characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-07T00:45:12Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-07T00:45:12Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111438628
characteristics: External Id
characteristics: Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
characteristics: INSDC center name
characteristics: 2022-10-07T00:45:12Z
characteristics: INSDC first public
characteristics: 2022-10-07T00:45:12Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13532050
characteristics: SRA accession
characteristics: E-MTAB-12181:Sample 146
characteristics: Submitter Id
characteristics: 9
characteristics: week
characteristics: age
characteristics: ArrayExpress
characteristics: broker name
characteristics: house mouse
characteristics: common name
characteristics: Protocols: Skin tissue was dissected from 9 week old female F1 offspring of matings between CAST/EiJ and C57BL/6J mice. Cells were dissociated from skin as described in Joost et al., or using GentleMACS (Miltenyi Biotec); with both methods giving similar cell yields and viability. Briefly, for the GentleMACS method, dorsal skin was cut and minced into small pieces (approximately 1x1mm) and incubated in HBSS (Sigma) + 0.04% BSA (Sigma) + 0.2% Collagenase Ia (Sigma) at 37°C for 60 minutes with occasional agitation. Thereafter this slurry was processed on a GentleMACS (Miltenyi Biotec) with 2x Program D, cell-strained (70um) and washed. Residual tissue was further treated with HBSS + 0.05% Trypsin-EDTA (Sigma) at 37°C for 15 minutes and processed likewise. For the Joost et al method, GentleMACS dissociation was substituted with manual disaggregation by smashing tissue fragments against a cell strainer with the piston from a 5 mL syringe Cell lysis during sorting by FACS. Cells were subjected to the Smart-seq2 protocol with no modification.
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: wild type genotype
characteristics: genotype
characteristics: 1
characteristics: individual
characteristics: Mus musculus
characteristics: organism
characteristics: Mus musculus
characteristics: organism
characteristics: skin
characteristics: organism part
characteristics: dorsal skin
characteristics: sampling site
characteristics: Mus musculus
characteristics: scientific_name
characteristics: female
characteristics: sex
characteristics: C57BL/6 x CAST/EiJ
characteristics: strain
characteristics: Sample 146
characteristics: title