characteristics.text: 2014-01-23T17:02:40Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:35:37Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2157032
characteristics.characteristic: External Id
characteristics.text: University of Dublin, Trinity College
characteristics.characteristic: INSDC center name
characteristics.text: 2014-01-23T17:02:40Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:35:37Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS319653
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1746:Control 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Heterozygous matings of Splotch-delayed mice were harvested at E14.5 (TS23) Splotch-delayed homozogous mutant and control (homozygous wild-type and heterozygote) embryos were dissected and Humerus forelimb tissue was dissected at embronic day 14.5 (Theiler Stage 23) RNA was extracted following manufacturers guidlines ( SV. Total RNA Isolation system, Promega, UK) The DNase-treated RNA (3ug) was used to prepare RNA-Seq libraries with the TruSeq RNA Sample Prep kit. A total of six cDNA libraries were constructed, representing triplicate biological replicates for each group.
characteristics.characteristic: description
characteristics.text: Theiler stage 23
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004411
characteristics.characteristic: developmental stage
characteristics.text: Splotch-delayed (Pax3Spd/+)
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: humerus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000976
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Control 2
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001461
characteristics.characteristic: title