characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-11-21T16:00:06Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-12-19T00:24:58Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110196487
characteristics: External Id
characteristics: BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), Biodiscovery Institute, School of Life Sciences, The University of Nottingham
characteristics: INSDC center name
characteristics: 2022-11-21T16:00:06Z
characteristics: INSDC first public
characteristics: 2022-12-19T00:24:58Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12294162
characteristics: SRA accession
characteristics: E-MTAB-11897:Aspartate - 1
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: Protocols: Samples were collected at the early exponential growth phase (OD600 ~1) and treated with RNAprotect Bacteria Reagent (QIAGEN, Germany) according to the manufacturer instructions. C. necator H16 ΔmmsA123 ΔphaCAB colonies were used to inoculate tubes containing 5 ml of LB. Each tube was incubated at 30°C overnight with shaking at 200 rpm. Precultures were centrifugated at 8000 rpm for 3 min, washed twice and used to prepare 25 ml of culture at OD600 ~0.1 in a 250 ml baffled flask. 2%SGMM containing 4 g/l (NH4)2SO4 with and without 4 g/l aspartate was used as the culture medium. The flasks were incubated at 30°C with shaking at 200 rpm. Performed by GENEWIZ Performed by GENEWIZ
characteristics: description
characteristics: ΔmmsA1 ΔmmsA2 ΔmmsA3 ΔphaCAB
characteristics: genotype
characteristics: 2%SGMM containing 4 g/l (NH4)2SO4
characteristics: growth condition
characteristics: Cupriavidus necator H16
characteristics: organism
characteristics: Cupriavidus necator H16
characteristics: organism
characteristics: whole organism
characteristics: organism part
characteristics: Cupriavidus necator H16
characteristics: scientific_name
characteristics: Aspartate - 1
characteristics: title