characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2017-06-12T17:01:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T21:52:20Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3172289
characteristics.characteristic: External Id
characteristics.text: University of Manchester
characteristics.characteristic: INSDC center name
characteristics.text: 2017-06-12T17:01:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T21:52:20Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS626532
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3157:E14.5
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Palatal shelves from 5 embryos were dissected from E13.5 mouse embryos and pooled for RNA extraction. The same was done for E14.5 mouse embryos. All mice were housed with free access to food and water under specific pathogen free conditions at the University of Manchester. Temperature 20-22 °C, Humidity 40-60%, Specified mouse pathogen free conditions, 12 hours light/dark cycle. Nutrients Rat and Mouse Standard Diet no.1 expanded (Ban Kingman). All experiments were performed under regulations of The UK Home Office Scientific Procedures Act (1986). BALB/c mouse strain (Charles River Ltd, UK) were used for the experiment. Embryos were obtained after matings, the morning of the vaginal plug being considered embryonic day (E) 0.5. RNA was isolated mouse palatal shelves using the Qiagen RNeasy kit. SOLiDTM Total RNA-Seq Kit (PN 4445374)
characteristics.characteristic: description
characteristics.text: embryonic day 14.5
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002565
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: palate
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0012316
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: E14.5
characteristics.characteristic: title