characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-05-10T01:04:44Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-05-10T01:04:44Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA12927641
characteristics.characteristic: External Id
characteristics.text: Department II of Internal Medicine and Center for Molecular Medicine Cologne, University of Cologne
characteristics.characteristic: INSDC center name
characteristics.text: 2022-05-10T01:04:44Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-05-10T01:04:44Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10526775
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11428:154680
characteristics.characteristic: Submitter Id
characteristics.text: 14
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Undamaged murine kidneys were withdrawn after anesthesia by ketamin/xylazine. RNA isolation of total RNA was performed using commercial homogenization (Bio 101 FastPrep FP120-120V, Savant, Midland, MI) and the RNeasy mini kit (QIAGEN, Hilden, Germany). RNA sequencing was done by the Cologne Center for Genomics using the Ribo-Zero Human/Mouse/Rat preparation kits and the TruSeq Stranded Total RNA samples (Illumina Inc., San Diego, CA). Library preparation was done by the Cologne Center for Genomics using the TruSeq Stranded mRNA Library Prep Kit with an additional ERCC-spike-in.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: calorie restriction
characteristics.characteristic: diet
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: 3
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: kidney
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6N
characteristics.characteristic: strain
characteristics.text: 154680
characteristics.characteristic: title