characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-01T00:11:18Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-01T00:11:18Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13257492
characteristics.characteristic: External Id
characteristics.text: The Francis Crick Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-01T00:11:18Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-01T00:11:18Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10860537
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11510:pan-Pol2_R1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: 46C
characteristics.characteristic: cell line
characteristics.text: embryonic stem cell
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: The mES cells were grown in 2i+LIF medium. The cells were detached using Accutase, and fixed by formaldehyde 1.5% in PBS for 15min. To stop crosslinking glycine 125mM was added. The samples were treated with proteinase K to degrade the proteins. Then DNA was extracted using SPRI beads. ChIP-seq libraries were prepared using NEBNext Ultra II DNA library prep kit.
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: 129a mouse strain
characteristics.characteristic: strain
characteristics.text: pan-Pol2_R1
characteristics.characteristic: title