characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-09-15T00:14:43Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-09-15T00:14:43Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110188217
characteristics.characteristic: External Id
characteristics.text: Faculty of Biology - Technion Israel Institute of Technology
characteristics.characteristic: INSDC center name
characteristics.text: 2022-09-15T00:14:43Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-09-15T00:14:43Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12287021
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11877:ctrl_VP16_B
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HCT116
characteristics.characteristic: cell line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: HCT116 cells were plated at 6cm cells and 24 hours later transfected with control or RBM42 siRNA. 48 hours after siRNA transfection, cells were treated with 20 micromolar VP16 (etoposide) for 12 hours. Cells were directly lysed using TRIzol reagent (Ambion) for RNA extraction. HCT116 cells were grown in a humidified 37C incubator (5% CO2) in RPMI-1640 medium (Gibco) supplemented with 10% heat-inactivated FBS, 2mM L-glutamine (Gibco), 100unit/mL penicillin and 100μg/mL streptomycin (Gibco). siRNA transfections were performed using Lipofectamine™ RNAiMAX (Invitrogen) according to the manufacturer's instructions. Total RNA was extracted from cells using TRIzol reagent according to the manufacturer's instructions (Ambion). RNA-seq libraries were prepared at the Crown Genomics institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science using TruSeq mRNA library preparation kit (Illumina). Libraries were quantified by Qubit (Thermo fisher scientific) and TapeStation (Agilent).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: colorectal carcinoma
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: colon
characteristics.characteristic: organism part
characteristics.text: 20 micromolar VP16 (etoposide) - 12 hours
characteristics.characteristic: response to treatment
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: ctrl_VP16_B
characteristics.characteristic: title