characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-03-03T00:15:51Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-03-03T00:15:51Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13093151
characteristics.characteristic: External Id
characteristics.text: Onderzoeksgroep Nefrologie en Niertransplantatie
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-03T00:15:51Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-03-03T00:15:51Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10697155
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11450:Sample 7
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: peripheral blood mononuclear cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_2000001
characteristics.characteristic: cell type
characteristics.text: stable kidney transplant
characteristics.characteristic: clinical information
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Peripheral blood sample collection: Blood samples were collected at time of indication biopsy or protocol biopsy at Nantes' hospital, France. They were isolated using density gradient and frozen in their respective serum complemented with 10% DMSO for storage in liquid nitrogen and shipment. Upon arrival in Leuven, Belgium, they were then thawed, centrifuged at 200g for 5 minutes at 4°C before resuspension in PBS containing 0.04% UltraPure BSA (AM2616, ThermoFisher Scientific) and strained through a 40 µm cell strainer to further remove cell clumps and large fragments. Cell number and viability was measured using Luna Cell counter. Libraries for scRNASeq were generated using the Chromium Single Cell 5′ library and Gel Bead & Multiplex Kit from 10x Genomics. We aimed to profile 5000 cells per library if sufficient cells were retained during the process. All libraries were sequenced on Illumina NextSeq until sufficient saturation was reached. A
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: 376 days post transplantation
characteristics.characteristic: time
characteristics.text: Sample 7
characteristics.characteristic: title