characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-17T00:21:53Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-02-17T00:21:53Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112483524
characteristics.characteristic: External Id
characteristics.text: Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai0, China, 20001.
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-17T00:21:53Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-17T00:21:53Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14600875
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12569:IL-4-2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: in vitro derived B cells
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mouse splenic B cells were purified by negative selection and cultured in complete medium in the presence of in vitro differentiation cocktails (anti-IgM, R848, anti-CD40, IL-21 plus IFN-γ or IL-4) for 3 days. The in vitro dereived B cells were collected for RNA sequencing. The in vitro indued B cells were resuspended in TRIzol reagent for total RNA extraction. RNA integrity was assessed using the Fragment Analyzer 5400 (Agilent Technologies, CA, USA). Then total RNA was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations. The library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
characteristics.characteristic: description
characteristics.text: IL-4 stimulation condition
characteristics.characteristic: growth condition
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: C57
characteristics.characteristic: strain
characteristics.text: IL-4-2
characteristics.characteristic: title