characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-06T12:23:35Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-06T12:23:35Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA12941371
characteristics.characteristic: External Id
characteristics.text: Centre for Discovery Brain Sciences University of Edinburgh
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-06T12:23:35Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-06T12:23:35Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10540164
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11437:hPSC1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: hPSC1
characteristics.characteristic: cell line
characteristics.text: neural cell
characteristics.characteristic: cell type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Organoids were collected in 1.5ml Eppendorff tubes and washed three times in 1xPBS. The supernatant of the final wash was discarded and organoids were shock frozen on dry ice and stored at -80C. Total RNA was extracted using the QIAGEN RNeasy Plus Micro kit as per manufacturer’s instructions. RNA was eluated in 14 μl of RNAse free water. Libraries were prepared from 250ng of each total-RNA sample using the NEBNEXT Ultra II Directional RNA Library Prep kit (NEB #7760) and the Poly-A mRNA magnetic isolation module (NEB #E7490) according to the provided protocol
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: organoid culture
characteristics.characteristic: growth condition
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: inner cell mass
characteristics.characteristic: organism part
characteristics.text: embryonic stem cell
characteristics.characteristic: progenitor cell type
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: hPSC1
characteristics.characteristic: title