characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-06T00:32:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-06T00:32:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112165288
characteristics.characteristic: External Id
characteristics.text: Jonathon C.O. Mifsud, Vincenzo A. Costa, Mary E. Petrone, Ezequiel M. Marzinelli, Edward C. Holmes, Erin Harvey
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-06T00:32:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-06T00:32:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14275033
characteristics.characteristic: SRA accession
characteristics.text: ena-SAMPLE-TAB-28-11-2022-03:15:05:694-79142
characteristics.characteristic: Submitter Id
characteristics.text: The tunicate Botrylloides leachii was collected by divers wearing surgical gloves at 0.5-3m depth at the pier-pilings in Chowder Bay, Sydney, Australia (site description in Marzinelli (2012)), on 24 November, 2021. Sections of colonies were detached from the substratum using sterile tweezers, which were rinsed in 80% ethanol between samples, and brought to the surface, where they were placed in sterile cryogenic tubes. Samples were stored in liquid nitrogen on site and then transferred to a -80�C freezer until extraction. Total RNA was extracted using the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) as previously described in (Geoghegan et al. 2021). These libraries were constructed using the Truseq Total RNA Library Preparation Protocol (Illumina). Host ribosomal RNA (rRNA) was depleted with the Ribo-Zero Plus Kit (Illumina) and paired-end sequencing (150 bp) was carried out on the NovaSeq 500 platform (Illlumina). Library construction and metatranscriptomic sequencing were performed by the Australian Genome Research Facility (AGRF).
characteristics.characteristic: description
characteristics.text: Australia
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: Chowder Bay, Sydney
characteristics.characteristic: geographic location (region and locality)
characteristics.text: Botrylloides leachii
characteristics.characteristic: organism
characteristics.text: Botrylloides leachii
characteristics.characteristic: scientific_name
characteristics.text: whole
characteristics.characteristic: tissue_type
characteristics.text: chowder_bay_botrylloides leachii
characteristics.characteristic: title