characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-01T00:17:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-01T00:17:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112138747
characteristics.characteristic: External Id
characteristics.text: EMBL
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-01T00:17:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-01T00:17:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14248056
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12396:Sample_E10_H3_rep1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: epidermal cell
characteristics.characteristic: cell_type
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: Embryos were collected during a 1h window in apple juice agar plates. Then, the plates were incubated at 25 °C for 12 h leading to a population composed exclusively by stage 15 embryos. These embryos were then dechorionated and fixed with paraformaldehyde according to standard procedures (Sullivan, William, Michael Ashburner, and R. Scott Hawley’s Drosophila protocols. Cold Spring Harbor Laboratory Press, 2000.). Stage 15 embryos from a line containing svb_E10::GFP and svb_7::dsRed transgenes were cross-linked and dissociated. Then the E10::GFP and 7::dsRed nuclei were isolated by fluorescence activated cell sorter. Chromatin from 250,000 nuclei of each cell sub-populations was isolated and used for ChIP with anti-H3K4me1 (Merck, 07-436) and anti-H3 antibodies (abcam, ab1791) using the iDeal ChIP-seq kit from Diagenode, according to the manufacturer's instructions (https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns). Libraries were prepared using the Ovation Ultralow V2 DNA-Seq library preparation kit (NuGen, https://lifesciences.tecan.com/ultralow-dna-seq-library-prep-kit) according to the manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: embryonic stage 15
characteristics.characteristic: developmental stage
characteristics.text: transgenic
characteristics.characteristic: genetic modification
characteristics.text: svb_E10::GFP, svb_7::dsRed
characteristics.characteristic: genotype
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: active svb_E10 enhancer
characteristics.characteristic: phenotype
characteristics.text: 1
characteristics.characteristic: replicate
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: Sample_E10_H3_rep1
characteristics.characteristic: title