characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-12-08T14:37:13Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-12-08T14:37:13Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110654449
characteristics: External Id
characteristics: Nantes Universite, CHU Nantes, CNRS, INSERM, l’institut du thorax, F-44000 Nantes, France
characteristics: INSDC center name
characteristics: 2022-12-08T14:37:13Z
characteristics: INSDC first public
characteristics: 2022-12-08T14:37:13Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12752031
characteristics: SRA accession
characteristics: E-MTAB-11822:04_70_DC2650_WT8288-C04_D4
characteristics: Submitter Id
characteristics: 55
characteristics: year
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: ITXi006-A
characteristics: cell_line
characteristics: induced pluripotent stem cell
characteristics: cell_type
characteristics: human
characteristics: common name
characteristics: Protocols: Directed cardiac differentiations of hiPSCs were performed using the established matrix sandwich method (Es-Salah-Lamoureux et al., 2016). Briefly, when hiPSCs reached 90% confluency, an overlay of Growth Factor Reduced Matrigel (0.033 mg/ml, BD Corning) was added. Differentiation was initiated 24h later by culturing the cells in RPMI1640 medium (Life Technologies) supplemented with B27 (without insulin, Life Technologies), 2 mM L-glutamine (Life Technologies), 1% NEAA (Life Technologies), 100 ng/mL Activin A (Miltenyi Biotec), 1X Pen/Strep (Life Technologies) and 10 ng/mL FGF2 for 24 hours. On the next day, the medium was replaced by RPMI1640 medium supplemented with B27 without insulin, 2 mM L-glutamine, 1% NEAA, 10 ng/mL BMP4 (Miltenyi Biotec), 1X Pen/Strep and 5 ng/mL FGF2 for 4 days. By day 5, cells were cultured in RPMI1640 medium supplemented with B27 complete (Life Technologies), 2 mM L-glutamine, 1X Pen/Strep and 1% NEAA and changed every two days until day 30. For each hiPSC line, samples were harvested daily from D-1 to D30 of the cardiac differentiation protocol from three independent cardiac differentiations. Total RNA were extracted using the NucleoSpin RNA kit (MACHEREY-NAGEL) and their quality assessed by NanoDropTM 1000 Spectrophotometer (Thermo Fisher Scientific). 3’RNA libraries were prepared by GenoBiRD core facility according to their published method (Charpentier et al., 2021)
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: normal
characteristics: disease
characteristics: wild type genotype
characteristics: genotype
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: organism
characteristics: blood
characteristics: organism part
characteristics: mononuclear cell
characteristics: progenitor cell type
characteristics: Homo sapiens
characteristics: scientific_name
characteristics: male
characteristics: sex
characteristics: 04_70_DC2650_WT8288-C04_D4
characteristics: title