characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-31T00:19:53Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-31T00:19:53Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111385587
characteristics.characteristic: External Id
characteristics.text: Systems Biology of Inflammation, the German Rheumatism Research Centre Berlin, a Leibniz Institute (DRFZ)
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-31T00:19:53Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-31T00:19:53Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13482565
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12203:Std_CD4_SLE_0102
characteristics.characteristic: Submitter Id
characteristics.text: 56
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: helper T cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Lithium Heparin (LiHep)-anticoagulated blood was withdrawn from a total of 24 healthy donors, 25 SLE patients and 23 pSS patients. PBMCs were isolated from LiHep-anticoagulated blood. The collected mononuclear cells were washed twice with PBS. cells were rested for 30 min at 37°C in ImmunoCult-XF T cell expansion medium (Stemcell). 96-well plate were coated with 0.1 µg/ml anti-CD3 (OKT3, eBioscience) for 2h at 37°C or PBS as control, washed two times with PBS, blocked with PBS/1% BSA for 30 min and washed two times with PBS. 1x106 PBMCs were seeded per well and incubated at 37°C and 5% CO2 in a humidified incubator for 18hours. The PicoPure RNA Kit (Thermo Fisher) was used for purification of total RNA from cells. Library generation has been completed using the SMARTer cDNA Synthesis and Nextera XT chemistry. With 500pg total RNA, cDNA pools were prepared using the SMART-Seq v4 Kit (Clontech Laboratories) to generate full-length cDNA from total RNA.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: systemic lupus erythematosus
characteristics.characteristic: disease
characteristics.text: CD4+; CD127+; CD25-
characteristics.characteristic: immunophenotype
characteristics.text: SLE_0102
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: Std_CD4_SLE_0102
characteristics.characteristic: title