characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-03-11T00:18:37Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-03-11T00:18:37Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10774730
characteristics.characteristic: External Id
characteristics.text: Laboratory of Genomics and Bioinformatics Institute of Molecular Genetics of the Czech Academy of Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-11T00:18:37Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-03-11T00:18:37Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8425988
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11127:DHX38_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HEK293
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001182
characteristics.characteristic: cell line
characteristics.text: kidney cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_1000497
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: RNA was isolated directly from cultured HEK293 cells. HEK293 cells were cultured in high-glucose (4500 mg/l) DMEM medium (Sigma-Aldrich) supplemented with 10% foetal bovine serum, 1% penicillin and streptomycin. Cells were grown in 37 °C and 5% CO2 atmosphere. 50nM siRNA SMART Pool against DHX38 50nM was transfected into cells using Oligofectamine reagent (Invitrogen) at the final concentration of 50nM according to the manufacturer’s protocol. The negative control # 5 siRNA from Ambion was used as a negative control. Total RNA was isolated from harvested cells using TRIzol followed by Direct-zol RNA MicroPrep (Zymo Research). Libraries were prepared using KAPA RNA Hyperprep Kit with RiboErase (Kapa Biosystems/Roche).
characteristics.characteristic: description
characteristics.text: embryo stage
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0007725
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: kidney
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002113
characteristics.characteristic: organism part
characteristics.text: DHX38 knockdown
characteristics.characteristic: phenotype
characteristics.text: DHX38 siRNA
characteristics.characteristic: rna interference
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: DHX38_2
characteristics.characteristic: title