characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-04-25T17:00:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:28:04Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2474756
characteristics.characteristic: External Id
characteristics.text: The EMBL-European Bioinformatics Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2014-04-25T17:00:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:28:04Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS444134
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2512:Cell 75
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: primary cell culture of spleenocytes
characteristics.characteristic: cell line
characteristics.text: In vitro Th2 differentiation of CD4+ spleenocytes
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Murine in vitro Th2 differentiation using Naive (CD4+CD62L+) cells in presence of IL4 and plate coated anti-CD3 and anti-CD28 antibody lysis in Fluidigm C1 according to single cell mRNA library preparation manual “Using the C1TM Single-Cell Auto Prep System to Generate mRNA from Single Cells and Libraries for Sequencing” RT using SMARTer chemistry, PCR with Advantage Polymerase and library prep using Nextera XT with 8x12 barcoding, according to manual “Using the C1TM Single-Cell Auto Prep System to Generate mRNA from Single Cells and Libraries for Sequencing”
characteristics.characteristic: description
characteristics.text: Day 4.5 in vitro Th2 differentiation
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: Il13 EGFP mouse, male, 6 weeks old
characteristics.characteristic: strain
characteristics.text: Cell 75
characteristics.characteristic: title