characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-01T00:28:27Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-01T00:28:27Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110663776
characteristics.characteristic: External Id
characteristics.text: Technion, Israel
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-01T00:28:27Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-01T00:28:27Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12761352
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12096:76_1
characteristics.characteristic: Submitter Id
characteristics.text: 8
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: neural cell
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Transcardial aCSF perfusion, brain extraction and sectioning, microdissection of full amygdala, single-cell dissociation, scRNA-seq; as described in Zeisel et al. 2018 7-11 week old male C57Bl/6JOlaHsd mice were habituated to a behavioral arena for 20min, followed by 6 cycles of (foot shock-tone shock (US-CS) pairing: 35sec tone (CS), 750ms 0.7mV foot shock (US), 24sec rest. For learning and consolidation, mice were sampled 2h, 8h and 24h after tone-cued fear condition (CFC). For recall, 24h after CFC, 4 mice were exposed to 6 cycles of the tone cue alone (35sec CS, 25sec rest). The 3 mice exhibiting strongest freezing behavior compared to non-FC controls undergoing the same session were sacrificed 2h later. Naïve, home cage (HC) littermates served as controls in each experimental batch. 10Xgenomics 3' V3 10Xgenomics 3' V3
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: amygdala
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6JOlaHsd
characteristics.characteristic: strain
characteristics.text: 76_1
characteristics.characteristic: title