characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-09-21T17:01:06Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T20:19:36Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2782815
characteristics.characteristic: External Id
characteristics.text: Swedish University of Agricultural Sciences, SLU
characteristics.characteristic: INSDC center name
characteristics.text: 2015-09-21T17:01:06Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T20:19:36Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS552560
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2953:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: black nightshade
characteristics.characteristic: common name
characteristics.text: Leaves were collected before infection and at 24 and 48 hours after infection by P. infestans (isolate SE-03058). Samples were pooled for each species. Protocols: RNA was isolated by RNeasy Plant Mini Kit (Qiagen) Beads with oligo(dT) were used to isolate poly(A) mRNA. Fragmentation buffer was added for interrupting mRNA to short fragments. Taking these short fragments as templates, Random hexamerprimer were used to synthesize the first-strand cDNA. The second-strand cDNA was synthesized using buffer, dNTPs, RNase H and DNA polymerase I, respectively. Short fragments were purified with QiaQuick PCR extraction kit and resolved with EB buffer for end reparation and adding poly(A). After that, the short fragments were connected with sequencing adaptors. And, for amplification with PCR, we selected suitable fragments, as templates, with respect to the result of agarose gel electrophoresis.
characteristics.characteristic: description
characteristics.text: Solanum nigrum
characteristics.characteristic: organism
characteristics.text: Solanum nigrum
characteristics.characteristic: organism
characteristics.text: detached leaves
characteristics.characteristic: organism part
characteristics.text: Solanum nigrum
characteristics.characteristic: scientific_name
characteristics.text: Sample 1
characteristics.characteristic: title