characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-09-11T00:15:53Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-09-11T00:15:53Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110643049
characteristics.characteristic: External Id
characteristics.text: WIS
characteristics.characteristic: INSDC center name
characteristics.text: 2022-09-11T00:15:53Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-09-11T00:15:53Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12740814
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12037:Sample 20
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: OCI-AML-3
characteristics.characteristic: cell_line
characteristics.text: blood cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: cells were spun at 1000g for 5 min. Cells pellets were mixed with 50 ul of 50 mM NaOH and heated at 99 °C for 10 min. Then, the reactions were cooled down at room temperature, and 5 ul 1 M Tris PH = 8 was added to each reaction. cells were spun at 1000g for 5 min. Cells pellets were mixed with 50 ul of 50 mM NaOH and heated at 99 °C for 10 min. Then, the reactions were cooled down at room temperature, and 5 ul 1 M Tris PH = 8 was added to each reaction. Dual indexed Illumina Libraries were generated using a two-step PCR procedure. First PCR primer prefix sequences and second PCR primer sequences were used, similar to a previously described method. First PCR was applied to target the regions of interest. The reaction mixture was composed of a PCR-ready mix (using NEBNext® Ultra™ II Q5® Master Mix, NEB, M0544L), a cell lysis product, and a final primer concentration of 1uM, for each primer. 2nd PCR was performed using the Illumina sequencing primers, indexes, and adapters, under similar conditions to the 1st PCR, except for the final primer concentration of 0.5uM for each primer, and the number of amplification cycles (n=20)
characteristics.characteristic: description
characteristics.text: acute myeloid leukemia
characteristics.characteristic: disease
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: DSB_20
characteristics.characteristic: sample id
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 20
characteristics.characteristic: title