characteristics.text: 2013-07-10T17:01:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:32:52Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2159074
characteristics.characteristic: External Id
characteristics.text: Institut Pasteur de Montevideo
characteristics.characteristic: INSDC center name
characteristics.text: 2013-07-10T17:01:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:32:52Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS316933
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1366:starting_sample1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: mesenchymal stem cell
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Stem cells were obtained from adipose tissue from obese human donors ( two males and one female, ages: 41, 52, 23) . All samples were isolated, collected after informed consent had been obtained. hASCs were isolated, cultured and characterized as following. Briefly, 100 ml of adipose tissue was washed with sterile phosphate-buffered saline (PBS) (Gibco Invitrogen). A one-step digestion by 1 mg/ml collagenase type I (Invitrogen) was performed for 30 mins at 37 degrees C during permanent shaking and was followed by filtration through first a 100- and then 40-um mesh filter (BD FALCON, BD Biosciences Discovery Labware, Bedford, MA, USA).
characteristics.characteristic: description
characteristics.text: undifferentiated
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000621
characteristics.characteristic: developmental stage
characteristics.text: patient61
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 3 day
characteristics.characteristic: time
characteristics.text: starting_sample1
characteristics.characteristic: title
characteristics.text: no adipocyte differentiation induction
characteristics.characteristic: treatment