characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-08-26T00:15:28Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-08-26T00:15:28Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110381734
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF CAMBRIDGE
characteristics.characteristic: INSDC center name
characteristics.text: 2022-08-26T00:15:28Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-08-26T00:15:28Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12479929
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11836_2:SIGAF9
characteristics.characteristic: Submitter Id
characteristics.text: 7
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: rabbit
characteristics.characteristic: common name
characteristics.text: Protocols: New Zealand White Rabbits (Oryctolagus cuniculus) from Envigo (RMS) UK Ltd, were mated at Labcorp Early Development Laboratories Limited (Eye, Suffolk) (formerly known as Covance Laboratories Limited) by natural mating. After mating each female was injected intravenously with 25 i.u. luteinizing hormone. Gestational day (GD)0 was defined as the day of mating. On GD7, 8 or 9 the pregnant rabbits were sacrificed, and the uteri were harvested and shipped fresh in Phosphate Buffered Saline (PBS) on ice to the Jeffrey Cheah Biomedical Centre. Embryos were dissected from the uteri at GD7, 8, and 9 using a Leica brightfield microscope and fine point tweezers using 10% heat-inactivated Fetal Bovine Serum (FBS) in PBS. Embryos were selected based on morphology matching developmental day of dissection. Selected embryos were rinsed in PBS, centrifuged for 100 x g for 3 minutes before being individually dissociated with TrypLE™ Express (Gibco™) by incubating for 6-10 minutes at 37°C with occasional agitation of the tube by flicking the base of the tube in order to ensure even dissociation of the embryo. Dissociation was quenched with 5mL 10% heat-inactivated FBS in PBS and filtered using a 30µm Sysmex CellTrics® filter. Cells were centrifuged for 300 x g for 3 minutes and resuspended in 0.04% BSA in PBS. Cells were filtered through a 40μm Flowmi tip strainer (VWR) to minimize volume loss during filtration and then counted using a haemocytometer. Steps were performed according to manufacturers' instructions (10X Genomics, v.3.1 chemistry). scRNA-seq libraries were generated by the CRUKCI genomics core facility using the 10X Genomics Chromium system (v.3.1 Chemistry)
characteristics.characteristic: description
characteristics.text: embryo stage
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: individual 6
characteristics.characteristic: individual
characteristics.text: Oryctolagus cuniculus
characteristics.characteristic: organism
characteristics.text: Oryctolagus cuniculus
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Oryctolagus cuniculus
characteristics.characteristic: scientific_name
characteristics.text: not available
characteristics.characteristic: sex
characteristics.text: New Zealand white rabbit
characteristics.characteristic: strain
characteristics.text: SIGAF9
characteristics.characteristic: title