characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-20T00:17:32Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-20T00:17:32Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110188675
characteristics: External Id
characteristics: BioMed X GmbH
characteristics: INSDC center name
characteristics: 2022-10-20T00:17:32Z
characteristics: INSDC first public
characteristics: 2022-10-20T00:17:32Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12287478
characteristics: SRA accession
characteristics: E-MTAB-11791:Syn_Bio_026_2
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: human
characteristics: common name
characteristics: Protocols: For protocol 1, we followed the method by Donlin L et al. for dissociation of cryopreserved synovium (steps 1-5): 1. Fresh tissue fragments, obtained by synovial biopsy were minced into small fragments (~1 mm3) using a scalpel. 2. Minced fragments were transferred to U bottom tubes containing a digestion mix ( RPMI culture medium with 100 μg/ml Liberase™ TL (Roche) and 100 μg/ml DNase I (Roche)). 3. Tissues were dissociated with a combination of enzymatic digestion (37°C for 30min) and mechanical disruption (16G syringe needle) under continuous stirring with a magnetic stir bar. 4. The mechanical disruption was performed 15 minutes after enzymatic digestion start by gently passing the samples through a 16G syringe needle (10x). 5. Enzymatic digestion was stopped with RPMI, containing 10% FCS. Thereafter, red blood cells in dissociated synovial cell pellets were lysed using Red Cell Lysis buffer (Roche), according to the manufacturer's instructions and cells were washed with PBS. Single cell 3´protocol (10x Genomics), v3.0/v3.1
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: arthritis
characteristics: disease
characteristics: 26
characteristics: individual
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: organism
characteristics: synovial membrane
characteristics: organism part
characteristics: Homo sapiens
characteristics: scientific_name
characteristics: female
characteristics: sex
characteristics: Syn_Bio_026_2
characteristics: title