characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-02-08T17:02:34Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-22T10:53:58Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104711823
characteristics.characteristic: External Id
characteristics.text: Peking University Health Science Center
characteristics.characteristic: INSDC center name
characteristics.text: 2019-02-08T17:02:34Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-22T10:53:58Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2313206
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6509:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: 9
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Norway rat
characteristics.characteristic: common name
characteristics.text: Protocols: BYD is a classic traditional Chinese medicine consists of the water extraction of astragalus root, ginseng, processed licorice, and cinnamon at a ratio of 6:2:2:1. Under the deeply anaesthetized condition after echocardiographic examinations, all rats were sacrificed for collecting the heart tissues samples. The myocardial tissues of the ischemic LV and the peri-ischemic rim (an approximately 1 mm rim of normal-appearing tissues) were removed, and the remaining tissues that consisted of non-ischemic LV were obtained. First, the widely used model produced by the occlusion of the left anterior descending coronary artery (LADCA) was employed to induce Myocardial Ischemia in rats. Prior to the operation, the rats were fasted overnight with free access to water. Briefly, an anterior thoracotomy was performed under sterile conditions to open the pericardium and the heart was then rapidly exteriorized. The LADCA was ligated approximately 2-3 mm distal from its origin with the use of a 5-0 polypropylene suture. After surgery, the rats in the sham and model group received the same volume of saline vehicle and the rats in the TCM group received a dose of BYD at 1460mg/kg for one week. The RNA integrity was analyzed on a 1% agarose gel. Beads that contained oligo (dT) were used to isolate the poly mRNA from the total RNA and then the rRNA was subsequently removed. The first strand of cDNA was synthesized by using Random Hexamers and the second strand of cDNA was synthesized by adding buffer, dNTPs, RNase H and DNA Polymerase I, then purified by QIAQuick PCR kit and eluted with EB buffer. The purified double-stranded cDNA is then subjected to end-repair, base A addition and sequencing, followed by agarose gel electrophoresis to recover the target fragment and perform PCR amplification to complete the entire library preparation.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Rattus norvegicus
characteristics.characteristic: organism
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: heart left ventricle
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002084
characteristics.characteristic: organism part
characteristics.text: Rattus norvegicus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Sprague Dawley
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001352
characteristics.characteristic: strain
characteristics.text: Sample 1
characteristics.characteristic: title