characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-09-30T04:05:07Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-07T18:06:38Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6057278
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF MANCHESTER
characteristics.characteristic: INSDC center name
characteristics.text: 2020-09-30T04:05:07Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-07T18:06:38Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3858410
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8402:Reverba_WT_liver_rep2
characteristics.characteristic: Submitter Id
characteristics.text: 13
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Gonadal white adipose tissue (gWAT) or liver tissue was collected from adult male Reverbαflox/floxAdipoCre+/-, Reverbαflox/floxAlbCreERT2+/-, Reverbα-/- global knockout (KO) or Reverbα wildtype mice (littermates of global knockouts) at ZT8 and flash frozen (n=6-8). Frozen tissue was homogenised in TRIzol Reagent (Invitrogen) using Lysing Matrix D tubes (MP Biomedicals) and total RNA extracted according to the manufacturer’s TRIzol protocol as far as the isopropanol phase, at which point samples were transferred to columns from the ReliaPrep RNA Miniprep Tissue Kit (Promega) to ensure high quality RNA. The column was then washed, DNAse treated and RNA eluted as per Promega’s protocol. Biological replicates were taken forward individually. Total RNA was submitted to the University of Manchester Genomic Technologies Core Facility (GTCF). Quality and integrity of the RNA samples were assessed using a 2200 TapeStation (Agilent Technologies). Illumina TruSeq Stranded mRNA kit, according to the manufacturer’s protocol
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: liver
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002107
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004472
characteristics.characteristic: strain
characteristics.text: Reverba_WT_liver_rep2
characteristics.characteristic: title