characteristics.text: ERC000015
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-09T08:17:48Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-09T08:17:48Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111438055
characteristics.characteristic: External Id
characteristics.text: Max Rubner-Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-09T08:17:48Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-09T08:17:48Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13531477
characteristics.characteristic: SRA accession
characteristics.text: ernst9523A
characteristics.characteristic: Submitter Id
characteristics.text: fwd:AATGATACGGCGACCACCGAGATCTACACNNNNNNNNTATGGTAATTGT::rev:CAAGCAGAAGACGGCATACGAGATNNNNNNNNAAGTCGAGAGTCAGTCAGCC
characteristics.characteristic: adapters
characteristics.text: 0.2
characteristics.unit: g
characteristics.characteristic: amount or size of sample collected
characteristics.text: ENVO_01001033:digestive tract environment
characteristics.characteristic: broad-scale environmental context
characteristics.text: 2018-11-28
characteristics.characteristic: collection date
characteristics.text: stool sample
characteristics.characteristic: description
characteristics.text: ENVO_00002003:fecal material
characteristics.characteristic: environmental medium
characteristics.text: Germany
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 49.0139822
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 8.4240071
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: 38
characteristics.unit: year
characteristics.characteristic: host age
characteristics.text: Feces
characteristics.characteristic: host body product
characteristics.text: 28.5
characteristics.unit: kg/m2
characteristics.characteristic: host body-mass index
characteristics.text: male
characteristics.characteristic: host sex
characteristics.text: human-gut
characteristics.characteristic: human gut environmental package
characteristics.text: metagenome
characteristics.characteristic: investigation type
characteristics.text: ENVO_2100002:intestine environment
characteristics.characteristic: local environmental context
characteristics.text: PCR
characteristics.characteristic: nucleic acid amplification
characteristics.text: Nucleospin Stool Kit (Macherey Nagel)
characteristics.characteristic: nucleic acid extraction
characteristics.text: human gut metagenome
characteristics.characteristic: organism
characteristics.text: initial denaturation:98degC_2min; 25 cycles: denaturation:98degC_10s; annealing:55degC_30s; extension:72degC_15s; final_extension:72degC_5min
characteristics.characteristic: pcr conditions
characteristics.text: fwd:GTGYCAGCMGCCGCGGTAA::rev:GGACTACNVGGGTWTCTAAT
characteristics.characteristic: pcr primers
characteristics.text: ErNst_study
characteristics.characteristic: project name
characteristics.text: freezing
characteristics.characteristic: sample material processing
characteristics.text: -80
characteristics.unit: °C
characteristics.characteristic: sample storage temperature
characteristics.text: human gut metagenome
characteristics.characteristic: scientific_name
characteristics.text: Illumina MiSeq v3
characteristics.characteristic: sequencing method
characteristics.text: 16S-rRNA
characteristics.characteristic: target gene
characteristics.text: V4
characteristics.characteristic: target subfragment
characteristics.text: ernst9523A
characteristics.characteristic: title