characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-14T00:35:00Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-14T00:35:00Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111431750
characteristics: External Id
characteristics: Department of Dermatology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland
characteristics: INSDC center name
characteristics: 2022-10-14T00:35:00Z
characteristics: INSDC first public
characteristics: 2022-10-14T00:35:00Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13525425
characteristics: SRA accession
characteristics: E-MTAB-12237:51_0
characteristics: Submitter Id
characteristics: 59
characteristics: year
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: human
characteristics: common name
characteristics: Protocols: Donor-matched skin biopsies were taken from non-lesional (NL) and Lesional human skin of positive patch-test reactions to nickel at 24h, 48h, and 120h post allergen application. Biopsies were embedded in OCT cryo-embedding matrix, frozen in liquid nitrogen, and stored at -80°C until use RNA-seq reads were mapped to the human reference genome (GRCh38, build 81) using HISAT2 v. 2.0.4 (Kim et al., 2015). Total RNA was isolated with Blood & Tissue Kit (Qiagen). Illumina TruSeq Stranded mRNA protocol
characteristics: description
characteristics: allergic contact dermatitis
characteristics: disease
characteristics: 51
characteristics: individual
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: organism
characteristics: skin
characteristics: organism part
characteristics: normal tissue
characteristics: sampling site
characteristics: Homo sapiens
characteristics: scientific_name
characteristics: female
characteristics: sex
characteristics: 51_0
characteristics: title