characteristics.text: 2013-10-30T17:01:20Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:30:17Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2080867
characteristics.characteristic: External Id
characteristics.text: European Bioinformatics Institute (EBI)
characteristics.characteristic: INSDC center name
characteristics.text: 2013-10-30T17:01:20Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:30:17Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS252031
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1706:cell_culture_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: S2
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001233
characteristics.characteristic: cell line
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: S2 cells were grown in Schneiders Media (Invitrogen) supplemented with 10% of heat-inactivated bovine fetal serum to a density of 1 million cells per ml. HITS-CLIP was performed as described in (Chi et al., 2009) with the following modifications: Nuclear extracts prepared from Drosophila S2 cells were used for immunoprecipitations. After high-salt washes, the 3'-ends of bound RNAs were de-phosphorylated first with PNK in MES-PNK buffer (25mM MES at pH 6.0, 50mM NaCl, 10mM MgCl2, 0.1% Tween20, 5mM DTT, NO ATP, 1U/uL PNK [NEB]) by incubating the beads at 37 degrees C for 10 minutes and then with CIP (NEB) before ligation of the 3'-RNA tag. The libraries were prepared following Illumina's TruSeq ChIP sample prep kit (http://www.illumina.com/products/truseq_chip_sample_prep_kit.ilmn)
characteristics.characteristic: description
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: embryonic cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002321
characteristics.characteristic: strain
characteristics.text: cell_culture_1
characteristics.characteristic: title