characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-02-06T17:01:43Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T20:07:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2658264
characteristics.characteristic: External Id
characteristics.text: EBI
characteristics.characteristic: INSDC center name
characteristics.text: 2015-02-06T17:01:43Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T20:07:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS508292
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2762:Sample 4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Silastic tubing containing 35 ul of oil or 35ul of oil with 180ug/ml E2. Implants remained in mice for 7 days. Total RNA was extracted using Ambion RNAqueous according to manufacturers protocol.Samples were treated with Turbo DNA-free reagent to remove genomic DNA. The TruSeq RNA sample prep kit was used according to manufacturers protocol. 1ug total RNA was used to make poly-A selected and barcoded libraries. Libraries were pooled and quantifed using real time PCR and library quantification kit (Kapa Biosystems).
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: Sample 4
characteristics.characteristic: title