characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-11-13T17:02:20Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:54:04Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2259376
characteristics.characteristic: External Id
characteristics.text: European Molecular Biology Laboratory
characteristics.characteristic: INSDC center name
characteristics.text: 2014-11-13T17:02:20Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:54:04Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS373104
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2103:130814puf3_RIP_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: baker's yeast
characteristics.characteristic: common name
characteristics.text: Protocols: Grow single colony overnight in 5ml YPD at 30C unless the growth media is otherwise specified in sample annotations. Dilute in 50-100ml desired medium with starting OD600=0.1. Grow to OD600=1.0. Harvest cells for total RNA extraction. Flash frozen pellets of exponentially growing TAP tagged cells (OD600 0.5-0.8) were mechanically lysed with glass beads using a FastPrep24 agitator. An aliquot of each cell lysate was used as an input control. Cell lysates were subjected to immunoprecipitation with Dyna M280 sheep anti-rabbit IgG beads (Invitrogen) for 2h at 4??C. RNA-protein complex was released by TEV protease cleavage of the TAP-tag. The immunoprecipitated RNA was purified by phenol:chloroform:isoamyl alcohol extraction. In summary 10 ug of total RNA was fragmented, purified, retrotranscribed, end-repaired, ligated, amplified and finally size selected. Full protocol is describe in An efficient method for genome-wide polyadenylation site mapping and RNA quantification Wilkening et al., 2013
characteristics.characteristic: description
characteristics.text: puf3-TAP
characteristics.characteristic: genotype
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4932
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: scientific_name
characteristics.text: mating type alpha
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001270
characteristics.characteristic: sex
characteristics.text: BY4742 MAT alpha, his3-delta-1, leu2-delta-0, lys2-delta-0, ura3-delta-0, pep4-delta-::KANR
characteristics.characteristic: strain
characteristics.text: 130814puf3_RIP_1
characteristics.characteristic: title