characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-10-02T04:04:59Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-14T10:50:08Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6080614
characteristics.characteristic: External Id
characteristics.text: EMBL
characteristics.characteristic: INSDC center name
characteristics.text: 2020-10-02T04:04:59Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-14T10:50:08Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3881715
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8452:2FPCp25r2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: induced oligodendrocyte
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Pericytes (PCs) were cultured in pericyte medium. Mouse embryonic fibroblasts (MEFs) were cultured in MEF medium. Oligodendrocytes (pOPCs and iOPCs) were cultured in OPC medium. The cells were cultured in a humidified incubator at 37C under 5% CO2. To induce differentiation of MEFs or PCs into oligodendrocytes, PC and MEFs were transduced with virues containing Olig2/Sox10 (2F-pOPCs) or Olig2/Sox10/Nkx6.2 (3F-pOPCs). 20.000 cells were harvested and washed once in cold PBS for 5 min at 500 x g at 4 C. The pellet was gently resuspended in 50ul of cold lysis buffer and spun down immediately at 500 x g for 10 min at 4 C. Transposition reaction mix with the enzyme and transposition buffer from Illumina Nextera DNA Library Preparation Kit was added to the pellet and incubated for 30 min at 37 C. The DNA was purified after transposition using a Qiagen MinElute kit and eluted in 10ul of the provided elution buffer. Eluted after transposition DNA was PCR amplified for a total of 11-13 cycles using barcoded primers from Illumina Nextera XT Index Kit v2. DNA was purified and adapters were removed using Ampure beads (1.4:1.0 beads:sample ratio). The quality and concentration of the eluted libraries were determined using an Agilent Bioanalyzer HS chip and a Qubit fluorometer. Libraries were sequenced on Illumina NextSeq 500 in paired-end mode.
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: pericyte
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000669
characteristics.characteristic: progenitor cell type
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: 2FPCp25r2
characteristics.characteristic: title