characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-13T00:14:56Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-13T00:14:56Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110055411
characteristics.characteristic: External Id
characteristics.text: Roche Pharmaceutical Research and Early Development (pRED) Roche Innovation Center Basel
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-13T00:14:56Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-13T00:14:56Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12157511
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11773:20210628_Sample_5
characteristics.characteristic: Submitter Id
characteristics.text: 1
characteristics.characteristic: batch
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: CD8-positive, alpha-beta T cell
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/CL_0000907
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Tumour were digested as previously described and 1-10 Mio cells were stored in liquid nitrogen in Ibidi freezing medium. The samples were randomised and processed in 2 different batches with 10 samples each. After thawing a batch of samples, the cells were stained with a mix of FACS and oligo-labelled antibodies and sorted for CD8+ T cells before performing scRNA-seq. The cell number and viability of the sorted cells was determined using a Nexcelom Cellometer Auto 2000 and a total of 10,000 viable cells per sample were loaded into the 10x Genomics Chromium controller. cDNA and library preparation was performed according to the manufacturer’s indications (scRNA-seq 5’ v2 kit with TCR and feature barcoding).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: pancreatic cancer
characteristics.characteristic: disease
characteristics.text: Panc02-Fluc
characteristics.characteristic: genotype
characteristics.text: E1
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: pancreas
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0001264
characteristics.characteristic: organism part
characteristics.text: neoplasm
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000616
characteristics.characteristic: sampling site
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0000383
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0000606
characteristics.characteristic: strain
characteristics.text: 20210628_Sample_5
characteristics.characteristic: title