characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-01T00:17:11Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-01T00:17:11Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112635190
characteristics.characteristic: External Id
characteristics.text: University of Cologne, Faculty of Medicine and University Hospital Cologne, Department I of Internal Medicine, Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf, 50931 Cologne, Germany
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-01T00:17:11Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-01T00:17:11Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14627170
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12629:KO9-CLL#2
characteristics.characteristic: Submitter Id
characteristics.text: 30
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HS-5
characteristics.characteristic: cell_line
characteristics.text: stromal cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: LYN WT and LYN KO cells (2 clones per genotype) were cultured with primary CLL patient samples (3 different patients) for 72h. CLL cells were removed, feeder layers washed carefully before trypsinization and subsequently contaminating CLL cells were removed by magnetic depletion of CD45+ cells using human CD45 MojoSort reagents (Biolegend) according to the manufacturer’s instructions. mRNA was isolated HS-5 cells using the RNeasy plus kit (Qiagen) according to the manufacturer’s instructions and RNA quantity and quality was examined by NanoDrop1000 (Thermo Scientific) spectrometric measurement. Samples were processed and sequenced PE100, 50M reads per sample at the Cologne Center for Genomics according to in-house standards.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: chronic leukemia
characteristics.characteristic: disease
characteristics.text: LYN knockout
characteristics.characteristic: genotype
characteristics.text: co-culture with chronic lymphocytic leukemia cells from patient_2
characteristics.characteristic: growth condition
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: bone marrow
characteristics.characteristic: organism part
characteristics.text: LYN deficiency
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: KO9-CLL#2
characteristics.characteristic: title