characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-31T00:16:50Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-31T00:16:50Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111378967
characteristics: External Id
characteristics: University of Salerno
characteristics: INSDC center name
characteristics: 2022-10-31T00:16:50Z
characteristics: INSDC first public
characteristics: 2022-10-31T00:16:50Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13475952
characteristics: SRA accession
characteristics: E-MTAB-12193:Input_NAM_Rep2
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: Protocols: C. albicans cultures were cross-linked with 1% formaldehyde for 15 minutes at room temperature with gentle shaking. The reaction was quenched with 125 mM glycine and cells wer havested by centrifugation. Budding yeast cell culture was diluted at a cell density of 10^5 cells/mL in YPD and incubated at 25°C for 28 h with or without 10 mM NAM. ChIP was performed as previusly described (Mawer, J., Massen, J., Reichert, C., Grabenhorst, N., Mylonas, C., and Tessarz, P. (2021). Nhp2 is areader of H2AQ105me and part of a network integrating metabolism with rRNA synthesis.EMBO reports, 22(10), e52435. doi: 10.15252/embr.202152435) except for cell lysis that was carried out by using a cryogenic freezer mill (SPEX SamplePrep 6970EFM Freezer/Mill, München, Germany). DNA from Input and ChIP samples was purified with Macherey-Nagel NucleoSpin Gel and PCR Clean-Up Kit. Libraries were generated from H3K56ac and input following a previously published protocol (Ford, E., Nikopoulou, C., Kokkalis, A., and Thanos, D. (2014). A method for generating highly multiplexed ChIP-seq libraries. BMC research notes, 7, 312. doi: 10.1186/1756-0500-7-312).
characteristics: description
characteristics: Candida albicans
characteristics: organism
characteristics: Candida albicans
characteristics: organism
characteristics: whole organism
characteristics: organism part
characteristics: Candida albicans
characteristics: scientific_name
characteristics: Input_NAM_Rep2
characteristics: title