characteristics.text: 2014-01-14T17:00:44Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:35:52Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2163244
characteristics.characteristic: External Id
characteristics.text: Cancer Research UK Cambridge Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2014-01-14T17:00:44Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:35:52Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS319432
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1749:MDA-MB-453
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: MDA-MB-453
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001215
characteristics.characteristic: cell line
characteristics.text: epithelial
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000066
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: The LNCaP prostate cancer cell line and the ER-AR+ molecular apocrine breast cancer cell line, MDA-MB-453, were obtained from American Type Culture Collection (ATCC). Cells were grown in RPMI or DMEM media respectively, both supplemented with 10% Fetal Bovine Serum, 2 mM L-glutamine, 50U/ml penicillin and 50ug/ml streptomycin in 37oC incubator with 5% CO2. Cells were grown in full media, and harvested without further treatment. Cells were harvested 48hours after treatment. For all experiments, 1x10^8 cells were cross-linked with 1% formaldehyde as previously described (Schmidt et al, Methods 48(3) July 2009 doi:10.1016/j.ymeth.2009.03.001). ChIP-seq assays were performed as previously described (Schmidt 2009). Protein-bound DNA was immunoprecipitated with an antibody against androgen receptor (AR) (Santa Cruz, sc-816). Immunoprecipitated DNA was end-repaired, A-tailed and single-end Illumina sequencing adapter ligated before 18 cycles of PCR amplification. A 2% agarose gel was used to select 200-300 bp DNA fragments.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: MDA-MB-453
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001215
characteristics.characteristic: title