characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-12-31T11:11:40Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-17T11:47:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6095671
characteristics.characteristic: External Id
characteristics.text: Dpt. Gastroenterology and Hepatology University Hospitals Leuven
characteristics.characteristic: INSDC center name
characteristics.text: 2020-12-31T11:11:40Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-17T11:47:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3896066
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8449:Sample 97
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Endoscopy-derived inflamed colonic biopsies were obtained cross-sectionally from IBD patients (n = 70 Crohn’s disease [CD], n = 60 ulcerative colitis [UC]) with endoscopically proven active disease. Biopsies were taken at the most affected site (expect for the healthy individuals), at the edge of the ulcer, and stored in RNALater buffer (Ambion, Austin, TX, USA) with subsequent preservation at -80°C. Total RNA from the colonic biopsies was extracted using the AllPrep DNA/RNA Mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions, after tissue lysis using the FastPrep Lysing Matrix D tubes (MP Biomedicals, Brussels, Belgium) with RLT lysis buffer (Qiagen, Hilden, Germany). RNA quantity and integrity was assessed with a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, Waltham, MA, USA) and a 2100 Bioanalyzer (Agilent, Waldbronn, Germany). Extracted RNA was stored at -80°C until further processing. Library preparation using the TruSeq Stranded mRNA protocol (Illumina, San Diego, USA) according to the manufacturer’s instructions
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: developmental stage
characteristics.text: ulcerative colitis
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000729
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: colon
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001155
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 97
characteristics.characteristic: title