characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-17T10:00:10Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-17T10:00:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111510050
characteristics.characteristic: External Id
characteristics.text: Department of Agriculture, Food, Environment and Forestry (DAGRI) - University of Florence
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-17T10:00:10Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-17T10:00:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13620962
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12332:T1_4
characteristics.characteristic: Submitter Id
characteristics.text: 2
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: 10 plants of L. nobilis were planted in pots using commercial soils collected from two different areas of Altopascio (Lucca, Tuscany, Italy). 5 plants were cultivated in a rural area (named \R\) sparsely inhabited, with significant lower levels of PM (control) compared to 5 plants exposed to higher levels of PM (named \T\; “traffic”, close to a traffic light with a high number of moving cars). Plants were irrigated weekly and routinely inspected to determine any eventual symptoms of biotic and abiotic stresses. Plants remain healthy during the three months of the experimental trial. Healthy, fully-expanded, green leaves were sampled from the 5 plants from each zone at the time of planting (T0; 15th March 2021) and three months after planting (T1; 15th June 2021). Leaves were immediately frozen in liquid nitrogen and stored at - 80 °C until RNA extraction. Plants have a height of around 1.5 meters and were bought in the same plant nursery to reduce plants' physiological and agronomic variability at the beginning of the trial. Frozen leaves were ground to a fine powder. A CTAB-modified protocol was used. The pellet obtained from the extraction procedure was resuspended in 130 µL of nuclease-free water, and the RNAs were treated with DNaseI (NEB). Then, RNAs were purified using a silica-based method (Monarch RNA Cleanup kit - NEB) and eluted twice in 15 µL of nuclease-free water. The 16 transcriptomic libraries were prepared using Illumina mRNA Prep kit, following the manufacturers instructions and a unique dual index combinations was used for each sample for barcoding.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: Altopascio, Lucca, Italy
characteristics.characteristic: environmental history
characteristics.text: traffic air pollution
characteristics.characteristic: environmental stress
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Laurus nobilis
characteristics.characteristic: organism
characteristics.text: Laurus nobilis
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.characteristic: organism part
characteristics.text: Laurus nobilis
characteristics.characteristic: scientific_name
characteristics.text: T1_4
characteristics.characteristic: title