characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-01-20T17:31:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T19:25:54Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2696866
characteristics.characteristic: External Id
characteristics.text: Institute of Biochemistry and Biophysics, Polish Academy of Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2015-01-20T17:31:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T19:25:54Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS525445
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2807:Ler_h1.3_S_1
characteristics.characteristic: Submitter Id
characteristics.text: 5 week
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000034
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: Plants were sown in Jiffy expandable peat pots (diameter 27-30). In the third-leaf stage (16 days after sowing) six peat pots were buried in a single large plastic pot (4.5 dm3) filled with strong loamy sand:sand composition (7:2 v:v). In each large pot plants of: h1.3 and its sibling wild-type (WT) were placed in equal number. At that stage only one plant of equal size was left in each single peat pot. After two weeks of growth ( 25 degrees C, PFD 250 umol m-2 s-1 provided by HPS lamps 'Agro' (Philips, Brussels, Belgium), photoperiod 14/10, RH = 40%) treatment was conducted. Plants were grown for 17 days under light intensity 40 umol m-2 s-1 with field water contents in soil gradually decreased down to 20% FWC (water was controlled daily, as in control object). Leaves from 6-8 plants from one box were pooled into biological replicates and frozen in liquid nitrogen The samples were ground in liquid nitrogen using mortar and pestle. DNA was extracted by SDS lysis followed by Phenol-Chloroform DNA purification The DNA was bisulfite-converted with Zymo Research EZ DNA Methylation Kit according to manufacturer's recommendations. The libraries were prepared with Bioo Scientific NEXTflex DNA Sequencing Kit according to manufacturer's recommendations for paired-end libraries.
characteristics.characteristic: description
characteristics.text: flowering plant
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007016
characteristics.characteristic: developmental stage
characteristics.text: Ler
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005154
characteristics.characteristic: ecotype
characteristics.text: h1.3 mutant
characteristics.characteristic: genotype
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: Ler_h1.3_S_1
characteristics.characteristic: title