characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-02-25T17:03:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:50:35Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2225914
characteristics.characteristic: External Id
characteristics.text: VGH-YM Genome Research Center
characteristics.characteristic: INSDC center name
characteristics.text: 2015-02-25T17:03:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:50:35Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS362599
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1961:MCF7 cells
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: MCF-7
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001203
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: MCF7 cells were cultured in RPMI1640 medium (GIBCO/BRL) supplemented with 10% (v/v) fetal bovine serum (GIBCO/BRL), 2.0 g/L sodium bicarbonate, and were incubated in a humidified 37C incubator with 5% CO2. MCF7 cells were grown to 85% confluence in 6 cm tissue culture dish. Each 6 cm dish was rinsed twice with 1X PBS. Total RNA was extracted using TRIreagent (Invitrogen) protocol. The integrity of the RNA extract was checked by 1.2% (w/v) agarose gel electrophoresis and the concentration of RNA was estimated by ultraviolet spectrophotometry. The normal breast tissue mRNA were purchased from Origene (Catalog No.: CR559104 and CR561898). The sequencing library for mRNA-seq was prepared using TruSeq RNA Sample Preparation Kit (Illumina Inc., USA) as per manufacturers instruction. Briefly, total RNA with RIN (RNA integrity number) greater than 7.5 was subjected for poly-A mRNA isolation using poly-T oligo-attached magnetic beads. The poly-A mRNA was fragmented and first-strand cDNA was synthesized using random hexamers following by second-strand cDNA synthesis, end repair, addition of a single A base and adapter ligation. The adapter-ligated cDNA library was sized-selected by agarose gel and amplified by PCR.
characteristics.characteristic: description
characteristics.text: breast adenocarcinoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000304
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: MCF7 cells
characteristics.characteristic: title