characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-11-01T00:15:39Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-11-01T00:15:39Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110696896
characteristics: External Id
characteristics: National Taiwan University Hospital
characteristics: INSDC center name
characteristics: 2022-11-01T00:15:39Z
characteristics: INSDC first public
characteristics: 2022-11-01T00:15:39Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: SINHCAF shRNA 1
characteristics: RNA interference
characteristics: ERS12794434
characteristics: SRA accession
characteristics: E-MTAB-12119:Sample 2
characteristics: Submitter Id
characteristics: 22
characteristics: year
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: NT2/D1
characteristics: cell_line
characteristics: germ cell
characteristics: cell_type
characteristics: human
characteristics: common name
characteristics: Protocols: NTERA2/D1 (NT2/D1) cell line was used in the experiment. shRNAs were transduced to NTERA2/D1 cells, targeting RFP as control and targeting SINHCAF as test, and selected by puromycin for 7 days. Cell pellettes are selected. Total RNA was extracted from cell lines using the Trizol reagent. 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: testicular embryonal carcinoma
characteristics: disease
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: organism
characteristics: testis
characteristics: organism part
characteristics: Homo sapiens
characteristics: scientific_name
characteristics: male
characteristics: sex
characteristics: Sample 2
characteristics: title