characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-17T00:17:34Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-17T00:17:34Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110001508
characteristics.characteristic: External Id
characteristics.text: MRC Cancer Unit
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-17T00:17:34Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-17T00:17:34Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12150575
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11790:CL19 H3K27ac 3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Fh1 -/- CL19
characteristics.characteristic: cell line
characteristics.text: epithelial cell
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: 1x106 Fh1 fl/fl or 2x106 Fh1 -/- cells were plated onto 15-cm dishes (Nunc) and allowed to reach 80% confluency. Cells were then washed twice in PBS and detached using trypsin (Gibco, 0.25% in PBS-EDTA). After inactivating trypsin with complete medium, cells were filtered through 70μm cell strainers (Fisher Scientific) and counted with Cell Counter CASY (OmniLife Sciences). Following an additional wash in PBS, 7x106 cells were resuspended in 500 μl of PBS and crosslinked using formaldehyde (Thermo Fisher) at 1% final concentration. Crosslinking was stopped using glycine solution after 10 min and 3 washes were carried in cold PBS before storing the pellets at -80˚C. Cells were processed using iDeal ChIP-seq kit for Histones (Diagenode, C01010051) DNA Library was generated using NEB Next Ultra II DNA library kit (NEB, E7645S) following the manufacturer’s protocol
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: hereditary leiomyomatosis and renal cell cancer
characteristics.characteristic: disease
characteristics.text: Fh1 knockout
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: kidney
characteristics.characteristic: organism part
characteristics.text: 3
characteristics.characteristic: replicate
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: CL19 H3K27ac 3
characteristics.characteristic: title