characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2013-11-15T17:01:11Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:51:40Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2229610
characteristics.characteristic: External Id
characteristics.text: Hospital Clinic de Barcelona
characteristics.characteristic: INSDC center name
characteristics.text: 2013-11-15T17:01:11Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:51:40Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS363540
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1990:in vitro human pancreatic progenitor cells (PP3)
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: H9
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0003045
characteristics.characteristic: cell line
characteristics.text: embryonic stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002322
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: The differentiation protocol is described in Cho, C.H. et al. Inhibition of activin/nodal signalling is necessary for pancreatic differentiation of human pluripotent stem cells. Diabetologia 55, 3284-3295 (2012) In vitro human pancreatic progenitor cells were fixed, sonicated and processed for ChIP as described (van Arensbergen, J. et al. Genome Res 20:722, 2010) using 1-1.5ug of the following antibodies: rabbit anti-H3K4me1 (ab-8895), goat anti-Pdx1 (Chris Wright, BCBC AB2027), goat anti-FOXA2 (sc-6554), rabbit anti-GATA6 (sc-9055X), rabbit anti-HNF1B(sc-22840-X), rabbit anti-ONECUT1 (sc-13050). The immunoprecipitated DNA was modified for sequencing following the Illumina sequencing following the manufacturer protocol (Preparing Sample for ChIP Sequencing of DNA (11257047 Rev A)).
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: in vitro human pancreatic progenitor cells (PP3)
characteristics.characteristic: title