characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-12-17T17:02:18Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:25:20Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2472063
characteristics.characteristic: External Id
characteristics.text: Department of Entomology, Nanjing Agricultural University
characteristics.characteristic: INSDC center name
characteristics.text: 2014-12-17T17:02:18Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:25:20Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS441526
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2491:Turt_FI
characteristics.characteristic: Submitter Id
characteristics.text: 1 to 3 day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: two-spotted spider mite
characteristics.characteristic: common name
characteristics.text: Protocols: Mites used in this study were reared on a leaf of the common bean (Phaseolus vulgaris L.) placed on a water-saturated sponge mat in Petri dishes (dia. 9) at 25 degrees C, 60% r. h. and under L16-D8 conditions. Both 100% Wolbachia infected and 100% uninfected Wolbachia lines were established. RNA was extracted using the Trizol protocol Poly (A) mRNA was isolated with oligo-dT beads and then treated with the fragmentation buffer. The cleaved RNA fragments were then transcribed into first-strand cDNA using reverse transcriptase and random hexamer primers. This was followed by second strand cDNA synthesis using DNA polymerase I and RNaseH. The double stranded cDNA was further subjected to end-repair using T4 DNA polymerase, Klenow fragment, and T4 Polynucleotide kinase followed by a single A base addition using Klenow 3' to 5' exo-polymerase. It was then ligated with adapter or index adapter using T4 quick DNA ligase. Adaptor ligated fragments were selected according to the size and the desired range of cDNA fragments were excised from the gel. PCR was performed to selectively enrich and amplify the fragments. Finally, after validating on an Agilent 2100 Bioanalyzer and ABI Step One plus Real-Time PCR System, the cDNA library was sequenced on a flow cell using Illumina HiSeq2000.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Wolbachia infected
characteristics.characteristic: infect
characteristics.text: Tetranychus urticae
characteristics.characteristic: organism
characteristics.text: Tetranychus urticae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_32264
characteristics.characteristic: organism
characteristics.text: Fecundity increase
characteristics.characteristic: phenotype
characteristics.text: Tetranychus urticae
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: Turt_FI
characteristics.characteristic: title