characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-04-03T17:03:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-28T10:33:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104724183
characteristics.characteristic: External Id
characteristics.text: UCSF School of Medicine Department of Neurological Surgery
characteristics.characteristic: INSDC center name
characteristics.text: 2018-04-03T17:03:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-28T10:33:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2325566
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6270:Sample 28
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: mononuclear cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000842
characteristics.characteristic: cell type
characteristics.text: peptide vaccine immunotherapy
characteristics.characteristic: clinical information
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Samples were collected in accordance with our approved IRB (University of Pittsburgh Medical Center). PBMCs were isolated from whole blood using FicollTM, according to the manufacturers protocol, at the Immune Monitoring and Cell Production Laboratory (IMCPL) at the Hillman Cancer Center (Pittsburgh, PA). PBMCs were collected from LGG patients at multiple time relative to the initial peptide vaccine treatment including, 0 weeks (w; pretreatment), 6w, 16w, and when available 34w, 70w, and 86w time points. Dnase-1-treated RNA was isolated using a Direct-zol RNA isolation kit (Zymo). RNA quality and concentration was determined by RNA Screen Tape (Agilent) using a 4200 TapeStation (Agilent). All RNA integrity numbers (RIN) exceeded RIN-8. mRNA libraries were prepared using TruSeq Stranded mRNA (PolyA+) Library Prep Kit High Throughput (Illumina)
characteristics.characteristic: description
characteristics.text: juvenile stage
characteristics.characteristic: developmental stage
characteristics.text: Low Grade Glioma
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/MONDO_0021637
characteristics.characteristic: disease
characteristics.text: P6
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Sample 28
characteristics.characteristic: title