characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-05-12T17:01:54Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T19:51:37Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2672869
characteristics.characteristic: External Id
characteristics.text: University Medicine Greifswald
characteristics.characteristic: INSDC center name
characteristics.text: 2015-05-12T17:01:54Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T19:51:37Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS513845
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2789:120727_DMSO
characteristics.characteristic: Submitter Id
characteristics.text: 8 week
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000034
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: dorsal root ganglia
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000044
characteristics.characteristic: cell type
characteristics.text: Norway rat
characteristics.characteristic: common name
characteristics.text: Protocols: Lumbar dorsal root ganglia were isolated from 8 weeks old Spargue Dawley rats Doral root ganglia were isolated from three rats were split into half and treated with solvent DMSO (0.1%) or RTX (100 nM) for 30 min. Three replicate experiments were performed at the same time on different days resulting in 6 samples (2 samples each from 3-rat pools '120726', '120727' and '120730'). For the RTX treatment, dorsal root ganglia were incubated in 5 ml MEM containing collagenase P (Roche, Penzberg, DE) (0.1 U/ml, 1 h, 37 degrees C, 5% CO2), dissociated by trituration with fire-polished Pasteur pipettes, and then treated with the solvent DMSO (0.1%) or RTX (100 nM). The cells were spun down (100 g, 5 min), resuspended in 1 ml Hank's buffered salt solution (pH 8.0) containing 0.025% EDTA and 245 U/ml trypsin (Worthington, Lakewood, NJ), and incubated for 4 min at 37 degrees C in a water bath. Trypsination was stopped by the addition MgSO4 (400 uM final conc.). The cell suspension was then loaded onto BSA gradients (15% BSA in Hank's buffered salt solution) and centrifuged (120 g, 8 min). The pellet was frozen in liquid nitrogen and stored at -80 degrees C for RNA isolation. RNA isolation was performed with NucleoSpin RNA/Protein kits (Macherey-Nagel, Dueren, Germany) including on column DNase treatment. Ribosomal RNA was removed from 5 ug total RNA per sample using Ribo-Zero rRNA Removal Kit (Epicentre Biotechnologies, Madison, WI, USA).
characteristics.characteristic: description
characteristics.text: Rattus norvegicus
characteristics.characteristic: organism
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: Rattus norvegicus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Sprague Dawley
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001352
characteristics.characteristic: strain
characteristics.text: 120727_DMSO
characteristics.characteristic: title