characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-01T00:28:35Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-01T00:28:35Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111280431
characteristics.characteristic: External Id
characteristics.text: Max Delbrueck Center for Molecular Medicine Berlin Institute For Medical Systems Biology Free University of Berlin
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-01T00:28:35Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-01T00:28:35Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13378028
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12129:NAGr- 3
characteristics.characteristic: Submitter Id
characteristics.text: 68
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: 3 normal adrenal gland samples were collected from tissue surrounding endocrine inactive adenoma bearing patients. 3 normal adrenal gland samples were collected from adrenalectomies performed on patients with renal cell carcinoma.7 cortisol producing adenoma samples were collected from patients with hypercortisolism. 5 endocrine inactive adenoma samples were collected from patients with inactive adrenal tumors. Samples were snap-frozen immediately after surgery. Nuclei were isolated from these snap frozen tissues using dounce homogenization and were purified via flow cytometry. The inDrop (1CellBio) workflow was followed to yield barcoded single-nuclei cDNA libraries. Briefly, cells were encapsulated hydrogel beads, droplet oil, RT/lysis mixture and the nuclei. The encapsulated cells were then exposed to UV light, releasing photocleavable primers from hydrogel beads. Subsequently, reverse transcription was performed and the beads filtered out. The RT product was digested using ExoI and HinFI and purified using AMpure XP beads. Then, the second strand cDNA synthesis and in vitro transcription steps were carried out. Next, the resulting RNA molecules were fragmented and a second reverse transcription step was carried out using random hexamers. The cDNA was then purified using Ampure XP beads and quantified via qPCR.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: renal cell carcinoma
characteristics.characteristic: disease
characteristics.text: NAGr- 3
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: adrenal gland
characteristics.characteristic: organism part
characteristics.text: normal tissue adjacent to tumor
characteristics.characteristic: sampling site
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: NAGr- 3
characteristics.characteristic: title