characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-10-02T04:04:59Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-14T11:44:58Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6080664
characteristics.characteristic: External Id
characteristics.text: EMBL
characteristics.characteristic: INSDC center name
characteristics.text: 2020-10-02T04:04:59Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-14T11:44:58Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3881765
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8453:rna2FPCp15r1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: induced oligodendrocyte
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Pericytes (PCs) were cultured in pericyte medium. Mouse embryonic fibroblasts (MEFs) were cultured in MEF medium. Oligodendrocytes (pOPCs and iOPCs) were cultured in OPC medium. The cells were cultured in a humidified incubator at 37C under 5% CO2. To induce differentiation of MEFs or PCs into oligodendrocytes, PC and MEFs were transduced with virues containing Olig2/Sox10 (2F-pOPCs) or Olig2/Sox10/Nkx6.2 (3F-pOPCs). RNAs were extracted from 1 Mio. cells using RNeasy Kit (Qiagen), followed by DNase digestion using TURBO DNase (Thermo Fisher). RNA was quantified using Nanodrop and RIN score quality assessment was determine using Bioanalyzer. PolyA-mRNA was isolated from 1 microg of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) before sequencing library preparation. mRNAs were isolated from 1 microg of total RNA using a PolyA selection kit (NEB) and sequencing libraries were prepared following instructions from NEBs Ultra Library Preparation Kit for Illumina. All samples were barcoded, amplified in 12x PCR cycles, pooled and sequenced on a HiSeq2500 Sequencer (Illumina) using a 50 bp single-end run.
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: rna2FPCp15r1
characteristics.characteristic: title