characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-10-16T17:48:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T16:49:13Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA2224132
characteristics.characteristic: External Id
characteristics.text: Department of Chemical Engineering, POSTECH, Pohang 790-784, South Korea
characteristics.characteristic: INSDC center name
characteristics.text: 2015-10-16T17:48:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T16:49:13Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS360481
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-1953:Pontin_KO_mESCs_OHT 5D
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Pontinf/f; CreER ES cell
characteristics.characteristic: cell line
characteristics.text: embryonic stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002322
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Used ES cells were cultured on gelatin-coated culture dishes under feeder-free conditions. ES cells were maintained in Dulbecco's modified Eagle medium (DMEM; Welgene), supplemented with 15% fetal bovine serum (FBS; Welgene), 0.055 mM beta-mercaptoethanol (GIBCO), 2 mM L-glutamine, 0.1 mM MEM nonessential amino acid, 5,000 units/ml penicillin/streptomycin, and 1,000 units/ml of LIF (Chemicon). Pontinf/f; CreER ES cells were derived from blastocysts from intercrosses between Pontinf/f; CreER and CreER transgenic mouse. CreER transgenic mice were purchased from Jackson laboratory. Oct4-depleted ES cells were generated by treating ZHBTc4 ES cells with tetracycline for 2 days. Pontin-depleted ES cells were generated by treating Pontinf/f; CreER ES cells with hydroxyl-tamoxifen (OHT) for 3 or 4 days. We obtained total RNAs from Pontinf/f; CreER ES cells at 0, 3, or 4 days post-treatment with OHT for wt ES cells, Pontin-depleted ES cells (OHT 3D) and Pontin-depleted ES cells (OHT 4D), respectively. We also isolated RNAs from ZHBTc4 ES cells at 2 days post-treatment with tetracycline for Oct4-depleted ES cells. Libraries were prepared for multiplex sequencing using Illumina True-seq RNA sample prep kit v2, according to the manufacturer's protocol.
characteristics.characteristic: description
characteristics.text: gene knock out
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000506
characteristics.characteristic: genetic modification
characteristics.text: inducible Pontin knockout
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: fresh specimen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000971
characteristics.characteristic: specimen with known storage state
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: Pontin_KO_mESCs_OHT 5D
characteristics.characteristic: title