characteristics.text: 2013-06-03T13:10:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:22:59Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1906711
characteristics.characteristic: External Id
characteristics.text: University of Edinburgh
characteristics.characteristic: INSDC center name
characteristics.text: 2013-06-03T13:10:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:22:59Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS243123
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1654:D6s4B5 3N+r2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: intermediate reprogramming population
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: MEFs and ES Cells were cultured in GMEM + 100Units LIF. Intermediate reprogramming samples were cultured in the presence of dox, VitC and Alk5i for 10 or 15 days. 20,000 to 200,000 cells from each sample were sorted by flow cytometry. Pelleted cells were resuspended in 500uL TRI Reagent (Invitrogen). RNA was extracted in the usual manner via phenol-chloroform extraction. RNA quality was verified using a Agilent Bioanalyzer. RNA was diluted to a concentration of 10ng/uL in 10mM TRIS ph7.6, 0.05% Tween-20 buffer. Library was constructed as published in Islam et al. Genome Research, 21, 1160-1167, doi:10.1101/gr.110882.110 (2011).
characteristics.characteristic: description
characteristics.text: D6s4B5 3N+
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: D6s4B5 3N+r2
characteristics.characteristic: title