characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-23T10:00:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-02-23T10:00:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112474168
characteristics.characteristic: External Id
characteristics.text: Wallenberg Center for Molecular Medicine (WCMM) Department of Biomedical and Clinical Sciences (BKV) Division of Molecular Medicine and Virology (MMV) Faculty of Medicine and Health Sciences, Linkoping University, Linkoping, Sweden
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-23T10:00:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-23T10:00:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14584221
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12598:hESC_Chir3d
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: embryonic cell line
characteristics.characteristic: cell_line
characteristics.text: embryonic stem cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were detached after 4 hours, 24 hours, and 72 hours of CHIR treatment (n = 6 wells per time point). The cell suspensions were filtered through a 40 mm cell strainer (KKE3.1, Carl Roth), and cell concentrations were evaluated using a hemocytometer in a Leica DM 204 IL LED microscope and adjusted to 1’000 cells/µl. hESCs were cultured in Essential 8TM medium (A1517001, ThermoFisher Scientific) in plates and flasks coated with 10 mg/ml Vitronectin (A14700, ThermoFisher Scientific). Cells were passaged by incubation in 0.5 mM EDTA-PBS (stock: 0.5 M EDTA, AM9260G, Thermo Fisher Scientific). Cells were treated with 10 mM GSK-3 inhibitor CHIR99021 (SML1046-25MG, Sigma-Aldrich) for 4 hours, 24 hours, and 72 hours (n = 6 wells per time point). Single cells were run in a 10X Chromium Controller and samples were further processed according to the manufacturer’s indications (10X Genomics; v3 protocol). Library preparation was performed according to the manufacturer’s indications (10X Genomics; v3 protocol)
characteristics.characteristic: description
characteristics.text: embryo stage
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: inner cell mass
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: hESC_Chir3d
characteristics.characteristic: title