characteristics.text: 2014-03-06T17:00:38Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:00:57Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2030635
characteristics.characteristic: External Id
characteristics.text: DUNDEE
characteristics.characteristic: INSDC center name
characteristics.text: 2014-03-06T17:00:38Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:00:57Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS179277
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1297:MietttinenSample9
characteristics.characteristic: Submitter Id
characteristics.text: 14 week
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000034
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Female mice were grown to 14 weeks of age on normal laboratory conditions before analysis Partial (70%) hepatectomy was performed for two mice of each genotype and liver was allowed to regenerate for 96 hours. Untreated mice (two of each genotype) were used as controls. Frozen liver samples (100-200 mg) were transferred to QIAzol Lysis Reagent (Qiagen) and homogenized using Precellys homogenizer (Bertin Technologies) for 30 sec. After chloroform addition, total was precipitated from the aqueous phase with 1/10 volume of 3M sodium acetate, pH 5.0 and one volume of isopropanol. 15 ug total was fragmented for 3 min at 70 degreesC in fragmentation buffer (Ambion). Fragmentation was terminated by cooling of the sample on ice and addition of EDTA to 17 mM final concentration. 2.5 ul fragmented was then mixed with 0.5 ul 100 mM MgCl2 and diluted to 30 ul with RNAse-free water.
characteristics.characteristic: description
characteristics.text: Cdk1Flox/Flox
characteristics.characteristic: genotype
characteristics.text: animal 1 Cdk1Flox/Flox prePH technical 2
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: 1
characteristics.characteristic: relative liver cell nucleus size
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: MietttinenSample9
characteristics.characteristic: title
characteristics.text: pre-partial hepatectomy
characteristics.characteristic: treatment