characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-31T00:19:55Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-31T00:19:55Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111476243
characteristics.characteristic: External Id
characteristics.text: UoH
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-31T00:19:55Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-31T00:19:55Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13570279
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12297:IgG2a.1
characteristics.characteristic: Submitter Id
characteristics.text: 5 to 6
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: golden hamster
characteristics.characteristic: common name
characteristics.text: Protocols: HapT1 cell line described in this publication DOI: 10.18632/oncotarget.9729 Thin needle biospies were taken from PDAC tumours on the day before the last injection. Biopsies were placed in RNA-later for extraction. 5-6 week old Syrian hamsters were engrafted with HapT1 cells grown in culture. The cells were grown (3x passages) in RPMI (10%FBS, 1%L-Glut, 1% P/s) until 80% confluency and were washed three times before engraftment. Hamsters were intraperitoneally injected with either 300ug of IgG2a or anti-PD-L1 every three days for a total of 8 injections. After extraction of gene hit counts, the gene hit counts table was used for downstream differential expression analysis. Using DESeq2, a comparison of gene expression between the customer-defined groups of samples was performed. The Wald test was used to generate p-values and log2 fold changes. Genes with an adjusted p-value < 0.05 and absolute log2 fold change > 1 were called as differentially expressed genes for each comparison. Below are the results of the number of significantly differentially expressed genes for all comparisons provided. RNAeasy mini kit (qiagen) was used according to the manufacturers instructions PolyA selection
characteristics.characteristic: description
characteristics.text: pancreatic ductal adenocarcinoma
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Mesocricetus auratus
characteristics.characteristic: organism
characteristics.text: Mesocricetus auratus
characteristics.characteristic: organism
characteristics.text: pancreas
characteristics.characteristic: organism part
characteristics.text: neoplasm
characteristics.characteristic: sampling site
characteristics.text: Mesocricetus auratus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: HsdHan®:AURA
characteristics.characteristic: strain
characteristics.text: IgG2a.1
characteristics.characteristic: title
characteristics.text: 5 x10E+6 HapT1 cells into left flank until neoplasm formation
characteristics.characteristic: xenograft