characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-03-05T00:14:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-03-05T00:14:08Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13151277
characteristics.characteristic: External Id
characteristics.text: Laboratory for Experimental Orthopedics, Maastricht UMC+
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-05T00:14:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-03-05T00:14:08Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10754884
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11469:TWE34
characteristics.characteristic: Submitter Id
characteristics.text: 16
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: chondrocyte
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000138
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Non-osteoarthritic human articular chondrocytes were plated at 30 000 cells/cm2 and exposed to 20% (v/v) OA-SF (pool of 14 patients, equal volume ratios), or 20% (v/v) 0.9% NaCl for 14 days. The experiment was performed in biological replicates (5 donors). The medium was refreshed every other day. Cells were cultured in DMEM/F-12 low glucose with GlutaMAX (Thermo Fisher Scientific, 31331-028) supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich, F7524), 1% A-A (Thermo Fisher Scientific, 15240-062), and 1% nonessential amino acids (Thermo Fisher Scientific, 11140-035) at 37°C in a humidified atmosphere containing 5% CO2. Cells were exposed to 20% (v/v) OA-SF (pool of 14 patients, equal volume ratios), or 20% (v/v) 0.9% NaCl for 14 days. Cells were exposed to 20% (v/v) OA-SF (pool of 14 patients, equal volume ratios), or 20% (v/v) 0.9% NaCl for 14 days. Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen,74134) following the manufacturer's protocol. RNA quality was determined with a 2100 Bioanalyzer (Agilent) and RNA 6000 Nano Kit (Agilent, 5067-1511). All samples had RNA integrity (RIN) number greater than 9, which was considered sufficient for further analysis. Total RNA was randomly fragmented by alkaline hydrolysis, RNA fragments were end-repaired and converted to library using NEBNext Small RNA Library kit.
characteristics.characteristic: description
characteristics.text: osteoarthritic synovial fluid
characteristics.characteristic: growth condition
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: articular bone
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0003820
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: TWE34
characteristics.characteristic: title