characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-01-14T17:01:32Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T18:24:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2618357
characteristics.characteristic: External Id
characteristics.text: Department of Entomology, Nanjing Agricultural University
characteristics.characteristic: INSDC center name
characteristics.text: 2015-01-14T17:01:32Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T18:24:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS487722
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2606:MU
characteristics.characteristic: Submitter Id
characteristics.text: 1 day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: two-spotted spider mite
characteristics.characteristic: common name
characteristics.text: Protocols: Mites used in this study were reared on a leafleaves of the common bean (Phaseolus vulgaris L.) placed on a water-saturated sponge mat in Petri dishes (dia. 9) at 25 degree Celsius, 60 percent relative humility. and under L16-D8 conditions. Both Wolbachia infected and uninfected Wolbachia lines were established. RNA was extracted using the Trizol protocol A total amount of 3 microgramme total RNA per sample was used as input material for the small RNA library. Sequencing libraries were generated using TruSeq small RNA Sample Pre Kit (Illumina) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. Briefly, 3' ends adaptor and 5' ends adaptor were successively ligated to 3' end and 5' end of the RNA sample. Reverse transcription followed by PCR is used to create cDNA constructs based on the small RNA ligated with 3? and 5? adapters. PCR products were purified on a 6% polyacrylamide gel (145V, 60 min). DNA fragments corresponding to 130~160 bp (the length of small noncoding RNA plus the 3' and 5' adaptors) were recovered and dissolved in 8 microlitre elution buffer. At last, library quality was assessed on the Agilent Bioanalyzer 2100 system using DNA High Sensitivity Chips. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq SR Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq 2000 platform and 50bp single-end reads were generated (Novogene Experimental Department).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: uninfected
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001460
characteristics.characteristic: infect
characteristics.text: Tetranychus urticae
characteristics.characteristic: organism
characteristics.text: Tetranychus urticae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_32264
characteristics.characteristic: organism
characteristics.text: Normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: phenotype
characteristics.text: Tetranychus urticae
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: MU
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10088
characteristics.characteristic: title