characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-04-03T17:03:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-03-23T20:24:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104717579
characteristics.characteristic: External Id
characteristics.text: The Children’s Hospital of Philadelphia
characteristics.characteristic: INSDC center name
characteristics.text: 2018-04-03T17:03:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-03-23T20:24:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2318962
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6621:Sample 3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: T follicular helper cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002038
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Data were derived from healthy donor children's tonsils.The following cells were sorted from human tonsils: naive CD4+CD45RO- T cells (Naive) and CD4+CD45RO+CD25-CXCR5hiPD1hi follicular helper CD4+ T cells (TFH). For each of the TFH and Naive libraries, 10 million cells were harvested and fixed. The initial 3C libraries were generated following a previously published protocol (Hughes et al., Nature genetics, 2014). The DNA was digested using DpnII, then re-ligated together using T4 DNA ligase, and finally isolated by phenol/chloroform extraction. The 3C libraries were sonicated to an average size of 300bp. Agilent SureSelect library capture protocol was then applied with capture probes targeting both ends of DpnII restriction fragments that overlap promoters of protein-coding, noncoding, antisense, snRNA, miRNA, snoRNA and lincRNA transcripts, totaling 36,691 RNA baited fragments. The bait list is provided in the file baits_1frag.bed.
characteristics.characteristic: description
characteristics.text: juvenile stage
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: 31
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: tonsil
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002372
characteristics.characteristic: organism part
characteristics.text: CD4+CD45RO+CD25-CXCR5hiPD1hi
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 3
characteristics.characteristic: title