characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-10-15T04:05:16Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-14T16:30:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6082039
characteristics.characteristic: External Id
characteristics.text: Bering Limited
characteristics.characteristic: INSDC center name
characteristics.text: 2019-10-15T04:05:16Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-14T16:30:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3882453
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-73451571066817:8-0_ct_ev_but_S27
characteristics.characteristic: Submitter Id
characteristics.text: 6 to 12
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: B cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000236
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Splenic CD21hiCD24hi B cells were isolated from Mb1cre and Ahrfl/-Mb1cre mice in the remission phase of arthritis. Sorted cells were either left untouched (ex-vivo) or stimulated with LPS+anti-IgM for 6h in IMDM media. Dead cells were excluded using DAPI. Butyrate: 150mM; LPS: 10 mg/ml; IgM; 10 ug/ml Total RNA was isolated from these populations using the Picopure RNA isolation kit (ThermoFisher Scientific), according to manufacturer’s instructions. 130-500ng of total RNA was fragmented followed by reverse transcription and second strand cDNA synthesis. 130-500ng of total RNA was fragmented followed by reverse transcription and second strand cDNA synthesis. The double strand cDNA was subjected to end repair, A base addition, adapter ligation and PCR amplification to create libraries. Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of samples in 3 lanes.
characteristics.characteristic: description
characteristics.text: Mb1+/cre
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: spleen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0001281
characteristics.characteristic: organism part
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: not available
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001071
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: 8-0_ct_ev_but_S27
characteristics.characteristic: title