characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-05-15T17:02:21Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:21:50Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2464850
characteristics.characteristic: External Id
characteristics.text: University of Toronto
characteristics.characteristic: INSDC center name
characteristics.text: 2014-05-15T17:02:21Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:21:50Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS434656
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2497:cell_culture_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HEK293
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001182
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: The HEK293_M2 + RHOXF2 stable cell line (PB-TGcMV-Neo) were seeded in 10cm-dishes, cultivated 24 hours in the presence or absence of doxycycline (2microgram/ml final) before been treated with 40nM of mitomycin C or DMSO (control) during a 2-days period. Total RNA was extracted (RNeasy Mini Kit - Qiagen), mRNA-focused libraries was generated from 1ug of total RNA (Illumina TruSeq RNA Sample Preparation Kit V.2). Cells were treated with 40nM of mitomycin C or DMSO (control) during a 2-days period. Total RNA was extracted (RNeasy Mini Kit Qiagen) mRNA-focused libraries was generated from 1ug of total RNA (Illumina TruSeq RNA Sample Preparation Kit V.2)
characteristics.characteristic: description
characteristics.text: RHOXF2 overexpression in HEK293
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: cell_culture_1
characteristics.characteristic: title