characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-09-29T00:17:16Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-09-29T00:17:16Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA110177370
characteristics: External Id
characteristics: INSERM Gustave Roussy institute
characteristics: INSDC center name
characteristics: 2022-09-29T00:17:16Z
characteristics: INSDC first public
characteristics: 2022-09-29T00:17:16Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS12276178
characteristics: SRA accession
characteristics: E-MTAB-11033:TSMR89
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: Induced pluripotent stem cell
characteristics: cell line
characteristics: CD34-negative, CD41-positive, CD42-positive megakaryocyte cell
characteristics: cell type
characteristics: human
characteristics: common name
characteristics: Protocols: Cells were labeled with APC, BV421 and PE-conjugated antibodies against CD41a, CD42b and CD33, respectively. In order to obtain a highly purified megakaryocytes, CD33+ cells were removed from the double positive CD41, CD42 population during cell sorting. After sorting, the purity was verified and corresponded to 98-100% of CD41+, CD42+, CD33-. Total RNA was extracted using RNA/DNA/Protein Purification Plus Kit (Proteigene, St Marcel, France). cDNA libraries were synthesized from 250 ng total RNA using TruSeq Strandard mRNA Kit (Illumina). Libraries were verified for their amount and quality by capillary electrophoresis using a 2100 Bioanalyzer (Agilent Technologies).
characteristics: description
characteristics: embryo stage
characteristics: developmental stage
characteristics: Down syndrome
characteristics: disease
characteristics: Disomic with GATA1s mutation MPL mutation
characteristics: genotype
characteristics: Homo sapiens
characteristics: organism
characteristics: Homo sapiens
characteristics: organism
characteristics: Non applicable
characteristics: organism part
characteristics: Homo sapiens
characteristics: scientific_name
characteristics: TSMR89
characteristics: title