characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-20T20:30:34Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-20T20:30:34Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111452416
characteristics.characteristic: External Id
characteristics.text: Department of Biochemistry University of Cambridge, UK
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-20T20:30:34Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-20T20:30:34Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: rRNA_depleted_total_RNA
characteristics.characteristic: RNA source
characteristics.text: ERS13545814
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12264:untreated.rep2.rna
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Cells were collected by filtration. Filters were immediately frozen in liquid nitrogen. Cells were grown at 32C in EMM2 (Moreno et al. Methods Enzymology 194: 795). Total RNA was extracted using a hot phenol extraction method as described in Lyne et al. (2003) BMC Genomics 4:27, with an initial 45min incubation at 65 C. RNAs DNase treated, purified by Purelink micro columns and ribo-depleted with NEB rRNA depletion module (E7870L) with Yeast Probes 30 ng of ribo-depleted RNA was used as starting material for the NEXTflex Rapid Directional qRNA-Seq Kit V2 (Bioo Scientific).
characteristics.characteristic: description
characteristics.text: Schizosaccharomyces pombe 972h-
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe 972h-
characteristics.characteristic: organism
characteristics.text: 2
characteristics.characteristic: replicate
characteristics.text: Schizosaccharomyces pombe 972h-
characteristics.characteristic: scientific_name
characteristics.text: untreated.rep2.rna
characteristics.characteristic: title