characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-12-19T00:25:06Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-12-19T00:25:06Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110447766
characteristics: External Id
characteristics: Friedrich Schiller University Jena Institute of Biodiversity Aquatic Geomicrobiology
characteristics: INSDC center name
characteristics: 2022-12-19T00:25:06Z
characteristics: INSDC first public
characteristics: 2022-12-19T00:25:06Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12545796
characteristics: SRA accession
characteristics: E-MTAB-12015:Cocktail.1
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: Protocols: Biomass was harvested from mid-exponential cultures by means of centrifugation. Beijerinckiaceae bacterium RH AL1 was grown using MM2 medium to mid-exponential phase, using methanol (0.5%, v/v) as carbon and energy source. Cultures were supplemented with either different concentrations of lanthanum (50 nM, 1 µM) or different lanthanide species (La, Nd, lanthanide cocktail [La, Ce, Nd, Dy, Ho, Er, Yb]; 1 µM). Total RNA was extracted with TRI reagent (Applied Biosystems), mRNA was enriched using the RiboMinus bacteria 2.0 transcriptome isolation kit (Invitrogen). Sequencing libraries were prepared with the NEBNext Ultra II Directional RNA library prep kit for Illumina (New England Biolabs).
characteristics: description
characteristics: wild type genotype
characteristics: genotype
characteristics: grown to exponential phase with methanol as carbon and energy source
characteristics: growth condition
characteristics: Beijerinckiaceae bacterium RH AL1
characteristics: organism
characteristics: Beijerinckiaceae bacterium RH AL1
characteristics: organism
characteristics: whole organism
characteristics: organism part
characteristics: 1
characteristics: replicate
characteristics: Beijerinckiaceae bacterium RH AL1
characteristics: scientific_name
characteristics: Cocktail.1
characteristics: title