characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-12-04T17:04:45Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T18:24:28Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2603440
characteristics.characteristic: External Id
characteristics.text: CECAD, university of Cologne
characteristics.characteristic: INSDC center name
characteristics.text: 2014-12-04T17:04:45Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T18:24:28Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS481500
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2640:sample_14
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fission yeast
characteristics.characteristic: common name
characteristics.text: Protocols: Yeasts were prepared in liquid 50 ml cultures grown at 32 degree celsius. Cells were harvested at an Optical Density at 595 nm of 0.4-0.5 by filtration and snap frozen in liquid Nitrogen. Total RNA was isolated by hot-phenol extraction (Lyne et al, 2003), and RNA quality was assessed on a Bioanalyzer instrument (Agilent) [Lyne R, Burns G, Mata J, Penkett CJ, Rustici G, Chen D, Langford C, Vetrie D & Bahler J (2003) Whole-genome microarrays of fission yeast: characteristics, accuracy, reproducibility, and processing of array data. BMC Genomics 4: 27] Strand-specific RNA-seq libraries were created for SOLiD sequencing from poly(dT)-enriched RNA using the SOLiDTM Total RNA-Seq Kit (Applied Biosystems, LifeTechnologies). For poly(dT)-enrichment, three rounds of poly(dT) Sera-Mag magnetic bead purifications were carried out using 50 ?g of total RNA starting material and verified on a Bioanalyzer. 500 ng poly(A) enriched RNA was then fragmented to an average size of ~200 nt. SOLiD adaptors were hybridized and ligated, RT-PCR performed, and the cDNA purified by size selection on 6% TBE-urea gels. The 3 barcodes (1-48) were incorporated at the library amplification step using 16 PCR cycles and purified using SPRI-beads (Agencourt, Beckman Coulter). Library size distributions and concentrations were determined on a Bioanalyzer, samples pooled and put through emulsion PCR steps.
characteristics.characteristic: description
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4896
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: scientific_name
characteristics.text: 968
characteristics.characteristic: strain
characteristics.text: sample_14
characteristics.characteristic: title