characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-10-01T17:02:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:09:11Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2342538
characteristics.characteristic: External Id
characteristics.text: University of Pennsylvania
characteristics.characteristic: INSDC center name
characteristics.text: 2015-10-01T17:02:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:09:11Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS400223
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2266:P4 MIP-GFP mice 3
characteristics.characteristic: Submitter Id
characteristics.text: postnatal day 4
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: insulin-expressing immature beta cell
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Postnatal pancreata were partially dissociated. Islets were hand picked and further dissociated into single cells for FACS-based beta cell purification. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacture's instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008).
characteristics.characteristic: description
characteristics.text: juvenile stage
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: B6.Cg-Tg(Ins1-EGFP)1Hara/J mouse strain
characteristics.characteristic: strain
characteristics.text: P4 MIP-GFP mice 3
characteristics.characteristic: title