characteristics.text: 2012-12-31T17:01:41Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:47:02Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SRA-Webin
characteristics.characteristic: ENA-SUBMISSION-TOOL
characteristics.text: SAMEA2008519
characteristics.characteristic: External Id
characteristics.text: BCCS
characteristics.characteristic: INSDC center name
characteristics.text: 2012-12-31T17:01:41Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:47:02Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS148505
characteristics.characteristic: SRA accession
characteristics.text: Smc1
characteristics.characteristic: Submitter Id
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: characteristics = 1-7HB2 cells(Bartek et al. 1991) characteristics = Chromatin immunoprecipitation for SMC1 growth_protocol = HB2 cells (1-7HB2, a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7, (Bartek et al. 1991)) were cultured in DMEM supplemented with 0.2 mM L-glutamine, 100 units/ml penicillin, 100 mg/ml streptomycin, 10% FCS, 5 �g/ml hydroxycortisone and 10 �g/ml human insulin SMC1_description = polyclonal rabbit anti-SMC3 antibodies (Bethyl Laboratories A300-055A) was used for ChIP under SDS-free conditions as described in (Schmidt, D., Wilson, M.D., Spyrou, C., Brown, G.D., Hadfield, J., and Odom, D.T. 2009. ChIP-seq: using high-throughput sequencing to discover protein-DNA interactions. Methods 48(3): 240-248). SMC1_input_description = input control sample prepared under SDS-free conditions as described in (Schmidt, D., Wilson, M.D., Spyrou, C., Brown, G.D., Hadfield, J., and Odom, D.T. 2009. ChIP-seq: using high-throughput sequencing to discover protein-DNA interactions. Methods 48(3): 240-248).
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Smc1 Chip Seq sample
characteristics.characteristic: title