characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-24T07:00:06Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-24T07:00:06Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112246066
characteristics.characteristic: External Id
characteristics.text: Department of Agriculture, Food, Environment and Forestry (DAGRI) - University of Florence
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-24T07:00:06Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-24T07:00:06Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14356938
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12477:T1_4
characteristics.characteristic: Submitter Id
characteristics.text: 2
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Dress
characteristics.characteristic: cultivar
characteristics.text: Protocols: 10 plants of Photinia x fraseri Dress were planted in pots using commercial soils collected from two different areas of Altopascio (Lucca, Tuscany, Italy). 5 plants were cultivated in a rural area (named \R\) sparsely inhabited, with significant lower levels of PM (control) compared to 5 plants exposed to higher levels of PM (named \T\; “traffic”, close to a traffic light with a high number of moving cars). Plants were irrigated weekly and routinely inspected to determine any eventual symptoms of biotic and abiotic stresses. Plants remain healthy during the three months of the experimental trial. Healthy, fully-expanded, green leaves were sampled from the 5 plants from each zone at the time of planting (T0; 15th March 2021) and three months after planting (T1; 15th June 2021). Leaves were immediately frozen in liquid nitrogen and stored at -80 °C until RNA extraction. Plants have a height of around 1.5 meters and were bought in the same plant nursery to reduce plants' physiological and agronomic variability at the beginning of the trial. Frozen leaves were grounded in liquid nitrogen and a CTAB-modified protocol was used (Doyle & Doyle, 1987) to extract RNA from the tissue powder. The RNAs were eluted in 130 µL of nuclease-free water, treated with DNaseI (NEB) and purified using specific silica-based columns (Monarch RNA Cleanup kit - NEB). Qualitative and quantitative check of the nucleic acid extracted were performed through Agilent 2100 Bioanalyzer (kit RNA nano - Agilent Technologies) and Qubit™ 4 Fluorometer (kit RNA BR - Thermo Fisher Scientific) respectively. Each sample was converted into sequencing libraries following the procedure of Stranded mRNA Library Prep (Illumina) and a combination of exclusive unique dual index. The concentration of each of the libraries was determined using Qubit™ 4 Fluorometer (dsDNA High Sensitivity Kit -Invitrogen).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: traffic (polluted)
characteristics.characteristic: growth condition
characteristics.text: Photinia x fraseri
characteristics.characteristic: organism
characteristics.text: Photinia x fraseri
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.characteristic: organism part
characteristics.text: Photinia x fraseri
characteristics.characteristic: scientific_name
characteristics.text: T1_4
characteristics.characteristic: title