characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-01-31T00:14:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-01-31T00:14:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10838634
characteristics.characteristic: External Id
characteristics.text: UCD School of Biomolecular and Biomedical Science
characteristics.characteristic: INSDC center name
characteristics.text: 2022-01-31T00:14:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-01-31T00:14:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8489868
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11138:GSKTGFB
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: wtsli028-a
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: iPSCs were differentiated into kidney organoids over 24days using an adapted protocol from Takasato et al. 2016. Organoids were pre treated on day 24 with 5uM GSK343 for one hour prior to treatment with 10ng/ml human recombinant TGFB1 for 24h followed by another treatment after 24h. On day 26, 2 organoids were pooled and dissociated into single cells using a cold active protease. Single cell suspensions were loaded onto 10X Genomics Single Cell 3' Chip G (Cat number: 1000127) along with the reverse transcription (RT) master mix as per the manufacturer's protocol to generate single cell GEMs (Gel beads in emulsion) for approximately 5000 cells per sample. Libraries were prepared using the Chromium Single Cell 3' v3 Library and Gel Bead Kit (Cat number: 1000128) and Single Index Kit T Set A (Cat number: 1000213) as per manufacturers (10X Genomics) instructions.
characteristics.characteristic: description
characteristics.text: kidney organoid culture
characteristics.characteristic: growth condition
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: induced pluripotent stem cell
characteristics.characteristic: progenitor cell type
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: GSKTGFB
characteristics.characteristic: title