characteristics.text: 2024-02-23
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: SAMEA112328270
characteristics.characteristic: External Id
characteristics.text: San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute, Milan, Italy
characteristics.characteristic: INSDC center name
characteristics.text: 2023-01-14T18:41:26Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14439185
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12508:Lung_KPneo_control
characteristics.characteristic: Submitter Id
characteristics.text: 8
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: leukocyte
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Lungs were harvested at day 9 and digested to obtain single cell suspensions to be processed for scRNA analysis. CD45+ cells were isolated using ARIA Cell Sorter (BD). Single cell suspensions were processed for single cell analysis using Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) (10X Genomics) following manufacturer’s instructions. 8-weeks females C57/BL6 mice (4 mice per group) were injected with 5x10^4 KPneo cells intravenously. Lungs were harvested at day 9 post-inoculation. Mice were administered 30 ug of recombinant Flt3L on day -2, 2, 4 , 6. On day 5, mice received a single dose of aCD40 (200ug intraperitoneally). Control mice received intraperitoneal injections of PBS on day -2,2,4,6 and isotype rat aIgG on day 6. Nucleic acids (polyA RNA) were extracted during single cell encapsulation and immediately barcoded, according to Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) (10X Genomics) protocol. Libraries were constructed using Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) (10X Genomics) protocol and following manufacturer’s instructions.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: KrasG12D/WT; Tp53
characteristics.characteristic: genotype
characteristics.text: CD45 positive
characteristics.characteristic: immunophenotype
characteristics.text: pool of 4 mice
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: lung
characteristics.characteristic: organism part
characteristics.text: neoplasm
characteristics.characteristic: sampling site
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: C57BL/6JO
characteristics.characteristic: strain
characteristics.text: Lung_KPneo_control
characteristics.characteristic: title