characteristics.text: 2013-09-01T17:01:52Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:26:48Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2071682
characteristics.characteristic: External Id
characteristics.text: School of Life Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2013-09-01T17:01:52Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:26:48Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS245270
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1671:Group VL - Pool YL4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: honey bee
characteristics.characteristic: common name
characteristics.text: Protocols: Bees were developing in a brood frame Varroa-free colony for 3 days after hatching from egg. Then entire the brood frame with bee larvae was transferred to a Varroa-infested colony with high levels of Deformed wing virus (DWV). Both Varroa-free colony and Varroa-infested colony were kept in outdoor flight cages, bees were fed with pollen sourced in Varroa-free region and with sugar syrup. Larvae of worker honeybee, Apis mellifra (day 3 of bee development), which were hatched from the eggs laid in a colony free from Varroa destructor mites and harbouring low levels of Deformed wing virus (DWV), were either left to develop in the same Varroa-free hive before sampling at pupal stage (day 15) (EnvironmentalHistory-NoTransfer), or were transferred to a Varroa-infested colony with high levels of DWV in bees and the mites (EnvironmentalHistory-TransferToVarroaColony). All transferred larvae were receiving the DWV strains present in this Varroa-infested colony with the food delivered by the nurse bees until capping on day 9. About half of these larvae were capped with Varroa mite and were subjected to the mite piercing and feeding on their haemolymph for 5 days during pupal development until sampling on day 14 (GrowthCondition -MiteFeeding), the rest of the pupae developed without the mite (GrowthCondition- NoMite). Exposure to the mite piercing and feeding resulted in about 1000-fold increase of the DWV levels in about 80% of the mite-exposed pupae (DiseaseState-HighDWV). In the rest of mite-exposed pupae the levels of DWV remained relatively low (DiseaseState-LowMidDWV) and were similar to those in the pupae not exposed to the mite. TRIzol reagent (Invitrogen) extraction of total RNA from individual whole pupae. Purification of the 15 to 40 nt RNA fraction using gel extraction. Small RNA (siRNA and miRNA) library cloning using Illumina TruSeq Small RNA Sample Preparation Kit according to manufactirers instructions
characteristics.characteristic: description
characteristics.text: pupa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0003143
characteristics.characteristic: developmental stage
characteristics.text: YL4
characteristics.characteristic: individual
characteristics.text: Apis mellifera
characteristics.characteristic: organism
characteristics.text: Apis mellifera
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7460
characteristics.characteristic: organism
characteristics.text: Apis mellifera
characteristics.characteristic: scientific_name
characteristics.text: Group VL - Pool YL4
characteristics.characteristic: title