characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-12-25T04:02:59Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-15T12:03:09Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6086173
characteristics.characteristic: External Id
characteristics.text: UCSF
characteristics.characteristic: INSDC center name
characteristics.text: 2019-12-25T04:02:59Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-15T12:03:09Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3886581
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8435:Ing-CD81-Pos-R3
characteristics.characteristic: Submitter Id
characteristics.text: 10
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: adipose precursor cell
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Lin- Sca1+ CD81+ and Lin- Sca1+ and CD81- cells were sorted from scWAT, vWAT and BAT of wildtype C57BL6 mice using FACS. Total RNA was isolated from sorted CD81+ and CD81- cells from wild type C57BL/6 mice at 10-weeks-old using RNeasy Micro Kit (QIAGEN). cDNA synthesis and amplification was performed from 50 ng of total RNA using SMARTer® Universal Low Input RNA Kit (Clontech) according to manufacturer’s instruction.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: subcutaneous adipose tissue
characteristics.characteristic: organism part
characteristics.text: CD81 positive
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: Ing-CD81-Pos-R3
characteristics.characteristic: title