characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-01-27T16:18:42Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-01-27T16:18:42Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA12361846
characteristics.characteristic: External Id
characteristics.text: EMBL-EBI
characteristics.characteristic: INSDC center name
characteristics.text: 2022-01-27T16:18:42Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-01-27T16:18:42Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS9968631
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11267:WSSS_F_LNG10282022
characteristics.characteristic: Submitter Id
characteristics.text: 8
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Protocol carried out by HDBR to extract specimen from a fetal donor. Human fetal lung organoids were derived and maintained as previously described (Nikolic et al., 2017). In brief, human foetal lung tissues were treated with Dispase (8 U/ml Thermo Fisher Scientific, 17105041) at room temperature (RT) for 2 min to digest mesenchymal connections. Mesothelium and mesenchymal cells were carefully removed by needles. Branching epithelial tips were micro-dissected by needles, transferred into Matrigel (356231, Corning) and seeded in a 24 well low-attachment plate (M9312-100EA, Greiner) with 4-5 tips per 50 μl Matrigel dome per well. The plate was incubated at 37°C for 5-10 min until the Matrigel domes solidified. 600 μl of self-renewing differentiation medium was added. Organoids were cultured under standard tissue culture conditions (37°C, 5% CO2), maintained in self-renewing medium and passaged by mechanically breaking using P200 pipettes every 4-7 days. Organoids were then fully dissociated into single cells by incubation with prewarmed TrypLE (Thermo Fisher Scientific, 12605028) for 15-20 min with trituration every 5 min. Libraries were prepared according to the standard protocol of the Chromium 5'V1 kit.
characteristics.characteristic: description
characteristics.text: Carnegie stage 22
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/HsapDv_0000029
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: ASCL1 overexpression
characteristics.characteristic: genotype
characteristics.text: organoid culture
characteristics.characteristic: growth condition
characteristics.text: HDBR1915||HDBR2174||HDBR-N13393
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: lung
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002048
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: WSSS_F_LNG10282022
characteristics.characteristic: title