characteristics.text: GSC:MIMARKS:2.1
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2011-10-08T17:00:28Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: GSC, MIxS, MIMARKS
characteristics.characteristic: ENA-KEYWORD
characteristics.text: 2018-03-09T09:52:44Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: GSC:MIENS:2.1:compliant
characteristics.characteristic: ENA-STANDARD
characteristics.text: SAMEA1706486
characteristics.characteristic: External Id
characteristics.text: MPI BREMEN
characteristics.characteristic: INSDC center name
characteristics.text: 2011-10-08T17:00:28Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-09T09:52:44Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS016143
characteristics.characteristic: SRA accession
characteristics.text: SAB2
characteristics.characteristic: Submitter Id
characteristics.text: 5
characteristics.unit: liter
characteristics.characteristic: amount or size of sample collected
characteristics.text: ENA
characteristics.characteristic: broker name
characteristics.text: 2008-09-15
characteristics.characteristic: collection date
characteristics.text: water
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_15377
characteristics.characteristic: description
characteristics.text: 17.10
characteristics.unit: percent
characteristics.characteristic: dissolved oxygen
characteristics.text: water
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_15377
characteristics.characteristic: environment
characteristics.text: 0
characteristics.characteristic: geographic location (altitude/elevation)
characteristics.text: Italy:Lago di Paola_Latium
characteristics.characteristic: geographic location (country)
characteristics.text: 0
characteristics.characteristic: geographic location (depth)
characteristics.text: 41.27, +13.038
characteristics.characteristic: geographic location (latitudeandlongitude)
characteristics.text: miens-survey
characteristics.characteristic: investigation type
characteristics.text: 96
characteristics.characteristic: library reads sequenced
characteristics.text: 192
characteristics.characteristic: library size
characteristics.text: pGEM-T Easy
characteristics.characteristic: library vector
characteristics.text: freshwater metagenome
characteristics.characteristic: organism
characteristics.text: Planctomycetes: 275276200 per liter
characteristics.characteristic: organism count
characteristics.text: initial denaturation:94degC_4min,denaturation:94degC_1min,annealing:59degC_2min,elongation:72degC_1.5min,extension:72degC_10min,33 cycles
characteristics.characteristic: pcr conditions
characteristics.text: Pla46:5'-GGA TTA GGC ATG CAA GTC-3';1392:5'-ACG GGC GGT GTG TAC-3'
characteristics.characteristic: pcr primers
characteristics.text: 37.1
characteristics.characteristic: salinity
characteristics.text: bottles
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0010132
characteristics.characteristic: sample collection device or method
characteristics.text: filtration
characteristics.characteristic: sample material processing
characteristics.text: freshwater metagenome
characteristics.characteristic: scientific_name
characteristics.text: manual
characteristics.characteristic: sequence quality check
characteristics.text: Sanger
characteristics.characteristic: sequencing method
characteristics.text: 16S rRNA
characteristics.characteristic: target gene
characteristics.text: 26.2
characteristics.unit: Celsius
characteristics.characteristic: temperature