ERC000011
ENA-CHECKLIST
2022-08-08T16:39:05Z
ENA-FIRST-PUBLIC
2022-08-08T16:39:05Z
ENA-LAST-UPDATE
SAMEA110472428
External Id
Zhongkai University of Agriculture and Engineering
INSDC center name
2022-08-08T16:39:05Z
INSDC first public
2022-08-08T16:39:05Z
INSDC last update
public
INSDC status
ERS12570448
SRA accession
E-MTAB-12058:EG2
Submitter Id
0.5
year
age
ArrayExpress
broker name
grass carp
common name
Protocols: The fish were unsystematically divided into three groups of 270 fish with triplicates. The corresponding fish dietary treatments were prepared using a basic feed (purchased from Tongwei Group, Chengdu, China) for the control group (CG) and the same but supplemented with enrofloxacin (EG) or florfenicol (FG) at a concentration of 10 mg/kg.The midgut tissues of 12 individual fish from each of the three treatment groups were snap-frozen and aseptically harvested in liquid nitrogen for the transcriptomic analyses. Total RNA was extracted from the midgut of grass carp by using the TRlzol Reagent and following the manufacturer’s instructions (Life Technologies, California, USA). cDNA libraries were constructed as per the instruction manual of NEBNext Ultra RNA Library Prep Kit.
description
Ctenopharyngodon idella
organism
Ctenopharyngodon idella
organism
gut
organism part
Ctenopharyngodon idella
scientific_name
enrofloxacin
stimulus
EG2
title