characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-05-06T00:32:04Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-05-06T00:32:04Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13318699
characteristics.characteristic: External Id
characteristics.text: Departments of Physiology and Medicine University of Toronto
characteristics.characteristic: INSDC center name
characteristics.text: 2022-05-06T00:32:04Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-05-06T00:32:04Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10921438
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11538:Primary L cells from regular chow fed mouse 2
characteristics.characteristic: Submitter Id
characteristics.text: 14
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: type L enteroendocrine cell
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Primary colonic L cells from Gcg-Venus mice were collected at ZT14 by fluorescence-activated cell sorting using a BD FACS Melody cell sorter (BD Bioscience) at the University of Toronto Flow Facility. Briefly, DAPI-staining as well as side- and forward-scatter were used to remove non-Venus positive and dead cells. Venus-positive cells were isolated into LoBind tubes (Eppendorf) with RLT-Buffer (Qiagen. Male proglucagon (Gcg)-Venus C57Bl/6J mice were used at 5-7-weeks of age. Throughout the duration of the experiment, mice were housed in a 12-hour light/12-hour dark cycle at constant room temperature with ad libitum access to food and water. Mice were fed a high-fat diet (HFD; 60% calories from fat; D12492, Research Diets) for 6 weeks to achieve a state of diet-induced obesity, with a sucrose-matched regular chow control diet (RC; 10% calories from fat; D12450J, Research Diets) used as control. A subset of animals in both diet groups was supplemented with 0.3% w/w nobiletin (NOB; R&S PharmChem. To avoid taste aversion, the nobiletin dose was gradually increased over the course of the first week Total RNA was extracted using the RNeasyPlus Micro Kit (Qiagen) with gDNA removal. NEBNext Ultra II Directional RNA-Seq + rRNA depletion (H/R/M)
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: regular chow
characteristics.characteristic: diet
characteristics.text: Gcg-Venus
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: colon
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: Primary L cells from regular chow fed mouse 2
characteristics.characteristic: title