characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-09-29T00:16:29Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-09-29T00:16:29Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA10370030
characteristics: External Id
characteristics: Department of Biochemistry University of Cambridge
characteristics: INSDC center name
characteristics: 2022-09-29T00:16:29Z
characteristics: INSDC first public
characteristics: 2022-09-29T00:16:29Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS8024726
characteristics: SRA accession
characteristics: E-MTAB-11046:gh2_14
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: Protocols: 5X10^7 exponentially growing (5.5hrs post-inoculation) and early post-diauxic shift (13hrs post-inoculation for WT and hda1Δ, 14.5hrs post-inoculation for gcn5Δ and gcn5Δhda1Δ) cells were harvested be centrifugation at 16,100xg for 1 min at 4°C. Total RNA was isolated using an RNeasy Mini kit (Qiagen, 74104). After the QC procedures of total RNA, mRNA is enriched using oligo(dT) beads. Enriched mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is first synthesized by using random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.
characteristics: description
characteristics: early post-diauxic shift phase
characteristics: developmental stage
characteristics: gcn5 and hda1 deletion
characteristics: genotype
characteristics: glucose starved
characteristics: growth condition
characteristics: Saccharomyces cerevisiae S288C
characteristics: organism
characteristics: Saccharomyces cerevisiae S288C
characteristics: organism
characteristics: whole organism
characteristics: organism part
characteristics: Saccharomyces cerevisiae S288C
characteristics: scientific_name
characteristics: BY4742
characteristics: strain
characteristics: gh2_14
characteristics: title