characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-04-13T00:22:00Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-04-13T00:22:00Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13376272
characteristics.characteristic: External Id
characteristics.text: Wellcome Sanger Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2022-04-13T00:22:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-04-13T00:22:00Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10978786
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11536:Sample 43
characteristics.characteristic: Submitter Id
characteristics.text: 61 to 65
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: inferred cell
characteristics.characteristic: cell type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Briefly, solid tissues were transferred to a 100mm tissue culture dish, cut into small pieces and transferred to C-tubes (Miltenyi Biotec) at a maximum of 5g/tube in 5mL of X-vivo15 media containing 0.13U/m Liberase TL (Roche), 10U/mL DNase (benzonase nuclease, Millipore/Merck) supplemented with 2% (v/v) heat-inactivated fetal bovine serum (FBS; Gibco), penicillin (100 U/ml, Sigma-Aldrich), streptomycin (0.1 mg/ml, Sigma-Aldrich), and 10mM HEPES (Sigma Aldrich). The samples were then dissociated using a GentleMACS Octo dissociator (Miltenyi Biotec) using a protocol that provided gradual ramping up of homogenisation speed along with 2x 15 minute heating/mixing steps at 37°C. Resulting single cell suspension was layered over ficoll and spun at 400g for 20 minutes, with no brake. The interface layer containing the MNCs were collected and transferred to a fresh tube and washed in PBS+BSA. Blood and bone marrow MNC were washed with PBS and exposed to the same enzymatic conditions as solid tissues by resuspending cell pellets in tissue dissociation media containing liberase TL for 30 minutes at 37°C prior to counting and resuspending in 10X buffer. This protocol applies to donors A29, A31, A35, A36, A37, A52 Following the manufacturer's instructions for Chromium Single Cell 5’ V(D)J Reagent Kits (v1 Chemistry)
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Sample 43
characteristics.characteristic: emulsion id
characteristics.text: A35
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: dead
characteristics.characteristic: organism status
characteristics.text: Sample 43
characteristics.characteristic: pool id
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: Sample 43
characteristics.characteristic: title