characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-05-01T00:14:20Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-05-01T00:14:20Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13166507
characteristics.characteristic: External Id
characteristics.text: Section of Genetics and Genomics, Department of Metabolism, Digestion and Reproduction, Imperial College London
characteristics.characteristic: INSDC center name
characteristics.text: 2022-05-01T00:14:20Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-05-01T00:14:20Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10770086
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11475:Islets_WT3
characteristics.characteristic: Submitter Id
characteristics.text: 13
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: RNA was extracted from fresh whole liver samples. For pancreatic islets, the pancreas was inflated with an ice-cold collagenase P solution (1 mg/mL in Hanks' balanced salt solution (HBSS) buffer, Roche) injected through the main pancreatic duct. The pancreas was then dissected and incubated at 37ºC for 8 min with continuous agitation. The pancreatic tissue was disaggregated by gentle suction through a needle, and cell suspension was washed 4 times with cold HBSS-0.5% BSA. The islet pellet was resuspended in 7 mL Histopaque containing a 7:3 ratio of pre-cooled Histopaque 1077 (Merck) and Histopaque 1119 (Merck), and 7 mL of HBSS-0.5% BSA was layered on top of the Histopaque. The gradient was centrifuged at 950 g for 20 mins at RT. The interphase containing the islets was collected, washed 3 times with HBSS-0.5% BSA and islets were finally further enriched by gentle aspiration with a pipette under a stereomicroscope. Islets were allowed to recover for 2 days in culture in RPMI containing 11 mM glucose supplemented with 10% fetal calf serum and penicillin-streptomycin (1:100; Invitrogen) at 37°C and 5% CO2. RNA was prepared using RNeasy mini kit (Qiagen) including DNAse I (Qiagen) treatment. RNA-seq libraries were prepared using the Truseq Stranded mRNA Sample Prep Kit (Illumina) following manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: islet of Langerhans
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6J x PWK/PhJ
characteristics.characteristic: strain
characteristics.text: Islets_WT3
characteristics.characteristic: title