characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-03-05T00:14:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-03-05T00:14:08Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13151284
characteristics.characteristic: External Id
characteristics.text: Laboratory for Experimental Orthopedics, Maastricht UMC+
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-05T00:14:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-03-05T00:14:08Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10754891
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11470:CRISPR Ctrl3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: SW1353
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002360
characteristics.characteristic: cell line
characteristics.text: fibroblast
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000057
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: SNORD71 KD and CRISPR control SW1353 cells were cultured in DMEM/F-12 low glucose with GlutaMAX (Thermo Fisher Scientific) supplemented with 10% FCS (Sigma-Aldrich), 1% A-A (Thermo Fisher Scientific) at 37°C in a humidified atmosphere and 5% CO2. Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen,74134) following the manufacturer's protocol. RNA quality was determined with a 2100 Bioanalyzer (Agilent) and RNA 6000 Nano Kit (Agilent, 5067-1511). All samples had RNA integrity (RIN) number greater than 9, which was considered sufficient for further analysis. Total RNA was randomly fragmented by alkaline hydrolysis, RNA fragments were end-repaired and converted to library using NEBNext Small RNA Library kit.
characteristics.characteristic: description
characteristics.text: bone chondrosarcoma
characteristics.characteristic: disease
characteristics.text: control
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001461
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: bone
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: CRISPR Ctrl3
characteristics.characteristic: title