characteristics.text: 2012-09-28T17:01:38Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:46:01Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1523628
characteristics.characteristic: External Id
characteristics.text: Cambridge Research Institute Genomics Core Facility
characteristics.characteristic: INSDC center name
characteristics.text: 2012-09-28T17:01:38Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:46:01Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS134261
characteristics.characteristic: SRA accession
characteristics.text: CAST/EiJ x C57BL/6J
characteristics.characteristic: StrainOrLine
characteristics.text: E-MTAB-1091:do930
characteristics.characteristic: Submitter Id
characteristics.text: 4 to 6 month
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000035
characteristics.characteristic: age
characteristics.text: frozen_sample
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: bio source type
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: All mice used in this study were housed and handled in accordance with the Animals (Scientific Procedures) Act 1986. Mice were housed and cared for in the Cambridge Research Institute Biological Resource Unit with a twelve hour light/dark cycle, and were provided with chow food from Lab Diet plus water ad libitum. Mice were sacrificed by cervical dislocation at 4-6 months of age, between the hours of 9:30am and 11:30am. The liver was then cut and the mouse perfused with PBS by injection into the heart. Subsequently, the liver was removed and the gall bladder and hepatic vein cut away. A sample of approximately 50mg-100mg was taken from a single liver lobe and frozen on liquid nitrogen for storage at -80C until use. For each liver sample, total RNA was extracted using the RNeasy Mini kit (Qiagen 74104) as per manufacturer’s instructions. Before further processing, each total RNA sample was treated to digest contaminating DNA. From each sample, polyadenylated mRNA was enriched from the total RNA using the PolyATract mRNA isolation system.
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: do930
characteristics.characteristic: title