characteristics.text: 2013-01-01T17:01:46Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:01:18Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1691812
characteristics.characteristic: External Id
characteristics.text: CRUK Cambridge Research Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2013-01-01T17:01:46Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:01:18Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS140919
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1147:HNRNPC knockdown 1 (KD1) - Replicate 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HeLa
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001185
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: HeLa cells were grown in Dulbeccos modified Eagle medium (DMEM) supplemented with 10% Fetal Bovine Serum and 1% penicillin-streptomycin and cultured at 37C with 5% CO2. For the knockdown of HNRNPC, HeLa cells were plated at concentration of 105 cells/well in a 6-well cluster plate in 2 ml of growth medium. 24 h after the plating (when confluency reached around 20%), cultures were independently transfected using two different hnRNP C Stealth Select RNAi siRNAs (KD1 and KD2 refer to siRNAs HSS179304 and HSS179305 from Invitrogen, respectively) at a final concentration of 5 nM. Both siRNAs were transfected using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturers instructions. Using the same procedure, we used Stealth RNAi siRNA Negative Control (Invitrogen) for the control experiments. Library preparation was preformed according to mRNA Sequencing Sample Preparation Guide (Illumina, part # 1004898 REV. D). Reagents were taken from the Illumina sample preparation kit (Illumina, CAT # RS-930-1001).
characteristics.characteristic: description
characteristics.text: HNRNPC knockdown with siRNA1
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: HNRNPC knockdown 1 (KD1) - Replicate 1
characteristics.characteristic: title