characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-04-21T00:32:07Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-04-21T00:32:07Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13254173
characteristics.characteristic: External Id
characteristics.text: Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg
characteristics.characteristic: INSDC center name
characteristics.text: 2022-04-21T00:32:07Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-04-21T00:32:07Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10857216
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11505:Sv2aHET4S5
characteristics.characteristic: Submitter Id
characteristics.text: 6
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: zebrafish
characteristics.characteristic: common name
characteristics.text: Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). TruSeq RNA-Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.
characteristics.characteristic: description
characteristics.text: larval stage
characteristics.characteristic: developmental stage
characteristics.text: sv2a+/-
characteristics.characteristic: genotype
characteristics.text: pool of 10 heads
characteristics.characteristic: individual
characteristics.text: Danio rerio
characteristics.characteristic: organism
characteristics.text: Danio rerio
characteristics.characteristic: organism
characteristics.text: head
characteristics.characteristic: organism part
characteristics.text: Danio rerio
characteristics.characteristic: scientific_name
characteristics.text: not available
characteristics.characteristic: sex
characteristics.text: AB
characteristics.characteristic: strain
characteristics.text: Sv2aHET4S5
characteristics.characteristic: title