characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-03-25T17:01:39Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T21:16:28Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2768219
characteristics.characteristic: External Id
characteristics.text: University of Zurich
characteristics.characteristic: INSDC center name
characteristics.text: 2015-03-25T17:01:39Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T21:16:28Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS542789
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2925:Oci-ly10
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Oci-ly10
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: The DLBCL cell lines used were SU-DHL4, SU-DHL6, SU-DHL10, SU-DHL16 and RC-K8 of GC DLBCL subtype and U-2932, Oci-Ly3, Oci-Ly10, SU-DHL2, SU-DHL5 and RIVA of ABC DLBCL as well as SU-DHL7 (unclassified). Cell lines were maintained at 37 degrees C, 5%CO2 in a humidified atmosphere in RPMI or IMDM (RIVA, Oci-Ly10) supplemented with 10% (Oci-Ly10, RIVA, SU-DHL2 and SU-DHL5) or 20% heat-inactivated FBS and antibiotics. CD19 positive B cells were isolated from buffy coats of healthy volunteer blood donors obtained from the blood donation centre Zurich (ZHBSD). The quality of the isolated RNA was determined with a Qubit® (1.0) Fluorometer (Life Technologies, California, USA) and a Bioanalyzer 2100 (Agilent, Waldbronn, Germany). Only those samples with a 260 nm/280 nm ratio between 1.8-2.1 and a 28S/18S ratio within 1.5-2 were further processed. The TruSeq RNA Sample Prep Kit v2 (Illumina, Inc, California, USA) was used in the succeeding steps. Briefly, total RNA samples (100-1000 ng) were poly A enriched and then reverse-transcribed into double-stranded cDNA. The cDNA samples was fragmented, end-repaired and polyadenylated before ligation of TruSeq adapters containing the index for multiplexing Fragments containing TruSeq adapters on both ends were selectively enriched with PCR. The quality and quantity of the enriched libraries were validated using Qubit® (1.0) Fluorometer and the Caliper GX LabChip® GX (Caliper Life Sciences, Inc., USA). The product is a smear with an average fragment size of approximately 260 bp. The libraries were normalized to 10nM in Tris-Cl 10 mM, pH8.5 with 0.1% Tween 20.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Oci-ly10
characteristics.characteristic: title