characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-10-01T17:02:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:09:11Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2342536
characteristics.characteristic: External Id
characteristics.text: University of Pennsylvania
characteristics.characteristic: INSDC center name
characteristics.text: 2015-10-01T17:02:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:09:11Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS400221
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2266:E15.5 Ngn3^GFP/+ embryos 2
characteristics.characteristic: Submitter Id
characteristics.text: embryonic day 15.5
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002566
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: neurogenin-3-expressing endocrine progenitor cell
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Embryos were isolated from mice; the presence of vaginal plug at noon was considered as E0.5. Embryos obtained from heterozygous interbreeding were genotyped by fluorescence microscopy or were genotyped using PCR. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacture's instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008).
characteristics.characteristic: description
characteristics.text: embryo stage
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: B6.129S-Neurog3tm1(EGFP)Khk/Mmcd mouse strain
characteristics.characteristic: strain
characteristics.text: E15.5 Ngn3^GFP/+ embryos 2
characteristics.characteristic: title