characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-31T00:22:11Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-31T00:22:11Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111329107
characteristics.characteristic: External Id
characteristics.text: Department of Pathophysiology, Osaka Metropolitan University Graduate School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-31T00:22:11Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-31T00:22:11Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13426672
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11992:vehicle 2 day 15
characteristics.characteristic: Submitter Id
characteristics.text: 7
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Female C57BL/6J mice were purchased from Charles River Laboratories At 7 weeks old, back hair was shaved and steroid (5 μg fluocinolone acetonide in 200 μl acetone) or vehicle were topically topically applied once every three days for 12 days and the skin samples were collected 1 day after the treatment. The back skin collected from mouse was immediately frozen in liquid nitrogen and was sliced into sections using a cryostat in order to promote the permeation of RNA isolation reagent. RNA was extracted using RNAiso Plus reagent (Takara). Libraries were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit with the NEBNext Poly(A) mRNA Magnetic Isolation Module.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: dorsal skin
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: vehicle 2 day 15
characteristics.characteristic: title