characteristics.text: ERC000047
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-17T12:18:54Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-17T12:18:54Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112223196
characteristics.characteristic: External Id
characteristics.text: EMG
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-17T12:18:54Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-17T12:18:54Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14333995
characteristics.characteristic: SRA accession
characteristics.text: SRR19759413_bin.50_metawrap_v1.3_MAG
characteristics.characteristic: Submitter Id
characteristics.text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
characteristics.characteristic: assembly quality
characteristics.text: metaSPAdes v3.15.3
characteristics.characteristic: assembly software
characteristics.text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.
characteristics.characteristic: binning parameters
characteristics.text: metawrap v1.3
characteristics.characteristic: binning software
characteristics.text: mouse digestive system
characteristics.characteristic: broad-scale environmental context
characteristics.text: EBI-EMG
characteristics.characteristic: broker name
characteristics.text: not provided
characteristics.characteristic: collection date
characteristics.text: 99.37
characteristics.unit: %
characteristics.characteristic: completeness score
characteristics.text: CheckM
characteristics.characteristic: completeness software
characteristics.text: 0.63
characteristics.unit: %
characteristics.characteristic: contamination score
characteristics.text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR19759413 of study SRP382781.
characteristics.characteristic: description
characteristics.text: feces
characteristics.characteristic: environmental medium
characteristics.text: not provided
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not provided
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: not provided
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: metagenome-assembled genome
characteristics.characteristic: investigation type
characteristics.text: mouse gut metagenome
characteristics.characteristic: isolation_source
characteristics.text: digestive tube
characteristics.characteristic: local environmental context
characteristics.text: mouse gut metagenome
characteristics.characteristic: metagenomic source
characteristics.text: uncultured Lachnoclostridium sp.
characteristics.characteristic: organism
characteristics.text: A growing body of evidence supports the notion that the gut microbiome plays an important role in cancer immunity. However, the underpinning mechanisms remain to be fully elucidated. One attractive hypothesis envisages that among the T cells elicited by the plethora of microbiome proteins a few exist that incidentally recognize neo-epitopes arising from cancer mutations (molecular mimicry (MM) hypothesis). To support MM, the human probiotic Escherichia coli Nissle was engineered with the SIINFEKL epitope (OVA-E.coli Nissle) and orally administered to C57BL6 mice. The treatment with OVA-E.coli Nissle, but not with wild type E. coli Nissle, induced OVA-specific CD8+ T cells and inhibited the growth of tumors in mice challenged with B16F10 melanoma cells expressing OVA. The microbiome shotgun sequencing and the sequencing of TCRs from T cells recovered from both lamina propria and tumors provide evidence that the main mechanism of tumor inhibition is mediated by the elicitation at the intestinal site of cross-reacting T cells, which subsequently reach the tumor environment. Importantly, the administration of Outer Membrane Vesicles (OMVs) from engineered E. coli Nissle, as well as from E. coli BL21(DE3)DompA, carrying cancer-specific T cell epitopes also elicited epitope-specific T cells in the intestine and inhibited tumor growth. Overall, our data strengthen the important role of MM in tumor immunity and assign a novel function of OMVs in host-pathogen interaction. Moreover, our results pave the way to the exploitation of probiotics and OMVs engineered with tumor specific-antigens as personalized mucosal cancer vaccines.
characteristics.characteristic: project name
characteristics.text: SAMN29213147
characteristics.characteristic: sample derived from
characteristics.text: uncultured Lachnoclostridium sp.
characteristics.characteristic: scientific_name
characteristics.text: Illumina NovaSeq 6000
characteristics.characteristic: sequencing method
characteristics.text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Lachnoclostridium;s__
characteristics.characteristic: taxonomic classification
characteristics.text: multi-marker approach
characteristics.characteristic: taxonomic identity marker
characteristics.text: Metagenome-assembled genome: SRR19759413_bin.50_metawrap_v1.3_MAG
characteristics.characteristic: title