characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-06-10T17:03:29Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:21:40Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2466857
characteristics.characteristic: External Id
characteristics.text: MRC National Institute for Medical Research
characteristics.characteristic: INSDC center name
characteristics.text: 2014-06-10T17:03:29Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:21:40Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS436614
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2499:KO_LPS_2_s17
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: B cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000236
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Splenic, live B220+ CD93- IgM+ CD23+ follicular B cells were sorted by flow cytometry and then either left unstimulated or stimulated with either anti-IgM or LPS or CD40L with IL-4 Unstimulated or in vitro for 6 h with either anti-IgM or LPS or CD40L with IL-4 Trizol RNA extraction followed by RNEasy Mini Kit clean up TruSeq RNA sample preparation kit (Illumina)
characteristics.characteristic: description
characteristics.text: gene knock out
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000506
characteristics.characteristic: genetic modification
characteristics.text: Themis2KO/KO
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: spleen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0001281
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: KO_LPS_2_s17
characteristics.characteristic: title