characteristics.text: ERC000014
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-04-14T16:27:51Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2020-03-03T16:23:04Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA5554310
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF ZURICH, Institute of Molecular Life Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2020-04-14T16:27:51Z
characteristics.characteristic: INSDC first public
characteristics.text: 2020-03-03T16:23:04Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3356352
characteristics.characteristic: SRA accession
characteristics.text: 604
characteristics.characteristic: Submitter Id
characteristics.text: 2016-01-29
characteristics.characteristic: collection date
characteristics.text: DNA from lung sputum was extracted using the High Pure PCR Template Preparation Kit (Roche, Basel, Switzerland) and subjected to methylated DNA depletion using the NEBNext Microbiome Enrichment kit (New England Biolabs Inc, Massachusetts, USA). Next-generation sequencing libraries were prepared using the TruSeq DNA Nano Library Prep kit (Illumina Inc, California, USA). Libraries were sequenced using the Illumina HiSeq 4000 platform (Illumina Inc, California, USA) in paired-end mode (2 X 125 bp). Reads were mapped to the human reference genome (assembly hg38) using BowTie2. Reads concordantly mapping to the human genome were discarded from further analysis.
characteristics.characteristic: description
characteristics.text: lung
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002048
characteristics.characteristic: environment (biome)
characteristics.text: lung
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002048
characteristics.characteristic: environment (feature)
characteristics.text: sputum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCIT_C13278
characteristics.characteristic: environment (material)
characteristics.text: Switzerland
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 47°25'51.2N
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 9°23'08.7E
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: sputum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCIT_C13278
characteristics.characteristic: host body product
characteristics.text: 11
characteristics.characteristic: host subject id
characteristics.text: human-associated
characteristics.characteristic: human associated environmental package
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: cystic fibrosis
characteristics.ontologyTerms: http://www.orpha.net/ORDO/Orphanet_586
characteristics.characteristic: lung/pulmonary disorder
characteristics.text: lung metagenome
characteristics.characteristic: organism
characteristics.text: Strain-resolved Dynamics of the Lung Microbiome in Patients with Cystic Fibrosis
characteristics.characteristic: project name
characteristics.text: lung metagenome
characteristics.characteristic: scientific_name
characteristics.text: Illumina HiSeq 4000
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0008563
characteristics.characteristic: sequencing method
characteristics.text: non-human DNA from lung sputum of a cystic fibrosis patient
characteristics.characteristic: title