characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-02-10T17:00:48Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:52:50Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2242732
characteristics.characteristic: External Id
characteristics.text: The Nuffield Department of Medicine, University of Oxford, Oxford, UK
characteristics.characteristic: INSDC center name
characteristics.text: 2014-02-10T17:00:48Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:52:50Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS368733
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1994:cell_culture_Hypoxia_HIF-1a_siRNA_rep2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: MCF-7
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001203
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: MCF-7 cells were cultured in Dulbecco's modified Eagles medium DMEM (Invitrogen). Media were supplemented with and 10% Fetal Bovine Serum (FBS) (Sigma-Aldrich). Cells were incubated for 24 hours in an In vivo2 Hypoxia Work Station (Ruskin Technologies) in an atmosphere of either normoxia (21% oxygen) or hypoxia (1% oxygen). In addtion, cells were treated with scramble control, HIF-1a and/or HIF-2a siRNAs as describes in paper and cultured in hypoxia for 24 hours. Total RNA was prepared using the mirVana miRNA Isolation Kit (Ambion) and treated with DNaseI (TURBO DNA-free, Ambion). Ribosomal RNAs were depleted using RiboMinus Locked Nucleic Acid probes (Invitrogen). Poly A selected RNA-seq libraries were papered according to TruSeq RNA Sample Prep Kit (Illumina), directional PolyA selected RNA libraries were prepared using the ScriptSeq v2 RNA-Seq kit (Epicentre) and ribosomal depleted strand specific total RNA-seq libraries were prepared according to optimised protocol described in the paper.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: cell_culture_Hypoxia_HIF-1a_siRNA_rep2
characteristics.characteristic: title