characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-11-14T15:53:10Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-11-14T15:53:10Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111451565
characteristics: External Id
characteristics: Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, United Kingdom
characteristics: INSDC center name
characteristics: 2022-11-14T15:53:10Z
characteristics: INSDC first public
characteristics: 2022-11-14T15:53:10Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13544965
characteristics: SRA accession
characteristics: E-MTAB-12256:M34_LV
characteristics: Submitter Id
characteristics: 36
characteristics: week
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: house mouse
characteristics: common name
characteristics: Protocols: Ten-week-old A/JOlaHsd female mice (Harlan Laboratories, UK) were used in this study, as approved by the Norwegian Animal Research Authority and following ACSM’s animal care standards. Ten week old A/JOlaHSD female mice (Harlan Laboratories, Loughborough, UK) were randomly divided into three groups and exposed to room air (n=10), CS (n=10) or CS plus HIIT (n=11). CS exposure (Research Cigarettes 3R4F, University of Kentucky, Lexington, KY, USA) was performed for six hours per day, five days per week for 20 weeks in an exposure chamber (TE-10, Teague Enterprises, Woodland, CA, USA). After CS exposure was completed, HIIT was performed five times per week for six weeks on a treadmill with 25 incline and gradually increasing speed as the training programme progressed. At sacrifice, the heart (left ventricle (LV) and right ventricle (RV)) and left lung were dissected, and immediately frozen in liquid nitrogen and stored at -80 for subsequent profiling. Total RNA was extracted from 91 (RV (n=29), LV (n=31) and lung (n=31)) mouse tissue samples, using RNeasy® Fibrous Tissue Mini Kit (Qiagen Sciences Inc., Germantown, MD, USA) and RNeasy Mini Kit (Qiagen) respectively, according to manufacturer´s instruction. RNA quantity, quality/integrity and purity were evaluated using Qubit (Thermo Fisher Scientific, Waltham, MA, USA), Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) and NanoDrop (Thermo Fisher). The libraries were quantified (KAPA Library Quantification Kit (Illumina/ABI Prism)), normalized and pooled. Quantitated libraries were further diluted to 2,4 nM and subjected to clustering by a cBot Cluster Generation System on one HiSeq4000 flowcells (Illumina Inc., San Diego, CA, USA), according to manufacturer´s instructions. Finally, single-end read sequencing was performed for 75 cycles on an Illumina HiSeq4000 instrument, by the manufacturer’s instructions (Illumina).
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: wild type genotype
characteristics: genotype
characteristics: M34
characteristics: individual
characteristics: Mus musculus
characteristics: organism
characteristics: Mus musculus
characteristics: organism
characteristics: heart left ventricle
characteristics: organism part
characteristics: Mus musculus
characteristics: scientific_name
characteristics: female
characteristics: sex
characteristics: control
characteristics: stimulus
characteristics: A/JOlaHsd
characteristics: strain
characteristics: M34_LV
characteristics: title