characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2021-06-01T00:11:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2021-06-01T00:11:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA5883804
characteristics.characteristic: External Id
characteristics.text: Departament of Biochemistry and Molecular Biology Universidade Federal do Parana (UFPR)
characteristics.characteristic: INSDC center name
characteristics.text: 2021-06-01T00:11:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2021-06-01T00:11:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3672260
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8268:A01 +NO3 02
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Cells were collected and stored in RNAlater stabilization solution (Thermo Fisher Scientific). Cells were cultivated in NFbHP-malate supplemented with 1 mM NH4Cl, at 30C and 250 rpm. When cells reached OD600 = 0.4, cells were collected and stored in RNAlater. Additionally, the remaining cells were incubated for a further 30 minutes after addition of 10 mM NH4Cl (Ammonium shock) or 10 mM KNO3 (Nitrate shock). After incubation, cells were collected and stored in RNAlater. RNA was extracted using Trizol (Thermo Fisher Scientific), and contaminant DNA was removed using TURBO DNase (Thermo Fisher Scientific). rRNA was removed using Ribo-Zero rRNA Removal kit (Gram-negative bacteria) (Illumina). cDNA libraries were constructed using Ion Total RNASeq kit v2 (Thermo Fisher Scientific).
characteristics.characteristic: description
characteristics.text: A01 mutant
characteristics.characteristic: genotype
characteristics.text: nitrate shock
characteristics.characteristic: growth condition
characteristics.text: Herbaspirillum seropedicae
characteristics.characteristic: organism
characteristics.text: Herbaspirillum seropedicae
characteristics.characteristic: organism
characteristics.text: Herbaspirillum seropedicae
characteristics.characteristic: scientific_name
characteristics.text: SmR1
characteristics.characteristic: strain
characteristics.text: A01 +NO3 02
characteristics.characteristic: title