characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-10-08T17:01:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:18:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2421749
characteristics.characteristic: External Id
characteristics.text: WTCHG
characteristics.characteristic: INSDC center name
characteristics.text: 2014-10-08T17:01:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:18:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS426833
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2424:Bcell_indiv1_input
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Blood from Leukocyte Cone was processed using a Ficoll separation method [Neron S, Thibault L, Dussault N, Cote G, Ducas E, Pineault N, Roy A. 2007. Characterization of mononuclear cells remaining in the leukoreduction system chambers of apheresis instruments after routine platelet collection: a new source of viable human blood cells. Transfusion 47(6): 1042-1049 ]. Buffy coat was isolated and washed in HBSS buffer. Buffy coat was subjected to purification via positive selection with CD19-coated beads (Miltenyi AUTOMACS) following manufacturers procedures. Crosslinked chromatin was purified for use as an estimate of background. Following manufacturers recommended procedures
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: Bcell_indiv1_input
characteristics.characteristic: title