characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-03-23T17:02:19Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-13T14:31:16Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104692600
characteristics.characteristic: External Id
characteristics.text: University of Strasbourg
characteristics.characteristic: INSDC center name
characteristics.text: 2018-03-23T17:02:19Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-13T14:31:16Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2293983
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6563:VRIS_lps3h37_2
characteristics.characteristic: Submitter Id
characteristics.text: 45
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: mononuclear cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000842
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation from blood samples of VPER and VRIS using Ficoll-Paque™. PBMC cells (106 cells) were seeded in 24-well plates in RPMI1640 supplemented with fetal calf serum (FCS), penicillin, streptomycin, amphotericin B (all from Invitrogen) and stimulated with LPS (1 µg/mL; from Salmonella Abortus equi ; Sigma, Saint-Quentin-Fallavier, France) for 3 h or 6 h at 37 °C in a humidified atmosphere containing 5% CO2. The control condition without LPS stimulation is named mi. Description of the sample naming: lps: stimualtion with lps - mi: no stimulation - 3h/6h: lps stimulation for 3 or 6 hours - _1 or _2: replicate 1 or 2 - VPER: subject VPER - VRIS: subject VRIS Trizol SOLiD Total RNA-Seq Kit
characteristics.characteristic: description
characteristics.text: hyper IgD syndrome
characteristics.ontologyTerms: http://www.orpha.net/ORDO/Orphanet_343
characteristics.characteristic: disease
characteristics.text: 2
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: hyper IgD syndrome symptoms
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: VRIS_lps3h37_2
characteristics.characteristic: title