characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-01T00:28:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-01T00:28:36Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111328953
characteristics.characteristic: External Id
characteristics.text: Department of Molecular Plant Biology, Division for Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research (NIBIO)
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-01T00:28:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-01T00:28:36Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13426518
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12152:NCGR1603_wounded_inoculated_R2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: wild strawberry
characteristics.characteristic: common name
characteristics.text: NCGR1603
characteristics.characteristic: cultivar
characteristics.text: Protocols: All Fragaria vesca samples were collected from the crown (rhizome) of the plant, flash-frozen in liquid nitrogen and stored at -80 °C until RNA isolation. Sampling was performed at 48 hours after i) wounded and inoculated with Phytophthora cactorum, ii) wounded and inoculated with water, and iii) untreated control. Phytophthora cactorum was grown in V8 agar for two weeks at room temperature (20 °C) and zoospore suspension were made in autoclaved pond water. Zoospores were observed under a light microscope and counted using a hemocytometer-type grid KOVA® Glasstic Slide (Hycor, USA). Plants were gently wounded in the crown with a sterilised scalpel and inoculated with 2 mL of Phytophthora cactorum zoospore suspension (2 × 105 spores/mL) or water. Frozen crown tissues were ground using liquid nitrogen and total RNA was isolated using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, USA) according to the manufacturer’s instruction. RNA was dissolved in elution buffer provided with the kit and diluted in nuclease-free water. RNA quantity and quality was measured with the Nanodrop 2000 Spectrophotometer (Thermo Scientific, USA) and the Agilent RNA 6000 Nano kit with Agilent 2100 Bioanalyzer (Agilent Technology, USA). Libraries were prepared with the TruSeqTM stranded total RNA library prep kit (Illumina, USA).
characteristics.characteristic: description
characteristics.text: sporophyte vegetative stage
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: mixed pool of 4 plants
characteristics.characteristic: individual
characteristics.text: Phytophthora cactorum
characteristics.characteristic: infect
characteristics.text: Fragaria vesca
characteristics.characteristic: organism
characteristics.text: Fragaria vesca
characteristics.characteristic: organism
characteristics.text: rhizome
characteristics.characteristic: organism part
characteristics.text: Phytophthora cactorum resistant
characteristics.characteristic: phenotype
characteristics.text: Fragaria vesca
characteristics.characteristic: scientific_name
characteristics.text: NCGR1603_wounded_inoculated_R2
characteristics.characteristic: title