characteristics: ERC000035
characteristics: ENA-CHECKLIST
characteristics: 2022-11-14T15:53:09Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-11-14T15:53:09Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111385077
characteristics: External Id
characteristics: ENS Lyon
characteristics: INSDC center name
characteristics: 2022-11-14T15:53:09Z
characteristics: INSDC first public
characteristics: 2022-11-14T15:53:09Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13482055
characteristics: SRA accession
characteristics: NP22
characteristics: Submitter Id
characteristics: Protoplasts isolated from maize leaf 2 weeks after sowing
characteristics: age
characteristics: Leaf protoplast
characteristics: cell_type
characteristics: maize
characteristics: common name
characteristics: Maize inbred line A188
characteristics: cultivar
characteristics: Targeted mutagenesis of genes Zm00001e011125 and Zm00001e021494 (GeneID v5). Target sequences: ACTCCTCAACGTGGGCGTGTTCGG and GACGAAGACGCTGACGGAGAAGG. Forward primer: CAGCTGTTCACGGCCAAGAT. Reverse primer: GCGCTTCTGAAATTGAGGACTG. Expected size: 199bp.
characteristics: description
characteristics: 48h after transfection
characteristics: dev_stage
characteristics: Transfection with CRISPR/Cas9 constructs
characteristics: genetic modification
characteristics: Maize inbred line A188
characteristics: genotype
characteristics: Protoplasts in W5 medium, at 26�C, in the dark
characteristics: growth condition
characteristics: NP22
characteristics: individual
characteristics: Zea mays subsp. mays
characteristics: organism
characteristics: Leaf
characteristics: organism part
characteristics: Diploid
characteristics: ploidy
characteristics: Fierlej et al.
characteristics: protocol
characteristics: 48h after transfection
characteristics: sampling time point
characteristics: Zea mays subsp. mays
characteristics: scientific_name
characteristics: Hermaphrodite
characteristics: sex
characteristics: Target genes: Sweet14a and Sweet14b
characteristics: title