characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-08-31T17:01:40Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:36:09Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2501486
characteristics.characteristic: External Id
characteristics.text: Pfizer Inc.
characteristics.characteristic: INSDC center name
characteristics.text: 2014-08-31T17:01:40Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:36:09Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS451356
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2510:4_L6
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: drug on Protocols: The name of drug administered is PF-04691502, the dual PI3K/mTOR inhibitor. Parental and resistant cells were plated at 150,000-200,000 cells/well in 6-well culture plates in growth medium with 10% FBS. Cells were incubated overnight and treated with DMSO (0.1% final) or PF-04691502 (1 uM final) to form three groups: parental (DMSO), drug on (drug maintained) and drug off (drug withdrawn for 48h). The TruSeq RNA sample Prep Kit (Illumina) is used to build cDNA libraries for paired-end sequencing on HiSeq. PCR is performed to amplify and enrich the ligated material to create the final cDNA Library. (Illumina TruSeq RNA Sample Preparation Guide).
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: 4_L6
characteristics.characteristic: title