characteristics.text: 2012-07-20T17:00:48Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:37:17Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1466760
characteristics.characteristic: External Id
characteristics.text: CRUK-CRI
characteristics.characteristic: INSDC center name
characteristics.text: 2012-07-20T17:00:48Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:37:17Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS100989
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1008:iCLIP_Nova_mm_Brain_20100320_LUjn01_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mouse brain tissue (Nova iCLIP) or COS-7 cells grown in a tissue culture plate (mutant NSUN2 iCLIP). Tissue was dissociated in ice-cold PBS buffer (Nova iCLIP) or medium was replaced (mutant NSUN2 iCLIP). Material was then subjected to UV-C irradiation. Cells were then precipitated by centrifugation for 1 min at 14000 rpm and snap-frozen on dry ice. iCLIP protocol was followed to isolate RNA bound to Nova protein or mutant NSUN2 protein (C271A) and amplify cDNA library as described in the manuscript. Specific barcodes were used for each RT reaction, and are specified below for each sequencing run. Random barcodes were also incoperated into cDNAs to distinguish between PCR duplication of cDNA products. The final products were obtained by PCR with Illumina pair-end sequencing primers: 5-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3; 5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: iCLIP_Nova_mm_Brain_20100320_LUjn01_2
characteristics.characteristic: title