characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-20T00:20:06Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-02-20T00:20:06Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112634839
characteristics.characteristic: External Id
characteristics.text: Laboratory of Molecular Medicine and Genomics
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-20T00:20:06Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-20T00:20:06Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14626819
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12558:Sample 4
characteristics.characteristic: Submitter Id
characteristics.text: 6
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: 10-week-old C57BL/6J mouse models of type 1 Diabetes mellitus were generated through a single high dose of streptozotocin (STZ, 200 mg/Kg). Mice were fed with a normal chow diet (NCD). C57BL/6J mouse models of type 2 Diabetes mellitus were generated through a 60 kcal% high-fat diet (HFD; D12492, Research Diets) for four weeks, starting at 6 weeks of age. During the fourth week, mice receive daily injections of low-dose STZ (40 mg/Kg) over four consecutive days. C57BL/6J Control mice were fed with a NCD. RNA was extracted from whole heart sections of CTRL, T1DM and T2DM mice starting from OCT embedded tissues Ten sections of 20µm of thickness were collected in 1.5 ml tubes and RNA extraction was performed using RNA Purification kit (Norgen) Libraries were generated using depleted RNA obtained from 1 µg total RNA by TruSeq Sample Preparation RNA Kit (Illumina, Inc., San Diego, CA, USA) according to the manufacturers protocol without further modifications
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: type 1 diabetes mellitus
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: T1DM_1
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: heart
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: Sample 4
characteristics.characteristic: title