characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2021-02-19T04:08:52Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T19:14:59Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2700100
characteristics.characteristic: External Id
characteristics.text: Vanderbilt University
characteristics.characteristic: INSDC center name
characteristics.text: 2021-02-19T04:08:52Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T19:14:59Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS527834
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2816:E15.5 Ngn3^GFP/GFP embryos 1
characteristics.characteristic: Submitter Id
characteristics.text: embryonic day 15.5
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002566
characteristics.characteristic: age measurement datum
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: pool of 6 E15.5 Ngn3^GFP/GFP embryos (4 litters) Protocols: Embryos were isolated from mice; the presence of vaginal plug at noon was considered as E0.5. Embryos obtained from heterozygous interbreeding were genotyped by fluorescence microscopy or were genotyped using PCR. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating.
characteristics.characteristic: description
characteristics.text: embryo stage
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007725
characteristics.characteristic: life cycle stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: B6.129S-Neurog3tm1(EGFP)Khk/Mmcd mouse strain
characteristics.characteristic: strain
characteristics.text: E15.5 Ngn3^GFP/GFP embryos 1
characteristics.characteristic: title