characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-01T00:28:16Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-01T00:28:16Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110321882
characteristics.characteristic: External Id
characteristics.text: Garvan Institute of Medical Research
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-01T00:28:16Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-01T00:28:16Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12419490
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11936:Sample 17
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Mel-224
characteristics.characteristic: cell line
characteristics.text: epithelial cell
characteristics.characteristic: cell type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Human melanoma cell lines CHL-1 (WT), MEL224 (NRAS Q61R ) and A375 (BRAF V600E ) were transfected with expression vectors for GLI1, GLI2 or GLI3 proteins in two independent experiments. Non-transfected lines were used as controls. Briefly, 200 000 cells were seeded in a Ø10 cm cell culture dish and transfected the next day with 5 g of plasmid DNA, using the X-fect reagent (Clontech) and following the manufacturer’s instructions. 48 hours post transfection, total RNA was isolated with Agilent Absolutely RNA miRNA Kit. The quality of the isolated RNA was checked on agarose gel, and concentrations of RNA were measured on NanoPhotometer N60 instrument (Implen, Munich, Germany). Sequencing libraries were generated and sequenced by DNA Link Company. The integrity of RNA samples was checked on Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The instrument provided data on RNA concentration, electropherogram, rRNA subunit ratios and RNA integrity number (RIN). All samples had RIN ≥ 8.0 and a 28S:18S ratio ≥ 1.4. Total amount of 1 ug of RNA was used. cDNA libraries were prepared using TruSeq mRNA library Kit.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: melanoma
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: skin
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 17
characteristics.characteristic: title