characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-12-02T04:02:53Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-16T14:56:03Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6087124
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF CAMBRIDGE
characteristics.characteristic: INSDC center name
characteristics.text: 2019-12-02T04:02:53Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-16T14:56:03Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3887531
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8442:Glycerol_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Pseudmonas aeruginosa PAO1 wild-type cultured in MOPS Glycerol compared to MOPS Glycerol Hypoxia (restricted oxygen). P. aeruginosa strain PAO1 was grown in 40 ml MOPS with glycerol as sole carbon source (triplicate), 37 °C with shaking (250 rpm) in baffled flasks (500 ml). For the restricted oxygen condition, P. aerugionosa was cultured in the same conditions, except non-baffled flasks were used, and the shaking speed was 80 rpm (gentle aggitation). A thin layer 10 ml of mineral oil was overlaid on top of these cultures to restrict oxygen transfer. Total RNA was isolated as described in [90], followed by phenol-chloroform-isoamyl alcohol extraction and ethanol precipitation. Purity and concentration of the DNase treated (Turbo DNA-Free, Applied Biosystems) RNA was estimated with a NanoDrop ND-1000 spectrophotometer (NanoDrop technologies Inc, USA) and using agarose gel electrophoresis. Ribosomal RNA (rRNA) was then depleted from each sample (5 µg each) using the bacterial Ribo-Zero rRNA Removal Kit (Illumina). The integrity of the RNA was evaluated using an RNA 6000 Nano LabChip and an Agilent 2100 Bioanalyzer (Agilent Technologies, Germany). Six indexed, strand-specific cDNA libraries were prepared, and samples were sequenced on an Illumina HiSeq 2000 with a 51-bp single-end read length (GATC Biotech, Germany).
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: MOPS Glycerol
characteristics.characteristic: growth condition
characteristics.text: Pseudomonas aeruginosa PAO1
characteristics.characteristic: organism
characteristics.text: Pseudomonas aeruginosa PAO1
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organism part
characteristics.text: Pseudomonas aeruginosa PAO1
characteristics.characteristic: scientific_name
characteristics.text: Glycerol_2
characteristics.characteristic: title