characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2016-01-21T17:01:32Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:20:57Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2729906
characteristics.characteristic: External Id
characteristics.text: School of Agriculture, Policy and Development, The University of Reading, UK
characteristics.characteristic: INSDC center name
characteristics.text: 2016-01-21T17:01:32Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:20:57Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS433765
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2465:WT_L
characteristics.characteristic: Submitter Id
characteristics.text: 4 to 6 week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Leaf tissues collected from wild type plants (N7000) Protocols: Seeds were germinated and grown on vermiculite containing soil and maintained at 25oC in 60% Relative Humidity with a day-night cycle of 16/8 h light/dark in controlled environmental conditions. Total RNA was extracted using the RNeasy® Plant Mini Kit (Qiagen, UK) according to the manufacturer’s protocol. The cDNA libraries were constructed in accordance with the Illumina TruSeq RNA sample preparation guide v2 for Illumina paired-end multiplexed sequencing by Source Bioscience, UK.
characteristics.characteristic: description
characteristics.text: floral transition
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002683
characteristics.characteristic: developmental stage
characteristics.text: Columbia-0
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005148
characteristics.characteristic: ecotype
characteristics.text: No modification
characteristics.characteristic: genetic modification
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: WT_L
characteristics.characteristic: title