characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-01T00:29:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-01T00:29:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112151160
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF CAMBRIDGE
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-01T00:29:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-01T00:29:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14261060
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12339:SNCA3x_UNT
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: EDi001-B
characteristics.characteristic: cell_line
characteristics.text: dopaminergic neuron
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: The differentiation protocol of midbrain organoids was adapted. Briefly, we patterned the cells using the Kriks et al. 2011 protocol http://dx.doi.org/10.1038/nature10648 and we matured the neurons using the Jo et al. protocol . Dissociated single cells were washed with PBS three times, and counted and checkedfor viability on a Countess 3FL. 10,000 live single cells per sample were loaded onto the Chromium controller (10X Genomics) for single cell partitioning and barcoding. midbrain organoid dopaminergic differentiation Chromium Next GEM Single Cell 3' Kit v3.1 Chromium Next GEM Single Cell 3' Kit v3.1
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Parkinson's disease
characteristics.characteristic: disease
characteristics.text: SNCA-3x overexpression
characteristics.characteristic: genotype
characteristics.text: monoculture
characteristics.characteristic: growth condition
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: floor plate of midbrain
characteristics.characteristic: organism part
characteristics.text: induced pluripotent stem cell
characteristics.characteristic: progenitor cell type
characteristics.text: skin
characteristics.characteristic: progenitor organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: SNCA3x_UNT
characteristics.characteristic: title