characteristics.text: 2014-01-11T17:01:04Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:09:56Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2041032
characteristics.characteristic: External Id
characteristics.text: University of Liverpool
characteristics.characteristic: INSDC center name
characteristics.text: 2014-01-11T17:01:04Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:09:56Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS197353
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1386:Young2_0h
characteristics.characteristic: Submitter Id
characteristics.text: 4 year
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000036
characteristics.characteristic: age
characteristics.text: abattoir
characteristics.characteristic: biosource provider
characteristics.text: fresh sample
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: biosource type
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: chondrocyte
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000138
characteristics.characteristic: cell type
characteristics.text: horse
characteristics.characteristic: common name
characteristics.text: Protocols: Full thickness cartilage was removed from the entire surface of MCPII.All samples were scored macroscopically to determine any pathological abnormalities of the distal condyles of the distal third metacarpus. For RNA extraction the Guanidium-thiocyanate-phenol-chloroform technique, with ethanol extraction was used (Chomczynski, P. and N. Sacchi, Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem, 1987
characteristics.characteristic: description
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease state
characteristics.text: Equus caballus
characteristics.characteristic: organism
characteristics.text: Equus caballus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9796
characteristics.characteristic: organism
characteristics.text: cartilage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0007844
characteristics.characteristic: organism part
characteristics.text: Equus caballus
characteristics.characteristic: scientific_name
characteristics.text: Young2_0h
characteristics.characteristic: title