characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-08-02T17:01:19Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T19:33:01Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2697777
characteristics.characteristic: External Id
characteristics.text: Max Plank Institute for Molecular Biomedicine
characteristics.characteristic: INSDC center name
characteristics.text: 2015-08-02T17:01:19Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T19:33:01Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS525833
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-2684:control_A
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: ES-E14 cell
characteristics.characteristic: cell line
characteristics.text: embryonic stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002322
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Total RNA was extracted with GenElute total RNA miniprep kit, treated with turbo DNase and purified with RNeasy MinElute Cleanup kit. The quality was assessed on a bioanalyzer. ES cells were cultured in DMEM with GlutaMax (Life technologies) containing 15% FCS and LIF Libraries were prepared with Truseq Stranded Total RNA with Ribo-Zero Gold Prep kit (Illumina). Lipofectamine RNAi MAX (Life technologies) were used to transfect siRNA.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: 129/Ola
characteristics.characteristic: strain
characteristics.text: control_A
characteristics.characteristic: title