characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-02-01T17:01:51Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-26T14:34:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104718326
characteristics.characteristic: External Id
characteristics.text: Postdoctoral Fellow
characteristics.characteristic: INSDC center name
characteristics.text: 2019-02-01T17:01:51Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-26T14:34:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2319709
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6626:Sample 20
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: H9
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0003045
characteristics.characteristic: cell line
characteristics.text: induced pluripotent stem cell
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Pluripotent cell lines were obtained as follows: H9 hESC were purchased from WiCell (WA09) and the hiPSC line MRS2-6 was derived in house from fibroblasts taken from a patient with Mitchell-Riley syndrome, using the CytoTune-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific, #A16517) in accordance with the manufacturer’s instructions. Human pluripotent stem cells were maintained in mTeSR1 media on Matrigel-coated tissue culture plastic, and differentiated into pancreatic progenitors using the STEMdiff Pancreatic Progenitor kit (STEMCELL Technologies, 05120). Differentiation was carried out according to the manufacturer’s instructions with the following modifications: (1) cells were initially seeded into 12 well plates (Corning, 353043) at a density of 10e6 cells/well, and (2) stage 1 was extended to last 3 days - rather than 2 - by repeating the final day’s treatment. Samples were harvested for RNA isolation on differentiation days 4, 7, 8 and 12. All tissue culture was carried out in a 5% CO2 air environment at 37oC. RNA was isolated from samples harvested from differentiation cultures using an RNeasy mini kit (Qiagen, cat # 74104). All RNA samples had an RNA integrity number > 9. RNA-seq libraries were generated using the NEBNext Ultra RNA Library Prep Kit, (NEB, E7530L). Messenger RNA was enriched by hybridization to oligo-dT.
characteristics.characteristic: description
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: differentiation into pancreatic progenitor cells
characteristics.characteristic: growth condition
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 20
characteristics.characteristic: title