characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-11-01T00:15:38Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-11-01T00:15:38Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110671120
characteristics: External Id
characteristics: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences (ICBFM SB RAS)
characteristics: INSDC center name
characteristics: 2022-11-01T00:15:38Z
characteristics: INSDC first public
characteristics: 2022-11-01T00:15:38Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12768698
characteristics: SRA accession
characteristics: E-MTAB-12088:Small_A549_24h_1
characteristics: Submitter Id
characteristics: 58
characteristics: year
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: A549
characteristics: cell_line
characteristics: epithelial cell
characteristics: cell_type
characteristics: human
characteristics: common name
characteristics: Protocols: A549 cells were maintained in Gibco DMEM/F12 medium supplemented with GlutaMAX and 10% FBS at 37°C with 5% CO2. Cells were mock infected or infected with influenza A/Puerto Rico/8/1934 virus (from the collection of Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian Federation) at a dose of 1 TID50/cell and incubated at 37°С for 24 h or 48 h. In this study influenza A/Puerto Rico/8/1934 was used to infect A549 cells. Mock-infected cells were included at analysis as control. Following the incubation period, the cell monolayer was washed twice with PBS and was directly lysed by adding phenol-containing LIRA reagent (Biolabmix, Russia). Small RNA (<200 nucleotide length) was extracted from cells using phenol/chloroform extraction with LRU RNA extraction kit (Biolabmix, Russia) according the manufacturer’s protocol and analyzed on a 1.5% TAE-agarose gel and using Bioanalyzer 2100 instrument (Agilent, USA) with an Agilent Small RNA kit. The quality of the Input RNA was assessed by the Agilent Bioanalyzer 2100 System and Agilent Small RNA kit (Agilent, USA). ~0.5 µg of enriched small RNA (<200 nucleotide length) was used for library construction. The construction of cDNA libraries according to a standard protocol using a NEBNext Multiplex Small RNA Library Prep Kit for Illumina (New England Biolabs, UK), as well as massive parallel sequencing on a NextSeq Illumina 500 platform, were conducted at the Institute of Fundamental Medicine and Biology, Kazan Federal University (Kazan, Russia). Libraries concentrations were quantified with the Qubit 2.0 Fluorometric Quantitation system using Qubit DNA HS Assay Kit (Thermo Fisher Scientific). The quality control analysis of libraries was assessed using the Agilent Bioanalyzer 2100 System and DNA High Sensitivity Chip (Agilent, USA).
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: adenocarcinoma
characteristics: disease
characteristics: wild type genotype
characteristics: genotype
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: organism
characteristics: lung epithelium
characteristics: organism part
characteristics: 1
characteristics: replicate
characteristics: Homo sapiens
characteristics: scientific_name
characteristics: male
characteristics: sex
characteristics: Small_A549_24h_1
characteristics: title