characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-02-28T12:35:28Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-02-28T12:35:28Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA12311253
characteristics.characteristic: External Id
characteristics.text: HENAN AGRICULTURAL UNIVERSITY
characteristics.characteristic: INSDC center name
characteristics.text: 2022-02-28T12:35:28Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-02-28T12:35:28Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS9918099
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11331:Sample 10
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: inbred line
characteristics.characteristic: cultivar
characteristics.text: Protocols: Maize leaves were collected at 3rd leaf stage after by 0.3 M mannitol treatment of roots for 4 h for RNA extraction. Maize seedlings were grown in Hogland’s nutrient solution at 400 mol m-2 s-1 photosynthetically active radiation, a 14/10-h day/night cycle and a day/night temperature of 28oC/22oC (relative humidity of 75%). When the second leaves were fully expanded, seedlings were transferred in 0.3 M mannitol in Hogland’s solution for 4 h and then sampled for RNA extraction. CTAB PBIOZOL and ethanol precipitation protocol was used for extraction and purification of total RNA from maize leaf tissue. mRNA was fragmented and enriched with by oligo-dT beads magnetic beads. Sequencing library was amplified with the broken mRNA as the templates to synthesize cDNA. The cDNA templates were purified with the kit and enriched by PCR.
characteristics.characteristic: description
characteristics.text: 3rd-leaf stage
characteristics.characteristic: developmental stage
characteristics.text: Chang7-2t
characteristics.characteristic: genotype
characteristics.text: Zea mays
characteristics.characteristic: organism
characteristics.text: Zea mays
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4577
characteristics.characteristic: organism
characteristics.text: leaf lamina
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0020039
characteristics.characteristic: organism part
characteristics.text: 1st leaf
characteristics.characteristic: sampling site
characteristics.text: Zea mays
characteristics.characteristic: scientific_name
characteristics.text: 0.3 M mannitol treated seedlings
characteristics.characteristic: stimulus
characteristics.text: Sample 10
characteristics.characteristic: title