characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-08-29T00:14:14Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-08-29T00:14:14Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110677157
characteristics.characteristic: External Id
characteristics.text: Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2022-08-29T00:14:14Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-08-29T00:14:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12774724
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12115:Control_1_lncRNA
characteristics.characteristic: Submitter Id
characteristics.text: 1
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: Muscovy
characteristics.characteristic: breed
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: mallard
characteristics.characteristic: common name
characteristics.text: Protocols: Twenty seven 1-day-old healthy Muscovy ducklings were collected and randomly separated into three groups. Nine ducklings were administrated with 500 μL JDm10 strain at a titer of 108.2 median tissue culture infective dose (TCID50) per mL via intramuscular injection; nine ducklings were administrated with 500 μL HN10 strain at a titer of 106.4 median TCID50 per mL; the rest of ducklings were treated with sterile DMEM as a blank control. The bursa of Fabricius specimens were harvested at 72 hours post infection (hpi) and frozen in liquid nitrogen for the consequent experiments. Total RNA of bursa of Fabricius tissues was extracted using RNAiso Plus (Takara Bio Inc., Dalian, China). The RNA amount and purity of each sample was quantified using NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA). The RNA integrity was assessed by Bioanalyzer 2100 (Agilent, CA, USA) with RIN number >7.0, and confirmed by electrophoresis with denaturing agarose gel. RNA was divided into two parts for at least 2 μg in each. Epicentre Ribo-Zero Gold Kit (Illumina, San Diego, USA) was used to deplete rRNA, and NEB Next Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, USA) was used to establish the library of one part for long chain RNA sequencing. TruSeq Small RNA Sample Prep Kit (Illumina) was used to establish the library of the other part for small RNA sequencing.
characteristics.characteristic: description
characteristics.text: neonatal
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Anas platyrhynchos
characteristics.characteristic: organism
characteristics.text: Anas platyrhynchos
characteristics.characteristic: organism
characteristics.text: bursa of Fabricius
characteristics.characteristic: organism part
characteristics.text: Anas platyrhynchos
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: Control_1_lncRNA
characteristics.characteristic: title