characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-03-05T04:06:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-20T13:18:08Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104708061
characteristics.characteristic: External Id
characteristics.text: University of Heidelberg, Germany
characteristics.characteristic: INSDC center name
characteristics.text: 2020-03-05T04:06:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-20T13:18:08Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2309444
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6606:Anto7
characteristics.characteristic: Submitter Id
characteristics.text: 30
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: C57Bl/6 Ly5.1 male mice lethally irradiated have been transplanted at 8 weeks of age with bone marrow from Hbb(th3/+)/Tfr2(+/+) or Hbb(th3/+)/Tfr2(-/-) mice (Bone marrow in a mixed genetic background C57BL6/129Sv). After transplantation, the mice have been maintained in a standard diet for 22 weeks and euthanised for organs extraction and analysis. Mice were euthanized 22 weeks after BM transplant and the spleen was extracted and SNAP-frozen. total RNA has been extracted with Trizol mRNA-Seq libraries were created according to the Truseq stranded mRNA Illumina protocol.
characteristics.characteristic: description
characteristics.text: Beta-thalassemia
characteristics.characteristic: disease
characteristics.text: C57Bl/6 Ly5.1 BM Hbb(Th3/+)
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: spleen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002106
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: transplanted with bone marrow from Tfr2 wild type mouse
characteristics.characteristic: stimulus
characteristics.text: C57BL/6
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004472
characteristics.characteristic: strain
characteristics.text: Anto7
characteristics.characteristic: title