characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-26T00:18:22Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-02-26T00:18:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112651948
characteristics.characteristic: External Id
characteristics.text: German Center for Neurodegenerative Diseases (DZNE)
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-26T00:18:22Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-26T00:18:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14649656
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12646:rimod15
characteristics.characteristic: Submitter Id
characteristics.text: 70
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: The brain samples and/or bio samples were obtained from The Netherlands Brain Bank, Netherlands Institute for Neuroscience, Amsterdam (open access: www.brainbank.nl). All Material has been collected from donors for or from whom a written informed consent for a brain autopsy and the use of the material and clinical information for research purposes had been obtained by the NBB. Additional samples were provided by the Queen Square Brain Bank of Neurological Disorders and MRC, King College London. We complied to all relevant regulations of the mentioned brain banks for the work with these samples. Total RNA for CAGE-seq was isolated from ±100mg of frozen brain tissue with TRIzol reagent (Thermo Fischer Scientific) according to the manufacturer recommendation, followed by purification with the RNeasy mini columns (Qiagen) after DNAse treatment. CAGE-seq libraries were prepared from 5 micrograms of RNA from frozen brain tissues according to a published protocol:Takahashi, H., Lassmann, T., Murata, M. & Carninci, P. 5′ end-centered expression profiling using cap-analysis gene expression and next-generation sequencing. Nat. Protoc. 7, 542-561 (2012).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: frontotemporal dementia
characteristics.characteristic: disease
characteristics.text: FTD-C9
characteristics.characteristic: disease staging
characteristics.text: rimod35
characteristics.characteristic: individual
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: frontal lobe
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: rimod15
characteristics.characteristic: title