characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-04-01T17:02:03Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-22T14:01:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104712312
characteristics.characteristic: External Id
characteristics.text: Vanderbilt University
characteristics.characteristic: INSDC center name
characteristics.text: 2018-04-01T17:02:03Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-22T14:01:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2313695
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6615:P1 mutant beta cell - Biological replicate 2
characteristics.characteristic: Submitter Id
characteristics.text: 1
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: beta cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000168
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: MytDelpanc and MipeGFP islets were collected from mice postnatal day 1. The islets from 2-3 mice of the same genotype were pooled for each sample. Islets were dispersed using trypsin and FACS purified based on eGFP expression. Propidium Iodine was used to select live cells. MytDelpanc genotype: Myt1tm1.1Ggu/tm1.1Ggu;Myt1Ltm1.1Ggu/tm1.1Ggu;St18tm1.1Ggu/tm1.1Ggu; Tg(Pdx1-cre)89.1Dam/+; Tg(Ins1-EGFP)1Hara/J. Myt1tm1.1Ggu/tm1.1Ggu information: http://www.informatics.jax.org/allele/genoview/MGI:3765058?counter=1 and Tg(Pdx1-cre)89.1Dam/+ allele information: http://www.informatics.jax.org/allele/MGI:2684317. The Myt1Ltm1.1Ggu allele has two loxP sites flanking the exons #12 and #13. Cre-mediated inactivation produces an allele that makes no Myt1L protein. The St18tm1.1Ggu allele has two loxP sites flanking the exons #9 and #10. Cre-mediated inactivation produces an allele that makes no St18 protein. Mice used in this experiment contained pancreatic cell specific inactivation of a family of genes, Myt1, Myt1L, and St18. These mutants carried MipeGFP transgene for beta-cell labeling. Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacture's instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008).
characteristics.characteristic: description
characteristics.text: pancreatic specific knockout of Myt1, Myt1L, and St18
characteristics.characteristic: disease
characteristics.text: Myt1tm1.1Ggu/tm1.1Ggu;Myt1Ltm1.1Ggu/tm1.1Ggu;St18tm1.1Ggu/tm1.1Ggu; Tg(Pdx1-cre)89.1Dam/+; Tg(Ins1-EGFP)1Hara/J
characteristics.characteristic: genotype
characteristics.text: mixed pool of 2-3 mice
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: islet of Langerhans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000006
characteristics.characteristic: organism part
characteristics.text: pancreatic specific knockout of Myt1, Myt1L, and St18
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: P1 mutant beta cell - Biological replicate 2
characteristics.characteristic: title