characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-26T00:16:52Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-26T00:16:52Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110702373
characteristics.characteristic: External Id
characteristics.text: Philipps-Universitat Marburg Center for Synthetic Microbiology (Synmikro) Becker lab
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-26T00:16:52Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-26T00:16:52Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12799911
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12126:Rm1021 ECF/anti-σ-free strain with empty vector rep3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Rm1021 ECF/anti-σ-free strain derivatives were grown to an OD600 of 0.1-0.3 in MOPS buffered minimal medium with gentamicin (6 µg/mL). The OD600 was adjusted to 0.1 and IPTG was added at a final concentration of 500 µM. Strains were grown for ~ 8-9 hours until cell density reached an OD600 of 0.8 and collected by centrifugation at 10,000 rcf for 5 min. Cell pellets were frozen in liquid nitrogen and kept at -80 °C. Assays were performed in triplicates for each strain. For total RNA isolation, the Qiagen miRNeasy Kit was used starting from a pellet of 8 mL cultured cells. The cell pellet was resuspended in 1 mL QIAzol lysis reagent and homogenized in a FastPrep® sample preparation system using Lysing Matrix B containing 0,1 mm silica beads (MP biomedicals) and the following settings: 3x 6500 rpm for 20 sec, 15 sec break. The supernatant was transferred into a new tube and RNA isolation was performed according to the manufacturer’s instruction including the optional on column DNaseI digestion. rRNA depletion was conducted with the Illumina Ribo-Zero rRNA removal Kit (Bacteria). The cDNA library was prepared using the NEB Ultra directional Kit.
characteristics.characteristic: description
characteristics.text: vegetative growth
characteristics.characteristic: developmental stage
characteristics.text: Rm1021 Δecf/anti-σ pSRKGm [m]
characteristics.characteristic: genotype
characteristics.text: Sinorhizobium meliloti
characteristics.characteristic: organism
characteristics.text: Sinorhizobium meliloti
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Sinorhizobium meliloti
characteristics.characteristic: scientific_name
characteristics.text: Rm1021
characteristics.characteristic: strain
characteristics.text: Rm1021 ECF/anti-σ-free strain with empty vector rep3
characteristics.characteristic: title