characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-01-20T17:31:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T19:25:54Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2696867
characteristics.characteristic: External Id
characteristics.text: Institute of Biochemistry and Biophysics, Polish Academy of Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2015-01-20T17:31:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T19:25:54Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS525446
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2807:Col_wt_1
characteristics.characteristic: Submitter Id
characteristics.text: 14 day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: plants were grown for 14 days on plates in medium containing half Murashige and Skoog (MS) salts (Sigma-Aldrich) and 1% (w/v) agar, pH 5.8 under long day conditions (16 h light/8 h dark) at 22 degrees C, with 70% humidity and illumination of 120 uM m-2 sec-1 12-15 seedlings were pooled into biological replicates and frozen in liquid nitrogen The samples were ground in liquid nitrogen using mortar and pestle. DNA was extracted by SDS lysis followed by Phenol-Chloroform DNA purification The DNA was bisulfite-converted with Zymo Research EZ DNA Methylation Kit according to manufacturer's recommendations. The libraries were prepared with Bioo Scientific NEXTflex DNA Sequencing Kit according to manufacturer's recommendations for paired-end libraries.
characteristics.characteristic: description
characteristics.text: seedling
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007131
characteristics.characteristic: developmental stage
characteristics.text: Col-0
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005148
characteristics.characteristic: ecotype
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: Col_wt_1
characteristics.characteristic: title