characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-07-24T17:01:24Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T19:55:40Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2665142
characteristics.characteristic: External Id
characteristics.text: EBI
characteristics.characteristic: INSDC center name
characteristics.text: 2015-07-24T17:01:24Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T19:55:40Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS513153
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2787:1hr_Dox_A_TTAGGC_L001_R1_001
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: SH-SY5Y/6TR(EU)/pTrex-Dest-30/MYCN (SY5Y-MYCN)
characteristics.characteristic: cell line
characteristics.text: neuroblast
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000337
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Un-induced SY5Y-MYCN cells and cells treated with 1ug/ml Doxycycline (Sigma) to induce MYCN overexpression, for 1h, 4h 24h were used. For the time-course treatment start times were staggered so all samples were lysed simultaneously. Total mRNA was extracted using TRI Reagent (Sigma-Aldrich) according to manufacturer’s protocol, and DNA was digested with DNA-free Kit (Applied Biosystems). miRNA libraries (SY5Y-MYCN; un-induced, 1h, 4h and 24h MYCN induction) were prepared using TruSeq small RNA sample preparation kit (Illumina).
characteristics.characteristic: description
characteristics.text: MYCN Tet-ON
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: doxycycline-mediated overexpression of MYCN
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 1 hour
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000032
characteristics.characteristic: time
characteristics.text: 1hr_Dox_A_TTAGGC_L001_R1_001
characteristics.characteristic: title