characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-01-21T17:08:23Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-11T16:58:03Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6066414
characteristics.characteristic: External Id
characteristics.text: Oxford Nanopore Technologies
characteristics.characteristic: INSDC center name
characteristics.text: 2020-01-21T17:08:23Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-11T16:58:03Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3867534
characteristics.characteristic: SRA accession
characteristics.text: SIRV_E0_1
characteristics.characteristic: Submitter Id
characteristics.text: Synthetic spike-in transcripts made by Lexogen (SIRV E0): https://www.lexogen.com/store/sirvs SIRV E0 polyA RNA (Lexogen) (1ng) was used as a template for reverse transcription for use in the PCS-109 cDNA by PCR sequencing kit (Oxford Nanopore) Following the manufacturer’s instructions (https://community.nanoporetech.com/protocols/cdna-pcr-sequencing_sqk-pcs109/v/PCS_9085_v109_revJ_14Aug2019). For amplification of the first strand cDNA, 12 cycles were used and 100 fM of library was loaded onto a FLO-MIN106 flowcell and sequenced for 48 hrs on the GridION system. Basecalling was performed in real time using guppy 3.4.8. Only a subset of pass reads with mean base quality larger than 7 were uploaded.
characteristics.characteristic: description
characteristics.text: synthetic RNA
characteristics.characteristic: organism
characteristics.text: synthetic RNA
characteristics.characteristic: scientific_name
characteristics.text: Lexogen SIRV E0 PCS109
characteristics.characteristic: title