characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-01-19T17:03:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T20:46:28Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3110336
characteristics.characteristic: External Id
characteristics.text: The Roslin Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2015-01-19T17:03:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T20:46:28Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS577564
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3068:LEC_indigenous_infected
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: lung epithelial cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000082
characteristics.characteristic: cell type
characteristics.text: chicken
characteristics.characteristic: common name
characteristics.text: Protocols: Three groups comprising three indigenous, three hybrid and three commercial two day-old chicks were used in the experiment. These were used to obtain airway epithelial cells which were isolated from the trachea and lungs and cultured by the methods described by Zaffuto et al., (2008). The tracheal epithelial cells and lung epithelial cells from the three groups once adopted into culture were split into two groups each having three replicates. One group was infected with a Kenyan virulent strain of Newcastle disease virus with a multiplicity of infection (MOI) of 0.5 and the second group served as control. Both groups were incubated for 36 hours after which total RNA was obtained RNA was obtained after standard Trizol extraction Libraries were prepared as per manufacturer’s instructions for the SOLiD total RNAseq kit
characteristics.characteristic: description
characteristics.text: Newcastle Disease
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007395
characteristics.characteristic: disease
characteristics.text: Indigenous
characteristics.characteristic: ecotype
characteristics.text: Gallus gallus
characteristics.characteristic: organism
characteristics.text: Gallus gallus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9031
characteristics.characteristic: organism
characteristics.text: Gallus gallus
characteristics.characteristic: scientific_name
characteristics.text: LEC_indigenous_infected
characteristics.characteristic: title