characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-01-21T00:22:10Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-01-21T00:22:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10838352
characteristics.characteristic: External Id
characteristics.text: University of Cambridge
characteristics.characteristic: INSDC center name
characteristics.text: 2022-01-21T00:22:10Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-01-21T00:22:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8489586
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11135:PreME
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: H1-hESC
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0003042
characteristics.characteristic: cell line
characteristics.text: Pre-mesendoderm
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: hESCs, PreME and ME cells were dissociated using TrypLE, neutralised using MEF media and sorted to remove doublets and debries using a SONY SH800 sorter hESCs were cultured using E8 media on vitronectin coated plates. PreME and ME cells were grown according to Kobayashi et al 2017. PreME/ME protocols were optimised based on previous conditions. For the basal medium, we used aRB27 medium which is composed of Advanced RPMI 1640 Medium (Thermo Fisher Scientific) supplemented with 1% B27 supplement (Thermo Fisher Scientific), 0.1 mM NEAA, 100 U/ml Penicillin- 0.1 mg/ml Streptomycin, 2 mM L-Glutamine. For PreME/ME induction, trypsinized Conv-hPSC were seeded on vitronectin-coated dish at 200,000 cells/well in 12 well plate and cultured in PreME/ME induction medium for 6-36 h as indicated in the figures. PreME/ME induction medium was composed of aRB27 medium supplemental with 100 ng/ml Activin-A (SCI), 3 μM GSK3i and 10 μM of ROCKi. Single Cell 3' 10X V2 Single Cell 3' 10X V2
characteristics.characteristic: description
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: blastocyst
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000358
characteristics.characteristic: organism part
characteristics.text: embryonic stem cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002322
characteristics.characteristic: progenitor cell type
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: PreME
characteristics.characteristic: title