characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2021-12-13T16:28:51Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2021-12-13T16:28:51Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10418838
characteristics.characteristic: External Id
characteristics.text: Center for Molecular Biology of Heidelberg University (ZMBH)
characteristics.characteristic: INSDC center name
characteristics.text: 2021-12-13T16:28:51Z
characteristics.characteristic: INSDC first public
characteristics.text: 2021-12-13T16:28:51Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8071682
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11087:SN166
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: neural cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002319
characteristics.characteristic: cell type
characteristics.text: sea lamprey
characteristics.characteristic: common name
characteristics.text: Protocols: Tissue samples were dissected from wild caught specimens. Sampled animals were euthanized by submersion in 0.1% MS-222 (Sigma, A5040-25G), unless specified otherwise, followed by decapitation according to local guidelines. Cells were isolated through a combination of mechanical and chemical lysis. Per library 15 000 - 20 000 nuclei were loaded into one channel of the ChromiumTM Single Cell A Chip (10X Genomics®). cDNA amplification and scRNA-seq libraries were constructed with Single-Cell 3′ Gel Bead and Library v2 (for larvae) and v3 Kit (for adults), following the instructions of the manufacturer.
characteristics.characteristic: description
characteristics.text: adult, mature
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001272
characteristics.characteristic: developmental stage
characteristics.text: LAM Adult 1 060419
characteristics.characteristic: individual
characteristics.text: Petromyzon marinus
characteristics.characteristic: organism
characteristics.text: Petromyzon marinus
characteristics.characteristic: organism
characteristics.text: encephalon
characteristics.characteristic: organism part
characteristics.text: Petromyzon marinus
characteristics.characteristic: scientific_name
characteristics.text: not available
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001071
characteristics.characteristic: sex
characteristics.text: SN166
characteristics.characteristic: title