characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-23T00:28:37Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-02-23T00:28:37Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112641172
characteristics.characteristic: External Id
characteristics.text: Department of Biological Sciences University of Bergen, Bergen, Norway
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-23T00:28:37Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-23T00:28:37Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14633147
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12605:C48-4
characteristics.characteristic: Submitter Id
characteristics.text: 1.5
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Atlantic cod
characteristics.characteristic: common name
characteristics.text: Protocols: A total of 5 males juvenile cod (G. morhua) were used for PCLS (n = 5/group). PCLS preparation and chemical exposure in the culture was performed as previously described (Yadetie et al., 2021 et al., 2021). Atlantic cod liver slices were cultured in 24-well plates in the growth medium containing DMSO (vehicle control) or EE2 (10 nM). For time response study, the slices were collected at 12, 24 and 48 h after exposure to DMSO control or 10nM EE2 in the culture. The slices were snap frozen in liquid nitrogen and stored at -80 °C for RNA extraction. RNA extraction was performed from a total of 30 samples comprising 6 groups (three time points with 10 nM EE2 and time-matched control groups, n = 5/group). RNA extraction was performed using TRIreagent following the manufacturers protocols (Sigma-Aldrich Oslo, Norway). Poly(A) pluss RNA sequenced on Illumina HiSeq 4000 system (50 million 2x75 bp paired-end option)at Genomic Core Facility, University of Bergen, Norway
characteristics.characteristic: description
characteristics.text: juvenile stage
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: 4
characteristics.characteristic: individual
characteristics.text: Gadus morhua
characteristics.characteristic: organism
characteristics.text: Gadus morhua
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Gadus morhua
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C48-4
characteristics.characteristic: title