characteristics.text: 2013-07-03T17:00:35Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:20:02Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2057840
characteristics.characteristic: External Id
characteristics.text: EMBL-EBI
characteristics.characteristic: INSDC center name
characteristics.text: 2013-07-03T17:00:35Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:20:02Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS223630
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1557:MDG1_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: MiliDAH and Miwi2DAH mice were crossed with Oct4/GFP trangenic mice to label germ cells with eGFP. To isolate E16.5 and postnatal day 7 germ cells, single cell suspensions of testis were obtained by two step enzymatic digestion and GFP-positive FACS-sorted. Mili and Miwi2 RNPs were immunoprecipitated as described in Reuter, M. et al. (2009). Small RNA libraries were generated with the protocol described in Hafner M. et al. (2008) but using adaptors suitable for sequencing on the Ilumina platform. Small RNA libraries were generated with the protocol described in Hafner M. et al. (2008) but using adaptors suitable for sequencing on the Ilumina platform.
characteristics.characteristic: description
characteristics.text: Mili_Ctl, Mili_C-DAH
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: testis
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: MDG1_2
characteristics.characteristic: title