characteristics.text: 2012-07-20T17:00:48Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:37:45Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1466615
characteristics.characteristic: External Id
characteristics.text: Vanderbilt University
characteristics.characteristic: INSDC center name
characteristics.text: 2012-07-20T17:00:48Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:37:45Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS096221
characteristics.characteristic: SRA accession
characteristics.text: Sox17tm1.3(Cre.GFP)Mgn
characteristics.characteristic: StrainOrLine
characteristics.text: E-MTAB-970:E9.5 Sox17^GFP+EpCAM- cells 3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Sox17 expressing endothelial cell in mid gut
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: pool of 26 E9.5 Sox17^GFP/+ embryos Protocols: Embryos were isolated from TgH(Sox17.Cre.GFP)Agb mice; the presence of vaginal plug at noon was considered as E0.5. Embryos were genotyped by fluorescence microscopy. The mid gut areas, including ventral pancreatic buds, were dissected by cutting out on the upper region from the heart and the lower region below the ventral pancreas. The dissection event was confirmed by checking the GFP fluorescence. Tissues were dissociated with Accumax (Sigma) at 37 C. Cells were immuno-labeled with anti-EpCAM (G8.8, Santa Cruz) antibody. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II.
characteristics.characteristic: description
characteristics.text: E9.5
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: E9.5 Sox17^GFP+EpCAM- cells 3
characteristics.characteristic: title