characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-05-31T00:13:24Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-05-31T00:13:24Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11005683
characteristics.characteristic: External Id
characteristics.text: UCD School of Biomolecular and Biomedical Science
characteristics.characteristic: INSDC center name
characteristics.text: 2022-05-31T00:13:24Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-05-31T00:13:24Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8655464
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11149:A5
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: wtsli028-a
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: hiPSCs were differentiated using a modified version of the Takasato et al. 2016 protocol. Briefly, hiPSCs were differentiated as a cell monolayer using CHIR99021 and FGF9 treatments for 7 days to induce intermediate mesoderm. Cells were subsequently pelleted and grown in a suspension culture for 48 hours. Organoids were then encapsulated into either synthetic self-assembling peptide hydrogels of variable mechanical strengths or Matrigel and differentiated until day 24. 6 organoids per support matrix were then pooled and dissociated using the cold-active protease from Bacillus licheniformis for scRNA-seq experiments. Following dissociation, single cell suspensions were loaded onto the Chromium Next GEM Chip G (Cat number: 1000127) with reverse transcriptase master mix and Gel Bead Kit (Cat number: 1000128) to achieve cell recoveries of 3,000-4,000 cells. GEMs (gel beads in emulsion) were formed as per manufacturer’s instructions using the 10X Chromium Controller and Single-cell 3’ v3 kits. Libraries were prepared using the Chromium Single Cell 3' v3 Library and Gel Bead Kit (Cat number: 1000128) and Single Index Kit T Set A (Cat number: 1000213) as per manufacturers (10X Genomics) instructions.
characteristics.characteristic: description
characteristics.text: Alpha5
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000171
characteristics.characteristic: gel name
characteristics.text: Manchester BIOGEL
characteristics.characteristic: gel supplier
characteristics.text: Synthetic
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/MI_0794
characteristics.characteristic: gel type
characteristics.text: kidney organoid culture
characteristics.characteristic: growth condition
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: induced pluripotent stem cell
characteristics.characteristic: progenitor cell type
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: A5
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002103
characteristics.characteristic: title