characteristics.text: 2012-11-20T17:02:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:08:55Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1573841
characteristics.characteristic: External Id
characteristics.text: Cancer Research UK
characteristics.characteristic: INSDC center name
characteristics.text: 2012-11-20T17:02:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:08:55Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS195084
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1368:Jc32_2
characteristics.characteristic: Submitter Id
characteristics.text: frozen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: bio source type
characteristics.text: ATCC
characteristics.characteristic: biosourceprovider
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: MCF-7
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001203
characteristics.characteristic: cell line
characteristics.text: breast epithelial
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000066
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: MCF-7 cells were grown in DMEM containing 10% heat-inactivated FBS, 2 mM L glutamine, 50 U/ml penicillin and 50 _g/ml streptomycin at 37oC with 5% CO2. To hormone deprive the cells, cells were grown in steroid-depleted (phenol red free DMEM containing 5% heat-inactivated CDT FBS, 2 mM L glutamine, 50 U/ml penicillin and 50 _g/ml streptomycin) medium for three days and then treated with vehicle (ethanol), 100 nM 17b-Estradiol (Sigma E2758) (ER ChIP) or 60 mins (PolII ChIP). siRNA experiments were performed using Lipofectamine2000 (Invitrogen, CA, USA) 25uL was used in a total of 5mls of Optimem (Invitrogen, CA, USA) together with 50nM of siRNA for 6 hours. The sequence of the siRNA was: siTLE1 (D-015528-17): GGAAAAUGGAAUCGACAAA. After 48h of transfection, cells were treated with control ethanol vehicle or 17b-Estradiol.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Jc32_2
characteristics.characteristic: title