characteristics.text: 2016-04-30T17:10:00Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:22:46Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2064929
characteristics.characteristic: External Id
characteristics.text: EMBL-EBI
characteristics.characteristic: INSDC center name
characteristics.text: 2016-04-30T17:10:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:22:46Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS241155
characteristics.characteristic: SRA accession
characteristics.text: E-SYBR-10:M28-4C
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: baker's yeast
characteristics.characteristic: common name
characteristics.text: Protocols: Montalcino Grape, Cavalieri et al 2000 (PMID: 11035792). Cells were grown in YPD liquid medium for genomic DNA extraction DNA was extracted from 10 raise to 9 pelleted cells with the Qiagen Genomic DNA Isolation Kit following the manufacturer's instructions. Libraries were constructed using Illumina TruSeq DNA Sample Prep standard protocol. Briefly, 5 ug of high molecular weight genomic DNA (gDNA) was fragmented by Covaris sonication device. Following sonication, DNA fragments were end-repaired and A-tailed. Adapters were then ligated via a 3 prime thymine overhang. Finally, ligated fragments were amplified by PCR (10 cycles). insert sizes were about 400-500 bp as evaluated in an Agilent DNA 1000 Analyzer Chip.
characteristics.characteristic: description
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4932
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: scientific_name
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: M28-4C
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002860
characteristics.characteristic: strain
characteristics.text: M28-4C
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002860
characteristics.characteristic: title