characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2013-09-01T17:01:55Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:41:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2171063
characteristics.characteristic: External Id
characteristics.text: Department of Genetics, Evolution, and Environment & Cancer Institute, University College London
characteristics.characteristic: INSDC center name
characteristics.text: 2013-09-01T17:01:55Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:41:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: 1 hour
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000032
characteristics.characteristic: PAT cells culture time
characteristics.text: 7 hour
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000032
characteristics.characteristic: PAT cells culture time
characteristics.text: 2 hour
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000032
characteristics.characteristic: PAT cells culture time
characteristics.text: 6 hour
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000032
characteristics.characteristic: PAT cells culture time
characteristics.text: 0 hour
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000032
characteristics.characteristic: PAT cells culture time
characteristics.text: 3 hour
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000032
characteristics.characteristic: PAT cells culture time
characteristics.text: 5 hour
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000032
characteristics.characteristic: PAT cells culture time
characteristics.text: 4 hour
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000032
characteristics.characteristic: PAT cells culture time
characteristics.text: 8 hour
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000032
characteristics.characteristic: PAT cells culture time
characteristics.text: ERS339378
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1824:pooled_PAT_cells
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fission yeast
characteristics.characteristic: common name
characteristics.text: Protocols: Fission yeast pat1-114 cells (ade6-M210/ade-M216 pat1-114/pat1-114 h+/h+) were grown to mid-log phase at 25 degree C in Edinburgh Minimal Medium (EMM). Cells were washed twice in EMM without nitrogen source (EMM-N) and cultured for 12h in EMM-N at 25 degree C. The culture was then supplemented with 0.5g/L NH4Cl and shifted to 34 degree C. Samples were collected for RNA preparation just before, and every hour for 8 hours after shifting the culture to 34 degree C. Equal RNA amounts from each time-point were pooled and used for sequencing library preparation. Total RNA was isolated from S. pombe cells using a hot phenol method followed by phenol-chloroform extractions, and precipitation as described (Lyne et al., 2003, BMC Genomics 4, 27.). Equal amounts of RNA from each time point were pooled together. 10ug total RNA was used as starting material for strand-specific RNA-seq library preparation using an unreleased early version provided by Illumina of the Illumina TruSeq Small RNA Sample Prep Kit. Briefly: poly(A)+ RNA was enriched by two rounds of poly(dT) Sera-Mag magnetic beads purification. Enriched poly(A)+ RNA was then fragmented to an average size of ~200nt. Fragmented RNA was 3' de-phosphorylated with Antartic phosphatase and 5' phosphorylated with polynucleotide kinase this treatment prepares RNA fragments for subsequent ligation of Illumina RNA adaptors to their 5' and 3' ends using a 3' RNA ligase and a T4 RNA ligase, respectively. First strand cDNA was produced using a primer specific for the Illumina 3' adaptor. The library was finally amplified with 15 cycles of PCR using primers specific for the Illumina adaptors and purified using SPRI-beads (Agencourt, Beckman Coulter). Libraries size distributions and concentrations were determined on an Agilent Bioanalyzer
characteristics.characteristic: description
characteristics.text: ade6-M210/ade-M216 pat1-114/pat1-114 h+/h+
characteristics.characteristic: genotype
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4896
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: scientific_name
characteristics.text: mating type h plus
characteristics.characteristic: sex
characteristics.text: pat1-114
characteristics.characteristic: strain
characteristics.text: pooled_PAT_cells
characteristics.characteristic: title