characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2013-10-15T17:00:42Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:38:13Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2168571
characteristics.characteristic: External Id
characteristics.text: TRON - Translational Oncology at the Johannes Gutenberg-University Mainz
characteristics.characteristic: INSDC center name
characteristics.text: 2013-10-15T17:00:42Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:38:13Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS337175
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1820:Ctrl-GMP
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Non-induced compound R26/AE controls For obtaining cells for the following RNA-Seq experiments, 4 x 10^4 L-K+S+IL7Ra-CD34+FcgRII/III+ cells were preparatively sorted using a FACSAria cell sorter (BD). RNA was extracted with the Pico Pure RNA Extraction Kit (Arcturus) according to the manufacturers instructions. The resulting RNA was analysed using an Agilent Bioanalyzer. RNA samples were amplified with Nugen`s Ovation RNA-Seq System and quantified by Qubit measurement. The TruSeq DNA Sample PrepKit (Illumina) was used and the resulting cDNAs were fragmented using AFA technology (Covaris). Subsequently, cDNA fragments were end repaired and 3' adenylated. Illuminas TruSeq adaptors with integrated indices were ligated and the resultant fragments size selected using 2% E-Gels (Invitrogen). Enrichment was done by linear PCR and the resulting fragments were purified using Agencourt AMPure XP magnetic beads.
characteristics.characteristic: description
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: bone marrow
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: Ctrl-GMP
characteristics.characteristic: title