characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-04-02T00:17:29Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-04-02T00:17:29Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112146512
characteristics.characteristic: External Id
characteristics.text: Innovent Biologics (Suzhou) Co. Ltd
characteristics.characteristic: INSDC center name
characteristics.text: 2023-04-02T00:17:29Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-04-02T00:17:29Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14256420
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12373:IL2_Non_Alpha_1
characteristics.characteristic: Submitter Id
characteristics.text: 6 to 8
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: leukocyte
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Five days after the last dose (3mg/kg) of IgG, IL-2wt and IL-2nα mutein, MC38 tumor (four mice from each group) were dissected from mice and digested as above mentioned. Tumor-infiltrating leukocytes (TILs) were isolated by Percoll gradient and CD45+ leukocytes were enriched by CD45 (TIL) MicroBeads (Miltenyi, 130-110-618). Samples from the same group were labeled with different TotalSeq™ anti-mouse Hashtag (Biolegend) for 10x Genomics platform. Three samples from each group with higher cell viability were selected. 10,000 cells from each sample were mixed into a single sample and resuspended with a concentration of 1,000 cells/ul. Single-cell RNAseq library was constructed using the Chromium Next GEM Single Cell 3’ Reagent Kits v3.1. Single cell suspensions were first partitioned into nanoliter-scale Gel Beads-in-emulsion (GEMs). Full-length cDNA tagged with a 16nt 10x barcode and barcoded DNA from the cell surface protein Feature Barcode were generated within each individual GEM during RT reaction. Purified DNA after RT reaction was amplified for 11 cycles and size selection was used to separate the amplified cDNA molecules for 3ʹ Gene Expression and Cell Surface Protein library construction.
characteristics.characteristic: description
characteristics.text: colon adenocarcinoma
characteristics.characteristic: disease
characteristics.text: CD45-positive
characteristics.characteristic: immunophenotype
characteristics.text: pool of four mice
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: neoplasm
characteristics.characteristic: sampling site
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: C57BL/6NJ
characteristics.characteristic: strain
characteristics.text: IL2_Non_Alpha_1
characteristics.characteristic: title
characteristics.text: MC-38
characteristics.characteristic: xenograft