characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-03-22T20:40:44Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-03-22T20:40:44Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10558493
characteristics.characteristic: External Id
characteristics.text: VIB-Ugent center for plant systems biology
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-22T20:40:44Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-03-22T20:40:44Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8210474
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11108:Section1_replica5
characteristics.characteristic: Submitter Id
characteristics.text: 2 days after leaf 4 appearance
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: B104
characteristics.characteristic: cultivar
characteristics.text: Protocols: Three zones were sampled, from the base to 3.5 cm, from 3.5 to 7.0 cm and from 7.0 to 10.5 cm, of the developing fourth leaf of maize B104 plants at two days after leaf appearance (DALA). Only leaf samples were taken from well-watered plants. To sample the three zones of a developing fourth leaf two days after leaf emergence, the older leaves that surround the growing leaf should be removed. Special care should be taken not to damage the basal part of the growing leaf because this is where the leaf basal meristem is located. Subsequently the fourth leaf was cut into three parts corresponding to the three sampled zones. B104 seeds were sown in soil and grown in a controlled-environment growth chamber (relative humidity (55%), temperature (24 °C day/18 °C night) and 16 h/8 h (day/night) cycle). Only well-watered plants. Purified poly(A)-containing mRNA molecules were fragmented, randomly primed strand-specific cDNA was generated and adapters were ligated. Total RNA was extracted using the guanidinium thiocyanate-phenol-chloroform extraction method using TRI-reagent (Thermo Fisher Scientific) followed by DNA digestion using the RQ1 RNase-free DNase kit (Promega). Library preparation was done using the NEBNext Kit (Illumina).
characteristics.characteristic: description
characteristics.text: LP.04 four leaves visible stage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007115
characteristics.characteristic: developmental stage
characteristics.text: well watered
characteristics.characteristic: environmental history
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: growth chamber study
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/EO_0007269
characteristics.characteristic: growth condition
characteristics.text: Zea mays
characteristics.characteristic: organism
characteristics.text: Zea mays
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4577
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: organism part
characteristics.text: leaf 4, cm 0 - 3.5 measured from the base of the leaf. Division and expansion zone.
characteristics.characteristic: sampling site
characteristics.text: Zea mays
characteristics.characteristic: scientific_name
characteristics.text: Section1_replica5
characteristics.characteristic: title