characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-04-26T16:19:20Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-04-26T16:19:20Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11299159
characteristics.characteristic: External Id
characteristics.text: Gehring Lab Institute for Genetics University of Cologne
characteristics.characteristic: INSDC center name
characteristics.text: 2022-04-26T16:19:20Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-04-26T16:19:20Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8947336
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11184:156271
characteristics.characteristic: Submitter Id
characteristics.text: 31
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HeLa Flp-In-T-REx
characteristics.characteristic: cell line
characteristics.text: epithelial cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000066
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: HeLa Flp-In-T-REx (established by Elena Dobrikova and Matthias Gromeier, Duke University Medical Center) WT and UPF3A overexpressing cells were maintained at 37° C, 5% CO2 and 90% humidity in Dublecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific) supplemented with 9% fetal bovine serum (FBS) and Penicillin-Streptomycin (both Thermo Fisher Scientific). To induce expression of the stably integrated UPF3A construct, 30 μg/ml cumate was added and the cells were harvested after 48h. Total RNA was extracted using the Direct-zol RNA MiniPrep kit including the recommended DNase I treatment (Zymo Research) according to the manufacturer's instructions. Ribosomal depletion and strand specific library preparation was carried out with TruSeq Stranded Total RNA (with Ribo-Zero Gold) according to the manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: cervical adenocarcinoma
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001416
characteristics.characteristic: disease
characteristics.text: African American
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/HANCESTRO_0568
characteristics.characteristic: ethnic group
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: uterine cervix
characteristics.characteristic: organism part
characteristics.text: ERCC spike in
characteristics.characteristic: protocol
characteristics.text: 2
characteristics.characteristic: replicate
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: 156271
characteristics.characteristic: title