characteristics.text: 2013-11-04T17:01:24Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:28:58Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2075718
characteristics.characteristic: External Id
characteristics.text: Queen's University Belfast
characteristics.characteristic: INSDC center name
characteristics.text: 2013-11-04T17:01:24Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:28:58Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS251232
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1678:N3minusP
characteristics.characteristic: Submitter Id
characteristics.text: 14 day
characteristics.characteristic: age
characteristics.text: 2
characteristics.characteristic: block
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: velvet grass
characteristics.characteristic: common name
characteristics.text: Protocols: Arsenate tolerant and non-tolerant Holcus lanatus L. Genovars were selected randomly from a screened population, the arsenic uncontaminated Cruickshank Botanic Garden (CBG) and tillers of the plants were grown hydroponically for 2 weeks in 50ml individual centrifuge tubes with base of the tubes covered in tin-foil to block out light, in heated glasshouse at around 18C under supplemental sodium lamps. Plant tillers from arsenic tolerant (T) and non-tolerant (N) plants were grown hydroponically for 2 weeks in 50ml complete Hogland solution (plusP treatment) or 50ml Hogland solution minus Phosphate (minusP treatment). The experiment therefore investigated 4 treatments (TplusP, TminusP, NplusP, NminusP) and was repeated 2 time. Experiment1 was conducted on 4 plants (n=1), Experiment2 was conducted on 16 plants (n=4). dry roots and shoots for each replicate were ground under liquid nitrogen and total RNA was extrected using the Plant Rneasy kit (Qiagen), with additional on-column Dnase treatment. Extracted RNA of each sample was stored at -80C until shipping on dry ice for sequencing Illumina TrueSeq RNA sample preparation
characteristics.characteristic: description
characteristics.text: Hogland solution minus phosphate
characteristics.characteristic: growth condition
characteristics.text: N3minusP
characteristics.characteristic: individual
characteristics.text: Holcus lanatus
characteristics.characteristic: organism
characteristics.text: Holcus lanatus L
characteristics.characteristic: organism
characteristics.text: Arsenic-non-tolerant
characteristics.characteristic: phenotype
characteristics.text: Holcus lanatus
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: N3minusP
characteristics.characteristic: title