characteristics.text: 2013-03-08T17:02:32Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:18:54Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1713777
characteristics.characteristic: External Id
characteristics.text: Institute for Systems Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2013-03-08T17:02:32Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:18:54Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS221127
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1538:B6_PBS_8h
characteristics.characteristic: Submitter Id
characteristics.text: 8 week
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000034
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: The mice were euthanized at different time points post exposure (at 3, 8, 24, 48 and 72 hours); Eight week old female C57 Black mice were fasted for 24 hours prior to a single intraperitoneal injection of 350mg/kg of acetaminophen in phosphate buffer saline (PBS) (treatment group) or PBS (control group) Liver and plasma samples were collected from different time points after mice had been exposed to acetaminophen. The plasma was prepared from EDTA blood by centrifugation at 1000 x g for 15 minutes to separate the plasma and blood cells. Total RNA from liver and plasma was extracted with the miRNeasy kit (Qiagen, Germantown, MD), as described. The quality and quantity of the RNA were evaluated with Agilent 2100 Bioanalyzer (Santa Clara, CA) and NanoDrop 1000 spectrophotometer (Thermo Scientific, Wilmington, DE). we constructed a single library with an insert size ranging from 20 to 100 nucleotides long. We also did not conduct mRNA purification and fragmentation steps prior to library construction since we did not observe ribosomal RNA peaks from plasma RNA on the bioanalyzer, and most of the transcripts in circulation were probably not full-length based on prior studies. This approach allowed us to obtain a general profile for both miRNA and mRNA in the plasma from a single library.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: plasma
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001969
characteristics.characteristic: organism part
characteristics.text: control
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001461
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: B6_PBS_8h
characteristics.characteristic: title