characteristics.text: 2016-06-15T17:03:10Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:27:14Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2072293
characteristics.characteristic: External Id
characteristics.text: University of Manchester
characteristics.characteristic: INSDC center name
characteristics.text: 2016-06-15T17:03:10Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:27:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS249770
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1674:pMad 3 to 3.5 hr PI exp1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: Embryos were collected from yw adults as in Saunders et al. 2013. Saunders et al. Extensive polymerase pausing during Drosophila axes patterning enables high-level and pliable transcription. 2013. Genes and Development. Published online May 15, 2013. http://dx.doi.org/10.1101/gad. 215459.113. Embryo ChIP was performed as described in Sandmann et al. 2006, except that 5% formaldehyde was used in the crosslinking solution. For PI samples, the guinea pig pre-immune serum was used instead of pMad antibody. Samples were sonicated for 30 min using a Bioruptor with 9 sec on, 1 min off cycles, on high-energy settings. Two independent biological replicates were performed for each ChIP at each time point. Sandmann T, Jakobsen JS, Furlong EE. ChIP-on-chip protocol for genome-wide analysis of transcription factor binding in Drosophila melanogaster embryos. 2006. Nature Protocols 1:2839-2855.
characteristics.characteristic: description
characteristics.text: embryo stage
characteristics.characteristic: developmental stage
characteristics.text: 3 to 3.5 hour
characteristics.characteristic: gestational age
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: whole embryo
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000922
characteristics.characteristic: organism part
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: mixed sex
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: pMad 3 to 3.5 hr PI exp1
characteristics.characteristic: title