characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-16T00:33:17Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-16T00:33:17Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111437444
characteristics: External Id
characteristics: Department of Human Physiology and Pathophysiology, School of Medicine, University of Warmia and Mazury in Olsztyn
characteristics: INSDC center name
characteristics: 2022-10-16T00:33:17Z
characteristics: INSDC first public
characteristics: 2022-10-16T00:33:17Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13531112
characteristics: SRA accession
characteristics: E-MTAB-12252:Sample 5
characteristics: Submitter Id
characteristics: 32
characteristics: week
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: house mouse
characteristics: common name
characteristics: Protocols: Mice were sacrificed with a mixture of ketamine (300mg/kg, Narketan®, Vétoquinol Biowet, Poland) and xylazine (30mg/kg, VetaXyl®, Vet-Agro, Poland). Lumbar spinal cord were collected and snap‐frozen in liquid nitrogen and stored at −80°C for further analysis. Total RNA was extracted from lumbar spinal cord neuromere harvested from 32-weeks-old mice. RNA was isolated using RNeasy® Plus Universal Mini Kit and QIAzol® Lysis Reagent (both Qiagen, USA), according to manufacturer’s recommendations. Subsequently, RNA integrity (RIN), quality and quantity were evaluated with microcapillary electrophoresis (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA). Only samples with RIN above 8 were used for further analysis. For library construction, DNA/RNA is extracted from a sample. After performing quality control (QC), qualified samples proceed to library construction. The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, \tagmentation\ combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified.
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: diabetes type 1
characteristics: disease
characteristics: wild type genotype
characteristics: genotype
characteristics: 72
characteristics: individual
characteristics: Mus musculus
characteristics: organism
characteristics: Mus musculus
characteristics: organism
characteristics: lumbar spinal cord
characteristics: organism part
characteristics: Mus musculus
characteristics: scientific_name
characteristics: male
characteristics: sex
characteristics: C57BL/6
characteristics: strain
characteristics: Sample 5
characteristics: title
characteristics: STZ treatment
characteristics: treatment