characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-05-31T00:13:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-05-31T00:13:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10115100
characteristics.characteristic: External Id
characteristics.text: Department II of Internal Medicine and Center for Molecular Medicine Cologne, University of Cologne
characteristics.characteristic: INSDC center name
characteristics.text: 2022-05-31T00:13:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-05-31T00:13:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7771652
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10954:Sample 11
characteristics.characteristic: Submitter Id
characteristics.text: 21
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mice were sacrificed and the kidneys were excised at P21. While the right kidney was immediately frozen in liquid nitrogen, the left kidney was fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) for histology preparation. Mice were housed in a room at 22 ± 2°C and exposed to a 12-hour dark/light cycle. To induce maternal obesity female mice received a high fat diet (HFDmat; modified #C1057, Altromin, Lage, Germany) for 8-10 weeks after weaning [postnatal day 21 (P21)]. Control dams were fed standard diet (SD, Controlmat; ssniff #R/M-H, V1534-0). Both HFD and Control dams were mated with SD-fed males and continued on their respective diet throughout pregnancy and lactation. After birth, the litter size of all dams was normalized to six for each litter. Water and chow were available ad libitum. Total RNA was extracted from frozen mouse kidney using TRI Reagent (Sigma Aldrich, #T9424-200ml, St. Louis, MO, USA) according to the recommendations. The RNA concentration was measured with Tecan Infinite® M200 PRO NanoQuant and the quality of the RNA was determined by measuring a 260/280 nm-ratio. Libraries were prepared from 10 ng total RNA using QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) according to the low input/FFPE/degraded material protocol.
characteristics.characteristic: description
characteristics.text: mouse postnatal
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004390
characteristics.characteristic: developmental stage
characteristics.text: high fat diet
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002757
characteristics.characteristic: diet
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: 11
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: cortex of kidney
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001225
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000384
characteristics.characteristic: sex
characteristics.text: C57BL/6N
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004472
characteristics.characteristic: strain
characteristics.text: Sample 11
characteristics.characteristic: title