characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-09T00:14:20Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-09T00:14:20Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111351393
characteristics.characteristic: External Id
characteristics.text: Nantes Universite, Inserm, The enteric nervous system in gut and brain disorders, IMAD, F-44000, France
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-09T00:14:20Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-09T00:14:20Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13448940
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12176:44_ED4_D
characteristics.characteristic: Submitter Id
characteristics.text: 8 to 10
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: ED
characteristics.characteristic: cage
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Distal colon fragments were collected then snap-frozen Distal colon samples were lysed in RA1 buffer (Macherey-Nagel) before total RNA extraction with the NucleoSpin RNA kit according to the manufacturer’s instructions (Macherey-Nagel). Purified mRNA were used for the sequencing The libraries was prepared from 10 ng of total RNA. 15 RNA samples were extracted from hiPS. The mRNA poly(A) tail was tagged with universal adapters, well-specific barcodes and unique molecular identifiers (UMIs) during template-switching reverse transcriptase. Barcoded cDNAs from multiple samples were then pooled, amplified and tagmented using a transposon-fragmentation approach, which enriches for 3’ends of cDNA. 100ng of full-length cDNAs was used as input to the Nextera DNA Sample Prep kit (ref FC-121-1030, Illumina), which enriches for 3′ends of cDNA. Size library is controlled on 2200 Tape Station Sytem (Agilent Technologies).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: control
characteristics.characteristic: environmental history
characteristics.text: serie2
characteristics.characteristic: experimental series
characteristics.text: Albino mouse, Tyrc/Tyrc, Tyrp1b/Tyrp1b, A/A - MHC: Haplotype H2d
characteristics.characteristic: genotype
characteristics.text: 44_ED4_D
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: descending colon
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: DSS
characteristics.characteristic: stimulus
characteristics.text: BALB/cJRj
characteristics.characteristic: strain
characteristics.text: 44_ED4_D
characteristics.characteristic: title