characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-02-28T00:20:42Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-02-28T00:20:42Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA12562262
characteristics.characteristic: External Id
characteristics.text: University of Salerno
characteristics.characteristic: INSDC center name
characteristics.text: 2022-02-28T00:20:42Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-02-28T00:20:42Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10168793
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11352:Sample 88
characteristics.characteristic: Submitter Id
characteristics.text: 75
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: no clonal hematopoiesis of indeterminate potential
characteristics.characteristic: clinical information
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Blood samplings were performed from an antecubital vein using a 21-gauge needle or from the central venous line. Blood was collected in the early morning at least 12 hours after the last administration of anticoagulant drugs. The first 2 to 4 mL of blood were discarded, and the remaining was collected in EDTA tubes aliquoted and immediately stored at -80 °C. DNA was extracted from whole blood samples with DNeasy Blood & Tissue Kit (Qiagen), according to the manufacturer's instructions. Libraries were prepared using SureSelectXT Low Input Target Enrichment System for Illumina Paired-End Multiplexed Sequencing Library protocol (Agilent technologies), starting from 200 ng of gDNA and according to manufacturers instructions. Hybridization capture of DNA libraries was performed through a custom RNA probes panel designed to enrich the 9 genes commonly mutated in CHIP. To improve the identification of low allele frequency variants and exclude PCR duplicates, 10-bp unique molecular identifiers (UMIs) were incorporated into each DNA fragment during library preparation. Libraries quality and size distribution were assessed on Agilent 2200 TapeStation System (Agilent Technologies) with the DNA1000 Kit.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: aortic valve calcification; aortic stenosis
characteristics.characteristic: disease
characteristics.text: ID91
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Sample 88
characteristics.characteristic: title