characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-06-19T17:01:49Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T18:27:04Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2604742
characteristics.characteristic: External Id
characteristics.text: University of Regensburg
characteristics.characteristic: INSDC center name
characteristics.text: 2014-06-19T17:01:49Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T18:27:04Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS481704
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1237:caRNA fraction3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: embryonal extract
characteristics.characteristic: cell type
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: Chromatin assembly and accessibility analysis were performed as described elsewhere (Becker and Wu, 1992; Rhodes and Laskey, 1989; Stewart et al., 1991) For RNA isolation, chromatin was fractionated in a linear sucrose gradient (15-40%; 12 h; 7500g; 4�C). Fractions containing the chromatin associated RNA were de-proteinized and treated with DNase I. Isolated RNA was enriched for fragments < 200 bp and strand-specific cDNA libraries were prepared using the ExactStart Small RNA Cloning Kit (Epicentre). Libraries included small coding as well as non-coding RNAs and fragments of longer primary transcripts. The cDNA was amplified with hybrid primers containing sequences required for the Illumina platform. PCR products were cleaned up with AMPure XP beads (Beckman Coulter).
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: caRNA fraction3
characteristics.characteristic: title