characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-04-25T18:00:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-04-25T18:00:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110141028
characteristics.characteristic: External Id
characteristics.text: Universidad de Leon
characteristics.characteristic: INSDC center name
characteristics.text: 2023-04-25T18:00:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-04-25T18:00:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12239840
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11839:Sample 6
characteristics.characteristic: Submitter Id
characteristics.text: 17
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: sheep
characteristics.characteristic: common name
characteristics.text: Protocols: Samples from perirenal fat perirenal adipose tissue were collected from each lamb after sacrifice at the commercial slaughterhouse. The tissue was preserved in an RNA-stabilization solution (Ambion RNAlater; Life Technologies) and stored at 4 °C for 24 h. After that, the RNA-stabilization solution was removed, and the samples were later frozen at -80 °C until RNA extraction. The RNA extraction was performed using the miRNeasy Mini KIT (Qiagen) modified to use in adipose tissue. Once the RNA was obtained, the cDNA library construction and sequencing were conducted at Novogene in Cambridge (UK) by using the UltraTM RNA Library Prep Kit (NEBNext®).
characteristics.characteristic: description
characteristics.text: suckling lamb
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: GP87_mC_lf_2
characteristics.characteristic: individual
characteristics.text: Ovis aries
characteristics.characteristic: organism
characteristics.text: Ovis aries
characteristics.characteristic: organism
characteristics.text: visceral abdominal adipose tissue
characteristics.characteristic: organism part
characteristics.text: low perirenal fat
characteristics.characteristic: phenotype
characteristics.text: Ovis aries
characteristics.characteristic: scientific name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: Assaf
characteristics.characteristic: strain
characteristics.text: Sample 6
characteristics.characteristic: title