characteristics.text: F-CCTACGGGAGGCAGCAG;R-GGACTACHVGGGTWTCTAAT
characteristics.characteristic: 16s_primer
characteristics.text: ERC000014
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-10-31T04:04:09Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-16T20:46:07Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6087612
characteristics.characteristic: External Id
characteristics.text: MPIEVB
characteristics.characteristic: INSDC center name
characteristics.text: 2019-10-31T04:04:09Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-16T20:46:07Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3888018
characteristics.characteristic: SRA accession
characteristics.text: G02138-L1
characteristics.characteristic: Submitter Id
characteristics.text: 1 polyp
characteristics.unit: L
characteristics.characteristic: amount or size of sample collected
characteristics.text: V3V4-one step
characteristics.characteristic: amplification protocol
characteristics.text: 2016-09-29
characteristics.characteristic: collection date
characteristics.text: starlet sea anemone
characteristics.characteristic: common name
characteristics.text: investigation of the whole adult polyp
characteristics.characteristic: description
characteristics.text: aquatic
characteristics.characteristic: environment
characteristics.text: saline water aquarium
characteristics.characteristic: environment (biome)
characteristics.text: artificial sea water
characteristics.characteristic: environment (feature)
characteristics.text: artificial sea water
characteristics.characteristic: environment (material)
characteristics.text: Germany
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 54.331849
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 10.144127
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: Schleswig-Holstein, Kiel, Zoological Institute
characteristics.characteristic: geographic location (region and locality)
characteristics.text: whole animal
characteristics.characteristic: host body habitat
characteristics.text: whole animal
characteristics.characteristic: host body product
characteristics.text: whole animal
characteristics.characteristic: host body site
characteristics.text: Nvectensis_1
characteristics.characteristic: host subject id
characteristics.text: host-associated
characteristics.characteristic: host-associated environmental package
characteristics.text: human-associated
characteristics.characteristic: human associated environmental package
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: PCR
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000415
characteristics.characteristic: library construction method
characteristics.text: i5-TAGCGAGT;i7-GACATAGT
characteristics.characteristic: multiplex identifiers
characteristics.text: DOI:10.1186/s40168-019-0743-1
characteristics.characteristic: nucleic acid extraction
characteristics.text: Nematostella vectensis
characteristics.characteristic: organism
characteristics.text: 1
characteristics.characteristic: organism count
characteristics.text: initial denaturation:98degC_30s;30times;denaturation:98degC_9s;annealing:55degC_60s;extension:72degC_90s;final extension:72degC_10min;storage:10degC_infinity
characteristics.characteristic: pcr conditions
characteristics.text: F-AATGATACGGCGACCACCGAGATCTACACTAGCGAGTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTTCCCTACGGGAGGCAGCAG;R-CAAGCAGAAGACGGCATACGAGATGACATAGTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTATCGGACTACHVGGGTWTCTAAT
characteristics.characteristic: pcr primers
characteristics.text: Comparative analysis of amplicon and metagenomic sequencing methods reveals key features in the evolution of animal metaorganisms
characteristics.characteristic: project name
characteristics.text: DOI:10.1186/s40168-019-0743-1
characteristics.characteristic: sample collection device or method
characteristics.text: DOI:10.1186/s40168-019-0743-1
characteristics.characteristic: sample material processing
characteristics.text: Nematostella vectensis
characteristics.characteristic: scientific_name
characteristics.text: Illumina
characteristics.characteristic: sequencing method
characteristics.text: 16S rRNA
characteristics.characteristic: target gene
characteristics.text: V3V4
characteristics.characteristic: target subfragment
characteristics.text: Nematostella vectensis associated microbial sample
characteristics.characteristic: title