characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-04-29T00:13:34Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-04-29T00:13:34Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11114532
characteristics.characteristic: External Id
characteristics.text: Section of Bioinformatics and Systems Cardiology, Klaus Tschira Institute for Integrative Computational Cardiology and Department of Internal Medicine III, University Hospital Heidelberg.
characteristics.characteristic: INSDC center name
characteristics.text: 2022-04-29T00:13:34Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-04-29T00:13:34Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8764158
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11171:HAoEC_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: human aortic endothelial cells
characteristics.characteristic: cell line
characteristics.text: endothelial cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000115
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Endothelial cells were purchased from Lonza (HAoEC: CC-2535, HUVEC: CC-2935, HCMEC: CC-7030) and HUVECs were cultured in endothelial basal medium (EBM; CC-3156, Lonza) supplemented with 10% fetal calf serum (FCS; 4133, Invitrogen), amphotericin-B, ascorbic acid, bovine brain extract, endothelial growth factor, gentamycin sulfate, and hydrocortisone (EGM-Bullet Kit; CC-3124, Lonza) while HCMEC and HAoEC were cultered in EBM-2 supplemented with 10% FCS, hydrocortisone, FGF, VEGF, R3-IGF, ascorbic acid, EGF and GA-1000 (EGM-2-Bullet kit; CC-3162, Lonza) at humidified atmosphere, at 37 °C/5% CO2 Total RNA lysates were isolated using the commercial Qiagen miRNeasy Kit (217004, Qiagen). RNase R treatment was performed with 10 µg of total RNA incubated for 15 minat 37°C with 10 U RNase R (RNR-07250, epicentre) in 1x RNase R digestion buffer (RNR-07250, epicentre). RNA was purified using the commercial Qiagen RNeasy MinElute CleanUp Kit (74204). RNA libraries were obtained with the TruSeq Stranded Total RNA Sample Prep Kit.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: aorta
characteristics.characteristic: organism part
characteristics.text: HAoEC_2
characteristics.characteristic: replicate
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: HAoEC_2
characteristics.characteristic: title