characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-04-25T17:00:46Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:16:33Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2401044
characteristics.characteristic: External Id
characteristics.text: University of Oxford
characteristics.characteristic: INSDC center name
characteristics.text: 2014-04-25T17:00:46Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:16:33Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS422908
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2399:primary_monocyte_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: monocyte
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000576
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Monocytes were separated with the Miltenyi Monocyte Isolation kit II (Miltenyi biotec) and re-suspended in RPMI 1640 medium (supp. 2 mM L-glutamine 10,000 U/mL penicillin 10 mg/mL streptomycin and 10% (v/v) fetal bovine serum (Invitrogen, Paisley, UK)) Experimental samples were treated with 10ng/ml LPS for 4 hours and the controls were left untreated (n = 4 per group). Total RNA was extracted using the Qiagen RNeasy kit and included an on-column DNase treatment Ribosomal RNA (rRNA) was depleted using an early-access version of the Ribo-Zero Gold rRNA Removal Kit (Epicentre). Strand-specific cDNA libraries were prepared using an early-access version of the ScriptSeq v2 library preparation kit (Epicentre)
characteristics.characteristic: description
characteristics.text: PeF-NS
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: primary_monocyte_2
characteristics.characteristic: title