characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-10-22T17:01:00Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:28:46Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2475633
characteristics.characteristic: External Id
characteristics.text: Stowers Institute for Medical Research
characteristics.characteristic: INSDC center name
characteristics.text: 2014-10-22T17:01:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:28:46Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS445011
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2521:enok[2]_ovaries_1
characteristics.characteristic: Submitter Id
characteristics.text: 4-6 day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: Drosophila melanogaster was bred at Stowers Institute. The enok1 and enok2 mutant alleles originated from Bloomington Stock Center. Flies were grown on standard media at 25C unless stated otherwise. Larvae were heat shocked twice at 37C for 40 min, with 30 min break at 25C in between, for two consecutive days starting from the second day after hatching. Females were conditioned with wet yeast for 2-3 days before ovary dissection. Total RNA was isolated by Trizol, followed by DNase treatment. Libraries were poly-A selected and generated using the default directional Illumina protocol, TruSeq Adapters.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: EMS induced mutation
characteristics.characteristic: genetic modification
characteristics.text: hs-Flp / +; FRTG13, enok[2] / FRTG13, ovo[D1-18]
characteristics.characteristic: genotype
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: stages 5-14 egg chambers
characteristics.characteristic: organism part
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: yw/+
characteristics.characteristic: strain
characteristics.text: enok[2]_ovaries_1
characteristics.characteristic: title