characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-20T00:17:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-20T00:17:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10558506
characteristics.characteristic: External Id
characteristics.text: Division of Embryology, National Institute for Basic Biology, Okazaki 444-8787, Japan Wellcome Trust–MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, Biomedical Campus, Puddicombe Way, Cambridge, CB2 0AW, UK Department of Surgery, University of Cambridge, Cambridge CB2 0QQ, UK
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-20T00:17:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-20T00:17:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8210487
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11109:RNA02.dKOB.END3.CTRL.C
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: H9
characteristics.characteristic: cell line
characteristics.text: definitive endoderm
characteristics.characteristic: cell type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were grown on 12 well plates for total RNA isolation. Three wells per condition were harvested for technical triplicates. Cell culture medum was aspirated and 250 µl of Lysis buffer in GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich) was added directly to the 12 well plates. Cell lysates were transferred to 1.5-ml tubes and were either frozen at -80C or used immediately for RNA extraction. Total RNA was extracted from cells using the GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich) and the On-Column DNase I Digestion set (Sigma-Aldrich). RNA was poly-A selected and used for library preparation with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.
characteristics.characteristic: description
characteristics.text: embryo stage
characteristics.characteristic: developmental stage
characteristics.text: shRB integrated, RBL1/2 KO
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blastocyst
characteristics.characteristic: organism part
characteristics.text: embryonic stem cell
characteristics.characteristic: progenitor cell type
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: RNA02.dKOB.END3.CTRL.C
characteristics.characteristic: title