characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-09T00:14:55Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-09T00:14:55Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110055396
characteristics.characteristic: External Id
characteristics.text: Quadram Institute Bioscience
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-09T00:14:55Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-09T00:14:55Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12157496
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11802:Sample 26
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: Protocols: Selection of transposon-insertion mutants in the presence of meropenem was performed in duplicate. Approximately 10^7 cells from the library were grown overnight at 37 °C in 1 mL of lysogeny broth containing meropenem at 2×, 1×, 0.5×, and 0.25× the MIC of 0.032 mg.mL−1. Control cultures without meropenem were grown in parallel. For each concentration of meropenem, cultures were grown in which 0 (uninduced), 0.2 or 1.0 mM IPTG was added. Genomic DNA was extracted using a Quick-DNA Fungal/Bacterial 96 kit (Zymo Research) according to the manufacturer's instructions. DNA was fragmented using a Nextera tagmentation kit (Illumina). The fragmented DNA was amplified by PCR using oligonucleotide primers that hybridised at the transposon ends and contained an Illumina adapter sequence at their 5′ end.
characteristics.characteristic: description
characteristics.text: Escherichia coli K-12
characteristics.characteristic: organism
characteristics.text: Escherichia coli K-12
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_83333
characteristics.characteristic: organism
characteristics.text: 2
characteristics.characteristic: replicate
characteristics.text: Escherichia coli K-12
characteristics.characteristic: scientific_name
characteristics.text: BW25113
characteristics.characteristic: strain
characteristics.text: Sample 26
characteristics.characteristic: title