characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-01T00:28:28Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-01T00:28:28Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110664770
characteristics.characteristic: External Id
characteristics.text: School of Medicine, Cardiff University, UK
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-01T00:28:28Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-01T00:28:28Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12762350
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12090:Cdk12 Mutant 3
characteristics.characteristic: Submitter Id
characteristics.text: 7
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: Whole Drosophila heads (N=6-7 heads pooled per sample) from 7-day old females, of the desired genotypes, were dissected and snap frozen for later use. RNA extraction was performed according to the RNAqueous-Micro kit (AM1931). Quality control of RNA was performed using Qubit and tapestation. Next, samples were normalised and library prep was performed using an Illumina TruSeq stranded mRNA kit. This kit involved poly-A enrichment and TruSeq3 adapter ligation.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Sp/CyO;Cdk12* FRT2A, FRT82B/TM3
characteristics.characteristic: genotype
characteristics.text: mixed pool of 6 to 7 heads
characteristics.characteristic: individual
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: head
characteristics.characteristic: organism part
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: Cdk12 Mutant 3
characteristics.characteristic: title