characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-12-01T17:02:03Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T21:50:08Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3139814
characteristics.characteristic: External Id
characteristics.text: Institute for molecular and tumor biology Philipps University Marburg Germany
characteristics.characteristic: INSDC center name
characteristics.text: 2015-12-01T17:02:03Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T21:50:08Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS611778
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3114:TA067
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: macrophage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000235
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were isolated on June 17th 2014 from healthy human donor blood of unknown gender via LSM1077 gradient centrifugation. Adherent cells were positivly selected on primaria 6 well dishes (3x10^6 monocytes per well in 2 ml PBS (PAA)) followed by 6 washing steps with PBS. Finally the monocytes were cultured for 7d in humidified incubator at 37 oC, 5 % CO2 in 2ml X-VIVO 10 (Biozym scientific, 881022), medium. Cells were treated with control (DMSO), L165 1 uM final concentration or ST247 300 nM final concentration for 1d. Cells were lysed in Peqgold (TRIzol-like solution based on phenol and chloroform). RNA was digested with DNase (MN) and purified on a Qiagen Minelute column. Illumina-compatible libraries were made using the Epicentre Scriptseq human/mouse/rat low input Gold kit.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: TA067
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005309
characteristics.characteristic: title