characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-07-14T16:44:21Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:16:41Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2418262
characteristics.characteristic: External Id
characteristics.text: Medical Research Council, National Institute for Medical Research
characteristics.characteristic: INSDC center name
characteristics.text: 2014-07-14T16:44:21Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:16:41Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS423783
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2393:110_5nM_FGF_12h
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: chicken
characteristics.characteristic: common name
characteristics.text: Protocols: Intermediate neural plate explants were isolated from HH stage 9 embryos and were cultured in collagen drops (SIGMA) with DMEM for indicated time periods. Chick intermediate neural explants were incubated in the F-12 medium (Gibco) for 0 hour (sample 106) or for 12 hours (samples 107 and 110) in the absence (sample 107) or in the presence of 5nM of bFGF (Fibroblast Growth Factor basic) (sample 110). picopure RNA extraction kit Library was constructed by TruSeq RNA sample preparation kit.
characteristics.characteristic: description
characteristics.text: HH stage 9 embryo
characteristics.characteristic: developmental stage
characteristics.text: Gallus gallus
characteristics.characteristic: organism
characteristics.text: Gallus gallus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9031
characteristics.characteristic: organism
characteristics.text: intermediate region of the neural plate
characteristics.characteristic: organism part
characteristics.text: Gallus gallus
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: 110_5nM_FGF_12h
characteristics.characteristic: title