characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-17T16:00:06Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-17T16:00:06Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112484179
characteristics.characteristic: External Id
characteristics.text: VANDERBILT UNIVERSITY
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-17T16:00:06Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-17T16:00:06Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14601526
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12576:ctl_chr_HA
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: MIN6
characteristics.characteristic: cell_line
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: HA-ZFP92 lentivirus-infected MIN6 cells were trypsinized and washed 2 times with 1%BSA in PBS solution(150x103 cells were used per pull-down). The CUT&RUN (Cleavage Under Targets and Release Using Nuclease) assay was performed by using the CUT&RUN Assay KIT (#86652 Cell Signaling Technology) following the manufacturer’s protocol. For anti-HA-ZFP92 pulldowns from MIN6 cells, rabbit anti-HA antibodies (#3724) and normal rabbit control IgGs from the kit were used (all antibodies are from Cell Signaling). Briefly, buffer-washed cells were bound to Concanavalin A-coated magnetic beads, permeabilized with 0.05% digitonin buffer, incubated with antibodies at 4°C overnight, washed twice with digitonin buffer, and incubated with Protein A-MNase (pA-MN) for 1 hour at 4°C. After two washes, cell-bound beads were incubated for 30 min at 4°C in 2 mM CaCl2 for pA-MN digestion, then incubated with RNAse-containing Stop Buffer (30 min. at 37°C) to release DNA. DNA was further purified using spin columns (14209S, Cell Signaling) and directly used for library prep or qPCR. DNA was processed through the ChIP-seq library preparation
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: insulinoma
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: pancreas
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: ctl_chr_HA
characteristics.characteristic: title