characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-09-30T17:24:33Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T20:14:32Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2747218
characteristics.characteristic: External Id
characteristics.text: The University of Manchester
characteristics.characteristic: INSDC center name
characteristics.text: 2015-09-30T17:24:33Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T20:14:32Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS540022
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2892:LC8
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: baker's yeast
characteristics.characteristic: common name
characteristics.text: Protocols: Grown in SCD media lacking uracil at 30C to exponential phase (OD600 ~0.6), pelleted by centrifugation and snap frozen into liquid nitrogen. Samples lysed in Buffer A using a 6870 Freezer Mill (Spex). Lysates cleared by centrifuging twice at 15000 g. Nucleic acids extracted using Trizol Reagent. rRNA was depleted from samples using Ribo-ZeroTM Magnetic Gold Kit for RNA-Seq. Depleted samples were precipitated with ethanol, and re-suspended in 10ul DEPC water. Sequencing libraries were then generated using the TruSeq Stranded mRNA sample preparation guide from ilumina from the Incubate RFP step, with 5ul of the ribosome depleted RNA added to 13ul of the fragment prime and finish mix.
characteristics.characteristic: description
characteristics.text: targeted mutation
characteristics.characteristic: genetic modification
characteristics.text: his3delta1; leu2delta0; met15delta0; ura3delta0; caf20::G418; pCAF20m2-FLAG::URA3
characteristics.characteristic: genotype
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4932
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: scientific_name
characteristics.text: Mat a
characteristics.characteristic: sex
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: BY4741
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000098
characteristics.characteristic: strain
characteristics.text: LC8
characteristics.characteristic: title