characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-03-14T17:00:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:16:47Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2401430
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF MANCHESTER
characteristics.characteristic: INSDC center name
characteristics.text: 2014-03-14T17:00:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:16:47Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS423294
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-565:FOXK2_rep2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: U2OS
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002869
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: U2OS cells, were grown in DMEM supplemented with 10% foetal bovine serum. ChIP was performed as previously described (Marais et al., 2010) using ~8-10 x 10+7 U2OS-FOXK2-HF cells stably expressing FOXK2 tagged with Flag and His tags (~4Ã-150 mm plates) or stable U2OS-HF cells containing an empty vector encoding the same tags. Marais A, Ji Z, Child ES, Krause E, Mann DJ, Sharrocks AD. 2010. Cell cycle-dependent regulation of the Forkhead transcription factor FOXK2 by CDK-cyclin complexes. J Biol Chem. 285: 35728-35739. Anti-Flag (Sigma-Aldrich, F3165)
characteristics.characteristic: description
characteristics.text: osteosarcoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000637
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: FOXK2_rep2
characteristics.characteristic: title