characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-12-31T00:58:39Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-12-31T00:58:39Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111479566
characteristics.characteristic: External Id
characteristics.text: Institute for Integrative Biology of the Cell (I2BC)
characteristics.characteristic: INSDC center name
characteristics.text: 2022-12-31T00:58:39Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-12-31T00:58:39Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13573601
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-117041666018888:F Exconjugant #1 corrected
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: ~210 000 colonies were scraped from the LB agar plate containing appropriate antibiotics total gDNA was purified by GenElute Bacterial Genomic DNA kit (Sigma-Aldrich, St. Louis, MO) Library preparation was carried out as described by Espinosa et al. (https://doi.org/10.1016/j.xpro.2020.100202). In brief, DNA was first digested with MmeI. Fragment containing Tn was size-fractioned and then adaptor-ligated. PCR was carried out to add appropriate adapters while amplify the target. Size-fractioned and purified PCR product was subjected to sequencing.
characteristics.characteristic: description
characteristics.text: MC1061::Tn / F
characteristics.characteristic: genotype
characteristics.text: YAM32
characteristics.characteristic: individual
characteristics.text: Escherichia coli K-12
characteristics.characteristic: organism
characteristics.text: Escherichia coli K-12
characteristics.characteristic: organism
characteristics.text: Escherichia coli K-12
characteristics.characteristic: scientific_name
characteristics.text: F Exconjugant #1 corrected
characteristics.characteristic: title