characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-09-14T20:17:34Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-09-14T20:17:34Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10535032
characteristics.characteristic: External Id
characteristics.text: Max Planck Institute for Infection Biology, Cellular Microbiology
characteristics.characteristic: INSDC center name
characteristics.text: 2022-09-14T20:17:34Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-09-14T20:17:34Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8187110
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11095:2 attP2 control rep2
characteristics.characteristic: Submitter Id
characteristics.text: 96
characteristics.unit: hour
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: P2AIII and attP2 (control) were crossed to uas-rpr (X). Crosses were put in collection cages containing apple juice agar plates at kept at 25oC in a 12h light/dark cycling incubator. 1st instar larvae were picked from apple agar plates and placed into vials containing standard fly at 100 larvae/15mL vial. Larvae were reared at 25oC in a 12h light/dark cycling incubator until further experimentation. 10 male larvae were collected at 3rd instar wandering stage across independent replicates per sample and snap-frozen in liquid N2. 10 male larvae were collected across independent replicates per sample and snap-frozen in liquid N2. Larvae were transferred to Lysing Matrix E homogenization tubes with 1ml of TRIzol and ruptured on high settings inf a FastPrep tissue homogenizer. The supernatant was transferred to a fresh tube and spun down for 2 minutes at max speed. 800µl of TRIzol sample was transferred to prespun phaselock tube and 200µl Chloroform added. Phases were separated by spinning at 12000 rcf, 15 min, 4°C. Upper aqueous phase was transferred to a fresh tube and mixed with 500 µl isopropanol. The resulting mix was spun at 20000 rcf, 15 min, 4°C. All supernatant was then drained and the pellet resuspended in 30 µl DNAse solution (Ambicon, final conc. 0.2U/µl) and incubate for 1h at 37°C. RNA was purified using the RNeasy Plus kit by addid 270µl RTL buffer and then isolating according the the manufacturers instructions. Libraries were generated by the Max Planck Genome Centre (Cologne). Libraries were stranded, from polyA enriched RNA.
characteristics.characteristic: description
characteristics.text: third instar larva stage
characteristics.characteristic: developmental stage
characteristics.text: attP2 control
characteristics.characteristic: genotype
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: wild type
characteristics.characteristic: phenotype
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: yw
characteristics.characteristic: strain
characteristics.text: 2 attP2 control rep2
characteristics.characteristic: title