characteristics.text: 2012-08-01T17:00:53Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:37:53Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1486465
characteristics.characteristic: External Id
characteristics.text: The University of Edinburgh
characteristics.characteristic: INSDC center name
characteristics.text: 2012-08-01T17:00:53Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:37:53Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS099364
characteristics.characteristic: SRA accession
characteristics.text: BALB/c
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000602
characteristics.characteristic: StrainOrLine
characteristics.text: E-MTAB-995:BTG_2
characteristics.characteristic: Submitter Id
characteristics.text: 10 weeks
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000034
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: peritoneal macrophage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000581
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: BALBc and IL4Ra-/- mice were intra-peritoneally injected with 700ul thioglycollate (4%) or intra-peritoneally implanted with 4 B. malayi females and 1 male. Macrophages were harvested 3 days post injection or 21 days post-implantation. Peritoneal exudate cells were harvested by lavage and purified using flow cytometry based upon F4/80 expression. Purity in each sample was confirmed to be >90%. Cells were Qiazol (Qiagen) prior to RNA extraction. Isolation: F4/80+, SiglecF-, B220-, CD4-ve Total RNA was extracted using the miRNeasy kit (Qiagen) according to the manufacturers protocol. Quality was assessed on an Agilent bioanalyzer, all samples had RIN scores >8. Each library was composed of 0.66ug total RNA pooled from 3 mice. RNA-Seq libraries were prepared using the Illumina Paired-End library preparation kit according to the manufacturers instructions.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: BTG
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: BTG_2
characteristics.characteristic: title