characteristics.text: 2012-10-27T17:01:55Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:58:00Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1557280
characteristics.characteristic: External Id
characteristics.text: School of Biological Sciences, University of Bristol
characteristics.characteristic: INSDC center name
characteristics.text: 2012-10-27T17:01:55Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:58:00Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS172622
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1277:ZeroHoursRead
characteristics.characteristic: Submitter Id
characteristics.text: ECACC
characteristics.characteristic: biosourceprovider
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HeLa
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001185
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: HeLa cells were obtained from ECACC and grown in SILAC labelled DMEM with 10% v/v SILAC dialysed Foetal Calf Serum (Dundee Cell Products) for at least 5 population doublings. Cells were infected with wild type adenovirus serotype 5 at a multiplicity of infection of 30 Cells were re-suspended in 0.5ml 0.1% Triton X-100 to lyse the cytoplasm, nuclei were spun down and the cytoplasmic fraction was extracted with Trizol to obtain a total cytoplasmic RNA. Trizol extracted RNA was extracted again using RNAeasy (Qiagen)
characteristics.characteristic: description
characteristics.text: adenovirus infected
characteristics.characteristic: disease state
characteristics.text: ZeroHoursRead
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: ZeroHoursRead
characteristics.characteristic: title