characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2021-12-31T00:11:39Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2021-12-31T00:11:39Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10107522
characteristics.characteristic: External Id
characteristics.text: Instituto Gulbenkian de Ciencia
characteristics.characteristic: INSDC center name
characteristics.text: 2021-12-31T00:11:39Z
characteristics.characteristic: INSDC first public
characteristics.text: 2021-12-31T00:11:39Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7764079
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10935:Pcc4
characteristics.characteristic: Submitter Id
characteristics.text: 15
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Whole livers of overnight fasted non-infected, Pcc infected (day 7 of infection and heme treated (15hrs after injection) C57BL6 mice were harvested, smashed into a soup, snap frozen in liquid nitrogen and stored at -80oC. We used fasted mice as controls to account for transcriptional changes owing to anorexia of infection, since heme injection and Pcc-infection induce anorexia. Total RNA was extracted from mouse tissues using tripleXtractor reagent (Grisp), chloroform (Merk) and with the NucleoSpin RNA kit (Machery-Nagel), while following the manufacturer’s instructions. We generated full-length cDNAs and sequencing libraries following the SMART-Seq2 protocol. After cDNA synthesis and QC check of cDNAs (Agilent Technologies), library preparation including ‘tagmentation’ of cDNA, PCR-mediated adaptor addition and amplification of the adapted libraries, was done following the Nextera library preparation protocol.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: fasting
characteristics.characteristic: diet
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: Pcc4
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_1148
characteristics.characteristic: title