characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-03-03T20:47:53Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-03-03T20:47:53Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13207864
characteristics.characteristic: External Id
characteristics.text: Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Universite Paris-Saclay, 78000 Versailles, France
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-03T20:47:53Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-03-03T20:47:53Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10810639
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11497:TAL#2
characteristics.characteristic: Submitter Id
characteristics.text: 7
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Switzerland Gransden
characteristics.characteristic: cultivar
characteristics.text: Protocols: Protonema were grown for 7days on PpNH4 medium (F. Charlot et Al, Physcomitrium patens protoplasting and protoplast transfection, in: K. Wang, F. Zhang (Eds.), Methods Mol. Biol. (Clifton, N.J.)-Protoplast Technol., Springer Nature, 2022: p. In Press). Plants were cultured as described previously in (Cove DJ et al, 2009) Plants were transformed with TALEN as described in (Bessoltane N. et al, in submission). Genomics DNA was extracted from 1.5 g of fresh protonema. DNA samples were extracted using the NucleoSpin™ PlantII Kit (Macherey-Nagel) as described by the manufacturer. Kapa Hyper Prep (index 12 bases)
characteristics.characteristic: description
characteristics.text: protonema
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0030003
characteristics.characteristic: developmental stage
characteristics.text: apt- 6
characteristics.characteristic: genotype
characteristics.text: Physcomitrium patens
characteristics.characteristic: organism
characteristics.text: Physcomitrium patens
characteristics.characteristic: organism
characteristics.text: protonema
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0030003
characteristics.characteristic: organism part
characteristics.text: Physcomitrium patens
characteristics.characteristic: scientific_name
characteristics.text: TAL#2
characteristics.characteristic: title