characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-10-10T17:01:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:47:48Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2221654
characteristics.characteristic: External Id
characteristics.text: Tongji University
characteristics.characteristic: INSDC center name
characteristics.text: 2014-10-10T17:01:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:47:48Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS359663
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1959:HEK293_BPA
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HEK293
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001182
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: The human embryonic kidney 293 cells (HEK 293) were cultured at 37C in 5% CO2 as adherent monolayer in Dulbecco modified Eagle medim(DMEM) (Hiclone) supplemented with L-glutamine and 10% fetal bovine serum (FBS) (Hiclone). BPA powder was dissolved in the dimethyl sulfoxide (DMSO) and added to culture medium. The final concentration of BPA and DMSO is 10-6 M and 10-3 M, respectively. For BPA treatment, cells were treated with 10-6 M BPA for 48h. Meanwhile, cells cultured in BPA-free medium were used as the control. Total RNA was extracted from each sample using Trizol reagent (Invitrogen) according to the manufacturers instructions. mRNA enrichment and library preparation were performed at BGI-Shenzhen (sequencing service provider).
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: HEK293_BPA
characteristics.characteristic: title