characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-01-26T12:42:40Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-01-26T12:42:40Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA12564657
characteristics.characteristic: External Id
characteristics.text: Plant Sciences, School of Life Sciences, University of Dundee
characteristics.characteristic: INSDC center name
characteristics.text: 2022-01-26T12:42:40Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-01-26T12:42:40Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10171185
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11358:CEM01_JH15-35
characteristics.characteristic: Submitter Id
characteristics.text: 5
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HEB15_124
characteristics.characteristic: cultivar
characteristics.text: Protocols: At early stem elongation plants were uprooted from their pots and roots were washed from the adhering rhizosphere soil using vortexing and mild saline buffer prior nucleic acid extraction. Plants were grown in a randomized design in a glasshouse at 18/14 °C (day/night) temperature regime with 16 h daylight that was supplemented with artificial lighting to maintain a minimum light intensity of 200 µmol quanta m−2 s−1. RNA was extracted from individual root systems with the Macherey-Nagel™ NucleoSpin™ RNA, Mini Kit (Thermo Fisher, USA) following the manufacturer’s protocol, including the Plant RNA Isolation Aid Invitrogen (Thermo Fisher, USA) for the sample lysis step using 90 µL of Plant RNA Isolation Aid, mixed with 870 µL of RA1 buffer and 40 µL of dithiothreitol (DTT). RNA quality was assessed using an Agilent 2100 Bioanalyzer or TapeStation (Agilent, USA). Samples for sequencing were selected based on microbiota profiles and quality of the RNA sample Total RNA (300 ng) was further purified using the NEBNext mRNA Magnetic Isolation Module (NEB). Library preparation was carried out using the NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads and indexed with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs set 1).
characteristics.characteristic: description
characteristics.text: BBCH15 cereal growth stage
characteristics.characteristic: developmental stage
characteristics.text: 124_52
characteristics.characteristic: genotype
characteristics.text: green house study
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/EO_0007248
characteristics.characteristic: growth condition
characteristics.text: Hordeum vulgare
characteristics.characteristic: organism
characteristics.text: Hordeum vulgare
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4513
characteristics.characteristic: organism
characteristics.text: root structure
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000989
characteristics.characteristic: organism part
characteristics.text: Hordeum vulgare
characteristics.characteristic: scientific_name
characteristics.text: CEM01_JH15-35
characteristics.characteristic: title