characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-02-16T17:02:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T22:17:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3121478
characteristics.characteristic: External Id
characteristics.text: Instituto Gulbenkian de Ciencia
characteristics.characteristic: INSDC center name
characteristics.text: 2015-02-16T17:02:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T22:17:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS605628
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3104:Sample 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: NS5
characteristics.characteristic: cell line
characteristics.text: neural progenitor cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000047
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: NS5 cells were cultured as previously described (Conti et al. 2005), using laminin as a media supplement (20ug/mL), and plated at a density of 28,000/cm2 NS5 cells were infected with pBABE-Ascl1-ERT2-IRES-GFP-puro (NS5_Ascl1), followed by selection in complete medium containing 1ug/mL puromycin (Calbiochem), and collected 24 hours after induction with 4-Hydroxytamoxifen at a final concentration of 50nM NS cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde in phosphate buffered saline and then lysed, sonicated and immunoprecipitated, as previously described (Castro et al., 2011), with antibodies anti-H3K27Ac and anti-H3K4me1 (ab4729 and ab8895 from Abcam). DNA libraries were prepared from 10ng of immunoprecipitated DNA according to the standard Illumina ChIP-seq protocol.
characteristics.characteristic: description
characteristics.text: Ascl1-ERT2
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Ascl1-ERT2 expressed and Tamoxifen-activated
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Sample 2
characteristics.characteristic: title