characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-01T16:25:11Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-01T16:25:11Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA10371405
characteristics: External Id
characteristics: Institut fur Biomedizinische Alternsforschung, Leopold-Franzens-Universitat Innsbruck
characteristics: INSDC center name
characteristics: 2022-10-01T16:25:11Z
characteristics: INSDC first public
characteristics: 2022-10-01T16:25:11Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS8026098
characteristics: SRA accession
characteristics: E-MTAB-11045:daf-2(+)_egl-8(-)
characteristics: Submitter Id
characteristics: 1
characteristics: day
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: Protocols: To obtain synchronized populations, gravid adults were treated with hypochlorite and eggs were allowed to hatch in M9 overnight. ~ 1,600 L1 larvae per strain were plated on two 10 cm NG agar plates seeded with concentrated E. coli OP50 and cultured at 20 °C. At the L4-stage, 1,500 worms per strain were manually transferred to two E. coli OP50-seeded 10 cm NG agar plates supplemented with 20 µM FUDR to inhibit germ cell proliferation and progeny production. At day 1 of adulthood, worms were harvested by washing them off their plates with M9. After additional washing with M9 and RNAse-free water, worms were suspended in 1 ml Trizol, snap-frozen in liquid nitrogen and stored at -80 °C until RNA-extraction. RNA was extracted using Trizol and cleaned up with the Monarch® Total RNA Miniprep Kit or RNA Cleanup Kit (New England Biolabs, Ipswich, MA) according to the manufacturer’s instructions. Equal amounts of RNA extracted from three biological replicates were pooled for RNA-sequencing to mitigate batch effects. RNA library preparation was performed by Eurofins Genomics (Konstanz, Germany) using proprietary protocols and NEBNext Ultra II Directional RNA library preparation kit for Illumina.
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: egl-8(md1971) V
characteristics: genotype
characteristics: 20 °C, 20 µM FUDR from L4 onwards
characteristics: growth condition
characteristics: Caenorhabditis elegans
characteristics: organism
characteristics: Caenorhabditis elegans
characteristics: organism
characteristics: whole organism
characteristics: organism part
characteristics: Caenorhabditis elegans
characteristics: scientific_name
characteristics: hermaphrodite
characteristics: sex
characteristics: HMT035
characteristics: strain
characteristics: daf-2(+)_egl-8(-)
characteristics: title