characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-09-28T16:20:35Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-09-28T16:20:35Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA10768608
characteristics: External Id
characteristics: Department of Agriculture, Food, Environment and Forestry (DAGRI), University of Florence, Italy
characteristics: INSDC center name
characteristics: 2022-09-28T16:20:35Z
characteristics: INSDC first public
characteristics: 2022-09-28T16:20:35Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS8420283
characteristics: SRA accession
characteristics: E-MTAB-11099:ID1667_P5F12-5_S4
characteristics: Submitter Id
characteristics: 9
characteristics: week
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: thale cress
characteristics: common name
characteristics: Protocols: Three leaves from each of 3 plants grown in the following experimental conditions were sampled and analyzed for F0 and F1 generation: 2.5 µM Cr (T1 (1 week) and T2 (3 weeks)); 5 µM Cr (T1 and T2), control (T1 and T2). Seeds of A. thaliana cv Col-0 were sown on peat Jyffy discs and kept in the dark at room temperature for three days. When plants reached 2 cm of total length, they were transferred in soil for 15 days and then in hydroponic culture (one plant per 500-mL pot containing Hoagland solution, (Hoagland & Arnon, 1950)) in a growth chamber (25°C for 16 hours of light at 120 µmol m-2s-1 and 22°C for 8 hours of dark, 60% of relative humidity). Plants were maintained one week in acclimation before the application of the stress. Chromium treatments (Cr(VI) supplied as K2Cr2O7) were the following: 1) 2.5 µM Cr (VI) until harvest after nine weeks of growth (chronic stress) and 5 µM Cr (VI) for three weeks and then recovering in Cr-free Hoagland solution until harvest. Five plants (biological replicates) were used for each of these stress conditions in addition to five control plants (grown in Hoagland in absence of Cr stress). Leaves were immediately frozen in liquid nitrogen and stored at -80 °C. RNA extraction was performed using 100 mg of leaves grounded with mortar and pestle in liquid nitrogen. The protocol of RNeasy Plant Mini kit (Qiagen) was followed and final elutions were performed using 50 µl of RNase-free water. Eighteen sequencing libraries were obtained following the procedure of Truseq Stranded mRNA Library Prep (Illumina) and using Truseq RNA Single Indexes.
characteristics: description
characteristics: rosette growth stage
characteristics: developmental stage
characteristics: Col-0
characteristics: ecotype
characteristics: F0
characteristics: generation
characteristics: wild type genotype
characteristics: genotype
characteristics: pool of 3 leaves from 3 plants
characteristics: individual
characteristics: Arabidopsis thaliana
characteristics: organism
characteristics: Arabidopsis thaliana
characteristics: organism
characteristics: leaf
characteristics: organism part
characteristics: Arabidopsis thaliana
characteristics: scientific_name
characteristics: ID1667_P5F12-5_S4
characteristics: title