characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-02-28T00:20:42Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-02-28T00:20:42Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA12562154
characteristics.characteristic: External Id
characteristics.text: University of Salerno
characteristics.characteristic: INSDC center name
characteristics.text: 2022-02-28T00:20:42Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-02-28T00:20:42Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10168686
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11354:Sample 21
characteristics.characteristic: Submitter Id
characteristics.text: 82
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: clonal hematopoiesis of indeterminate potential
characteristics.characteristic: clinical information
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Aortic valve leaflets removed during surgery were immediately immersed in RNAlater (ThermoFisher Scientific, Waltham, Massachusetts, USA). A cusp of aortic valves removed during surgery was immediately fixed in 10% neutral buffered formalin for 24h and then embedded in paraffin for histologic evaluations. Aortic valves leaflets preserved in RNAlater (ThermoFisher Scientific, Waltham, Massachusetts, USA) were disrupted and homogenized with the TissueRuptor® (Qiagen, Hilden, Germany). Total RNA was extracted with RNeasy® Fibrous Tissue Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. Indexed libraries were prepared starting from 150 ng of purified RNA with TruSeq Stranded Total RNA Library Prep Kit (Illumina) according to the manufacturers instructions. Libraries size distribution was assessed using the Agilent 2200 TapeStation System (Agilent Technologies) and pooled in equimolar amounts, with a final concentration of 1,5 nM.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: aortic valve calcification; aortic stenosis
characteristics.characteristic: disease
characteristics.text: ID111
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: aortic valve
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002137
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 21
characteristics.characteristic: title