characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-09-30T00:20:41Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-09-30T00:20:41Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110177341
characteristics: External Id
characteristics: INSERM Gustave Roussy Institute
characteristics: INSDC center name
characteristics: 2022-09-30T00:20:41Z
characteristics: INSDC first public
characteristics: 2022-09-30T00:20:41Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12276149
characteristics: SRA accession
characteristics: E-MTAB-11032:T104_GATA1
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: Non applicable
characteristics: cell line
characteristics: CD34-negative, CD41-positive, CD42-positive megakaryocyte cell
characteristics: cell type
characteristics: human
characteristics: common name
characteristics: Protocols: Cut&Tag against GATA1 was performed on CD41+CD42+ megakaryocytes obtained after 18 days of differentiation of the IPSC clones. 500,000 CD41+CD42+ MK sorted cells were used to analyze GATA1 and GATA1s chromatin occupancy using the CUT&Tag-IT Assay Kit (Active Motif) according to the manufacturer recommendations. Briefly, cells were bound to Concanavalin A-Coated Beads and incubated with primary anti-GATA1 antibody in buffer with Protease Inhibitor Cocktail and 5% digitonine overnight at 4°C under rotation. The Guinea Pig anti-rabbit secondary antibody was incubated in Dig-Wash buffer for 1 hour at RT under rotation. After 3 washes, the CUT&Tag-IT™ Assembled pA-Tn5 Transposomes (1:100) were added for 1 hour at RT under rotation and tagmentation was performed during 1 hour at 37°C. DNA was purified. Libraries were generated by PCR as for for all Tn5 based libraries. The final libraries were purified, pooled together in equal concentrations
characteristics: description
characteristics: GATA1s
characteristics: developmental stage
characteristics: Down syndrome
characteristics: disease
characteristics: Trisomic with GATA1s mutation
characteristics: genotype
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: organism
characteristics: Non applicable
characteristics: organism part
characteristics: Homo sapiens
characteristics: scientific_name
characteristics: T104_GATA1
characteristics: title