characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-03-01T20:41:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-03-01T20:41:36Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10289565
characteristics.characteristic: External Id
characteristics.text: PhD student, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-01T20:41:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-03-01T20:41:36Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7944369
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11025:Sample 4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: B16-F10-Luc2
characteristics.characteristic: cell line
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: B16F10-luc2 melanoma cells were cultured in DMEM with pyruvate and 10% FBS. Extracellular vesicles were isolated from cell culture supernatant by size exclusion chromatography using iZON qEV columns according to the manufacturer's recommendation. For a complete protocol, please refer to Leary N, Walser S, Dieterich L.C., Methods Mol Biol (in press). Whole RNA from cells and EVs was extracted using the Macherey Nagel Nucleospin kit according to the manufacturer's instructions. Libraries were prepared using a miniaturised version of the Smart-seq2 protocol (Picelli et al., 2014).
characteristics.characteristic: description
characteristics.text: melanoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000756
characteristics.characteristic: disease
characteristics.text: extracellular vesicles
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/GO_0070062
characteristics.characteristic: fraction
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: skin
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: C57Bl/6N
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004472
characteristics.characteristic: strain
characteristics.text: Sample 4
characteristics.characteristic: title