characteristics.text: 2013-06-03T13:10:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:25:52Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1906697
characteristics.characteristic: External Id
characteristics.text: University of Glasgow
characteristics.characteristic: INSDC center name
characteristics.text: 2013-06-03T13:10:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:25:52Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS244693
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1668:24hCR-RNA
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: Arabidopsis thaliana (Col-0) seeds were germinated on vertical agar plates containing 70 ml of nutrient solution containing 0.5 mM CaCl2, 0.25 mM MgSO4, 2 mM KNO3, 1 mM NaCl, 0.5 mM NaH2PO4, 42.5 uM Fe(III)Na-EDTA, 1.8 uM MnSO4, 45 uM H3BO3, 0.38 uM ZnSO4, 0.015 uM (NH4)6Mo7O24, 0.16 uM CuSO4, and 0.01 uM CoCl2, supplemented with 3% sucrose and 1% agar. Plants were grown at 22 degreesC with a 10/14 h light/dark period and a light intensity of 120 umol m-2s-1. When seedlings had reached the 4-leaf stage the priming treatment was applied directly to the roots. Roots and shoots were harvested immediately after the priming treatment . The tissue was stored at -80 degreesC. The priming treatment consisted in application of 5 ml of growth medium supplemented with 50 mM NaCl directly to the roots of the plate-grown seedlings. Control plants were treated in the same way using nutrient medium without NaCl added. Plants were incubated with the priming (or control) solution for 24 hours. After grinding of the frozen tissues, RNA was extracted with the RNeasy mini kit (Qiagen) according to the manufacture instructions, and reverse transcribed to cDNA using QuantiTect(r) Reverse Transcription kit (Qiagen). RNA-seq libraries were prepared using mRNA 8-Sample Prep Kit (Illumina). Briefly, polyA RNA was isolated from 1 ug of total RNA, fragmented, and subjected to first- and second-strand cDNA synthesis with random primers.
characteristics.characteristic: description
characteristics.text: Seedling 4L
characteristics.characteristic: developmental stage
characteristics.text: Columbia
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005147
characteristics.characteristic: ecotype
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: root
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: 24hCR-RNA
characteristics.characteristic: title