characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-03T00:12:41Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-03T00:12:41Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11935956
characteristics.characteristic: External Id
characteristics.text: LabMedMolGe
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-03T00:12:41Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-03T00:12:41Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS9583070
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11261:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Raw sewage samples (wastewater) were collected between December 2020 and April 2021 from different WWTPs (Waste Water Treatment Plant) located in the provinces of Naples and Salerno (Campania Region). Composite samples, representing 24-hour period were collected and stored at 4°C until use. On the arrival, samples underwent a 30 min treatment at 56 °C to increase the safety of the protocol and then concentrated with polyethylene glycol (PEG) precipitation. 45 ml of each sample were centrifuged at 4°C for 30 min at 4500g to pellet wastewater solids; then clarified wastewater was mixed with 10% PEG and 2.25% NaCl and centrifuged at 4°C for 2 h at 12000g . The pellet was finally resuspended in the appropriate volume of PBS and entirely used for RNA extraction with the 147 Total RNA purification kit (Norgen, biotel corp.), following manufacturer instructions. Indexed libraries were prepared from 12 µl of viral RNA using a targeted amplicon-based NGS panel protocol by Paragon Genomics according to manufacturer's instruction. Libraries were pooled in equimolar amounts.
characteristics.characteristic: description
characteristics.text: Nocera, Italy
characteristics.characteristic: environmental history
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: derived from sewage waste water
characteristics.characteristic: growth condition
characteristics.text: SARS coronavirus
characteristics.characteristic: organism
characteristics.text: Severe acute respiratory syndrome coronavirus
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Severe acute respiratory syndrome coronavirus
characteristics.characteristic: scientific_name
characteristics.text: 07-04-2021
characteristics.characteristic: time
characteristics.text: Sample 1
characteristics.characteristic: title