characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2021-12-28T04:10:28Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2021-12-28T04:10:28Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10273702
characteristics.characteristic: External Id
characteristics.text: Latvian Biomedical Research and Study Centre
characteristics.characteristic: INSDC center name
characteristics.text: 2021-12-28T04:10:28Z
characteristics.characteristic: INSDC first public
characteristics.text: 2021-12-28T04:10:28Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7928559
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11020:RAT12-Pre
characteristics.characteristic: Submitter Id
characteristics.text: 12
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Norway rat
characteristics.characteristic: common name
characteristics.text: Protocols: After 4 weeks of regular exercise, rats underwent brief anesthesia and of blood from the tail vein was taken and immediately put into K3 EDTA tubes and centrifuged for 15 min at 2000 x g at 4 degrees C. This step was repeated for the same animals immediately after 1h of running. On the same day, a blood sample was collected from the sedentary rats following the same protocol. Plasma was collected and immediately frozen for further EV isolation. RNA was extracted from plasma EVs using miRNeasy micro kit (Qiagen, USA) according to the manufacturer’s protocol. The concentration and quality of the obtained RNA were analyzed on Agilent Bioanalyzer with Agilent RNA 6000 Pico chip (Agilent Technologies, Germany). RNA libraries were constructed using CleanTag® Small RNA Library Prep Kit (Trilink Biotechnologies, USA), the quality and concentration of obtained libraries were analyzed on Agilent Bioanalyzer using Agilent High Sensitivity DNA chip (Agilent Technologies, Germany). The libraries were cleaned using Blue Pippin DNA Size Selection with 3% gel Blue Pippin Cassette (Sage Science, USA) setting tight target length to 140 bp thus selecting fragments with size in tight range to 140 bp (133–147 bp).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: trained on running wheel
characteristics.characteristic: environmental history
characteristics.text: extracellular vesicular exosome
characteristics.characteristic: fraction
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: RAT12
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Rattus norvegicus
characteristics.characteristic: organism
characteristics.text: Rattus norvegicus
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: before exercise
characteristics.characteristic: sampling time point
characteristics.text: Rattus norvegicus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: F344
characteristics.characteristic: strain
characteristics.text: RAT12-Pre
characteristics.characteristic: title