characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-01-21T00:15:20Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-01-21T00:15:20Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11043285
characteristics.characteristic: External Id
characteristics.text: Center for Biotechnology, Bielefeld University
characteristics.characteristic: INSDC center name
characteristics.text: 2022-01-21T00:15:20Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-01-21T00:15:20Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8693006
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11162:Sample 14
characteristics.characteristic: Submitter Id
characteristics.text: 8 to 10
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: golden hamster
characteristics.characteristic: common name
characteristics.text: Protocols: Male and female golden hamsters (8-10 weeks old) were purchased from Janvier. All animals were kept under standard housing conditions (21 ± 2 °C, 40-50% humidity, food and water ad libitum) with a 12:12 light-dark cycle at the Leibniz Institute for Experimental Virology in Hamburg, Germany. For SARS-CoV-2 infection, hamsters were anaesthetized with 150 mg/kg ketamine and 10 mg kg-1 xylazine by intraperitoneal injection. Male and female golden hamsters (8-10 weeks old) were purchased from Janvier. All animals were kept under standard housing conditions (21 ± 2 °C, 40-50% humidity, food and water ad libitum) with a 12:12 light-dark cycle at the Leibniz Institute for Experimental Virology in Hamburg, Germany. For SARS-CoV-2 infection, hamsters were anaesthetized with 150 mg/kg ketamine and 10 mg kg-1 xylazine by intraperitoneal injection. For letrozole treatment, male and female hamsters received a final dose of 0.36 mg kg-1 d-1 or 0.18 mg kg-1 d-1 letrozole (dissolved in DMSO, Sigma-Aldrich, L6545) diluted in sterile saline (Braun), respectively, by oral gavage (stainless-steel gavaging needles) at 3 h and day 1 to 3 post infection (p.i.). From day 4 to 8 p.i., hamsters received letrozole via the drinking water as water gel packages (Necta) supplemented with 1% glucose and a final concentration of 0.0018 mg ml-1 or 0.0036 mg ml-1 letrozole for female and male hamsters, respectively. As control, hamsters were treated with DMSO as vehicle. Viral RNA was isolated from homogenized organs using the QIAamp Viral RNA Mini Kit (QIAGEN) according to the manufacturer’s instructions. Preparation of stranded cDNA libraries was performed with Illumina Stranded mRNA Prep, Ligation kit, starting with 1000 ng total RNA according to the manufacturer’s instructions.
characteristics.characteristic: description
characteristics.text: coronavirus infectious disease
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Mesocricetus auratus
characteristics.characteristic: organism
characteristics.text: Mesocricetus auratus
characteristics.characteristic: organism
characteristics.text: lung
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002048
characteristics.characteristic: organism part
characteristics.text: Mesocricetus auratus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Syrian hamster
characteristics.characteristic: strain
characteristics.text: Sample 14
characteristics.characteristic: title