characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-08-20T17:01:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:10:02Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2343157
characteristics.characteristic: External Id
characteristics.text: University of Oxford
characteristics.characteristic: INSDC center name
characteristics.text: 2014-08-20T17:01:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:10:02Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS400849
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2033:Rela-120m-R1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: M1 Macrophages
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000235
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: For the generation of M1 macrophages differentiated with GM-CSF, bone marrow of wild type C57Bl6 mice was cultured in RPMI-1640 medium (PAA Laboratories) supplemented with recombinant mouse GM-CSF (20 ng/ml; Preprotech). After 8 d, cells were washed with PBS and replated, then stimulated with LPS (100 ng/ml; Alexis Biochemicals). 50, 100 and 300 million GM-CSF derived M1 macrophages were used for each Pol II, RelA and IRF5 ChIP experiment, respectively. Cells were fixed for 10 minutes with 1 % formaldehyde, quenched with 125 mM of Tris pH 7.5 and washed with ice-cold PBS. Nuclear Each lysate was immunoprecipitated with 10 usc_20x_hg19_rCRSchrm.fa.bamg of the following antibodies: IRF5 (Abcam; ab21689), RelA (Santa Cruz; sc-372) and PolII (Santa Cruz; sc-899). ChIP was performed for each antibody as described previously (Ghisletti et al. 2010). ChIPped DNA w
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: wildtype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005543
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: Rela-120m-R1
characteristics.characteristic: title