characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-01-20T12:53:13Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-01-20T12:53:13Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10218767
characteristics.characteristic: External Id
characteristics.text: Institute of Life Sciences, Nalco Square, Chandrasekharpur, Bhubaneswar, Odisha
characteristics.characteristic: INSDC center name
characteristics.text: 2022-01-20T12:53:13Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-01-20T12:53:13Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7875127
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10994:Sample 4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HL-60
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002793
characteristics.characteristic: cell line
characteristics.text: lymphoblast
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000572
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: HL-60 cells were procured from ATCC and maintained in IMDM (GIBCO), supplemented with 20 % FBS (GIBCO). Total RNA was extracted using RNA isolation kit (Zymoresearch) following manufacturer’s protocol. Quality and concentration was checked using a spectrophotometer (Multiskan GO, Thermo Scientific). Quality of RNA was assessed by NanoDrop™ (Thermo Scientific) and Tapestation (Aligent Technologies). RNA was quantified in a Qubit 3 Fluorimeter, using Qubit RNA HS Assay Kit 9thermo Scientific. 800 ng of total RNA with RIN values ≥ 8 were selected for library preparation using TruSeq™ stranded mRNA kit (Illumina) following manufacturer’s instructions.
characteristics.characteristic: description
characteristics.text: acute promyelocytic leukemia
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000224
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: blood
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000178
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 4
characteristics.characteristic: title