characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-05-16T00:14:57Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-05-16T00:14:57Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13009519
characteristics.characteristic: External Id
characteristics.text: Karolinska Institutet Department of Cell & Molecular Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2022-05-16T00:14:57Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-05-16T00:14:57Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10608270
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11452:PBMCs_run3
characteristics.characteristic: Submitter Id
characteristics.text: 47
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: peripheral blood mononuclear cell
characteristics.characteristic: cell type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: hPBMCs were gently thawed and stained with PE Mouse Anti-Human CCR7 (2-L1-A), PE-Cy7 Mouse Anti-Human CD4 (SK3), FITC Mouse Anti-Human CD45RA (HI100), PerCP-Cy5.5 / BB700 Mouse Anti-Human CD8 (RPA-T8), PE-Cy5 Mouse Anti-Human CD45RO (UCHL1) (BD Biosciences) before sorting. Dead cells were gated out after staining with propidium iodide (Thermo Fisher). Live, single cells were sorted into 384-well plates containing lysis buffer using a BD FACSMelody equipped with a 100-µm nozzle and plate cooling with index sorting on (BDBiosciences). No extraction was performed. Libraries were constructed using the Smart-seq3xpress protocol using either KAPA or SeqAmp Polymerase as annotated in the submitted metadata file.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: donor3
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: PBMCs_run3
characteristics.characteristic: title