characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-02-16T17:01:59Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T18:23:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2579683
characteristics.characteristic: External Id
characteristics.text: UCSF
characteristics.characteristic: INSDC center name
characteristics.text: 2015-02-16T17:01:59Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T18:23:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS472805
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2624:Beige_biolrepli2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: rosiglitazone-treated adipocyte (beige cell)
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Primary stromal vascular (SV) cells were isolated from inguinal WAT or from interscapular BAT of the same C57BL/6 mice using Collagenase D (1.5 u/ml) and Dispase II (2.4 u/ml). To clearly define the molecular signatures of PPAR-gamma ligand-induced beige cells, we used C57BL/6 mice that possess less beige cells in the absence of environmental stimuli. SV cells were plated in collagen coated culture dishes in DMEM/F12 medium (D-glucose 17.51 mM) and differentiated as described previously (DOI: 10.1371/journal.pone.0049452). Beige cells were induced by a specific PPAR-gamma agonist rosiglitazone (1uM). RiboZol reagent (AMRESCO) following the manufacturer's protocol Ultralow DR library kit (NuGEN): www.nugeninc.com/nugen/index.cfm/products/cs/ngs/rna-seq-v2
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Beige_biolrepli2
characteristics.characteristic: title