characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2021-09-16T13:25:58Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2021-09-16T13:25:58Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10109862
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF NEW MEXICO
characteristics.characteristic: INSDC center name
characteristics.text: 2021-09-16T13:25:58Z
characteristics.characteristic: INSDC first public
characteristics.text: 2021-09-16T13:25:58Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7766420
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-5742:Sample 2(SCI045144-2)
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: microglial cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000129
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Human microglial cells (ScienCell, Cat. #: 1900) were thawed and plated in a 6-well tissue culture plate pre-coated with 0.1mg/ml of Poly-L-Lysine (Sigma, P4707). The culture media was changed 16 hours after thawing and cells remained undisturbed for another 24hours. Human microglial cells were treated with vehicle or 2ug/ml of hPHF (paired helical filaments isolated from human AD brain) in triplicates for 24 hours. RNA was extracted from cells using Qiagen Rneasy mini kit with DNase treatment as per manufacturer's instructions. Library construction was performed by Quick Biology Inc. (Pasadena, CA) using KAPA Stranded RNA-Seq Kits with RiboErase.
characteristics.characteristic: description
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 2(SCI045144-2)
characteristics.characteristic: title