characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-01-01T17:01:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-09T16:53:47Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104678762
characteristics.characteristic: External Id
characteristics.text: Karolinska Institutet, Department of Women's and Children's Health, Center for Molecular Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2019-01-01T17:01:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-09T16:53:47Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2280145
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6592:Sample 22
characteristics.characteristic: Submitter Id
characteristics.text: 10
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Embryonic and fetal bladders and genital tissue were obtained by surgeons from terminated pregnancies after informed consent and with ethics approval. Samples were taken from weeks 7 (n=2), 7,5 (n=1), 8 (n=3), 9 (n=4) for the bladder tissues and from weeks 7 (n=3), 8 (n=3), 9 (n=3) as well as 10 (n=4) for the genital tissues, respectively. Fresh tissues were dissected and immediately submerged in 5 volumes of RNAlater Stabilization Solution (Cat No. AM7020, Thermoisher Scientific, Waltham, MA), incubated at 4°C overnight and then transferred to -20°C. RNA was extracted by using TissueLyser LT (Cat No. 85600, QIAGEN, Hilden, Germany) and RNeasy Fibrous Tissue Mini Kit (cat. no. 74704, QIAGEN, Hilden, Germany). The RNA was measured both quantitatively and qualitatively using NanoDrop (Thermofisher Scientific, Waltham, MA) and Bioanalyzer 2100 (Agilent, Santa Clara, CA) instruments. Paired-end libraries of 2x126 bp with poly-A selection according to Illumina TruSeq Stranded mRNA protocol.
characteristics.characteristic: description
characteristics.text: late embryo
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: reproductive organ
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0003133
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 22
characteristics.characteristic: title