characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-02-04T17:01:02Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:09:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2341989
characteristics.characteristic: External Id
characteristics.text: TRON-MAINZ
characteristics.characteristic: INSDC center name
characteristics.text: 2014-02-04T17:01:02Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:09:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS399660
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2271:B16-F10 cell line rep3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: melanoma cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0000849
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: B16F10 melanoma cell line was purchased in 2010 from the American Type Culture Collection (product: ATCC CRL-6475, lot number: 58078645). Early (third and fourth) passages of cells were used for tumor experiments. Reauthentification of cells has not been done since receipt. RNA from B16F10 cells was extracted in triplicate. Using 5 ug of total RNA, barcoded mRNA-seq cDNA libraries were prepared in triplicate using a modified Illumina mRNA-seq protocol.
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: B16-F10 cell line rep3
characteristics.characteristic: title