characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-28T00:17:18Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-02-28T00:17:18Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112184279
characteristics.characteristic: External Id
characteristics.text: Laboratory of Plant Physiology, Plant Sciences Group, Wageningen University& Research
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-28T00:17:18Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-28T00:17:18Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14294170
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12424:0mM-0h-1
characteristics.characteristic: Submitter Id
characteristics.text: 4
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Root materials were harvested by razor blades from S. parvula seedlings grown for 4 days on ½ MS agar plates and transferred on mesh to new medium supplemented with 0 mM or 175 mM NaCl for 0 h, 3 h, 24 h or 48 h. Seeds of Schrenkiella parvula (Lake Tuz ecotype) were stratified in dark at 4°C for 7 days and were surface-sterilized in 30% (v/v) sodium hypochlorite and washed 4 times with H2O. Seeds were geminated on nylon mesh strips on ½-strength Murashige-Skoog (½ MS) medium including vitamins (Duchefa), supplemented with 0.1% 2-(N-morpholino) ethanesulfonic acid (MES, Duchefa), 1% Daishin agar (Duchefa) and pH was adjusted with KOH to 5.75. Plates were placed at 70° racks in the growth chamber with 16 h light:8 h dark, 130 μmol m-2 s-1 LED light, 21°C and 70% relative humidity. Samples were frozen in liquid nitrogen and ground before RNA extraction. Total RNA was isolated using NZY Total RNA Isolation kit (NZYtech). RNA library preparation was conducted by Novogene Co., LTD (United Kingdom)
characteristics.characteristic: description
characteristics.text: seedling development stage
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: MS agar
characteristics.characteristic: growth condition
characteristics.text: Schrenkiella parvula
characteristics.characteristic: organism
characteristics.text: Schrenkiella parvula
characteristics.characteristic: organism
characteristics.text: root
characteristics.characteristic: organism part
characteristics.text: Schrenkiella parvula
characteristics.characteristic: scientific_name
characteristics.text: 0mM-0h-1
characteristics.characteristic: title