characteristics.text: ERC000036
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-22T15:31:26Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-04-05T18:37:14Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112672614
characteristics.characteristic: External Id
characteristics.text: Swedish University of Agricultural Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-22T15:31:26Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-04-05T18:37:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14670166
characteristics.characteristic: SRA accession
characteristics.text: seec_wastewater_is_172_021
characteristics.characteristic: Submitter Id
characteristics.text: 0.5
characteristics.unit: L
characteristics.characteristic: amount or size of sample collected
characteristics.text: not provided
characteristics.characteristic: area of sampling site
characteristics.text: 2021-12-06
characteristics.characteristic: collection date
characteristics.text: Untreated wastewater was collected in Stockholm at Käppalaverket, Bromma reningsverk and Henriksdal reningsverk on 2021-12-06 over 24 hours using flow compensated samplers. The samples were then combined flow-proportionally into a composite sample for the city. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer’s protocol (Promega, # TM663 Rev 4/22) at SEEC-KTH. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer’s protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer’s protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.
characteristics.characteristic: description
characteristics.text: Sweden
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: Stockholm
characteristics.characteristic: geographic location (region and locality)
characteristics.text: virus
characteristics.characteristic: investigation type
characteristics.text: Käppalaverket, Bromma reningsverk and Henriksdal reningsverk
characteristics.characteristic: name of the sampling site
characteristics.text: Ion AmpliSeq SARS-CoV-2 Insight Research Assay with SuperScript™ VILO™ cDNA Synthesis Kit using Ion Chef™ (Thermo Fisher, # A51306, # 11754050, # MAN0019277, Rev B.0, pp. 3-4, # MAN0024915 Rev B.0, Ch 4, pp. 42-43)
characteristics.characteristic: nucleic acid amplification
characteristics.text: TNA concentration using Maxwell RSC Enviro Total Nucleic Acid Kit (Promega, # AS1831 & # TM663 Rev 4/22)
characteristics.characteristic: nucleic acid extraction
characteristics.text: wastewater metagenome
characteristics.characteristic: organism
characteristics.text: 1720000
characteristics.characteristic: population size of the catchment area
characteristics.text: 2021-12-08
characteristics.characteristic: receipt date
characteristics.text: 1
characteristics.unit: day
characteristics.characteristic: sample storage duration
characteristics.text: not provided
characteristics.characteristic: sample storage location
characteristics.text: 2021-12-07
characteristics.characteristic: sample transportation date
characteristics.text: 1
characteristics.unit: day
characteristics.characteristic: sample transportation time
characteristics.text: 24 hour period, using a flow compensated sampler at each site, flow-proportionally combined into a single sample
characteristics.characteristic: sampling time point
characteristics.text: wastewater metagenome
characteristics.characteristic: scientific_name
characteristics.text: wastewater treatment plant (municipal)
characteristics.characteristic: sewage type
characteristics.text: not provided
characteristics.characteristic: size of the catchment area
characteristics.text: 2697049
characteristics.characteristic: surveillance target
characteristics.text: Severe acute respiratory syndrome coronavirus 2
characteristics.characteristic: surveillance target (scientific name)
characteristics.text: 2021-W49 Stockholm Amplified SARS-CoV-2 RNA from wastewater
characteristics.characteristic: title