characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-12-06T00:16:44Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-12-06T00:16:44Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110378178
characteristics: External Id
characteristics: Bielefeld University, Center for Biotechnology (CeBiTec), Universitatsstraße 27, 33615, Bielefeld, Germany Bielefeld University, Medical Faculty OWL Universitatsstraße 27, 33615, Bielefeld, Germany
characteristics: INSDC center name
characteristics: 2022-12-06T00:16:44Z
characteristics: INSDC first public
characteristics: 2022-12-06T00:16:44Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12476374
characteristics: SRA accession
characteristics: E-MTAB-11968:Streptococcus pneumoniae D39 wild type control 2
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: Protocols: The S. pneumoniae D39 wild type strain was cultivated in RPMI medium and treated with various thiol-reactive compounds at an OD600 of 0.4 for 30 min. Bacteria were harvested in ice-cold killing buffer (50 mM Tris pH 7.5, 5 mM MgCl2, 20 mM NaN3), centrifuged at 4,750 rpm for 10 min at 4°C. The pellets were immediately frozen in liquid nitrogen and stored at -80°C. RNA isolation was performed using the acidic phenol-chloroform extraction protocol as described previously (1). After DNase-I treatment (Zymo Research, Germany), the RNA quality was checked by Trinean Xpose (Gentbrugge, Belgium) and the Agilent RNA Nano 6,000 kit using an Agilent 2,100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). 1. Wetzstein M, Volker U, Dedio J, Lobau S, Zuber U, Schiesswohl M, Herget C, Hecker M, Schumann W. Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis. J Bacteriol 174: 3300-10, 1992. For RNA-seq transcriptomics, Ribo-Zero rRNA Removal Kit (Bacteria) from Illumina (San Diego, CA, USA) was used to remove the rRNA. TruSeq Stranded mRNA Library Prep Kit from Illumina (San Diego, CA, United States) was applied to prepare the cDNA libraries.
characteristics: description
characteristics: logarithmic phase culture
characteristics: growth condition
characteristics: Streptococcus pneumoniae D39
characteristics: organism
characteristics: Streptococcus pneumoniae D39
characteristics: organism
characteristics: Streptococcus pneumoniae D39
characteristics: scientific_name
characteristics: Streptococcus pneumoniae D39 wild type control 2
characteristics: title