characteristics.text: 0
characteristics.characteristic: 16S rRNAs recovered
characteristics.text: FALSE
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009639
characteristics.characteristic: 16S recovered
characteristics.text: barrnap;0.9;--kingdom bac
characteristics.characteristic: 16S recovery software
characteristics.text: 0
characteristics.characteristic: 23S rRNAs recovered
characteristics.text: 0
characteristics.characteristic: 5S rRNAs recovered
characteristics.text: 0
characteristics.characteristic: CheckM strain heterogeneity
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-04-01T08:27:13Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-04-01T08:27:13Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13477016
characteristics.characteristic: External Id
characteristics.text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Blautia_A;s__Blautia_A massiliensis
characteristics.characteristic: GTDB r202 classification
characteristics.text: Stanford University School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2022-04-01T08:27:13Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-04-01T08:27:13Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: 19.62
characteristics.characteristic: MAG coverage depth
characteristics.text: Reads (ERR7746184) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4561544) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.
characteristics.characteristic: MAG coverage software
characteristics.text: ERS11079067
characteristics.characteristic: SRA accession
characteristics.text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-A-D_N_5_1522.208
characteristics.characteristic: Submitter Id
characteristics.text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics.
characteristics.characteristic: assembly quality
characteristics.text: metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz
characteristics.characteristic: assembly software
characteristics.text: Reads from ERR7746184 were assembled into contigs (ERZ4561544). The following read sets from this study were the most similar to ERR7746184 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7746184,ERR7738610,ERR7738200,ERR7745815,ERR7738569,ERR7738586,ERR7747426,ERR7738608,ERR7738559,ERR7746321. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4561544. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.
characteristics.characteristic: binning parameters
characteristics.text: MetaBAT2 v2.15; MAGpurify v2.1.0
characteristics.characteristic: binning software
characteristics.text: 2014-01-26
characteristics.characteristic: collection date
characteristics.text: CheckM marker lineage: k__Bacteria (UID203)
characteristics.characteristic: completeness approach
characteristics.text: 57.89
characteristics.characteristic: completeness score
characteristics.text: CheckM;1.1.2;lineage_wf
characteristics.characteristic: completeness software
characteristics.text: 0
characteristics.characteristic: contamination score
characteristics.text: reads
characteristics.characteristic: contamination screening input
characteristics.text: All reads in ERR7746184 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).
characteristics.characteristic: contamination screening parameters
characteristics.text: This sample represents an uncultured Blautia sp. MAG assembled and binned from ERR7746184.
characteristics.characteristic: description
characteristics.text: terrestrial biome
characteristics.characteristic: environment (biome)
characteristics.text: human-associated habitat
characteristics.characteristic: environment (feature)
characteristics.text: feces
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCIT_C13234
characteristics.characteristic: environment (material)
characteristics.text: Tanzania
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 1287
characteristics.characteristic: geographic location (elevation)
characteristics.text: -3.55
characteristics.characteristic: geographic location (latitude)
characteristics.text: -34.58
characteristics.characteristic: geographic location (longitude)
characteristics.text: GUT_GENOME000676
characteristics.characteristic: inter-study species representative
characteristics.text: ERR7746345_bin.127
characteristics.characteristic: intra-study species representative
characteristics.text: metagenome-assembled genome
characteristics.characteristic: investigation type
characteristics.text: human feces
characteristics.characteristic: isolation source
characteristics.text: human gut metagenome
characteristics.characteristic: metagenomic source
characteristics.text: https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103
characteristics.characteristic: nucleic acid extraction
characteristics.text: 22
characteristics.characteristic: number of standard tRNAs extracted
characteristics.text: uncultured Blautia sp.
characteristics.characteristic: organism
characteristics.text: Metagenome sequencing of Hadza gut microbiome
characteristics.characteristic: project name
characteristics.text: No
characteristics.characteristic: reassembly post binning
characteristics.text: https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103
characteristics.characteristic: reference for biomaterial
characteristics.text: https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103
characteristics.characteristic: sample collection device or method
characteristics.text: ERS2585777
characteristics.characteristic: sample derived from
characteristics.text: uncultured Blautia sp.
characteristics.characteristic: scientific_name
characteristics.text: Illumina NovaSeq 6000
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0008637
characteristics.characteristic: sequencing method
characteristics.text: tRNAscan-SE;2.0.9;-B
characteristics.characteristic: tRNA extraction software
characteristics.text: GTDB-TK v1.7.0, r202 database, default parameters
characteristics.characteristic: taxonomic classification
characteristics.text: multi-marker approach
characteristics.characteristic: taxonomic identity marker
characteristics.text: ERR7746184_bin.208
characteristics.characteristic: title
characteristics.text: 11
characteristics.characteristic: unique standard tRNAs extracted