characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-03-10T20:40:41Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-03-10T20:40:41Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10378438
characteristics.characteristic: External Id
characteristics.text: Department of Genetics and Evolution Federal University of Sao Carlos
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-10T20:40:41Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-03-10T20:40:41Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8033012
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11047:P42D180
characteristics.characteristic: Submitter Id
characteristics.text: 21
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: cured patient, 180 days after treatment
characteristics.characteristic: clinical history
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Peripheral blood samples from healthy donors, asymptomatic individuals, VL patients during active disease and cured VL patients (180 days after being treated) were collected using BD Vacutainer and PAXgene Blood RNA tubes. Patients diagnosed with visceral leishmaniasis (PD0 samples) were treated with AmBisome (liposomal amphotericin B) or Glucantime (meglumine antimoniate). After follow up of 180 days, patients that were considered clinically cured (PD180 samples) have composed the cured group. Total RNA samples were purified with the PAXgene Blood RNA Kit and GLOBINclear-Human kit. Libraries were prepared using a TruSeq Stranded RNA Sample Preparation kit (Illumina) with Poly(A)+ selection.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: visceral Leishmaniasis
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005045
characteristics.characteristic: disease
characteristics.text: cured
characteristics.characteristic: disease staging
characteristics.text: 20
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: blood
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000178
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: P42D180
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_85993
characteristics.characteristic: title