characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-12-31T17:01:12Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:13:26Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2395806
characteristics.characteristic: External Id
characteristics.text: DKFZ
characteristics.characteristic: INSDC center name
characteristics.text: 2015-12-31T17:01:12Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:13:26Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS419977
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2360:0_1H6_input
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HL-60/S4
characteristics.characteristic: cell line
characteristics.text: undifferentiated myeloid leukemic cell
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: HL-60/S4 cells were cultured in RPMI-1640 medium with 10% heat-inactivated fetal calf serum and 1% penicillin/streptomycin. Cells were differentiated into granulocytes by addition of all-trans ratinoic acid and into macrophage by phorbol ester. Cells were fixed, lysed and incubated with primary antibody, either PL2-6 or 1H6, before they were fixed again and sonicated in Covaris micro-chambers. The harvested supernatant was incubated with Protein A/G agarose to bind the epichromatin-antibody complex, and the remainder (input) represents the total nuclear chromatin. The epichromatin (IP) was eluted from the complex. DNA was purified with QIAquick PCR Purification Kit (QIAGEN). ChIP-seq libraries were prepared with NEB Next Chip Seq DNA Sample Prep Kit according to the manufacturers protocol.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 0_1H6_input
characteristics.characteristic: title