characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-06-12T04:06:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-17T07:18:38Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6087946
characteristics.characteristic: External Id
characteristics.text: IFREMER
characteristics.characteristic: INSDC center name
characteristics.text: 2020-06-12T04:06:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-17T07:18:38Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3888352
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8451:T1M4BR2_LH
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: In situ experiment: B. azoricus mussels were sampled under red light during the MOMARSAT 2017 cruise (July 16 and 17; doi 10.17600/17000500) at the Lucky Strike vent field of the Mid-Atlantic Ridge, on the NE part of the Eiffel Tower edifice, at a mean depth of 1688m, using the Remotely-Operated Vehicle (ROV) Victor6000. B. azoricus mussels (68 ± 14 mm shell length, mean ± sd) were sampled every 2h 4min for 24h 48min. For each sampling time, 7 to 15 mussels were placed in a separate box filled with in-house RNA stabilising solution to preserve RNA immediately upon sampling. Aboard, 7 mussels per time point were dissected at 4°C and tissues were placed in individual tubes in RNA later© (Sigma-Aldrich), kept at 4°C overnight then transferred to -80°C before further processing. Laboratory experiment: B. azoricus mussels were sampled during the Biobaz 2017 cruise (July 26) on the Menez Gwen vent field of the Mid-Atlantic Ridge, on the locality Albert de Monaco, White Flame area, at a mean depth of 834m, using the ROV Victor6000. A total of 108 mussels of similar sizes were collected for the laboratory experiment. Mussels were kept on board for 40h in natural seawater before being transferred to the Lab Horta facility of the Universidade dos Açores. Mussels were acclimated for 48h in an aquarium at 6.0 ± 0.5°C and pH 6.8 under a 12h:12h L:D schedule. Night sampling was performed under red light. A total of 65 B. azoricus mussels (63 ± 8 mm shell length, mean ± sd) were then sampled every 2h 4min for 24h 48min. Mussels’ tissues were dissected and stored in liquid N2 before further processing. Total RNA were isolated using Extract-all reagent (Eurobio, France) at a concentration of 1 ml.30 mg-1 tissue, treated with DNAse I (DNAse Max Kit, Qiagen, Germany) and assayed for concentration and quality. All samples complied with purity criteria (OD260/OD280 and OD260/OD230 > 1.8) and quality criteria (RNA integrity). For the field experiment, 5 out of 7 animals were selected for each time point for further sequencing, making a total of 65 samples for the in situ experiment. For the lab experiment, all 65 samples complied with quality control criteria and were therefore used for sequencing. The cDNA libraries were built for each sample using the TruSeq Stranded mRNA Sample Prep kit v2 (Illumina, California, USA) according to manufacturer instructions. mRNA were purified using poly-(T) beads from 3 µg of each total RNA sample
characteristics.characteristic: description
characteristics.text: laboratory experiment
characteristics.characteristic: growth condition
characteristics.text: Bathymodiolus azoricus
characteristics.characteristic: organism
characteristics.text: Bathymodiolus azoricus
characteristics.characteristic: organism
characteristics.text: gill
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002535
characteristics.characteristic: organism part
characteristics.text: Bathymodiolus azoricus
characteristics.characteristic: scientific_name
characteristics.text: T1M4BR2_LH
characteristics.characteristic: title