characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-03-01T17:02:13Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-12T16:18:31Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104679923
characteristics.characteristic: External Id
characteristics.text: University of Halle Julius Bernstein Institute of Physiology
characteristics.characteristic: INSDC center name
characteristics.text: 2019-03-01T17:02:13Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-12T16:18:31Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2281306
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6600:AR_150626_AR_6
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: LLC-WRC 256
characteristics.characteristic: cell line
characteristics.text: epithelial cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000066
characteristics.characteristic: cell type
characteristics.text: Norway rat
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were washed with PBS before lysing with Trizol. Both cell lines were cultured in RPMI medium supplemented with 10% fetal calf serum (FCS) and for Walker-256 cells additionally with 10 mM L-glutamine. Cells were kept at 37°C in a humidified 5% CO2 atmosphere and were sub-cultivated twice per week. For the experiments cells were kept in FCS-lacking medium for 24 h at pH 6.6 or pH 7.4. The control pH of 7.4 was obtained by buffering the medium with NaHCO3 and 20 mM HEPES pH adjustment with 1M NaOH. Extracellular acidosis (pH 6.6) was applied using bicarbonate + 20 mM MES (morpholinoethanesulfonic acid)-buffered medium, pH adjustment to 6.6 with 1M NaOH. Trizol according to the manufacturer's protocol Illumina TruSeq Small RNA Library according to the manufacturer's protocol
characteristics.characteristic: description
characteristics.text: mammary gland adenocarcinoma
characteristics.ontologyTerms: http://identifiers.org/medgen/C4288013
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Rattus norvegicus
characteristics.characteristic: organism
characteristics.text: Rattus norvegicus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10116
characteristics.characteristic: organism
characteristics.text: mammary gland
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0000817
characteristics.characteristic: organism part
characteristics.text: Rattus norvegicus
characteristics.characteristic: scientific_name
characteristics.text: Walker
characteristics.characteristic: strain
characteristics.text: AR_150626_AR_6
characteristics.characteristic: title