characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-09-30T04:31:42Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-09-30T04:31:42Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111412451
characteristics: External Id
characteristics: PEKING UNIVERSITY
characteristics: INSDC center name
characteristics: 2022-09-30T04:31:42Z
characteristics: INSDC first public
characteristics: 2022-09-30T04:31:42Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13506116
characteristics: SRA accession
characteristics: E-MTAB-12215:Sample 3
characteristics: Submitter Id
characteristics: 8
characteristics: week
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: house mouse
characteristics: common name
characteristics: Protocols: Small intestines were isolated from mice and whole cell were subjected to RNA extraction. RNAs were extracted with TRIzol. Sequencing libraries were generated using VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina ® (NR604-01/02) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Then we added fragmentation buffer to break the mRNA into short fragments. First strand cDNA was synthesized using random hexamer primer and RNase H. Second strand cDNA synthesis was subsequently performed using buffer, dNTPs, DNA polymerase I and RNase H. And then, the double stranded cDNA was purified byAMPure P beads or QiaQuick PCR kit. The purified double stranded cDNA was repaired at the end, added a tail and connected to the sequencing connector, then the fragment size was selected, and finally the final cDNA library was obtained by PCR enrichment.
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: wild type genotype
characteristics: genotype
characteristics: germ-free
characteristics: growth condition
characteristics: Mus musculus
characteristics: organism
characteristics: Mus musculus
characteristics: organism
characteristics: small intestine
characteristics: organism part
characteristics: Mus musculus
characteristics: scientific_name
characteristics: female
characteristics: sex
characteristics: c57BL/6
characteristics: strain
characteristics: Sample 3
characteristics: title