characteristics.text: 2013-03-29T17:01:40Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:20:53Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1714538
characteristics.characteristic: External Id
characteristics.text: Institute for Systems Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2013-03-29T17:01:40Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:20:53Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS226501
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1568:SM1_crc1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: The plasma was prepared from EDTA blood by centrifugation at 1,0006g for 15 minutes to separate the plasma and blood cells. Total RNA from plasma was extracted from 100 ml of the sample using the miRNeasy kit (Qiagen, Valencia, CA). The quality and quantity of the RNA were evaluated with Agilent 2100 Bioanalyzer (Santa Clara, CA) and NanoDrop 1000 spectrophotometer (Thermo Scientific, Wilmington, DE). In average, we obtained about 100 ng of RNA per ml of plasma. As a control we also obtained total RNA from Ambion (Life Technologies, Carlsbad, CA). NextGen sequencing libraries were prepared with small RNA sample preparation kits from Illumina (Illumina, San Diego, CA).
characteristics.characteristic: description
characteristics.text: colorectal cancer
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005842
characteristics.characteristic: disease state
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: plasma
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001969
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: SM1_crc1
characteristics.characteristic: title