characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-12-09T00:25:59Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-12-09T00:25:59Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110278161
characteristics: External Id
characteristics: University College London UK Dementia Research Institute
characteristics: INSDC center name
characteristics: 2022-12-09T00:25:59Z
characteristics: INSDC first public
characteristics: 2022-12-09T00:25:59Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12375775
characteristics: SRA accession
characteristics: E-MTAB-11918:Sample 12
characteristics: Submitter Id
characteristics: 6
characteristics: month
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: microglial cell
characteristics: cell type
characteristics: house mouse
characteristics: common name
characteristics: Protocols: Cell suspensions were filtered through a 70 μM cell strainer before mixing with Debris Removal Solution. Cells were centrifuged after (300 g for 10 min) and washed with ice-cold FACS buffer (PBS, 2 % FBS, 0.78 mM EDTA). After centrifugation, cells were incubated for 30 min at 4°C with FACS buffer containing Fc block (BD Biosciences) and primary antibody mix. Finally, right before loading the cells on the flow cytometer, cells were stained with DAPI (1:10000). Single cell suspensions were converted to barcoded Drop-seq libraries by using the Chromium Single Cell 3’ Library, Gel Bead & Multiplex Kit and Chip Kit (10X Genomics; Pleasanton, California, USA). cell libraries were prepared using 10X Genomics Chromium Next GEM Single Cell 3’ kit v3.1, according to the manufacturer’s instructions. As sorted PVMs and fibroblasts were in low abundance, we processed the whole sample, while for microglia cells we aimed for 5,000 cells per sample. Libraries were sequenced using an SP flowcell on an Illumina NovaSeq S6000 instrument, with sequencing parameters recommended by 10X Genomics, aiming for 40,000 reads per cell.
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: Alzheimer's disease
characteristics: disease
characteristics: AppNL-F/NL-F x Spp1KO/KO
characteristics: genotype
characteristics: CD45-int; CD11-int; CX3CR1-high
characteristics: immunophenotype
characteristics: Mus musculus
characteristics: organism
characteristics: Mus musculus
characteristics: organism
characteristics: hippocampus
characteristics: organism part
characteristics: App expression; Spp1 deficiency
characteristics: phenotype
characteristics: Mus musculus
characteristics: scientific_name
characteristics: male
characteristics: sex
characteristics: C57BL/6J
characteristics: strain
characteristics: Sample 12
characteristics: title