characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-01T00:16:44Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-01T00:16:44Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110141993
characteristics.characteristic: External Id
characteristics.text: CGR, Institute of Infection Veterinary, and Ecological Sciences, University of Liverpool
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-01T00:16:44Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-01T00:16:44Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12240805
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11840:Sample 2
characteristics.characteristic: Submitter Id
characteristics.text: 4
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: horse
characteristics.characteristic: common name
characteristics.text: Protocols: Synovial fluid samples were obtained from both middle carpal joints prior to (day 0), day 28 and 70 days after surgery. Horses were sedated with detomidine (0.01 mg/kg, Domosedan Vet, Orion Pharma Animal Health, Copenhagen, Denmark) and butorphanol tartrate (0.01 mg/kg, Dolorex, MSD Animal Health, Copenhagen, Denmark), and the dorsal surface of the carpi prepared aseptically by chlorhexidine scrubbing and alcohol swabbing. Synovial fluid was aspirated using a 19 gauge 40 mm needle and immediately transferred into ethylene diamine tetra acetic acid (EDTA) tubes (BD Vacutainer, BD A/S, Albertslund, Denmark). Tubes were inverted carefully 5-10 times and samples were further processed as detailed in Table 1 within one hour and stored at -80 ‏⁰C until small RNA sequencing and RT-qPCR analysis of miRNA expression (performed within 6 months). 500 µl SF were treated with a 1ug/µl hyaluronidase (HA) solution to reduce viscosity as preciously described [12]. RNA was extracted from SF using miRNeasy serum/plasma Advanced Kit (Qiagen, Vedbæk, Denmark) according to the manufacturer’s instructions. Samples underwent on-column DNase digestion with RNase free DNase sets (Qiagen, Vedbæk, Denmark) and RNA yield was determined by spectrophotometry (Nano Drop ND-1000 spectrophotometer, Saveen and Werner AB, Limhamn, Sweeden). This project utilized the NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible). The protocol was adapted to accommodate the low input of sample by dilution of adapters 1 in 5 and extra PCR cycles.
characteristics.characteristic: description
characteristics.text: mature
characteristics.characteristic: developmental stage
characteristics.text: H2
characteristics.characteristic: individual
characteristics.text: Equus caballus
characteristics.characteristic: organism
characteristics.text: Equus caballus
characteristics.characteristic: organism
characteristics.text: synovial fluid
characteristics.characteristic: organism part
characteristics.text: midcarpal joint
characteristics.characteristic: sampling site
characteristics.text: Equus caballus
characteristics.characteristic: scientific name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: Sample 2
characteristics.characteristic: title