characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-08-31T17:03:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-23T15:03:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104717432
characteristics.characteristic: External Id
characteristics.text: The Children’s Hospital of Philadelphia
characteristics.characteristic: INSDC center name
characteristics.text: 2018-08-31T17:03:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-23T15:03:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2318815
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6617:Sample 6
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: CD4-positive helper T cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000492
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: The following cells were sorted from human children healthy donor tonsils: naive CD4+CD45RO- T cells (Naive) and CD4+CD45RO+CD25-CXCR5hiPD1hi follicular helper CD4+ T cells (TFH). For each of the TFH and Naive libraries, 50,000 live cells were harvested. The ATAC-seq libraries were generated following a previously published protocol (Buenrostro et al., Nature Methods, 2013). The nuclei were isolated from the 50,000 cells. The isolated nuclei were treated with Tn5 Transposes from Nextera DNA Sample Preparation Kit and the transposed DNA was purified using a Qiagen MinElute Kit. The transposed DNA libraries were amplified by PCR for 12 cycles and isolated by Ampure XP beads.
characteristics.characteristic: description
characteristics.text: juvenile stage
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: TC60
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002865
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: tonsil
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002372
characteristics.characteristic: organism part
characteristics.text: CD4+CD45RO
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 6
characteristics.characteristic: title