characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-12-31T00:57:26Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-12-31T00:57:26Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11046380
characteristics.characteristic: External Id
characteristics.text: VICTOR CHANG CARDIAC RESEARCH INSTITUTE
characteristics.characteristic: INSDC center name
characteristics.text: 2022-12-31T00:57:26Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-12-31T00:57:26Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8696097
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11161:QSi5
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Liver specimens were obtained from QSi5 and 129T2/SvEms mice. Genomic DNA was extracted using the phenol-chloroform extraction method. 200ng of DNA was mechanically fragmented using LE220 Covaris (Covaris, Woburn, USA) to approximately 450 base pair inserts followed by library preparation using the Seqlab TruSeq Nano DNA HT kit (20000903, Illumina, San Diego, USA). Library preparation was performed according to manufacturer’s instructions which included end repair, library size selection, 3’ end polyadenylation, adaptor ligation, purification of ligated fragments, enrichment of ligated fragments by PCR and a final purification of amplified DNA library. Library fragment sizes were reviewed using the LabChip GX (Perkin Elmer, Waltham, USA) to evaluate library size and adapter dimer presence before the library concentration was determined via quantitative real time PCR using the KAPA library quantification kits (KK4824, Roche, Basel, Switzerland) on the QuantStudio7 or ViiA7 (Thermo Fisher Scientific, Waltham, USA). All libraries were normalised before sequencing.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: QSi5
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: not available
characteristics.characteristic: sex
characteristics.text: QSi5
characteristics.characteristic: strain
characteristics.text: QSi5
characteristics.characteristic: title