characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-03-22T17:02:19Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-12T17:32:12Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104679931
characteristics.characteristic: External Id
characteristics.text: University of Cambridge Wellcome Sanger Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2018-03-22T17:02:19Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-12T17:32:12Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2281314
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6578:HGPS_mutant_Repeat2
characteristics.characteristic: Submitter Id
characteristics.text: 10
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mice were euthanized by CO2 inhalation followed by cervical dislocation. Upon dissection the mouse tissues were snap-frozen in liquid N2 and immediately stored at -80ºC. RNA was extracted from tissues from n>5 independent mice/group using the RNeasy fibrous tissue mini kit (50) (cat. No.7404; Qiagen) and quantified using the NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific). 2μg RNA/sample was used to produce cDNA using the High-Capacity RNA-to-cDNA kit (cat. No. 4387406; Applied Biosystems/Thermo Fisher Scientific). qPCR was carried out using the TaqMan system (Universal Master Mix II, with UNG, 4440038; Applied Biosystems/Thermo Fisher Scientific) on an Applied Biosystems Quant Studio 3 machine. n≥3 mice were used for each genotype, with 50ng cDNA for each sample run in triplicate or quadruplicate. Thermo Fisher Scientific Nat10 (Mm00462302_m1) and Cdkn1a/p21 (Mm00432448_m1) primers were used as experimental probes while Gapdh (Mm99999915_g1), Actb (Mm00607939_s1) and/or Rn18s (Mm03928990_g1) were used as endogenous controls. The Relative Standard Curve pre-set programme was used throughout and data analysis was performed using the Relative Quantification application, powered by the Thermo Fisher Scientific cloud platform. Illumina: TruSeq Stranded mRNA Library Prep Kit (Cat. No. RS-122-2101)
characteristics.characteristic: description
characteristics.text: Hutchinson-Gilford progeria syndrome
characteristics.characteristic: disease
characteristics.text: HGPS-/-
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: heart
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000948
characteristics.characteristic: organism part
characteristics.text: HGPS knockout
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: HGPS_mutant_Repeat2
characteristics.characteristic: title