characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-12-13T00:34:12Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-12-13T00:34:12Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110191395
characteristics: External Id
characteristics: UGent
characteristics: INSDC center name
characteristics: 2022-12-13T00:34:12Z
characteristics: INSDC first public
characteristics: 2022-12-13T00:34:12Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12289073
characteristics: SRA accession
characteristics: E-MTAB-11883:NGP_siSOX11_rep2
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: NGP
characteristics: cell line
characteristics: neuroblast (sensu Vertebrata)
characteristics: cell type
characteristics: human
characteristics: common name
characteristics: Protocols: Cell lines were harvested, centrifuged and stored at -80°C. Cells were grown in RPMI1640 medium supplemented with 10% foetal bovine serum (FBS), 2 mM L-Glutamine and 100 IU/ml penicillin/streptavidin at 37°C in a 5% CO2 humid atmosphere Cells were seeded in 6-well tissue culture plates 24 hours prior to transfection. 100nM of siRNA non-targeting control (siRNA NTC, D-001810-10-05) or siRNA SOX11 (L-017377-01-0005, Dharmacon) were transiently transfected using DharmaFect 2 (Thermo Fisher Scientific) according to the manufacturer's guidelines. For nucleofection, cells were nucleofected with 100 nM of the above described siRNA NTC and siRNA SOX11 using the Neon Transfection System (Thermo Fisher Scientific) and subsequently seeded in 6-well or T25 tissue culture plates. Total RNA was extracted using miRNeasy kit (Qiagen) according to manufacturer's instructions, including on-column DNase treatment, and concentration was determined with the Nanodrop (Thermo Scientific). CLB-GA samples were prepared using the TruSeq Stranded mRNA Sample Prep Kit from Illumina. And NGP samples were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit from Illumina.
characteristics: description
characteristics: neuroblastoma
characteristics: disease
characteristics: wild type genotype
characteristics: genotype
characteristics: lung
characteristics: metastatic site
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: organism
characteristics: brain
characteristics: organism part
characteristics: SOX11 knockdown by siRNA
characteristics: rna interference
characteristics: Homo sapiens
characteristics: scientific_name
characteristics: NGP_siSOX11_rep2
characteristics: title