characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-03-13T17:01:50Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T21:49:28Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3177872
characteristics.characteristic: External Id
characteristics.text: Shanghai Jiao Tong University
characteristics.characteristic: INSDC center name
characteristics.text: 2015-03-13T17:01:50Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T21:49:28Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS630804
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3188:WT
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fission yeast
characteristics.characteristic: common name
characteristics.text: Protocols: We used a wild-type Schizosaccharomyces pombe 972 h- strain for all experiments. The strain was exponentially grown at 32 degree C in rich medium supplemented with glucose, adenine, histidine, lecucine and uracil. Cells were harvested and resuspended with pre-cooled lysis buffer (10mM EDTA, 10 mM Tris-HCl pH 8.0 and 50 mM NaOAc), which were further treated with mechanical pulverization. The cell material was stored at -80 degree C. Total RNA was extracted with the TRIZOL reagent according to the manufacturer's instructions (Invitrogen, Carlsbad, CA) and isolated RNA was quantified by bioanalyzer 2100. The CAGE library was prepared as the method described (Valen et al., Genome Res 2009) with some modifications to work with Illumina GA II sequencer. Briefly, cDNA synthesis was conducted by using 50ug total RNA and Superscript II revese transcriptase (Life Technologies). The cap selected cDNA/RNA hybrids were treated with RNase ONE Ribonuclease (promega) and purified cDNA was ligated with 5 prime end linker containing Mme I site. After 2nd strand synthesis, dsDNA fragments were digested with Mme I followed by the ligation with 3 prime end linker. The resulting CAGE tags were amplified by PCR, which was subject to deep sequencing.
characteristics.characteristic: description
characteristics.text: Normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: growth condition
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4896
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: scientific_name
characteristics.text: WT
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001103
characteristics.characteristic: title