characteristics.text: 2012-08-28T17:00:46Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:55:35Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1487534
characteristics.characteristic: External Id
characteristics.text: EPFL
characteristics.characteristic: INSDC center name
characteristics.text: 2012-08-28T17:00:46Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:55:35Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS167406
characteristics.characteristic: SRA accession
characteristics.text: DGRP-362
characteristics.characteristic: StrainOrLine
characteristics.text: E-MTAB-1266:2_A2_new_JbGjyOVh54gg
characteristics.characteristic: Submitter Id
characteristics.text: 3 to 5 day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: All fly stocks were grown at 25 C and a 12h light-dark cycle on corn-meal fly medium. We collected virgin flies from three of the DGRP lines (lines 362, 517 and 765) during three days and we set up the following crosses: _ 362 x _ 517 (and reciprocal) and _ 517 x _ 765 (and reciprocal). We performed RNA-seq to assess transcript expression profiles of 3-5 day old F1 females. 25 flies per sample were frozen between 1-3 pm Total RNA was extracted using the combined Trizol/RNeasy (Qiagen, www1.Qiagen.com) protocol. RNA quality was measured using RNA Labchips and Bioanalyzer from Agilent Technologies (http://www.chem.agilent.com). RNA-seq libraries were prepared using the RNA-True seq kit (Illumina).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: 2_A2_new_JbGjyOVh54gg
characteristics.characteristic: title