characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-01-01T00:28:50Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-01-01T00:28:50Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111415843
characteristics.characteristic: External Id
characteristics.text: OXFORD
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-01T00:28:50Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-01-01T00:28:50Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13509487
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12226:mLN6
characteristics.characteristic: Submitter Id
characteristics.text: 11 to 14
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: regulatory T cell
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mesenteric lymph nodes were collected in PBS/BSA/EDTA and passed through a 70 μm filter. Single cell suspensions were washed in PBS/BSA/EDTA and stained with fluorochrome-conjugated antibodies in PBS/BSA/EDTA for 20 min at 4 oC in the dark. Cells were washed and resuspended in PBS/BSA/EDTA/DNase1 for FACS sorting. Foxp3+ Tregs were sorted as live CD45+Lineage-CD4+TCRβ+Foxp3-YFP+ cells. Lineage included CD8, TCRγδ, B220, CD11b, CD11c, Gr1. Dead cells were excluded using DAPI. For gene expression analysis by RNA sequencing, small bulks of 300 Treg cells were sorted into 2μL lysis buffer (0.8% Triton X-100 and 2 U/μL RNase inhibitor) in a 96-well plate Sequencing libraries were prepared following the Smart-seq2 protocol (Picelli et al., 2013) with own in-house indexing primers (Lamble et al., 2013)
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: DGAT1-/-
characteristics.characteristic: genotype
characteristics.text: 6
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: mesenteric lymph node
characteristics.characteristic: organism part
characteristics.text: 6
characteristics.characteristic: replicate
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: mixed
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: mLN6
characteristics.characteristic: title