characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-09-29T00:17:16Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-09-29T00:17:16Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110177348
characteristics: External Id
characteristics: INSERM GUSTAVE ROUSSY Institute
characteristics: INSDC center name
characteristics: 2022-09-29T00:17:16Z
characteristics: INSDC first public
characteristics: 2022-09-29T00:17:16Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12276156
characteristics: SRA accession
characteristics: E-MTAB-11036:S21_S4
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: Non applicable
characteristics: cell line
characteristics: hematopoietic cell from IPSC
characteristics: cell type
characteristics: human
characteristics: common name
characteristics: Protocols: Hematopoietic cells in the supernatant of cultures at day 13 of differentiation were purified for cell viability using Sytox blue. Sample preparation was done at room temperature. Single-cell suspensions were loaded onto a Chromium Single Cell Chip (10x Genomics) according to the manufacturer’s instructions for co-encapsulation with barcoded Gel Beads at a target capture rate of ~10,000 individual cells per sample. Captured mRNAs were barcoded during cDNA synthesis using the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10X Genomics) according to the manufacturer’s instructions. All samples were processed simultaneously with the Chromium Controller (10X Genomics) and the resulting libraries were prepared in parallel in a single batch.
characteristics: description
characteristics: embryo stage
characteristics: developmental stage
characteristics: Down syndrome
characteristics: disease
characteristics: disomic with GATA1s mutation,MPL mutation and SMC3 mutation
characteristics: genotype
characteristics: Myeloid, Erythroid, Megakaryocyte
characteristics: inferred cell type
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: organism
characteristics: Non applicable
characteristics: organism part
characteristics: Homo sapiens
characteristics: scientific_name
characteristics: S21_S4
characteristics: title