characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-03-30T17:01:44Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-20T11:44:47Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104707995
characteristics.characteristic: External Id
characteristics.text: Institute of Healthy Ageing,
characteristics.characteristic: INSDC center name
characteristics.text: 2018-03-30T17:01:44Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-20T11:44:47Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2309378
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6584:Sample 52
characteristics.characteristic: Submitter Id
characteristics.text: 10
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: Dissection into RNAlater solution Dissected 6h into the light cycle into ice-cold RNAlater. The base (1SY) medium contained 100 g/l autolysed yeast (MP Biomedicals, OH, USA), 50 g/l sucrose (Tate & Lyle, London, UK), 15 g/l agar (Sigma-Aldrich, Dorset, UK), 30 ml/l nipagin (Chemlink Specialities, Dorset, UK), and 3 ml/l propionic acid (Sigma-Aldrich, Dorset, UK). The EAA-replete control medium comprised DR food with an EAA supplement dissolved in pH 4.5 water (final concentrations in fly media: L-arginine 0.43 g/l, L-histidine 0.21 g/l, L-isoleucine 0.34 g/l, L-leucine 0.48 g/l, L-lysine 0.52 g/l, L-methionine 0.1 g/l, L-phenylalanine 0.26 g/l, L-threonine 0.37 g/l, L-tryptophan 0.09 g/l, L-valine 0.4 g/l: all suppled by Sigma). EAA+rapamycin food consisted of EAA-replete medium with a rapamycin supplement (LC laboratories, MA, USA) dissolved in ethanol, to a final concentration of 200 µM in the diet. Outbred wild-type Dahomey flies bearing the endosymbiont Wolbachia were cultured on a 12:12 light cycle at 25oC and 60% humidity, on 1SY medium. All flies were maintained at large population sizes to maintain outbred genetic diversity. Eggs were collected following an 18h lay on grape juice agar, added to bottles of 1SY at a standardised density and cultured to adulthood. Newly emerged experimental females were mated on fresh food for 48h, lightly CO2 anaesthetised, then allocated to experimental diets without males (10 females/vial). Qiagen total RNA isolation kit Illumina TruSeq Stranded mRNA Sample Prep kit
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7227
characteristics.characteristic: organism
characteristics.text: arthropod fat body
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0003917
characteristics.characteristic: organism part
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Dahomey, Wolbachia +ve
characteristics.characteristic: strain
characteristics.text: Sample 52
characteristics.characteristic: title