characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2021-10-08T01:08:04Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2021-10-08T01:08:04Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10086401
characteristics.characteristic: External Id
characteristics.text: University of Padova - TESAF Department
characteristics.characteristic: INSDC center name
characteristics.text: 2021-10-08T01:08:04Z
characteristics.characteristic: INSDC first public
characteristics.text: 2021-10-08T01:08:04Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7743057
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10927:Sample 7
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Conidia of P. oryzae (IT10 strain) were cultured in 1.8 mL Complete Medium (CM; Talbot et al., 1993) at a final concentration of 2x105 conidia per mL for 3 days at 30 °C under shaking at 150 rpm. After three days, 50 µM of Pep 4rink, one of the most effective peptides in vivo, were added to samples containing actively growing mycelium. Untreated samples were used as control. Three h after peptide treatment, fungal mycelia were collected, filtered with 100 µm Cell Strainer (Corning®), rinsed with sterile water and stored at -80 °C for RNA extraction. Total RNA was extracted with the RNeasy Plant Mini kit (Qiagen) following the manufacturer’s instructions, performing the DNase digestion directly on the column. Two µg of total RNA were subjected to PolyA tail capture and rRNA depletion for selection of mRNA. Directional (stranded) RNA libraries were prepared, quantified and subjected to quality control. Sequencing was performed on Illumina Nova Seq 6000 platform.
characteristics.characteristic: description
characteristics.text: complete medium
characteristics.characteristic: growth condition
characteristics.text: Pyricularia oryzae 70-15
characteristics.characteristic: organism
characteristics.text: Pyricularia oryzae 70-15
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_318829
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organism part
characteristics.text: Pyricularia oryzae 70-15
characteristics.characteristic: scientific_name
characteristics.text: IT10
characteristics.characteristic: strain
characteristics.text: Sample 7
characteristics.characteristic: title