characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-10-16T17:02:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-20T16:28:12Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104708351
characteristics.characteristic: External Id
characteristics.text: Department of Microbiology & Immunology, University of Texas Medical Branch
characteristics.characteristic: INSDC center name
characteristics.text: 2018-10-16T17:02:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-20T16:28:12Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2309734
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6614:Sample 11
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Yellow Fever infectious clones (IC) were generated by site-directed mutagenesis (SDM) of either pANCR 17D (IC) or pANCR Asibi IC as a template. QuickChange XLII SDM kit (Strategene) along with mutagenic primer sets were used to generate infectious clones as per the manufacturer’s protocol. Stock cDNA YF IC were amplified in E. coli (MC1061) and plasmid harvested by large-scale extraction. Full-length YF RNA was generated by in vitro transcription of purified cDNA using SP6-Scribe standard RNA in vitro transctiption kit (Cellscript) according to the manufacturer’s protocol; the reaction was incubated at 37oC for 2.5 hours. The entire reaction was electroporated into 6.7x106 monkey kidney Vero cells. Cell supernatant was collected when infected cells showed 80% cytopathic effect (CPE). RNA from the YF ICs were extracted using QIAamp Viral RNA mini kit (Qiagen), following the manufacturer’s guidelines. NGS libraries were constructed using TruSeq RNA v2 kit as recommended by the manufacturer.
characteristics.characteristic: description
characteristics.text: NS2B-L109I substitution
characteristics.characteristic: genotype
characteristics.text: grown in monkey kidney Vero cells
characteristics.characteristic: growth condition
characteristics.text: Yellow fever virus
characteristics.characteristic: organism
characteristics.text: Yellow fever virus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_11090
characteristics.characteristic: organism
characteristics.text: Yellow fever virus
characteristics.characteristic: scientific_name
characteristics.text: 17D-204
characteristics.characteristic: strain
characteristics.text: Sample 11
characteristics.characteristic: title