characteristics.text: 2013-02-21T17:02:27Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:38:19Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1710140
characteristics.characteristic: External Id
characteristics.text: Institute for Molecular Biology and Tumor Research
characteristics.characteristic: INSDC center name
characteristics.text: 2013-02-21T17:02:27Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:38:19Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS099178
characteristics.characteristic: SRA accession
characteristics.text: C57BL/6N
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004472
characteristics.characteristic: StrainOrLine
characteristics.text: E-MTAB-994:MEF_deltaSp2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: mouse embryonic fibroblasts
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004040
characteristics.characteristic: cell line
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: MEFs were maintained at 37 degrees celcius in 50 % DMEM High Glucose and 50 % Ham's F10 Medium (PAA) supplemented with 10% fetal bovine serum (PAA) and 1X Penicillin and Streptomycin (PAA). ChIP-qPCR experiments were performed essentially as described previously (Stielow et al., 2008, see below) using the OneDay ChIP kit (Diagenode) according to the manufacturer's instructions. Briefly, MEFs and HEK293 cells were incubated with 1% formaldehyde for 10 min at room temperature followed by quenching with 150 mM glycine for 5 min. After sonification, 100 microgram of chromatin was used for single immunoprecipitations using 2 microgram of self-made Sp2 antibody (Baur et al., 2010). Ten immunoprecipitations were pooled for ChIP-seq and purified by the QIAquick PCR Purification kit (Qiagen). 10 ng of inmunoprecipitated DNA fragments were subjected to paired-end sequencing on an Illumina IIx Genome Ananlyzer. Stielow, B., et al. (2008). SUMO-modified Sp3 represses transcription by provoking local heterochromatic gene silencing. EMBO Rep 9, 899-906.
characteristics.characteristic: description
characteristics.text: deltaSp2
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: MEF_deltaSp2
characteristics.characteristic: title