characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2013-11-18T17:01:14Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:36:52Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2150453
characteristics.characteristic: External Id
characteristics.text: Plant Pathology Group, Integrative Biology Zurich (IBZ), ETH Zurich
characteristics.characteristic: INSDC center name
characteristics.text: 2013-11-18T17:01:14Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:36:52Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS323959
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1726:7.I.72.3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: apple
characteristics.characteristic: common name
characteristics.text: Protocols: Plant material comprised young Malus x domestica (Borkh.) cv. Golden Delicious saplings grafted on M9T337 rootstock. Plants were kept in active growth with a 16h/8h (day/night) photoperiod and a minimum of 65.5 umol m-2 s-1 light intensity provided with white fluorescent lamps (Philips Master TL-D 36W/830) and fertilized once with Obstdunger 12:8:16:2:0.2 (N:P:K:Mg:B; OBA-Lanze, Hauert AG, Switzerland) one week prior the start of the experiment. Plants were grown under greenhouse condition at 20 +/- degrees C and 70% RH until 10 to 15 leaves stadium. Rape-seed oil (Maag AG, Switzerland) mixed with Alaxon 50 (Maag AG, Switzerland) and two weeks later Vertimec (Maag AG, Switzerland) have been used as chemical treatment before starting the experiment to keep plants free of pest-insects. For inoculation, conidia of Venturia inaequalis (Cooke) single spore isolate no. 1639 were used.Inoculation, with a spore suspension of 5 x 105 spores ml-1, was performed at 17 +/- 2 degrees C and >98% RH. . Half of the plants were challenged with the apple scab pathogen and half were mock inoculated. After the treatment (scab or mock inoculation), plants were grown at 17 +/- 2 degrees C and 70% RH until sampling. The first sets of samples have been collected 72 hours post inoculation (hpi). The second sets of samples have been collected at 96 hpi. Samples have been collected from leaf 1 (L1) and leaf 7 (L7) by removing the leaf tip (< 100 mg) of each leaf in three biological replicates for inoculated and not inoculated shoots, 12 samples for each time points (72 and 96 hpi), 24 in total. Total RNA was extracted using SV Total RNA Isolation System (Promega Corporation, USA) with column purification Complementary DNA (cDNA) libraries were constructed, starting from 1 ug of total RNA, at the Functional Genomic Center Zurich (FGCZ) following the TruSeq RNA Sample preparation protocol v. 2 instructions (Low Throughput protocol, Illumina, Inc.).
characteristics.characteristic: description
characteristics.text: Leaf 7 (old leaf: seventh leaf position starting from the top of the shoot)
characteristics.characteristic: developmental stage
characteristics.text: inoculated with Venturia inaequalis
characteristics.characteristic: infect
characteristics.text: Malus domestica
characteristics.characteristic: organism
characteristics.text: Malus domestica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3750
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organism part
characteristics.text: Malus domestica
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: 17 +/- 2 degree celsius
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000027
characteristics.characteristic: temperature
characteristics.text: 72 hour
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000032
characteristics.characteristic: time post inoculation
characteristics.text: 7.I.72.3
characteristics.characteristic: title