characteristics.text: 2012-11-22T17:01:46Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:40:52Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1570641
characteristics.characteristic: External Id
characteristics.text: University of Cologne, Cologne Center for Genomics
characteristics.characteristic: INSDC center name
characteristics.text: 2012-11-22T17:01:46Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:40:52Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS123130
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1043:Ler x Col
characteristics.characteristic: Submitter Id
characteristics.text: 10 day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: A. thaliana plants of the accessions Col and Ler were used as genetic background. For intraspecific crossing, Col and Ler plants were grown on soil in LD conditions. Five flower buds on the primary shoot and two side shoots were emasculated and manually cross pollinated, while all other flower buds were removed. The seeds of each genotype were pooled and the success rate of the crosses was determined by PCR using primers that detected an insertion between Ler and Col (fw: 5-ctggagatcatccaacaaagg-3, rv: 5-ggcaatggaatgggctggtc-3). Seed pools with less than 10 % maternal contamination were used in the F1 hybrid studies. Hybrids of the F1 generation were grown in LD (16-hours light, 8-hours dark) at 20C constant temperature on Murashige and Skoog (GM) medium supplemented with 1 % sucrose after stratification at 4C for 2-4 days to synchronize germination. Light was provided by fluorescent tubes complemented by incandescent bulbs to increase the proportion of far red light. Nuclear extraction and ChIP experiments were performed as described in J.J. Reimer and F. Turck; Genome-wide mapping of protein-DNA interaction by chromatin immunoprecipitation and DNA microarray hybridization (ChIP-chip). Part A: ChIP-chip molecular methods, Methods Mol Biol, 631, 139-160, 2010.
characteristics.characteristic: description
characteristics.text: seedling
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007131
characteristics.characteristic: developmental stage
characteristics.text: Ler x Col
characteristics.characteristic: genotype
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: Ler x Col
characteristics.characteristic: title