characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-15T00:26:24Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-15T00:26:24Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111411857
characteristics: External Id
characteristics: Anatomy and Developmental Biology Monash Biomedical Discovery Institute Monash University
characteristics: INSDC center name
characteristics: 2022-10-15T00:26:24Z
characteristics: INSDC first public
characteristics: 2022-10-15T00:26:24Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13505522
characteristics: SRA accession
characteristics: E-MTAB-12214:MS1447
characteristics: Submitter Id
characteristics: 14
characteristics: month
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: house mouse
characteristics: common name
characteristics: Protocols: Tissue samples were obtained from the proximal small intestine using a scalpel blade. DNA was extracted using the AllPrep DNA RNA Protein Minikin We generated single base resolution DNA methylation data using the Ovation RRBS Methyl-Seq System 1-16 (Tecan Life Sciences) with 100ng of input DNA. This approach employs the MspI restriction enzyme to digest DNA at the CCGG motif, which is highly enriched in CpG dense regions of the genome. This generates a library of small (>300bp) fragments that are rich in CpG content. Sequencing libraries are generated from these fragments, bisulphite converted and sequenced.
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: wild type genotype
characteristics: genotype
characteristics: MS1447
characteristics: individual
characteristics: Mus musculus
characteristics: organism
characteristics: Mus musculus
characteristics: organism
characteristics: small intestine
characteristics: organism part
characteristics: proximal small intestine
characteristics: sampling site
characteristics: Mus musculus
characteristics: scientific_name
characteristics: male
characteristics: sex
characteristics: C57BL/6J
characteristics: strain
characteristics: MS1447
characteristics: title