characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-05-31T17:01:37Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T18:34:09Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2603465
characteristics.characteristic: External Id
characteristics.text: Max Planck Institute for Molecular Genetics, OWL Epigenomics
characteristics.characteristic: INSDC center name
characteristics.text: 2016-05-31T17:01:37Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T18:34:09Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS481525
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2637:RNA-seq PHF13 knockdown
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: ES-E14 cell
characteristics.characteristic: cell line
characteristics.text: embryonic stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002322
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: mESCs were grown in Glasgow Minimum Essential medium supplemented with 20% FBS, 1% Glutamax, 1% Penicillin/Streptomyocin, 1% Non-Essential aminoacids, 1% Sodium Pyruvate and 0.2% _-mecaptoethanol. The medium was supplemented with LIF. The cells were maintained at sub confluent amounts and were grown at 37 degree celsius and 5% CO2. The knockdown was done with mouse SPOC1 (Phf13) or scrambled shRNA lenti-viruses purchased from Sigma (Mission NM_172705). Total cellular RNA was collected using RNeasy plus mini-kit (Qiagen) according to manufactures instructions. 1 microgram of total RNA was then depleted of ribosomal RNA using the Ribo zero magnetic kit (Epicenter) according to the manufacturer's directions. The rRNA depleted RNA was then measured by Qubit and 50ng was used to generate the RNA library using ScriptSeq v2 RNA-Seq library preparation kit (Epicenter) according to the manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: RNA-seq PHF13 knockdown
characteristics.characteristic: title