characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-27T14:00:27Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-27T14:00:27Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111452600
characteristics: External Id
characteristics: Department of Gene Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health,SciLifeLab, KTH Royal Institute of Technology, Stockholm, Sweden
characteristics: INSDC center name
characteristics: 2022-10-27T14:00:27Z
characteristics: INSDC first public
characteristics: 2022-10-27T14:00:27Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13547146
characteristics: SRA accession
characteristics: E-MTAB-12261:Het
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: Protocols: Cells were collected in exponential phase of the growth concentrated by centrifugation at 8000g for 5 min. Libraries were prepared with Magnatrix 8000+ (Nordiag), an eight-channel robotic workstation capable of running custom made scripts for in-tip magnetic bead separations41. Released mRNA-cDNA hybrids were transferred to the robotic workstation where they underwent second strand synthesis, end repair and in vitro transcription. Following transcription, sequencing adaptors are ligated to the RNA, and another round of cDNA synthesis is performed. Each step in the process is followed by a reaction clean-up step.
characteristics: description
characteristics: Heterocapsa sp.
characteristics: organism
characteristics: Heterocapsa sp.
characteristics: organism
characteristics: Heterocapsa sp.
characteristics: scientific_name
characteristics: 164
characteristics: spots with single cell detected
characteristics: CCAP 1125/4
characteristics: strain
characteristics: Het
characteristics: title