ERC000011
ENA-CHECKLIST
2023-05-01T00:15:22Z
ENA-FIRST-PUBLIC
2023-05-01T00:15:22Z
ENA-LAST-UPDATE
SAMEA110584762
External Id
Cancer Sciences, University of Southampton
INSDC center name
2023-05-01T00:15:22Z
INSDC first public
2023-05-01T00:15:22Z
INSDC last update
public
INSDC status
ERS12682448
SRA accession
E-MTAB-12055:Sample 22
Submitter Id
ArrayExpress
broker name
human
common name
Protocols: Samples were collected from patients following informed consent and in accordance with Ethics Committee approvals and the Declaration of Helsinki. Heparinised peripheral blood mononuclear cells (PBMCS) were obtained from patients attending clinic at the Southampton General Hospital. Cryopreserved samples were recovered and rested for one hour in complete RPMI-1640 (RPMI-1640 medium supplemented with 10% fetal bovine serum, 2 mM L-glutamine and 1% penicillin/streptomycin) at 37 °C before further use. Cell viability was determined by trypan blue exclusion and was ≥90% in all cases Cells were incubated with goat F(ab’)2 anti-human anti-IgM-coated or control antibody-coated DynaBeads at 37°C for 6 hours. Beads were added at a 2 beads per cell ratio Promega Reliaprep RNA Cell Miniprep System Illumina TruSeq Library Prep kit V2
description
adult
developmental stage
chronic lymphocytic leukemia
disease
816A
individual
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
anti-IgM
stimulus
Sample 22
title