characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-12-08T14:37:16Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-12-08T14:37:16Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110643084
characteristics: External Id
characteristics: Nantes Universite, CHU Nantes, CNRS, INSERM, l’institut du thorax, F-44000 Nantes, France
characteristics: INSDC center name
characteristics: 2022-12-08T14:37:16Z
characteristics: INSDC first public
characteristics: 2022-12-08T14:37:16Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12740849
characteristics: SRA accession
characteristics: E-MTAB-11817:C2-DC2093
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: iPS.C2a
characteristics: cell_line
characteristics: cardiocyte
characteristics: cell_type
characteristics: human
characteristics: common name
characteristics: Protocols: After 30 days of hiPSC cardiac differentiation, for each sample, cells were harvested from three distinct beating wells, dissociated using the Multi Tissue Dissociation Kit 3 (Miltenyi Biotec), and pooled. Cell suspensions were filtered on a 40 µm Flowmi® Cell Strainer, counted and cell viability was assessed. For each sample, single-cell droplet libraries were generated from 16000 cells with the Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3 (10X Genomics). For each sample, single-cell droplet libraries were generated from 16000 cells with the Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3 (10X Genomics).
characteristics: description
characteristics: infant
characteristics: developmental stage
characteristics: normal
characteristics: disease
characteristics: wild type genotype
characteristics: genotype
characteristics: cardiac differentiation condition
characteristics: growth condition
characteristics: C2-DC2093
characteristics: individual
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: organism
characteristics: skin of prepuce of penis
characteristics: organism part
characteristics: induced pluripotent stem cell
characteristics: progenitor cell type
characteristics: 30
characteristics: day
characteristics: sampling time point
characteristics: Homo sapiens
characteristics: scientific_name
characteristics: male
characteristics: sex
characteristics: C2-DC2093
characteristics: title