characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-01T00:28:22Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-01T00:28:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110452525
characteristics.characteristic: External Id
characteristics.text: Sun Yat-sun University Zhongshan Ophathlamic Center
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-01T00:28:22Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-01T00:28:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12550555
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-102251659426996:FMHippo_0801_H1-1
characteristics.characteristic: Submitter Id
characteristics.text: 13
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: crab-eating macaque
characteristics.characteristic: common name
characteristics.text: Protocols: Total 8 monkeys (4-15 years old, 8 males and 2 females, Macaca fascicularis) were used in the current project. All procedures complied with the NIH Guide for the Care and Use of Laboratory Animals approved by Sun Yat-sen University. Following 2 weeks of adaptive housing in the institutional animal facility, animals were anesthetized with a lethal dose of sodium pentobarbital. Animals were transcardially perfused with 2 liters of artificial cerebrospinal fluid (ACSF, in mM: 125 NaCl, 25 NaHCO3, 1 NaH2PO4, 2 KCl, 25 D-Glucose, 2 CaCl2, 1 MgCl2, 5 sodium l-ascorbate, 3 sodium pyruvate, 0.01 taurine, 2 thiourea, 1 kynurenic acid, 0.1 DL-AP5, and 1x10-3 tetrodotoxin). The flow of perfusion fluid was adjusted so that the perfusion finished within approximately 40 minutes. The brain was removed from the skull immediately and a block of tissue containing the hippocampus was cut off. The position of hippocampus referred to the atlas of the rhesus monkey brain (Saleem and Logothetis, 2012). The tissue block was kept in the ACSF and sectioned on a vibratome (VT1200S, Leica) in 300 um thick slices. The adherent tissues around the hippocampus of each slice were removed on ice and only the regions of interest were kept for dissociation. 10X single cell isolation protocol v2 10X Genomics Chromium platform v2
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: FM_0801
characteristics.characteristic: individual
characteristics.text: Macaca fascicularis
characteristics.characteristic: organism
characteristics.text: Macaca fascicularis
characteristics.characteristic: organism
characteristics.text: brain
characteristics.characteristic: organism part
characteristics.text: hippocampus
characteristics.characteristic: sampling site
characteristics.text: Macaca fascicularis
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: FMHippo_0801_H1-1
characteristics.characteristic: title