characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-12-21T00:27:24Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-12-21T00:27:24Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111539409
characteristics.characteristic: External Id
characteristics.text: Laboratory of Pharmaceutical Microbiology Department of Pharmaceutical Sciences Ghent University
characteristics.characteristic: INSDC center name
characteristics.text: 2022-12-21T00:27:24Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-12-21T00:27:24Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13649473
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12357:Sample 3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Cell material was collected from the surface of a tryptic soy agar plate with a sterile cotton swab. The cell material was suspended in 200 µl Tris-EDTA and added to 500 µl of lysis buffer (50 mM Tris-HCl [pH 8], 70 mM EDTA [pH 8], 1% sodium dodecyl sulfate), containing 0.5mg/ml pronase and acid-washed glass beads in a 2 ml micro centrifuge tube. After vigorous vortexing for 10 s, the samples were incubated at 37°C for 30 min. Then 400 µl of saturated ammonium acetate was added and the samples were vortexed for 10 s prior to centrifugation (2 min, 13,000 rpm). 600 µl of chloroform was added and the samples were vortexed for another 10 s. The phases were separated by centrifugation (5 min, 13,000 rpm) and 400 µl of the top aqueous phase was transferred to a DNase-free 1.5 ml micro centrifuge tube containing 1 ml of 100% ethanol. After centrifugation (5 min, 13,000 rpm) the pellet was washed with 500 µl of 70% ethanol and dried in a SpeedVac for 10 min. Finally, the extracted DNA was dissolved in 100 µl of low EDTA-Tris buffer (0.1 mM EDTA, 10 mM Tris-HCl [pH 8.5]) containing 0.5mg/ml RNase and was incubated at 37°C for 1 h. NEBNext Ultra II library prep
characteristics.characteristic: description
characteristics.text: Acinetobacter baumannii
characteristics.characteristic: organism
characteristics.text: Acinetobacter baumannii
characteristics.characteristic: organism
characteristics.text: MIC fosmidomycin derivative CC366 = 32 µg/ml
characteristics.characteristic: phenotype
characteristics.text: Acinetobacter baumannii
characteristics.characteristic: scientific_name
characteristics.text: G2066
characteristics.characteristic: strain
characteristics.text: Sample 3
characteristics.characteristic: title