characteristics.text: 2012-12-31T17:01:41Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:47:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SRA-Webin
characteristics.characteristic: ENA-SUBMISSION-TOOL
characteristics.text: SAMEA2008554
characteristics.characteristic: External Id
characteristics.text: BCCS
characteristics.characteristic: INSDC center name
characteristics.text: 2012-12-31T17:01:41Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:47:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS148512
characteristics.characteristic: SRA accession
characteristics.text: HI045_s_4_PT9
characteristics.characteristic: Submitter Id
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: characteristics = PT9 - lymphoblastoid cell line generated from a severely affected CdLs patient (Liu, J., Zhang, Z., Bando, M., Itoh, T., Deardorff, M.A., Clark, D., Kaur, M., Tandy, S., Kondoh, T., Rappaport, E., Spinner, N.B., Vega, H., Jackson, L.G., Shirahige, K., and Krantz, I.D. 2009. Transcriptional dysregulation in NIPBL and cohesin mutant human cells. PLoS Biol 7(5): e1000119.) characteristics = Chromatin immunoprecipitation for NIPBL growth_protocol = Lymphoblastoid cell lines derived from controls and Cornelia de Lange syndrome patients (Figure 5B) were obtained from Ian Krantz (The Children�s Hospital of Philadelphia, Philadelphia, Pennsylvania, United States of America) and cultured in RPMI medium supplemented with 0.2mM L-glutamine, 100 units per ml penicillin, 100 mg per ml streptomycin, 20% FCS. description = Polyclonal rabbit anti-NIPBL antibody raised against residues 2598-2825 of the X. laevis Scc2-1B and purified using the epitope used for immunization (133M, gift from Koichi Tanaka and Kim Nasmyth) was used for ChIP as described in (Wendt, K.S., Yoshida, K., Itoh, T., Bando, M., Koch, B., Schirghuber, E., Tsutsumi, S., Nagae, G., Ishihara, K., Mishiro, T., Yahata, K., Imamoto, F., Aburatani, H., Nakao, M., Imamoto, N., Maeshima, K., Shirahige, K., and Peters, J.M. 2008. Cohesin mediates transcriptional insulation by CCCTC-binding factor. Nature 451(7180): 796-801.).
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: CDL patient 2
characteristics.characteristic: title