characteristics.text: 2014-03-22T17:00:38Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:09:24Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2040037
characteristics.characteristic: External Id
characteristics.text: 1 Universite de Toulouse ; UPS ; LBCMCP ; 118 route de Narbonne, 31062, Toulouse, France
characteristics.characteristic: INSDC center name
characteristics.text: 2014-03-22T17:00:38Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:09:24Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS195674
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1241:XRCC4_2 ChIP
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: AsiSI-ER-U20S
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: 4-hydroxytamoxifen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_44616
characteristics.characteristic: compound
characteristics.text: Protocols: U20S AsiSI-ER cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with antibiotics and 10% FCS (Invitrogen) at 37 C under a humidified atmosphere with 5% CO2. Cells were treated with 300 nM 4OHT for 4 hours ChIP assays were carried out according the protocol described in (Iacovoni et al, 2010). Briefly, 200 ug of chromatin was subjected to immunoprecipitation using 2 ug of a gammaH2AX antibody (Epitomics 2212-1), XRCC4 (Abcam), or Rad51 (Santa Cruz). For ChIP against PolII-S2P (Abcam) 50 ug of chromatin was used, instead of 200 ug. After the washes the beads/chromatin complexes were re-suspended in 200 uL of TE buffer (Tris 10mM pH8, EDTA 0,5mM pH8) and were directly subjected to crosslink reversal in presence of 0,5% SDS overnight at 70 C. Following crosslink reversion, all immunoprecipitated DNA and input DNA were purified by phenol/chloroform, followed by DNA precipitation. DNA amount was quantified using the Quant-iTTM kit from Invitrogen. 10ng of DNA was next used for sequencing.
characteristics.characteristic: description
characteristics.text: 300 nanomolar
characteristics.characteristic: dose
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: XRCC4_2 ChIP
characteristics.characteristic: title