characteristics.text: 2013-07-19T17:01:13Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:30:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2189649
characteristics.characteristic: External Id
characteristics.text: Physiological Genomics and Medicine Group, Imperial College London
characteristics.characteristic: INSDC center name
characteristics.text: 2013-07-19T17:01:13Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:30:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS252154
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1702:BN4
characteristics.characteristic: Submitter Id
characteristics.text: 6 week
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000034
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Norway rat
characteristics.characteristic: common name
characteristics.text: Protocols: Rats were housed in an air-conditioned animal facility and allowed free access to standard laboratory chow and water. All experiments were done in agreement with the Animal Protection Law of the Czech Republic and were approved by the Ethics Committee of the Institute of Physiology, Czech Academy of Sciences, Prague. We collected left ventricular tissue from unfasted males from six week old male BN-Lx/Cub (referred to in this study as BN), SHR/Olalpcv (referred to as SHR) (n = 4 per strain); and from (BNxSHR)F1 and (SHRxBN)F1 (n = 4 per cross); and from male rats from 29 BXH/HXB recombinant inbred strains (n = 2 per strain) (Pravenec et al. 1989) between 9:00am and 10:00am. Left ventricles were snap frozen in liquid nitrogen and stored at -80°C. The BN-Lx/Cub and SHR/Olalpcv lines have been inbred over more than 80 generations (Simonis et al. 2012). RNA was extracted from 25mg of crushed left ventricular tissue from four BN and four SHR animals without pooling, using Trizol (Invitrogen) according to manufacturer's instructions. 4ug of total RNA was used to generate RNA-seq libraries using TruSeq RNA kit (Illumina) according to manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: Rattus norvegicus
characteristics.characteristic: organism
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: left ventricle
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002084
characteristics.characteristic: organism part
characteristics.text: Rattus norvegicus
characteristics.characteristic: scientific_name
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: BN-Lx/Cub
characteristics.characteristic: strain
characteristics.text: BN4
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: title