characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-21T04:22:04Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-21T04:22:04Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112352729
characteristics.characteristic: External Id
characteristics.text: UT SOUTHWESTERN
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-21T04:22:04Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-21T04:22:04Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14463626
characteristics.characteristic: SRA accession
characteristics.text: Patient_MDA7_Relapse_3_IDH2
characteristics.characteristic: Submitter Id
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Targeted sequencing of IDH1 and IDH2 genes was performed using bone marrow specimens from matched diagnosis and relapsed AML patients with IDH baseline mutation before or after IDHmut inhibitor treatment. gDNA was extracted from the bone marrow clot sections using the QuickExtract FFPE DNA extraction kit (Epicenter Biotechnologies) following the manufacturer's instructions. The exon sequences of IDH1 and IDH2 genes were PCR amplified. The DNA fragments for each exon were purified with QIAquick spin columns (Qiagen), mixed at equal molarity, and fragmented using Covaris acoustic shearing following the manufacturer�s instructions. Sonicated DNA was processed for library generation using NEBNext Ultra II DNA Kits (New England Biolabs) following the manufacturer�s protocol. Libraries with different index sequences were quantified, pooled, and sequenced on an Illumina Nextseq500 system using the 75bp high output sequencing kit.
characteristics.characteristic: description
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Targeted sequencing of IDH1 and IDH2 genes in AML samples
characteristics.characteristic: title