characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-02-17T00:21:52Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-02-17T00:21:52Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112483363
characteristics.characteristic: External Id
characteristics.text: Researcher
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-17T00:21:52Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-02-17T00:21:52Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14600714
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12608:19-905
characteristics.characteristic: Submitter Id
characteristics.text: 15
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: blood cell
characteristics.characteristic: cell_type
characteristics.text: Sham
characteristics.characteristic: clinical history
characteristics.text: pig
characteristics.characteristic: common name
characteristics.text: Protocols: Peripheral blood mononuclear cells (PBMC) were prepared from heparinised blood using Histopaque and frozen in foetal calf serum supplemented with 10% DMSO. Cells were defrosted into warm RPMI GlutaMAX HEPES media supplemented with 10% foetal calf serum, 1 mM sodium pyruvate, 50 µM 2-mercaptoethanol, 100 IU/mL penicillin and 100 µg/mL streptomycin (RPMI/10). Nucleic acid was extracted from one million cells using a MagMAX mirvRNA Total RNA extraction kit on a MagMAX™ Express-96 Deep Well Magnetic Particle Processor. 3’UTR sequencing
characteristics.characteristic: description
characteristics.text: African Swine Fever (ASF)
characteristics.characteristic: disease
characteristics.text: MHC homozygous inbred Large White Babraham
characteristics.characteristic: genotype
characteristics.text: Sus scrofa
characteristics.characteristic: organism
characteristics.text: Sus scrofa
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Non-recovered
characteristics.characteristic: phenotype
characteristics.text: Sus scrofa
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: 19-905
characteristics.characteristic: title