characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-05-01T04:03:29Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-22T10:00:06Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6106823
characteristics.characteristic: External Id
characteristics.text: CGR, Institute of Integrative Biology, The University of Liverpool, UK.
characteristics.characteristic: INSDC center name
characteristics.text: 2020-05-01T04:03:29Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-22T10:00:06Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3907501
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8458:Sample 59
characteristics.characteristic: Submitter Id
characteristics.text: 24
characteristics.unit: month
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: common carp
characteristics.characteristic: common name
characteristics.text: Protocols: 6 month old Common Carp (Cyprinus carpio, L.) were obtained from Norton Aquaculture (Litchfield, UK), from a single mating of their broodstock. On transfer to the Liverpool Aquarium Facility the fish (300-400g, previously reared at 21-23°C) were maintained at 25+/-0.2°C, under a 14h photoperiod, for 4 months in a 2000L fibreglass tank (Purewell, Droxford, UK).Fish from each tank were randomly sampled at each of the desired timepoints, killed, and tissues rapidly excised and frozen in liquid nitrogen followed by storage at -80°C. 3 weeks prior to the cooling experiment the 25°C pre-conditioned carp were randomly separated into two groups. Pre-conditioned carp were randomly separated into two groups, (i) a control group of 30 fish held throughout at 25°C, and (ii) 70 fish that were subjected to a controlled 7°C cooling (1°C/h, 0900-1600h) over each of two successive days down to 11°C, at which they were held for up to 24 days. RNA was extracted from ~10 mg kidney tissue using Animal Tissue Purification kit (Norgen Biotek Corp.). Samples were homogenised in 300µL Buffer RLT with beta-mercaptoethanol using Tissuelyser II and extraction was performed according to the manufacturer’s instructions which included DNase I treatment (Qiagen). RNA was used as template for NEBNext® UltraDirectional RNA Library Prep Kit for Illumina (E7420L NEB) performed as per the manufacturers’ instructions.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: 170
characteristics.characteristic: individual
characteristics.text: Cyprinus carpio
characteristics.characteristic: organism
characteristics.text: Cyprinus carpio
characteristics.characteristic: organism
characteristics.text: kidney
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002113
characteristics.characteristic: organism part
characteristics.text: Cyprinus carpio
characteristics.characteristic: scientific_name
characteristics.text: Sample 59
characteristics.characteristic: title