characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2013-11-30T17:01:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:51:27Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2237190
characteristics.characteristic: External Id
characteristics.text: Functional Genomics Group, PICB
characteristics.characteristic: INSDC center name
characteristics.text: 2013-11-30T17:01:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:51:27Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS366003
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2008:Gata3_ChIP_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: PC12
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001225
characteristics.characteristic: cell line
characteristics.text: Norway rat
characteristics.characteristic: common name
characteristics.text: Protocols: PC12 cells were plated on a Poly-L-lysine-coated dishes (Corning) and maintained in DMEM/F12 (Invitrogen) with 5% FBS(Biochrom), 5% horse serum (Gibco) and 1% penicillin/streptomycin at 37C in 5% CO2. Cells were cross-linked with formaldehyde and sonicated to generate chromatin fragments size enriches between 200-600 bp. Antibody against GATA3 (558686, BD PharmingenTM ) was used. Chromatin from 20 million cells was used for each ChIP experiment, which yielded approximately 10 ng of DNA. As input, 2% of sonicated chromatin was treated with proteinase K at 50C for 2 hr and purified using the QIAquick PCR Purification Kit (Qiagen Cat # 28106).
characteristics.characteristic: description
characteristics.text: Rattus norvegicus
characteristics.characteristic: organism
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: Rattus norvegicus
characteristics.characteristic: scientific_name
characteristics.text: Gata3_ChIP_2
characteristics.characteristic: title