characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-02-07T17:01:22Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T23:01:35Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3182757
characteristics.characteristic: External Id
characteristics.text: University of science of technology of China
characteristics.characteristic: INSDC center name
characteristics.text: 2015-02-07T17:01:22Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T23:01:35Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS635606
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3087:Sample 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: normal tissue adjacent to tumour
characteristics.characteristic: clinical information
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Total RNA was extracted from ten pairs of fresh-frozen penile cancer and matched histologically normal penile tissues using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. RNA with good quality e.g. A260/A280>1.8 was used for further experiments. Meanwhile, the RNA integrity was evaluated by with DNA chip facilitated by Agilent 2100 BioAnalyzer (Agilent Technologies, Palo Alto, CA, USA). Two smRNA libraries were constructed using homogenized and pooled total RNAs of ten individuals for each group (normal and cancerous penile tissues). For each group, 10 microgram of total RNA was used for library construction with a Small RNA Sample Prep Kit (Illumina, USA) following the manufacturer’s instructions with minor modifications. Briefly, after 15% (w/v) denaturing PAGE (polyacrylamide gel electrophoresis), the 18- to 28-nt fraction of total RNA was excised, purified, and ligated to 3’ and 5’ RNA adaptors using T4 RNA ligase. The adaptor-ligated sRNAs were introduced to RT-PCR with 15 cycles of PCR amplification. The PCR products (approximately 90-bp, corresponding to sRNA + adaptors) were purified on 4% agarose gels to create the libraries.
characteristics.characteristic: description
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 2
characteristics.characteristic: title