characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-04-03T17:00:38Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T17:07:58Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2335255
characteristics.characteristic: External Id
characteristics.text: ERASMUSMC
characteristics.characteristic: INSDC center name
characteristics.text: 2014-04-03T17:00:38Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T17:07:58Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS393449
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2225:MUTZ-3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: MUTZ-3
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005395
characteristics.characteristic: cell line
characteristics.text: myeloblast
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000835
characteristics.characteristic: cell type
characteristics.text: inv(3)(q21q26)
characteristics.characteristic: chromosomal aberration
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Cell lines were cultured using media conditions as follows. MUTZ-3: 60% alpha-MEM (Life Technologies), 20% FBS (PAA), 20% conditioned medium of cell line 5637, and 10 ng/ml hrGM-CSF (Amgen); F-36P, Kasumi-3, and UCSD-AML1: 80% RPMI 1640 (Life Technologies), 20% FBS, and 10 ng/ml GM-CSF; SB1690CB: 60% alpha-MEM, 20% FBS, 20% conditioned medium of cell line 5637; MOLM-1, and U937: 90% RPMI and 10% FBS. All cultures contained 50 U/ml penicillin and 50 μg/ml streptomycin (Sigma). Total sample RNA was extracted with phenol chloroform and reverse transcribed using Superscript II RT (Invitrogen). The cDNA was sheared with the Covaris device (Covaris S2) and further processed according to the TruSeq RNA Sample Preparation v2 Guide (Illumina).
characteristics.characteristic: description
characteristics.text: High
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/EO_0007075
characteristics.characteristic: evi1 overexpression
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: MUTZ-3
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005395
characteristics.characteristic: title