characteristics.text: 2012-12-31T17:01:41Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:47:02Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SRA-Webin
characteristics.characteristic: ENA-SUBMISSION-TOOL
characteristics.text: SAMEA2008520
characteristics.characteristic: External Id
characteristics.text: BCCS
characteristics.characteristic: INSDC center name
characteristics.text: 2012-12-31T17:01:41Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:47:02Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS148506
characteristics.characteristic: SRA accession
characteristics.text: Smc3
characteristics.characteristic: Submitter Id
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: characteristics = 1-7HB2 cells(Bartek et al. 1991) characteristics = Chromatin immunoprecipitation for SMC3 growth_protocol = HB2 cells (1-7HB2, a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7, (Bartek et al. 1991)) were cultured in DMEM supplemented with 0.2 mM L-glutamine, 100 units/ml penicillin, 100 mg/ml streptomycin, 10% FCS, 5 �g/ml hydroxycortisone and 10 �g/ml human insulin SMC3_description = polyclonal rabbit anti-SMC3 antibodies obtained from Jan-Michael Peters was used for ChIP as described in (Wendt, K.S., Yoshida, K., Itoh, T., Bando, M., Koch, B., Schirghuber, E., Tsutsumi, S., Nagae, G., Ishihara, K., Mishiro, T., Yahata, K., Imamoto, F., Aburatani, H., Nakao, M., Imamoto, N., Maeshima, K., Shirahige, K., and Peters, J.M. 2008. Cohesin mediates transcriptional insulation by CCCTC-binding factor. Nature 451(7180): 796-801.). SMC3_IgG_description = rabbit pre-immune serum was used for ChIP as described in (Wendt, K.S., Yoshida, K., Itoh, T., Bando, M., Koch, B., Schirghuber, E., Tsutsumi, S., Nagae, G., Ishihara, K., Mishiro, T., Yahata, K., Imamoto, F., Aburatani, H., Nakao, M., Imamoto, N., Maeshima, K., Shirahige, K., and Peters, J.M. 2008. Cohesin mediates transcriptional insulation by CCCTC-binding factor. Nature 451(7180): 796-801.).
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Smc3 Chip Seq sample
characteristics.characteristic: title