characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-03-31T00:18:23Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-03-31T00:18:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13093306
characteristics.characteristic: External Id
characteristics.text: Fraunhofer Institute for Molecular Biology and Applied Ecology
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-31T00:18:23Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-03-31T00:18:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10697309
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11459:R1488
characteristics.characteristic: Submitter Id
characteristics.text: 7
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: For each sample, 25 mg of plant tissue were randomly collected from the test vessel into a tube. After adding the lysis matrix (¼ Ceramic Sphere, MP Biomedicals), the lysis was performed according to the manufacturer’s protocol of the RapidPURE RNA Plant Kit (MP Biomedicals Illkirch, France). Homogenization was conducted at 5 m/s for 60 s with FastPrep-24© 5G (MP Biomedicals Illkirch, France) at room temperature. One week before test start, a pre-culture of Lemna minor was auxenically prepared from a stock culture in Steinberg medium according to OECD TG 221. Plants were kept in a growth chamber at 24±2°C and under continuous light (85-135 µE∙m-2∙s-1). Atorvastatin-calcium was purchased from abcr (Karlsruhe, Germany). Test solutions of EC5 and EC20 (% inhibition of growth (e.g. 50 %) is determined and expressed as the ECx e.g. EC50) determined in preliminary experiments according to OECD TG 221, were prepared with Steinberg medium at a pH 5.5±0.2. 150 mL of each test solution were transferred into three test vessels (replicates) and analogously 150 mL of pure Steinberg medium were used as control samples. Four healthy plants with three fronds each were transferred from the pre-culture to the test vessels. Incubation was conducted according to the conditions described in “Growth protocol” under random arrangement for 3 days. Total RNA and protein were extracted according to the RapidPURE Plant Kit (MP Biomedicals Illkirch, France). RNA quantification (RNA>100 ng/µL) was performed using Nanodrop 2000 spectrophotometer (Thermo Scientific), and quality control (RIN>7.0) was conducted using the 2100 Bioanalyzer (Agilent Technologies). The samples were stored at -80°C until mRNA sequencing. cDNA libraries were prepared from mRNA that was purified through PolyA selection using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were validated using a Fragment Analyzer system (Agilent, Santa Clara, USA) before sequencing.
characteristics.characteristic: description
characteristics.text: growth stage
characteristics.characteristic: developmental stage
characteristics.text: 31.05.2021
characteristics.characteristic: extraction date
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Lemna minor
characteristics.characteristic: organism
characteristics.text: Lemna minor
characteristics.characteristic: organism
characteristics.text: whole plant
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organism part
characteristics.text: 7.7
characteristics.characteristic: rin
characteristics.text: Lemna minor
characteristics.characteristic: scientific_name
characteristics.text: R1488
characteristics.characteristic: title
characteristics.text: EC5
characteristics.characteristic: treatment