characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-09-09T17:01:57Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T21:04:04Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2747197
characteristics.characteristic: External Id
characteristics.text: Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA
characteristics.characteristic: INSDC center name
characteristics.text: 2014-09-09T17:01:57Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T21:04:04Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS540005
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2885:hGO 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: human gastric organoid Protocols: hGOs were generated through a 34-day step-wise directed differentiation protocol of pluripotent stem cells. The cells were differentiated through normal embryonic stages, including endoderm formation, foregut specification and patterning, antrum specification, and growth and maturation. Total RNA was isolated from gastric organoids using the NucleoSpin RNA II kit (Macherey-Nagel 740955) RNA libraries were prepared for sequencing using standard Illumina protocols
characteristics.characteristic: description
characteristics.text: day 34
characteristics.characteristic: developmental stage
characteristics.text: 2
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: hGO 2
characteristics.characteristic: title