characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-05-06T00:32:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-05-06T00:32:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13421378
characteristics.characteristic: External Id
characteristics.text: Departments of Physiology and Medicine University of Toronto
characteristics.characteristic: INSDC center name
characteristics.text: 2022-05-06T00:32:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-05-06T00:32:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS11023514
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11551:Sample 7
characteristics.characteristic: Submitter Id
characteristics.text: 13
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Fecal pellets were collected from the colon. Mice were fed a high-fat diet (HFD; 60% calories from fat; D12492, Research Diets) for 6 weeks to achieve a state of diet-induced obesity, with a sucrose-matched regular chow control diet (CON; 10% calories from fat; D12450J, Research Diets) used as control. A subset of animals in both diet groups was supplemented with 0.3% w/w nobiletin (R&S PharmChem). DNA was extracted from samples using the ZymoBIOMICS DNA miniprep kit (ZymoBIOMICS). The V4 hypervariable region of the 16S rRNA gene was amplified using uniquely barcoded 515F (forward) and 806R (reverse) sequencing primers to allow for multiplexing (Caporaso et al., 2012). Amplification reactions were performed using 12.5 uL of KAPA2G Robust HotStart ReadyMix (KAPA Biosystems), 1.5 uL of 10 uM forward and reverse primers, 7.5 uL of sterile water and 2 uL of DNA. The V4 region was amplified by cycling the reaction at 95°C for 3 minutes, 18x cycles of 95°C for 15 seconds, 50°C for 15 seconds and 72°C for 15 seconds, followed by a 5-minute 72°C extension. All amplification reactions were done in duplicate to reduce amplification bias, pooled, and checked on a 1% agarose TBE gel. Pooled duplicates were quantified using PicoGreen and combined by even concentrations.
characteristics.characteristic: description
characteristics.text: chow
characteristics.characteristic: diet
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: feces
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: Sample 7
characteristics.characteristic: title