characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-01-06T17:01:52Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T19:42:31Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2636343
characteristics.characteristic: External Id
characteristics.text: EBI
characteristics.characteristic: INSDC center name
characteristics.text: 2016-01-06T17:01:52Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T19:42:31Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS502443
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2643:iPS-j
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: induced pluripotent stem cell
characteristics.characteristic: cell type
characteristics.text: pig
characteristics.characteristic: common name
characteristics.text: LIF dependent pig iPS in mouse ESC Protocols: The piPS cells were maintained on mitotically inactive mouse embryonic fibroblasts (MEFs) feeder derived from ICR mice supplemented with 10% FBS, sodium pyruvate, nonessential amino acids, mercaptoethanol, and LIF, bFGF. For immunoprecipitation, the DNA was first denatured, then immunoprecipitated with the 5mC antibody using the Magnetic Methylated DNA Immunoprecipitation kit (Diagenod). Genomic DNA was extracted from the cell pellets using the DNeasy Mini Kit (Qiagen). The gDNA was sonicated to 100-500 bp and repaired to contain a 3-dA overhang; adapters were then ligated to the end of the DNA fragments according to the Paired-End DNA Sample Prep Kit (Illumina). Real time-PCR was performed to validate the enrichment efficiency. Next, the immunoprecipitated DNA was amplified for approximately 12-15 cycles, and fragments of the proper size (usually 200-300 bp) were gel-purified using the Gel Extraction Kit (Qiagen) and quantified using a 2100 Bioanalyzer (Agilent Technologies).
characteristics.characteristic: description
characteristics.text: media
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000579
characteristics.characteristic: growth condition
characteristics.text: Sus scrofa
characteristics.characteristic: organism
characteristics.text: Sus scrofa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9823
characteristics.characteristic: organism
characteristics.text: Sus scrofa
characteristics.characteristic: scientific_name
characteristics.text: iPS-j
characteristics.characteristic: title