characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2013-10-22T17:01:33Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:52:07Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2225940
characteristics.characteristic: External Id
characteristics.text: EBC
characteristics.characteristic: INSDC center name
characteristics.text: 2013-10-22T17:01:33Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:52:07Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS362653
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1898:sample1_polyA
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Tissue and total RNA samples from two fetal frontal cortex tissues, 38 weeks female (Sample 1) and 23/24 weeks male (Sample 2) were purchased from Capital Biosciences. The commercial adult frontal lobe polyA+ RNA sample with two rounds of polyA+ selection was acquired from Clontech (Catalogue number: 636165). Cytoplasmic RNA, nuclear RNA, total RNA and PolyA+ RNA was extracted from each of the samples. A novel protocol for cytoplasmic and nuclear RNA extraction was developed, based on the Norgen purification kit. The SOLiD RNA-seq protocol was used to prepare RNA libraries
characteristics.characteristic: description
characteristics.text: 1
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: sample1_polyA
characteristics.characteristic: title