characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-03-12T00:16:39Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-03-12T00:16:39Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13207040
characteristics.characteristic: External Id
characteristics.text: Bone and Joint Surgery Center, Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-12T00:16:39Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-03-12T00:16:39Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10809816
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11493:Sample 5
characteristics.characteristic: Submitter Id
characteristics.text: 68
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Finally, subchondral bone samples from six patients who underwent primary total hip arthroplasty (three ONFH patients and three patients in control group with femoral neck fracture) were obtained. All the samples were snap-frozen and stored in liquid nitrogen until further analyses. Frozen subchondral bone samples were rapidly ground in liquid nitrogen. Total RNA was extracted by TRIzol Reagent (Invitrogen) following the manufacturer’s instructions. NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs), RiboZero Magnetic Gold Kit (Illumina), and KAPA Stranded RNA-Seq Library Prep Kit (Illumina) were used to achieve RNA enrichment and sequencing libraries generation.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: osteonecrosis
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004259
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: bone marrow
characteristics.characteristic: organism part
characteristics.text: femur
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000981
characteristics.characteristic: sampling site
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: Sample 5
characteristics.characteristic: title