characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-03-30T12:17:14Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-03-30T12:17:14Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111454951
characteristics.characteristic: External Id
characteristics.text: Institute of Life Sciences, Nalco Square, Chandrasekharpur, Bhubaneswar, Odisha
characteristics.characteristic: INSDC center name
characteristics.text: 2023-03-30T12:17:14Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-03-30T12:17:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13549234
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12267:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: 36
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HL-60
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: HL-60 cells were cultured in IMDM (Iscove's Modified Dulbecco's Medium) media supplemented with 20% FBS. HL-60 wild type cells were induced with 10µM ATRA (All Trans Retinoic Acid) and 50nM Vit-D3 (Vitamin D3) for 72 hours. Leukemic cell line HL-60 was procured from ATCC and maintained in IMDM supplemented with 20% FBS. Total RNA was extracted using Qiagen RNeasy kit using manufacturer’s protocol. Quality and concentration of extracted RNA was checked using Qubit BR RNA kit and Agilent screentape respectively. Library was constructed using Trueseq stranded sample preparation kit from Illumina using Ribo-zero.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: acute promyelocytic leukemia
characteristics.characteristic: disease
characteristics.text: European
characteristics.characteristic: ethnic group
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: CD11b-negative; CD14-negative
characteristics.characteristic: immunophenotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: Sample 1
characteristics.characteristic: title