characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-12-17T13:48:31Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T22:39:40Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3177006
characteristics.characteristic: External Id
characteristics.text: University of Manchester
characteristics.characteristic: INSDC center name
characteristics.text: 2015-12-17T13:48:31Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T22:39:40Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS629938
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3179:P63a_rep1_614EGAAXX_4_1208
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Palatal shelves dissected from E13.5/E14.5 mice were cross-linked with 1% formaldehyde for 20 minutes. After reverse cross-link and protein digestion with Proteinase K (Roche), DNA was purified using Qiaquick, PCR purification kit (Qiagen) following the manufacturer instructions. Chromatin was prepared by homogenising the tissue pellet in PBS using an Ultra-Turrax Homogeniser, after which the palatal cells were incubated in PIPES buffer according to the Upstate ChIP assay protocol (Merck Millipore). Chromatin was sonicated using a Sonics Vibracell with seven 10 second pulses set to an amplitude of 40. Chromatin was initially prewashed with Protein A-Agarose beads (SC-2001; Santa Cruz,) and subsequently incubated with 2 μg of α isoform-specific p63 antibody (H129; Santa Cruz) overnight at 4 degree C. Antibody-bound chromatin was recovered using protein A-agarose beads (Santa Cruz).
characteristics.characteristic: description
characteristics.text: E13.5/E14.5
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: palatal shelves
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: P63a_rep1_614EGAAXX_4_1208
characteristics.characteristic: title