characteristics.text: F-CCTACGGGAGGCAGCAG;R-GGACTACHVGGGTWTCTAAT
characteristics.characteristic: 16s_primer
characteristics.text: ERC000014
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-10-31T04:04:09Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-16T20:47:41Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6087707
characteristics.characteristic: External Id
characteristics.text: MPIEVB
characteristics.characteristic: INSDC center name
characteristics.text: 2019-10-31T04:04:09Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-16T20:47:41Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3888113
characteristics.characteristic: SRA accession
characteristics.text: G05112-L1
characteristics.characteristic: Submitter Id
characteristics.text: 1 �l
characteristics.unit: L
characteristics.characteristic: amount or size of sample collected
characteristics.text: V3V4-two step
characteristics.characteristic: amplification protocol
characteristics.text: 2016-08-02
characteristics.characteristic: collection date
characteristics.text: Mock community
characteristics.characteristic: common name
characteristics.text: ZymoBIOMICS� Microbial Community DNA Standard (Lot.: ZRC187324, ZRC187325) consisting of 8 bacterial species (Pseudomonas aeruginosa (10.4%), Escherichia coli (9.0%), Salmonella enterica (11.8%), Lactobacillus fermentum (10.3%), Enterococcus faecalis (14.1%), Staphylococcus aureus (14.6%), Listeria monocytogenes (13.2%), Bacillus subtilis (13.2%)) and two fungi (Saccharomyces cerevisiae (1.6%), Cryptococcus neoformans (1.8%))
characteristics.characteristic: description
characteristics.text: artificial
characteristics.characteristic: environment
characteristics.text: mock community
characteristics.characteristic: environment (biome)
characteristics.text: mock community
characteristics.characteristic: environment (feature)
characteristics.text: mock community
characteristics.characteristic: environment (material)
characteristics.text: USA
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 33.686263
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: -117.830523
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: Irvine, California, USA
characteristics.characteristic: geographic location (region and locality)
characteristics.text: MOCK10
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001461
characteristics.characteristic: host subject id
characteristics.text: miscellaneous natural or artificial environment
characteristics.characteristic: host-associated environmental package
characteristics.text: human-associated
characteristics.characteristic: human associated environmental package
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: PCR
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000415
characteristics.characteristic: library construction method
characteristics.text: i5-AAGGCTAT;i7-TTCTGCCT
characteristics.characteristic: multiplex identifiers
characteristics.text: DOI:10.1186/s40168-019-0743-1
characteristics.characteristic: nucleic acid extraction
characteristics.text: synthetic metagenome
characteristics.characteristic: organism
characteristics.text: initial denaturation:98degC_30s;30times;denaturation:98degC_9s;annealing:55degC_60s;extension:72degC_90s;final extension:72degC_10min;storage:10degC_infinity
characteristics.characteristic: pcr conditions
characteristics.text: step 1 F-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTGCGATCCCTACGGGAGGCAGCAG;step 1 R-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTCTCGGACTACHVGGGTWTCTAAT;step 2 F-AATGATACGGCGACCACCGAGATCTACACAAGGCTATTCGTCGGCAGCGTC;step 2 R-CAAGCAGAAGACGGCATACGAGATTTCTGCCTGTCTCGTGGGCTCGG
characteristics.characteristic: pcr primers
characteristics.text: Comparative analysis of amplicon and metagenomic sequencing methods reveals key features in the evolution of animal metaorganisms
characteristics.characteristic: project name
characteristics.text: synthetic metagenome
characteristics.characteristic: scientific_name
characteristics.text: Illumina
characteristics.characteristic: sequencing method
characteristics.text: 16S rRNA
characteristics.characteristic: target gene
characteristics.text: V3V4
characteristics.characteristic: target subfragment
characteristics.text: Mock community
characteristics.characteristic: title