characteristics.text: F-AGRGTTYGATYMTGGCTCAG;R-GCTGCCTCCCGTAGGAGT
characteristics.characteristic: 16s_primer
characteristics.text: ERC000014
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-10-31T04:04:09Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-16T20:43:46Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6087560
characteristics.characteristic: External Id
characteristics.text: MPIEVB
characteristics.characteristic: INSDC center name
characteristics.text: 2019-10-31T04:04:09Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-16T20:43:46Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3887966
characteristics.characteristic: SRA accession
characteristics.text: G05162-L1
characteristics.characteristic: Submitter Id
characteristics.text: 5 individuals (females)
characteristics.unit: L
characteristics.characteristic: amount or size of sample collected
characteristics.text: V1V2-two step
characteristics.characteristic: amplification protocol
characteristics.text: 2016-09-20
characteristics.characteristic: collection date
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Drosophila melanogaster gut tissue associated microbial communities
characteristics.characteristic: description
characteristics.text: terrestrial
characteristics.characteristic: environment
characteristics.text: laboratory environment�
characteristics.characteristic: environment (biome)
characteristics.text: culture vial
characteristics.characteristic: environment (feature)
characteristics.text: gel
characteristics.characteristic: environment (material)
characteristics.text: Germany
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 54.331849
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 10.144127
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: Schleswig-Holstein, Kiel, Zoological Institute
characteristics.characteristic: geographic location (region and locality)
characteristics.text: Gastrointestinal tract
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/HP_0011024
characteristics.characteristic: host body habitat
characteristics.text: Gastrointestinal tract
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/HP_0011024
characteristics.characteristic: host body product
characteristics.text: Gastrointestinal tract
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/HP_0011024
characteristics.characteristic: host body site
characteristics.text: Dmelanogastergut_2
characteristics.characteristic: host subject id
characteristics.text: host-associated
characteristics.characteristic: host-associated environmental package
characteristics.text: human-associated
characteristics.characteristic: human associated environmental package
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: PCR
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000415
characteristics.characteristic: library construction method
characteristics.text: i5-AAGGAGTA;i7-TCCTCTAC
characteristics.characteristic: multiplex identifiers
characteristics.text: DOI:10.1186/s40168-019-0743-1
characteristics.characteristic: nucleic acid extraction
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: 5
characteristics.characteristic: organism count
characteristics.text: 1. step:initial denaturation:98degC_30s;25times;denaturation:98degC_10s;annealing:55degC_30s;extension:72degC_60s;final extension:72degC_10min;storage:10degC_infinity; 2. step:initial denaturation:98degC_30s;10times;denaturation:98degC_9s;annealing:55degCs;extension:72degC_60s;final extension:72degC_10min;storage:10degC_infinity???
characteristics.characteristic: pcr conditions
characteristics.text: step 1 F-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTCAGRGTTYGATYMTGGCTCAG;step 1 R-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCAGCTGCCTCCCGTAGGAGT;step 2 F-AATGATACGGCGACCACCGAGATCTACACAAGGAGTATCGTCGGCAGCGTC;step 2 R-CAAGCAGAAGACGGCATACGAGATTCCTCTACGTCTCGTGGGCTCGG
characteristics.characteristic: pcr primers
characteristics.text: Comparative analysis of amplicon and metagenomic sequencing methods reveals key features in the evolution of animal metaorganisms
characteristics.characteristic: project name
characteristics.text: DOI:10.1128/aem.01903-13
characteristics.characteristic: sample collection device or method
characteristics.text: DOI:10.1128/aem.01903-13
characteristics.characteristic: sample material processing
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: Illumina
characteristics.characteristic: sequencing method
characteristics.text: 16S rRNA
characteristics.characteristic: target gene
characteristics.text: V1V2
characteristics.characteristic: target subfragment
characteristics.text: Drosophila melanogaster associated microbial sample
characteristics.characteristic: title