ERC000011
ENA-CHECKLIST
2022-08-17T00:15:15Z
ENA-FIRST-PUBLIC
2022-08-17T00:15:15Z
ENA-LAST-UPDATE
SAMEA110471498
External Id
GENENTECH
INSDC center name
2022-08-17T00:15:15Z
INSDC first public
2022-08-17T00:15:15Z
INSDC last update
public
INSDC status
ERS12569521
SRA accession
E-MTAB-12036:Day_21_DTR_neg
Submitter Id
ArrayExpress
broker name
fibroblast
cell_type
house mouse
common name
Protocols: Tumors were collected, weighed and minced into small pieces. To isolate naive flank skin, hair was shaved, adipose tissue was removed and skin tissue was minced. All tissues were subsequently enzymatically digested using a cocktail of dispase (Life Technologies, Carlsbad, CA), collagenase P and DNaseI (Roche, Penzberg, Germany) for 45 min at 37°C, to obtain a single cell suspension. Cells were counted using a Vi-CELL XR (Beckman Coulter, Brea, CA). For cytokine staining, cell suspensions were stimulated with eBioscience Cell Stimulation Cocktail plus protein transport inhibitors (cat. 00-4975-93) resuspended in RPMI with 10% FBS plus 2 mmol/L l-glutamine and 2-Mercaptoethanol for 2 hours at 37°C.Cells were labelled with monoclonal antibodies purchased from BioLegend. Samples were enriched for CD24-CD45- stromal cells. Sorted cells were loaded onto a 10x Chromium Chip using reagents from the Chromium Single Cell 3’ Library Kit (10x Genomics) according to the manufacturer’s protocol. Amplified cDNA was used for 3’ RNA-seq library generation, RNA-seq libraries were prepared following the manufacturer’s user guide (10x Genomics).
description
adult
developmental stage
cancer
disease
wild type genotype
genotype
injected subcutaneous with KPR cells
growth condition
CD24-CD45-
immunophenotype
Mus musculus
organism
Mus musculus
organism
skin of body
organism part
Mus musculus
scientific_name
Day_21_DTR_neg
title