characteristics.text: ERC000014
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2021-03-01T12:35:29Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-24T03:42:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104717589
characteristics.characteristic: External Id
characteristics.text: MPIEVB
characteristics.characteristic: INSDC center name
characteristics.text: 2021-03-01T12:35:29Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-24T03:42:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2318972
characteristics.characteristic: SRA accession
characteristics.text: AU110404_1_stool_S70
characteristics.characteristic: Submitter Id
characteristics.text: 127
characteristics.characteristic: ap in u/l
characteristics.text: 0.6
characteristics.characteristic: basophile in %
characteristics.text: 14
characteristics.characteristic: bsg1h
characteristics.text: 2.43
characteristics.characteristic: calzium in mmol/l
characteristics.text: 2014-02-14
characteristics.characteristic: collection date
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: 3.7
characteristics.characteristic: crp in mg/l
characteristics.text: Chronic recurrent multifocal osteomyelitis (CRMO) is a rare autoinflammatory bone disease for which a lack of bacterial involvement is a key diagnostic feature to distinguish it from other symptomatically related diseases. However, the growing evidence suggesting an involvement of the host-associated microbiota in rheumatic disorders together with the now wide accessibility of modern metagenomic methods warrant a closer examination of CRMO. In this study, we show through bacterial 16S rRNA gene profiling that numerous features of the oral- and fecal microbial communities differentiate children with and without CRMO. Notably, communities in diseased children are characterized by a lack of potential probiotic bacteria in the fecal community and an overabundance of known pathobionts in the oral microbial communities. Of special interest is the HACEK group, a set of commonly known oral pathogens that are implicated in the development of several acute and chronic diseases such as osteitis and rheumatoid arthritis. Furthermore, we observe that gut bacterial communities appear to reflect an altered host physiology more strongly than the oral community in the diseased children, which could suggest an oral origin followed by propagation and/or responses beyond the oral cavity. Bacterial communities may therefore have direct- and/or indirect effects on the emergence and development of a pediatric disease formally considered to be non-bacterial.
characteristics.characteristic: description
characteristics.text: no
characteristics.characteristic: drug usage
characteristics.text: area of developed space with high usage intensity
characteristics.characteristic: environment (biome)
characteristics.text: area of developed space with high usage intensity
characteristics.characteristic: environment (feature)
characteristics.text: feces
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCIT_C13234
characteristics.characteristic: environment (material)
characteristics.text: 5.8
characteristics.characteristic: eosinophile in %
characteristics.text: 4.74
characteristics.characteristic: erythrocytes /pl
characteristics.text: Germany
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 54.330333
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 10.140611
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: 13
characteristics.characteristic: hb in g/dl
characteristics.text: 38
characteristics.characteristic: hkt in %
characteristics.text: 10
characteristics.unit: year
characteristics.characteristic: host age
characteristics.text: feces
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCIT_C13234
characteristics.characteristic: host body product
characteristics.text: colon
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001155
characteristics.characteristic: host body site
characteristics.text: chronic recurrent multifocal osteomyelitis
characteristics.characteristic: host disease status
characteristics.text: chronic recurrent multifocal osteomyelitis
characteristics.characteristic: host phenotype
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: host sex
characteristics.text: AU110404
characteristics.characteristic: host subject id
characteristics.text: human-associated
characteristics.characteristic: human associated environmental package
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: 210
characteristics.characteristic: ldh in u/l
characteristics.text: 34.1
characteristics.characteristic: lymphos in %
characteristics.text: 27.4
characteristics.characteristic: mch in pg
characteristics.text: 34.6
characteristics.characteristic: mchc in g/dl
characteristics.text: 79
characteristics.characteristic: mcv in fl
characteristics.text: 10.7
characteristics.characteristic: monozyten in %
characteristics.text: 48.8
characteristics.characteristic: neutrophile in %
characteristics.text: PMID: 27053239
characteristics.characteristic: nucleic acid amplification
characteristics.text: PSP Spin Stool DNA Kit
characteristics.characteristic: nucleic acid extraction
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: PMID: 27053239
characteristics.characteristic: pcr conditions
characteristics.text: 27F,338R
characteristics.characteristic: pcr primers
characteristics.text: CRMO
characteristics.characteristic: project name
characteristics.text: collection in Sarstedt feces collection tube
characteristics.characteristic: sample collection device or method
characteristics.text: freezing
characteristics.characteristic: sample material processing
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Illumina
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000724
characteristics.characteristic: sequencing method
characteristics.text: 16S rRNA
characteristics.characteristic: target gene
characteristics.text: V1V2
characteristics.characteristic: target subfragment
characteristics.text: 327
characteristics.characteristic: thrombocytes/nl
characteristics.text: Analysis of the fecal and oral microbiota in chronic recurrent multifocal osteomyelitis
characteristics.characteristic: title