characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-01T00:29:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-01T00:29:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112151157
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF CAMBRIDGE
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-01T00:29:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-01T00:29:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14261057
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12339:KOLF2_ROT
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: WTSIi018-B-1
characteristics.characteristic: cell_line
characteristics.text: dopaminergic neuron
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: The differentiation protocol of midbrain organoids was adapted. Briefly, we patterned the cells using the Kriks et al. 2011 protocol http://dx.doi.org/10.1038/nature10648 and we matured the neurons using the Jo et al. protocol . Dissociated single cells were washed with PBS three times, and counted and checkedfor viability on a Countess 3FL. 10,000 live single cells per sample were loaded onto the Chromium controller (10X Genomics) for single cell partitioning and barcoding. midbrain organoid dopaminergic differentiation no antioxidants + 0.125 uM rotenone treatment for 24 hours Chromium Next GEM Single Cell 3' Kit v3.1 Chromium Next GEM Single Cell 3' Kit v3.1
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: monoculture
characteristics.characteristic: growth condition
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: floor plate of midbrain
characteristics.characteristic: organism part
characteristics.text: induced pluripotent stem cell
characteristics.characteristic: progenitor cell type
characteristics.text: skin
characteristics.characteristic: progenitor organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: KOLF2_ROT
characteristics.characteristic: title