characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2021-12-31T00:11:39Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2021-12-31T00:11:39Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10113320
characteristics.characteristic: External Id
characteristics.text: Bioinformatician
characteristics.characteristic: INSDC center name
characteristics.text: 2021-12-31T00:11:39Z
characteristics.characteristic: INSDC first public
characteristics.text: 2021-12-31T00:11:39Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7769875
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10943:Sample 27
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: BeWo
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002050
characteristics.characteristic: cell line
characteristics.text: trophoblast cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000351
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: RNA Sequencing was performed using BeWo choriocarcinoma cells. Cells were treated with the ER-stress induce, thapsigargin (concentration is 100 nM. As thapsigargin was dissolved in DMSO and was at 1 mM stock, therefore, 0.01% DMSO was added as vehicle control.) or vehicle in a paired design for 24 hour in serum-free modified DMEM/F12 medium containing 5.5 mM glucose. This was repeated using 5 independent replicate cultures (so 10 cultures in total). thapsigargin (Tg) with/without treatment for BeWo cells Nucleic acid extraction protocol: RNeasy Plus Mini Kit (catalog no. 74134; Qiagen) was used to isolate total RNA following manufacturer’s instruction. Illumina TruSeq Stranded mRNA Library Kit
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: choriocarcinoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002893
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: placenta
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001987
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 27
characteristics.characteristic: title