characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2021-09-25T00:40:37Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2021-09-25T00:40:37Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10160204
characteristics.characteristic: External Id
characteristics.text: Department of Neurological Surgery Washington University in St. Louis
characteristics.characteristic: INSDC center name
characteristics.text: 2021-09-25T00:40:37Z
characteristics.characteristic: INSDC first public
characteristics.text: 2021-09-25T00:40:37Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7816688
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-9435_fastq:GBM2_total_3p_GEX_CR3
characteristics.characteristic: Submitter Id
characteristics.text: 69
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: glioblastoma and infiltrating normal cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cell dissociation and tissue processing: Fresh tumor samples were dissociated using the gentleMACS Octo Dissociator and Brain tumor dissociation kit (Miltenyi). Myelin and red blood cells were removed using the MACS Myelin removal beads II and ACK Red Blood Cell lysis buffer, respectively. Dead cells were removed (MACS Dead cell removal microbeads) and viable cells were counted by Trypan blue exclusion. Fresh tumor samples were dissociated using the gentleMACS Octo Dissociator and Brain tumor dissociation kit (Miltenyi). Myelin and red blood cells were removed using the MACS Myelin removal beads II and ACK Red Blood Cell lysis buffer, respectively. Dead cells were removed (MACS Dead cell removal microbeads) and viable cells were counted by Trypan blue exclusion. Dissociated tumor cells were processed using the 10x Genomics Chromium Controller and the Chromium Single Cell 3′V2 Library & Gel Bead Kit following the manufacturer's protocols (https://tinyurl.com/ybpg2pfz). Dissociated tumor cells were processed using the 10x Genomics Chromium Controller and the Chromium Single Cell 3′V2 Library & Gel Bead Kit following the manufacturer's protocols (https://tinyurl.com/ybpg2pfz).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: glioblastoma multiforme
characteristics.characteristic: disease
characteristics.text: unsorted
characteristics.characteristic: immunophenotype
characteristics.text: B150
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: brain
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: GBM2_total_3p_GEX_CR3
characteristics.characteristic: title