characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-11-01T00:15:32Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-11-01T00:15:32Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110219023
characteristics: External Id
characteristics: THE JACKSON LABORATORY
characteristics: INSDC center name
characteristics: 2022-11-01T00:15:32Z
characteristics: INSDC first public
characteristics: 2022-11-01T00:15:32Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12316651
characteristics: SRA accession
characteristics: E-MTAB-11902:Sample 1
characteristics: Submitter Id
characteristics: 10
characteristics: week
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: mesenchymal cell
characteristics: cell type
characteristics: house mouse
characteristics: common name
characteristics: Protocols: Naïve and tumor-bearing CD140a-EGFP mice were euthanized, and lung was harvested and digested. Lung single cell suspension was prepared and stained with primary-fluorophore conjugated antibodies. Lung CD140a+ mesenchymal cells (CD45-CD31-CD326-GFP+) were isolated by fluorescence-activated cell sorting. Cells capture, lysis and RNA extraction were performed using the 10x V3 Chromium technology. For each sample, around 12,000 cells were loaded in each lane of the 10x Chromium chip. Barcoding and library preparation were performed with the 10x Chromium system using version 3 chemistry, following the manufacturer’s protocol. cDNA was synthesized and amplified as per manufacturer’s protocol and amplified to construct an Illumina sequencing library. cDNA and libraries were checked for quality on an Agilent 4200 TapeStation, quantified by KAPA qPCR.
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: Pdgfratm11(EGFP)Sor/Pdgfratm11(EGFP)Sor
characteristics: genotype
characteristics: CD45-; CD31-; CD326-; GFP+
characteristics: immunophenotype
characteristics: 1
characteristics: individual
characteristics: Mus musculus
characteristics: organism
characteristics: Mus musculus
characteristics: organism
characteristics: lung
characteristics: organism part
characteristics: Mus musculus
characteristics: scientific_name
characteristics: female
characteristics: sex
characteristics: B6.129S4-Pdgfratm11(EGFP)Sor/J
characteristics: strain
characteristics: Sample 1
characteristics: title