ERC000011
ENA-CHECKLIST
2022-08-08T16:44:15Z
ENA-FIRST-PUBLIC
2022-08-08T16:44:15Z
ENA-LAST-UPDATE
SAMEA110452542
External Id
Sun Yat-sun University Zhongshan Ophathlamic Center
INSDC center name
2022-08-08T16:44:15Z
INSDC first public
2022-08-08T16:44:15Z
INSDC last update
public
INSDC status
ERS12550572
SRA accession
E-MTAB-102251659426996:FMHippo_1211-H3-2
Submitter Id
5
year
age
ArrayExpress
broker name
crab-eating macaque
common name
Protocols: Total 8 monkeys (4-15 years old, 8 males and 2 females, Macaca fascicularis) were used in the current project. All procedures complied with the NIH Guide for the Care and Use of Laboratory Animals approved by Sun Yat-sen University. Following 2 weeks of adaptive housing in the institutional animal facility, animals were anesthetized with a lethal dose of sodium pentobarbital. Animals were transcardially perfused with 2 liters of artificial cerebrospinal fluid (ACSF, in mM: 125 NaCl, 25 NaHCO3, 1 NaH2PO4, 2 KCl, 25 D-Glucose, 2 CaCl2, 1 MgCl2, 5 sodium l-ascorbate, 3 sodium pyruvate, 0.01 taurine, 2 thiourea, 1 kynurenic acid, 0.1 DL-AP5, and 1x10-3 tetrodotoxin). The flow of perfusion fluid was adjusted so that the perfusion finished within approximately 40 minutes. The brain was removed from the skull immediately and a block of tissue containing the hippocampus was cut off. The position of hippocampus referred to the atlas of the rhesus monkey brain (Saleem and Logothetis, 2012). The tissue block was kept in the ACSF and sectioned on a vibratome (VT1200S, Leica) in 300 um thick slices. The adherent tissues around the hippocampus of each slice were removed on ice and only the regions of interest were kept for dissociation. 10X single cell isolation protocol v2 10X Genomics Chromium platform v2
description
adult
developmental stage
normal
disease
wild type genotype
genotype
FM_1211
individual
Macaca fascicularis
organism
Macaca fascicularis
organism
brain
organism part
hippocampus
sampling site
Macaca fascicularis
scientific_name
male
sex
FMHippo_1211-H3-2
title