characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-12-31T17:02:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-23T09:50:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104717295
characteristics.characteristic: External Id
characteristics.text: Warwick Medical School, University of Warwick, UK
characteristics.characteristic: INSDC center name
characteristics.text: 2018-12-31T17:02:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-23T09:50:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2318678
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6607:Sample 7
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: macrophage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000235
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Blood samples were collected from 3 healthy volunteers and peripheral blood mononuclear cells (PBMCs) isolated by density gradient centrifugation on Ficoll. PBMCs were differentiated into macrophages using macrophage colony-stimulating factor (M-CSF). Cells were stimulated for 3h as detailed and total RNA extracted. PBMCs were differentiated to macrophages in RPMI + 10% FCS with 50ng/ml M-CSF for 8 days before stimulation. PBMC-derived macrophages were stimulated with either media alone, 200ng/ml lipid A or 50ng/ml TNF alpha for 3h before RNA isolation. ~2x10^6 cells were stimulated per condition. RNA was extracted using the mirVana RNA isolation kit according to the manufacturer's instructions. Input RNA quality was assessed on a TapeStation2200 using the RNA Screen Tape to confirm high RNA quality (RIN score > 8) according to manufacturers instructions. Input RNA quantity was assessed using a Qubit 2.0 Fluorometer and the Qubit RNA BR Assay Kit according to manufacturers instructions. Library was constructed using the Illumina TruSeq stranded total RNA kit according to manufacturers instructions with an input of 500 ng total RNA / sample. This kit includes ribosomal RNA removal by Ribozero treatment. The quality of final libraries was checked on a TapeStation2200 using the D1000 Screen Tape.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: 3
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: peripheral blood mononuclear cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_2000001
characteristics.characteristic: progenitor cell type
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 7
characteristics.characteristic: title