characteristics: ERC000011
characteristics: ENA-CHECKLIST
characteristics: 2022-10-01T16:26:39Z
characteristics: ENA-FIRST-PUBLIC
characteristics: 2022-10-01T16:26:39Z
characteristics: ENA-LAST-UPDATE
characteristics: SAMEA111280578
characteristics: External Id
characteristics: Laboratory of Cell Signalling, Institute of Microbiology of the Czech Academy of Sciences
characteristics: INSDC center name
characteristics: 2022-10-01T16:26:39Z
characteristics: INSDC first public
characteristics: 2022-10-01T16:26:39Z
characteristics: INSDC last update
characteristics: public
characteristics: INSDC status
characteristics: ERS13378175
characteristics: SRA accession
characteristics: E-MTAB-12132:control_R2
characteristics: Submitter Id
characteristics: ArrayExpress
characteristics: broker name
characteristics: MDCK
characteristics: cell_line
characteristics: epithelial cell
characteristics: cell_type
characteristics: dog
characteristics: common name
characteristics: Protocols: Parental MDCK cell line (FRT), ERK2 overexpressing MDCK cell line (ERK2) and MDCK cell line expressing conditionally active ΔRaf1:ER protein (Raf1:ER) were grown in triplicates for parental cells and in duplicates for ERK2 and Raf1:ER cells. Cells were grown for 48 h in DMEM supplemented with 10% FBS. Raf1:ER cells were stimulated by 4-Hydroxytamoxifen for 24 h prior to harvesting to activate the ERK pathway. Cells at ~ 50% confluency were lysed in RLT buffer, RNA was extracted by RNeasy mini kit and RNA integrity was verified using Agilent 2100 Bioanalyzer. Poly(A) enriched RNA was subjected to RNA-sequencing by the Illumina platform. Processing of poly(A) enriched RNA and RNA-sequencing was performed on a commercial basis by SEQme company (https://www.seqme.eu/). 15 million single-end 75nt reads per sample were obtained.
characteristics: description
characteristics: adult
characteristics: developmental stage
characteristics: normal
characteristics: disease
characteristics: wild type genotype
characteristics: genotype
characteristics: Canis lupus familiaris
characteristics: organism
characteristics: Canis lupus familiaris
characteristics: organism
characteristics: kidney
characteristics: organism part
characteristics: wild type
characteristics: phenotype
characteristics: 2
characteristics: replicate
characteristics: control
characteristics: sample group
characteristics: Canis lupus familiaris
characteristics: scientific_name
characteristics: control_R2
characteristics: title