characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-05-21T14:57:04Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T19:53:00Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2639693
characteristics.characteristic: External Id
characteristics.text: EBI
characteristics.characteristic: INSDC center name
characteristics.text: 2015-05-21T14:57:04Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T19:53:00Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS505777
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2733:Athaliana2_NSS
characteristics.characteristic: Submitter Id
characteristics.text: 6 weeks
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000034
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: A. thaliana plant 2 RNA. Small RNA libraries were generated by Illumina TruSeq v2 NSS treatment Protocols: Total RNA was extracted from N. benthamiana tissue based on the White and Kaper protocol (/White, J.L., and Kaper, J.M. (1989). A simple method for detection of viral satellite RNAs in small plant tissue samples. J. Virol. Methods 23, 83 to 93). Total RNA from the all other organisms (A. thaliana, M. musculus, C. elegans, D. melanogaster) were extracted by TRIzol reagent (Life Technolgies). To make the small RNA libraries, this total RNA was used directly for Illumina TruSeq small RNA cloning protocol (RS-200-9002DOC) (NSS= non size selected). Or was subject to this protocol after small RNA size selection, by extraction of the 18-30nt band from a denaturing (formamide) polyacrylamide gel (FSS) or after small RNA size selection by extraction of the 18-30nt band from a fully denaturing formaldehyde polyacrylamide gel (FDFSS - described in detail by Harris et al,. in prep).
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: inflorescence
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009049
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: Col-0
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005148
characteristics.characteristic: strain
characteristics.text: Athaliana2_NSS
characteristics.characteristic: title