characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-21T16:01:44Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-21T16:01:44Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112203267
characteristics.characteristic: External Id
characteristics.text: Mayo Clinic
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-21T16:01:44Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-21T16:01:44Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14314195
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11739:Bo 103 Veh
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: PDX13 was established from surgically resected tumor tissue from an adult pancreatic ductal adenocarcinoma (PDAC) patient at the Ruhr-University Bochum Comprehensive Cancer Center. Informed and written consent was obtained. Approval by the ethics committee of the Ruhr University Bochum was obtained under Permission no. 3534-9, 3841-10, 16-5792. Experiments were performed according to the guidelines of the local Animal Use and Care Committees at the Ruhr University Bochum (8.87-50.10.32.09.018, 84-02.04.2012.A328 and 81-02.04.2017.A423). Tumor tissues was implanted in NMRInu/nu mice (both flanks). Tumors were expanded and re-implanted for at least three generations. Later, 1-2mm of tumor tissue (early passage) were implanted subcutaneously in 5-10-week-old mice (NMRInu/nu). Treatment was started when tumors were approximately 100-200 mm3 in size. Mice were randomized into vehicle (09.% saline) or Gemcitabine/Paclitaxel (100/30 mg/kg). The integrity of RNA was validated by gel electrophoresis and 500ng were used to make the libraries in triplicates for each condition. RNA was extracted using Trizol Libraries for cells were made using the TruSeq RNA Library Prep Kit V2 (Illumina) according to the manufacturer’s instructions.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: pancreatic ductal adenocarcinoma
characteristics.characteristic: disease
characteristics.text: Bo 103
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: pancreas
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Bo 103 Veh
characteristics.characteristic: title
characteristics.text: subcutaneous injection in NMRInu/nu mice until neoplasm formation
characteristics.characteristic: xenograft