characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-05-20T00:12:18Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-05-20T00:12:18Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA12943015
characteristics.characteristic: External Id
characteristics.text: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
characteristics.characteristic: INSDC center name
characteristics.text: 2022-05-20T00:12:18Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-05-20T00:12:18Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10541804
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11438:fNDC#1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: MUNIi008-A
characteristics.characteristic: cell line
characteristics.text: neural cell
characteristics.characteristic: cell type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: For each scRNA-seq sample, 18 COs were used. Whole organoids were washed in HBSS, cut into pieces and placed into collagenase P dissolved in HBSS at 0.5 mg/ml. Dissociation was performed in a shaking incubator for 15 min at 37 °C with occasional pipetting, followed by ten-fold dilution with cold 2% FBS in HBSS and filtering through 40 µm cell strainer. Cells were centrifuged and resuspended in 500 μl of 2% FBS in HBSS. The cell suspension was stained for viability with 7-AAD, and 500,000 viable cells were sorted. Single-cell partitioning and cDNA preparation were done using Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (10x Genomics) according to the manufacturer’s protocol Sequencing library preparation was done according to the manufacturer’s protocol (Chromium Single Cell 3' Reagent Kits User Guide v3.1).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: cerebral organoid culture
characteristics.characteristic: growth condition
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: induced pluripotent stem cell
characteristics.characteristic: progenitor cell type
characteristics.text: 60
characteristics.unit: day
characteristics.characteristic: sampling time point
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: fNDC#1
characteristics.characteristic: title