characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-12-31T00:58:37Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-12-31T00:58:37Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111350864
characteristics.characteristic: External Id
characteristics.text: St. John's Medical College Hospital, Bangalore
characteristics.characteristic: INSDC center name
characteristics.text: 2022-12-31T00:58:37Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-12-31T00:58:37Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13448411
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12162:SiHa-N 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: SiHa
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: SiHa cells were cultured in a CO2 incubator using DMEM supplemented with 10% FBS. RNA was extracted using the TRIzol method. The mRNA was subjected to first strand synthesis, followed by second strand synthesis. The double stranded cDNA was purified using HighPrep magnetic beads. The purified cDNA was end-repaired, adenylated and ligated to Illumina multiplex barcode adapters as per NEBNext® Ultra™ Directional RNA Library Prep Kit protocol.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: squamous cell carcinoma
characteristics.characteristic: disease
characteristics.text: pcDNA3 empty vector
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: uterine cervix
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: SiHa-N 1
characteristics.characteristic: title