characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-10-13T09:00:04Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-31T00:19:50Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111264481
characteristics.characteristic: External Id
characteristics.text: Departamento de Produccion Animal, Facultad de Veterinaria, Universidad de Leon
characteristics.characteristic: INSDC center name
characteristics.text: 2022-10-13T09:00:04Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-31T00:19:50Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13362100
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12130:Male 5
characteristics.characteristic: Submitter Id
characteristics.text: 28
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: Assaf
characteristics.characteristic: breed
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: sheep
characteristics.characteristic: common name
characteristics.text: Protocols: Perirenal fat samples were collected from 12 Assaf suckling lambs (6 males and 6 females). All animals were born at the Instituto de Ganadería de Montaña (IGM) (Grulleros, León, Castilla y León, Spain) in the same lambing season (winter 2019-2020) from primiparous Assaf dairy ewes. The lambs were reared under the same management and diet conditions. Initially, during the first 24 hours after lambing, the lambs were kept with their dams to suck the colostrum. Subsequently, the animals were held in lamb pens fed with artificial milk using Ovilac 60 (Calfvet®) milk replacer powder ad libitum up to slaughter to remove the influence of maternal effects. The lambs were slaughtered at a local slaughterhouse at the market weight for the PGI label “lechazo de Castilla y Leon” (9-12 kg), which has average ages of 28.16 (±4.87) and 24.16 (±5.19) for males and females, respectively. The management, transport and sacrifice of the animals were in accordance with Spanish and EU legislation [Spanish Laws 32/2007, 6/2013 and RD 37/2014; Council Regulation (EC) 199/2009]. For RNA extraction, the sampled tissues were preserved in an RNA-stabilization solution (Ambion RNAlater; Life Technologies) and stored at 4 °C for 24 h. Subsequently, the RNA-stabilization solution was removed, and the samples were frozen at -80 °C until RNA extraction. RNA was extracted using the miRNeasy Mini KIT (Qiagen, Germany) with adaptations for use in adipose tissue. For RNA extraction, the sampled tissues were preserved in an RNA-stabilization solution (Ambion RNAlater; Life Technologies) and stored at 4 °C for 24 h. Subsequently, the RNA-stabilization solution was removed, and the samples were frozen at -80 °C until RNA extraction. RNA was extracted using the miRNeasy Mini KIT (Qiagen, Germany) with adaptations for use in adipose tissue.
characteristics.characteristic: description
characteristics.text: infant
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: GP26
characteristics.characteristic: individual
characteristics.text: Ovis aries
characteristics.characteristic: organism
characteristics.text: Ovis aries
characteristics.characteristic: organism
characteristics.text: perirenal fat
characteristics.characteristic: organism part
characteristics.text: Ovis aries
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: Male 5
characteristics.characteristic: title