characteristics.text: GSC:MIMARKS:2.1
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2011-07-05T11:13:58Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: GSC, MIxS, MIMARKS
characteristics.characteristic: ENA-KEYWORD
characteristics.text: 2018-03-09T09:53:42Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: GSC:MIMARKS:2.1:compliant
characteristics.characteristic: ENA-STANDARD
characteristics.text: SAMEA1706553
characteristics.characteristic: External Id
characteristics.text: UH
characteristics.characteristic: INSDC center name
characteristics.text: 2011-07-05T11:13:58Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-09T09:53:42Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS040659
characteristics.characteristic: SRA accession
characteristics.text: ContVeg_week3
characteristics.characteristic: Submitter Id
characteristics.text: BioNumerics Assembler
characteristics.characteristic: assembly
characteristics.text: ENA
characteristics.characteristic: broker name
characteristics.text: 2005
characteristics.characteristic: collection date
characteristics.text: sieving of entire greenhouse pot content
characteristics.characteristic: composite design/sieving (if any)
characteristics.text: terrestrial biome, anthropogenic habitat, oil contaminated soil
characteristics.characteristic: description
characteristics.text: terrestrial biome
characteristics.characteristic: environment (biome)
characteristics.text: anthropogenic habitat
characteristics.characteristic: environment (feature)
characteristics.text: oil contaminated soil
characteristics.characteristic: environment (material)
characteristics.text: soil
characteristics.characteristic: environmental package
characteristics.text: ContVeg and Cont
characteristics.characteristic: experimental factor
characteristics.text: 20 m
characteristics.characteristic: geographic location (altitude/elevation)
characteristics.text: Finland
characteristics.characteristic: geographic location (country)
characteristics.text: 0-20 cm
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000015
characteristics.characteristic: geographic location (depth)
characteristics.text: N 60.22931829 E 25.02038598
characteristics.characteristic: geographic location (latitudeandlongitude)
characteristics.text: miens-survey
characteristics.characteristic: investigation type
characteristics.text: LH-PCR for peak size 519-522 bp
characteristics.characteristic: library screening strategy
characteristics.text: 96
characteristics.characteristic: library size
characteristics.text: pCR 2.1-TOPO
characteristics.characteristic: library vector
characteristics.text: Phusion proofreading polymerase
characteristics.characteristic: nucleic acid amplification
characteristics.text: FastDNA SPIN Kit for Soil, Qbiogene
characteristics.characteristic: nucleic acid extraction
characteristics.text: soil metagenome
characteristics.characteristic: organism
characteristics.text: fD1:AGAGTTTGATCCTGGCTCAG;PRUN518r:ATTACCGCGGCTGCTGG
characteristics.characteristic: pcr primers
characteristics.text: 1.0 g/kg soil dw
characteristics.characteristic: petroleum hydrocarbon
characteristics.text: destructive sampling
characteristics.characteristic: sample collection device or method
characteristics.text: template composite of 6 replicate DNA extractions
characteristics.characteristic: sample material processing
characteristics.text: soil metagenome
characteristics.characteristic: scientific_name
characteristics.text: Manual and BioNumerics Assembler
characteristics.characteristic: sequence quality check
characteristics.text: Sanger, once both directions with the vector primers
characteristics.characteristic: sequencing method
characteristics.text: 16S rRNA
characteristics.characteristic: target gene