characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-11T00:19:32Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-11T00:19:32Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110068838
characteristics.characteristic: External Id
characteristics.text: Sorbonne University Biologie et therapeutiques du cancer
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-11T00:19:32Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-11T00:19:32Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12167213
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11812:Cells_Slug1
characteristics.characteristic: Submitter Id
characteristics.text: 44
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: HT-29
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001193
characteristics.characteristic: cell line
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: HT-29 cells were provided by Richard Camalier (National Cancer Institute, Bethesda, MD, USA). For the genetic Slug model, HT-29 CRC cells were transfected using Lipofectamine and Plus reagent, Invitrogen. Y. Tony Ip, University of Massachusetts Medical School, Worcester, MA, USA generously provided the pCDNA3.1-SLUG plasmid encoding hu-man Slug and the control plasmid. PGL3-OPN (-213)-luc and PGL3-OPN (-1206)-luc con-taining the human proximal and distal OPN promoter, respectively, were a kind gift from Gerhart Ryffel and were provided by Addgene, Waterdown, MA, USA (plasmids # 31106 or 31107). For Tumor Xenograft, Two million cells were injected into the right flank of athymic 6 weeks old female NMRI-Fox 1nu mice (Taconic, Rensselaer, NY, USA). A total of 24 animals were used in this work, divided into 4 groups (WT, Control, Slug1, Slug2). The mice were followed for 24 days from the injection of the cells. Total RNA was extracted using a mirVana miRNA Isolation Kit from Ambion, Ther-moFisher Scientific,Waltham, MA, USA. Library preparation was performed using KAPA mRNA HyperPrep kit, Roche
characteristics.characteristic: description
characteristics.text: colorectal cancer
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005842
characteristics.characteristic: disease
characteristics.text: overexpression SNAI2/Slug (clone 1)
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: cell culture
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/BTO_0000214
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: Cells_Slug1
characteristics.characteristic: title