characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2019-07-11T04:02:29Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-05-04T00:19:32Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA104551716
characteristics.characteristic: External Id
characteristics.text: OREGON STATE UNIVERS
characteristics.characteristic: INSDC center name
characteristics.text: 2019-07-11T04:02:29Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-05-04T00:19:32Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS2163000
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-6422:Senescencing-shoot-minus-reproductive-parts-replicate-1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: Seeds were planted in pots containing commercial potting mix with fertilizers. Pots were covered with clear perforated plastic wrap and kept at 4 degrees celsius for 3 days to break the dormancy. After 3 days plants were transferred to the Intellus Ultra growth chamber (Percival Scientific, IA, USA) which was set to temperature 22-23 degrees celsius, light intensity 120-150 micromol/m2sec under the cycle of 16h light and 8h dark. Soil was kept moist by gently spraying with water every 72 hours to maintain humidity to 50-60%. Sampling time point is given in days after germination. Total RNA from frozen samples was extracted as a method described in Filichkin et al., 2010. Total RNA was used to isolate large RNA as per manufacturer's protocol for miRNeasy Mini kits (Qiagen Inc., USA), and RNase-free DNase (Life Technologies Inc., USA). True-Seq kit (Illumina Inc.) was used to prepare RNA-seq libraries, according to the manufacturer’s protocol.
characteristics.characteristic: description
characteristics.text: sporophyte senescent stage
characteristics.characteristic: developmental stage
characteristics.text: Col-0
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005148
characteristics.characteristic: ecotype
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: rosette
characteristics.characteristic: organism part
characteristics.text: 55
characteristics.unit: day
characteristics.characteristic: sampling time point
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: Senescencing-shoot-minus-reproductive-parts-replicate-1
characteristics.characteristic: title