characteristics.text: 2013-05-21T11:50:03Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:27:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1906773
characteristics.characteristic: External Id
characteristics.text: Wellcome Trust Sanger Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2013-05-21T11:50:03Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:27:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS246978
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1665:polyA+_lung_1100350
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Human polyA+ RNAs were purchased from Clontech. The RNAs were isolated from tissue samples using a modified guanidium thiocynate extraction method followed by polyA+ RNA selection with two rounds of oligo(dT)-cellulose columns. 5' and 3' RACE reactions were performed in 12.5 ul volume independently with each polyA+ RNA sample using the SMART RACE amplification kit (Clontech) according to the manufacturer’s instructions. 7.5ul of each of the 400 lncRNA targets were pooled respecting tissues and directionality of the RACE reaction (5' and 3' RACE) and purified with the QIAquick kit (Quiagene) as described for RT-PCR-seq experiments [2]. A set of 25 control reactions targeting full-length annotated transcripts were RACEd in parallel as positive controls. 1.5ul of these controls were pooled with the other reactions prior to library preparation. Libraries were prepared according to the Rapid Library Preparation Method Manual for GS FLX+ Series / XL+ Kit.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: lung
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002048
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: mixed sex
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: frozen sample
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: polyA+_lung_1100350
characteristics.characteristic: title