characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-12-30T04:03:40Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-13T14:19:00Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104692579
characteristics.characteristic: External Id
characteristics.text: UoH
characteristics.characteristic: INSDC center name
characteristics.text: 2019-12-30T04:03:40Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-13T14:19:00Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2293962
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6501:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: 3
characteristics.unit: month
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: intestinal stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002250
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Approximately 10,000-90,000 Lgr5-GFPHi ISCs or Lgr5-GFPLow progenitors from 3 pairs of WT or Lkb1Lgr5-KO mice 5 days after tamoxifen injection were isolated by flow cytometry. Tamoxifen induction on Lgr5GFP-Cre/+;Lkb1+/+;R26Td/+ mouse leads to the activation of TdTomato lineage tracing while Lkb1 remains wildtype (Lkb1-WT) in intestinal Lgr5+ stem cells and their progeny in comparison to tamoxifen induction on Lgr5GFP-Cre/+;Lkb1fl/fl;R26Td/+ mouse leads to the activation of TdTomato lineage tracing and inactivation of Lkb1 (Lkb1-KO) in intestinal Lgr5+ stem cells and their progeny. Total RNA was extracted using TRI Reagent (MRC, TR118) Ovation Universal RNA-Seq System kit was used for lllumina library preparations (NuGEN Technologies., CA, USA). Purified total RNAs were used for ribosomal removal as outlined in the kit manual. Libraries were purified with AMPure XP beads (Beckman Coulter Inc., MA, USA).
characteristics.characteristic: description
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: Lgr5GFP-Cre/+;Lkb1+/+;R26Td/+
characteristics.characteristic: genotype
characteristics.text: high_2138_wt
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: small intestine
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002108
characteristics.characteristic: organism part
characteristics.text: Lkb1 wildtype; Lgr5-GFP high
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: tamoxifen; i.p 0.15 milligram per gram body weight
characteristics.characteristic: stimulus
characteristics.text: C57BL/6 x CD1
characteristics.characteristic: strain
characteristics.text: Sample 1
characteristics.characteristic: title