characteristics.text: 2013-03-21T17:02:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:17:21Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1713692
characteristics.characteristic: External Id
characteristics.text: EMBL-EBI
characteristics.characteristic: INSDC center name
characteristics.text: 2013-03-21T17:02:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:17:21Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS214127
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1499:TS50pg
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: A. thaliana total RNA was diluted using RNase-free water, and 50 pg were used for each cDNA library preparation using the SMARTer Ultra Low RNA Kit for Illumina Sequencing from Clontech according to manufacturers instructions. 24 cycles of PCR were used for library amplification, and after passing quality control, finalized cDNA libraries (6 ng - 20 ng) were fragmented with the Covaris S2 system as reported previously (Tang et al. 2010 2010 Nat Protoc 5(3): 516-535) using Covaris mircoTUBEs (#520045) and a volume of 130 ul for shearing (libraries were diluted to that volume using RNase-free water). After fragmentation, the volume was reduced to 44 ul using a SpeedVac concentrator and samples were subjected to standard Illumina library preparation using the NEBNext ChIP-Seq Sample Prep Master Mix Set 1 kit according to manufacturers instructions. Illumina PE adapters including custom-made multiplexing barcodes were ligated (amount of adapters was adjusted according to amount of input material), and Illumina PE primers (PE PCR Primer 1.0 and PE PCR Primer 2.0) were used for the PCR enrichment step (15 cycles) of the NEBNext protocol. The final purification step was performed using AMPure XP beads rather than columns and clusters were generated following the standard Illumina protocol.
characteristics.characteristic: description
characteristics.text: TS50pg
characteristics.characteristic: individual
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: TS50pg
characteristics.characteristic: title