characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-12-31T00:57:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-12-31T00:57:08Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10160864
characteristics.characteristic: External Id
characteristics.text: University of Namur
characteristics.characteristic: INSDC center name
characteristics.text: 2022-12-31T00:57:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-12-31T00:57:08Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7817347
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10969:KC27_CTL5J
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: keratinocyte
characteristics.characteristic: cell type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Normal human epidermal keratinocytes (NHEKs) were isolated from foreskin sample of young donors (less than 7 years) as described (Gilchrest, 1983, doi: 10.1111/1523-1747.ep12541084). Samples were obtained after circumcision following approval by the ethic committee of the Clinique St Luc (Bouge, Belgium). NHEKs were grown in specific keratinocyte serum-free medium (K-SFM) (Invitrogen, 15005042), supplemented with bovine pituitary extract (50 µg/ml) and human recombinant epidermal growth factor (5 ng/ml). For UVB-SIPS model, early passage NHEKs were seeded at low confluence in K-SFM. At 72 h after plating, cells were exposed to UVB radiation through a thin layer of PBS. UVB (NB-UVB) used are generated by a Narrow-Band UVB lamp (Philips, TL20W/01 lamps) with a peak at 311 nm. The emitted radiation was checked under the flask lid using a UVB radiometer with a UVB sensor (Bioblock Scientific). After irradiation, PBS was replaced by K-SFM medium. UVB exposures are repeated three times on a single day with a three-hours interval. Unexposed cells (controls) were kept in the same culture conditions without UVB exposure. Total RNA was extracted using RNeasy mini kit (Qiagen) according to manufacturer’s instructions. RNA integrity was checked with Agilent Bioanalyzer. Sequencing mRNA libraries were prepared using TruSeq® Stranded mRNA library preparation kit (Illumina) with 1 µg of total RNA by condition.
characteristics.characteristic: description
characteristics.text: juvenile stage
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: replicating cells
characteristics.characteristic: environmental history
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: 2
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: skin of prepuce of penis
characteristics.characteristic: organism part
characteristics.text: 4 to 5
characteristics.characteristic: passage
characteristics.text: 5
characteristics.unit: day
characteristics.characteristic: sampling time point
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: KC27_CTL5J
characteristics.characteristic: title