characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-01T00:28:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-01T00:28:36Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111329122
characteristics.characteristic: External Id
characteristics.text: National Taiwan University Hospital
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-01T00:28:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-01T00:28:36Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13426687
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12144:Sotorasib-resistant MIAPaCa2 cells
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: MIA Paca-2
characteristics.characteristic: cell_line
characteristics.text: pancreatic ductal cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: LU65 cells and MiaPaCa2 cells were grown in increasing dose of Sotorasib for more than 1 month to establish sotorasib-resistant cells. Cell pellets of parental and sotorasib-resistant cells were collected. Sotorasib-resistant cells were grown in culture media with 5 micromole sotorasib Genomic DNA was extracted using DNA purification kits from Qiagen 100ng of DNA was used for DNA library preparation with Illumina DNA Prep with Enrichment kit
characteristics.characteristic: description
characteristics.text: pancreatic carcinoma
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Sotorasib resistant
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sotorasib-resistant MIAPaCa2 cells
characteristics.characteristic: title