characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-07-14T16:39:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:15:53Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2399442
characteristics.characteristic: External Id
characteristics.text: University of Science of Technology of China
characteristics.characteristic: INSDC center name
characteristics.text: 2014-07-14T16:39:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:15:53Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS421809
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2264:corona radiata cells
characteristics.characteristic: Submitter Id
characteristics.text: 29 hour
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000032
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: cell type
characteristics.text: corona radiata cells
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Briefly, the cumulus' oocyte complexes were retrieved 36 h after hCG treatment and washed in multiple dishes with Flushing medium (William A. Cook Australia Pty. Ltd, Queensland, Australia). The cumulus oophorus cells were collected in the fertilization medium (William A. Cook Australia Pty. Ltd, Queensland, Australia) by two disposable needles and two 1-ml plastic disposable syringes without hyaluronidase. To avoid the interfusion of corona radiata cells, the innermost layers of cumulus oophorus cells were not collected. On the other hand, the corona radiata cells were collected as described in our previous publication and other reports. The corona radiata cells separated from the oocyte by gentle pipetting with a 135 mm diameter stripper pipette and micromanipulator System. MII oocytes were used for ICSI procedure. The corona radiata cells and cumulus oophorus cells were pooled by centrifuging at 1500g for 8 min, and immediately frozen in liquid nitrogen until use All the five patients were stimulated with the standard long gonadotrophin-releasing hormone agonist (GnRHa, Diphereline; Ipsen Pharma. Biotech, Signes, France) protocol combined with the administration of recombinant FSH (Gonal-f, Merk Serono SA, Geneva, Switzerland). For oocyte retrieve, all patients underwent ovarian puncture (OPU) of follicles larger than 15 mm after 36 h of administration of 10 000 IU human chorionic gonadotrophin (hCG, LiZhu Pharma, ZhuHai, China). Only the cumulus'oocyte complexs with Metaphase II (MII) oocytes were included in this study. Total RNAs were extracted from the human CRCs and COCs using TRIzol reagents (Invitrogen). Library preparation and sequencing was performed at BGI-Shenzhen.
characteristics.characteristic: description
characteristics.text: pool cells from five patients
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: corona radiata cells
characteristics.characteristic: title