characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-05-01T17:03:45Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-28T11:31:28Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104724222
characteristics.characteristic: External Id
characteristics.text: CRUK Beatson Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2018-05-01T17:03:45Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-28T11:31:28Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2325605
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6620:Sample 4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: tumor-bearing
characteristics.characteristic: clinical information
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: KPC mice, first described by (Hingorani et al., 2005), were bred in house on a mixed background. Mice were genotyped by Transnetyx (Cordoba, TN, USA). Mice were monitored at least 3 times weekly and abdominal palpation carried out to detect pancreatic cancer. For drug treatments, adult mice of both sexes were randomly assigned to cohorts. Treatments used were: vehicle (0.1 % Tween80/0.5 % methylcellulose) p.o. twice daily, CSF-1R small molecule inhibitor (AstraZeneca, AZD7507) at 100mg/Kg p.o. twice daily; CXCR2 small molecule inhibitor (AstraZeneca, AZD5069) at 100mg/Kg p.o. twice daily, or AZD7507 and AZD5069 in combination, both at 100mg/Kg p.o. twice daily. PDAC harvested from mice into RNAl8r, then stored at -80C RNAeasy mini kit, then Turbo DNAse Illumina TruSeq Stranded mRNA Library Prep Kit
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: pancreatic ductal adenocarcinoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002517
characteristics.characteristic: disease
characteristics.text: Pdx1-Cre; LSL-KrasG12D/+; LSL-Trp53R172H/+
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: pancreas
characteristics.characteristic: organism part
characteristics.text: neoplasm
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000616
characteristics.characteristic: sampling site
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: strain
characteristics.text: Sample 4
characteristics.characteristic: title