characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-27T00:17:18Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-27T00:17:18Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111479023
characteristics: External Id
characteristics: Center of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, University of Zurich
characteristics: INSDC center name
characteristics: 2022-10-27T00:17:18Z
characteristics: INSDC first public
characteristics: 2022-10-27T00:17:18Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13573058
characteristics: SRA accession
characteristics: E-MTAB-12271:Sample 1
characteristics: Submitter Id
characteristics: 16
characteristics: week
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: house mouse
characteristics: common name
characteristics: Protocols: Cells were harvested by trypsinization, washed with PBS and mixed in RNA lysis buffer (Zymo Research). Cardiac tissue was lysed in 600 µL of RNA lysis buffer (Zymo) and homogenized using TissueLyserII (Qiagen). Homogenate was spun down for 2 min at 15000 g and the supernatant was processed according to the manufacturer’s instructions. Zymo Quick-RNA MicroPrep isolation kit was used to extract total RNA. 100% ethanol was added to the lysates and samples were than processed on the columns. DNA contamination was removed by DNase I digestion. RNA was washed and eluted in 20 µl of water. RNA concentration and purity were assessed with Nanodrop 1000 (Thermo Fisher Scientific) RNA-seq library preparation workflow Figure 2.2.1 Strand-specific RNA sequencing with polyA selection.
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: impulse control disorder
characteristics: disease
characteristics: Rag2
characteristics: genotype
characteristics: Mus musculus
characteristics: organism
characteristics: Mus musculus
characteristics: organism
characteristics: heart
characteristics: organism part
characteristics: Mus musculus
characteristics: scientific_name
characteristics: BTBR mouse
characteristics: strain
characteristics: Sample 1
characteristics: title