characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-10-17T04:03:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-07T12:11:13Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6056669
characteristics.characteristic: External Id
characteristics.text: Centre for Integrative Genetics (CIGENE), Faculty of Biosciences (BIOVIT), Norwegian University of Life Sciences (NMBU), 1432, As, Norway
characteristics.characteristic: INSDC center name
characteristics.text: 2019-10-17T04:03:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-07T12:11:13Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3857801
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8284:EM18
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Young adult worms were collected from NGM-Petri dishes. The external gamma radiation exposure was conducted at the FIGARO 60Co irradiation facility (activity ~420 GBq) at the Norwegian University of Life Sciences (NMBU) (Lind et al., 2019). Nematodes were exposed, in triplicate, in liquid media (15 ml tissue-culture flasks or front row 24-well cell culture plates) or on NGM-Petri dishes. During exposure, controls were placed, in triplicate, behind lead shielding, while exposure containers were placed at distances corresponding to a calculated average absorbed dose-rates to water of 0.43 - 1.1 - 10.8 - 40.8 and 99.9 mGy/h. RNA was extracted after 72 hours of exposure from L1 stage on young adult nematodes (n=1000 per replicate, three biological replicates per treatment), nematodes were washed and snap-frozen in LIN (liquid nitrogen) and stored at -80 °C until used. Total RNA was extracted using Direct-zol Reagent (Nordic Biosite) and purified with RNeasy Mini Kit (Zymo Research) according to manufacture instruction. Strand-specific TruSeq™ RNA-seq paired-end libraries were prepared according to manufacture instruction.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: organism
characteristics.text: Caenorhabditis elegans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6239
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organism part
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: scientific_name
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: N2
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004028
characteristics.characteristic: strain
characteristics.text: EM18
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002176
characteristics.characteristic: title