characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-06-01T16:20:35Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T22:59:06Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3180060
characteristics.characteristic: External Id
characteristics.text: CSHL
characteristics.characteristic: INSDC center name
characteristics.text: 2015-06-01T16:20:35Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T22:59:06Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS632932
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3198:AB2.2 NPC total polyA
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: AB2.2
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002812
characteristics.characteristic: cell line
characteristics.text: neural progenitor cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000047
characteristics.characteristic: cell type
characteristics.text: whole cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/GO_0005623
characteristics.characteristic: cellular component
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: ESC colonies were maintained on gelatinized cell culture dishes, on a feeder layer of irradiated mouse embryonic fibroblasts in knock-out DMEM supplemented with 15 % fetal bovine serum, non-essential amino acids and 1,000 U/ml leukemia inhibiting factor. NPCs were maintained on gelatinized cell culture dishes in N2 expansion medium composed of 50:50 DMEM / F12: neurobasal medium, supplemented with 1x N2, 0.05x B-27, 50 ug /ml BSA (fraction V), 1ug / ml laminin, and 10 ng / ml each murine basic fibroblast growth factor and epidermal growth factor Total RNA was isolated using TRIzol reagent. For ASO knock-down studies, total RNA was collected using TRIzol and precipitated in the presence of 7.5 ug GlycoBlue (Ambion, Life Technologies). RNA integrity was assessed on the Agilent 2100 Bioanalyzer instrument and only RNA samples with integrity scores > 9.5 (median 10.0) were used. Stranded libraries for paired-end sequencing were prepared as described in Parkhomchuk, D., Borodina, T., Amstislavskiy, V., Banaru, M., Hallen, L., Krobitsch, S., Lehrach, H., and Soldatov, A. (2009). Transcriptome analysis by strand-specific sequencing of complementary DNA. Nucleic Acids Res. 37, e123.
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: AB2.2 NPC total polyA
characteristics.characteristic: title