characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-07-06T04:03:41Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-08T10:56:39Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6059155
characteristics.characteristic: External Id
characteristics.text: 3. Department of General Surgery, Shanghai Sixth People’s Hospital East Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai, 201308, China
characteristics.characteristic: INSDC center name
characteristics.text: 2020-07-06T04:03:41Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-08T10:56:39Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3860284
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8417:mutant_2_stat3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: SW480
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002083
characteristics.characteristic: cell line
characteristics.text: epithelial cell of large intestine
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002253
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: SW480 cells were edited by CRISPR-CAS9 for the residue 400 to 411 (\NNGSLSAEFKHL\) of Stat3 deletion. 10% whole cell lysates were saved as input after genomic DNA was broken into 200-500 bp by sonication. 1 μg IP grade antibodies of STAT3 were incubated with the rest of the lysate overnight, followed by 2 h protein-A beads incubation at 37 °C for target protein pull down. The STAT3 enriched DNA or input DNA were repaired to 3’-dA overhang and added the ligated adapter. 0.2 ug of pull down DNA sample was submitted to NOVOGENE (Beijing, China) for library generation and sequencing.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: colorectal carcinoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000365
characteristics.characteristic: disease
characteristics.text: STAT3 knockout
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: colon
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: mutant_2_stat3
characteristics.characteristic: title