) were clipped at their bases. Gametophyte tissues were obtained by clipping the top five millimeters from the gametophores. Tissues collected were immediately immersed into RNAlater solution (Ambion, Woodward, Austin, TX) and stored at -80 oC prior to RNA extraction (for appr. 2-3 weeks).
characteristics.characteristic: description
characteristics.text: Funaria hygrometrica
characteristics.characteristic: organism
characteristics.text: Funaria hygrometrica
characteristics.characteristic: organism
characteristics.text: gametophyte
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0030014
characteristics.characteristic: organism part
characteristics.text: Funaria hygrometrica
characteristics.characteristic: scientific_name