characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-10-02T17:03:58Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:47:27Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2220072
characteristics.characteristic: External Id
characteristics.text: University of Milan. Dept. of Biosciences
characteristics.characteristic: INSDC center name
characteristics.text: 2014-10-02T17:03:58Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:47:27Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS358323
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1946:ST3_R1
characteristics.characteristic: Submitter Id
characteristics.text: 3 day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: Plants of Arabidopsis thaliana ecotype Col-0 were grown under long day conditions (LD; 16-hours light/8-h dark). Tissue was sectioned into slices with a Leica RM 2155 microtome, distended on pure methanol (Schmid et al., 2012) on Leica PET membrane frameslides, dried at 45 degrees C for 45 minutes, washed for 15 minutes in pure xylol to remove Paraplast, dried again at room temperature for 10 minutes and immediately dissected with a Leica LMD6000 laser microdissector. Material from 3 slides was pooled in a single tube and immediately after microdissection 10 ul of XB buffer from Arcturus Picopure RNA extraction Kit (Life Technologies) was added. Samples were then heated at 42 degrees C for 30 minutes, centrifuged at 800 r.c.f for 2 minutes and stored at -80. Before proceeding with the extraction, material from 5 different tubes were pooled in one; therefore one single replicate contains material microdissected from 15 inflorescences, each harvested from a different plant. RNA extraction was then performed using the Arcturus Picopure RNA extraction kit as specified by the manufacturers instructions. RNA integrity and concentration was tested with an Agilent 2100 Bioanalyzer. Retro-transcription and amplification were performed using the Ovation RNAseq V2 kit (NuGen technologies) as specified by the manufacturers instructions.
characteristics.characteristic: description
characteristics.text: meristem stage 3
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007601
characteristics.characteristic: developmental stage
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: flower
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009046
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: ST3_R1
characteristics.characteristic: title