characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-02-04T00:14:26Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-02-04T00:14:26Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA12561434
characteristics.characteristic: External Id
characteristics.text: Department of Pathology,the First Affiliated Hospital of Soochow University
characteristics.characteristic: INSDC center name
characteristics.text: 2022-02-04T00:14:26Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-02-04T00:14:26Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10167966
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11323:P2
characteristics.characteristic: Submitter Id
characteristics.text: 64
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Human CRC tissues were harvested by surgery. Tissues containing 1 x 106 cells were stored in 1 mL TRIZOL (Thermo Fisher Scientific) and ground in liquid nitrogen. Next, 100 μL chloroform was added, and the cells were fully mixed and centrifuged at the highest speed at 4 °C for 10 min. The supernatant was transferred into a new tube, isopropanol was added to the same volume, and cells were centrifuged at the highest speed at 4 °C for 10 min. The precipitate was washed with 75% cold ethanol and dissolved in DEPC water. The concentration and quality of RNA were measured using a Nanodrop 2000 (Thermo Fisher Scientific) and an Agilent Bioanalyzer 2100 (Agilent, USA). A total of 2 μg of RNA from each group was used for library preparation using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, USA) following the manufacturer’s instructions
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: colorectal cancer
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005842
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: colon
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: P2
characteristics.characteristic: title