characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-11-02T16:00:07Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-02T00:21:45Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10157790
characteristics.characteristic: External Id
characteristics.text: Laboratory of Genomics and Bioinformatics Institute of Molecular Genetics of the Czech Academy of Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2022-11-02T16:00:07Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-02T00:21:45Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7814277
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10956:E
characteristics.characteristic: Submitter Id
characteristics.text: 28
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: HSCs were sorted using an Influx instrument (BD Biosciences, San Jose, CA, USA), and lysed with Tri Reagent RT (Molecular Research Center, Cincinnati, OH, USA. Mice were sacrificed by cervical dislocation, femurs and tibias were isolated, and crunched using pestle and mortar. After obtaining single-cell suspensions, red blood cells were lysed. WT HSCs were exposed in vivo to WT or CMO BM niche. RNA was isolated using RNEasy MinElute Cleanup Kit (Qiagen, Venlo, Netherlands). Libraries (300 pg per sample) were prepared with SMARTer® Stranded Total RNA-Seq - Pico Input Mammalian library preparation kit v2 (Takara).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: transplanted into wild type mouse bone marrow niche
characteristics.characteristic: growth condition
characteristics.text: E1;E2;E3
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: hematopoietic stem cell
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: E
characteristics.characteristic: title