characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-09-30T00:20:53Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-09-30T00:20:53Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110590457
characteristics: External Id
characteristics: Wallenberg Center for Molecular Medicine (WCMM) Department of Biomedical and Clinical Sciences (BKV) Division of Molecular Medicine and Virology (MMV) Faculty of Medicine and Health Sciences, Linkoping University, Linkoping, Sweden
characteristics: INSDC center name
characteristics: 2022-09-30T00:20:53Z
characteristics: INSDC first public
characteristics: 2022-09-30T00:20:53Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12688232
characteristics: SRA accession
characteristics: E-MTAB-12075:hESC_CHIR_24h_C
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: hESC
characteristics: cell_line
characteristics: embryonic stem cell
characteristics: cell_type
characteristics: human
characteristics: common name
characteristics: Protocols: Cells collected from cell cultures, see growth protocol. hESCs were cultured in Essential 8TM medium (A1517001, ThermoFisher Scientific) in plates and flasks coated with 10 mg/ml Vitronectin (A14700, ThermoFisher Scientific). Cells were passaged by incubation in 0.5 mM EDTA-PBS (stock: 0.5 M EDTA, AM9260G, Thermo Fisher Scientific). Cells were treated with 10 mM CHIR99021 for 24 hours (n = 3 samples). Total RNA was extracted with the RNeasy Mini Kit (Cat. #74106, Qiagen). 500 ng of total RNA were processed with the Illumina Stranded mRNA Prep kit (Cat. #20040898, #20040896, #20040899, #20026121, Illumina) for library preparation. Messenger RNA (mRNA) was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. cDNA was then subjected to end repair, A-tailing, adapter ligation, size selection, amplification, and purification.
characteristics: description
characteristics: embryo stage
characteristics: developmental stage
characteristics: normal
characteristics: disease
characteristics: wild type genotype
characteristics: genotype
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: organism
characteristics: inner cell mass
characteristics: organism part
characteristics: Homo sapiens
characteristics: scientific_name
characteristics: hESC_CHIR_24h_C
characteristics: title