characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-01-30T17:02:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T20:33:37Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2785672
characteristics.characteristic: External Id
characteristics.text: Institute for Molecular Biology and Tumor Research
characteristics.characteristic: INSDC center name
characteristics.text: 2015-01-30T17:02:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T20:33:37Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS555362
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2970:Nf-ya_wtMEFs
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: mouse embryonic fibroblast cell
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004040
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: MEFs were kept in DMEM/Hams F-10 (1:1) (PAA/GE Healthcare) supplemented with 10% FCS (Fetal Bovine Serum Gold, PAA), 1% PenStrep (Sigma). DNA was immunoprecipitated using the respective antibody in accordance to the One-Day Chip kit (Diagenode) protocol. Four individual ChIPs were pooled and precipitated DNA was purified using Qiaquick columns (Qiagen). Five nanogram of precipitated DNA was used for library preparation using the Microplex libary preparation kit.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Nf-ya_wtMEFs
characteristics.characteristic: title