characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-07-19T20:17:27Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-07-19T20:17:27Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10105078
characteristics.characteristic: External Id
characteristics.text: Section of Paediatric Infectious Disease, Faculty of Medicine, Imperial College London
characteristics.characteristic: INSDC center name
characteristics.text: 2022-07-19T20:17:27Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-07-19T20:17:27Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7761707
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10926:Sample 51
characteristics.characteristic: Submitter Id
characteristics.text: 70
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: steroid and monoclonal antibody treatment
characteristics.characteristic: clinical history
characteristics.text: WHO severity score 5
characteristics.characteristic: clinical information
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Whole blood was collected at the time of recruitment into PAXgene blood miRNA tubes (PreAnalytiX), frozen, and total RNA (including RNA > 18 nucleotides) was isolated according to the manufacturer’s instructions (Qiagen). RNA samples were stored at −80°C, before undergoing an additional DNAse treatment using an RNA clean & concentrator kit (Zymo Research). Input material was normalised and strand specific library preparation was completed using NEBNext® Ultra™ II mRNA kit (NEB) and NEB rRNA/globin depletion probes following manufacturer’s instructions. Libraries were on a Tetrad (Bio-Rad) using in-house unique dual indexing primers. Individual libraries were normalised using Qubit and pooled together. The pooled library was diluted to ~10 nM for storage and denatured and further diluted prior to loading on the sequencer.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: COVID-19
characteristics.characteristic: disease
characteristics.text: severe disease severity
characteristics.characteristic: disease staging
characteristics.text: P025
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: Sample 51
characteristics.characteristic: title