characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-12-01T00:19:33Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-12-01T00:19:33Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA10083777
characteristics: External Id
characteristics: VICTOR CHANG CARDIAC RESEARCH INSTITUTE
characteristics: INSDC center name
characteristics: 2022-12-01T00:19:33Z
characteristics: INSDC first public
characteristics: 2022-12-01T00:19:33Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS7740580
characteristics: SRA accession
characteristics: E-MTAB-10929:129T2 E16.5 replicate 1
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: house mouse
characteristics: common name
characteristics: Protocols: Embryos were dissected from the uterus in ice-cold PBS under a dissecting scope (Leica MZ8) using micro-tweezers. Once the heart was harvested from the embryo and the pericardial membrane was removed, the atrial and ventricular regions were separated by splitting the heart into 2 halves through the atrioventricular canal using micro-dissection tungsten needles. Then, the auricular appendages were cut off the atrial tissue and the remaining mesenchymal tissue from the atrioventricular canal was further trimmed. The remaining tissue, containing the atrial septum, was stored at -80ºC in RNAlater solution (Ambion) for further analysis. Total RNA was purified using miRNeasy Kit (Qiagen). RNA libraries were made from 300-400ng of total RNA using TruSeq (ribozero) stranded RNA Library Preparation Kit following the manufacturers protocol (Illumina).
characteristics: description
characteristics: embryonic day 16.5
characteristics: developmental stage
characteristics: normal
characteristics: disease
characteristics: wild type genotype
characteristics: genotype
characteristics: Mus musculus
characteristics: organism
characteristics: Mus musculus
characteristics: organism
characteristics: interventricular septum
characteristics: organism part
characteristics: Mus musculus
characteristics: scientific_name
characteristics: 129T2/SvEms
characteristics: strain
characteristics: 129T2 E16.5 replicate 1
characteristics: title