characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-15T00:26:26Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-15T00:26:26Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111436939
characteristics: External Id
characteristics: The Wadsworth Center/NYSDOH
characteristics: INSDC center name
characteristics: 2022-10-15T00:26:26Z
characteristics: INSDC first public
characteristics: 2022-10-15T00:26:26Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13530613
characteristics: SRA accession
characteristics: E-MTAB-12242:tylosin-3
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: Protocols: Cultures were filtered at 17 min post-treatment. 200 ml of cells were grown in LB medium until OD600~1.1. One set of cultures was left untreated, other sets were treated with 1 μg/ml tetracycline or 400 μg/ml tylosin. Monosomes were isolated as previously described (Smith et al 2019 biorXiv) Library was constructed as previously described (Smith et al 2019 biorXiv) with minor modification: 3’ linked oligonucleotide ligation reaction was carried overnight at 16oC. Circularized, reverse-transcribed cDNA was PCR-amplified for 6-8 cycles using primers containing Illumina sequencing indices
characteristics: description
characteristics: exponential phase
characteristics: developmental stage
characteristics: wild type genotype
characteristics: genotype
characteristics: LB medium
characteristics: growth condition
characteristics: Escherichia coli str. K-12 substr. MG1655
characteristics: organism
characteristics: Escherichia coli str. K-12 substr. MG1655
characteristics: organism
characteristics: whole organism
characteristics: organism part
characteristics: Escherichia coli str. K-12 substr. MG1655
characteristics: scientific_name
characteristics: MG1655
characteristics: strain
characteristics: tylosin-3
characteristics: title