characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-04-14T00:24:37Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-04-14T00:24:37Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA12921926
characteristics.characteristic: External Id
characteristics.text: Team EPIIC 'Eukaryotic Pathogens: Inflammation, T cell Immunity and Chemoresistance' Toulouse Institute for Infectious and Inflammatory Diseases (Infinity)-INSERM UMR1291 - CNRS UMR5051 - University Toulouse III
characteristics.characteristic: INSDC center name
characteristics.text: 2022-04-14T00:24:37Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-04-14T00:24:37Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10521072
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11416:SPLEEN
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HA-specific CD8-positive T cell
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Naïve HA-specific CD8+ T cells were purified from the spleen and lymph nodes of CL4-TCR F1 mice. Purified HA-specific CD8+ T cells were then cultivated with irradiated syngeneic splenocytes in the presence of HA512-520-peptide, IL-2, and IL-12. After five days of culture, living cells were collected. 3×106 activated CD8+ T cells were adoptively transferred via i.v. in female adult (5 - 7 week old) Orex-HA mice. At day 30 post-transfer, the mice were sacrificed and the HA-specific CD8+ T cells (CD11b- NK1.1- CD45.2+ CD8+ TCRb+ CD45.1+) were isolated by FACS sorting (BD FACSAria Fusion) from the spleen and hypothalami of the 13 Orex-HA mice. Immediately after cell sorting, cells were subjected to scRNA-seq processing (Chromium Single Cell 3ʹ Reagent Kits v3, CG000183 Rev C) according to the manufacturer’s instructions. Libraries were constructed using the 10X Genomics, Chromium Single Cell 3’ v3 kit including : - Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3, 16 rxns PN-1000075; Chromium Single Cell B Chip Kit, 48 rxns PN-1000073 and Chromium i7 Multiplex Kit, 96 rxns PN-120262; according to manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: adoptively transferred in Orex-HA adult female mice
characteristics.characteristic: growth condition
characteristics.text: CCD11b-; NK1.1-; CD45.2+; CD8+; TCRb+; CD45.1+
characteristics.characteristic: immunophenotype
characteristics.text: pool of 13 mice
characteristics.characteristic: individual
characteristics.text: activated CD8-positive, alpha-beta T cell
characteristics.characteristic: inferred cell type
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: spleen; lymph node
characteristics.characteristic: organism part
characteristics.text: spleen
characteristics.characteristic: sampling site
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: CL4-TCR
characteristics.characteristic: strain
characteristics.text: 30
characteristics.unit: day
characteristics.characteristic: time
characteristics.text: SPLEEN
characteristics.characteristic: title