characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-05-01T17:03:45Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-29T15:30:32Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104726211
characteristics.characteristic: External Id
characteristics.text: Department of Biochemistry University of Geneva
characteristics.characteristic: INSDC center name
characteristics.text: 2018-05-01T17:03:45Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-29T15:30:32Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2327594
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6623:Untreated 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HeLa-MZ
characteristics.characteristic: cell line
characteristics.text: epithelial cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000066
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: HeLa-MZ cells (DOI: 10.1038/nature03866) a sub-clone isolated from the ATCC HeLa parental line was selected for high-content imaging. HeLa-MZ cells were treated in triplicate with control-conditioned, or Wnt3a-conditionedmedia for 2h or 6h before cells were collected and RNA isolated. Then (time = 0), the control samples”, grown in non-conditioned media- were collected and the RNA extracted. The other samples were treated with either L cell-conditioned media or L Wnt3a-cell conditioned media for a further 2h or 6h before the RNA was collected. The conditioned media was prepared from L Cells (ATCC: CRL-2648; control media) and L Wnt3a Cells (ATCC: CRL-2647; Wnt3a media) (as described here: Cytoskeletal reorganization by soluble Wnt-3a protein signalling. Genes Cells. 1998 Oct;3(10):659-70.) Purification of total RNA was done with the RNeasy Mini Kit from Qiagen according to the manufacturer’s instructions. The concentration, purity, and integrity of the RNA were measured with the Picodrop Microliter UV-Vis Spectrophotometer (Picodrop), and the Agilent 2100 Bioanalyzer (Agilent Technologies), together with the RNA 6000 Series II Nano Kit (Agilent) according to the manufacturer’s instructions. TruSeq mRNA Stranded mRNA Library kit from Illumina (#20020595) was used for the library preparation with 1000 ng of total RNA as input.
characteristics.characteristic: description
characteristics.text: cervical adenocarcinoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001416
characteristics.characteristic: disease
characteristics.text: non-conditioned media
characteristics.characteristic: growth condition
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: uterine cervix
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000002
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Untreated 1
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001461
characteristics.characteristic: title