characteristics.text: 2013-07-24T17:00:43Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:28:39Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2075302
characteristics.characteristic: External Id
characteristics.text: Signaling and Functional Genomics, DKFZ
characteristics.characteristic: INSDC center name
characteristics.text: 2013-07-24T17:00:43Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:28:39Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS250812
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1613:5E
characteristics.characteristic: Submitter Id
characteristics.text: 21 day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Evi floxed mice were crossed with K14-Cre mice to delete Evi gene in epithelial cells. Skin of 21 day old mice was collected from mutants and wildtype animals. After tissue digestion RNA from epidermal sheets was prepared. Three fully independent biological replicates where sampled. RNA was extracted from epidermal sheets of three wildtype and Evi-LOF mice. The poly(A)+ fraction was isolated from each of the six samples with the Dynabeads mRNA Purification Kit (Invitrogen). For each sample, a library for sequencing with a SOLiD4 sequencer was prepared. random primers with a hexamer adapter sequence were annealed to the 3' end of the mRNA and the first DNA strand synthesized. Following RNA digestion, the DNA was purified using Agencourt RNAClean XP Beads. Random primers were annealed to the DNA for second strand synthesis. Agencourt Ampure XP Bead selection was used to purify and size select the double stranded DNA. The library was amplified and barcoded.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: gene knock out
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000506
characteristics.characteristic: genetic modification
characteristics.text: Evi floxed knockout
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: epidermis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001003
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: 5E
characteristics.characteristic: title