characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-10-31T04:02:53Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-21T13:29:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6103914
characteristics.characteristic: External Id
characteristics.text: Faculty of Biology, Ludwig Maximilians University Munich
characteristics.characteristic: INSDC center name
characteristics.text: 2019-10-31T04:02:53Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-21T13:29:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3904596
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6800:Dppa3KO_B8_r1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: embryonic stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002322
characteristics.characteristic: cell type
characteristics.text: B8
characteristics.characteristic: clone
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were harvested by trypsinization ESCs were cultured in N2B27 +2i/LIF Column purification For RRBS, genomic DNA was isolated using the QIAamp DNA Mini Kit (QIAGEN), after an overnight lysis and proteinase K treatment. RRBS library preparation was performed as described previously (Boyle et al. 2012; doi.org/10.1186/gb-2012-13-10-r92), with the following differences: bisulfite treatment was performed using the EZ DNA Methylation-Gold™ Kit (Zymo Research Corporation) according to the manufacturer's protocol.
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: DPPA3 KO
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: inner cell mass
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000087
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Dppa3KO_B8_r1
characteristics.characteristic: title