characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-01T00:11:18Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-01T00:11:18Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13318185
characteristics.characteristic: External Id
characteristics.text: The Francis Crick Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-01T00:11:18Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-01T00:11:18Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10920924
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11514:shAldh18a1_R2_TRCN0000350725
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: 46C
characteristics.characteristic: cell line
characteristics.text: embryonic stem cell
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: About three days after transducing the cells with shRNAs, the cells were pulsed with 4sU for 15 minutes. After pulsing the cells with 4sU, total RNA was purified by Trizol protocol. Yeast RNA was added to the samples as Spike-in control. RNA was fragmented, and 4sU labelled RNA were biotinylated. Then biotinylated RNA was captured using streptavidin beads. RNA was eluted by DTT, and finally purified by miRNeasy mini elute column. The libraries were constructed using NEBNext Ultra II Directional RNA Library Prep Kit according to the manual.
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: inner cell mass
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: 129 mouse strain
characteristics.characteristic: strain
characteristics.text: shAldh18a1_R2_TRCN0000350725
characteristics.characteristic: title