characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-08-01T00:24:09Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-08-01T00:24:09Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10166711
characteristics.characteristic: External Id
characteristics.text: WASHINGTON UNIVERSITY
characteristics.characteristic: INSDC center name
characteristics.text: 2022-08-01T00:24:09Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-08-01T00:24:09Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7823186
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10984:GFP 4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mice were dissected and midline laparotomy was performed with immediate excision of ~50-100mg left hepatic lobe. This was snap frozen in liquid nitrogen within 1 minute of the time of death. Trizol Reagent nucleic acid extraction protocol was performed exactly per manufacturer specification RNA sequencing was performed by the Washington University Genome Technology Access Center (GTAC) as we reported (Y. Zhang et al., Hepatic arginase 2 (Arg2) is sufficient to convey the therapeutic metabolic effects of fasting. Nature Communications 10, 1587 (2019).) . Library preparation was performed with 10ug of total RNA with a Bioanalyzer RIN score greater than 8.0. Ribosomal RNA was removed by poly-A selection using Oligo-dT beads (mRNA Direct kit, Life Technologies). mRNA was fragmented and reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies) and random hexamers. A second strand reaction was performed to yield ds-cDNA, and then had Illumina sequencing adapters ligated to the ends.
characteristics.characteristic: description
characteristics.text: diabetes mellitus
characteristics.characteristic: disease
characteristics.text: GFP expression control
characteristics.characteristic: genetic modification
characteristics.text: db/db
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: left hepatic lobe
characteristics.characteristic: sampling site
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57 B6/J
characteristics.characteristic: strain
characteristics.text: GFP 4
characteristics.characteristic: title