characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-02-28T00:20:13Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-02-28T00:20:13Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11312956
characteristics.characteristic: External Id
characteristics.text: School of Bioscience and Biotechnology
characteristics.characteristic: INSDC center name
characteristics.text: 2022-02-28T00:20:13Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-02-28T00:20:13Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8957810
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11200:B. pseudomallei in SEM 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: This study compared the expression profiles of B. pseudomallei from infected mice to B. pseudomallei cultivated in soil extract media. B. pseudomallei in vivo was created by infecting BALB/c strain mice spleens through the intraperitoneal route and harvesting the spleens on day 5 post infection. Total RNA was obtained from two different sources: 1) mouse organs and 2) bacterial cultures. For the mouse samples, the spleens of infected and non-infected mice were harvested and RNA extracted on day 5 after infection. Bacteria samples were grown in soil extract medium and harvested during the mid-log phase. Total RNA was extracted from mice spleen and cultivated bacteria using the Trizol method, followed by DNA contamination removal and RNA purification using the Qiagen RNAeasy kit. The TruSeq RNA Library Prep Kit was used to prepare an RNAseq sequence library.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: soil extract media
characteristics.characteristic: growth condition
characteristics.text: Burkholderia pseudomallei
characteristics.characteristic: organism
characteristics.text: Burkholderia pseudomallei
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organism part
characteristics.text: Burkholderia pseudomallei
characteristics.characteristic: scientific_name
characteristics.text: UKMD286
characteristics.characteristic: strain
characteristics.text: B. pseudomallei in SEM 1
characteristics.characteristic: title