characteristics.text: 2014-04-09T17:00:53Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:16:27Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2050436
characteristics.characteristic: External Id
characteristics.text: Laboratory of Molecular Medicine and Genomics, University of Salerno
characteristics.characteristic: INSDC center name
characteristics.text: 2014-04-09T17:00:53Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:16:27Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS212675
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1495:MCF7_wt
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: MCF-7
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001203
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Human breast cancer MCF-7 wt were used to produce stable clones expressing ER-beta tagged with TAP-tag respectively at the C-term(Ct-ER-beta ) as previously described. All were grown in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% fetal bovine serum (FBS) (both from Invitrogen SpA, San Giuliano, Milanese, Italy), 100 U/ml penicillin, 100 mg/ml streptomycin, 250 ng/ml Amfotericin-B, 50 ug/ml G418 (normal growing condition) For G0-G1 synchronization cells were plated at 40% confluence in steroid-free medium (phenol red-free DMEM medium, with 5% fetal bovine serum pre-treated with dextran-coated charcoal and antibiotics) and maintained for 5 days without any additional scompound Cells were harvested in absence of estrogen stimuli Determination of miRNA expression profiles was performed by next generation sequencing (NGS) with sequencing-by-synthesis technology, since this allows a dynamic range of detection (from very low to highly abundant RNAs) and to measure relatively limited differences in expression between samples. 7.5ug of total RNA was used in a library preparation according to the Illumina TruSeq small RNA sample preparation protocol (Illumina, USA).
characteristics.characteristic: description
characteristics.text: breast cancer
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000305
characteristics.characteristic: disease state
characteristics.text: wt
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001103
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: MCF7_wt
characteristics.characteristic: title