characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-10-07T17:00:57Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:12:01Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2360972
characteristics.characteristic: External Id
characteristics.text: UCL Cancer Institute, University College London, London, WC1E 6BT, United Kingdom
characteristics.characteristic: INSDC center name
characteristics.text: 2014-10-07T17:00:57Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:12:01Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS405998
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2316:RNAseq-wildtype
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Kc167
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005819
characteristics.characteristic: cell line
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: Kc167 and S2R+ cells (obtained from the Drosophila Genomics Resource Centre [DGRC]) were grown in Schneiders Insect Cell Medium (GIBCO) supplemented with 10% FBS (Sigma) and 1% penicillin/streptomycin (Thermoscientific). Cell lines were kept at 25C. They were grown to approximately 70% confluence prior to treatment. For RNA-seq experiments total RNA was obtained from approximately 5 x 10^6 cells per sample using the Qiagen RNeasy mini kit. mRNA was then extracted using the Dynabeads mRNA Purification Kit (Life Technologies). 50-250 ng of mRNA was used to generate an mRNA library following manufacturers instructions (NEBnext mRNA Library Prep Master Mix E6110S). cDNA fragments of 250-350 bp were selected by agarose gel extraction and amplified with 12 cycles of PCR. Resulting libraries were assessed for concentration and size using the Qubit fluorometer and the Agilent2100 Bioanalyzer.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: RNAseq-wildtype
characteristics.characteristic: title