characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-04-24T17:23:28Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T21:49:25Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3138157
characteristics.characteristic: External Id
characteristics.text: Rothamsted Research
characteristics.characteristic: INSDC center name
characteristics.text: 2015-04-24T17:23:28Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T21:49:25Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS610092
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3103:IP1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: bread wheat
characteristics.characteristic: common name
characteristics.text: Holdfast
characteristics.characteristic: cultivar
characteristics.text: Protocols: Immature grain at 12 days post-anthesis were collected from floret positions 1 & 2 of spikelets in the central 3/4 of the ears. Three tissue layers were isolated by manual dissection: the 'outer pericarp', which included the cuticle, outer epidermis, hypodermis and parenchyma cells; the 'inner pericarp' which comprised the cross cells, inner epidermis, integuments and aleurone; and the endosperm. Tissues were frozen in liquid nitrogen and stored at -80oC Plants growth in 15cm pots of a peat and loam-based compost in a glasshouse: 16 hr day (), 8 hr night (). Plant were tagged at anthesis. RNA was extracted according to Wan et al. (2009) Plant Biotechnol J 7, 401-410. Libraries were prepared using the Illumina TruSeq mRNA sample kit using 4ug of total RNA as described in the Illumina TruSeq RNA sample preparation guide, Part 15008136 Rev A, Nov 2010.
characteristics.characteristic: description
characteristics.text: 12 days post anthesis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007016
characteristics.characteristic: developmental stage
characteristics.text: Triticum aestivum
characteristics.characteristic: organism
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: inner pericarp
characteristics.characteristic: organism part
characteristics.text: Triticum aestivum
characteristics.characteristic: scientific_name
characteristics.text: IP1
characteristics.characteristic: title