characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-30T12:21:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-30T12:21:25Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111539382
characteristics.characteristic: External Id
characteristics.text: Fekadu Yadetie, Department of Biological Sciences University of Bergen, Bergen, Norway
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-30T12:21:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-30T12:21:25Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13649446
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12354:Crude oil exposed 3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Polar cod were collected in Southeastern Hinlopen (78° 55’N, 23° 40’E) and transported to the research facility in Kårvika, Norway (69°23’N, 18°10’E). At the research station, the fish were acclimatized. Polar co average weight 81.48 ± 24.76 g (mean±standard deviation) were selected for the experiment. Each fish was anaesthetized in a Finquel® (Tricaine Methanesulfonate; Sigma Aldrich) solution, sexed using ultrasound (General Electric, LOGIQ XP) based on Karlsen & Holm (1994), and marked with a unique dorsal fin tag. Fish were then randomly transferred to one of twelve 300 L tanks (n=36 per tank, 18 males, and 18 females). The fish were maintained in the experimental tanks for three months, from December 15th to April 24th. This period covered late reproductive development (pre-spwning) until post-spawning. Each of the 12 experimental tanks was randomly assigned an oil exposure and a feed ration. Six tanks were exposed to Goliat crude oil, and sex other tanks were maintained under control conditions (no oil). Exposure to a crude oil water accommodated fraction (WAF) was produced by an oiled-rock-column system (Carls et al. 1999), delivering decreasing aqueous levels of oil compounds over time. Fish in each tank were hand fed once daily, with Calanus finmarchicus (CALANUS AS, Lofoten) either at a high (4 % somatic weight day-1) or low-food (1.5 % somatic weight day-1) ration. Samples were taken when polar cod were estimated to be in a late stage of reproductive development (0 days exposure), during the spawning window (47 days exposure) and during the post-spawning period (131 days exposure). At each sampling event, polar cod (n=6-8) were taken from each tank at a balanced sex ratio. Each polar cod was anesthetized before blood was drawn from the caudal vein (Bender et al. 2016). During the second sampling time-point, all polar cod were stripped before the rest of the samples were taken. Fish were then sacrificed by a sharp blow to the head, and fork length (± 0.1 cm), wet weight (± 0.1 g), gonad weight (± 0.1 g), and somatic weight (± 0.1 g) were measured. Samples were taken from the gonad, liver, and gall bladder of each fish. Liver samples were taken and snap-frozen in liquid nitrogen and stored at -80C for RNA extraction. Total RNA was isolated from frozen liver samples as described before (Yadetie et al., 2021), using TRI Reagent according to the manufacturer’s protocol (Sigma-Aldrich, St. Louis, MO, USA). RNA samples from five high-food control and five high-food-oil-exposed female fish from day 47 were submitted for RNA sequencing (RNA-seq) . Sequenced by Novogene (Novogene, Cambridge, UK) on Illumina NovaSeq 6000 System
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Boreogadus saida
characteristics.characteristic: organism
characteristics.text: Boreogadus saida
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Boreogadus saida
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: crude oil
characteristics.characteristic: stimulus
characteristics.text: Crude oil exposed 3
characteristics.characteristic: title