characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-12-31T00:58:39Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2022-12-31T00:58:39Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111451415
characteristics.characteristic: External Id
characteristics.text: INSERM Gustave Roussy Cancer Center
characteristics.characteristic: INSDC center name
characteristics.text: 2022-12-31T00:58:39Z
characteristics.characteristic: INSDC first public
characteristics.text: 2022-12-31T00:58:39Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13544816
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12255:6_EG
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HEL9217
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: RNA were isolated from 4 different HEL cell lines: GFP (CTRL), ETO2-GLIS2, ETO2-GLIS2 C265G and ETO2-GLIS2 dNHR2 following 24h of induction with 500 ng/mL of doxycycline (DOX). RNA were isolated using the RNeasy Mini/Micro Kit (Qiagen) and quantified by NanoDrop (ThermoScientific) cDNA libraries were synthesized from 250 ng total RNA using TruSeq Strandard mRNA Kit (Illumina). Libraries were verified for their amount and quality by capillary electrophoresis using a 2100 Bioanalyzer (Agilent Technologies).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: leukemia
characteristics.characteristic: disease
characteristics.text: ETO2-GLIS2 expression
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: bone marrow
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: doxycycline
characteristics.characteristic: stimulus
characteristics.text: 6_EG
characteristics.characteristic: title