characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-01T00:11:18Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-01T00:11:18Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA13318110
characteristics.characteristic: External Id
characteristics.text: The Francis Crick Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-01T00:11:18Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-01T00:11:18Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10920850
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11513:Input_R1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: 46C
characteristics.characteristic: cell line
characteristics.text: embryonic stem cell
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mouse embryonic stem cells (46C) were grown in 2iL medium. The cells were then harvested using Accutase, and subjected to Cut & Run assay kit provided by Cell Signaling Tech (#86652). Briefly the cells were immobilised using Concanavalin magnetic beads. Digitonin was used to permeabilize the cells. Aldh18a1 antibody (Bethyl A304-802A) or normal IgG (CST 66362) was added to samples. Protein AG-MNase was added, and activated by CaCl2. The supernatant was collected, and DNA was purified for library preparation. DNA purification was carried out using the spin columns provided by the kit. NEB Next Ultra II DNA library prep kit was used for library preparation
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: inner cell mass
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: 129a mouse strain
characteristics.characteristic: strain
characteristics.text: Input_R1
characteristics.characteristic: title