characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-07-24T17:01:24Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T20:35:46Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2821765
characteristics.characteristic: External Id
characteristics.text: The Medical Research Council Cancer Unit, Hutchison/MRC Research Centre
characteristics.characteristic: INSDC center name
characteristics.text: 2015-07-24T17:01:24Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T20:35:46Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS568966
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3021:PRPF8 KD 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: CAL51
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005358
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Human Cal51 breast carcinoma cells were treated with either control siRNA or PRPF8 siRNA and harvested 54 hours post-transfection for total RNA. 4 experimental replicates for PRPF8 siRNA and 3 experimental replicates for Control siRNA are provided. Total RNA was extracted using Qiagen RNeasy kit according to manufacturers protocol. Illumina's TruSeq RNA-seq kit was used to prepare libraries for sequencing according to manufacturers protocol.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: PRPF8 KD 1
characteristics.characteristic: title