characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-03-01T17:02:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T20:24:07Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2771250
characteristics.characteristic: External Id
characteristics.text: Department of Molecular Neuroscience, UCL Institute of Neurology
characteristics.characteristic: INSDC center name
characteristics.text: 2015-03-01T17:02:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T20:24:07Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS543998
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2940:RNA_Seq_LUs24
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HEK293
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001182
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were not treated with siRNA (untreated), treated with anti-STAU1 siRNA (knockdown), or treated with anti-STAU1 siRNA and the STAU1 level was rescued by the rescue expression of siRNA-resistant STAU1 (rescue). Cells were subjected to Cycloheximide treatment before collection. RNA-Seq was performed in parallel with ribosome profiling. The protocol was followed to isolate RNA from the Cycloheximide teated cells and amplify cDNA library as described in the manuscript. Specific barcodes were used for each RT reaction as described in the manuscript. Random barcodes were also incoperated into cDNAs to distinguish between PCR duplication of cDNA products. The final products were obtained by PCR with Illumina paired-end sequencing primers: 5-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3; 5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: RNA_Seq_LUs24
characteristics.characteristic: title