characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-09-16T00:20:06Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-09-16T00:20:06Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110587066
characteristics.characteristic: External Id
characteristics.text: Institute of Life Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2022-09-16T00:20:06Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-09-16T00:20:06Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12684847
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12067:HT-29_ crispr IRGM_Infected (Replicate-3)
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HT-29
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: HT-29 control, crispr IRGM and crispr IRGM and RIPK2 KD , mock and infected cells were collected from 60mm dish RNA was extracted from the cells(three biological replicates) using RNeasy mini kit (QIAGEN, #74104). The quality and quantity of total RNA was checked using agarose gel and Qubit 3.0 After assessing the quality of RNA, approx. 850 ng of total RNA was taken for library preparation using NEBNext 758 ®Ultra™ II Directional RNA Library kit for Illumina ® (# E7760L) and NEBNext 759 ® Poly (A) mRNA Magnetic 760 Isolation Module (# E7490L) as per manufacturer's protocol.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: colorectal adenocarcinoma
characteristics.characteristic: disease
characteristics.text: CRISPR/Cas9 mediated knockdown of IRGM
characteristics.characteristic: genotype
characteristics.text: Shigella flexneri
characteristics.characteristic: infect
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: colon
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: HT-29_ crispr IRGM_Infected (Replicate-3)
characteristics.characteristic: title