characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-29T00:16:22Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-29T00:16:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10838355
characteristics.characteristic: External Id
characteristics.text: Laboratory of Molecular Medicine and Genomics
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-29T00:16:22Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-29T00:16:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8489589
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11137:Sample 3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: U-87 MG
characteristics.characteristic: cell line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Human glioblastoma cells (U87MG) were obtained from the American Type Culture Collection (Manassas, Va.). Cells were cultured in Dulbeccos Modified Eagle Medium supplemented with 10% fetal bovine serum (Gibco Fetal Bovine Serum South America, Thermo Scientific Fisher-US), 2mM L-glutamine, 50 U/ml penicillin, 50 ?g/ml streptomycin, at 37o C, 5% CO2 and 95% of humidity U87MG glioblastoma cells have been transfected, in biological triplicate, with a siRNA targeting praja2 gene or control siRNA. Cells were collected and homogenized in Trizol Reagent. Total RNA extraction was performed with a RNA Clean and Concentrator Kit (Zymo Research Corp., Irvine, CA, USA), following the manufacturers instructions. RNA purity and integrity were assessed with a Nanodrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and a 4200 TapeStation instrument (Agilent Technologies, Santa Clara, CA, USA), respectively. For RNA purity, an A260/280 ratio of ?2.0 and a A260/230 ratio of 2.0-2.2 were considered acceptable; for RNA integrity, an RNA Integrity Number (RIN) of 9.0-10.0 has been obtained for all samples, indicating the absence of degradation and high integrity of RNA samples. For a precise estimation of the RNA concentration, a Qubit 2.0 fluorometer assay (Thermo Fisher Scientific, Waltham, MA, USA) has been employed
characteristics.characteristic: description
characteristics.text: glioblastoma multiforme
characteristics.characteristic: disease
characteristics.text: ---
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: brain
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 3
characteristics.characteristic: title