characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-04-04T17:00:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:09:20Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2343895
characteristics.characteristic: External Id
characteristics.text: University of Turin
characteristics.characteristic: INSDC center name
characteristics.text: 2014-04-04T17:00:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:09:20Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS401589
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2276:CONT_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: tomato
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_323
characteristics.characteristic: common name
characteristics.text: Protocols: Solanum lycopersicum cv. Moneymaker tomato plants were grown in greenhouse from March through December under natural light, which provided a photoperiod ranging from 9h to 15 h, temperature from 16 degrees C to 30 degrees C and relative humidity from 55% to 80%. The seedlings were grown in pots with sterile quartz sand (80%) and sterile pumice (20%) for non-inoculated plants or with a mixture of sand (55%), sterile pumice (20%) and Funneliformis mosseae (formerly Glomus mosseae) inoculum (25%) Gerd. and Trappe (BEG 12) purchased from Agrauxine (Bretagne, France, http://www.agrauxine.fr) for inoculated plants. Forty-five plants divided into 3 groups of fifteen plants according to their growth conditions were considered: plants grown with F. mosseae inoculum (MYC) or without inoculum were watered, once a week, with a modified Long-Ashton solution containing a low phosphorus and nitrogen concentration (3.2 uM P, 1mM N). The fertilized control plants (CONT) were grown without inoculum and watered, once a week, with a commercial fertilizer solution (7.6mM P, 13.48mM N). At the end of the experiment, the MYC plant roots were cut and fungal colonization was assessed according to the Trouvelot system using MYCOCALC software. Fruits were harvested from the CONT and MYC plants when they reached the turning stage. RNA was extracted using the pine tree-method, with the addition of 1% PVPP to the extraction buffer. For each growth condition, we used two biological replicates, each one formed by pooled RNAs of three fruits. Ten micrograms of each RNA sample was sent to Fasteris Life Science Co. (Geneva, Switzerland) where the libraries were produced.
characteristics.characteristic: description
characteristics.text: Solanum lycopersicum
characteristics.characteristic: organism
characteristics.text: Solanum lycopersicum L.
characteristics.characteristic: organism
characteristics.text: fruit
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009001
characteristics.characteristic: organism part
characteristics.text: Solanum lycopersicum
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: no Funneliformis mosseae fungus
characteristics.characteristic: stimulus
characteristics.text: CONT_1
characteristics.characteristic: title