characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-01-13T04:04:19Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-17T12:32:41Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6096701
characteristics.characteristic: External Id
characteristics.text: Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland
characteristics.characteristic: INSDC center name
characteristics.text: 2020-01-13T04:04:19Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-17T12:32:41Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3897095
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8445:Sample 2
characteristics.characteristic: Submitter Id
characteristics.text: 6 to 8
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Inguinal lymph nodes were harvested, collected into RPMI 1640 medium containing 2% FCS, 20mM Hepes (all from Lonza), 0.375 mg/ml Collagenase P (Sigma Aldrich) and 100 μg/ml DNase1 (Applichem) and shred into small pieces using 22 guage needles. Samples were incubated at 37C for 60 min, with resuspension and collection of the supernatant every 15 min into PBS containing 0.5% FCS and 10mM EDTA. Stromal cells were enriched by depleting CD45+ hematopoietic cells and Ter119+ erythrocytes using MACS microbeads (Miltenyi). VSV immunization protocol: Mice were immunized sub-cutaneously (s.c) with 2x10E7 PFU UV-inactivated vesicular stomatitis virus (VSV) on day 0 and boosted with a subsequent s.c. immunization with 5x10E7 PFU UV-inactivated VSV on day 4. UV-inactivation was performed by exposing virus to UV light for 20 minutes. Reticular cells / germinal center B cells from draining inguinal lymph nodes were examined at day 12 following the first VSV immunization. EYFP+ TdTomato+ cells from lymph nodes of naïve Cxcl13-Cre/TdTomato EYFP mice and immunized Cxcl13-Cre/TdTomato EYFP or Cxcl13-Cre/TdTomato Cxcl12fl/fl EYFP mice were sorted using the BioRad S3 Cell Sorter or the BD FACS Melody cell sorter. Single cell suspensions were run on the 10x Chromium (10x Genomics) system. The cDNA library generation was performed following the established commercial protocol for Chromium Single Cell 3’ Reagent Kit (v2 Chemistry)
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: developmental stage
characteristics.text: Tg(Cxcl13-Cre/TdTomato)+ x R26-EYFP++
characteristics.characteristic: genotype
characteristics.text: 8 mice pooled
characteristics.characteristic: individual
characteristics.text: dendritic cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000451
characteristics.characteristic: inferred cell type
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: inguinal lymph node
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001542
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: C57BL/6N
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004472
characteristics.characteristic: strain
characteristics.text: Sample 2
characteristics.characteristic: title