characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-04-30T00:16:11Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-04-30T00:16:11Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA12813764
characteristics.characteristic: External Id
characteristics.text: HENAN AGRICULTURAL UNIVERSITY
characteristics.characteristic: INSDC center name
characteristics.text: 2022-04-30T00:16:11Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-04-30T00:16:11Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10420724
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11413:Sample 4
characteristics.characteristic: Submitter Id
characteristics.text: 6 days after germination
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: C7-2t
characteristics.characteristic: cultivar
characteristics.text: Protocols: Maize mesocotyls were collected from 6-d etiolated seedlings after 10% PEG 6000 treatment of roots for 60 min for RNA extraction. Maize seeds were germinated at 28oC and grown in Hogland’s nutrient solution in the dark for 6 days and subjected to PEG treatment. CTAB PBIOZOL and ethanol precipitation protocol was used for extraction and purification of total RNA from maize mesocotyl tissue. mRNA was fragmented and enriched with by oligo-dT beads magnetic beads. Sequencing library was amplified with the broken mRNA as the templates to synthesize cDNA. The cDNA templates were purified with the kit and enriched by PCR.
characteristics.characteristic: description
characteristics.text: seedling development stage
characteristics.characteristic: developmental stage
characteristics.text: etiolation
characteristics.characteristic: growth condition
characteristics.text: Zea mays
characteristics.characteristic: organism
characteristics.text: Zea mays
characteristics.characteristic: organism
characteristics.text: mesocotyl
characteristics.characteristic: organism part
characteristics.text: Zea mays
characteristics.characteristic: scientific_name
characteristics.text: 10 percent PEG 6000 for 60 min
characteristics.characteristic: stimulus
characteristics.text: Sample 4
characteristics.characteristic: title