characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-09-10T04:26:32Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-09-10T04:26:32Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11024905
characteristics.characteristic: External Id
characteristics.text: STANFORD UNIVERSITY
characteristics.characteristic: INSDC center name
characteristics.text: 2022-09-10T04:26:32Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-09-10T04:26:32Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8674660
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11158:Sample 3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: eHAP
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: >1000-fold coverage of mutagenized cells (Brunello library) was infected with Reovirus Type 3 Dearing Strain (MOI=0.1 PFU/cell). When resistant colonies of cells were clearly apparent, the cells were harvested. Uninfected starting populations of mutagenized cells were used as the unselected reference. Total genomic DNA from both virus-resistant and uninfected cells was respectively extracted using QIAamp DNA Mini Kit (Qiagen). The inserted guide RNA sequences were retrieved from the genomic DNA by PCR amplification.
characteristics.characteristic: description
characteristics.text: chronic myeloid leukemia
characteristics.characteristic: disease
characteristics.text: CRISPR knockout by Brunello Library (PMID: 26780180)
characteristics.characteristic: genetic modification
characteristics.text: lentiCas9-Blast; lentiGuide-Puro
characteristics.characteristic: genotype
characteristics.text: uninfected/unselected control
characteristics.characteristic: infect
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: expressing Cas9
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 3
characteristics.characteristic: title