characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-13T08:16:31Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-13T08:16:31Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10632874
characteristics.characteristic: External Id
characteristics.text: The University of Zurich (UZH, Switzerland)
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-13T08:16:31Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-13T08:16:31Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8284727
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11093:J_08
characteristics.characteristic: Submitter Id
characteristics.text: 14
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: Two 3-day-old seedlings per well were transferred into transparent 24-well plates containing 1 mL liquid MS media, sealed with porous tape and grown for a further 9 days. Media was then exchanged for 500 µL fresh MS media and left overnight. In the morning a further 480 µL of fresh media was added. 9.5 h later 20 µL treatment/mock was added and seedlings were harvested after 30 min. Mock treatment was with water. All seedlings were ground in liquid nitrogen. Arabidopsis thaliana seeds grown on plates were surface sterilized using chlorine gas for 5-6 h and sown on 1/2 Murashige and Skoog (MS) media, 1 % sucrose, and 0.8 % agar and stratified at 4 °C for 2-3 days. Plates were then transferred to 22 °C under a 16-h photoperiod. Total RNA was extracted using Trizol reagent according to the manufacturer’s instructions and DNAase treatment/RNA cleanup was performed using the RNeasy kit. Library prep and sequencing were performed by Novogene using a NEB Next® Ultra™ RNA Library Prep Kit
characteristics.characteristic: description
characteristics.text: seedling development stage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007131
characteristics.characteristic: developmental stage
characteristics.text: Col-0
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005148
characteristics.characteristic: ecotype
characteristics.text: hsl3-1
characteristics.characteristic: genotype
characteristics.text: pool of two seedlings
characteristics.characteristic: individual
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3702
characteristics.characteristic: organism
characteristics.text: whole plant
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000468
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: 100 nM CTNIP4
characteristics.characteristic: stimulus
characteristics.text: J_08
characteristics.characteristic: title