characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-01T00:11:11Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-01T00:11:11Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA12120192
characteristics.characteristic: External Id
characteristics.text: IP
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-01T00:11:11Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-01T00:11:11Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS9766825
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11237:4688
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: isolated from nasal swab
characteristics.characteristic: clinical information
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Nasal swab from patients with symptoms of SARS-CoV-2 RNA extraction and qRT-PCR were carried out by the French National Center for Respiratory infection virus (32156327). Double-stranded cDNA was synthetized using random hexamers and the ProtoScript II first strand cDNA synthesis kit followed by the NEBNext Ultra II Non Directional RNA Second Strand Synthesis Module from New England Biolabs. Indexed library preparation was made using the Nextera Flex for enrichment (Illumina) kit following the manufacturer’s protocol without modification. Indexed libraries were pooled according to the results of the RT-qPCR CT for SARS-CoV-2 of each sample. For the Twist hybridization reactions, a modified Nextera Flex enrichment protocol was used.
characteristics.characteristic: description
characteristics.text: Severe acute respiratory syndrome coronavirus 2
characteristics.characteristic: infect
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: nasal cavity
characteristics.characteristic: organism part
characteristics.text: Twist Bioscience panviral hybridisation
characteristics.characteristic: protocol
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 4688
characteristics.characteristic: title
characteristics.text: CT32
characteristics.characteristic: viral load