characteristics.text: 2012-06-19T14:37:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:47:28Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SRA-Webin
characteristics.characteristic: ENA-SUBMISSION-TOOL
characteristics.text: SAMEA1468522
characteristics.characteristic: External Id
characteristics.text: BCCS
characteristics.characteristic: INSDC center name
characteristics.text: 2012-06-19T14:37:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:47:28Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS148812
characteristics.characteristic: SRA accession
characteristics.text: Runx1_Induced
characteristics.characteristic: Submitter Id
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: An Nhe1 restriction site was inserted into the cDNA of the large Runx1 isoform to remove the first ATG and allow insertion of the tag. The Bio-V5 double tag was ligated into the Nhe1 site to create N-terminally tagged Runx1 cDNA. The tagged Runx1 cDNA was cloned into the Not1 site of a Gata1 promoter based expression vector. N-terminally tagged Runx1 cDNA was stably expressed in MEL cells containing the bacterial biotin ligase BirA. Nuclear extracts were from MEL cells were prepared as described (6). Immunoprecipitations (IP) were performed as described (Rodriguez, P et al., Isolation of transcription factor complexes by in vivo biotinylation tagging and direct binding to streptavidin beads. Methods in molecular biology 338: 305-323, Soler, E., A systems approach to analyze transcription factors in mammalian cells. Methods 53(2): 151- 162.). Bio-V5-Runx1 Immunoprecipitations (IP) from nuclear extracts were preformed using V5 affinity agarose beads from Sigma. Antibodies used in this study are listed in the supplementary data S1. Washes were preformed using HENG150 (150 mM KCl, 20 mM HEPES, 20% glycerol, 0.25 mM EDTA, 0.05% NP-40). IP�s were preformed in the presence of benzonase endonuclease to exclude the identification of complexes formed via DNA binding. Chromatin Immunoprecipitations (ChIP) were performed as described (Kolodziej, K., Optimal use of tandem biotin and V5 tags in ChIP assays. BMC molecular biology 10: 6., Soler, E., The genome592 wide dynamics of the binding of Ldb1 complexes during erythroid differentiation. Genes & development 24(3): 277-289.). Per ChIP 2x107 and per ChIP-seq 10x107 MEL cells were used.
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Runx1 chipseq in induduced MEL cells
characteristics.characteristic: title