characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-04-30T00:12:44Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-04-30T00:12:44Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10786597
characteristics.characteristic: External Id
characteristics.text: Section of Bioinformatics and Systems Cardiology, Klaus Tschira Institute for Integrative Computational Cardiology and Department of Internal Medicine III, University Hospital Heidelberg.
characteristics.characteristic: INSDC center name
characteristics.text: 2022-04-30T00:12:44Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-04-30T00:12:44Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8437836
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11124:Sample 7
characteristics.characteristic: Submitter Id
characteristics.text: 110
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Male mice were kept on a 129S6 background and analyzed at 100 to 120 days of age. Hearts were rapidly excised after cervical dislocation without previous anesthesia, washed in PBS, and dissected. The tissue was snap frozen in liquid nitrogen and stored at -80°C. Animals were kept at the Animal House of the Max-Delbrück Center for Molecular Medicine, Berlin. RNA from left ventricular tissue was isolated using TRIzol followed by a clean-up with the QIAGEN RNA micro kit. cDNA libraries were generated using the Illumina TruSeq Stranded mRNA Sample Prep Kit.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: PEVK homozygous knockout of exons 219-225; RBM20 heterozygous deletion of exons 6-7
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: heart left ventricle
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002084
characteristics.characteristic: organism part
characteristics.text: PEVK-KO-RBM-HET
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: 129S6
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000598
characteristics.characteristic: strain
characteristics.text: Sample 7
characteristics.characteristic: title