characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-01T00:16:46Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-01T00:16:46Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110256003
characteristics.characteristic: External Id
characteristics.text: School of Biosciences, University of Birmingham
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-01T00:16:46Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-01T00:16:46Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12353620
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11906:LuxO C-terminal 3xFLAG sample A
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: The Vibrio cholerae O1 El Tor strain E7946 was isolated in Brazil (Levine MM, Black RE, Clements ML, Cisneros L, Saah A, Nalin DR, et al. The pathogenicity of nonenterotoxigenic Vibrio cholerae serogroup O1 biotype El Tor isolated from sewage water in Brazil. J Infect Dis. 1982;145: 296-299). Cells were grown in liquid LB minimal medium at 37 degrees with shaking. Immunoprecipitations were done as described by Haycocks et al. (PLoS Pathog. 2015. 11:e1004605) using Vibrio cholerae strain E7946. After growing cells to midlog phase, crosslinking, and sonication, protein DNA complexes were immunoprecipitated using anti-FLAG. Libraries were prepared using immunoprecipited protein-DNA complexes immobilised with Protein A-sepharose. DNA fragments were then blunt ended, A-tailed and barcoded. This was done using an NEB quick blunting and ligation kit, the Klenow fragment (5’-3’ exo-, NEB) and NEXTflex ChIP-seq barcodes (Bioo Scientific). Following elution of complexes from the Protein-A sepharose, crosslinks were reversed and barcoded libraries were amplified by PCR. The number of PCR cycles was determined empirically for each library. After amplification, library concentration was quantified using Qubit analysis and real time PCR.
characteristics.characteristic: description
characteristics.text: mid logarithmic growth
characteristics.characteristic: developmental stage
characteristics.text: pAMCFluxO
characteristics.characteristic: genotype
characteristics.text: Vibrio cholerae
characteristics.characteristic: organism
characteristics.text: Vibrio cholerae
characteristics.characteristic: organism
characteristics.text: nucleoid
characteristics.characteristic: organism part
characteristics.text: Vibrio cholerae
characteristics.characteristic: scientific name
characteristics.text: E7946
characteristics.characteristic: strain
characteristics.text: LuxO C-terminal 3xFLAG sample A
characteristics.characteristic: title