characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-20T00:17:58Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-20T00:17:58Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111452015
characteristics: External Id
characteristics: Seoul National University
characteristics: INSDC center name
characteristics: 2022-10-20T00:17:58Z
characteristics: INSDC first public
characteristics: 2022-10-20T00:17:58Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13545414
characteristics: SRA accession
characteristics: E-MTAB-12258:eMutaT7[transition] cycle 0
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: Protocols: The cells (W3110) harboring a mutator plasmid (pDae079) and a target plasmid (pHyo182) were grown overnight in LB broth supplemented with 50 μg/mL cabenicillin and 35 μg/mL chloramphenicol (cycle #0). The overnight cultures (3.5 μl) were mixed with 350 μl of LB broth supplemented with 50 μg/mL carbenicillin, 35 μg/mL chloramphenicol, 0.2% arabinose, and 0.1mM IPTG in a 96-deep well plate (Bioneer, South Korea) and incubated at 37oC for 4 hours (cycle #1). The dilution-incubation cycle was repeated up to 20 times. Cells taken at cycle 0 (n = 1) and cycle 20 (n = 3) were grown in 15 mL of LB broth without arabinose and IPTG, and the target plasmids were extracted with Plasmid Miniprep Kit. The 3288-bp DNA fragments containing the pheS_A924G gene were amplified using primers 512 and 513 covering a region ranging from 999 bp upstream from T7 promoter to 1020 bp downstream from T7 terminator. Samples were then prepared with TruSeq Nano DNA Kit (TruSeq Nano DNA Sample Preparation Guide, Part # 15041110 Rev. D)
characteristics: description
characteristics: pDae079; pHyo182
characteristics: genotype
characteristics: LB broth containing eMutaT7[transition], LB 37'C 350ul for 0 hours
characteristics: growth condition
characteristics: Escherichia coli str. K-12 substr. MG1655
characteristics: organism
characteristics: Escherichia coli str. K-12 substr. MG1655
characteristics: organism
characteristics: whole organism
characteristics: organism part
characteristics: Escherichia coli str. K-12 substr. MG1655
characteristics: scientific_name
characteristics: W3110
characteristics: strain
characteristics: eMutaT7[transition] cycle 0
characteristics: title