characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-12-04T17:04:46Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T18:27:39Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2593312
characteristics.characteristic: External Id
characteristics.text: CECAD, university of Cologne
characteristics.characteristic: INSDC center name
characteristics.text: 2014-12-04T17:04:46Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T18:27:39Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS479920
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2650:41946
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fission yeast
characteristics.characteristic: common name
characteristics.text: Protocols: Briefly, 500ml cultures grown at 32C were formaldehyde fixed for 30 min, harvested by centrifugation and snap frozen. Chromatin extract (CE) was prepared with a Fastprep machine (MP Biomedicals) at 6 x 20 sec, 5.5 m/s using an equal volume of 0.5 ml acid-washed glass beads (BioSpec products) to break cells. Lysates were sonicated using a Bioruptor (Diagenode) at 6 x 5, 30 s on, 30 s off, in ice cold conditions, to create CE with an average DNA size of approximately 200 bp. Immunoprecipitations (ChIps) were set up using 5 mg CE on Dynabeads Protein A with appropriate antibody (Invitrogen, LifeTechnologies). Input (CE) and ChIPs were de-crosslinked overnight, treated with DNase-free RNase (Roche) and Proteinase K (Invitrogen, LifeTechnologies), and the DNA purified using PureLink PCR micro kits (Invitrogen, LifeTechnologies). ChIP-seq libraries from the immunoprecipitated samples and corresponding input material were prepared using the NEBNext ChIP-Seq Library Prep Master Mix set for Illumina (New England Biolabs) combined with Illumina Barcodes 1-12. Briefly, and according to manufacturers instructions, DNA was end-repaired, end adaptors ligated, and the libraries amplified with 15 PCR cycles in the presence of Illumina barcodes. Libraries were purified using SPRI-beads (Agencourt, Beckman Coulter), and library size distributions and concentrations were determined by Bioanalyzer and Qubit (Invitrogen, LifeTechnologies).
characteristics.characteristic: description
characteristics.text: Pht1-myc-tagged
characteristics.characteristic: genotype
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4896
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: scientific_name
characteristics.text: Y0036-Pht1-myc-tagged
characteristics.characteristic: strain
characteristics.text: 41946
characteristics.characteristic: title