characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-27T14:00:03Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-27T14:00:03Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111452526
characteristics: External Id
characteristics: Department of Gene Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health,SciLifeLab, KTH Royal Institute of Technology, Stockholm, Sweden
characteristics: INSDC center name
characteristics: 2022-10-27T14:00:03Z
characteristics: INSDC first public
characteristics: 2022-10-27T14:00:03Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13545924
characteristics: SRA accession
characteristics: E-MTAB-12262:Heterocapsa RNA
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: Protocols: Sample for bulk RNA was collected by filtering 10 ml of culture in exponential phase (density of cells ≈ 10 mil) on 5 µm polycarbonate filters (Whatman).Filters were placed in cryoprotectant tubes with beads (Zirconium oxide beads, Precellys), amended with 600 µl of RLT buffer (Qiagen) which contained 1% of 2-Mercaptoethanol, shock frozen in liquid nitrogen and stored at -80 °C until further processing. Prior to RNA extraction, sample was taken out of -80, thawed on ice and subjected to bead beating (1x 6000 rmp, Precellys Evolution homogenizer)for 2 min. RNA from samples was further extracted with the RNeasy Qiagen kit (QIAGEN, Inc., Valencia, CA) following the kit instructions. RNA was re-eluted in 30 µl of nuclease free water. Input: RNA Type: Illumina TruSeq Stranded mRNA Option: Poly-A selection Category: default
characteristics: description
characteristics: Heterocapsa sp.
characteristics: organism
characteristics: Heterocapsa sp.
characteristics: organism
characteristics: Heterocapsa sp.
characteristics: scientific_name
characteristics: dinoflagellate Heterocapsa sp. CCAP 1125/4
characteristics: strain
characteristics: Heterocapsa RNA
characteristics: title