characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-01T00:28:23Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-01T00:28:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110586279
characteristics.characteristic: External Id
characteristics.text: The University of Texas MD Anderson Cancer Center Department of Lymphoma & Myeloma
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-01T00:28:23Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-01T00:28:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12684061
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12046:HC6
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: T cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Tumor and T cells from splenic lymphocytes from ten HCLc patients were purified using FACS. Splenocytes were stained with CD3-PE (clone: UCHT1, Biolegend), 20 μg/ml CD11c-FITC (clone: Bu15, Biolegend), 5 μg/ml CD103-PE/Cy7 (clone: Ber-ACT8, Biolegend), and 2.5 μg/ml CD19-APC (clone: HIB19, Biolegend). Tumor cells were isolated based on the co-expression of CD19, CD11c and CD103; and T cells based on CD3 expression. Data was analyzed using FACS Diva (BD) and FlowJo (TreeStar). Genomic DNA from purified cell populations were extracted using QIAamp DNA mini kit (Qiagen, Manchester, UK), according to the manufacturer’s instructions. DNA from tumor and T cells were used for library preparation using NEBNext UltraTM DNA Library Prep kit (New England Biolabs, Hitchin, UK), according to manufacturer’s instructions.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: hairy cell leukemia
characteristics.characteristic: disease
characteristics.text: HCL6
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: spleen
characteristics.characteristic: organism part
characteristics.text: neoplasm
characteristics.characteristic: sampling site
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: HC6
characteristics.characteristic: title