characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-03-31T00:18:29Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-03-31T00:18:29Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13410165
characteristics.characteristic: External Id
characteristics.text: INSERM U1301 CNRS U5070 EFS/Univ Paul Sabatier
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-31T00:18:29Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-03-31T00:18:29Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS11012630
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11543:Flox14
characteristics.characteristic: Submitter Id
characteristics.text: 18 to 20
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Liver from control (flox) or Hmgb1 liver knockout mice after a metabolic stress either induced by a 12-week high fat diet consumption or a fasting (6hours)-refeeding (8hours) challenge. Briefly, frozen liver biopsies (100-200 mg) harvested from HMGB1fl/fl and HMGB1ΔHep mice under CD, upon HFD60% or after F/R, were minced and fixed at room temperature in PBS-1% formaldehyde (Sigma-Aldrich, 47608, Saint Louis, MO, USA) for 20 minutes. After sonication, chromatin immunoprecipitation was performed using anti-HMGB1 antibody (Abcam, ab18256, Cambridge, UK). Immunoprecipitated DNA was subjected to library preparation using NEBNext DNA Ultra II kit.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: high fat diet
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002757
characteristics.characteristic: diet
characteristics.text: HMGB1 fl/fl
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: Flox14
characteristics.characteristic: title