characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-11-11T17:02:10Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T21:36:49Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2755489
characteristics.characteristic: External Id
characteristics.text: Institute for Genetics University of Cologne
characteristics.characteristic: INSDC center name
characteristics.text: 2014-11-11T17:02:10Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T21:36:49Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS541369
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2907:TPI_PTC189_2_CGGA
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HeLa
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001185
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Standard lab conditions were used to grow the HeLa cells used for these experiments HeLa cells were grown over night in 6 cm plates and transiently transfected with 300 pmol siRNA using Lipofectamine RNAiMAX (Life Technologies). 24 h post transfection the cells were split 1:2 in 10 cm plates and the day after transfected again with 600 pmol siRNA. RNA samples were obtained from XRN1 knockdown HeLa cells transfected with the indicated reporter mRNA expressing plasmids (TPI-WT or -PTCs). Total RNA was extracted using Isol-RNA-Lysis Reagent. 3' fragments were linker ligated, reverse transcribed and specific PCR products were amplified with gene specific primers.
characteristics.characteristic: description
characteristics.text: TPI PTC189
characteristics.characteristic: fraction
characteristics.text: XRN1 siRNA-mediated knockdown
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: TPI_PTC189_2_CGGA
characteristics.characteristic: title