characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-03-09T04:03:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-16T16:15:25Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6087374
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF MANCHESTER
characteristics.characteristic: INSDC center name
characteristics.text: 2020-03-09T04:03:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-16T16:15:25Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3887781
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8446:siKLF5 rep2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: OE19
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002308
characteristics.characteristic: cell line
characteristics.text: epithelial cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000066
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were cultured in 37deg incubator with 5% CO2. Cells were treated with 100pmol siRNA using RNAiMAX for 72 hours. RNA was collected using Qiagen RNeasy kit. Cells were lysed directly in the plate. RNA-seq libraries were constructed using Illumina TruSeq Stranded mRNA kit.
characteristics.characteristic: description
characteristics.text: esophageal adenocarcinoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000478
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: esophagus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001043
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: siKLF5 rep2
characteristics.characteristic: title