characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-09-20T08:17:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-09-20T08:17:36Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10169134
characteristics.characteristic: External Id
characteristics.text: Immuno-genomics & Systems Biology laboratory, Institute of Life Sciences (ILS), Bhubaneswar, Odisha, India-751023. Manipal Academy of Higher Education, Manipal,
characteristics.characteristic: INSDC center name
characteristics.text: 2022-09-20T08:17:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-09-20T08:17:36Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7825606
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10989:Sample 6
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: MutuDC1940
characteristics.characteristic: cell line
characteristics.text: dendritic cell
characteristics.characteristic: cell type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: DC Cell lines (CD8+ cDC1 control and NCoR1 KD) were grown in IMDM medium supplemented with 10% FBS and antibiotic solution, 5 x 10-5 M b-mercaptoethanol, sodium bicarbonate and HEPES. The cells were maintained at 37°C in a humidified incubator with 5% CO2. These DCs were dissociated with a short incubation of 2-3 min in a nonenzymatic, 5 mM EDTA in 20 mM HEPES buffer. Cells were plated in 6-well plates at a density of 5 x 105 cells/ml overnight. The cells were then challenged with different activation media containing IFNg, TLR3 agonist pIC, and CpG+pIC+IFNg for 2hr or 6hr. For performing RT-qPCR analysis the cells were washed in the plate once with PBS followed by addition of RNA-later (RLT) buffer (Qiagen) for lysis of cells. The plates were then stored at -80oC until further RNA isolation and processing of samples. mRNA was isolated from 2ug of total RNA using magnetic beads mRNA isolation kit (PolyA mRNA isolation Module, NEB) RNA-seq library preparation was performed using mRNA library preparation kit (NEB)
characteristics.characteristic: description
characteristics.text: CD8alpha-positive
characteristics.characteristic: immunophenotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: spleen
characteristics.characteristic: organism part
characteristics.text: wild type
characteristics.characteristic: phenotype
characteristics.text: empty vector
characteristics.characteristic: rna interference
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Sample 6
characteristics.characteristic: title