characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-03T12:00:07Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-03T12:00:07Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112467980
characteristics.characteristic: External Id
characteristics.text: MPIPZ
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-03T12:00:07Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-03T12:00:07Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14578029
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12516:Pombe9_Tet_29d
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: fission yeast
characteristics.characteristic: common name
characteristics.text: Protocols: S. pombe cells were inoculated in liquid YES medium overnight at 32°C, diluted to 2-5 x 10exp6 cells/ml the next morning and grown for 6 hours. Next, cells were resuspended in liquid EMM at 1 x 10exp8 cells/ml and 1 ml was treated or not with 20 microliters of EMS (ie 2% final) for 3h in 10 ml tubes at 32°C. Next, cells were washed three times with 5 ml of EMM, resuspended in 1 ml of EMM, plated on YES plates and incubated at 32°C until colonies formed. S. pombe spores were grown to saturation in 1.6 ml of YES medium at 32°C in 96 well plates (2 ml deep well plates). Cells were harvested by centrifugation, washed once with TE and lyzed during two hours at 37°C using 30 units of zymolyase in Y1 Qiagen buffer. Samples were centrifuged at 2,700g for five minutes and supernatants treated with 0.5 mg/ml of RNaseA for 10 minutes at room temperature. DNA was next purified from these lysates using the E-Z 96™ Tissue DNA Kit from Omega Bio-tek following the manufacturer’s instructions except for DNA binding to the columns that was done by gravity flow instead of centrifugation. TPase-based DNA library (>95 samples)
characteristics.characteristic: description
characteristics.text: EMS-treated
characteristics.characteristic: genotype
characteristics.text: Pombe9_Tet_29
characteristics.characteristic: individual
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: organism
characteristics.text: Schizosaccharomyces pombe
characteristics.characteristic: scientific_name
characteristics.text: PR109xPR110
characteristics.characteristic: strain
characteristics.text: Pombe9_Tet_29d
characteristics.characteristic: title