characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-12-14T00:28:02Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-12-14T00:28:02Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111404589
characteristics: External Id
characteristics: Biomics Platform, Institut Pasteur
characteristics: INSDC center name
characteristics: 2022-12-14T00:28:02Z
characteristics: INSDC first public
characteristics: 2022-12-14T00:28:02Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13498465
characteristics: SRA accession
characteristics: E-MTAB-12210:xen36
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: Protocols: Bacteria were grown in brain heart infusion (BHI) broth (BD) with shaking at 200 rpm at 37째C overnight. Bacterial suspensions were diluted in BHI broth and bacteria grown to OD 600nm=2 with shaking at 200 rpm at 37째C. Genomic DNA was isolated from bacteria using the PureLink DNeasy kit (Invitrogen) with the addition of 0.5 mg/mL (100mg/mL) lysozyme and 0.05 ng/mL (100-500ug/mL?) lysostaphin to the lysis buffer. Libraries were prepared using PacBio Barcoded Adapters for Multiplex SMRT Sequencing protocol (3.0 chemistry)
characteristics: description
characteristics: Staphylococcus aureus subsp. aureus MRSA252
characteristics: organism
characteristics: Staphylococcus aureus subsp. aureus MRSA252
characteristics: organism
characteristics: Staphylococcus aureus subsp. aureus MRSA252
characteristics: scientific_name
characteristics: Xen36
characteristics: strain
characteristics: xen36
characteristics: title