characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-10-07T17:04:03Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2019-10-07T11:32:09Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA6056645
characteristics.characteristic: External Id
characteristics.text: Laboratory of Molecular Medicine and Genomics
characteristics.characteristic: INSDC center name
characteristics.text: 2019-10-07T17:04:03Z
characteristics.characteristic: INSDC first public
characteristics.text: 2019-10-07T11:32:09Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS3857778
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-68921570443897:Sample 7
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: MCF-7
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001203
characteristics.characteristic: cell line
characteristics.text: mammary epithelial cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002327
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: ER-alpha positive BC cells: MCF-7 (ATCC HTB-22),were propagated in Dulbecco’s modified Eagle medium (DMEM; Sigma-Aldrich) supplemented with 10% FBS (HyClone) and antibiotics: 100 U/ml penicillin, 100 mg/ml streptomycin, 250 ng/ml amphotericin-B. MCF-10A (ATCC CRL-10317) was cultured in Mammary Epithelial Cell Growth Medium (MEGM BulletKit, Lonza, Catalog No. CC-3150) with added 100 ng/ml cholera toxin. Tamoxifen resistant MCF-7/LCC2 cells (referred to as LCC2) were kindly provided by Prof. Janne Lehtio, Department of Oncology-Pathology, Karolinska Institutet, Sweden.tamoxifen resistanat cells were maintained DMEM supplemented as described above with the addiction of 1 µM 4-hydroxytamoxifen (4-OHTAM) (Sigma-Aldrich) Total RNA extraction was performed by using TRIzol (Invitrogen) as previously described in Nassa et al, 2018. RNA concentration in each sample was assayed Quant-IT RNA Assay Kit-High Sensitivity and a Qubit Fluorometer (Life Technologies) and its quality and integrity assessed with Agilent RNA ScreenTape & Reagents using an Agilent 4200 TapeStation System (Agilent Technologies). mRNA sequencing experiments, indexed libraries were prepared using 1 µg of total RNA from MCF-7 treated with 4-OHT 10-7 M or vehicle for 3 days, with TruSeq Stranded Total mRNA Sample Prep Kit (Illumina Inc.)
characteristics.characteristic: description
characteristics.text: invasive ductal carcinoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000186
characteristics.characteristic: disease
characteristics.text: pleural effusion
characteristics.characteristic: metastatic site
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: mammary gland
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0000817
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 7
characteristics.characteristic: title