characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-01T00:17:12Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-01T00:17:12Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112648658
characteristics.characteristic: External Id
characteristics.text: Associate Professor, PI
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-01T00:17:12Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-01T00:17:12Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14646378
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12652:E3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Collection of PAO1 cells following treatment by compounds Overnight cultures were sub-cultured and grown to mid-log phase to OD=0.4. Dissolved in antibiotic solution in Mueller-Hinton Broth (MHB) to a final concentration of OD=0.2, MIC=1X, and final volume of 500uL. Samples incubated at 37C statically for 30 mins. 1mL of RNA protect reagent added to 500uL of sample. Cells were lysed via treatment with TE buffer containing 15mg/mL lysozyme for 10 mins with frequent vortexing, following manufacturer's instructions Directional mRNA library preparation (no ribosomal RNA depletion). Following fragmentation, cDNA was synthesized using random hexamer primers and then the dUTPs were replaced with dTTPs. Finally, the following was performed: end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Mueller-Hinton Broth
characteristics.characteristic: growth condition
characteristics.text: Pseudomonas aeruginosa PAO1
characteristics.characteristic: organism
characteristics.text: Pseudomonas aeruginosa PAO1
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Pseudomonas aeruginosa PAO1
characteristics.characteristic: scientific_name
characteristics.text: PAO1
characteristics.characteristic: strain
characteristics.text: E3
characteristics.characteristic: title