characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-07-01T00:15:14Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-07-01T00:15:14Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110063454
characteristics.characteristic: External Id
characteristics.text: Unit of Viral Infection and Immunity, National Center for Microbiology, Institute of Health Carlos III
characteristics.characteristic: INSDC center name
characteristics.text: 2022-07-01T00:15:14Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-07-01T00:15:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12161832
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11811:31
characteristics.characteristic: Submitter Id
characteristics.text: 34
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Whole peripheral blood was isolated and processed within the first 4 h after extraction. Plasma fraction was pre-clarified at 15.000 rpm 15 min at 4°C, and stored at – 80°C. Extracellular vesicles (EVs isolation and RNA purification was performed using the ExoRNeasy Serum/Plasma Midi kit (QIAGEN, Cat #77044). EVs were phenol-lysed and total RNA was purified by ethanol-based membrane binding into spin columns. Quality and integrity were evaluated by the Bioanalyzer 2100 with Agilent RNA 6000 Nano kit (Agilent). Small RNA library was constructed with NEBNext Multiplex Small RNA Library Prep Kit (NewEngland BioLabs).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: chronic hepatitis C virus infection
characteristics.characteristic: disease
characteristics.text: extracellular vesicles
characteristics.characteristic: fraction
characteristics.text: 31
characteristics.characteristic: individual
characteristics.text: Hepatitis C virus
characteristics.characteristic: infect
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: 31
characteristics.characteristic: title