characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-12-19T17:02:04Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T23:17:50Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3205983
characteristics.characteristic: External Id
characteristics.text: European Molecular Biology Laboratory
characteristics.characteristic: INSDC center name
characteristics.text: 2015-12-19T17:02:04Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T23:17:50Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS638362
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3195:ZIP_polzm_Polzm_TE_Not1TT_wt_15
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: baker's yeast
characteristics.characteristic: common name
characteristics.text: Protocols: Grow single colony overnight in 5ml YPD at 30C unless the growth media is otherwise specified in sample annotations. Dilute in 50-100ml desired medium with starting OD600=0.1. Grow to OD600=1.0. Harvest cells for total RNA extraction. The polysome fraction was profiled henceforth was done as described in (Albanese et al., 2010). Briefly , 400 ml yeast in exponential growth phase was treated with 100 ??g/ml cycloheximide, harvested, washed with cold lysis buffer, resuspended in 1 ml of buffer A (20 mM Hepes, 50 mM KCl, 10 mM MgCl2, 1% Triton X-100, 1 mM DTT, and protease inhibitor cocktail), and frozen as drops in liquid nitrogen. The cells were ground and 1 ml of buffer A was added to the powder, and the lysates were clarified by centrifugation at 14,000 g for 10 min. 20 OD lysate was applied on a 12 ml 7???47% sucrose gradient in buffer A and centrifuged in a rotor (SW41; Beckman Coulter) for 150 min at 39,000 rpm at 4??C. Fractions were collected using a UA/6 detector (ISCO, Inc.). When indicated, polysomes were dissociated by treatment with 25 mM EDTA. The fractions were TCA precipitated, separated by SDS-PAGE, and subjected to immunoblot analysis. In summary 10 ??g of total RNA was fragmented, purified, retrotranscribed, end-repaired, ligated, amplified and finally size selected. Full protocol is describe in An efficient method for genome-wide polyadenylation site mapping and RNA quantification Wilkening et al., 2013
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4932
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: scientific_name
characteristics.text: MAT_ leu2_20 ura3_met15_ lys2_ his3delta1 not1::NOT1-Tap-tag-URA3
characteristics.characteristic: strain
characteristics.text: ZIP_polzm_Polzm_TE_Not1TT_wt_15
characteristics.characteristic: title