characteristics.text: 2024-02-14
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Medical University of Vienna
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS18270132
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13804:CDL_0002
characteristics.characteristic: Submitter Id
characteristics.text: 22
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Animal experiments were performed in accordance to the approval by the Austrian Federal Ministry of Education, Science and Research (BMBWF-V/3b/2020-0.398.007). The organs of Mus musculus C57BL/6J were collected after acquiring the study measurements and following euthanasia under anesthesia between mid-May and mid-June 2021, and stored at -80°C prior to RNA extraction.  Fibrosis was induced by the administration of carbon tetrachloride (CCl4) or thioacetamide (TAA). CCl4 was gavaged as a 20% v/v solution in olive oil at a dose of 2 mL/kg body weight three times a week. TAA was injected intraperitoneally in 0.9% saline at a dose of 150 mg/kg thrice a week. The dose was gradually increased over the first week for CCl4 administration and over the first two weeks for TAA administration. The total time of fibrosis induction was 12 weeks, followed by one or two weeks of regression.  Each sample represents total RNA from murine liver, extracted using QIAGEN RNA Easy Mini Kit, and stored in -80°C prior to submission. The library preparation was done with a TruSeq Stranded mRNA LT sample preparation kit (Illumina, CA, USA).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: CDL_0002
characteristics.characteristic: individual
characteristics.text: control (olive oil)
characteristics.characteristic: injury
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: CCL4_HC
characteristics.characteristic: sample group
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: CDL_0002
characteristics.characteristic: title