ERC000011
ENA-CHECKLIST
2022-08-18T04:15:53Z
ENA-FIRST-PUBLIC
2022-08-18T04:15:53Z
ENA-LAST-UPDATE
SAMEA110668950
External Id
The University of Western Australia; Ear Science Institute Australia
INSDC center name
2022-08-18T04:15:53Z
INSDC first public
2022-08-18T04:15:53Z
INSDC last update
public
INSDC status
ERS12766529
SRA accession
E-MTAB-12102:A9
Submitter Id
ArrayExpress
broker name
adipose tissue derived mesenchymal stem cell
cell_type
Norway rat
common name
Protocols: After incubation, cells were rinsed in PBS twice before being lysed directly in Buffer RLT Plus from the RNeasy plus micro kit (Qiagen, Victoria, Australia). Cells were cultured in a humidified incubator at 37ºC, with 5% carbon dioxide. Growth medium provided contains 1 g/L D-glucose (Gibco, Victoria, Australia) Dulbecco’s Modified Eagle Medium (DMEM), L-Glutamine and 110 mg/L Sodium pyruvate, supplemented with 10% foetal bovine serum (Bovogen, Victoria, Australia) and 1% Penicillin-Streptomycin (Gibco, Victoria, Australia). Total RNA was extracted from lysed cells using the RNeasy Plus Micro kit (Qiagen, Victoria, Australia). Quality of RNA was determined on the 2100 BioAnalyzer (Agilent Technologies, Victoria, Australia). All samples of at least an RNA Integrity Number (RIN) of ≥ 8.0 and an A260/280 ratio of 1.8 - 2.0 were accepted. Library preparation was performed on 1 µg total RNA per sample with the Illumina TruSeq® Stranded mRNA kit according to manufacturer’s protocol (Illumina, San Diego, California).
description
Rattus norvegicus
organism
Rattus norvegicus
organism
adipose tissue
organism part
3
replicate
Rattus norvegicus
scientific_name
A9
title