characteristics.text: ERC000020
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-09-01T00:18:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-09-01T00:18:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110398766
characteristics.characteristic: External Id
characteristics.text: DUNDEE
characteristics.characteristic: INSDC center name
characteristics.text: 2022-09-01T00:18:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-09-01T00:18:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12496789
characteristics.characteristic: SRA accession
characteristics.text: AC56
characteristics.characteristic: Submitter Id
characteristics.text: rural area [ENVO:01000772]
characteristics.characteristic: broad-scale environmental context
characteristics.text: 2015-02-16
characteristics.characteristic: collection date
characteristics.text: Barley rhizosphere DNA from sample grown in soil conditioned with Morex (domesticated) barley under nitrogen limiting conditions followed by heat treatment at 126°C, repeated twice at an interval of 12 hours. The soil was then used as a substrate for growth of Morex (domesticated) barley with a 25%N solution
characteristics.characteristic: description
characteristics.text: farm soil [ENVO:00005749]
characteristics.characteristic: environmental medium
characteristics.text: United Kingdom
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 56.45139
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: -3.074722
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: Barley
characteristics.characteristic: host common name
characteristics.text: 4513
characteristics.characteristic: host taxid
characteristics.text: research station [ENVO:00003919]
characteristics.characteristic: local environmental context
characteristics.text: Morex (domesticated) barley plants were grown in a 0% N solution (0mM 2(Nh4)SO4; 0mM Ca(NO3)2; 0mM KNO3; 3mM MgSO4; 100µM FeEDTA+; 1mM KH2PO4; 0.025mM CaSO4+2H20; 0.08mM CaCl2; 0.25mM KCl) until early stem elongation, following which plants were removed. The soil was then heat treated at 126°C twice at an interval of 12 hours. Morex (domesticated) barley plants were then grown in the soil with a 25% N solution (6.25mM 2(Nh4)SO4; 10mM Ca(NO3)2; 2.5mM KNO3; 3mM MgSO4; 100µM FeEDTA+; 1mM KH2PO4; 0.019mM CaSO4+2H20; 0.06mM CaCl2; 0.19mM KCl)
characteristics.characteristic: mineral nutrient regimen
characteristics.text: Hordeum vulgare
characteristics.characteristic: organism
characteristics.text: 515F: GTGCCAGCMGCCGCGGTAA; 806R: GGACTACHVGGGTWTCTAAT
characteristics.characteristic: pcr primers
characteristics.text: Barley NT
characteristics.characteristic: project name
characteristics.text: Hordeum vulgare
characteristics.characteristic: scientific_name
characteristics.text: MiSeq [OBI:0002003]
characteristics.characteristic: sequencing method
characteristics.text: AC56
characteristics.characteristic: source material identifiers
characteristics.text: Morex/Morex
characteristics.characteristic: subspecific genetic lineage
characteristics.text: 16S rRNA
characteristics.characteristic: target gene
characteristics.text: V4
characteristics.characteristic: target subfragment
characteristics.text: Barley Rhizosphere sample AC56
characteristics.characteristic: title