characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-08-01T00:24:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-08-01T00:24:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10378426
characteristics.characteristic: External Id
characteristics.text: Hagai Lab The Shmunis School of Biomedicine and Cancer Research Faculty of Life Sciences Tel Aviv University
characteristics.characteristic: INSDC center name
characteristics.text: 2022-08-01T00:24:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-08-01T00:24:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8033000
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11056:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: fibroblast
characteristics.characteristic: cell type
characteristics.text: Protocols: Cells were washed with PBS and then trypsinized. Human and Rousettus samples of the same treatment condition (poly(I:C) resp. lipofectamine) were pooled together. Cells were stimulated with 1 ug/mL of High-Molecular Weight poly(I:C) (InvivoGen, Cat. Code: tlrl-pic), transfected with 2 uL/mL Lipofectamin 2000 (Thermo Fisher Scientific), for 4 hours before collection. 7000 live, single cells were loaded on to each of the Chromium Controllers (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit (version 3.1) as per the 10x Genomics protocol.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: mixed sample
characteristics.characteristic: organism
characteristics.text: mixed sample
characteristics.characteristic: organism
characteristics.text: dermis
characteristics.characteristic: organism part
characteristics.text: mixed sample
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: Rousettus aegyptiacus; Homo sapiens
characteristics.characteristic: species mix
characteristics.text: poly(I:C)
characteristics.characteristic: stimulus
characteristics.text: 4
characteristics.unit: hour
characteristics.characteristic: time
characteristics.text: Sample 1
characteristics.characteristic: title