characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-04-10T17:00:53Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:09:39Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2355623
characteristics.characteristic: External Id
characteristics.text: State University of Londrina
characteristics.characteristic: INSDC center name
characteristics.text: 2014-04-10T17:00:53Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:09:39Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS401600
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2301:Non-inoculated T. aestivum roots (sample 1)
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: bread wheat
characteristics.characteristic: common name
characteristics.text: Protocols: T. aestivum seeds were surface-sterilized and germinated (in water agar plates) at 30 degrees C for 12 h under darkness. Germinated seedlings were transferred to sterile glass tubes containing 25 mL Hoagland's nutrient solution (two seedlings per tube) and cultivated at 26 degrees C under 14 h light/10 h darkness for 24 h. In each tube were then added 0.25 mL of sterile Hoagland's nutrient solution and incubated for 3 days at 26 degrees C under 14 h light/10 h darkness. RNAqueous (Ambion) SOLiD Total RNA-Seq Kit (barcoded by Transcriptome Multiplexing Kit) (Life Technologies)
characteristics.characteristic: description
characteristics.text: 4 days old root
characteristics.characteristic: developmental stage
characteristics.text: not colonised by Azospirillum brasilense
characteristics.characteristic: growth condition
characteristics.text: Triticum aestivum
characteristics.characteristic: organism
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: root
characteristics.characteristic: organism part
characteristics.text: Triticum aestivum
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: Non-inoculated T. aestivum roots (sample 1)
characteristics.characteristic: title