characteristics.text: ERC000020
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-09-01T00:18:14Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-09-01T00:18:14Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110398716
characteristics.characteristic: External Id
characteristics.text: DUNDEE
characteristics.characteristic: INSDC center name
characteristics.text: 2022-09-01T00:18:14Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-09-01T00:18:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12496739
characteristics.characteristic: SRA accession
characteristics.text: Hv908
characteristics.characteristic: Submitter Id
characteristics.text: rural area [ENVO:01000772]
characteristics.characteristic: broad-scale environmental context
characteristics.text: 2015-02-16
characteristics.characteristic: collection date
characteristics.text: Barley Rhizosphere DNA sample of B1K-12 (wild) barley exposed to sufficient nitrogen supplies
characteristics.characteristic: description
characteristics.text: farm soil [ENVO:00005749]
characteristics.characteristic: environmental medium
characteristics.text: United Kingdom
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 56.45139
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: -3.074722
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: Barley
characteristics.characteristic: host common name
characteristics.text: 4513
characteristics.characteristic: host taxid
characteristics.text: research station [ENVO:00003919]
characteristics.characteristic: local environmental context
characteristics.text: Nutrient solutions were supplied by watering weekly at a rate of 25ml per Kg of soil. 100% N solution: 25mM 2(Nh4)SO4; 40mM Ca(NO3)2; 10mM KNO3; 3mM MgSO4; 100µM FeEDTA+; 1mM KH2PO4; 14 treatments were applied with a total of 312.5 mg (NO3-) and 81.4 mg (NH4+)
characteristics.characteristic: mineral nutrient regimen
characteristics.text: Hordeum vulgare
characteristics.characteristic: organism
characteristics.text: 515F: GTGCCAGCMGCCGCGGTAA; 806R: GGACTACHVGGGTWTCTAAT
characteristics.characteristic: pcr primers
characteristics.text: Barley NT
characteristics.characteristic: project name
characteristics.text: Hordeum vulgare
characteristics.characteristic: scientific_name
characteristics.text: MiSeq [OBI:0002003]
characteristics.characteristic: sequencing method
characteristics.text: Hv908
characteristics.characteristic: source material identifiers
characteristics.text: B1K-12
characteristics.characteristic: subspecific genetic lineage
characteristics.text: 16S rRNA
characteristics.characteristic: target gene
characteristics.text: V4
characteristics.characteristic: target subfragment
characteristics.text: Barley Rhizosphere sample Hv908
characteristics.characteristic: title