characteristics.text: GSC:MIxS v2.1
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2012-01-10T15:58:43Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: GSC, MIxS
characteristics.characteristic: ENA-KEYWORD
characteristics.text: 2018-03-09T09:55:25Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1094722
characteristics.characteristic: External Id
characteristics.text: Germany, Lower Saxony
characteristics.characteristic: Geographic location (country:region,area)
characteristics.text: VTI
characteristics.characteristic: INSDC center name
characteristics.text: 2012-01-10T15:58:43Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-09T09:55:25Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS084151
characteristics.characteristic: SRA accession
characteristics.text: 3BT_b
characteristics.characteristic: Submitter Id
characteristics.text: BBCH 63 - 65; August 2009
characteristics.characteristic: collection date
characteristics.text: maize culticar 3BT
characteristics.characteristic: current vegetation
characteristics.text: 2-15 cm
characteristics.unit: m
characteristics.characteristic: depth
characteristics.text: Rhizosphere microbial communities were collected by saline washes of fine roots (diameter > mm) of maize cultivar 3BT, collected at the flowering stage BBCH 63-65. Individual plants were sampled. Total DNA was extracted from the collected cells by “FastDNA SPIN kit for soil” (MP Biomedicals, Illkirch, France). The extraction included two bead beating steps of 45 s at 6.5 m s-1 on a FastPrep-24 system (MP Biomedicals) and additional washing steps of the binding matrix each with 1 ml 5.5 M guanidine thiocyanate (Carl Roth GmbH) until the matrix retained its original color (1 - 3 washes).
characteristics.characteristic: description
characteristics.text: soil
characteristics.characteristic: enviornmental package
characteristics.text: field soil; ENVO:00005755
characteristics.characteristic: environment (featurel)
characteristics.text: rhizosphere; ENVO:00005801
characteristics.characteristic: environment (material)
characteristics.text: 52° N
characteristics.characteristic: geographic location (latitude)
characteristics.text: 10° E
characteristics.characteristic: geographic location (longitude)
characteristics.text: MIMARKS
characteristics.characteristic: investigation type
characteristics.text: “FastDNA SPIN kit for soil” (MP Biomedicals, Illkirch, France)
characteristics.characteristic: nucleic acid extraction
characteristics.text: rhizosphere metagenome
characteristics.characteristic: organism
characteristics.text: initial denaturation: 95degC_15min; 30 cycles of: denaturation: 94degC_60s, annealing: 50degC_60s, extention: 72degC_70s; final extension at 72degC_5min
characteristics.characteristic: pcr conditions
characteristics.text: F_Com1_MID9: ccatctcatccctgcgtgtctccgactcagTAGTATCAGCCAGCAGCCGCGGTAATAC; R_Com2: cctatcccctgtgtgccttggcagtctcagCCGTCAATTCCTTTGAGTTT
characteristics.characteristic: pcr primers
characteristics.text: 6.1
characteristics.characteristic: ph
characteristics.text: maize rhizosphere
characteristics.characteristic: project name
characteristics.text: rhizosphere metagenome
characteristics.characteristic: scientific_name
characteristics.text: Pyrosequencing
characteristics.characteristic: sequencing method
characteristics.text: 16S rRNA
characteristics.characteristic: target gene
characteristics.text: Rhizosphere microbial communities were collected by saline washes of fine roots (diameter > mm) of maize cultivar 3BT, collected at the flowering stage BBCH 63-65. Individual plants were sampled. Total DNA was extracted from the collected cells by “FastDNA SPIN kit for soil” (MP Biomedicals, Illkirch, France). The extraction included two bead beating steps of 45 s at 6.5 m s-1 on a FastPrep-24 system (MP Biomedicals) and additional washing steps of the binding matrix each with 1 ml 5.5 M guanidine thiocyanate (Carl Roth GmbH) until the matrix retained its original color (1 - 3 washes).
characteristics.characteristic: title