characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-12T09:00:52Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-20T16:34:43Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110585939
characteristics: External Id
characteristics: Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen
characteristics: INSDC center name
characteristics: 2022-10-12T09:00:52Z
characteristics: INSDC first public
characteristics: 2022-10-20T16:34:43Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12683722
characteristics: SRA accession
characteristics: E-MTAB-12060:GR437
characteristics: Submitter Id
characteristics: 10
characteristics: week
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: house mouse
characteristics: common name
characteristics: Protocols: Mice were subject to a short-term fast of 4 hours before anesthesia with 2.5% isoflurane. A liver biopsy was taken from the right medial lobe and stored in RNAlater a -20 degree C until analysis. Raw sequencing data were processed using CASAVA v. 1.8 conversion software (Illumina). Total RNA was purified by an AllPrep DNA/RNA/ protein extraction kit (QIAgen) according to manufacturer’s instructions and qualified using a 2100 Bioanalyzer instrument (Agilent Genomics). RNA-sequencing was done using TruSeq Stranded Total RNA Library Prep Kit (Illumina) according to the manufacturer’s protocol: Ribosomal RNA was depleted using TruSeq Standed RNA Ribominus Gold (Illumina), and cDNA libraries were purified using AMPure XP Beads (Beckman). Following end-repair and poly(A)-tailing, adaptor ligation was performed with Truseq RNA single indexes set A/B (Illumina).
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: Gcgr knockout
characteristics: genotype
characteristics: Mus musculus
characteristics: organism
characteristics: Mus musculus
characteristics: organism
characteristics: liver
characteristics: organism part
characteristics: Mus musculus
characteristics: scientific_name
characteristics: male
characteristics: sex
characteristics: GR437
characteristics: title