characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-12-20T17:03:59Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-08T09:11:28Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6058639
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF MANCHESTER
characteristics.characteristic: INSDC center name
characteristics.text: 2019-12-20T17:03:59Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-08T09:11:28Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3859769
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8411:Sample 5
characteristics.characteristic: Submitter Id
characteristics.text: 10 to 14
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: macrophage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000235
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mice. Colonies of LysM-Bmal1-/- and CX3CR1-Bmal1-/- mice were maintained in the University of Manchester Biological Services Facility (BSF). Where WT only mice were used C57BL/6 were purchased from Envigo. All genetically modified mice were created on a C57BL/6 background and housed in 12:12 light/dark (L:D) cycles with food and water supplied ad libitum. All protocols were approved by the University of Manchester Animal Welfare and Ethical Review Body and the Animals (Scientific Procedures) Act 1986 UK Home Office guidelines were strictly adhered to. Conditional targeted cre positive mice, were sex and age matched with floxed/floxed littermate controls; PECs for ex vivo were harvested from male mice. Genotyping was performed on all experimental animals. Primary Cell Culture. PECS from LysM-Bmal1-/- and littermate controls (n=5) were collected from mice by peritoneal lavage. PECS were isolated resuspended in complete RPMI and seeded at 5x105 cells per well in a 24 well plate. After washing peritoneal macrophages were lysed and RNA extracted with ReliaPrep RNA cell miniprep (Promega). RNA was extracted with ReliaPrep RNA cell miniprep (Promega). Truseq stranded mRNA Assay. Paired end.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Bmal1 flox/flox
characteristics.characteristic: genotype
characteristics.text: 5
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: peritoneal cavity
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001179
characteristics.characteristic: organism part
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: Sample 5
characteristics.characteristic: title