characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-07-01T17:01:00Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:49:25Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2223458
characteristics.characteristic: External Id
characteristics.text: Vanderbilt University
characteristics.characteristic: INSDC center name
characteristics.text: 2014-07-01T17:01:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:49:25Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS359844
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1929:E15.5 Insm1^EGFP/Pdx1^CFP embryos 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: pool of 4 E15.5 Insm1^EGFP/Pdx1^CFP Het/Het embryos Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Heterozygous embryos were identified using a fluorescent microscope, and embryos obtained from heterozygous interbreeding were genotyped using custom-designed TaqMan primers and probes. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II.
characteristics.characteristic: description
characteristics.text: embryonic day 15.5
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002566
characteristics.characteristic: embryo stage
characteristics.text: embryo stage
characteristics.characteristic: life cycle stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Insm1tm2(GFP.Cre)Mgn/Pdx1tm2(CFP)Mgn mouse strain
characteristics.characteristic: strain
characteristics.text: E15.5 Insm1^EGFP/Pdx1^CFP embryos 1
characteristics.characteristic: title