characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-12-31T00:58:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-12-31T00:58:36Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111329176
characteristics.characteristic: External Id
characteristics.text: Philipps University Marburg, Bioinformatics Core Facility, Center for Tumor- and Immune Biology,
characteristics.characteristic: INSDC center name
characteristics.text: 2022-12-31T00:58:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-12-31T00:58:36Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13426740
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12141:YN021
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: CD8-Positive T-Lymphocytes
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were harvested and FACS sorted (FACSAria™ III (BD Biosciences)) for live cells before nucleic acid extraction CD8+ T cells were purified from human blood via buffy coat and purification of CD8+ positive cells using MACS and cultured (3-4 million) separately in plasma group(1ml) and ascites group(1ml) with activation beads ratio(cell : beads=2:1) for 16hr. Qiagen, RNeasy Plus Micro Kit Cat# 74034 Lexogen Quantseq
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: 8
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: YN021
characteristics.characteristic: title