characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-01T16:26:18Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-01T16:26:18Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110329240
characteristics: External Id
characteristics: Department of Biochemistry, University of Zurich, CH-8057 Zurich, Switzerland
characteristics: INSDC center name
characteristics: 2022-10-01T16:26:18Z
characteristics: INSDC first public
characteristics: 2022-10-01T16:26:18Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12426325
characteristics: SRA accession
characteristics: E-MTAB-11952:UZH2_Rep1
characteristics: Submitter Id
characteristics: 20
characteristics: year
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: MOLM-13
characteristics: cell line
characteristics: malignant cell
characteristics: cell type
characteristics: human
characteristics: common name
characteristics: Protocols: Cells were cultured in RPMI 1640 medium (11875093, Thermo Fisher Scientific) containing 10 % FBS (16140071, Thermo Fisher Scientific) and 1 % penicillin-streptomycin (15140122, Thermo Fisher Scientific) in 5 % CO2 at 37 °C in a humidified incubator. Cells were seeded at a density of 200000 cells/mL in 5 mL of the complete medium and treated with 10 μM UZH2 or DMSO (5%) for 16 h. Before collection, cells were washed twice with PSB. Cell pellets were collected and total RNA was immediately isolated. Total RNA was isolated with RNeasy Kits (74104, QIAGEN). mRNA enrichment and purification was performed with Oligo dT selection. The RNA was fragmented and reverse transcription was done using random N6 primers (second-strand cDNA synthesis with dUTP instead of dTTP).This was followed end repair, add A and adaptor ligation. PCR was performed, followed by single strand separation and cyclization. The final step was the DNA nanoball synthesis.
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: acute myeloid leukemia
characteristics: disease
characteristics: wild type genotype
characteristics: genotype
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: organism
characteristics: blood
characteristics: organism part
characteristics: Homo sapiens
characteristics: scientific_name
characteristics: male
characteristics: sex
characteristics: UZH2_Rep1
characteristics: title