characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-01-30T04:05:10Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-14T16:35:00Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6082041
characteristics.characteristic: External Id
characteristics.text: Division of Obstetrics and Gynecology Department of Clinical Science, Intervention and Technology Karolinska Institutet, Stockholm, Sweden
characteristics.characteristic: INSDC center name
characteristics.text: 2020-01-30T04:05:10Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-14T16:35:00Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3882455
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8381:Run1_C-sec
characteristics.characteristic: Submitter Id
characteristics.text: 28 and 32 and 36
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Whole ovaries were received, medulla was trimmed away using scalpels and remaining cortex was cry-preserved in 1x1x1mm pieces using slow-freezing in Ethylene Glycol. After thawing of tissue, pieces were cut into smaller pieces using scalpels and enzymatically digested (Liberase, Collagenase IV, DNase I) on 37C. After digestion into single cells, cells were either prepared for Dead Cell Removal kit (run1) or FACS (run2). Single droplets containing one cell/droplet was prepared from the cell suspension in 0.04% BSA using 10x chromium controller. Nucleic acid extraction was performed per in chip inside 10x chromium controller. 10x Genomics single cell 3' protocol was followed.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: pool of donors
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: ovary
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000992
characteristics.characteristic: organism part
characteristics.text: ovarian cortex
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0013191
characteristics.characteristic: sampling site
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Run1_C-sec
characteristics.characteristic: title