characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-04-22T17:01:20Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T18:27:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2731823
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY
characteristics.characteristic: INSDC center name
characteristics.text: 2016-04-22T17:01:20Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T18:27:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS481847
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2663:MCF7 CTRL
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: MCF-7
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001203
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: MCF7 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with non-essential amino-acids (1%), penicillin and streptomycine (1%), gentamycin (0.1%), fungizone (0.1%) and 10% FCS. RNAs were purified from cultured cells using the High Pure RNA isolation kit (Roche). The integrity of RNA was checked with BioAnalyser 2100 (Agilent technologies) that indicated a RQI score >8 The libraries were prepared with Truseq mRNA Sample Prep kit (Illumina) from 1 microgram of total RNA following manufacturer's instructions. mRNAs were isolated by poly-A selection and fragmented (8 minutes at 94°). Fragmented mRNAs were used for reverse-transcription in the presence of Superscript II (Invitrogen) and random primers. After second strand synthesis, end-repair, A-tailing and purification, the double strand cDNA fragments were ligated to Truseq adapters containing the index sequences. Fifteen cycles of PCR in the presence of dedicated PCR primers and PCR master mix were applied to generate the final libraries.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: MCF7 CTRL
characteristics.characteristic: title