characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-13T12:28:57Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-13T12:28:57Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111418858
characteristics: External Id
characteristics: WAGENINGEN UNIVERSITY
characteristics: INSDC center name
characteristics: 2022-10-13T12:28:57Z
characteristics: INSDC first public
characteristics: 2022-10-13T12:28:57Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: FAIR Data Station
characteristics: Metadata submission software
characteristics: ERS13512533
characteristics: SRA accession
characteristics: Pta_r47_2
characteristics: Submitter Id
characteristics: 1
characteristics: biosafety level
characteristics: In order to investigate the off-target mutations that occur in the cells during the process of recombineering when using each individual SSAP, we used the GenElute Bacterial Genomic DNA kit (Sigma-Aldrich) to isolate gDNA from two colonies of the next strains: (i) P. putida expressing pSEVA2514-R8-mutL, (ii) P. putida expressing pSEVA2514-R12-mutL, (iii) P. putida expressing pSEVA2514-R35-mutL, (iv) P. putida expressing pSEVA2514-R47-mutL, (v) P. taiwanensis expressing pSEVA2514-R47-mutL, (vi) P. fluorescens expressing pSEVA2514-R9-mutL, (vii) P. fluorescens expressing pSEVA2514-R47-mutL, (viii) P. aeruginosa expressing pSEVA6514-R8-mutL. Successful incorporation of an oligonucleotide mediating either the rpsL gene mutation in P. putida, P. taiwanensis and P. fluorescens, or the rpoB gene mutation in P. aeruginosa had been performed using one recombineering cycle in the aforementioned strains prior gDNA isolation. In the same way, but without undertaking any recombineering, we extracted the gDNA from one colony of each four wild type parental strains.
characteristics: observation_unit description
characteristics: Pta_r47_2
characteristics: observation_unit identifier
characteristics: P. taiwanensis expressing pSEVA2514-R47-mutL
characteristics: observation_unit name
characteristics: Pseudomonas taiwanensis
characteristics: organism
characteristics: Pseudomonas sp. VLB120
characteristics: organism
characteristics: UNLOCK_CORE
characteristics: packageIdentifier
characteristics: Core
characteristics: packageName
characteristics: P. taiwanensis expressing pSEVA2514-R47-mutL
characteristics: sample description
characteristics: Pta_r47_2
characteristics: sample identifier
characteristics: P. taiwanensis expressing pSEVA2514-R47-mutL
characteristics: sample name
characteristics: Pseudomonas sp. VLB120
characteristics: scientific_name
characteristics: P. taiwanensis
characteristics: scientific_name
characteristics: 69328
characteristics: tax_id
characteristics: P. taiwanensis expressing pSEVA2514-R47-mutL
characteristics: title