characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-02-08T00:19:00Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-02-08T00:19:00Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA12119977
characteristics.characteristic: External Id
characteristics.text: IP
characteristics.characteristic: INSDC center name
characteristics.text: 2022-02-08T00:19:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-02-08T00:19:00Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS9766610
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11233:4544
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: isolated from nasal swab
characteristics.characteristic: clinical information
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Nasal swab from patients with symptoms of SARS-CoV-2 RNA extraction and qRT-PCR were carried out by the French National Center for Respiratory infection virus (32156327). double-stranded cDNA was synthetized using random hexamers and the ProtoScript II first strand cDNA synthesis kit followed by the NEBNext Ultra II Non Directional RNA Second Strand Synthesis Module from New England Biolabs. Indexed library preparation was made using the Nextera Flex for enrichment (Illumina) kit following the manufacturer’s protocol without modification. Indexed libraries were pooled according to the results of the RT-qPCR CT for SARS-CoV-2 of each sample. Illumina hybridization reactions were made using the Nextera Flex enrichment protocol as recommended by the manufacturer
characteristics.characteristic: description
characteristics.text: Severe acute respiratory syndrome coronavirus 2
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_2697049
characteristics.characteristic: infect
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: nasal cavity
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001707
characteristics.characteristic: organism part
characteristics.text: Illumina respiratory virus hybridisation
characteristics.characteristic: protocol
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 4544
characteristics.characteristic: title
characteristics.text: CT36+
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006982
characteristics.characteristic: viral load