characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-10-15T17:02:57Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-15T09:40:59Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6085998
characteristics.characteristic: External Id
characteristics.text: Bering Limited
characteristics.characteristic: INSDC center name
characteristics.text: 2019-10-15T17:02:57Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-15T09:40:59Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3886406
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-75251571128229:AhrKO_ExVivo_Butyrate_1
characteristics.characteristic: Submitter Id
characteristics.text: 8-12
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: B cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000236
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: FACS sorted subsets were either left untouched (Ex vivo) or cultured for 6h with 1mg/ml LPS and 10mg/ml anti-IgM, before DNA extraction. ~40,000 cells were pelleted by centrifuging for 5 min at 4 °C at 300 x g. After centrifugation, the pellet was carefully resuspended in the transposase reaction mix (12.5 ml 2xTD buffer, 2 ml TDE1 (Illumina) and 10.25 ml nuclease-free water, 0.25 µl 5% Digitonin (Sigma)) for 30 min at 37 °C. Per manufacturer's instruction Per manufacturer's instruction
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Ahrfl/-;Mb1+/cre
characteristics.characteristic: genotype
characteristics.text: 3-0_ko_ev_but
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: spleen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0001281
characteristics.characteristic: organism part
characteristics.text: CD19+CD21hiCD24hi
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: butyrate
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_17968
characteristics.characteristic: stimulus
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: AhrKO_ExVivo_Butyrate_1
characteristics.characteristic: title