characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-20T16:21:24Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-20T16:21:24Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110463641
characteristics.characteristic: External Id
characteristics.text: Department of General, Visceral and Pediatric Surgery, University Medical Center Gottingen
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-20T16:21:24Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-20T16:21:24Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12561669
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12043:Sample 5
characteristics.characteristic: Submitter Id
characteristics.text: 69
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: replacement histopathological growth pattern
characteristics.characteristic: clinical information
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: The Tissue was collected from the tumor border, capturing both tumor and peritumor healthy tissue per slide. The Visium Spatial Gene Expression for FFPE Kit (10X Genomics, PN-1000338, chemistry v1) was used to generate sequencing libraries. Prior to section placement, tissue adhesion was assured using the Visium Tissue Section Test Slides (10X Genomics, PN-1000347) and RNA-quality of FFPE blocks was assessed using RNeasy FFPE Kit (Qiagen, #73504) for RNA extraction and Agilent 2100 Bioanalyzer system with Agilent RNA 6000 Nano Kit (5067-1511) for determination of DV200. All samples reached DV200 of at least 50%. Nucleic acid extraction was performed using the 10xGenomics Visium Spatial Gene Expression Reagent Kits following the given protocol (CG000239). Nucleic acid library construction was performed using the 10xGenomics Visium Spatial Gene Expression Reagent Kits following the given protocol (CG000239).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: metastatic colorectal cancer
characteristics.characteristic: disease
characteristics.text: PT44
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: mixed neoplasm and adjacent normal tissue
characteristics.characteristic: sampling site
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: Sample 5
characteristics.characteristic: title