characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-04-19T12:16:18Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-04-19T12:16:18Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112283404
characteristics.characteristic: External Id
characteristics.text: Bioinformatics Group and Institute for Biochemistry Leipzig University
characteristics.characteristic: INSDC center name
characteristics.text: 2023-04-19T12:16:18Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-04-19T12:16:18Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14394255
characteristics.characteristic: SRA accession
characteristics.text: 2
characteristics.characteristic: Submitter Id
characteristics.text: E. coli DH5α was cultivated in LB medium until reaching an OD600 of 0.5 and subsequently mixed with Lead-II-acetate to a final concentration of 75 mM for in vivo lead probing. After incubation for 7 minutes at 37 °C, reactions were stopped with ice-cold EDTA and total RNA was extracted using peqGOLD TriFast® (VWR) according to the manufacturers protocol, including an additional DNase I digestion. From every total RNA sample, two RNA-seq libraries were prepared, utilizing Arabidopsis thaliana RNL and E. coli RtcB to specifically capture 2ʹ,3ʹ-cP and 5ʹ-OH carrying fragments.
characteristics.characteristic: description
characteristics.text: Escherichia coli DH5[alpha]
characteristics.characteristic: organism
characteristics.text: Escherichia coli DH5[alpha]
characteristics.characteristic: scientific_name
characteristics.text: lead-treated replicate 2
characteristics.characteristic: title