characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-06-24T17:01:42Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:08:16Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2336812
characteristics.characteristic: External Id
characteristics.text: University of Manchester
characteristics.characteristic: INSDC center name
characteristics.text: 2014-06-24T17:01:42Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:08:16Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS395007
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2239:Oct4i_0in_4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: E14Tg2a
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006275
characteristics.characteristic: cell line
characteristics.text: embryonic stem cell from blastocyst
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: ES cells were maintained in Ndiff N2B27 media in the presence (ES conditions) of CHIR99021 (3uM) and PD032590 (1uM). Control RNAi, Oct4 RNAi or Otx2 RNAi, in the presence (ES conditions) or absence (Differentiation, 12hr) of CHIR99021 (3uM) and PD032590 (1uM). RNA was isolated using RNeasy plus mini Kit (QIAGEN) followed by DNaseI treatment (Ambion, Turbo DNA-free) and re-purify by Agencourt RNA clean XP beads (Beckman coulter) then stored at -80C SENSE mRNA-seq library Prep kit (Lexogen)
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: Oct4i_0in_4
characteristics.characteristic: title