characteristics.text: E9.5
characteristics.characteristic: ArrayExpress-DevelopmentalStage
characteristics.text: whole embryo
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000922
characteristics.characteristic: ArrayExpress-OrganismPart
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: ArrayExpress-Sex
characteristics.text: Mus Musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: ArrayExpress-Species
characteristics.text: C57BL/6N
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004472
characteristics.characteristic: ArrayExpress-StrainOrLine
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2018-06-13T17:03:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2018-06-14T11:24:44Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104328822
characteristics.characteristic: External Id
characteristics.text: SC
characteristics.characteristic: INSDC center name
characteristics.text: 2018-06-13T17:03:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2018-06-14T11:24:44Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS1953800
characteristics.characteristic: SRA accession
characteristics.text: 26cc3f5c-8e54-11e7-84c2-3c4a9275d6c8
characteristics.characteristic: Submitter Id
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Total RNA from a single heterozygous female E9.5 27 somite mouse embryo, with heterozygous Smg9 / C57BL/6N parents. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. Embryo was originally genotyped by detecting disrupted allele with qPCR on yolk sac DNA as wildtype. However, lacZ expression detected in the sequence suggests heterozygous, therefore the genotype has been altered accordingly. Using the yolk sac the embryo was originally sexed as male, however, analysis of Xist and Y chromosome genes in the sequence data suggests female and the gender has been altered accordingly.
characteristics.characteristic: sample description
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: C57BL/6N
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004472
characteristics.characteristic: strain
characteristics.text: M02318283_Smg9_het6
characteristics.characteristic: title