characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-02-28T00:30:53Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-02-28T00:30:53Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11549301
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY COLLEGE LONDON
characteristics.characteristic: INSDC center name
characteristics.text: 2022-02-28T00:30:53Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-02-28T00:30:53Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS9195460
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10237_2:Patient 4
characteristics.characteristic: Submitter Id
characteristics.text: 74
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: RNA was isolated from temporal cortex tissue of 10 FTLD brains. 50 mg of flash-frozen tissue was homogenised in 700 µl of Qiazol (Qiagen) using a TissueRuptor II (Qiagen). Chloroform was added and RNA subsequently extracted following the spin-column protocol from the miRNeasy kit with DNase digestion (Qiagen). RNA was eluted off the column in 50 µl of RNAse-free water. Purified RNA was reverse transcribed with Superscript IV (Thermo Fisher Scientific) using either sequence specific primers containing sample-specific barcodes or random hexamers, following the manufacturer recommendations. Unique molecular identifiers (UMIs) and part of the P5 Illumina sequence were added either during first- or second-strand-synthesis (with Phusion HF 2x Master Mix) respectively. Three rounds of nested PCR using Phusion HF 2x Master Mix (New England Biolabs) were used to obtain highly specific amplicons for the UNC13A cryptic, followed by gel extraction and a final round of PCR in which the full length P3/P5 Illumina sequences were added.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: FTLD-TDPC
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: temporal cortex
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0016538
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Patient 4
characteristics.characteristic: title