characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-12-19T17:02:04Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T23:18:53Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3205989
characteristics.characteristic: External Id
characteristics.text: European Molecular Biology Laboratory
characteristics.characteristic: INSDC center name
characteristics.text: 2015-12-19T17:02:04Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T23:18:53Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS638368
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3195:ZIP_hSCrud_Input_not1d_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: baker's yeast
characteristics.characteristic: common name
characteristics.text: Protocols: Grow single colony overnight in 5ml YPD at 30C unless the growth media is otherwise specified in sample annotations. Dilute in 50-100ml desired medium with starting OD600=0.1. Grow to OD600=1.0. Harvest cells for total RNA extraction. The exponentially growing cells were harvested at OD 0.6-0.9and broken with glass beads. An aliquot of lysate was taken as Input control, the remaining was subjected to immunoprecipitation (IP) with Dyna M280 sheep anti rabbit IgG for 2h at 4??C and analyzed as described previously (Gupta et al., 2014). Negative control IP was performed following the above procedure on a BY4741 strain without a Tap-tag on any protein. In summary 10 ??g of total RNA was fragmented, purified, retrotranscribed, end-repaired, ligated, amplified and finally size selected. Full protocol is describe in An efficient method for genome-wide polyadenylation site mapping and RNA quantification Wilkening et al., 2013
characteristics.characteristic: description
characteristics.text: gene knock out
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000506
characteristics.characteristic: genetic modification
characteristics.text: not5_Deletion
characteristics.characteristic: genotype
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4932
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: scientific_name
characteristics.text: MAT_ leu2_20 ura3_met15_ lys2_ his3delta1 not1::NOT1-Tap-tag-URA3
characteristics.characteristic: strain
characteristics.text: ZIP_hSCrud_Input_not1d_1
characteristics.characteristic: title