characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-02-01T17:01:18Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:18:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2421724
characteristics.characteristic: External Id
characteristics.text: LRI CRUK
characteristics.characteristic: INSDC center name
characteristics.text: 2015-02-01T17:01:18Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:18:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS426808
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2414:CHiC_ESC_rep2_t2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: embryonic stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002322
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Fetal livers were dissected from embryos at day 14.5 (E14.5) of development. Fetal liver cells were filtered through a cell strainer (70uM) and directly fixed in formaldehyde. Hi-C was performed following (Lieberman-Aiden et al., Science, 2009, DOI: 10.1126/science.1181369 ) with modifications detailed in Schoenfelder et al.). For CHiC experiments promoter interactions were enriched using a biotynilated RNA bait library as described. For random ligation (RL) libraries the crosslink was reversed before the ligation step in the HiC protocol, the dilution step was omitted, and the library was enriched the same way as described for CHiC.
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: 129S4/SvJae
characteristics.characteristic: strain
characteristics.text: CHiC_ESC_rep2_t2
characteristics.characteristic: title