characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2021-06-01T00:11:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2021-06-01T00:11:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA5860548
characteristics.characteristic: External Id
characteristics.text: Universidade Federal do Parana (UFPR)
characteristics.characteristic: INSDC center name
characteristics.text: 2021-06-01T00:11:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2021-06-01T00:11:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3649049
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8238:wt +NH4 02
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Cells were collected and stored in RNAlater stabilization solution (Thermo Fisher Scientific). Cells were cultivated in NFbHP-malate supplemented with 1 mM NH4Cl, at 30C and 250 rpm. When cells reached OD600 = 0.4, cells were collected and stored in RNAlater. Additionally, the remaining cells were incubated for a further 30 minutes after addition of 10 mM NH4Cl (Ammonium shock) or 10 mM KNO3 (Nitrate shock). After incubation, cells were collected and stored in RNAlater. RNA was extracted using Trizol (Thermo Fisher Scientific), and contaminant DNA was removed using TURBO DNase (Thermo Fisher Scientific). rRNA was removed using Ribo-Zero rRNA Removal kit (Gram-negative bacteria) (Illumina). cDNA libraries were constructed using Ion Total RNASeq kit v2 (Thermo Fisher Scientific).
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: NFbHP-malate supplemented with 1 mM NH4Cl
characteristics.characteristic: growth condition
characteristics.text: Herbaspirillum seropedicae SmR1
characteristics.characteristic: organism
characteristics.text: Herbaspirillum seropedicae SmR1
characteristics.characteristic: organism
characteristics.text: Herbaspirillum seropedicae SmR1
characteristics.characteristic: scientific_name
characteristics.text: wt +NH4 02
characteristics.characteristic: title