characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-10-02T17:04:02Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:09:20Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2340759
characteristics.characteristic: External Id
characteristics.text: Instituto Gulbenkian de Ciencia
characteristics.characteristic: INSDC center name
characteristics.text: 2014-10-02T17:04:02Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:09:20Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS398430
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2270:NS5_control
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: NS5
characteristics.characteristic: cell line
characteristics.text: neuronal stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000047
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: NS5 cells were cultured as previously described (Conti et al. 2005), using laminin as a media supplement (20ug/mL), and plated at a density of 28,000/cm2 NS5 cells were infected with pBABE-IRES-GFP-puro (NS5_control) or pBABE-Ascl1-ERT2-IRES-GFP-puro (NS5_Ascl1), followed by selection in complete medium containing 1ug/mL puromycin (Calbiochem), the latter sample collected 24 hours after induction with 4-Hydroxytamoxifen at a final concentration of 50nM, while reducing EGF concentration to 5ng/mL . DNAse-seq samples from NS5 cells were prepared as described by Song et al (Genome Res, 2011). Libraries were generated as in (Boyle et al., 2008; Song and Crawford, 2010) with a slight modification made to the linkers to increase ligation efficiency (Song et al., 2011, Genome Res).
characteristics.characteristic: description
characteristics.text: pBABE-IRES-GFP-puro
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: NS5_control
characteristics.characteristic: title