characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-08-29T12:15:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-08-29T12:15:47Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110190581
characteristics.characteristic: External Id
characteristics.text: Universita degli Studi di Firenze, Facolta di Agraria
characteristics.characteristic: INSDC center name
characteristics.text: 2022-08-29T12:15:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-08-29T12:15:47Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12288259
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11885:Ed1
characteristics.characteristic: Submitter Id
characteristics.text: 10
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: About 100mg for each each sample were harvested, snap frozen in liquid nitrogen and stored at -80 °C till use for RNA-seq analyses. M. polymorpha plants were grown in a growth chamber in 1/10 modified Hoagland (Tennstedt et al. 2009, Fischer et al. 2014) solid medium under long-day photoperiod (16h light/8h dark) at 21 °C with a light intensity of 60 µmol m-2 s-1. 100 mg of frozen plant material was used for RNA extraction with the Spectrum Plant Total RNA Kit (Sigma-Aldrich®) according to the manufacturer's instructions. Amplification-grade DNaseI (Sigma-Aldrich®) treatment was carried out. 1.0 µg total RNA was used for library construction. Paired-end libraries were prepared with NEBNext® Ultra RNA Library Prep Kit (NEW ENGLAND BioLab) by integrating Illumina unique dual index adapters (Illumina).
characteristics.characteristic: description
characteristics.text: plantlet
characteristics.characteristic: developmental stage
characteristics.text: Cam2
characteristics.characteristic: ecotype
characteristics.text: Mppcs-2-16ge knockout mutant
characteristics.characteristic: genotype
characteristics.text: Marchantia polymorpha subsp. polymorpha
characteristics.characteristic: organism
characteristics.text: Marchantia polymorpha subsp. polymorpha
characteristics.characteristic: organism
characteristics.text: gametophyte
characteristics.characteristic: organism part
characteristics.text: Marchantia polymorpha subsp. polymorpha
characteristics.characteristic: scientific_name
characteristics.text: Ed1
characteristics.characteristic: title