characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-10-01T17:02:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T21:02:56Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2774594
characteristics.characteristic: External Id
characteristics.text: MRC Laboratory of Molecular Biology, Cambridge
characteristics.characteristic: INSDC center name
characteristics.text: 2014-10-01T17:02:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T21:02:56Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS547350
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2939:CPSF160_GFP repl3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HeLa
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001185
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Replicate 3 Protocols: iCLIP protocol was followed to isolate RNA bound to CPSF-160 protein and amplify cDNA library as described in the manuscript. Specific barcodes were used for each RT reaction and are specified below for each sequencing run. Random barcodes were also incoperated into cDNAs to distinguish between PCR duplication of cDNA products. The final products were obtained by PCR with Illumina pair-end sequencing primers: 5-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3; 5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: CPSF160_GFP repl3
characteristics.characteristic: title