characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-09-13T00:15:45Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-09-13T00:15:45Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10241697
characteristics.characteristic: External Id
characteristics.text: Helmholtz Center Munich
characteristics.characteristic: INSDC center name
characteristics.text: 2022-09-13T00:15:45Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-09-13T00:15:45Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7898024
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11001:Shoot_N_salt_ctrl_rep2
characteristics.characteristic: Submitter Id
characteristics.text: 2
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: date palm
characteristics.characteristic: common name
characteristics.text: Nabut Saif
characteristics.characteristic: cultivar
characteristics.text: Protocols: Harvested plant material of both experiments (total leaves, whole roots) was immediately cut into small pieces, homogenized in liquid nitrogen to fine powder and stored at -80°C until further analyses. About two years old seedlings of micro-propagated Phoenix dactylifera, cv Khalas and Nabut Saif were purchased from Date Palm Developments Ltd. (Sommerset, U.K.). They were directly planted in 5 L pots filled with 70% quartz gravel (3 - 5mm diameter, Quarzwerke GmbH, Frechen, Germany) and covered by 3 - 5 cm soil substrate (Floragard Vertriebs-GmbH, Oldenburg, Germany). Seedlings were grown in walk-in phytotrons (area: 9.5 m2), each containing four plexiglas subchambers (volume: 0.9 m3 each) (Vanzo et al., 2015), at the Research Unit Environmental Simulation (EUS), Helmholtz Zentrum München (Neuherberg, Germany) at ambient CO2 from 6th June to 27th July 2016. The initial conditions were 30°C (12/12 h, day/night), 50% relative humidity, and then changed to experimental conditions (12/12 h day/night regime: rh 5%/30%, temperature 40/20°C, soil temperature 15°C) within 3 days. After 2 weeks of acclimation, salt treatment started with successive increasing concentrations every second day from 100 mM to 200, 400 and 600 mM NaCl. RNA isolation for RNAseq was done as described in Paoletti et al. 2021. At the Biomedicum Functional Genomics Unit (FuGU, Helsinki, Finland), the shoot total RNA was ribo-depleted using ribo-zero rRNA removal (Plant leaf) (Illumina) and the root total RNA was ribo-depleted using ribo-zero rRNA removal (Plant Seed/Root) (Illumina). The total RNA input amounts used for ribo-depletion were 200 ng for shoot and 1 µg for root. The NEBnext ultra directional (polyA purification) Kit was used for library construction.
characteristics.characteristic: description
characteristics.text: LP.04 four leaves visible stage
characteristics.characteristic: developmental stage
characteristics.text: control
characteristics.characteristic: environmental stress
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Phoenix dactylifera
characteristics.characteristic: organism
characteristics.text: Phoenix dactylifera
characteristics.characteristic: organism
characteristics.text: shoot
characteristics.characteristic: organism part
characteristics.text: salt sensitive
characteristics.characteristic: phenotype
characteristics.text: Phoenix dactylifera
characteristics.characteristic: scientific_name
characteristics.text: Shoot_N_salt_ctrl_rep2
characteristics.characteristic: title