characteristics.text: 2015-02-02T17:02:16Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:23:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1970328
characteristics.characteristic: External Id
characteristics.text: University of Manchester
characteristics.characteristic: INSDC center name
characteristics.text: 2015-02-02T17:02:16Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:23:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS240638
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1631:Meis IBA input exp2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: The preparation of the material was as described in Kutejova et al., 2008. First branchial arches are dissected (the whole tissue), crosslinked and frozen in order to pool enough for ChIP. Kutejova E, Engist B, Self M, Oliver G, Kirilenko P, Bobola N. Six2 functions redundantly immediately downstream of Hoxa2. Development. 2008. 135(8):1463-70. PMID: 18321982. ChIP assays were performed as described in Kutejova et al., 2008, with the following modifications for ChIP-seq. The crosslinked material was sonicated to 300 bp fragments (Vibracell sonicator, Sonics: 8 times for 10 seconds at 50% output). Immunoprecipitation was performed starting from 12 pairs of first branchial arches from E11.5 embryos and incubating overnight at 4oC with 3 ug of pan-Meis antibodies or control IgG (Kutejova et al., 2008), followed by protein A beads (Invitrogen). Kutejova E, Engist B, Self M, Oliver G, Kirilenko P, Bobola N. Six2 functions redundantly immediately downstream of Hoxa2. Development. 2008. 135(8):1463-70. PMID: 18321982.
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: 11.5 day
characteristics.characteristic: gestational age
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: first branchial arch
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: mixed sex
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: Meis IBA input exp2
characteristics.characteristic: title