characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-09-25T00:21:05Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-09-25T00:21:05Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA10368639
characteristics: External Id
characteristics: Latvian Biomedical Research and Study Centre
characteristics: INSDC center name
characteristics: 2022-09-25T00:21:05Z
characteristics: INSDC first public
characteristics: 2022-09-25T00:21:05Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS8023337
characteristics: SRA accession
characteristics: E-MTAB-11035:WT-1
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: osteosarcoma cell line
characteristics: cell line
characteristics: osteoblast
characteristics: cell type
characteristics: human
characteristics: common name
characteristics: Protocols: Cultivated cytoplasmic hybrid cells were collected and washed with PBS. All experimental workflow was performed in two biological replicates. Total RNA isolation with RNeasy Mini Kit (QIAGEN Science, MD, USA), according to the manufacturer's instructions. The integrity of the extracted RNA was evaluated by the RNA integrity number (RIN) within the Agilent 2100 Bioanalyzer system (Agilent, USA). Ribosomal RNA depletion was done with Low Input RiboMinus Eukaryote System v2 (Thermo Fisher Scientific, USA), by processing 500ng of total RNA from each sample. For cDNA library preparation we used the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific, USA).
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: normal
characteristics: disease
characteristics: m.13513G
characteristics: genotype
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: organism
characteristics: bone
characteristics: organism part
characteristics: Homo sapiens
characteristics: scientific_name
characteristics: WT-1
characteristics: title