characteristics.text: ERC000024
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-18T00:19:24Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-18T00:19:24Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112157181
characteristics.characteristic: External Id
characteristics.text: Marine College, Shandong University
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-18T00:19:24Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-18T00:19:24Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14267027
characteristics.characteristic: SRA accession
characteristics.text: 3-1899-Z.fasta
characteristics.characteristic: Submitter Id
characteristics.text: marine biome [ENVO:00000447]
characteristics.characteristic: broad-scale environmental context
characteristics.text: 2019-05-01
characteristics.characteristic: collection date
characteristics.text: 0.5
characteristics.unit: m
characteristics.characteristic: depth
characteristics.text: Marine macroalgae, surrounding seawater, and surrounding sediment were collected at Weihai, China (122.12 N, 37.56 E). Phycosphere bacteria: Macroalgae were cut into pieces and rinsed thrice with sterile seawater. Afterwards, 10 g of these pieces were washed with 10 ml sterile seawater (rotary shaker, 170 rpm., 30 min, 25 °C). One-milliliter aliquots were diluted stepwise to 1:100,000 (sterile seawater), and 100 μl were subsequently plated and incubated (21 d, 28 °C). Seawater samples: One-milliliter seawater was diluted stepwise with 9 ml sterile seawater to 1:1,000. Aliquots of 100 μl were then plated and incubated as described above. Sediment samples: Sediment samples of 1 g were thoroughly mixed with 9 ml sterile seawater (rotary shaker, 170 rpm., 30 min, 25 °C) and then diluted in 1:10 steps with sterile seawater to 1:10,000. Afterwards, 100 μl aliquots were plated. Two media were used for plating, modified 2216E and modified VY/2 medium. Plating was completed within 2 h after sampling. After incubation, colony-forming units were counted with numbers ranging from 10 to 500. Colonies were selected depending on color, size, and shape. Picked colonies were purified by serial cultivation on plates with identical media. Purified strains were stored at -80 °C in a sterile 1% (w/v) saline medium with 15% (v/v) glycerol.
characteristics.characteristic: description
characteristics.text: coastal sea water
characteristics.characteristic: environmental medium
characteristics.text: China
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 37.562
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 122.121
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: isolate bacterial draft genome
characteristics.characteristic: investigation type
characteristics.text: microbial feature [ENVO:00002150]
characteristics.characteristic: local environmental context
characteristics.text: uncultured Marinobacter sp.
characteristics.characteristic: organism
characteristics.text: This study represents the draft genome sequences of bacterial isolates retrieved from the phycosphere of marine macroalgae, surrounding seawater, and surrounding sediments at Weihai, China.
characteristics.characteristic: project name
characteristics.text: uncultured Marinobacter sp.
characteristics.characteristic: scientific_name
characteristics.text: Illumina HiSeq-PE150
characteristics.characteristic: sequencing method
characteristics.text: 3-1899-Z.fasta
characteristics.characteristic: title
characteristics.text: marine seawater
characteristics.characteristic: water environmental package