characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-05-22T17:38:28Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:42:17Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2547936
characteristics.characteristic: External Id
characteristics.text: Institut Pasteur
characteristics.characteristic: INSDC center name
characteristics.text: 2015-05-22T17:38:28Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:42:17Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS462746
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2595:Anopheles gambie (replicate 2)
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: African malaria mosquito
characteristics.characteristic: common name
characteristics.text: Protocols: The Anopheles gambiae Ngousso strain was originally initiated with mosquitoes collected in Cameroon in January 2006, and belongs to the M molecular and Forest chromosomal forms (Harris C, Lambrechts L, Rousset F, Abate L, Nsango SE, Fontenille D, Morlais I, Cohuet A. (2010). Polymorphisms in Anopheles gambiae immune genes associated with natural resistance to Plasmodium falciparum. PloS Pathog 6, e1001112.). Mosquito were reared under standard conditions as previously described (Mitri C, Jacques JC, Thiery I, Riehle MM, Xu J, Bischoff E, Morlais I, Nsango SE, Vernick KD, Bourgouin C. (2009). Fine pathogen discrimination within the APL1 gene family protects Anopheles gambiae against human and rodent malaria species. PloS Pathog 5, e1000576. Same as TREATMENT but uninfected 12 mosquitoes per pool, in each of two replicates, were collected 3 days after bloodmeal infection with ONN and homogenized in TriReagent (Sigma). Small RNAs fraction (<200 nt) were extracted using Nucleospin miRNA (Machery Nagel) using the Trizol protocol and submitted to Bioanalyser for quality assessment. Briefly, the 10- to 30-nt RNA fragments were purified from small RNA fraction on a 15% urea PAGE (Biorad). Small RNA library were prepared using TruSeq Small RNA Library reagents (Illumina).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: uninfected
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001460
characteristics.characteristic: infect
characteristics.text: Anopheles gambiae
characteristics.characteristic: organism
characteristics.text: Anopheles gambiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7165
characteristics.characteristic: organism
characteristics.text: uninfected
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001460
characteristics.characteristic: phenotype
characteristics.text: 3 days after bloodmeal
characteristics.characteristic: sampling time
characteristics.text: Anopheles gambiae
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: Ngousso
characteristics.characteristic: strain
characteristics.text: Anopheles gambie (replicate 2)
characteristics.characteristic: title