characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2014-01-21T17:01:07Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:41:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2171834
characteristics.characteristic: External Id
characteristics.text: University of Science of Technology of China
characteristics.characteristic: INSDC center name
characteristics.text: 2014-01-21T17:01:07Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:41:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS339479
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1629:pooled normal human testis
characteristics.characteristic: Submitter Id
characteristics.text: 28 to 35 year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: All the collections and usages of human tissues were approved by the Institutional Review Board at First Affiliated Hospital of Anhui Medical University. Testicular tissues were from three cadaver donors (aged 28 to 35 years) from the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People's Republic of China. Histological examination showed normal spermatogenesis in all of the three donators. Each patient has at least one healthy child. Total RNAs were extracted from the human testis using TRIzol (Invitrogen). These samples were then subjected to 15% (w/v) denaturing PAGE (polyacrylamide gel electrophoresis) and the small RNA fragments of 18-28 nt were isolated. The small RNA molecules were ligated to 5' and 3' adaptors sequentially and then converted to cDNA by reverse transcription followed by PCR amplifications
characteristics.characteristic: description
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: testis
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: pooled normal human testis
characteristics.characteristic: title