characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-01-24T16:27:32Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-01-24T16:27:32Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10418782
characteristics.characteristic: External Id
characteristics.text: Cambridge Institute of Therapeutic Immunology & Infectious Disease
characteristics.characteristic: INSDC center name
characteristics.text: 2022-01-24T16:27:32Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-01-24T16:27:32Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8071626
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11086:Oxy_2
characteristics.characteristic: Submitter Id
characteristics.text: 5
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: zebrafish
characteristics.characteristic: common name
characteristics.text: Protocols: Zebrafish embryos were exposed to a drug from 1 dpf to 5 dpf and at the end of the exposure period, larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 μl RLT buffer (Qiagen) containing 1.1 μl of 14.3M β-mercaptoethanol (Sigma). The lysate was allowed to bind to 450 μl of Agencourt AMPure XP beads (Beckman Coulter) for 15 minutes. The tubes were left on a magnet (Invitrogen) until the solutions cleared and the supernatant was removed without disturbing the beads. Whilst still on the magnet, beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer’s instructions and treated with DNase I (NEB, Catalogue number M0303L). Illumina TruSeq Stranded mRNA Sample Prep Kit.
characteristics.characteristic: description
characteristics.text: larval day 5
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001305
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: pool of 7 embryos
characteristics.characteristic: individual
characteristics.text: Danio rerio
characteristics.characteristic: organism
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organism part
characteristics.text: 2
characteristics.characteristic: replicate
characteristics.text: Danio rerio
characteristics.characteristic: scientific_name
characteristics.text: not available
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001071
characteristics.characteristic: sex
characteristics.text: Tüpfel long fin (TLF)
characteristics.characteristic: strain
characteristics.text: Oxy_2
characteristics.characteristic: title