characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-19T00:22:56Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-02-19T00:22:56Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112623590
characteristics.characteristic: External Id
characteristics.text: WIS
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-19T00:22:56Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-19T00:22:56Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14615573
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12620:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: 2
characteristics.unit: month
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: adipose macrophage
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Femora and tibiae, irradiated and non irradiated were isolated from two months DNMT3AMut, bone marrow was flushed in to 2 tubes. We have adopted a protocol developed by Rosen et.al. The protocol combines a density gradient centrifugation method optimized for adipose tissue followed by nuclei “cleanup” step by sorting. Nuclei were sorted to low binding Eppendorf tubes and library was prepared by the 10X genomics V3 3’ kit. Libraries were prepared by the 10X genomics V3 3’ kit.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: NOD/SCID/IL-2Rgc-null
characteristics.characteristic: genotype
characteristics.text: IRbadge_CD45neg
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: bone marrow
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: Sample 1
characteristics.characteristic: title