characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-25T00:15:12Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-25T00:15:12Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111280599
characteristics: External Id
characteristics: Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology
characteristics: INSDC center name
characteristics: 2022-10-25T00:15:12Z
characteristics: INSDC first public
characteristics: 2022-10-25T00:15:12Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13378196
characteristics: SRA accession
characteristics: E-MTAB-12134:CoV2_RNA_6h_R1
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: Calu3
characteristics: cell_line
characteristics: epithelial cell
characteristics: cell_type
characteristics: human
characteristics: common name
characteristics: Protocols: Calu-3 cells were grown in complete DMEM-F12 media in a humidified incubator at 37 °C in the presence of 5% CO2. The medium was replaced with DMEM-F12 containing 0.2% BSA and Pen-Strep prior to infection experiments. The cells were mock- or virus-infected at an MOI of 0.1 for 0, 3, 6, 12, 24, and 48 hours. At each time point, the samples were washed twice with 1x TBS buffer and harvested in QIAzol lysis reagent (Qiagen, Germany)followed by DNAse digestion (Qiagen, Germany) treatment on the RNeasy Mini columns, according to the manufacturer’s protocol. The amount of total RNA was quantified using the Qubit 2.0 Fluorometric Quantitation system (Thermo Fisher Scientific, Waltham, MA, USA), and the RNA integrity number (RIN) was determined using the Experion Automated Electrophoresis System (Bio-Rad, Hercules, CA, USA). RNA-seq libraries were prepared with the TruSeq Stranded mRNA LT sample preparation kit (Illumina, San Diego, CA, USA) using Sciclone and Zephyr liquid handling workstations (PerkinElmer, Waltham, MA, USA) for pre- and post-PCR steps, respectively. Library concentrations were quantified with the Qubit 2.0 Fluorometric Quantitation system (Life Technologies, Carlsbad, CA, USA). The size distribution was assessed using the Experion Automated Electrophoresis System (Bio-Rad, Hercules, CA, USA). For sequencing, samples were diluted and pooled into NGS libraries in equimolar amounts.
characteristics: description
characteristics: lung adenocarcinoma
characteristics: disease
characteristics: wild type genotype
characteristics: genotype
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: scientific_name
characteristics: CoV2_RNA_6h_R1
characteristics: title