characteristics.text: No
characteristics.characteristic: ClinicalInformation: Premortem diagnosis of diabetes
characteristics.text: 11.3 millimolar
characteristics.characteristic: ClinicalInformation: Premortem non-fasting glycemia
characteristics.text: 2012-10-03T17:04:06Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:57:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1531965
characteristics.characteristic: External Id
characteristics.text: Hospital Clinic de Barcelona, IDIBAPS, CIBERDEM
characteristics.characteristic: INSDC center name
characteristics.text: 2012-10-03T17:04:06Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:57:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS177650
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1294:HI 25
characteristics.characteristic: Submitter Id
characteristics.text: 59 year
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000036
characteristics.characteristic: age
characteristics.text: 24.2kg_per_m2
characteristics.characteristic: bmi
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: cause of death: ischemic brain injury
characteristics.characteristic: clinical history
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: All experiments were performed according to protocols approved by the Institutional ethical committees of the Hospital Clinic de Barcelona, Geneva University Hospitals, Istituto Scientifico Ospedale San Raffaele, Oxford University, UK Human Tissue Authority and University of Lille. Samples were isolated from multiorgan donors without a history of glucose intolerance after informed consent from family members. Donor variables and cold ischemia times of the samples used in this study are provided separately. Pancreatic islets were isolated and purified according to established isolation procedures. After isolation, islets were incubated at 37 degrees C in a humidified chamber with 5% CO2 in CMRL 1066 medium with 10% fetal calf serum for various periods prior to shipment at room temperature in the same culture medium. Upon arrival, islets were re-cultured at 37 degrees C in a humidified chamber with 5% CO2 in RPMI 1640 medium supplemented with 10% fetal calf serum, 100 U/ml penicillin, and 100 U/ml streptomycin for three days before extraction of RNA or chromatin. Islet purity was assessed by dithizone staining using an aliquot of islets immediately prior to harvest. Further assessment of islet purity was carried out by qPCR analysis of duct, acinar, and beta-cell specific markers (SOX9, CPA1, MIST1, NKX6.1, and INS). Only samples showing marginal exocrine contaminant mRNAs were further processed. Acinar-enriched fractions were obtained after gradient purification of islets.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: HI 25
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006980
characteristics.characteristic: title