characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-01-29T17:02:59Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T18:50:00Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2677897
characteristics.characteristic: External Id
characteristics.text: Cambridge Institute - Cancer Research UK and University of Cambridge
characteristics.characteristic: INSDC center name
characteristics.text: 2015-01-29T17:02:59Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T18:50:00Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS517788
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2633_rev:Opossum13
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: gray short-tailed opossum
characteristics.characteristic: common name
characteristics.text: Protocols: For fresh specimens, hepatocytes were prepared by direct perfusion of the liver with buffered salt solution, followed by cross-linking of the diced tissue in 1% formaldehyde solution for 20 minutes, addition of 250 mM glycine and incubation for a further After homogenization of cross-linked liver tissue in a dounce tissue grinder, hepatocytes were rinsed with PBS and lysed according to published protocols {Schmidt, 2009} to solubilize DNA-protein complexes. Chromatin was fragmented to 300 bp average size 1) Immunoprecipitation. Chromatin from 0.1 g of dounced liver tissue was used for each ChIP experiment using antibodies against H3K4me3 (millipore 05-1339) and H3K27Ac (abcam ab4729) and automated 96-well protocols in an Agilent Bravo liquid handling rob
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Opossum13
characteristics.characteristic: individual
characteristics.text: Monodelphis domestica
characteristics.characteristic: organism
characteristics.text: Monodelphis domestica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_13616
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Monodelphis domestica
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: Opossum13
characteristics.characteristic: title