characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-02-02T17:02:24Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T18:13:45Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2618370
characteristics.characteristic: External Id
characteristics.text: University of Manchester
characteristics.characteristic: INSDC center name
characteristics.text: 2015-02-02T17:02:24Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T18:13:45Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS487735
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2696:Foxc1 IIBA IP
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: The preparation of the material was as described in Kutejova et al., 2008. Second branchial arches are dissected (the whole tissue), crosslinked and frozen in order to pool enough for ChIP. Kutejova E, Engist B, Self M, Oliver G, Kirilenko P, Bobola N. Six2 functions redundantly immediately downstream of Hoxa2. Development. 2008. 135(8):1463-70. PMID: 18321982. MinElute PCR purification kit (Qiagen) ChIP-seq was performed as described in Donaldson et al., 2012, using 20 ug of Foxc1 antibody (Abcam). Donaldson I, Amin S, Hensman J, Kutejova E, Rattray M, Lawrence N, Hayes A, Ward C and Bobola N. Genome-wide occupancy links Hoxa2 to Wnt-beta-catenin signaling in mouse embryonic development. Nucleic Acids Res. 2012. 40(9):3990-4001.
characteristics.characteristic: description
characteristics.text: embryonic day 11.5
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002562
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: second branchial arch
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: mixed sex
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: Foxc1 IIBA IP
characteristics.characteristic: title