characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2015-01-19T17:03:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-08T20:42:11Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA3109052
characteristics.characteristic: External Id
characteristics.text: The Roslin Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2015-01-19T17:03:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-08T20:42:11Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS576295
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-3048:723_4_GCCAAT_L001_BMDM
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: bone marrow macrophage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002476
characteristics.characteristic: cell type
characteristics.text: chicken
characteristics.characteristic: common name
characteristics.text: Protocols: Chicken bone marrow derived macrophage (BMDM) culture: RNA from chicken BMDM, day 7 embryos, or DF-1 cells, was extracted using TRIzol reagent, as described by the manufacturers (Invitrogen). RNA was quantified using a Nanodrop and RNA quality was assessed using Agilent nanochip Samples were prepared for mRNA sequencing using 1ug of total RNA starting material following the Illumina Truseq RNA Sequencing protocol. Resulting libraries were quality checked on an Agilent DNA 1000 bioanalyzer (Agilent Technologies, South Queensferry, UK) and then clustered onto a paired read flowcell using the Illumina v2 cluster generation kit at a 8pM concentration.
characteristics.characteristic: description
characteristics.text: Gallus gallus
characteristics.characteristic: organism
characteristics.text: Gallus gallus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9031
characteristics.characteristic: organism
characteristics.text: Gallus gallus
characteristics.characteristic: scientific_name
characteristics.text: colony-stimulating factor 1 (CSF-1)
characteristics.characteristic: stimulus
characteristics.text: 723_4_GCCAAT_L001_BMDM
characteristics.characteristic: title