characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-26T00:18:23Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-02-26T00:18:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112651914
characteristics.characteristic: External Id
characteristics.text: German Center for Neurodegenerative Diseases (DZNE)
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-26T00:18:23Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-26T00:18:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14649623
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12731:rimod35
characteristics.characteristic: Submitter Id
characteristics.text: 70
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: batch1
characteristics.characteristic: batch
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: The brain samples and/or bio samples were obtained from The Netherlands Brain Bank, Netherlands Institute for Neuroscience, Amsterdam (open access: www.brainbank.nl). All Material has been collected from donors for or from whom a written informed consent for a brain autopsy and the use of the material and clinical information for research purposes had been obtained by the NBB. Additional samples were provided by the Queen Square Brain Bank of Neurological Disorders and MRC, King College London. We complied to all relevant regulations of the mentioned brain banks for the work with these samples. Total RNA for smRNA-seq was isolated from frozen tissue using the TRIzol reagent (ThermoFischer Scientific). After isopropanol precipitation and 80% ethanol rinsing RNA pellet was resuspended in RNAse free water and up to 10 micrograms of RNA was incubated with 2U of Ambion DNAse I (ThermoFischer) at 37°C for 20 minutes. DNA-free RNA samples were then further purified by phenol-chloroform-isoamyl-alchol extraction followed by ethanol precipitation. The small RNA-seq libraries were prepared in two different batches. They were prepared from frozen tissue starting from 2 micrograms of total RNA using the Nextflex Small RNA-seq kit v3 (Bioo Scientific, batch2) and the NEBNext Small RNA library prep set for Illumina (New England Biolabs, batch1), respectively.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: frontotemporal dementia
characteristics.characteristic: disease
characteristics.text: FTD-C9
characteristics.characteristic: disease staging
characteristics.text: rimod35
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: frontal lobe
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: rimod35
characteristics.characteristic: title