characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-04-07T17:01:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-28T19:12:00Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104725442
characteristics.characteristic: External Id
characteristics.text: 1st Center for Biotechnology, Bielefeld University, Universitatsstraße 27, 33615 Bielefeld, Germany 2nd Institute for Biology-Microbiology, Freie Universitat Berlin, Konigin-Luise-Str. 12-16, 14195 Berlin, Germany
characteristics.characteristic: INSDC center name
characteristics.text: 2018-04-07T17:01:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-28T19:12:00Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2326825
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6633:Sd_DEL_dod.2_exp_PL12
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: S. davawensis and S. coelicolor strains were grown in YS in the presence or absence of plumbagin (15 µM). Cells were harvested by centrifugation in the exponential growth phase (after 18 h of growth) and from the stationary growth phase (after 48 h of growth) and frozen in liquid nitrogen. 30 ml YS in 200 ml baffled Erlenmeyer flasks was inoculated with 2 x 10^6 spores. The cultures were incubated at 30°C and 180 rpm.. none or plumbagin (15 µM) Total RNA was isolated from three biological replicates using in part a previously reported protocol Ortiz de Orue Lucana and Schrempf (2000) and the RNeasy Mini Kit along with a DNase Kit (both from Qiagen). Briefly, mycelia were washed with 25 mM Tris, pH 7.5. 1 g mycelia was resuspended in a 3 ml-solution of 4 M guanidinthiocyanate, 25 mM sodium citrate, pH 7, 0.5 % laurylsarcosine and 0.1 M 2-mercaptoethanol, and treated with 0.1 mm silica beads by vortexing for 3 min. The silica beads and the cell debris were removed by centrifugation. 500 µl of the supernatant were used for isolation according to the protocols described in the RNeasy Mini and DNase Kit. The RNA was controlled was tested for possible DNA contaminations by PCR. RNA concentrations and quality were determined using Trinean Xpose (Gentbrugge, Belgium) and Agilent RNA Nano 6000 kit on Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). Ribo-Zero rRNA Removal Kit (Bacteria) from Illumina (San Diego, CA, USA) was used to deplete ribosomal RNA. The quality of rRNA-depleted RNA samples was checked on Agilent 2100 Bioanalyzer again. Illumina TruSeq® Stranded mRNA low sample protocol starting with rRNA-depleted total RNA
characteristics.characteristic: description
characteristics.text: deletion mutant dod.2 (BN159_1333)
characteristics.characteristic: genotype
characteristics.text: exponential phase
characteristics.characteristic: growth condition
characteristics.text: Streptomyces davaonensis JCM 4913
characteristics.characteristic: organism
characteristics.text: Streptomyces davaonensis JCM 4913
characteristics.characteristic: organism
characteristics.text: Streptomyces davaonensis JCM 4913
characteristics.characteristic: scientific_name
characteristics.text: Sd_DEL_dod.2_exp_PL12
characteristics.characteristic: title