characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-12-01T17:02:09Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-29T12:32:24Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104725948
characteristics.characteristic: External Id
characteristics.text: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College
characteristics.characteristic: INSDC center name
characteristics.text: 2018-12-01T17:02:09Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-29T12:32:24Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2327331
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6636:Sample 4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: zebrafish
characteristics.characteristic: common name
characteristics.text: Protocols: Total RNAs were extracted from liver tissues of zebrafish larvae. A total amount of 1.5μg RNA per sample was used as input material for the RNA sample preparations. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in NEB. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
characteristics.characteristic: description
characteristics.text: larval stage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000069
characteristics.characteristic: developmental stage
characteristics.text: overfeeding
characteristics.characteristic: diet
characteristics.text: Danio rerio
characteristics.characteristic: organism
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Danio rerio
characteristics.characteristic: scientific_name
characteristics.text: Sample 4
characteristics.characteristic: title