ERC000011
ENA-CHECKLIST
2022-08-10T16:27:35Z
ENA-FIRST-PUBLIC
2022-08-10T16:27:35Z
ENA-LAST-UPDATE
SAMEA110586937
External Id
Research Institute for Farm Animal Biology (FBN) Institute of Genome Biology
INSDC center name
2022-08-10T16:27:35Z
INSDC first public
2022-08-10T16:27:35Z
INSDC last update
public
INSDC status
ERS12684719
SRA accession
E-MTAB-12063:802
Submitter Id
70
day
age
ArrayExpress
broker name
house mouse
common name
Protocols: Tissue samples were removed, weighed, shock frozen in liquid nitrogen, and stored at -70°C. Total RNA was isolated using TRI reagent (Sigma-Aldrich, Taufkirchen, Germany). DNA traces were removed by DNaseI-treatment (Baseline-Zero DNase; Biozym, Hessisch Oldendorf, Germany), and the RNA was cleaned-up using the RNA Clean&Concentrator-25 Kit (Zymo Research, Freiburg, Germany). RNA quantity was determined using a Qubit fluorometer (ThermoFisher Scientific, Germany), and integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). 1 µg of total RNA with RIN > 8 was used for library preparation using a TruSeq Stranded mRNA Kit with 11 cycles of PCR-amplification according to the manufacture’s recommendation (Illumina).
description
adult
developmental stage
normal
disease
wild type genotype
genotype
Mus musculus
organism
Mus musculus
organism
pituitary gland
organism part
Mus musculus
scientific_name
male
sex
DUhTP
strain
802
title