characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-04-15T17:01:39Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:47:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2201329
characteristics.characteristic: External Id
characteristics.text: EMBL-EBI
characteristics.characteristic: INSDC center name
characteristics.text: 2016-04-15T17:01:39Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:47:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS353300
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1913:ir29_salt_1hr_rep3
characteristics.characteristic: Submitter Id
characteristics.text: 2 week
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000034
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: long-grained rice
characteristics.characteristic: common name
characteristics.text: IR29
characteristics.characteristic: cultivar
characteristics.text: Protocols: Oryza sativa L. Indica IR29 seedlings were grown in growth chambers to the 2-3 leaf stage. At 2 weeks old, they were subjected to salt stress treatment by watering with 300 mM NaCl solution (or normal watering for control plants). Treatment was applied at 10:00 PST (2 hours post-dawn). Seedling shoots (stem and leaves) were sampled at 1 hour, 2 hours, 5 hours, 10 hours, and 24 hours post-treatment. Total cellular RNA was extracted using RNA Plant Reagent (Life Technologies) and treated with RNase-free Turbo DNase (Life Technologies) followed by a clean-up step using Plant RNeasy kits (Qiagen). Concentration, integrity and extent of contamination by ribosomal RNA were assessed using ND-1000 spectrophotometer (Thermo Fisher Scientific) and Bioanalyzer 2100 (Agilent Technologies). For details see Fox et al. (2009) Applications for Ultra-high-Throughput Sequencing. In: Belostotsky (ed.), Plant Systems Biology, vol. 553, pp. 79--108. Libraries were prepared using the TruSeq(TM) RNA Sample Preparation Kits (v2).
characteristics.characteristic: description
characteristics.text: seedling, two leaves visible, three leaves visible
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007098
characteristics.characteristic: developmental stage
characteristics.text: Oryza sativa Indica group
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_39946
characteristics.characteristic: organism
characteristics.text: Oryza sativa Indica Group
characteristics.characteristic: organism
characteristics.text: shoot, vascular leaf
characteristics.characteristic: organism part
characteristics.text: Oryza sativa Indica Group
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: ir29_salt_1hr_rep3
characteristics.characteristic: title