characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-09-01T00:13:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-09-01T00:13:47Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10289506
characteristics.characteristic: External Id
characteristics.text: Department of Biochemistry University of Cambridge
characteristics.characteristic: INSDC center name
characteristics.text: 2022-09-01T00:13:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-09-01T00:13:47Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7944341
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11024:mck1_13h_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: 2X10(7)exponentially growing (5h post-inoculation) and early post-diauxic phase (13h post-inoculation) cells were harvested by centrifugation at 1000 x g for 5 min at 4°C. Total RNA was isolated from using an RNeasy Mini kit (Qiagen, 74104). After the QC procedures of total RNA, mRNA is enriched using oligo(dT) beads. Enriched mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is first synthesized by using random hexamers primer, after which a custom second-strand synthesis buffer (Illumina), dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.
characteristics.characteristic: description
characteristics.text: mck1 deletion
characteristics.characteristic: genotype
characteristics.text: glucose-starved (early post diauxic shift)
characteristics.characteristic: growth condition
characteristics.text: early post-diauxic phase
characteristics.characteristic: growth stage
characteristics.text: Saccharomyces cerevisiae S288C
characteristics.characteristic: organism
characteristics.text: Saccharomyces cerevisiae S288C
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: 2
characteristics.characteristic: replicate
characteristics.text: Saccharomyces cerevisiae S288C
characteristics.characteristic: scientific_name
characteristics.text: BY4742
characteristics.characteristic: strain
characteristics.text: mck1_13h_2
characteristics.characteristic: title