characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-12-13T00:34:14Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-12-13T00:34:14Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110255968
characteristics: External Id
characteristics: UGent
characteristics: INSDC center name
characteristics: 2022-12-13T00:34:14Z
characteristics: INSDC first public
characteristics: 2022-12-13T00:34:14Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12353585
characteristics: SRA accession
characteristics: E-MTAB-11905:SHEPplusDOX_SOX11
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: SHEP
characteristics: cell line
characteristics: neuroblast (sensu Vertebrata)
characteristics: cell type
characteristics: human
characteristics: common name
characteristics: Protocols: 0.5M cells were harvested for CUT&RUN. CUT&RUN was performed using Cutana pA/G-MNase (Epicypher, 15-1016) according to the manufacturer’s protocol. Cells were grown in RPMI1640 medium supplemented with 10% foetal bovine serum, 2mM L-Glutamine and 100 IU/ml penicillin/streptaviding at 37°C in a 5% CO2 humid atmosphere. SH-EP cells were treated with 0.1ug/ml doxycycline for 48h to induce SOX11 over expression. Retrieved genomic DNA was purified with the MinElute PCR purification kit and eluted in 10 mL of buffer EB. Sequencing libraries were prepared with the automated Swift 2S system.
characteristics: description
characteristics: neuroblastoma
characteristics: disease
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: organism
characteristics: bone marrow
characteristics: organism part
characteristics: SOX11 overexpression
characteristics: phenotype
characteristics: Homo sapiens
characteristics: scientific_name
characteristics: 0.1ug/ml doxycycline for 48h
characteristics: stimulus
characteristics: SHEPplusDOX_SOX11
characteristics: title