characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-05-16T00:14:58Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-05-16T00:14:58Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13191460
characteristics.characteristic: External Id
characteristics.text: Karolinska Institutet Department of Cell & Molecular Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2022-05-16T00:14:58Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-05-16T00:14:58Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10794984
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11488:PBMCs 10x
characteristics.characteristic: Submitter Id
characteristics.text: 43
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: blood cell
characteristics.characteristic: cell type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: After thawing the cryoperserved PBMC sample (Lonza), we stained dead cells with propidium iodide (Thermo Fisher) and sorted 200,000 live cells into a 5 mL tube (BD FACSMelody; 100 um nozzle). After centrifugation, the cell suspension was resuspended and concentration of cells determined using a Countess automated cell counter (Thermo Fisher). We loaded ~13,000 cells for a target cell recovery of ~8,000 cells onto a 10x Genomics chip. no extraction was performed Libraries were prepared according to the 10x Genomics 3' Gene Expression v3.1 user guide.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: donor7
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: PBMCs 10x
characteristics.characteristic: title