characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-09-01T00:13:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-09-01T00:13:47Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10289704
characteristics.characteristic: External Id
characteristics.text: Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland
characteristics.characteristic: INSDC center name
characteristics.text: 2022-09-01T00:13:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-09-01T00:13:47Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7944507
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11028:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: 8 to 12
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: BALB/c mice were injected with CFZ (16 mg/kg, i.v.), CFZ and atRA (CFZ: 16 mg/kg i.v., atRA: 1 mg/kg i.v.) or Captisol (vehicle, i.v.) (2 mice per cohort). ECGs were recorded under anesthesia at the end of 1h treatment and the mice were sacrificed by cervical dislocation. Euthanized animals were perfused with 20 ml of PBS and small heart tissue pieces were digested three times for 20 min at 37°C with 120 µg/ml collagenase P (Roche), 25 µg/ml DNase I (Applichem, Germany) in medium (RPMI-1640 containing 5% FCS (Sigma-Aldrich, Switzerland) and10 mM HEPES (Sigma-Aldrich, Switzerland). To eliminate remaining erythrocytes, the cell suspensions were incubated with MACS anti-Ter119 microbeads (Miltenyi Biotec, Germany) and passed through a MACS LS column (Miltenyi Biotec, Germany). Unbound single cell suspensions were stained with Live/Dead Fixable e780 Aqua staining. Live cells were sorted with a BD FACS Melody cell sorter (BD Biosciences, CA, USA). cDNA library generation was performed following the established commercial protocol for Chromium Single Cell 3’ Reagent Kit (v3 Chemistry).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: mouse 1
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: heart
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: Balb/c
characteristics.characteristic: strain
characteristics.text: Sample 1
characteristics.characteristic: title