characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-07T13:25:38Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-02-07T13:25:38Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111374555
characteristics.characteristic: External Id
characteristics.text: Hematology Research Unit, Groupe Interdisciplinaire de Genoproteomique Appliquee (GIGA)-I3, University of Liege, Liege, Belgium
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-07T13:25:38Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-07T13:25:38Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13471546
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12188:human T cells extracted from spleen of PTCy-treated mice
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: T cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: NSG mice were engrafted with human PBMC on day 0 to induce xenogeneic GVHD and treated (n=4) or not (n=3)with cyclophosphamide 100 mg/kg on day +3. Mice were sacrificed on day +6 and human CD3+ T cells were isolated from the spleen by immunomagnetic selection and pooled for each group. NSG mice were engrafted on day 0 with human PBMC and sacrificed on day 6 to harverst spleen. Human CD3+ T cells were isolated from mice spleen by immunomagnetic selection. Treated mice received a single Cyclosphophamide (100mg/kg) i.p. injection on day 3 post-transplantation Isolated cells were washed once with PBS + 0.04% BSA. Cell concentration was adjusted to 1,000 total cells/µl and 13,300 cells were loaded on Chromium Controller (10X Genomics). Samples were processed for droplet-based-RNA sequencing and libraries were prepared using Chromium Single Cell 3' Reagent Kits V3 according to manufacturer's protocol (10x Genomics). Amplified cDNA quality controls were performed with an Agilent bioanalyzer and final library profile were checked on Qiaxcel (Qiagen).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: graft versus host disease
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: human T cells extracted from spleen of PTCy-treated mice
characteristics.characteristic: title
characteristics.text: transplanted in vivo into NSG mice for 6 days
characteristics.characteristic: xenograft