characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-01T00:27:04Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-01T00:27:04Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10773773
characteristics.characteristic: External Id
characteristics.text: NORWEGIAN UNIVERSITY OF LIFE SCIENCES
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-01T00:27:04Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-01T00:27:04Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8425026
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11120:19-Ara48hGS4-0
characteristics.characteristic: Submitter Id
characteristics.text: 6
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: Shoots of the seedlings were harvested in liquid nitrogen after 48 h gamma exposure and stored at -80°C until analyses. Seeds of the Landsberg erecta (Ler) accession of Arabidopsis thaliana (L) Heyhn, were surface sterilized for 3 min with a solution of 10 mL of 70% ethanol and 10 ul Tween 20, followed by five times wash in distilled water. Seeds were given a quick rinse in 96% ethanol before air drying on a sterile filter paper inside a sterile hood. All seeds were evenly sown on 1/2 MS medium (Murashige & Skoog 1962; Duchefa Biochemie, Harleem, Netherland) with 0.8% agar (Plant agar, Sigma-Aldrich) in petri dishes of 5 cm diameter. The seeds were then germinated for 6 days at 20°C under a photon flux density of 30 μmol m-2 s-1 at 400-700 nm (TL-D 58W/840 lamps, Phillips) in a 16 h photoperiod. Six days old seedlings were exposed to gamma radiation for 48h at gamma dose rates 1, 10, 40, 100, or 290 mGy/h, using the FIGARO low dose gamma irradiation facility (60Co; 1173.2 and 1332.5 keV γ-rays) at Norwegian University of Life Sciences (Hansen et al. 2019). During the gamma irradiation, the room temperature was set at 20˚C±1°C with a 12 h photoperiod with a photon flux density of 55 μmol m-2 s-1 provided by high pressure metal halide lamps (HPI-T Plus 250W lamps, Phillips). Control samples not exposed to gamma radiation were positioned outside the radiation sector, shielded by lead walls, in the same room and under same light and temperature conditions. Total RNA was extracted using the Masterpure Plant RNA Purification Kit (Epicenter, Madison, WI, USA) following the manufacturer`s instructions except that 0.5 % polyvinylpyrrolidone (PVP), mw 360 000, Sigma Aldrich, Steinheim, Germany) was added to the extraction buffer and that 3 µl beta-mercaptoethanol per sample replaced the 1,4-dithiothreitol (DTT) in the manufacturer`s protocol. RNA quality was assessed using an Agilent 2100 Bioanalyzer with an RNA 144000 NanoKit (Agilent technologies, Palo Alto, CA, USA) and the quantity of isolated RNA was measured by a NanoDrop ND-1000 Spectrophotometer (Thermo Scientific, Wilmington, DE, USA). Strand-specific 20xTruseqTM RNA library preparation kit.
characteristics.characteristic: description
characteristics.text: seedling development stage
characteristics.characteristic: developmental stage
characteristics.text: Landsberg erecta
characteristics.characteristic: ecotype
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: shoot
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: 19-Ara48hGS4-0
characteristics.characteristic: title