characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-04T00:19:38Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-02-04T00:19:38Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111348583
characteristics.characteristic: External Id
characteristics.text: LabMedMolGe
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-04T00:19:38Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-04T00:19:38Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13446130
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12167:Sample 4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: As described above, C. albicans cultures were grown with or without 10 mM NAM in Petri dishes of 90 mm diameter and incubated at 25°C. Three independent biological replicates were performed for either CTRL or CaNAM cells. After 28 h of incubation, a total of 109 yeast cells were harvested (8,000g, 10 min at 4°C) and washed with diethylpyrocarbonate (DEPC)-treated water. RNA isolation was performed as described by Vennapusa et al., 2020 with some modifications. Briefly, yeast cells were resuspended in 600 mL of RNA extraction buffer (100 mM Tris- HCl (pH: 8), 25 mM EDTA, PVP 2.5%, NaCl 2.5 M, 2.5 % β-Mercaptoethanol, 2% SDS) and disrupted mechanically with BeadBug microtube homogenizer (Benchmark Scientific, Sigma-Aldrich, Milan, Italy) by using acid-washed glass beads. Total RNA was isolated using an equal volume of Acid-Phenol/Chloroform, pH 4.5 with IAA, 125:24:1 (Sigma-Aldrich, Milan, Italy). Following centrifugation, the RNA was precipitated from the aqueous phase with 100% ethanol, washed twice with 75% ethanol, and dissolved in DEPC-water. RNA quantification was carried out with the instrument Nanodrop 200 Thermo Fisher Scientific (Monza, Italy), and its integrity was checked by electrophoresis on 1.5% agarose gel. For RNA sequencing, RNA quality was assessed with TapeStation (Agilent, Cernusco sul Naviglio, Italy), and only RNA with RIN > 8 was used for library production.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Candida albicans
characteristics.characteristic: organism
characteristics.text: Candida albicans
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Candida albicans
characteristics.characteristic: scientific_name
characteristics.text: Sample 4
characteristics.characteristic: title