characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-01T00:26:55Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-01T00:26:55Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10220355
characteristics.characteristic: External Id
characteristics.text: WASHINGTON UNIVERSITY SCHOOL OF MEDICINE
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-01T00:26:55Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-01T00:26:55Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7876711
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10995:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: 8 weeks
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: The mice were sacrificed and the fecal samples were collected. Mice were housed in an enhanced barrier specific pathogen-free facility under a strict 12-hour light cycle. Mice were maintained on a C57BL/6 background. All study protocols were approved by the Washington University Animal Studies Committee. Mice were fed irradiated standard diet (PicoLab Rodent Chow 20, Purina Mills) or western diet (Research Diets D09100301; with calories provided by 40% carbohydrates, 40% fat, and 20% protein). DNA was isolated using Qiagen QIAmp DNA Stool Kit, according to the kit protocol. Total DNA concentration and purity was obtained from an absorbance ratio at 260nm and 280nm. 14 PCR amplicons, representing all 9 16S variable regions, were constructed using the Fluidigm Access Array System. 5ng/ul of DNA were input into each reaction. The sample inlets consisted of 1X High Fidelity FastStart Reaction Buffer without MgCl2 (Roche), 4.5nM MgCl2 (Roche), 5% DMSO (Roche), 200uM PCR Grade Nucleotide Mix (Roche), 0.05 U/μL 5 U/μL FastStart High Fidelity Enzyme Blend (Roche), 1X Access Array Loading Reagent (Fluidigm), 1ul DNA, and water. The primers were added to the assay inlets at 200nM forward and reverse primers with 1X Access Array Loading Reagent. The samples were harvested after PCR amplification was performed on the BioMark HD system from Fluidigm. Each sample was harvested and indexed using unique 10 base pair sequences with 14 rounds of PCR to incorporate each index sequence. All samples were pooled into 48 sample libraries and cleaned using bead purification.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: standard diet
characteristics.characteristic: diet
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: group A
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: feces
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57/BL6
characteristics.characteristic: strain
characteristics.text: Sample 1
characteristics.characteristic: title