characteristics.text: 2012-12-31T17:01:41Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:47:03Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SRA-Webin
characteristics.characteristic: ENA-SUBMISSION-TOOL
characteristics.text: SAMEA2008551
characteristics.characteristic: External Id
characteristics.text: BCCS
characteristics.characteristic: INSDC center name
characteristics.text: 2012-12-31T17:01:41Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:47:03Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS148508
characteristics.characteristic: SRA accession
characteristics.text: RnaSeq
characteristics.characteristic: Submitter Id
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: characteristics = 1-7HB2 cells(Bartek et al. 1991) characteristics = RNA sequencing growth_protocol = HB2 cells (1-7HB2, a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7, (Bartek et al. 1991)) were cultured in DMEM supplemented with 0.2 mM L-glutamine, 100 units/ml penicillin, 100 mg/ml streptomycin, 10% FCS, 5 �g/ml hydroxycortisone and 10 �g/ml human insulin. description = Two microgram of total RNA was converted into a library of template molecules suitable for sequencing according to the Illumina mRNA Sequencing sample prep protocol. Briefly, polyA containing mRNA molecules were purified using poly-T oligo attached magnetic beads. Following purification, the mRNA is fragmented into ~200 bp fragments using divalent cations under elevated temperature. The cleaved RNA fragments are copied into first strand cDNA using reverse transcriptase and random primers. This is followed by second strand synthesis using DNA polymerase I and RNaseH These cDNA fragments are end repaired, a single A base is added and Illumina adaptors are ligated. The products are purified and size selected on gel and enriched by PCR. The PCR products are purified by Qiaquick PCR purification and used for cluster generation according to the Illumina cluster generation protocols (www.illumina.com). The sample was sequenced for 36bp and raw data was processed using Narwhal (Brouwer, R.W., van den Hout, M.C., Grosveld, F.G., and van Ijcken, W.F. 2011. NARWHAL, a primary analysis pipeline for NGS data. Bioinformatics (Oxford, England) 28(2): 284-285.).
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Transcriptome analysis sample
characteristics.characteristic: title