characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-03-15T00:16:16Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-03-15T00:16:16Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13245067
characteristics.characteristic: External Id
characteristics.text: Lund University Department of Experimental Medical Sciences Lung Bioengineering and Regeneration
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-15T00:16:16Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-03-15T00:16:16Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10848146
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11502:Proximal_ALI_p1_2
characteristics.characteristic: Submitter Id
characteristics.text: 8 to 12
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: lung epithelium
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000115
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: 3DLD isolation method was used to isolate both proximal and distal progenitors from individual mice. Proximal epithelial cells were expanded to passage 1 and collected by pelleting the cells. Distal progenitors from 3DLD isolation or Classic isolation were directly pelleted after isolation protocol. Qiagen RNeasy micro kit (74004, QIAGEN, Sweden) was used to isolate total RNA following the manufacturer’s protocol. RNA concentrations were measured using NanoDrop 1000 and Qubit™ RNA HS Assay Kit (Q32852, Fisher Scientific). RNA quality was evaluated by the agilent 2100 BioAnalyzer. Library preparation was done using the Illumina Stranded mRNA Prep Ligation (20040534, Illumina) with 14 cycles under final PCR amplification and samples were indexed with IDT for Illumina RNA UD Indexes Set B, Ligation (20040554, Illumina) for multiplexing. Clean up steps were automated using the King Fisher FLEX (18-5400620, Thermo Scientific). Library concentration was checked using the QuantIT 1X dsDNA HS Assay Kit (Q33232) and library size and quality were checked using the 5200 Fragment Analyzer™ 12-capillary (M3510AA, Agilent) using the DNF-930 dsDNA Reagent Kit, 75 bp - 20,000 bp(500 Samples) (Part # DNF-930-K0500, Agilent).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: proximal epithelial differentiation medium
characteristics.characteristic: growth condition
characteristics.text: m2p1
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: trachea
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0003126
characteristics.characteristic: organism part
characteristics.text: basal cell of epithelium of trachea
characteristics.characteristic: progenitor cell type
characteristics.text: 3DLD proximal isolation
characteristics.characteristic: protocol
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: C57BL/6N
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004472
characteristics.characteristic: strain
characteristics.text: Proximal_ALI_p1_2
characteristics.characteristic: title