characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-01-24T16:27:46Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-01-24T16:27:46Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11514650
characteristics.characteristic: External Id
characteristics.text: Department of General Surgery, The Third Affiliated Hospital of Southern Medical University
characteristics.characteristic: INSDC center name
characteristics.text: 2022-01-24T16:27:46Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-01-24T16:27:46Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS9160855
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11257:SK-control3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: SK-N-SH
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0003072
characteristics.characteristic: cell line
characteristics.text: neuroblast (sensu Vertebrata)
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000031
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Cell lines used in our study were purchased from Procell Life Science & Technology (Wuhan, China), and were cultured in standard medium containing 10% fetal bovine serum. Cell lines authentication were performed using short tandem repeat DNA profiling, and mycoplasma detection was regularly performed using a PCR-based method. All cell lines were cultured in a humidified atmosphere with 5% CO2 at 37°C Total RNA was isolated using the Trizol reagent (Invitrogen), and conversion to cDNA was completed using the GoScript Reverse Transcription System (Promega). The relevant mRNA expression of target genes was quantified by Real-time PCR using a SYBR Green PCR Kit (Invitrogen) normalized to GAPDH. Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to hg19 whole genome using Tophat2 v2.0.13 with parameters --read_mismatches 2 --read_gap_length 2 --read_edit_dist 2
characteristics.characteristic: description
characteristics.text: neuroblastoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000621
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: SK-control3
characteristics.characteristic: title