characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-03-31T00:18:29Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-03-31T00:18:29Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13409733
characteristics.characteristic: External Id
characteristics.text: INSERM U1301 CNRS 5070 EFS/Univ Paul Sabatier
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-31T00:18:29Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-03-31T00:18:29Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS11012202
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11542:Delta13
characteristics.characteristic: Submitter Id
characteristics.text: 18 to 20
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Liver biopsies were harvested from control flox and Hmgb1 liver knockout mice in chow diet or after fasting/refeeding challenge Flash-frozen liver biopsies were sent to Active Motif to perform the ATAC-seq assay. The tissue was manually dissociated, isolated nuclei were quantified using a hemocytometer, and 100,000 nuclei were tagmented as previously described (58), with some modifications based on (59) using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen), amplified with 10 cycles of PCR, and purified using Agencourt AMPure SPRI beads (Beckman Coulter). Resulting material was quantified using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: chow
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001461
characteristics.characteristic: diet
characteristics.text: HMGB1ΔHep
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: Delta13
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000173
characteristics.characteristic: title