characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-01T00:13:58Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-01T00:13:58Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA12786536
characteristics.characteristic: External Id
characteristics.text: IP
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-01T00:13:58Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-01T00:13:58Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10392712
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11383:ZCF26_null_3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: RNAs were isolated from different strains of C. albicans using the RNeasy mini kit mirVana RNA isolation kit (Cat. No. 74104, Qiagen) C. albicans strains were grown in YPD media either in planktonic or biofilm growth conditions. Total RNA was isolated from four independent planktonic or biofilm cultures for each strain. Planktonic cells were grown in YPD medium at 30°C till OD600=0.8 whereas biofilm was grown in YPD media at 37°C in YPD media for 18h. Cells were pelleted down at 4000 rpm and washed 3 times with 1xPBS. Cells were resuspended in extraction buffer and lysed by adding glass beads. Bead-beater was used to break the cells. The RNeasy columns were used to isolate the total RNA. To remove the potential contaminating chromosomal DNA, RNA samples were treated with DNAse (Cat. No. 79254, Qiagen). Libraries were built using a TruSeq Stranded mRNA library Preparation Kit (Illumina, USA) following the manufacturer’s protocol.
characteristics.characteristic: description
characteristics.text: ZCF26_null
characteristics.characteristic: line
characteristics.text: Candida albicans WO-1
characteristics.characteristic: organism
characteristics.text: Candida albicans WO-1
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Candida albicans WO-1
characteristics.characteristic: scientific_name
characteristics.text: ZCF26_null_3
characteristics.characteristic: title