characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-01-03T17:03:19Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-04-04T15:15:40Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA1054242
characteristics.characteristic: External Id
characteristics.text: Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, SE-41296, Sweden
characteristics.characteristic: INSDC center name
characteristics.text: 2019-01-03T17:03:19Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-04-04T15:15:40Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2359843
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6650:Eth_Rep1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: baker's yeast
characteristics.characteristic: common name
characteristics.text: Protocols: 10OD of cells were collected, pelleted and snap frozen in liquid nitrogen. The S. cerevisiae strain CEN.PK113-7D was pre-cultured in a batch culture in Delft media with 2% glucose at 30°C and 200 rpm in shake flasks and then transferred to the chemostats in duplicates. Four different media compositions with a limitation in a different nutrient were employed in the chemostat runs. The temperature was also set to 30°C. The dilution rate was fixed to 0.1 and the cells were grown for 4 days to achieve stable cell numbers in the culture. RNA was extracted using the RNeasy® Mini Kit from QIAGEN. Cells were mechanically disrupted using a FastPrep®-24 from MPbio in combination with the lysing matrix tubes type C from MPbio. The FastPrep was run 3 times for 20 seconds with 4.0m/s settings and a 5 minute break in between each run. RNA quality was assessed using NanoDrop and Bioanalyzer. The non-amplification non-tagging CAGE protocol for Illumina sequencing (nAnT-iCAGE) as previously published by Murata et. al. 2014 was used on two biological replicates of each condition, starting with 5 micrograms of extracted RNA.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Delft media with 2 percent glucose
characteristics.characteristic: growth condition
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4932
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: scientific_name
characteristics.text: CEN.PK113-7D
characteristics.characteristic: strain
characteristics.text: Eth_Rep1
characteristics.characteristic: title