characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-04-30T00:12:58Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-04-30T00:12:58Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13469008
characteristics.characteristic: External Id
characteristics.text: Berlin Institute for Medical Systems Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2022-04-30T00:12:58Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-04-30T00:12:58Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS11071069
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11569:shYTHDF123_Neurite_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: neuron of cerebral cortex
characteristics.characteristic: cell type
characteristics.text: neurite
characteristics.characteristic: cellular component
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Primary neuron cultures were prepared from C57B/6 wild type mice (P0) pups and cultured as previously described (Kaech and Banker, 2006). RNA was extracted from Trifast lysate using Directzol column purification kit (zymo) including DNaseI treatment procedure; RNA samples were quantified and analysed for integrity by Bioanalyser; Good separation was verified by RT-qPCR. (2.5-3.7 cycles difference in Taglin between soma/neurite samples) Stranded mRNA Library kit; 1ul of 1:100 ERCC1 spike-in was added to 49ul initial solution with 400ng total RNA.; Final PCR was done with 10-15 cycles’ reaction.
characteristics.characteristic: description
characteristics.text: neonate
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: brain
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: shYTHDF123_Neurite_1
characteristics.characteristic: title