characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-06-15T12:17:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-06-15T12:17:01Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10379574
characteristics.characteristic: External Id
characteristics.text: INSERM Gustave Roussy institute
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-15T12:17:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-06-15T12:17:01Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8033022
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11044:S8_3_S5
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0009737
characteristics.characteristic: broker name
characteristics.text: CD34-negative, CD41-positive, CD42-positive megakaryocyte cell
characteristics.characteristic: cell type
characteristics.text: human
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: common name
characteristics.text: Protocols: The CD41+/CD42+ MK were sorted (10 000 to 30 000 cells). Cells were submitted to cell lysis, transposition, and purification steps according to Illumina protocol. The transposed DNA fragments were amplified by PCR 12 times using adapters from the Nextera Index Kit (Illumina). PCR purification was performed using MinElute PCR Purification Kit (Qiagen, 28004) to remove large fragments and remaining primers. Library quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies 5067-4626).
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: Down syndrome
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001064
characteristics.characteristic: disease
characteristics.text: GATA1s mutation, MPL mutation and SMC3 mutation
characteristics.characteristic: genotype
characteristics.text: trisomy
characteristics.characteristic: karyotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: embryo
characteristics.characteristic: organism part
characteristics.text: induced pluripotent stem cell
characteristics.characteristic: progenitor cell type
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: S8_3_S5
characteristics.characteristic: title