characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-08-18T17:01:09Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:19:07Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2431827
characteristics.characteristic: External Id
characteristics.text: Universite Joseph Fourier Grenoble
characteristics.characteristic: INSDC center name
characteristics.text: 2014-08-18T17:01:09Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:19:07Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS427985
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2444:Liver_BAP_24h
characteristics.characteristic: Submitter Id
characteristics.text: 5 month
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000035
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: western clawed frog
characteristics.characteristic: common name
characteristics.text: Protocols: Xenopus (X. tropicalis) females were purchased from the Xenopus national husbandry (CRB Xenopes), University of Rennes 1 (http://xenopus.univ-rennes1.fr/). The frogs were fed daily ad libitum with pellets of trout food. They were allowed to acclimatize for 3 weeks at 25ﾰC with a photoperiod of 12h:12h in constantly filtered water prior to starting the experiments Xenopus were exposed individually at 25ﾰC in 1 L glass jars protected from the light (3 individual replicates by times and exposure conditions). The exposure solutions consisted of 500 mL tap water (pH 7.8, organic carbon = 0.6 mg L-1, dissolved oxygen = 9.9 mg L-1, nitrate = 2.9 mg L-1, nitrites < 0.02 mg L-1) containing BaP at an initial concentration of 10 ﾵg L-1. Control frogs were exposed to ethanol (vehicle) at 1/1000 (v:v) concentration. To avoid circadian effects, experiments with control and BaP-exposed animals were conducted in parallel. The frogs were not fed during the exposure period (from 0 to 24h) For each biological replicate, total RNA was extracted from 15 mg liver using the RNAqueousﾮ-4PCR Kit (Ambion, USA) according to manufacturer's instructions. Total RNA quality and quantity were controlled on an Agilent 2100 Bioanalyzer (Agilent, USA). After extraction, total RNA from each biological replicate were equally pooled in order to obtain 8ﾵg of total RNA in 50ﾵl for each treatment and used for mRNAseq banks preparations using mRNA-Seq-8 Sample Prep Kit (Illumina, USA) according to manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: adults
characteristics.characteristic: developmental stage
characteristics.text: Xenopus (Silurana) tropicalis
characteristics.characteristic: organism
characteristics.text: Xenopus tropicalis
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_8364
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Xenopus (Silurana) tropicalis
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: fresh_specimen
characteristics.characteristic: specimen with known storage state
characteristics.text: Liver_BAP_24h
characteristics.characteristic: title