characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-11-17T17:01:09Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:02:58Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2277335
characteristics.characteristic: External Id
characteristics.text: European Molecular Biology Laboratory
characteristics.characteristic: INSDC center name
characteristics.text: 2014-11-17T17:01:09Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:02:58Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS385165
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2123:130814puf3_t40_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: baker's yeast
characteristics.characteristic: common name
characteristics.text: Protocols: Inoculate a 100 ml culture to OD 0.1 from a 5 ml of overnight growth of a single yeast colony in appropriate growth medium. Grow the temperature sensitive strain in permissive temperature (24C) to OD 1.0 and then apply heat shock by addition of hot (65C) growth medium to the growing cells to bring the culture temperature to 37C. Harvest the cells at the required time points after applying the heat shock. To extract total RNA yeast cells were lysed by vortexing with glass beads in 2ml tube with FastPrep. RNA was then cleaned by phenol:chlorophorm extraction followed by ethanol precipitation and DNase treatment. In summary 10 microgrammes of total RNA from budding yeast and 1 microgramme of total RNA from fission yeast was fragmented, purified, retrotranscribed, end-repaired, ligated, amplified and finally size selected. Full protocol is describe in An efficient method for genome-wide polyadenylation site mapping and RNA quantification Wilkening et al., 2013
characteristics.characteristic: description
characteristics.text: puf3 delta
characteristics.characteristic: genotype
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4932
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: scientific_name
characteristics.text: mating type a
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001275
characteristics.characteristic: sex
characteristics.text: Mat a, his3 delta, leu2-3, ura3-52, rpb1::HIS3 with plasmid RY2522
characteristics.characteristic: strain
characteristics.text: 130814puf3_t40_1
characteristics.characteristic: title