characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-04-06T17:04:46Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T21:57:28Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3121277
characteristics.characteristic: External Id
characteristics.text: IDIBAPS and IFIBYNE-CONICET
characteristics.characteristic: INSDC center name
characteristics.text: 2015-04-06T17:04:46Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T21:57:28Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS605427
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3061:PB_RNA
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: polyA+ RNA Protocols: Human embryos were collected with informed consent following approval from the North West Regional Ethics Committee, UK (08/H1010/28) following termination of pregnancy and staged immediately by stereomicroscopy according to the Carnegie classification38. The collection, use and storage of material followed guidelines from the UK Polkinghorne Committee, legislation of the Human Tissue Act 2004 and the Codes of Practice of the Human Tissue Authority, UK. The analysis of human embryonic tissue was also approved by the Comitè Ètic d’Investigació Clínica del Centre de Medicina Regenerativa de Barcelona and Departament de Salut, Generalitat de Catalunya. Human embryonic pancreas and liver were dissected at Carnegie Stage (CS) 16-18, which correlates to ~37-45 days post-conception. These stages were the earliest at which the pancreatic epithelial cells could be efficiently dissected away from surrounding mesenchyme with minimal contamination. After isolation, pancreatic and liver tissues were rinsed with phosphate buffered saline 3 times, formaldehyde was added to a final concentration of 1%, and samples were incubated at room temperature for 10 min. Glycine was added to 125 mM for 5 min, tissue was next rinsed 3 times with phosphate-buffered saline containing protease inhibitor cocktail (Roche) at 4ºC, and then snap-frozen and stored at -80ºC. Total embryonic pancreas RNA was extracted from unfixed tissue using Trizol and then treated with DNase I. RNA was extracted usign TRIzol. Total RNA quality was confirmed using Bioanalyzer 2100. All analyzed samples had RIN values greater than 9. RNA-seq for in vivo and in vitro MPCs was generated from DNase I-treated PolyA+ RNA samples from a CS17 pancreatic bud or from a pool of 3 in vitro pancreatic MPC differentiation experiments. Libraries were contructed using Illumina standard recommended procedures.
characteristics.characteristic: description
characteristics.text: Carnegie Stage 17
characteristics.characteristic: developmental stage
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: pancreatic bud
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0003922
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: PB_RNA
characteristics.characteristic: title