characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-03-09T00:13:56Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-03-09T00:13:56Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10113889
characteristics.characteristic: External Id
characteristics.text: Institute for Integrative Biology of the Cell (I2BC), Universite Paris-Saclay, CEA, CNRS, Universite Paris Sud
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-09T00:13:56Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-03-09T00:13:56Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7770443
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10951:FX489
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: β2163 cells harbouring the mini-TLC plasmids were collecting on LB plates supplemented with Dap (0.3mM) and chloramphenicol. genomic DNA extraction kit Plasmid attP sites were amplified by performing 17 PCR cycles on 50-100 ng of plasmid library gDNA with an equimolar mix of 4103-4105 P5 and 4178-4180/4246-4248 P7 primers. The products were purified from the P5 and P7 primers the double selection with AMPure.
characteristics.characteristic: description
characteristics.text: XafT+ ; tagn8
characteristics.characteristic: mini-TLC genotype
characteristics.text: Escherichia coli K-12
characteristics.characteristic: organism
characteristics.text: Escherichia coli K-12
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_83333
characteristics.characteristic: organism
characteristics.text: Escherichia coli K-12
characteristics.characteristic: scientific_name
characteristics.text: pir+ ; RP4 conjugation machinery+
characteristics.characteristic: strain genotype
characteristics.text: FX489
characteristics.characteristic: title