characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-05-31T00:13:41Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-05-31T00:13:41Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13657103
characteristics.characteristic: External Id
characteristics.text: Wageningen University and Research
characteristics.characteristic: INSDC center name
characteristics.text: 2022-05-31T00:13:41Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-05-31T00:13:41Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS11258922
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11583:Sample 44
characteristics.characteristic: Submitter Id
characteristics.text: 18
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: Root systems were separated from leaves and removed from the medium. The root systems were immediately snap frozen in liquid nitrogen Plants were grown for 14 days in the 16/8 hours light/dark at 21 degrees celsius. After 14 days plants were transferred to the dark for the remaining time until sampling. Plants were grown in 6 wells plates with MS-20 medium with Gelrite. The Maxwell® 16 Tissue LEV plant RNA kit was used for mRNA isolation with a Maxwell® AS2000 instrument (Promega), following the manufacturer’s protocol. After the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) are used to isolate mRNA. Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. The suitable fragments are size-selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used in quantification and qualification of the sample library.
characteristics.characteristic: description
characteristics.text: Col-0
characteristics.characteristic: ecotype
characteristics.text: opr2-1 mutant
characteristics.characteristic: genotype
characteristics.text: Meloidogyne incognita
characteristics.characteristic: infect
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: root
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: Sample 44
characteristics.characteristic: title