characteristics.text: 2013-07-17T17:01:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:21:53Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2062611
characteristics.characteristic: External Id
characteristics.text: Biomedical Center, University of Iceland
characteristics.characteristic: INSDC center name
characteristics.text: 2013-07-17T17:01:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:21:53Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS233522
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1599:Transfection_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: P19EC
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002718
characteristics.characteristic: cell line
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: P19 embryonal carcinoma cells were cultured in DMEM with 10% fetal calf serum, The mouse P19ECs were obtained from ATCC and were cultured in DMEM, 10% fetal 655 calf serum, 2mM L-glutamine (Gibco) and 50 ug/ml penicillin/streptomycin (Gibco) P19ECs were co-transfected with TRE-Tight BLIMP1-EGFP, and and pCAG-tTA-IP using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. The cells were harvested and crosslinked with 1% formaldehyde 24 hours after transfection. Phenol Chloroform extraction Performed ChIP according to: Magnusdottir, E. et al. Epidermal terminal differentiation depends on B lymphocyte912 induced maturation protein-1. Proceedings of the National Academy of Sciences of the United States of America 104, 14988-93 (2007). Libraries were generated using Illumina ChIPseq DNA Sample 665 Preparation Kit, (cat. No 1003473)
characteristics.characteristic: description
characteristics.text: epiblast
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002532
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: Transfection_2
characteristics.characteristic: title