characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-02-06T17:04:58Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-15T14:34:57Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6086278
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF MANCHESTER
characteristics.characteristic: INSDC center name
characteristics.text: 2020-02-06T17:04:58Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-15T14:34:57Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3886686
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8429:Sample 8
characteristics.characteristic: Submitter Id
characteristics.text: 9
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: After treatment mice were sacrificed after 10 days and both kidneys were harvested with the right kidney acting as control tissue. The Sox9fl/fl;RosaCreER mice have been described previously (PMID: 29109128). Adult (8 week) male mice were used and genotyping was performed from DNA prepared from ear clips following our published protocol for these animals. Mice were housed and maintained, and animal experiments performed under approval from the University of Manchester Ethical Review Committee and UK Government Home Office licence for animal research. For genetic recombination of Sox9fl/fl;RosaCreER mice 120mg/kg Tamoxifen was injected intraperitoneally (i.p.) three times in the week prior to kidney fibrosis induced by UUO. Under isoflurane anaesthesia, the ureter of the left kidney was isolated via central laparotomy and sutured closed in 2 places to induce renal fibrosis. A maintenance dose of 60mg/kg Tamoxifen was given 1 week post UUO. Mice were sacrificed after 10 days and both kidneys were harvested with the right kidney acting as control tissue. All randomly assigned experimental and controls were littermate sex- and age-matched mice. RNA was isolated using the RNeasy kit (Qiagen). Truseq stranded mRNA Assay. Paired end.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: developmental stage
characteristics.text: fibrosis
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006890
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: 4
characteristics.characteristic: individual
characteristics.text: unilateral ureteral obstruction
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005562
characteristics.characteristic: injury
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: kidney
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002113
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: Sample 8
characteristics.characteristic: title