characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-09-01T00:14:21Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-09-01T00:14:21Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110364475
characteristics.characteristic: External Id
characteristics.text: UOE
characteristics.characteristic: INSDC center name
characteristics.text: 2022-09-01T00:14:21Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-09-01T00:14:21Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12462672
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-9917_2:030-2P-1
characteristics.characteristic: Submitter Id
characteristics.text: 68
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Surgical specimens were collected and either (i) snap-frozen and stored at -70oC or (ii) fixed in formalin and embedded in paraffin. For fresh frozen tissues, RNA and DNA were isolated using QIAGEN RNAeasy and DNAeasy kits, respectively, according to the manufacturer’s protocols. For FFPE tissues, RNA and DNA were isolated with QIAGEN AllPrep FFPE Kit, according to the manufacturer’s protocols. RNA quality was assessed with an Agilent BioAnalyzer RNA 6000 Nano Kit. Fresh-frozen RNA ribo-zero library was prepared with rRNA removed from total RNA using Epicentre’s Ribo-Zero rRNA Removal kit (Cat# RZH11042). 30-100 ng Ribo-Zero RNA was used for the construction of the library using the Illumina TruSeq™ RNA Sample Prep Kit (Cat# RS-122-2001) and the manufacturer’s instructions were followed, except for omitting the purification step before fragmentation. FFPE RNA was prepared with the Illumina TruSeq FFPE RiboZero Gold protocol according to the manufacturer’s instructions.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: estrogen-receptor positive breast cancer
characteristics.characteristic: disease
characteristics.text: Patient 030
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: mammary gland
characteristics.characteristic: organism part
characteristics.text: neoplasm
characteristics.characteristic: sampling site
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: FFPE specimen
characteristics.characteristic: specimen with known storage state
characteristics.text: 030-2P-1
characteristics.characteristic: title