characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-06-01T16:20:34Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T22:39:55Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3175754
characteristics.characteristic: External Id
characteristics.text: Institute for molecular and tumor biology Philipps University Marburg Germany
characteristics.characteristic: INSDC center name
characteristics.text: 2015-06-01T16:20:34Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T22:39:55Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS628702
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3167:TA053
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: tumor associated macrophages
characteristics.characteristic: cell type
characteristics.text: ascites ollected from 30 patients with high-grade serous ovarian carcinoma undergoing primary surgery at the University Hospital in Marburg
characteristics.characteristic: clinical history
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Ascites was collected from 30 patients with high-grade serous ovarian carcinoma undergoing primary surgery at the University Hospital in Marburg. Informed consent was obtained from all patients according to the protocols approved by the local ethical committe After Ficoll gradient centrifugation, tumor-associated macrophages (TAMs) were purified by CD14 MACS and lysed in Peqgold (TRIzol-like solution based on phenol and chloroform). RNA was digested with DNase (MN) and purified on a Qiagen Minelute column. Illumina-compatible libraries were made using the Epicentre Scriptseq human/mouse/rat low input Gold kit. Illumina-compatible libraries were made using the Epicentre Scriptseq human/mouse/rat low input Gold kit. Libraries were created using the Illumina Truseq mRNA kit used according to the manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: OC52TAM
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0003237
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: TA053
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005309
characteristics.characteristic: title