characteristics.text: 2014-01-29T17:00:49Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:21:52Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2062263
characteristics.characteristic: External Id
characteristics.text: Institut de la Recherche Agronomique
characteristics.characteristic: INSDC center name
characteristics.text: 2014-01-29T17:00:49Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:21:52Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS234333
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1587:PDG2
characteristics.characteristic: Submitter Id
characteristics.text: 2
characteristics.characteristic: biological replicate
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: granulosa cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000501
characteristics.characteristic: cell type
characteristics.text: sheep
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/LBO_0001041
characteristics.characteristic: common name
characteristics.text: Protocols: Laser Capture Microdissection from Transcriptome profiling of sheep granulosa cells and oocytes during early follicular development obtained by Laser Capture Microdissection Bonnet et al 2010 (doi: 10.1186/1471-2164-12-417) RNA extraction from Transcriptome profiling of sheep granulosa cells and oocytes during early follicular development obtained by Laser Capture Microdissection Bonnet et al 2010 (doi: 10.1186/1471-2164-12-417) T7 linear amplification from Transcriptome profiling of sheep granulosa cells and oocytes during early follicular development obtained by Laser Capture Microdissection Bonnet et al 2010 (doi: 10.1186/1471-2164-12-417) All RNAseq libraries were constructed from 200 ng of aRNA using Illumina TruSeq RNA Sample Prep (Illumina, San Diego, CA USA) following manufacturers instruction. Each biological replicate was identified by a different index. Libraries were quantified using QPCR NGS Library Quant Kit (Agilent). Three libraries were pooled and loaded randomly per lane at 8 pM concentration.
characteristics.characteristic: description
characteristics.text: primordial follicle
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0003981
characteristics.characteristic: developmental stage
characteristics.text: Ovis aries
characteristics.characteristic: organism
characteristics.text: Ovis aries
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9940
characteristics.characteristic: organism
characteristics.text: ovary
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000992
characteristics.characteristic: organism part
characteristics.text: Ovis aries
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: PDG2
characteristics.characteristic: title