characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-08T00:25:07Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-08T00:25:07Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111408297
characteristics: External Id
characteristics: Universitat Politecnica de Valencia
characteristics: INSDC center name
characteristics: 2022-10-08T00:25:07Z
characteristics: INSDC first public
characteristics: 2022-10-08T00:25:07Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13501962
characteristics: SRA accession
characteristics: E-MTAB-12205:Sample 25
characteristics: Submitter Id
characteristics: 52
characteristics: year
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: proliferative verrucous leukoplakia
characteristics: clinical information
characteristics: human
characteristics: common name
characteristics: Protocols: Collection of 37 samples from patients visited and treated at the Stomatology and Maxillofacial Surgery Department of the General University Hospital of Valencia. For all patients, two representative biopsies were taken from the same area of the lesions, including epithelium and the underlying connective tissue. One of each pair of specimens was analyzed with the routine histopathological methods to ensure that the observed lesions meet the histopathological criteria to establish the diagnosis together with the clinical data of each patient. The other sample was used for the RNAseq analysis. In this case, tissue samples were immediately frozen at -80 °C until their analysis. Samples were extracted using column-based DNA extraction method (E.Z.N.A. DNA kit and DNeasy Blood & Tissue Kit; Qiagen, Hilden, Germany) following manufacturer's instructions. cDNA synthesis and library generation were performed using the Illumina TruSeq RNA Library Prep Kit with PolyA selection for ribo depletion, following the manufacturer’s instructions.
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: oral mucosa leukoplakia
characteristics: disease
characteristics: LVP 47074
characteristics: individual
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: organism
characteristics: oral mucosa
characteristics: organism part
characteristics: Homo sapiens
characteristics: scientific_name
characteristics: female
characteristics: sex
characteristics: Sample 25
characteristics: title