characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-11-11T17:02:10Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T20:51:07Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3112881
characteristics.characteristic: External Id
characteristics.text: MDC
characteristics.characteristic: INSDC center name
characteristics.text: 2014-11-11T17:02:10Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T20:51:07Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS580102
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2572:raw_rbm20_hits_NH_HM_002
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: myocyte
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000187
characteristics.characteristic: cell type
characteristics.text: Norway rat
characteristics.characteristic: common name
characteristics.text: Protocols: Neonatal rat ventricular myocytes (NRVMs) were isolated from SD rats using a modification of the Kasten's technique as described (37). 48 h past isolation, cardiomyocytes were irradiated one time for 400mJ/cm and additional 200 mJ/cm with 254 nm UV light, and lysed in NP40 lysis buffer. After DNAse and RNase T1 treatment, the lysate was immunoprecipitated using RBM20 antibody (5) covalently coupled to Protein G Dynabeads (Invitrogen). Radiolabeling of immunoprecipitated RNA, protein-RNA complexes separation, sequencing library preparation and sequencing was done following the PAR-CLIP protocol 4 ug of total RNA was used for library preparation with the TruSeq RNA Sample Preparation Kit (Illumina).
characteristics.characteristic: description
characteristics.text: HITS-CLIP 2
characteristics.characteristic: individual
characteristics.text: Rattus norvegicus
characteristics.characteristic: organism
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: Rattus norvegicus
characteristics.characteristic: scientific_name
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: raw_rbm20_hits_NH_HM_002
characteristics.characteristic: title