characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-03-13T17:00:40Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:15:54Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2397517
characteristics.characteristic: External Id
characteristics.text: University of Pennsylvania, School of Medicine, Dept. of Genetics
characteristics.characteristic: INSDC center name
characteristics.text: 2014-03-13T17:00:40Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:15:54Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS421570
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2350:B63 Dif
characteristics.characteristic: Submitter Id
characteristics.text: 3 month
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000035
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: differentiated intestinal epithelium
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: All procedures involving mice were conducted in accordance with approved Institutional Animal Care and Use Committee protocols. WGSBS was performed as previously published (Lister, Nature 462: 315-322) with the following changes. Isolated genomic DNA was fragmented into 300bp pieces using the S220 Focused - ultrasonicator (Covaris). Sequencing libraries were generated with 500ng genomic DNA using the NEBNext genomic sequencing kit (NEB) and Illumina adaptors (PE-102-1001. PE-102- 1002). Libraries were bisulfite converted using Imprint DNA Modification Kit (Sigma). Size selection of the libraries to 300-600bp was performed on the Pippin Prep DNA size selection system (Sage Science). Amplification of libraries was performed in 18 rounds of PCR using Pfu Turbo Cx Hotstart DNA Polymerase (Agilent Technologies).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: small intestine
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002108
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: C57Bl/6 background Lgr5-EGFP-ires-CreERT2
characteristics.characteristic: strain
characteristics.text: B63 Dif
characteristics.characteristic: title