characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-01T00:11:16Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-01T00:11:16Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13130393
characteristics.characteristic: External Id
characteristics.text: Biosciences Institute, Newcastle University
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-01T00:11:16Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-01T00:11:16Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10734340
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10888_2:WSSS_F_Imm8725560
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Samples were collected by the Human Developmental Biology Resource and subsequently dissected by the Haniffa lab team. Single cells suspensions were made by mechanical digestion and live, enriched cells were selected by FACS. RNA was extracted by cell lysis during droplet encapsulation on board the 10x Chromium Controller and purified after Reverse Transcription using DynaBeads. Libraries were constructed after cDNA amplification by fragmentation and ligation of adapter sequences before a final index PCR adding sample specific barcodes and sequencing adapters.
characteristics.characteristic: description
characteristics.text: late embryonic stage
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: Carnegie stage 15
characteristics.characteristic: embryo stage
characteristics.text: F80
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: yolk sac
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: WSSS_F_Imm8725560
characteristics.characteristic: title