characteristics.text: 2012-01-09T14:41:02Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:35:14Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1094720
characteristics.characteristic: External Id
characteristics.text: SC
characteristics.characteristic: INSDC center name
characteristics.text: 2012-01-09T14:41:02Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:35:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS084018
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-918:lane1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: megakaryocyte
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000556
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: MKs were obtained by culture of CD34+ hematopoietic progenitor cells. In short, cord blood was collected with informed consent and CD34+ cells were prepared by magnetic bead selection. Purified cells were cultured (1x105 cells/ml) for 10 days in serum-free medium supplemented with human thrombopoietin and interleukin-1beta to differentiate into MKs.By day 10 more than 90% of cells stained positive for CD41 positive and negative for CD34. Total RNA was obtained by the Trizol method. Polyadenylated RNA was purified from this by two rounds of magnetic Oligo dT25 selection (Dynabeads Oligo (dT)25, Invitrogen) in the presence of RNase inhibitor (New England Biolabs, Hitchin, UK), combined with DNase treatment (DNA-free, Ambion, Invitrogen). The mRNA was fragmented using RNA fragmentation reagent (Ambion, Invitrogen) and precipitated with added glycogen (Ambion, Invitrogen), then cDNA produced by Superscript Double Stranded cDNA synthesis kit (Invitrogen), replacing oligo-dT primer with random primer. Thereafter, library preparation was as per mRNA-sequencing kit (Illumina, Little Chesterford, UK), starting with end repair.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: cord blood
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0012168
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: lane1
characteristics.characteristic: title