characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-05-01T00:14:10Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-05-01T00:14:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10979751
characteristics.characteristic: External Id
characteristics.text: Berlin Institute for Medical Systems Biology (BIMSB) Max Delbruck Center for Molecular Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2022-05-01T00:14:10Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-05-01T00:14:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8629572
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10902_2:PCN_mRNA_shHbs1l_Soma_2
characteristics.characteristic: Submitter Id
characteristics.text: 0
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: neuron of cerebral cortex
characteristics.characteristic: cell type
characteristics.text: neuronal cell body
characteristics.characteristic: cellular component
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Soma (neuronal cell body) samples were collected from top of the filter membrane and neurite (neuron projection) samples frpm the cleaned membranes at DIV9. See also: “Ludwik et al. 2019: Genome-wide analysis of RNA and protein localization and local translation in mESC-derived neurons” Cortical neurons were isolated from E14 and P0 pups and plated on microporous membranes in 6-well plates. Astrocytes were pre-seeded on the 6-well dishes around one week in advance of plating. Cells were cultured as previously described (Kaech & Banker, 2006). Neurons were transfected using lentiviral particles carrying either shRNA expression constructs or GFP constructs with inserts from one of the reporter library pools. Viral particles were applied onto the neurons between DIV1 and DIV5. After collection samples were immediately transferred into Trizol and RNA extraction was performed according to manufacturer’s instructions (see also Ludwik et al. 2019). 100 ng of total RNA from each sample were used for libraries preparation with either TruSeq stranded mRNA library preparation kit (Illumina 2002059), Truseq Stranded Total RNA Library kit (Illumina, 15032619) or SMART smRNaseq kit (Takara, 635029) according to the manufacturer’s recommendations.
characteristics.characteristic: description
characteristics.text: neonate
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001372
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000461
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: 4 to 8 individuals from litter10
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: brain
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000955
characteristics.characteristic: organism part
characteristics.text: mRNA
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CHEBI_33699
characteristics.characteristic: protocol
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: mixed
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001271
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: PCN_mRNA_shHbs1l_Soma_2
characteristics.characteristic: title