characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-03-03T20:47:53Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-03-03T20:47:53Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13207859
characteristics.characteristic: External Id
characteristics.text: Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Universite Paris-Saclay, 78000 Versailles, France
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-03T20:47:53Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-03-03T20:47:53Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10810634
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11497:CRIS2#2
characteristics.characteristic: Submitter Id
characteristics.text: 7
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Switzerland Gransden
characteristics.characteristic: cultivar
characteristics.text: Protocols: Protonema were grown for 7days on PpNH4 medium (F. Charlot et Al, Physcomitrium patens protoplasting and protoplast transfection, in: K. Wang, F. Zhang (Eds.), Methods Mol. Biol. (Clifton, N.J.)-Protoplast Technol., Springer Nature, 2022: p. In Press). Plants were cultured as described previously in (Cove DJ et al, 2009) Plants were transformed with Crispr-Cas9 as previously described in (Collonnier C. et al, 2017) Genomics DNA was extracted from 1.5 g of fresh protonema. DNA samples were extracted using the NucleoSpin™ PlantII Kit (Macherey-Nagel) as described by the manufacturer. Kapa Hyper Prep (index 12 bases)
characteristics.characteristic: description
characteristics.text: protonema
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0030003
characteristics.characteristic: developmental stage
characteristics.text: apt- 4
characteristics.characteristic: genotype
characteristics.text: Physcomitrium patens
characteristics.characteristic: organism
characteristics.text: Physcomitrium patens
characteristics.characteristic: organism
characteristics.text: protonema
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0030003
characteristics.characteristic: organism part
characteristics.text: Physcomitrium patens
characteristics.characteristic: scientific_name
characteristics.text: CRIS2#2
characteristics.characteristic: title