characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-08-31T00:12:58Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-08-31T00:12:58Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10083232
characteristics.characteristic: External Id
characteristics.text: IFEVA (CONICET-UBA), Facultad de Agronomia (FAUBA), Universidad de Buenos Aires (UBA)
characteristics.characteristic: INSDC center name
characteristics.text: 2022-08-31T00:12:58Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-08-31T00:12:58Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7739892
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10925:Sample_15
characteristics.characteristic: Submitter Id
characteristics.text: 12
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Col-0
characteristics.characteristic: cultivar
characteristics.text: Protocols: At least 50 seedlings were pooled from three independent petri dishes for RNA extraction. Arabidopsis thaliana seeds were surfaced sterilized and sown in petri dishes with agarified (0.8% Bacto Agar) 0.5x MS medium, containing 1% sucrose and 0.05% MES at pH 5.7. After stratification for 3 days at 4°C, seedlings were grown in a CU-22L growth chamber (Percival) under 21°C and a 12/12 h (day/night) light cycle. For treatment, 7 days-old seedlings were subjected to changes in temperature only during the light period. Long-term heat consisted of an entrainment treatment of 5 heat cycles (day 37C, night 21C, 12/12 h; age at harvest 12 days). Controls were kept at 21C (age at harvest 12 days). Total RNA was isolated from biologically duplicated samples using the the Plant-RNAeasy kit (Invitrogen), following provider's instructions. Libraries were prepared with the corresponding Illumina library preparation kit, following provider’s instructions.
characteristics.characteristic: description
characteristics.text: seedling development stage
characteristics.characteristic: developmental stage
characteristics.text: drm2-2; cmt3-11
characteristics.characteristic: genotype
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: Sample_15
characteristics.characteristic: title