characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-22T09:00:26Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-02-22T09:00:26Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110464435
characteristics.characteristic: External Id
characteristics.text: LabMedMolGE
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-22T09:00:26Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-22T09:00:26Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12562463
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12032:Sample 2
characteristics.characteristic: Submitter Id
characteristics.text: 61
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not relapsed
characteristics.characteristic: clinical history
characteristics.text: Group0
characteristics.characteristic: cohort
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: We retrospectively reviewed a cohort of 156 patients with stageIII IVB (AJCC TNM 7 th Ed.) OPSCC. Forty patients were diagnosed and treated at Treviso Regional Hospital, and 116 at National Cancer Institute of Aviano and/or Pordenone Hospital. All patients were managed with curative intent with elective radiotherapy ± chemotherapy or surgery ±adjuvant (chemo)radiotherapy, in accordance with current clinical practice. Genomic DNA was extracted from OPSCC formalin-fixed paraffin-embedded tissues. The quantification of HPV16DNA was performed by real-time quantitative PCR by using specific primers for the amplification of a region spanning the E6 genes of the HPV16 genome, whereas the methylation status of LINE1repetitive sequences was evaluated by real-time quantitative Methylation-Specific PCR. RNA extraction and purification from treated cells were performed using SNET lysis buffer (10 mM Tris pH 8.0, 0.1M EDTA, 0.5% sodium dodecyl sulphate) and phenol/chloroform/isoamyl Nucleic acids were quantified with Qubit 2.0 fluorimeter using Qubit RNA HS assay kit (Termo Fisher Scientifc, USA) and the assessment of nucleic acids integrity (RNA Integrity Number) was performed with Agilent 4150 TapeStation System (Agilent Technologies, USA). Libraries preparation for transcriptome analysis was performed employing the TruSeq RNA Exome kit (Cat.20020189, Illumina) for FFPE samples starting from 200 of RNA as input materials, respectively, according to manufacturersprotocols
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: oropharyngeal squamous cell carcinoma
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: oropharynx
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: Sample 2
characteristics.characteristic: title