characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-17T00:21:53Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-02-17T00:21:53Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112484152
characteristics.characteristic: External Id
characteristics.text: AstraZeneca, UK
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-17T00:21:53Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-17T00:21:53Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14601501
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12574:Sample 4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: A549
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: A549 cells (CCL-185 on ATCC) were cultured in DMEM medium (Gibco, DMEM, low glucose, GlutaMAX™ Supplement, pyruvate, Catalog number: 21885025) supplemented with 10% heat inactivated Fetal Bovine Serum (FBS), Penicillin (100 U/mL) and Streptomycin (100 U/mL) in a humidified incubator at 37°C; 5% CO2. 10 micromolar miR-200c mimic library or scrambled control. Total RNA from cells was extracted using the RNAdvance Cell kit (Beckman Coulter). RNA extraction was performed using Biomek i7 robotic handler (Beckman Coulter) following manufacturer instructions 55 ng of total RNA were used for RNA-seq library preparation using the KAPA mRNA HyperPrep Kit (Roche) following manufacture’s instruction (KAPA Biosystem, Illumina Platforms) proceeded by TECAN Fluent liquid handing automation system (Tecan). The library was amplified for 14 cycles and quantified using the Fragment Analyzer (Advanced Analytical) with the standard sensitivity NGS kit (Agilent technologies) followed by the library clean-up with 1x volume of AMPure XP beads (Beckman Coulter Life Sciences). All the libraries were pooled and diluted into 1.8 nM quantified using Qubit Fluorometer (ThermoFisher Scientific)
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: lung cancer
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: lung epithelium
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 4
characteristics.characteristic: title