characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-05-11T12:13:48Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-05-11T12:13:48Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10181903
characteristics.characteristic: External Id
characteristics.text: Institut d'Investigacio Illes Balears (IdISBa)
characteristics.characteristic: INSDC center name
characteristics.text: 2022-05-11T12:13:48Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-05-11T12:13:48Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7838358
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10977:GBM-DC3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HGCC U3035
characteristics.characteristic: cell line
characteristics.text: glioblastoma derived cell
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: Accutase was used to split GBM-DCs. GBM-DCs were obtained from the Human Glioblastoma Cell Culture biobank (HG3035). They are cultured on dishes coated with polyornithine 10 l/ml and laminin 10 l/ml in Neurobasal and DMEM/F12 media with N2 and B27 supplements and human recombinant FGF2 and EGF 10 ng/ml RNA was obtained using the EZNA Total RNA Kit I Samples were processed using the TruSeq RNA Sample Prep v3 kit (Illumina) according to the manufacturers instructions.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: glioblastoma multiforme
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000519
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: GBM-DC3
characteristics.characteristic: title