characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-03-31T00:17:45Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-03-31T00:17:45Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10838349
characteristics.characteristic: External Id
characteristics.text: Technische Universitat Bergakademie Freiberg Institute of Biosciences
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-31T00:17:45Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-03-31T00:17:45Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8489583
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11136:Sample 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Centrifugation of culture in late exponential phase at 8000 x g and 4째C for 15 minutes. Washing of cells with cold 10 mM H2SO4, and twice with 10 mM sodium citrate pH 7.0. Growth of L.ferriphulum in DSMZ 882 medium (pH1.8) supplemented with 20 g L-1 ferrous sulfate in a shaker at 37째C and 180 rpm. Isolation of total RNA by RNeasy Mini Kit from Qiagen. DNA removed by treatment with DNase I (New England Biolabs). Evaluation of purity and integrity of total RNA with Agilent Bioanalyzer 2100 and an RNA 600 Nano Kit (Agilent Technologies. For each sample 3 RNA preparations with RMNA integrity number above 7 from independent biological replicates pooled into one. Depletion of ribosomal RNA by MICROBExpress kit (Thermo Fisher). Preparation of cDNA library by TruSeq stranded mRNA library prep kit (Illumina).
characteristics.characteristic: description
characteristics.text: Leptospirillum ferriphilum
characteristics.characteristic: organism
characteristics.text: Leptospirillum ferriphilum
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organism part
characteristics.text: Leptospirillum ferriphilum
characteristics.characteristic: scientific_name
characteristics.text: DSM14647
characteristics.characteristic: strain
characteristics.text: Sample 2
characteristics.characteristic: title