ERC000011
ENA-CHECKLIST
2022-08-08T14:39:08Z
ENA-FIRST-PUBLIC
2022-08-08T14:39:08Z
ENA-LAST-UPDATE
SAMEA110472419
External Id
Zhongkai University of Agriculture and Engineering
INSDC center name
2022-08-08T14:39:08Z
INSDC first public
2022-08-08T14:39:08Z
INSDC last update
public
INSDC status
ERS12570439
SRA accession
E-MTAB-12057:EG3
Submitter Id
ArrayExpress
broker name
grass carp
common name
Protocols: The fish were unsystematically divided into three groups of 270 fish with triplicates. The corresponding fish dietary treatments were prepared using a basic feed (purchased from Tongwei Group, Chengdu, China) for the control group (CG) and the same but supplemented with enrofloxacin (EG) or florfenicol (FG) at a concentration of 10 mg/kg.The midgut tissues of 12 individual fish from each of the three treatment groups were snap-frozen and aseptically harvested in liquid nitrogen for their microbial analyses. The grass carp midguts were aseptically dissected and collected from the control, enrofloxacin, and florfenicol groups (respectively, n = 12 fish per tank). Total genomic DNA was isolated from the midgut samples with a DNeasy PowerSoil kit (Qiagen, Hilden, Germany) according its manufacturer’s instructions. The V3-V4 hypervariable region was amplified by a universal pair of primers (343F-798R).Sequencing libraries were created by blending the amplicons and then assembling them on Illumina MiSeq platform PE300.
description
Ctenopharyngodon idella
organism
Ctenopharyngodon idella
organism
gut
organism part
Ctenopharyngodon idella
scientific_name
enrofloxacin
stimulus
EG3
title