characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-03-03T04:03:03Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-15T18:42:37Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6086377
characteristics.characteristic: External Id
characteristics.text: Garvan Institute of Medical Research
characteristics.characteristic: INSDC center name
characteristics.text: 2020-03-03T04:03:03Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-15T18:42:37Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3886785
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8436:Lx9_DNA_2
characteristics.characteristic: Submitter Id
characteristics.text: 8 to 10
characteristics.unit: month
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Samples were prepared from mouse pancreas. Total RNA was isolated using TRIzol (Invitrogen) according to the manufacturer’s instructions. For quantitative real-time PCR (qRT-PCR), 200 ng RNA was treated with DNase I (Qiagen) for 30 min at 37°C, before cDNA was synthesized using the Superscript III first-strand synthesis system with oligo(dT) primers for coding and random primers for non-coding transcripts. RNA integrity was tested before and after depletion of ribosomal RNA (rRNA) using the Bioanalyzer2100 (Agilent, Santa Clara, CA). Following rRNA depletion, samples were incubated with DNase I (Qiagen) at 37°C for 30 minutes according to the manufacturer’s instructions. Indexed sequencing libraries were constructed using an Agilent Stranded RNA-seq kit (Agilent, Santa Clara, CA).
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: developmental stage
characteristics.text: lx9c11-/-
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: pancreas
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001264
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: Lx9_DNA_2
characteristics.characteristic: title