characteristics.text: F-CCTACGGGAGGCAGCAG;R-GGACTACHVGGGTWTCTAAT
characteristics.characteristic: 16s_primer
characteristics.text: ERC000014
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-10-31T04:04:09Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-16T20:47:07Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6087657
characteristics.characteristic: External Id
characteristics.text: MPIEVB
characteristics.characteristic: INSDC center name
characteristics.text: 2019-10-31T04:04:09Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-16T20:47:07Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3888063
characteristics.characteristic: SRA accession
characteristics.text: G05102-L1
characteristics.characteristic: Submitter Id
characteristics.text: 1 worm
characteristics.unit: L
characteristics.characteristic: amount or size of sample collected
characteristics.text: V3V4-two step
characteristics.characteristic: amplification protocol
characteristics.text: 2012-07-04
characteristics.characteristic: collection date
characteristics.text: roundworm
characteristics.characteristic: common name
characteristics.text: L2 isolated from a slug from compost in the botanical garden, Kiel, Schleswig-Holstein
characteristics.characteristic: description
characteristics.text: terrestrial
characteristics.characteristic: environment
characteristics.text: temperate broadleaf forest biome
characteristics.characteristic: environment (biome)
characteristics.text: park
characteristics.characteristic: environment (feature)
characteristics.text: compost
characteristics.characteristic: environment (material)
characteristics.text: Germany
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 54.349100
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 10.115050
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: Schleswig-Holstein, Kiel, botanical garden
characteristics.characteristic: geographic location (region and locality)
characteristics.text: full body
characteristics.characteristic: host body habitat
characteristics.text: full body
characteristics.characteristic: host body product
characteristics.text: full body
characteristics.characteristic: host body site
characteristics.text: Celegans_2
characteristics.characteristic: host subject id
characteristics.text: host-associated
characteristics.characteristic: host-associated environmental package
characteristics.text: human-associated
characteristics.characteristic: human associated environmental package
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: PCR
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/OBI_0000415
characteristics.characteristic: library construction method
characteristics.text: i5-TCGACTAG;i7-CTAGTACG
characteristics.characteristic: multiplex identifiers
characteristics.text: DOI:10.1186/1472-6785-14-4
characteristics.characteristic: nucleic acid extraction
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: organism
characteristics.text: 1
characteristics.characteristic: organism count
characteristics.text: 1. step:initial denaturation:98degC_30s;25times;denaturation:98degC_10s;annealing:55degC_30s;extension:72degC_60s;final extension:72degC_10min;storage:10degC_infinity; 2. step:initial denaturation:98degC_30s;10times;denaturation:98degC_9s;annealing:55degCs;extension:72degC_60s;final extension:72degC_10min;storage:10degC_infinity???
characteristics.characteristic: pcr conditions
characteristics.text: step 1 F-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTCCCTACGGGAGGCAGCAG;step 1 R-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGATCGGACTACHVGGGTWTCTAAT;step 2 F-AATGATACGGCGACCACCGAGATCTACACTCGACTAGTCGTCGGCAGCGTC;step 2 R-CAAGCAGAAGACGGCATACGAGATCTAGTACGGTCTCGTGGGCTCGG
characteristics.characteristic: pcr primers
characteristics.text: Comparative analysis of amplicon and metagenomic sequencing methods reveals key features in the evolution of animal metaorganisms
characteristics.characteristic: project name
characteristics.text: DOI:10.1186/1472-6785-14-4
characteristics.characteristic: sample collection device or method
characteristics.text: DOI:10.1186/1472-6785-14-4
characteristics.characteristic: sample material processing
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: scientific_name
characteristics.text: Illumina
characteristics.characteristic: sequencing method
characteristics.text: 16S rRNA
characteristics.characteristic: target gene
characteristics.text: V3V4
characteristics.characteristic: target subfragment
characteristics.text: Caenorhabditis elegans associated microbial sample
characteristics.characteristic: title