characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2020-04-14T16:27:57Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-07T12:03:54Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6056647
characteristics.characteristic: External Id
characteristics.text: Molecular Digestive Oncology, Department of Oncology, University of Leuven
characteristics.characteristic: INSDC center name
characteristics.text: 2020-04-14T16:27:57Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-07T12:03:54Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3857780
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-8412:Sample2b
characteristics.characteristic: Submitter Id
characteristics.text: 86
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Resection samples were cut open and rinsed with water to remove any remaining stool. Two samples from each tumor specimen were collected corresponding to tumor core and invasive margin (tumor border). The samples were then snap frozen in liquid nitrogen and subsequently stored in -80oC for long term storage. RNA isolation was performed with Trizol (15596026, Invitrogen) using the protocol recommended by the manufacturer. For nucleic acid library construction, nKAPA Stranded mRNA-Seq Kit (KR0960 - v5.17, KAPABiosystems) was used according to the manufacturer recommendations.
characteristics.characteristic: description
characteristics.text: adult
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: developmental stage
characteristics.text: colorectal cancer
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005842
characteristics.characteristic: disease
characteristics.text: SC019
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006481
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: colon
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001155
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Sample2b
characteristics.characteristic: title