characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-08-17T17:01:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:42:04Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2547646
characteristics.characteristic: External Id
characteristics.text: Systems Biology Ireland, Conway Institute, University College Dublin
characteristics.characteristic: INSDC center name
characteristics.text: 2016-08-17T17:01:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:42:04Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS462459
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2580:cell_culture_7
characteristics.characteristic: Submitter Id
characteristics.text: 24 hour
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000032
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HEK293
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001182
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: HEK293 cells were cultured in DMEM containing 10% FCS and Pen/strep. HEK293 cells were transfected with Control or REST RNA interfeerence using lipofectmain for 24 hours. Cells were exposed to normoxic (21% oxygen) or hypoxic (1% oxygen) pre-conditioned media for 24 hours after transfection. RNA was isolated using QIAgen QIAquick columns. For library construction the illumina TruSeq v2 mRNA kit was utilized after poly(A)+ RNA was enriched from total RNA samples. Libraries were visualized by Bioanalyzer (Agilent) using the High Sensitivity Chip. Libraries were quantified for pooling and sequencing using Kapa Biosystems qPCR quantitation kit.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: cell_culture_7
characteristics.characteristic: title