characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-14T00:19:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-02-14T00:19:01Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112192483
characteristics.characteristic: External Id
characteristics.text: IGR-PAS
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-14T00:19:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-14T00:19:01Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14303562
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12438:Sample 48
characteristics.characteristic: Submitter Id
characteristics.text: 37
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: MCam48
characteristics.characteristic: cultivar
characteristics.text: Protocols: Flag leaves of barley plants were collected from all experimental variants in two time points (T1, T2) and they were immediately frozen at liquid nitrogen and stored at -80°C until analysis. Experiment was carried out in phytotrons under fully controlled conditions. The 60-70% of air humidity and 234 µmol m-2 s-1 PAR irradiance (Apollo 8 LED Grow Light) were applied. Each pot (H-LSR 4.5 L; 21 cm in diameter and 20 cm in height) was filled with a mixture of loamy soil and peat (3:1, w/w). Ten seeds were sown in each pot and the number of plants was reduced to five after germination. Firstly, all plants were grown at 8ºC/14ºC and 12/12 h (night/day) photoperiod for one month and next at 16ºC/22ºC and 8/16 (night/day) photoperiod until the end of vegetation season under optimal soil moisture, i.e., at above 70% field water capacity (FWC), with some changes in stressed variants. Overall, four environmental variants were applied with two temperature and water regimes: control conditions (C) as described above; drought (D) - soil moisture at 20% FWC and temperature as in C; high temperature (H) - 20ºC/30°C (night/day) and optimal soil moisture; and combination of the stress treatments (HD) - 20ºC/30°C (night/day) and 20% FWC. Abiotic stress was imposed at the flag leaf stage (39 BBCH) and maintained for seven days. Biological samples (flag leaves) for molecular analyses were collected at two time points, i.e., on 3rd (T1) and 7th (T2) day of stress. Soil moisture was controlled gravimetrically by daily weighing of each pot. Total RNA (1-2 µg) was extracted in triplicates using Qiagen RNeasy Plant Mini Kit (Hilden, Germany) according to manufacturer’s instruction. Genomic DNA contamination was removed twice i.e. on-column DNase digestion (RNase-Free DNase Set, Qiagen) and using DNase Max Kit (Qiagen) during and after RNA isolation, respectively. Three flag leaves sampled from different plants of each genotype in a pot formed one replication, and three such replications were examined. Library was constructed using library preparation kit: Illumina TruSeq Stranded mRNA (with poly A selection).
characteristics.characteristic: description
characteristics.text: flag leaf emergence
characteristics.characteristic: developmental stage
characteristics.text: combined drought and heat
characteristics.characteristic: environmental stress
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Hordeum vulgare
characteristics.characteristic: organism
characteristics.text: Hordeum vulgare
characteristics.characteristic: organism
characteristics.text: flag leaf
characteristics.characteristic: organism part
characteristics.text: Hordeum vulgare
characteristics.characteristic: scientific_name
characteristics.text: Sample 48
characteristics.characteristic: title