characteristics.text: 2011-10-24T15:04:21Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:29:33Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1094184
characteristics.characteristic: External Id
characteristics.text: CRI
characteristics.characteristic: INSDC center name
characteristics.text: 2011-10-24T15:04:21Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:29:33Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS054478
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-785:MCF-7
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: MCF-7
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001203
characteristics.characteristic: cell line
characteristics.text: epithelial
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000066
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: MCF7 cells were grown in DMEM medium, supplemented with 8% FBS and standard antibiotics. Three days prior to hormonal treatment, cells were switched to phenolred-free DMEM, supplemented with 5% charcoal-treated FBS and standard antibiotics. Medium was replaced daily. Serum-deprived MCF7 cells were treated with 10-7 M Estradiol in 20ml medium for 2 hours and 50 minutes, after which cells were fixed with1% PFA and processed as described (Schmidt et al., Methods 48:3 pp240-2482009). Cell lysis and precipitation procedures were performed as described (Schmidt et al., Methods 48:3 pp240-248 2009).
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: breast
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000310
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: MCF-7
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001203
characteristics.characteristic: title