characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2013-11-30T17:01:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:51:27Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2237187
characteristics.characteristic: External Id
characteristics.text: Functional Genomics Group, PICB
characteristics.characteristic: INSDC center name
characteristics.text: 2013-11-30T17:01:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:51:27Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS366000
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2008:siGata3_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: PC12
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001225
characteristics.characteristic: cell line
characteristics.text: Norway rat
characteristics.characteristic: common name
characteristics.text: Protocols: PC12 cells were plated on a Poly-L-lysine-coated dishes (Corning) and maintained in DMEM/F12 (Invitrogen) with 5% FBS(Biochrom), 5% horse serum (Gibco) and 1% penicillin/streptomycin at 37C in 5% CO2. We used two different custom-made siRNAs against Gata3. siGATA3-1 (sense 5-GUACUACAAACUCCACAAUTT-3 and antisense 5-AUUGUGGAGUUU GUAGUACTT-3), siGATA3-2 (sense 5-CCGUAAGAUGUCUAGCAAATT-3 and antisense 5-UUUGCUAGACAUCUUACGGTT3) and negative controls (sense 5-UUCUCCGAACGUGUCACGUTT-3 and antisense 5-ACGUGAC ACGUUCGGAGAATT) were obtained from GenePharma (Shanghai). All siRNA experiments were at a final concentration of 50nM. Transfections were conducted using Lipofectamine RNAiMAX (Invitrogen). Total RNA was isolated from cells using Trizol (Invitrogen).
characteristics.characteristic: description
characteristics.text: Rattus norvegicus
characteristics.characteristic: organism
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: Rattus norvegicus
characteristics.characteristic: scientific_name
characteristics.text: siGata3_2
characteristics.characteristic: title