characteristics.text: 2012-10-13T17:01:33Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:33:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1552718
characteristics.characteristic: External Id
characteristics.text: Pfizer
characteristics.characteristic: INSDC center name
characteristics.text: 2012-10-13T17:01:33Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:33:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS067014
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-818:hES thaw A
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: CyT49
characteristics.characteristic: cell line
characteristics.text: embryonic stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002322
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: CyT49 (Viacyte proprietary male hESC line, normal karyotype) was grown as a monolayer culture by their standard methods [K. DÕAmour, personal communication]. Briefly, cells were thawed at 10 x 10^6 cells per T175 flask and grown in DMEM/F12 media (Gibco), 10% Xenofree Knockout Serum Replacement (Gibco), 1x non-essential amino acids (Gibco), 1% Glutamax (Gibco), 1% penicillin/streptomycin plus 10ng/ml activin A and Heregulin _1 at 37¡C and 8% CO2. Prior to initiating cellular differentiation, formation of cellular aggregates was achieved by placing the single cell suspension of undifferentiated ESCs into a rotational culture in 6-well, low attachment tissue culture plates in ESC growth media. Plates were rotated at 95 rpm overnight. For initiation of differentiation, the aggregates were pooled and washed with PBS prior to resuspending in day 0 differentiation media. Cells were treated with ActivinA + Wnt3a for one day, ActivinA alone for one day, KGF+ITS+TGF-Inhibitor for 3 days, Cyclopamine+TTNPB+Noggin for 3 days, KGF+EGF+Noggin for 3 days. Samples were taken at days 0, 1, 2, 5, 8 and 11 10 x 10^6 d0 cells and 3000 aggregates (d1-11) were fixed in 11% formaldehyde for ChIP-seq as described http://www.genpathway.com/support/fixation.html.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: hES thaw A
characteristics.characteristic: title