characteristics.text: 2012-03-13T17:00:57Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:39:13Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1095981
characteristics.characteristic: External Id
characteristics.text: EMBL
characteristics.characteristic: INSDC center name
characteristics.text: 2012-03-13T17:00:57Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:39:13Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS104126
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-888:Mbd3_null1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: embryonic stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002322
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: ES cells were grown in GMEM supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 2 mM L-glutamine, 0.1 mM non-essential amino acids and 10 ng/ml murine LIF. Eed-/- ES cells were cultured on mitotically inactivated primary mouse embryonic fibroblasts which were depleted from ES cells upon harvest. TSA (Sigma) was added to normal ES cell media to a final concentration of 0.1 mM for 2 h prior to harvesting. Cells were fixed using 1% formaldehyde for 10min at room temperature and fixation quenched with 150 mM glycine. For Mi2beta ChIP-seq, formaldehyde fixation was preceded by 45 min incubation in 2 mM disuccinimidyl glutarate (Sigma). Chromatin was sheared by sonication to an average fragment size between 200 and 300 bp using a Bioruptor sonication instrument (Diagenode).
characteristics.characteristic: description
characteristics.text: Mdb3 -/-
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Mbd3_null1
characteristics.characteristic: title