characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2021-12-31T00:11:40Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2021-12-31T00:11:40Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10257868
characteristics.characteristic: External Id
characteristics.text: Institute of Medical Sciences, School of Medicine, Medical Sciences & Nutrition, University of Aberdeen
characteristics.characteristic: INSDC center name
characteristics.text: 2021-12-31T00:11:40Z
characteristics.characteristic: INSDC first public
characteristics.text: 2021-12-31T00:11:40Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7912573
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11013:Ket rtt106-2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: For RNA-seq, cells were collected by centrifugation at 4,000 x g for 4 min. For RNA-seq analysis, cells were cultivated in YPD at 30 degrees Celsius and collected when they were in log phase growth. For ketoconazole treatment, cells grown in YPD overnight were pelleted and washed with water, resuspend in YEP to an OD600 of 0.6, incubated for 24 hours at 30 degrees Celsius, and then treated with 40 micro M ketoconazole for 15 min. at 30 degrees Celsius. For RNA-seq analysis, cells were cultivated in YPD at 30 degrees Celsius and collected when they were in log phase growth. For ketoconazole treatment, cells grown in YPD overnight were pelleted and washed with water, resuspend in YEP to an OD600 of 0.6, incubated for 24 hours at 30 degrees Celsius, and then treated with 40 micro M ketoconazole for 15 min. at 30 degrees Celsius. For RNA-seq, cells were disrupted using a bead beater and then total RNA was prepared using Macherey-Nagel NucleoSpin RNA column according to the manufacturer’s protocol. ERCC (External RNA Controls Consortium) spike in controls (Baker et al., 2005) were added to the RNA samples to be used as internal controls. Libraries were prepared with the Illumina TruSeq Stranded mRNA kit as per manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: CBS138 Cgrtt106∆::NAT
characteristics.characteristic: genotype
characteristics.text: glucose starvation (YEP) + ketoconazole
characteristics.characteristic: growth condition
characteristics.text: Candida glabrata
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5478
characteristics.characteristic: organism
characteristics.text: [Candida] glabrata
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organism part
characteristics.text: [Candida] glabrata
characteristics.characteristic: scientific_name
characteristics.text: VNG3
characteristics.characteristic: strain
characteristics.text: Ket rtt106-2
characteristics.characteristic: title