characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-04-13T16:18:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-04-13T16:18:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11361965
characteristics.characteristic: External Id
characteristics.text: European Research Institute for the Biology of Ageing University Medical Center Groningen
characteristics.characteristic: INSDC center name
characteristics.text: 2022-04-13T16:18:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-04-13T16:18:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS9007407
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-109231638526272:BT549 IFN gamma # 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: BT549
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001096
characteristics.characteristic: cell line
characteristics.text: mammary epithelial cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0002327
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: For Reversine/DMSO treatment: BT549 cells (with according KOs) were synchronized with double thymidine block. Seven hours after thymidine release, synchronized BT549 cells were treated with DMSO or 250 nM Reversine. After 48 hours, cells were harvested using Tryple. Pelleted cells were washed with 1xDPBS before being stored as dry pellets at -20 degree Celsius. For IFN gamma treatment: BT549 cells were treated with 50 ng IFN gamma for 2 hours. After 2 hours incubation, cells were harvested and washed with 1XDPBS before being stored as dry pellet at -20 degree Celsius. For whole transcriptome analysis, RNA was isolated using the RNeasy mini kit (Qiagen) in accordance to manufacturer's protocol and RNA quality was examined using the Agilent 2100 Bioanalyzer (Agilent Technologies). 250-500 ng of total RNA was used as input for mRNA enrichment using NEXTflex Poly(A) Beads (Bio Scientific) followed by library preparation using a NEXTflex Rapid Directional qRNA-Seq Kit (Bio Scientific).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: breast ductal adenocarcinoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006318
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: mammary gland
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001911
characteristics.characteristic: organism part
characteristics.text: 2
characteristics.characteristic: replicate
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: BT549 IFN gamma # 2
characteristics.characteristic: title