characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-14T00:18:14Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-14T00:18:14Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10422655
characteristics.characteristic: External Id
characteristics.text: ROTHAMSTED RESEARCH
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-14T00:18:14Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-14T00:18:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8075493
characteristics.characteristic: SRA accession
characteristics.text: Wolbachia_endosymbiont_of_Psylliodes chrysocephala_genome
characteristics.characteristic: Submitter Id
characteristics.text: 2017
characteristics.characteristic: collection date
characteristics.text: Wolbachia
characteristics.characteristic: common name
characteristics.text: Wolbachia endosymbiont of Psylliodes chrysocephala genome assembly. The resulting individual used for HiFi and multi-individual used for Hi-C has a parasitic wasp present (10% genome size) so I had very good coverage of the wasp and got a good assembly of that. This went to the PhD PGI linked project. However the beetle coverage was then reduced to x12 so the assembly was not as continuous as it could have been. But despite that the assembly is reasonably good, chromosome level. Freebayes route used for error correction. I suggest to improve would be to repeat using non-infected individual HiFi. Included in the data set is 10X illumina library data of non-infected individual and male and female Illumina genome resequencing too. Manual curation and Hi-C to bring to chromosome level. Single primary sequence assembly, not haplotype assembled. Maker annotation pipeline used with both transcriptome transfer and de novo gene prediction as inputs to Evigene to pick best gene models. Curation of P450, ABC, UGT, IRAC gene models where RNA-seq evidence was present. Unknown sex individual used for 10X and then male 10X and female 10X samples. Single individual with parastoid wasp taken for PacBio HiFi. Single adult beetle from field sample collected at Rothamsted farm on 28 July 2017. GPS 51.8093, -0.3548
characteristics.characteristic: description
characteristics.text: United Kingdom
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: Wolbachia endosymbiont of Psylliodes chrysocephala
characteristics.characteristic: organism
characteristics.text: Wolbachia endosymbiont of Psylliodes chrysocephala
characteristics.characteristic: scientific_name
characteristics.text: Wolbachia endosymbiont of Psylliodes chrysocephala from the Pest Genomics Initiative.
characteristics.characteristic: title