characteristics.text: hepatocyte
characteristics.characteristic: CellType
characteristics.text: 2012-03-01T17:00:34Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:35:11Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1035477
characteristics.characteristic: External Id
characteristics.text: CRUK-CRI
characteristics.characteristic: INSDC center name
characteristics.text: 2012-03-01T17:00:34Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:35:11Z
characteristics.characteristic: INSDC last update
characteristics.text: killed
characteristics.characteristic: INSDC status
characteristics.text: Mus musculus
characteristics.characteristic: Organism
characteristics.text: liver
characteristics.characteristic: OrganismPart
characteristics.text: ERS088184
characteristics.characteristic: SRA accession
characteristics.text: C57BL/6xA/J
characteristics.characteristic: StrainOrLine
characteristics.text: E-MTAB-941:HNF6 ChIP 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Hepatocytes were prepared by direct perfusion of the liver with buffered salt solution followed by percent formaldehyde. After 10 minutes the tissue was removed and diced in 500 mM glycine buffer to neutralize the formaldehyde. After homogenization the hepatocytes were rinsed with PBS. The animal livers were crosslinked with formaldehyde treatment and chromatin fragmented to an average of 300 bp by sonication. Chromatin from an mass equivalent of 1/4 mouse liver was used for each ChIP experiment. Solexa libraries were prepared following the instructions of Illumina (Sample preparation for genomic DNA version 2.2) with the following modifications. The ChIP-enriched DNA and input DNA were not further fragmented. After end-repair and addition of an A base to the 3 prime ends, the adapters were ligated to the ends of the DNA Fragments using 2 ml of fourtyfold diluted Adapter oligo mix in a total reaction volume of 25 ml. Between these steps, the DNA was purified using the DNA Clean-and-Concentrator-5 kit (Zymo Research). Subsequently, the DNA was amplified by 18 cycles of PCR, purified with QIAquick PCR purification Kit, and eluted with 33.5 ml of 10 mM tris buffer at pH7.0. The PCR-product was sized fractionated on 2 percent agarose gel and a gel slice containing the 200-300 bp fragments was excised.
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: HNF6 ChIP 2
characteristics.characteristic: title