characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-05-01T00:14:21Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-05-01T00:14:21Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13166524
characteristics.characteristic: External Id
characteristics.text: Section of Genetics and Genomics, Department of Metabolism, Digestion and Reproduction, Imperial College London
characteristics.characteristic: INSDC center name
characteristics.text: 2022-05-01T00:14:21Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-05-01T00:14:21Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10770103
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11474:sgControl2_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: MIN6
characteristics.characteristic: cell line
characteristics.text: beta cell
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: The MIN6-SAM cell line was generated by successive transduction of MIN6 mouse  cells with lentivirus dCAS-VP64_Blast (#61425, Addgene) followed by blasticidin selection (1 µg/mL) and lentivirus MS2-P65-HSF1_Hygro (#61426, Addgene) followed by hygromycin selection (100 µg/mL). MIN6-SAM cells were subsequently transduced with the sgRNA expressing vector lenti sgRNA-(MS2)-zeo (#61427, Addgene). RNA from technical triplicates of 2 independent Hnf1a activating sgRNAs and 2 independent control sgRNAs was extracted. RNA was prepared using RNeasy mini kit (Qiagen) including DNAse I (Qiagen) treatment. RNA-seq libraries were prepared using the Truseq Stranded mRNA Sample Prep Kit (Illumina) following manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: CRISPR/Cas9 control with g2 RNA
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: pancreas
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: C57BL/6 IT6
characteristics.characteristic: strain
characteristics.text: sgControl2_1
characteristics.characteristic: title