characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-02-09T00:32:23Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-02-09T00:32:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10159115
characteristics.characteristic: External Id
characteristics.text: INSERM Gustave Roussy institute
characteristics.characteristic: INSDC center name
characteristics.text: 2022-02-09T00:32:23Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-02-09T00:32:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7815601
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10924:9_23_2_transloque_S9
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: T cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000084
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: To recapitulate the t(2;5)(p23;q35) chromosomal translocation, activated T lymphocytes from healthy donors PBMC were transfected with the RNP complex composed of the protein Cas9 with gRNA NPM1 (targeting NPM1 gene) and gRNA ALK (targeting ALK gene). Control cells were wild-type CD4+ activated lymphocytes. Cells were grown for 10-15 days in vitro prior RNA extraction or engraftment in vivo. Mice engrafted with NPM1-ALK+ T-cells developed T-cell lymphoma after 2-5 months. Human lymphoma cells were purified based on human CD45+CD3- or CD45+CD3+ expression by flow cytometry prior to RNA extraction RNA was extracted using RNeasy mini Kit (Qiagen) SureSelect Strand-Specific RNA Library Prep System Poly-A Selection and Strand-Specific mRNA Library Preparation for the Illumina Platform
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: CRISPR Cas9 induced fusion of NPM1 and ALK
characteristics.characteristic: genetic modification
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: in vitro cultured
characteristics.characteristic: growth condition
characteristics.text: CD4 positive
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000084
characteristics.characteristic: immunophenotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: blood
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000178
characteristics.characteristic: organism part
characteristics.text: anaplastic large cell lymphoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0003032
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: 9_23_2_transloque_S9
characteristics.characteristic: title