characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-01T00:17:00Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-01T00:17:00Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111457467
characteristics.characteristic: External Id
characteristics.text: Biogazelle (A CellCarta Company)
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-01T00:17:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-01T00:17:00Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13551503
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12272:MSC_10_PL
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: mesenchymal stem cell of the bone marrow
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Sternal bone marrow aspirates were obtained from healthy donors, following the informed consent. RNA was isolated using the miRNeasy Micro Kit (Qiagen) following the manufacturer instructions. Libraries for mRNA sequencing were prepared using the TruSeq stranded mRNA sample prep kit (Illumina). The starting material (100 ng) of total RNA was mRNA enriched using the oligodT bead system (Illumina). The isolated mRNA was subsequently fragmented using enzymatic fragmentation. Then first strand synthesis and second strand synthesis were performed and the double stranded cDNA was adenylated and Illumina sequencing adaptors ligated onto the fragments ends, and the library was purified (Agencourt AMPure XP). The polyA+ RNA stranded libraries were pre-amplified with PCR and purified (Agencourt AMPure XP). The libraries size distribution was validated and quality inspected on the 2100 Bioanalyzer (high sensitivity DNA chip, Agilent). High quality libraries were quantified using the Qubit Fluorometer (Life Technologies), the concentration normalized and the samples pooled according to the project specification (number of reads).
characteristics.characteristic: description
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: human platelet lysate
characteristics.characteristic: growth condition
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: bone marrow
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: MSC_10_PL
characteristics.characteristic: title