characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-09-06T00:18:32Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-09-06T00:18:32Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110169628
characteristics.characteristic: External Id
characteristics.text: Genome Engineering and Maintenance Network (CenGEM), College of Health, Medicine and Life Sciences, Division of Biosciences, Brunel University London
characteristics.characteristic: INSDC center name
characteristics.text: 2022-09-06T00:18:32Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-09-06T00:18:32Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12268442
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11851:K562-t(7;12)_rep1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: K562
characteristics.characteristic: cell line
characteristics.text: lymphoblast
characteristics.characteristic: cell type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: 1x10^6 cultured cells were pelleted by centrifugation at 400 g for 5 min. Pelleted cells were used for extraction. Total RNA was extracted using Monarch Total RNA Miniprep Kit according to manufacturer's instructions (New England Biolabs, Hitchin, UK) Library construction was performed by Macrogen Europe BV (Amsterdam, Netherlands). A minimum of 1 μg (20 ng/μl) of high-quality total RNA (extracted using Monarch Total RNA Miniprep Kit, NEB) was supplied for sequencing. Macrogen Europe BV constructed libraries using Illumina Truseq Stranded Total RNA library preparation with Ribozero rRNA depletion.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: chronic myelogenous leukemia
characteristics.characteristic: disease
characteristics.text: CRSPR/Cas9 generated t(7;12)(q36;p13)
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: bone marrow cell
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: K562-t(7;12)_rep1
characteristics.characteristic: title