characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-23T00:36:54Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-23T00:36:54Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111412526
characteristics: External Id
characteristics: MRC London Institute of Medical Sciences (LMS), Faculty of Medicine, Imperial College London
characteristics: INSDC center name
characteristics: 2022-10-23T00:36:54Z
characteristics: INSDC first public
characteristics: 2022-10-23T00:36:54Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13506191
characteristics: SRA accession
characteristics: E-MTAB-12217:E12-5-KO
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: house mouse
characteristics: common name
characteristics: Protocols: Hearts were isolated at E11.5, E12.5 and E14.5 stages. Atria and valves were removed, and ventricles were pooled (n = 3) and stored at -80°C until used. Total RNA was extracted and purified by using Trizol reactive (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. Libraries were generated using Stranded mRNA Library Prep Kit.
characteristics: description
characteristics: E12.5
characteristics: developmental stage
characteristics: Ccbe1 knockout
characteristics: genotype
characteristics: Mus musculus
characteristics: organism
characteristics: Mus musculus
characteristics: organism
characteristics: cardiac ventricle
characteristics: organism part
characteristics: Mus musculus
characteristics: scientific_name
characteristics: C57Bl/6
characteristics: strain
characteristics: E12-5-KO
characteristics: title