characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-09-22T00:41:13Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-09-22T00:41:13Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110424412
characteristics.characteristic: External Id
characteristics.text: LUMC
characteristics.characteristic: INSDC center name
characteristics.text: 2022-09-22T00:41:13Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-09-22T00:41:13Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12522776
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11971:SV-FLB_6_AAVS1-BAC_WGS
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: LUMCi028-A
characteristics.characteristic: cell_line
characteristics.text: induced pluripotent stem cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Approximately 1x10^6 hiPSCs (cell line described here: https://hpscreg.eu/cell-line/LUMCi028-A) were rinsed once with PBS without Mg2+ and Ca2+ (PBS-/-) and dissociated to single cells with 1x TrypLE Select (ThermoFisher). The dissociation reagent was neutralized with culture medium (DMEM/F12, BSA 0.5%) and the pellet of cells was resuspended in PBS-/- and kept at -20°C until the genomic DNA extraction protocol was performed. Genomic DNA was extracted from hiPSCs using the High Pure PCR Template Preparation Kit (Roche) and treated with RNAse A (10 mg/ml, Invitrogen) according to the manufacturers’ instructions. The nucleic acid library was constructed using the NEBNext® Ultra II FS DNA and Ligation kits to fragment, A-tail and ligate sequencing adapters to the genomic DNA. The size of the resulting product was approximately 500-700 bp.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: NC_000019.10:g.55115766_55115767ins[N[?];NC_000007.14:g.150848452_151010445;N[?]]
characteristics.characteristic: genetic modification
characteristics.text: genetically modified genotype
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: skin
characteristics.characteristic: organism part
characteristics.text: fibroblast
characteristics.characteristic: progenitor cell type
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: SV-FLB_6_AAVS1-BAC_WGS
characteristics.characteristic: title