characteristics.text: 2011-09-16T17:00:33Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T15:29:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1094171
characteristics.characteristic: External Id
characteristics.text: CEA/Centre National de Genotypage
characteristics.characteristic: INSDC center name
characteristics.text: 2011-09-16T17:00:33Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T15:29:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS054250
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-767:46C-2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: 46C
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005484
characteristics.characteristic: cell line
characteristics.text: embryonic stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002322
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: ES cells were cultured on embryonic fibroblast feeder cells blocked with mitomycin C in D15 medium (Dulbecco Modified Eagle Medium High Glucose supplemented with 15% FBS, 2mML-glutamine, 50 units/ml penicillin, 50 ug/ml streptomycin, 0.1 mM nonessential amino acids (all from GIBCO), 0.1 mM 2-mercaptoethanol [Sigma] and 1,000 units/ml LIF). ES cells were maintained at 37C, 5% carbon dioxide, fed with fresh media daily, and transferred to new plates after trypsinization. Typically 150-200 million cells were collected for each ChIP experiment. The cellular proteins and DNA were cross-linked by the addition of formaldehyde (1% final concentration) in cell culture media. The plates were incubated for 10 min at room temperature then, the reaction was stopped by the addition of glycine (0.125 M final concentration). The cells were washed twice with 10 ml chilled PBS. Each plate was scraped with 2 ml PBS with protease inhibitors (Roche complete, 10 mM PMSF dissolved in ethanol). Untagged ES cells (46C-2), TFIIIC subunits and TCEA1 ChIPs were fragmented using MNase I. The protocol will be described in details elsewhere (M. Gerard, manuscript in preparation).
characteristics.characteristic: description
characteristics.text: transfection
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000726
characteristics.characteristic: geneticmodification
characteristics.text: untagged cell
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: 46C-2
characteristics.characteristic: title