characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-01-11T17:01:11Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T22:44:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3205965
characteristics.characteristic: External Id
characteristics.text: School of Agriculture, Policy and Development University of Reading, UK
characteristics.characteristic: INSDC center name
characteristics.text: 2017-01-11T17:01:11Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T22:44:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS638344
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3226:Leaf
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: coconut palm
characteristics.characteristic: common name
characteristics.text: Protocols: Developing leaves (Leaf) were sampled in vitro germinated embryo culture plant (Weerakoon et al 2002). In vitro cultures were maintained at 28 degree centigrade under dark conditions in a culture room. Total RNA was extracted using an RNeasy Plant Mini Kit (Qiagen, UK) using manufacturers protocol. cDNA libraries were constructed using SMART approach as described by Zhu et al. 2001 using the the oligonucleotides SMART Sfi1A : 5prime- AAG CAG TGG TAT CAA CGC AGA GTG GCC ATT ACG GCCrGrGrG-3', CDS-Sfi1B : AAG CAG TGG TAT CAA CGC AGA GTG GCC GAG GCG GCCd(T)20-3', SMART PCR primer : 5'-AAG CAG TGG TAT CAA CGC AGA GT- 3'. The amplified cDNA PCR products were purified using QIAquick PCR Purification Kit (QIAGEN, CA) and a final yield of 3 microgram was prepared for each tissue type.
characteristics.characteristic: description
characteristics.text: 8 month old in vitro germinated coconut plantlet
characteristics.characteristic: developmental stage
characteristics.text: Sri Lanka Tall variety
characteristics.characteristic: genotype
characteristics.text: Cocos nucifera
characteristics.characteristic: organism
characteristics.text: Cocos nucifera L.
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organism part
characteristics.text: Cocos nucifera
characteristics.characteristic: scientific_name
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: Leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: title