characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-20T00:17:35Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-20T00:17:35Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110329943
characteristics: External Id
characteristics: EMBL-EBI
characteristics: INSDC center name
characteristics: 2022-10-20T00:17:35Z
characteristics: INSDC first public
characteristics: 2022-10-20T00:17:35Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12427028
characteristics: SRA accession
characteristics: E-MTAB-11946:UK29A
characteristics: Submitter Id
characteristics: 2.5 and 1 and 1
characteristics: year
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: dog
characteristics: common name
characteristics: Protocols: Mammalian liver samples were extracted post-mortem, perfused with PBS, and flash-frozen in liquid nitrogen. In almost all cases, tissues were prepared immediately post-mortem (typically within an hour) to maximize experimental quality, and were kept on ice until processed to minimize potential DNA degradation. Total genomic DNA was extracted from each sample with commercial reagents and following manufacturer guidelines (Qiagen, DNAEasy Blood&Tissue kit. Mammalian DNA was subjected to bisulfite conversion using Epimark CT conversion kit using Agilent and/or Epimark polymerase.
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: pool of 3 individuals
characteristics: individual
characteristics: Canis lupus familiaris
characteristics: organism
characteristics: Canis lupus familiaris
characteristics: organism
characteristics: liver
characteristics: organism part
characteristics: Canis lupus familiaris
characteristics: scientific_name
characteristics: male
characteristics: sex
characteristics: UK29A
characteristics: title