characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-08-01T17:01:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:21:14Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2465341
characteristics.characteristic: External Id
characteristics.text: The University of Manchester
characteristics.characteristic: INSDC center name
characteristics.text: 2014-08-01T17:01:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:21:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS435106
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2464:eIF4E-IP(2)
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: baker's yeast
characteristics.characteristic: common name
characteristics.text: Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit
characteristics.characteristic: description
characteristics.text: eIF4E-tap-tag
characteristics.characteristic: genotype
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: organism
characteristics.text: Saccharomyces Cerevisiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4932
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: scientific_name
characteristics.text: Mat a
characteristics.characteristic: sex
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: BY4741
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000098
characteristics.characteristic: strain
characteristics.text: eIF4E-IP(2)
characteristics.characteristic: title