characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2013-10-01T17:01:24Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:41:44Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2175889
characteristics.characteristic: External Id
characteristics.text: Dept of Clinical and Experimental Medicine, Linksping University, Sweden
characteristics.characteristic: INSDC center name
characteristics.text: 2013-10-01T17:01:24Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:41:44Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS343983
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1846:3A
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: platelet
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000233
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: The local Regional Ethical Review Board (Link_ping, Sweden) granted an ethical permission for this study. Unstimulated non-irradiated apheresis platelets were collected from two healthy human male and one healthy female blood donor. Platelets were collected by COBE Spectra system (Gambro BCT) and were used on the same collection day. Residual leukocytes were depleted with anti-CD45 conjugated Dynabeads, according to the manufacturers recommendations (Pan Leukocyte; Invitrogen, Carlsbad, CA). The platelet suspension, with a volume of 30 mL and a platelet count of 1.4 * 10exp9 cells/mL, was centrifuged at 800 g for 8 min and the supernatant was discarded. The platelet pellet was processed for leukocyte depletion, as recommended by the supplier of Pan Leukocyte reagent (Invitrogen, Carlsbad, CA). The leukocyte removal was performed at room temperature. Approximately 70 _ 75% of the original platelets were recovered after the leukocyte depletion. To investigate the level of leukocyte contamination, we determined the level of CD45 (PTPRC) transcript by qPCR using TaqMan Gene Expression Assay for this gene according to the recommendations of the supplier (Assay ID: Hs00894713_m1; Applied Bioystems, Carlsbad, CA, USA). For isolation of total RNA, we employed the miRVana RNA Extraction Kit as recommended by the supplier (Life Technologies). Ribosomal RNA was depleted with Ribo-Zero (Epicentre, Madison, WI, USA) and strand specific barcoded RNA-sequencing libraries were prepared using ScriptSeq v2 (Epicentre) according to manufacturers instructions.
characteristics.characteristic: description
characteristics.text: RNA Extract S3
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: 3A
characteristics.characteristic: title