characteristics.text: 2014-04-15T17:00:43Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:23:12Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1969582
characteristics.characteristic: External Id
characteristics.text: National Bureau of Plant Genetic Resources, New Delhi, India
characteristics.characteristic: INSDC center name
characteristics.text: 2014-04-15T17:00:43Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:23:12Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS239303
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1630:DS_drought
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: sorghum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4558
characteristics.characteristic: common name
characteristics.text: Protocols: Sorghum (Sorghum bicolor (L.) Moench) genotype drought tolerant and drought susceptible were grown at 28-32 degrees C day/night temperature with 12/12 h light/dark period in the phytotron glass house. Thirty days after sowing, drought stress was imposed by withholding the irrigation to one set of plants till the leaf relative water content reached to about 60-65%. Total RNA was isolated from the leaves using the TRIzol reagent (Invitrogen, USA), according to the manufacturer's protocol. The RNA quality was examined using gel electrophoresis (28S:18S > 1.5) and Bioanalyzer (Agilent 2100, RIN = 8.0). Small RNA sequencing libraries were prepared using Illumina Truseq small RNA Library preparation kit following manufacturer's protocol.
characteristics.characteristic: description
characteristics.text: 30 days old leaf
characteristics.characteristic: developmental stage
characteristics.text: drought stress
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/EO_0007404
characteristics.characteristic: environmental stress
characteristics.text: drought susceptible
characteristics.characteristic: genotype
characteristics.text: Sorghum bicolor
characteristics.characteristic: organism
characteristics.text: Sorghum bicolor
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4558
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organism part
characteristics.text: Sorghum bicolor
characteristics.characteristic: scientific_name
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: DS_drought
characteristics.characteristic: title