characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-03-11T00:12:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-04-06T17:31:07Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13197460
characteristics.characteristic: External Id
characteristics.text: University of Jyvaskyla
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-11T00:12:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-04-06T17:31:07Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10800975
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11370:Sample 6
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: After 14 days of cultivation, 50 ml of every culture were centrifugated for 5 min (2500×g, 18 °C), after which the supernatant was discarded. Cell pellets were resuspended in 700 μl of DNA/RNA Shield™ and homogenized in BashingBead™ (0.1 & 0.5 mm) lysis tubes (Zymo Research, Irvine, CA, USA) using a TerraLyzer™ Cell Disruptor. Cryptomonas sp. was grown under phototrophic conditions, glucose suplemented phototrophic conditions and 3 different dissolved organic carbon (DOC) concentratios: 1.5, 30 and 90 mg C l−1 (see materials and methods). RNA was extracted using a Chemagic™ 360 and the Chemagic™ Viral DNA/RNA 300 Kit H96 following manufacturer’s instructions (PerkinElmer, Waltham, USA). Extracted RNA was treated with DNAse I (Thermo Fisher Scientific), purified using Zymo spin-columns and quantified using a NanoDrop (Thermo Fisher Scientific). Samples were stored at −80 °C. RNA library generation and transcriptome sequencing of the selected treatments was conducted by Novogene Co., LTD (Cambridge, UK)
characteristics.characteristic: description
characteristics.text: DOC1.5
characteristics.characteristic: growth condition
characteristics.text: Cryptomonas sp. CPCC 336
characteristics.characteristic: organism
characteristics.text: Cryptomonas sp. CPCC 336
characteristics.characteristic: organism
characteristics.text: Cryptomonas sp. CPCC 336
characteristics.characteristic: scientific_name
characteristics.text: Cryptomonas sp. strain CPCC 336
characteristics.characteristic: strain
characteristics.text: Sample 6
characteristics.characteristic: title