characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-05-11T12:13:48Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-05-11T12:13:48Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10181905
characteristics.characteristic: External Id
characteristics.text: Institut d'Investigacio Illes Balears (IdISBa)
characteristics.characteristic: INSDC center name
characteristics.text: 2022-05-11T12:13:48Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-05-11T12:13:48Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7838360
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10977:ic-GSC2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HGCC U3035
characteristics.characteristic: cell line
characteristics.text: induced core glioma stem cell
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: ic-GSC were splited with ReLeSR™, a passaging reagent ic-GSC were cultured on coated dishes with Matrigel containing the supplemented mTeSR1 media with daily media change Lentivirus TetO-FUW-OSKM and FUW-M2rtTA were generated in 293T cells, in mTeSR1 media. The viral supernatants were mixed at 1:1 ratio and filtered. GSCs were seeded at a density of 0.3 x 106 per well of a 6-well plate, and infected four times at 8h, 24h, 32h, and 48h, with 2 μg/ml. GBM-DCs were treated with doxycycline 2g/ml to induce OSKM expression. ic-GSC colonies appeared after four weeks and single-cell clone selection were manually generated in 96-well plate. We selected three ic-GSC clones. RNA was obtained using the EZNA Total RNA Kit I Samples were processed using the TruSeq RNA Sample Prep v3 kit (Illumina) according to the manufacturers instructions.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: glioblastoma multiforme
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000519
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: ic-GSC2
characteristics.characteristic: title