characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-08-30T00:12:29Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-08-30T00:12:29Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10157784
characteristics.characteristic: External Id
characteristics.text: Institute of Botany, the Chinese Academy of Sciences, Beijing
characteristics.characteristic: INSDC center name
characteristics.text: 2022-08-30T00:12:29Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-08-30T00:12:29Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7814271
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10957:col_2
characteristics.characteristic: Submitter Id
characteristics.text: 2
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: Total RNA was isolated from two-week-old wild-type and atnusg mutant young leaves. For total RNA isolation, frozen sample was ground in liquid nitrogen, and total RNA were extracted using Trizol reagent plus RNeasy mini kit,according to the manufacturer’s instructions mRNA was enriched using oligo-(dT) primer for nuclear-encoded genes and reverse-transcribed using random hexamers primers. The double-stranded cDNA was purified using AMPure XP beads and treated with End Prep Enzyme Mix to repair both ends and to add a dA-tail in one reaction, followed by T-A ligation to add adaptors to both ends. Size selection of adaptor-ligated DNA was performed using AMPure XP beads, and ~360-bp fragments (with an approximate insert size of 300 bp) were recovered. The library was then constructed and sequenced using an Illumina platform
characteristics.characteristic: description
characteristics.text: seedling developmental stage
characteristics.characteristic: developmental stage
characteristics.text: Col-0
characteristics.characteristic: ecotype
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: col_2
characteristics.characteristic: title