characteristics.text: PowerWater_DNA_isolation_kit
characteristics.characteristic: DNA extraction method
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-05-28T04:03:41Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-30T14:27:45Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1045418
characteristics.characteristic: External Id
characteristics.text: HELLENIC CENTRE FOR MARINE RESEARCH
characteristics.characteristic: INSDC center name
characteristics.text: 2019-05-28T04:03:41Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-30T14:27:45Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2351768
characteristics.characteristic: SRA accession
characteristics.text: HX_m_7
characteristics.characteristic: Submitter Id
characteristics.text: I._Santi
characteristics.characteristic: collected by
characteristics.text: 1_l
characteristics.characteristic: collected_volume
characteristics.text: 2014-09-27T09:15:00Z
characteristics.characteristic: collection date
characteristics.text: Eastern_Mediterranean_coastal_seawater;_different_nutrient_amount_manipulation;_Control_(no_addition,N:P_3.2);_Low_(N:P_4.7);_High_(N:P_6.5);_triplicate_mesocosms;_three_experimental_days_were_sampled;_16S_&_18S_amplicons;_illumina_MiSeq_sequence_platform
characteristics.characteristic: description
characteristics.text: Yes
characteristics.characteristic: environmental sample
characteristics.text: 0
characteristics.characteristic: experimental day
characteristics.text: high
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/EO_0007075
characteristics.characteristic: experimental treatment
characteristics.text: Mediterranean Sea
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: lat=35.33_lon=25.28
characteristics.characteristic: lat lon
characteristics.text: 1_m
characteristics.characteristic: mesocosm_sampling_depth
characteristics.text: marine metagenome
characteristics.characteristic: organism
characteristics.text: 5'CCTACGGGNGGCWGCAG
characteristics.characteristic: primer_f
characteristics.text: Klindworth_A,_Pruesse_E,_Schweer_T,_Peplies_J,_Quast_C,_Horn_M,_et_al._(2012)._Evaluation_of_general_16S_ribosomal_RNA_gene_PCR_primers_for_classical_and_next-generation_sequencing-based_diversity_studies._Nucleic_Acids_Res._e-pub_ahead_of_print,_doi:_10.1093/nar/gks808.
characteristics.characteristic: primer_f_reference
characteristics.text: 5'GACTACNVGGGTATCTAATCC
characteristics.characteristic: primer_r
characteristics.text: Apprill_A,_Mcnally_S,_Parsons_R,_Weber_L._(2015)._Minor_revision_to_V4_region_SSU_rRNA_806R_gene_primer_greatly_increases_detection_of_SAR11_bacterioplankton._Aquat_Microb_Ecol_75:_129�137.
characteristics.characteristic: primer_r_reference
characteristics.text: b
characteristics.characteristic: replicate
characteristics.text: marine metagenome
characteristics.characteristic: scientific_name
characteristics.text: 1_m
characteristics.characteristic: seawater_depth_field
characteristics.text: -80
characteristics.characteristic: storage temperature
characteristics.text: 16S
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005724
characteristics.characteristic: target gene
characteristics.text: Nutrient_addition_mesocosm_experiment
characteristics.characteristic: title