characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-14T00:18:55Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-02-14T00:18:55Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111539387
characteristics.characteristic: External Id
characteristics.text: Departamento de Produccion Animal, Facultad de Veterinaria, Universidad de Leon
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-14T00:18:55Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-14T00:18:55Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13649451
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12355:Sample 11
characteristics.characteristic: Submitter Id
characteristics.text: Assaf
characteristics.characteristic: breed
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: mammary epithelial cell
characteristics.characteristic: cell_type
characteristics.text: sheep
characteristics.characteristic: common name
characteristics.text: Protocols: Sheep with extreme FEI values (8 high feed efficiency sheep (H-FE), FEI = −0.29 (SD = 0.23), RFI = −0.16 (SD = 0.25), and 8 low feed efficiency sheep (L-FE), FEI = 0.81 (SD = 0.24), RFI = 0.19 (SD = 0.24)) were sampled for RNA-Seq. To summarize, 50 mL of fresh milk was collected from each animal one hour after milking and 10 min after injection of 5 IU of oxytocin/animal (Facilpart, Laboratorios SYVA, León, Spain) to maximize milk somatic cell concentration. To prevent RNA degradation, udders were cleaned with soap and water and disinfected with povidone-iodine, and the nipples were also flushed with RNAseZap (Ambion, Austin, TX, USA). A sterile gauze was used to cover the collection tube to avoid contamination. For RNA extraction, the milk somatic cells (MSC) were pelleted by centrifugation at 650×g for 10 min at 4 °C in the presence of a final concentration of 0.5 mM of EDTA. Then, the pellet was washed twice with 10 and 2 mL of PBS (pH 7.2 and 0.5 mM of EDTA) followed by centrifugation at 650×g for 10 min at 4 °C. The last pellet was kept in RNAlater (Sigma-Aldrich, Madrid, Spain) and stored at −80 ºC until RNA extraction using 500 μL of TRIzol according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA, USA). The RNA sequencing was conducted at CNAG (Centro Nacional de Análisis Genómico, Barcelona, Spain), where the TrueSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA, USA) was used for library preparation.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Ovis aries
characteristics.characteristic: organism
characteristics.text: Ovis aries
characteristics.characteristic: organism
characteristics.text: mammary gland
characteristics.characteristic: organism part
characteristics.text: milk
characteristics.characteristic: organism substance
characteristics.text: low feed efficiency
characteristics.characteristic: phenotype
characteristics.text: Ovis aries
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: Sample 11
characteristics.characteristic: title