characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-21T10:22:56Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-02-21T10:22:56Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112652175
characteristics.characteristic: External Id
characteristics.text: Garvan Institute of Medical Research
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-21T10:22:56Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-21T10:22:56Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14649886
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12642:Sample 3
characteristics.characteristic: Submitter Id
characteristics.text: 10
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: neutrophil
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: AT-3 tumor cells were injected into ear pinnae of 8 weeks old C57BL/6 female and male mice (0.3x106 cells in 5μl PBS). Ten days after tumor cell inoculation, mice were divided into 3 groups and either left untreated or were treated with 20×10^6 S. aureus bioparticles or saline vehicle intratumorally, and sacrificed 24h later. Tumors were dissected, placed in 2ml PBS+2%BSA and gently disaggregated through a 100μm cell strainer using a 1ml syringe plunger. Cells were collected, centrifuged and resuspended in 5ml PBS+ 2%BSA. Single-cell suspensions were stained for 30 min at 4°C with Zombie UV™ Fixable Viability kit (Biolegend cat#423107), washed and resuspended in 50ul PBS+1%FBS with fluorophore-conjugated antibodies (FITC-conjugated anti-Ly6G, PeCy7-conjugated anti-CD45, APCCy7-conjugated-anti-CD11b), stained again for 30 min at 4°C, filtered through 100μm cell strainers, and sorted using a BD FACSymphony cell sorter (BD Biosciences) into PBS+2%BSA. The cell preparation time before loading onto the BD Rhapsody was <3h. Cell viability and counting were evaluated with the BD Rhapsody™ Scanner following a standard protocol provided by the manufacturer (BD Biosciences), and samples with viabilities >75% were used for sequencing. Libraries were constructed using the BD Rhapsody WTA Amp Kit (BD Biosciences, cat#633801). Libraries were constructed using the BD Rhapsody WTA Amp Kit (BD Biosciences, cat#633801).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: AT-3 injected into ear pinnae until neoplasm formation
characteristics.characteristic: growth condition
characteristics.text: Ly6G+CD11b+
characteristics.characteristic: immunophenotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: pinna
characteristics.characteristic: organism part
characteristics.text: S. aureus bioparticles (stimulated)
characteristics.characteristic: phenotype
characteristics.text: neoplasm
characteristics.characteristic: sampling site
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: mixed
characteristics.characteristic: sex
characteristics.text: Staphylococcus aureus bioparticles
characteristics.characteristic: stimulus
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: Sample 3
characteristics.characteristic: title