characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-03-17T17:02:51Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T19:39:38Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2697752
characteristics.characteristic: External Id
characteristics.text: Genetwister Technologies B.V.
characteristics.characteristic: INSDC center name
characteristics.text: 2015-03-17T17:02:51Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T19:39:38Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS525800
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2825:Sample 5
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: trumpet lily
characteristics.characteristic: common name
characteristics.text: Vernalized for 5 weeks at 4C Protocols: L. longiflorum bulbs (cultivar White Heaven) were obtained from a nursery at the end of the growing season (August). Bulbs were sanitized and stored in humid standard pot medium at 25C (control, 0W) or at 4C for 2, 5, 7 or 9 weeks (2W, 5W, 7W, 9W, respectively). At each of these time points, shoot apical meristems were excised from the bulbs, immediately frozen in liquid nitrogen and then stored at -80C until RNA extraction. RNA was isolated using the AurumTM Total RNA mini kit (Biorad) following manufacturers instructions. The procedure was followed by an additional DNAse treatment with TURBO DNase (Life technologies) to remove all genomic DNA still present after the isolation. The RNA integrity was confirmed using the 2100 Bioanalyzer (Agilent Technologies). The samples were prepared for transcriptome sequencing using the Illumina kit (TruSeq RNA Sample Preparation Kit v2) following the manufacturers recommendations.
characteristics.characteristic: description
characteristics.text: Lilium longiflorum
characteristics.characteristic: organism
characteristics.text: Lilium longiflorum
characteristics.characteristic: organism
characteristics.text: apical shoot meristem
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0020148
characteristics.characteristic: organism part
characteristics.text: Lilium longiflorum
characteristics.characteristic: scientific_name
characteristics.text: Sample 5
characteristics.characteristic: title