characteristics.text: 2011-12-15T17:00:21Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:33:40Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1035328
characteristics.characteristic: External Id
characteristics.text: SC
characteristics.characteristic: INSDC center name
characteristics.text: 2011-12-15T17:00:21Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:33:40Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS078444
characteristics.characteristic: SRA accession
characteristics.text: JB371
characteristics.characteristic: StrainOrLine
characteristics.text: E-MTAB-5:run33_s4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fission yeast
characteristics.characteristic: common name
characteristics.text: Protocols: All cDNA samples used for Illumina sequencing were prepared by first treating ~1 mg of total RNA for 30 min with amplification grade RNase-free DNase (Invitrogen), according to the manufacturer’s protocols. PolyA enriched RNA was then prepared using an oligo(dT) selection kit (Oligotex Direct mRNA MiniKit, Qiagen). The resulting poly(A)-enriched RNA was then converted to double-stranded cDNA using a cDNA synthesis kit (Superscript choice system for cDNA synthesis, Invitrogen) , primed by an oligo(dT) primer, according to the manufacturer’s protocols. Random-primed RNA from rapidly proliferating cells was treated with an enzyme that specifically degrades non-capped RNA molecules (mRNA-only™ Eukaryotic mRNA isolation kit, Epicentre Biotechnologies) according to the manufacturer’s protocols. The resulting cDNA was then converted to double-stranded cDNA as above, except that the reaction was primed with random hexamers. RNA samples from the pooled meiotic timepoints were subjected to IVT amplification after undergoing a poly(A) enrichment step as described above. RNA from the oxidative stress condition and prp2 mutant was treated in the same way as the rapidly proliferating samples with oligo(dT)-primed cDNA synthesis after mRNA enrichment.
characteristics.characteristic: description
characteristics.text: pat1-114/pat1-114 ade6-M210/ade6-M216 h+/h+
characteristics.characteristic: genotype
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4896
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: scientific_name
characteristics.text: mating_type_h_plus/plus
characteristics.characteristic: sex
characteristics.text: run33_s4
characteristics.characteristic: title