characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-08T00:25:07Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-08T00:25:07Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111406350
characteristics: External Id
characteristics: The Wadsworth Center/ NYSDOH
characteristics: INSDC center name
characteristics: 2022-10-08T00:25:07Z
characteristics: INSDC first public
characteristics: 2022-10-08T00:25:07Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13500222
characteristics: SRA accession
characteristics: E-MTAB-12211:yjhX167-3
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: Protocols: Colonies selected from mutant screen for overnight cultures grown in LB at 37C. Bacterial cultures were grown at 37 °C in LB medium. 100 μl of an overnight culture was washed and plated on M9+0.2% lactose agar. Spontaneous survivors identified by whole genome sequencing Genomic DNA was prepared from overnight culture using a DNeasy Blood and Tissue Kit (Qiagen). A DNA library was prepared using a Nextera kit (Illumina).
characteristics: description
characteristics: yjhX167-3
characteristics: genotype
characteristics: grown overnight
characteristics: growth condition
characteristics: Escherichia coli str. K-12 substr. MG1655
characteristics: organism
characteristics: Escherichia coli str. K-12 substr. MG1655
characteristics: organism
characteristics: Escherichia coli str. K-12 substr. MG1655
characteristics: scientific_name
characteristics: MG1655
characteristics: strain
characteristics: yjhX167-3
characteristics: title