characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2021-12-31T00:11:39Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2021-12-31T00:11:39Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10107523
characteristics.characteristic: External Id
characteristics.text: Instituto Gulbenkian de Ciencia
characteristics.characteristic: INSDC center name
characteristics.text: 2021-12-31T00:11:39Z
characteristics.characteristic: INSDC first public
characteristics.text: 2021-12-31T00:11:39Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7764080
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10939:Cre
characteristics.characteristic: Submitter Id
characteristics.text: 15
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: blood cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000081
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Blood was collected from G6pC1 fl/fl or G6pc1 AlbΔ/Δ mice at the peak of infection (i.e. day 7) with a transgenic Pcc AS expressing GFP. Samples were diluted 1:7.5 in 1xPBS and iRBCs were sorted according FSC, SSC and GFP on a BD FACS Aria Ilu. Cells were washed twice with 1x PBS, counted, and adjusted to a concentration of 1200 cells/μL. Cell suspensions were processed with Chromium™ Single Cell Controller to generate barcoded single-cell gel bead emulsions (GEMs) following the Chromium Next GEM Single Cell 3ʹ protocol. The single-cell cDNA libraries were obtained after the GEM-RT (reverse- transcriptase) clean-up and cDNA amplification on a BioRad C1000 Touch™. Thermal Cycler with the following PCR thermal program: 1) 98 ºC for 3 min, 2) 98 ºC for 15 sec, 3) 63 ºC for 20 sec, 4) 72 ºC for 1 min, (Steps 2 to 4 cycled 11 x), 5) 72 ºC for 1 min and 6) 4 ºC Hold. The cDNA profiles were checked on a Fragment Analyzer System (Agilent Technologies) according to the HS NGS Fragment kit (Agilent Technologies) manual. The scRNA-seq libraries were generated from 10μl (25%) of the single-cell cDNA sample and indexed with the Chromium i7 Multiplex Kit (10x Genomics) on a BioRad C1000 Touch™ Thermal Cycler as follows: 1) 98 ºC for 45 sec, 2) 98 ºC for 20 sec, 3) 54 ºC for 30 sec, 4) 72 ºC for 20 sec, (Steps 2 to 4 cycled 15 x), 5) 72 ºC for 1 min and 6) 4 ºC Hold. The final 3’ Gene Expression libraries were verified and quanti fied on a TapeStation 4200 using the D1000 ScreenTape kit.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Sa CreERT2/Wt G6pc1 Δ/Δ
characteristics.characteristic: genotype
characteristics.text: GFP-positive
characteristics.characteristic: immunophenotype
characteristics.text: Plasmodium chabaudi chabaudi AS expressing GFP
characteristics.characteristic: infect
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: Cre
characteristics.characteristic: title