characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-02-21T12:30:29Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-02-21T12:30:29Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11305746
characteristics.characteristic: External Id
characteristics.text: Cancer Sciences Faculty of Medicine University of Southampton
characteristics.characteristic: INSDC center name
characteristics.text: 2022-02-21T12:30:29Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-02-21T12:30:29Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8952533
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11195:1384_24H_DMSO_aIgM
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: IGHV mutated M-CLL
characteristics.characteristic: clinical information
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Samples were collected from patients following informed consent and in accordance with Ethics Committee approvals and the Declaration of Helsinki. Heparinized peripheral blood mononuclear cells (PBMCS) were obtained from patients attending clinic at the Southampton General Hospital. Cryopreserved samples were recovered and rested for one hour in complete RPMI-1640 (RPMI-1640 medium supplemented with 10% fetal bovine serum, 2 mM L-glutamine and 1% penicillin/streptomycin) at 37 °C before use. Cell viability was determined by trypan blue exclusion and was ≥90% in all cases. Cells were incubated with goat F(ab’)2 anti-human anti-IgM-coated or control antibody-coated DynaBeads at 37°C for 6 or 24 hours. Beads were added at a 2 beads per cell ratio. DMSO was added to all conditions as a vehicle control as part of a larger experiment. Promega Reliaprep RNA Cell Miniprep System Illumina TruSeq Library Prep kit V2
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: chronic lymphocytic leukemia
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000095
characteristics.characteristic: disease
characteristics.text: IGHV mutated
characteristics.characteristic: genotype
characteristics.text: 1384
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: blood
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000178
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: anti-IgM antibody treatment
characteristics.characteristic: stimulus
characteristics.text: 1384_24H_DMSO_aIgM
characteristics.characteristic: title