characteristics.text: 2013-07-18T17:00:55Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:27:31Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2073060
characteristics.characteristic: External Id
characteristics.text: University Freiburg
characteristics.characteristic: INSDC center name
characteristics.text: 2013-07-18T17:00:55Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:27:31Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS248038
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1669:MSL2 iCLIP replicate 3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: clone 8
characteristics.characteristic: cell line
characteristics.text: wing disc derived
characteristics.characteristic: cell type
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: Clone 8 cells, expressing roX1 and roX2, were grown in M3 + 2% FCS + 5 ug/ml insulin + 2.5% fly extract at 25C. We have isolated nuclei, and used nuclear extracts in all experiments. We used Clone8 cells and antibodies against MLE (rat1) and MSL2 (d300, Santa Cruz). One 150mm dish of ~80% confluent cells is used per immunoprecipitation; 6uL from each antibody and 100uL slurry of Protein G (for MLE) or protein A (MSL2) Dynal beads (Invitrogen). The low-RNaseI (Ambion) concentration used for library-preparation was 0.5U/mL. Biological triplicates were tagged with different barcodes and mixed together after reverse-transcription.
characteristics.characteristic: description
characteristics.text: 3rd instar larva
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002682
characteristics.characteristic: developmental stage
characteristics.text: CME W1 cl.8+
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005743
characteristics.characteristic: genotype
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: MSL2 iCLIP replicate 3
characteristics.characteristic: title