characteristics.text: ERC000022
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-10-01T08:10:22Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2020-01-31T08:34:13Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA5704827
characteristics.characteristic: External Id
characteristics.text: UPPSALA UNIVERISTY
characteristics.characteristic: INSDC center name
characteristics.text: 2020-10-01T08:10:22Z
characteristics.characteristic: INSDC first public
characteristics.text: 2020-01-31T08:34:13Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3508482
characteristics.characteristic: SRA accession
characteristics.text: Jaedraas-E
characteristics.characteristic: Submitter Id
characteristics.text: 0.015
characteristics.unit: L
characteristics.characteristic: amount or size of sample collected
characteristics.text: 2013-10
characteristics.characteristic: collection date
characteristics.text: Several samples were collected for DNA extraction and amplification of approximately 1500 bp of the rRNA gene. A total of 8 samples successfully amplified and the PCR products of these were pooled to generate the amplicon library for sequencing.
characteristics.characteristic: composite design/sieving (if any)
characteristics.text: Amplicon library from podzol soil, mineral elluvial soil layer (E). Approximately 1500bp of the rRNA was amplified using primers ITS1F and LR5, and sequenced on a PacBio RS II system.
characteristics.characteristic: description
characteristics.text: temperate evergreen needleleaf forest (ENVO_01000383)
characteristics.characteristic: environment (biome)
characteristics.text: soil
characteristics.characteristic: environment (feature)
characteristics.text: podzol (ENVO_00002257)
characteristics.characteristic: environment (material)
characteristics.text: Sweden
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 0.05-0.08
characteristics.unit: m
characteristics.characteristic: geographic location (depth)
characteristics.text: 185
characteristics.unit: m
characteristics.characteristic: geographic location (elevation)
characteristics.text: 60.82
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 16.5
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: Gastrikland:Jadraas
characteristics.characteristic: geographic location (region and locality)
characteristics.text: E horizon
characteristics.characteristic: horizon
characteristics.text: manual inspection
characteristics.characteristic: horizon method
characteristics.text: mimarks-survey
characteristics.characteristic: investigation type
characteristics.text: soil metagenome
characteristics.characteristic: organism
characteristics.text: initial denaturation:95degC_10min; denaturation:95degC_0.75min; annealing=58degC_0.75min; elongation:72degC_1.5min; final elongation:72degC_10min; total cycles:30
characteristics.characteristic: pcr conditions
characteristics.text: FWD:CTTGGTCATTTAGAGGAAGTAA REV:TCCTGAGGGAAACTTCG
characteristics.characteristic: pcr primers
characteristics.text: Environmental long read amplicons of soil fungal across soil horizons
characteristics.characteristic: project name
characteristics.text: soil was kept on ice then frozen when back to lab
characteristics.characteristic: sample material processing
characteristics.text: 0.5
characteristics.unit: g
characteristics.characteristic: sample weight for DNA extraction
characteristics.text: soil metagenome
characteristics.characteristic: scientific_name
characteristics.text: PacBio
characteristics.characteristic: sequencing method
characteristics.text: soil
characteristics.characteristic: soil environmental package
characteristics.text: frozen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000922
characteristics.characteristic: storage conditions (fresh/frozen/other)
characteristics.text: rRNA
characteristics.characteristic: target gene
characteristics.text: ITS and LSU
characteristics.characteristic: target subfragment
characteristics.text: Amplicon library from podzol soil, mineral elluvial soil layer (E).
characteristics.characteristic: title