characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-06-30T04:03:54Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-07T09:29:45Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6056370
characteristics.characteristic: External Id
characteristics.text: CGR, Institute of Integrative Bioloy, The University of Liverpool, UK
characteristics.characteristic: INSDC center name
characteristics.text: 2020-06-30T04:03:54Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-07T09:29:45Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3857503
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8409:Sample 3
characteristics.characteristic: Submitter Id
characteristics.text: 20
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: horse
characteristics.characteristic: common name
characteristics.text: Protocols: Synovial fluid derived from post mortem horses colelcted at an abattoir. Synovial fluid was from the metacarophalangeal joint. At collection synovial fluid was immediately placed on ice and then centrifuged for 10 min at 3000 g 4oC. The supernatant removed and stored at -80oC. Synovial fluid was treated to reduce viscosity with 1µg/ml of hyaluronidase at 37˚C for 1 hr, centrifuged at 1,000g for 5 min, and supernatant used for total RNA extraction using miRNEasy serum kits (Qiagen, Crawley, UK). Trizol followed by miRNAEasy Serum kit (Qiagen) Small RNA Library Prep Set for Illumina (New England Biosciences (NEB), Ipswich, USA) but with the addition of a Cap-Clip™ Acid Pyrophosphatase (Cell script, Madison, USA) step to remove any 5’ cap structures and size selected using a range 120-300bp
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: developmental stage
characteristics.text: osteoarthritis
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002506
characteristics.characteristic: disease
characteristics.text: mild
characteristics.characteristic: disease staging
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: 5
characteristics.characteristic: individual
characteristics.text: Equus caballus
characteristics.characteristic: organism
characteristics.text: Equus caballus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9796
characteristics.characteristic: organism
characteristics.text: synovial fluid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001090
characteristics.characteristic: organism part
characteristics.text: Equus caballus
characteristics.characteristic: scientific_name
characteristics.text: male neutered
characteristics.characteristic: sex
characteristics.text: Sample 3
characteristics.characteristic: title