characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-23T00:28:37Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-02-23T00:28:37Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112639086
characteristics.characteristic: External Id
characteristics.text: Center for Medical Genetics, Gent, Belgium Cancer Research Institute Gent, Belgium
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-23T00:28:37Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-23T00:28:37Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14631064
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12588:IMR32
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: IMR-32
characteristics.characteristic: cell_line
characteristics.text: neuroblast (sensu Vertebrata)
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: 50,000 cells were harvested and spin down for 5 min at 800g. Cells were grown in RPMI1640 medium supplemented with 10% foetal bovine serum, 2 mM L-Glutamine and 100 IU/ml penicillin/streptavidin at 37°C in a 5% CO2 humid atmosphere, supplemented with 10% tetracyclin-free FBS to avoid leakage. ATAC-seq was performed as previously described with minor changes (Buenrostro et al. 2015). 50,000 cells were lysed and fragmented using digitonin and Tn5 transposase (Nextera DNA Library Prep Kit, cat#FC-121-1030)). Samples were purified using the DNA clean & concentrator kit from Zymo (cat#D4013). The transposased DNA fragments were amplified using the KAPA HiFi HotStart Readymix (cat#07 958 927 001) and purified using Agencourt AMPure XP beads (Beckmamn Coulter, cat#A63881). Library concentrations were measured with the Illumina Kapa Library quantification kit (Lightcycler 480 qPCR mix cat#KK4854)
characteristics.characteristic: description
characteristics.text: neuroblastoma
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: brain
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: IMR32
characteristics.characteristic: title