characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-05-05T17:07:19Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:08:38Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2729862
characteristics.characteristic: External Id
characteristics.text: EMBL Genome Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2016-05-05T17:07:19Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:08:38Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS396227
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2235:RNAseq_Fly_male_Msl1_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: S2
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001233
characteristics.characteristic: cell line
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were seeded in Nunc T-25 flasks at a concentration of 2.5 million cells in serum free Gibco Sneider 2.5 million cells were treated with 40 µg MSL1 dsRNA (approx 500bp) and harvested after 4 days of treatment. RNA was extracted with ALL prep DNA/RNA Mini Kit QIAGEN according to the manufacturer instructions. Poly-A RNA isolation, RNA fragmentation, and complementary DNA (cDNA) synthesis protocols were performed as in Yoon and Brem (2010). The cDNA was processed for DNA library preparation according to Parkhomchuk et al. (2009). In summary, the protocol includes RNA fragmentation, first-strand synthesis, and second-strand synthesis using deoxyribo- nucleotide triphosphates (dNTPs) and deoxyuridine triphosphates (dUTPs). End repair, A-tailing, and ligation were then performed as well as size selection for fragments of 300-350 bp. The second strand was cleaved by hydrolysis of uracil in the dsDNA. The resulting strand-specific cDNA was amplified before sequencing.
characteristics.characteristic: description
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: RNAseq_Fly_male_Msl1_1
characteristics.characteristic: title