characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-09-23T00:30:44Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-09-23T00:30:44Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110675913
characteristics.characteristic: External Id
characteristics.text: Wallenberg Centre for Molecular Medicine (WCMM), Linkoping University, Linkoping, Sweden Department of Biomedical and Clinical Sciences (BKV), Linkoping University, Linkoping, Sweden
characteristics.characteristic: INSDC center name
characteristics.text: 2022-09-23T00:30:44Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-09-23T00:30:44Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12773481
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12062:FOXQ1_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HCT116
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: HCT116 cells were grown on a 6 well-plate, treated with 10nM LGK974 and transfected with empty vector or FOXQ1. Flag-FOXQ1 and empty vector control (pCS2+N-Flag) were transfected using the jetOPTIMUS transfection reagents (Polyplus Transfection, Illkirch, France) according to the suppliers recommendations. HCT116 cells were plated in a 6-well plate and 1ug/well of each of the plasmids was used for transfection. After transfection, cells were treated overnight with 100 ng/mL recombinant human WNT3A protein. Each condition was performed in biological triplicates. Total RNA was isolated after 24 hours from transfection using a Qiagen RNeasy mini kit (Hilden, Germany). Library preparation was performed using the NEBNext RNA Library Prep Kit (NEB, UK).RNA and cDNA quality were assessed using the 2100 Bioanalyzer instrument (Agilent) and the Fragment analyzer System (Agilent) respectively.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: colorectal carcinoma
characteristics.characteristic: disease
characteristics.text: pCS2+N-Flag-FOXQ1
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: mixed large intestine and colon
characteristics.characteristic: organism part
characteristics.text: FOXQ1 overexpression
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: FOXQ1_1
characteristics.characteristic: title