characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-20T00:17:58Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-20T00:17:58Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111439163
characteristics: External Id
characteristics: e-NIOS Applications PC
characteristics: INSDC center name
characteristics: 2022-10-20T00:17:58Z
characteristics: INSDC first public
characteristics: 2022-10-20T00:17:58Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13532585
characteristics: SRA accession
characteristics: E-MTAB-12224:1-2D-T47D-control
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: T-47D
characteristics: cell_line
characteristics: human
characteristics: common name
characteristics: Protocols: Two human epithelial BC cell lines, authenticated in July 2018 by the short tandem repeat DNA profiling, were used in this study. JIMT-1 (DSMZ no.: ACC 589). Cells were cultivated at 37°C in a humidified atmosphere and 5% CO2in high-glucose (4.5 g/l) Dulbecco’s modified Eagle medium (DMEM, PAA Laboratories GmbH, Austria) supplemented with 10% fetal bovine serum (FBS, Biochrom AG, Germany), 10 μg/ml gentamicin (Sandoz, Germany) and 2 mM glutamine (PAA Laboratories GmbH). RNeasy Mini Kit (Qiagen, Germany) was used to isolate total RNA from cell pellets, using the On-column DNase digestion step with the RNAse-Free DNase Set (Qiagen). Extracted RNA quality and quantity were evaluated using NanoDrop® ND-1000 spectrophotometer (Thermo Fisher Scientific). BGI DNBseq library construction
characteristics: description
characteristics: breast ductal adenocarcinoma
characteristics: disease
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: organism
characteristics: mammary gland
characteristics: organism part
characteristics: luminal A subtype
characteristics: phenotype
characteristics: Homo sapiens
characteristics: scientific_name
characteristics: 1-2D-T47D-control
characteristics: title