characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-04-26T16:19:20Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-04-26T16:19:20Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11308717
characteristics.characteristic: External Id
characteristics.text: Istituto Nazionale Genetica Molecolare (INGM)
characteristics.characteristic: INSDC center name
characteristics.text: 2022-04-26T16:19:20Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-04-26T16:19:20Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8953578
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11174:Donor1_MBC_2020
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: memory B cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000787
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Peripheral blood mononuclear cells (PBMC) from the year 2010 (Donor1) and year 2014 (Donor11) were isolated by the standard density-gradient centrifugation and cryopreserved until the day of use. Blood samples collected in 2020 from both Donor1 and Donor2 were processed immediately to isolate memory B cells and plasma cells by flow cytometry followed by single-cell immune profiling using the 10X system. Single cell suspension was performed according to Chromium Next GEM Single cell V(D)J Reagent kits v1.1. The positive fractions of memory B cells and plasma cells were collected in 6ul of FCS containing eppendorf tubes that were pre-coated overnight with 2percent BSA. The 5’scRNA-seq libraries were generated using Chromium Next GEM Single Cell V(D)J Reagent Kit v1.1 or v2 (10X Genomics) according to the manufacturer’s protocol. Paired heavy and light chain BCR libraries were prepared from the sorted B cell populations. All the steps were followed as per the manufacturer’s instructions in user guide recommended for 10X Genomics kit v1.1 or 2.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: 1
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: blood
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000178
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Donor1_MBC_2020
characteristics.characteristic: title