characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-02-04T00:14:26Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-02-04T00:14:26Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA12562133
characteristics.characteristic: External Id
characteristics.text: Department of Pathology,the First Affiliated Hospital of Soochow University
characteristics.characteristic: INSDC center name
characteristics.text: 2022-02-04T00:14:26Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-02-04T00:14:26Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10168665
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11322:HCT8R_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HCT8
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002189
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: HCT116 and HCT8 cells were cultured within Dulbecco's Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS). HCT116 cells were long-term cultured within DMEM containing 10% FBS and 1 μg/mL oxaliplatin. HCT8 cells were long-term cultured within DMEM containing 10% FBS and 0.2 μg/mL oxaliplatin. 1 x 107 cells were stored in 1 mL TRIZOL (Thermo Fisher Scientific) and ground in liquid nitrogen. Next, 100 μL chloroform was added, and the cells were fully mixed and centrifuged at the highest speed at 4 °C for 10 min. The supernatant was transferred into a new tube, isopropanol was added to the same volume, and cells were centrifuged at the highest speed at 4 °C for 10 min. The precipitate was washed with 75% cold ethanol and dissolved in DEPC water. The concentration and quality of RNA were measured using a Nanodrop 2000 (Thermo Fisher Scientific) and an Agilent Bioanalyzer 2100 (Agilent, USA). A total of 5 μg of RNA from each group was used for library preparation using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, USA) following the manufacturer’s instructions
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: colorectal cancer
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005842
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: colorectum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0012652
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: HCT8R_2
characteristics.characteristic: title