characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-05-02T00:14:51Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-05-02T00:14:51Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13137565
characteristics.characteristic: External Id
characteristics.text: The Azrieli Center for Stem Cells and Genetic Research, Department of Genetics, The Alexander Silberman Institute of Life Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2022-05-02T00:14:51Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-05-02T00:14:51Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10741502
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11465:FZD4 heterozygous 3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: h-PES10 and h-pES12 fused
characteristics.characteristic: cell line
characteristics.text: embryonic stem cell
characteristics.characteristic: cell type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were trypsinized and kept in -80 until RNA extraction. Colonies formed out of single cells were collected and plated on mouse feeder cells in 6-well plates. RNA was extracted using NucleoSpin RNA Plus kit mRNA fraction of total RNA was enriched by pulldown of poly(A)-RNA. RNA sequencing libraries were generated using TruSeq RNA Library Prep Kit (Illumina) according to the manufacturer's protocol.
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: Heterozygous mutation FZD4
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blastocyst
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: FZD4 heterozygous 3
characteristics.characteristic: title