characteristics.text: 2013-12-01T17:01:07Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:08:42Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2039233
characteristics.characteristic: External Id
characteristics.text: Aarhus University, Department of Molecular Biology and Genetics
characteristics.characteristic: INSDC center name
characteristics.text: 2013-12-01T17:01:07Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:08:42Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS187994
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1355:833492001
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: pig
characteristics.characteristic: common name
characteristics.text: Protocols: At an average weight of approximately 30 kg the offspring was transferred to the finishing unit. Slaughtering was done when the largest pig in the pen reached 110 kg. Blood samples were collected from the sows and muscle, lung and liver tissue from the boar and offspring were sampled at slaughter. The RNA fragments were then copied into first strand cDNA using reverse transcriptase and random hexamer primers followed by second strand cDNA synthesis using DNA Polymerase I and RNaseH. The short cDNA fragments were finally prepared for sequencing on the Illumina Genome Analyzer using reagents provided in the Illumina Genomic DNA Sequencing Sample Prep Kit. The cDNA fragments were end-repaired using T4 DNA polymerase and Klenow DNA polymerase and a single adenine nucleotide base was added to the cDNA fragments by using 3prime to 5prime exonuclease. This was followed by ligation of Illumina adaptors, gel purification of fragments of approximately 200 bases in size and finally PCR enrichment for 15 cycles. The concentrations of the libraries were measured using Invitrogen Qubit fluorometer and the size and purity were determined using the Agilent DNA 1000 Kit on Agilent 2100 Bioanalyzer. The 89 libraries were diluted in buffer EB from QIAGEN to 10 nM, denaturated with 2 N NaOH to a final DNA concentration of 1.0 nM, diluted to 4 pM with pre-chilled hybridization buffer from Illumina and loaded in individual lanes into a 1.0 mm flowcell together with a single lane of a 2 pM PhiX control library from Illumina.
characteristics.characteristic: description
characteristics.text: Single boar founder family
characteristics.characteristic: family history
characteristics.text: 833492001
characteristics.characteristic: individual
characteristics.text: Sus scrofa
characteristics.characteristic: organism
characteristics.text: Sus scrofa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9823
characteristics.characteristic: organism
characteristics.text: longissimus dorsi
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001401
characteristics.characteristic: organism part
characteristics.text: Sus scrofa
characteristics.characteristic: scientific_name
characteristics.text: 833492001
characteristics.characteristic: title