characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-05-06T00:32:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-05-06T00:32:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13410840
characteristics.characteristic: External Id
characteristics.text: Departments of Physiology and Medicine University of Toronto
characteristics.characteristic: INSDC center name
characteristics.text: 2022-05-06T00:32:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-05-06T00:32:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS11012990
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11539:mGLUTag L cells treated with vehicle and vehicle - replicate 3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: mGLUTag
characteristics.characteristic: cell line
characteristics.text: type L enteroendocrine cell
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Total RNA was extracted at t=8 hr from synchronized murine GLUTag cells (reference here: 10.1007/978-3-319-16104-4_21) after treatment with vehicle or palmitate, with and without nobiletin. Cells were grown on 6-well plates in Dulbecco’s modified Eagle’s medium with 25 mmol/L glucose and 10% FBS for 48-hours. Cells were pre-treated for 12-hours with vehicle or 0.5 mM palmitate (PAL; Sigma-Aldrich) made by dissolving in water at 70°C and then adding to growth medium. Cells were also treated with vehicle or 20 μM nobiletin (R&S PharmChem and Sigma-Aldrich; dissolved in DMSO). Cells were synchronized by incubation for 12-hours in 0.5% FBS media followed by addition of 20 μM forskolin in 10% FBS media for 1-hour. Media was then changed to regular growing media with the appropriate treatments for an additional 8-hours. Total RNA was extracted using the RNeasy Plus Mini Kit with QIAshredder (Qiagen). Takara SMART-Seq v4 PLUS Kit (SSv4 PLUS)
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Gcg-SV40largeTag
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: large intestine
characteristics.characteristic: organism part
characteristics.text: neoplasm
characteristics.characteristic: sampling site
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: CD1 mus strain
characteristics.characteristic: strain
characteristics.text: mGLUTag L cells treated with vehicle and vehicle - replicate 3
characteristics.characteristic: title