characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-01T00:28:12Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-01T00:28:12Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110177358
characteristics.characteristic: External Id
characteristics.text: Laboratory of Molecular Infection Medicine Sweden (MIMS), Department of molecular biology, Umea university
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-01T00:28:12Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-01T00:28:12Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12276166
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11862:P3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: B cell
characteristics.characteristic: cell type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: CD19+ B cells from tumor-draining lymph nodes (S), non-draining lymph nodes (N), and peripheral blood (P) were positively isolated using a Dynabeads Pan B cell kit (Thermo scientific # 11143D). Isolated B cells were washed and counted. Nucleospin XS kit (Macherey-Nagel # 740902.50) was used for total RNA isolation according to the manufacturer’s instructions. 1 - 10 ng of total RNA was used for the generation of IgM/IgG heavy and light chain libraries of V(D)J variable regions of BCR transcripts employing the SMARTer Human BCR IgG IgM H/K/L Profiling Kit (Takara Bio, #634466).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: prostate cancer
characteristics.characteristic: disease
characteristics.text: T3a
characteristics.characteristic: disease staging
characteristics.text: gleason score 9
characteristics.characteristic: gleason score
characteristics.text: CD19 positive
characteristics.characteristic: immunophenotype
characteristics.text: 3
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: prostate specific antigen score 8.6
characteristics.characteristic: prostate specific antigen score
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: P3
characteristics.characteristic: title