characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-12-01T00:22:49Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-12-01T00:22:49Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA103981497
characteristics: External Id
characteristics: OREGON STATE UNIVERS
characteristics: INSDC center name
characteristics: 2022-12-01T00:22:49Z
characteristics: INSDC first public
characteristics: 2022-12-01T00:22:49Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS1670665
characteristics: SRA accession
characteristics: E-MTAB-5689:Seedling-shoot-8-days-dark-replicate-1
characteristics: Submitter Id
characteristics: 8
characteristics: day
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: Japanese rice
characteristics: common name
characteristics: Nipponbare
characteristics: cultivar
characteristics: Protocols: The chamber was set to cycle between 20C for 12 hours and 18C for the next 12 hours, and the soil was kept moist by gently spraying with water every 72 hours. Sampling was done at about 7.30 pm under green lights. Continuous dark: Samples harvested after growing the seedlings in continuous dark for 8 days (8DD) after germination. Total RNA from frozen seedling shoot samples was extracted as per manufacturer's protocol for RNA Plant reagent (Invitrogen Inc., USA), RNeasy kits (Qiagen Inc., USA), and RNase-free DNase (Life Technologies Inc., USA). True-Seq kit (Illumina Inc.) was used to prepare RNA-seq libraries, according to the manufacturer’s protocol.
characteristics: description
characteristics: LP.02 two leaves visible stage
characteristics: developmental stage
characteristics: continuous dark (no light) regimen
characteristics: growth condition
characteristics: Oryza sativa Japonica Group
characteristics: organism
characteristics: Oryza sativa Japonica Group
characteristics: organism
characteristics: shoot
characteristics: organism part
characteristics: Oryza sativa Japonica Group
characteristics: scientific_name
characteristics: Seedling-shoot-8-days-dark-replicate-1
characteristics: title