characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-09T08:13:20Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-09T08:13:20Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11298867
characteristics.characteristic: External Id
characteristics.text: Assistant Professor
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-09T08:13:20Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-09T08:13:20Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8947044
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11179:Sample 6
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: baker's yeast
characteristics.characteristic: common name
characteristics.text: Protocols: The yeast culture was treated and then centrifuged. The mutant yeast cells were grown overnight on SC+can media at 30 degree Celsius at 200 RPM. The cells were treated with metabolites (4NC and DP) for 3 days, and then selected for canavanine resistance on SC+can plates RNA isolation was done using TRIzol Reagent. The sample was homogenized with TRIzol Reagent, followed by addition of chloroform. The solution was allowed to separate into different layers, and the upper aqueous layer was transferred to isopropanol, in the presence of glycogen for precipitation. The precipitated RNA was washed with ethanol, and resuspended in nuclease-free water. DNAse treatment was done to remove DNA contamination. The pure RNA was again precipitated with ethanol, washed and resuspended in nuclease free water for downstream processing. SPRI Beads-based Size Selection
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: YPD media with canavanine
characteristics.characteristic: growth condition
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4932
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organism part
characteristics.text: Saccharomyces cerevisiae
characteristics.characteristic: scientific_name
characteristics.text: BY4741
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000098
characteristics.characteristic: strain
characteristics.text: 30
characteristics.unit: Celsius
characteristics.characteristic: temperature
characteristics.text: Sample 6
characteristics.characteristic: title