characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-09-24T00:21:09Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-09-24T00:21:09Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111351667
characteristics: External Id
characteristics: Bar-Ilan University
characteristics: INSDC center name
characteristics: 2022-09-24T00:21:09Z
characteristics: INSDC first public
characteristics: 2022-09-24T00:21:09Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13449214
characteristics: SRA accession
characteristics: E-MTAB-12178:WT BW25113
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: Protocols: E. coli trmD-knockout (KO) strains were made for MG1655, BW25113, and XAC background, maintained by expressing TrmD from pBAD promoter on a temperature-sensitive plasmid. The cells were depleted of the plasmid, pre-existing TrmD protein, and pre-existing m1G37-tRNA by growing in liquid LB medium without the inducer arabinose, at non-permissive temperature for multiple cycles. The resultant depleted cell cultures were streaked out and grown on an LB plate without arabinose at non-permissive temperature 43C, and suppressor colonies were isolated. Distinct colonies were streak-purified, and genomic DNA was prepared by phenol extraction from an overnight cell culture. Sequencing library was prepared using Nextera XT DNA Library Prep Kit (Illumina).
characteristics: description
characteristics: wild type genotype
characteristics: genotype
characteristics: Escherichia coli BW25113
characteristics: organism
characteristics: Escherichia coli BW25113
characteristics: organism
characteristics: Escherichia coli BW25113
characteristics: scientific_name
characteristics: BW25113
characteristics: strain
characteristics: WT BW25113
characteristics: title