characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-02-10T00:15:33Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-02-10T00:15:33Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11549095
characteristics.characteristic: External Id
characteristics.text: Crick Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2022-02-10T00:15:33Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-02-10T00:15:33Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS9195254
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11244:SHSY5Y_DOX_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: SH-SY5Y
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002717
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: SH-SY5Y cells were transduced with SmartVector lentivirus (V3IHSHEG_6494503) containing a doxycycline-inducible shRNA cassette for TDP-43. Transduced cells were selected with puromycin (1 μg/mL) for one week. RNA was extracted with Trizol and column cleanup. RNA was extracted with Trizol and column cleanup Following reverse transcription with specific primers, nested PCR (keeping cycle number low) was used to obtain pure amplicons with Nanopore-compatible overhangs. Nanopore barcodes were then added and Nanopore adapters were added via rapid adapter addition.
characteristics.characteristic: description
characteristics.text: neuroblastoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000621
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: SHSY5Y_DOX_2
characteristics.characteristic: title