characteristics.text: 2012-11-26T17:02:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:08:42Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1573961
characteristics.characteristic: External Id
characteristics.text: University of Helsinki, Genome-Scale Biology Program
characteristics.characteristic: INSDC center name
characteristics.text: 2012-11-26T17:02:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:08:42Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS195284
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1364:Pros45_8-1
characteristics.characteristic: Submitter Id
characteristics.text: 8
characteristics.characteristic: batch
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: S2
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001233
characteristics.characteristic: cell line
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: Drosophila S2 cells were cultured at controlled temperature (+23.5C; Binder KB cooling incubator) in Invitrogen SFM medium supplemented with 10% fetal bovine serum, 10% IMS (insect medium supplement, Sigma), 2 mM glutamine, and antibiotics. For transfections in 6-well plates, 1.000.000 S2 cells were seeded per well in 2 ml medium. The following day, cells were transfected with dsRNA according to manufacturers (Qiagen) instructions, using 5ul Effectene, 8.8 ul Enhancer, 1100 ng dsRNA. RNA extraction, was performed with Qiagen RNeasy kits according to manufacturers instruction, including the additional DNase treatment.
characteristics.characteristic: description
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: Pros45_8-1
characteristics.characteristic: title