characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-01-31T00:14:07Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-01-31T00:14:07Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11325798
characteristics.characteristic: External Id
characteristics.text: Maastricht University
characteristics.characteristic: INSDC center name
characteristics.text: 2022-01-31T00:14:07Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-01-31T00:14:07Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8971029
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11221:Sample 29
characteristics.characteristic: Submitter Id
characteristics.text: 67
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Remove medium from each well and add 200 µL of QIazol Lysis reagent (Qiagen, The Netherlands) into the wells to dissociate the matrigel matrix and transfer each sample to the respective tubes. Repeat this step 2 or 3 times until all organoids are collected to a total volume of QIazol Lysis reagent of 700 µL. Performed using miRNeasy Mini Kit (Qiagen, The Netherlands), following manufacturer\u2019s protocol for Animal Cells including a DNase treatment. Samples with purified RNA were prepared for sequencing using Lexogen SENSE mRNA- seq protocol and library preparation kit (Lexogen, The Netherlands).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: organoid culture
characteristics.characteristic: growth condition
characteristics.text: 1
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: colon
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001155
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Sample 29
characteristics.characteristic: title