characteristics.text: 2012-09-24T17:00:57Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:38:24Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1523614
characteristics.characteristic: External Id
characteristics.text: MICHAEL SMITH GENOME SCIENCES CENTRE, VANCOUVER, CANADA
characteristics.characteristic: INSDC center name
characteristics.text: 2012-09-24T17:00:57Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:38:24Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS098276
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-971:CB541
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: primary cell line
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0003064
characteristics.characteristic: cell line
characteristics.text: neuronal stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000047
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: GNS and NS cells were cultured in N2B27 serum-free medium, a 1:1 mixture of DMEM/F-12 and Neurobasal media (Invitrogen) augmented with N2 (Stem Cell Sciences) and B27 (Gibco) supplements. Self-renewal was supported by the addition of 10 ng/ml EGF and 20 ng/ml FGF-2 to the complete medium. Cells were plated at 20,000/cm2 in laminin-coated vessels (10 ug/ml laminin-1 (Sigma) in PBS for 6-12 h), passaged at confluence using Accutase dissociation reagent (Sigma) and were typically split at 1:3 for NS cells and 1:3 to 1:6 for GNS cells. TRIzol, phenol/chloroform extraction
characteristics.characteristic: description
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease state
characteristics.text: CB541
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: cerebral cortex
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000956
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: CB541
characteristics.characteristic: title