characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-12T09:19:55Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-20T16:34:51Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111349851
characteristics: External Id
characteristics: Brunel University London
characteristics: INSDC center name
characteristics: 2022-10-12T09:19:55Z
characteristics: INSDC first public
characteristics: 2022-10-20T16:34:51Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13447398
characteristics: SRA accession
characteristics: E-MTAB-12173:GX_MNX1_rep2
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: embryonic cell line
characteristics: cell_line
characteristics: embryonic stem cell
characteristics: cell_type
characteristics: house mouse
characteristics: common name
characteristics: Protocols: Mouse embryonic stem cells (mES) were detached from gelatinesed flasks by addition of trypsin. Cells were disassembled from 3-dimensional gastruloids (initiated from mES cells and biased towards hemato-endothelial cell production) at endopoint (216 h) using the dissociation agent TrypLE Express. Cells suspensions were quenched with medium and centrifuged at 400 x g for 5 minutes to pellet for subsequent extraction. Total RNA was extracted using Trizol (Invitrogen) and purified using Direct-zol RNA Purification Kits (Zymo Research). Library construction was performed by Novogene UK (Cambridge, UK). A minimum of 400 ng (20 ng/μl) of high-quality total RNA (extracted using Trizol, Invitrogen, and purified using Direct-zol RNA Purification Kits, Zymo Research) was supplied for sequencing. Novogene UK constructed cDNA libraries by purification of messenger RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification.
characteristics: description
characteristics: embryo stage
characteristics: developmental stage
characteristics: MNX1 overexpression
characteristics: genotype
characteristics: gastruloid culture
characteristics: growth condition
characteristics: Mus musculus
characteristics: organism
characteristics: Mus musculus
characteristics: organism
characteristics: embryoid body
characteristics: organism part
characteristics: Mus musculus
characteristics: scientific_name
characteristics: GX_MNX1_rep2
characteristics: title