characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2021-12-31T00:11:44Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2021-12-31T00:11:44Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10589545
characteristics.characteristic: External Id
characteristics.text: Department of Experimental Plant Biology, Faculty of Science, Charles University
characteristics.characteristic: INSDC center name
characteristics.text: 2021-12-31T00:11:44Z
characteristics.characteristic: INSDC first public
characteristics.text: 2021-12-31T00:11:44Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8241465
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-102561635776307:_015_TRVWT_2_ub_2
characteristics.characteristic: Submitter Id
characteristics.text: 5
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: 16c
characteristics.characteristic: cultivar
characteristics.text: Protocols: Leaf disc (8 mm in diameter) was cut out from each infiltrated leaf (4th, 5th and 6th) and combined into a single biological sample 5 days post infiltration (dpi). Three samples were collected from each plant, frozen in liquid nitrogen and stored at 80 °C until further use. SANYO/Panasonic growth chambers with 16 h light 8 h dark photoperiods at 21 °C under 150 μmol·m−2·s−1 light Agrobacterium suspension (OD600=0.15 or 1.5) was infiltrated with a 1 ml syringe into the abaxial side of 3 leaves (4th 6th youngest leaves) of 5 week old N. benthamiana. DNA was isolated by the DNeasy Plant Mini Kit (QIAGEN). The target loci were amplified with the corresponding Illumina compatible primers, which were subsequently barcoded using the Nextera XT Index Kit V2 (Illumina).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: TRV_WT
characteristics.characteristic: epigenotype
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: SANYO/Panasonic growth chambers with 16 h light 8 h dark photoperiods at 21 °C under 150 μmol·m−2·s−1 light
characteristics.characteristic: growth condition
characteristics.text: TRV
characteristics.characteristic: infect
characteristics.text: 0.15
characteristics.characteristic: optical density
characteristics.text: Nicotiana benthamiana
characteristics.characteristic: organism
characteristics.text: Nicotiana benthamiana
characteristics.characteristic: organism
characteristics.text: leaf component
characteristics.characteristic: organism part
characteristics.text: Nicotiana benthamiana
characteristics.characteristic: scientific_name
characteristics.text: ub
characteristics.characteristic: target site
characteristics.text: _015_TRVWT_2_ub_2
characteristics.characteristic: title