characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-17T00:25:51Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-17T00:25:51Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110005288
characteristics.characteristic: External Id
characteristics.text: Beatson Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-17T00:25:51Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-17T00:25:51Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12154355
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11793:Sample 8
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: PC-3
characteristics.characteristic: cell line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Subpopulations of a prostate cancer cell line (PC-3) where generated according to differential cortical levels of the transmembrane protein Podocalyxin. RNA was extracted using the RNeasy kit (Qiagen) including a DNase digestion step using RNase-Free DNase Set (Qiagen), as per manufacturers instructions, followed by reverse transcription by using High-Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific). Libraries were then prepared using manufacturers standard procedures (Illumina TruSeq stranded mRNA).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: prostate cancer
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: prostate gland
characteristics.characteristic: organism part
characteristics.text: low surface Podocalyxin
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific name
characteristics.text: Sample 8
characteristics.characteristic: title