characteristics.text: 71
characteristics.characteristic: %_templated_ISPs
characteristics.text: 15.8
characteristics.characteristic: Background_RFU_(negative_control)
characteristics.text: 171.95
characteristics.characteristic: Bioanalyzer,_only_fragments_of_interest
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-07-01T17:01:21Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:17:26Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2421485
characteristics.characteristic: External Id
characteristics.text: UWEST
characteristics.characteristic: INSDC center name
characteristics.text: 2015-07-01T17:01:21Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:17:26Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: Platinum_PCR_SuperMix_High_Fidelity_(Invitrogen)
characteristics.characteristic: PCR_amplification
characteristics.text: ERS426688
characteristics.characteristic: SRA accession
characteristics.text: Brain_Rno2
characteristics.characteristic: Submitter Id
characteristics.text: CCTTAGAGTTC
characteristics.characteristic: barcode sequence
characteristics.text: 63
characteristics.characteristic: barcode_no
characteristics.text: Huntingdon_Life_Sciences_Cambridge
characteristics.characteristic: collection site
characteristics.text: Norway rat
characteristics.characteristic: common name
characteristics.text: Sequencing_of_bacterial_16S_rRNA_gene_from_rat_brain.
characteristics.characteristic: description
characteristics.text: 88.2
characteristics.characteristic: enriched_ISPs
characteristics.text: 85.1
characteristics.characteristic: extraction
characteristics.text: PureLink_Genomic_DNA_Mini_Kit_(Invitrogen)_(modified_protocol)
characteristics.characteristic: nucleic acid extraction
characteristics.text: Rattus norvegicus
characteristics.characteristic: organism
characteristics.text: activation_(at_94_degrees_for_5_minutes)._40_cycles_of:_denature_(at_94_degrees_for_30_seconds),_anneal_(at_55_degrees_for_30_seconds),_extend_(at_64_degrees_for_1_minute)._final_extension_(at_68_degrees_for_10_minutes)
characteristics.characteristic: pcr conditions
characteristics.text: forward_primer:_S-D-Bact-0341-b-S-17,_5'-CCTACGGGNGGCWGCAG-3',_reverse_primer:_S-D-Bact-0515-a-A-19,_5'-TTACCGCGGCTGCTGGCAC-3'
characteristics.characteristic: pcr primers
characteristics.text: R_2012_05_21_03_54_52_user_SN1-31-Animal_Brain_Bugs
characteristics.characteristic: run id
characteristics.text: Rattus norvegicus
characteristics.characteristic: scientific_name
characteristics.text: Ion_PGM
characteristics.characteristic: sequencing_methods
characteristics.text: brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: tissue site
characteristics.text: Rat olfactory bulb 16S V3 amplicon
characteristics.characteristic: title
characteristics.text: 1262.6
characteristics.characteristic: unenriched_ISPs