characteristics.text: 2014-06-26T17:01:24Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:35:03Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2151923
characteristics.characteristic: External Id
characteristics.text: SLU Alnarp
characteristics.characteristic: INSDC center name
characteristics.text: 2014-06-26T17:01:24Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:35:03Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS317274
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1712:Sarpo Mira sample
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: potato
characteristics.characteristic: common name
characteristics.text: Sarpo Mira
characteristics.characteristic: cultivar
characteristics.text: Protocols: Leaves from 4 week-old plants were harvested 24 hours after whole-plant infection with Phytopthora infestans RNA was isolated by RNeasy Plant Mini Kit (Qiagen) Beads with oligo(dT) were used to isolate poly(A) mRNA. Fragmentation buffer was added for interrupting mRNA to short fragments. Taking these short fragments as templates, Random hexamerprimer were used to synthesize the first-strand cDNA. The second-strand cDNA was synthesized using buffer, dNTPs, RNase H and DNA polymerase I, respectively. Short fragments were purified with QiaQuick PCR extraction kit and resolved with EB buffer for end reparation and adding poly(A). After that, the short fragments were connected with sequencing adaptors. And, for amplification with PCR, we selected suitable fragments, as templates, with respect to the result of agarose gel electrophoresis.
characteristics.characteristic: description
characteristics.text: fully expanded leaves
characteristics.characteristic: developmental stage
characteristics.text: Phytopthora infestans
characteristics.characteristic: infect
characteristics.text: Solanum tuberosum
characteristics.characteristic: organism
characteristics.text: Solanum tuberosum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4113
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organism part
characteristics.text: Solanum tuberosum
characteristics.characteristic: scientific_name
characteristics.text: frozen specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: specimen with known storage state
characteristics.text: Sarpo Mira sample
characteristics.characteristic: title