characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2021-12-06T00:11:46Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2021-12-06T00:11:46Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10112510
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF CAMBRIDGE
characteristics.characteristic: INSDC center name
characteristics.text: 2021-12-06T00:11:46Z
characteristics.characteristic: INSDC first public
characteristics.text: 2021-12-06T00:11:46Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7769066
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-93491631874715:SLX.12168.N701_S504.C9JGJANXX
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Neonate forebrain-derived cells
characteristics.characteristic: cell line
characteristics.text: neonate forebrain-derived cell
characteristics.characteristic: cell type
characteristics.text: white-tufted-ear marmoset
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were dissociated in Accutase for 5 min and pipetted vigorously to obtain a single cell suspension in normal culture media. Single cells were picked by mouth pipette using a blunted microcapillary with internal diameter 15 μM and under a dissection microscope. Cells were collected into 0.2 mL tubes with 4 μL lysis buffer and immediately snap frozen on dry ice. RNA separation was performed using biotinylated oligo-dT30VN-tailed oligonucleotides (IDT) conjugated to Dynabeads Streptavidin C1 in an RNAse-inhibitor supplemented buffer solution. Sequencing libraries were prepared according to an adapted version of the G&T protocol previously described. Samples were transferred to a 96-well plate and prepared for both transcriptome and methylome sequencing, by physical separation of genomic DNA (gDNA) from messenger RNA (mRNA).
characteristics.characteristic: description
characteristics.text: neonate
characteristics.characteristic: developmental stage
characteristics.text: Callithrix jacchus
characteristics.characteristic: organism
characteristics.text: Callithrix jacchus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9483
characteristics.characteristic: organism
characteristics.text: frontal lobe
characteristics.characteristic: organism part
characteristics.text: pass
characteristics.characteristic: post analysis well quality
characteristics.text: Callithrix jacchus
characteristics.characteristic: scientific_name
characteristics.text: OK
characteristics.characteristic: single cell well quality
characteristics.text: SLX.12168.N701_S504.C9JGJANXX
characteristics.characteristic: title