ERC000011
ENA-CHECKLIST
2023-05-01T00:15:35Z
ENA-FIRST-PUBLIC
2023-05-01T00:15:35Z
ENA-LAST-UPDATE
SAMEA112688092
External Id
Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Computational Biology and Health Genomics
INSDC center name
2023-05-01T00:15:35Z
INSDC first public
2023-05-01T00:15:35Z
INSDC last update
public
INSDC status
ERS14685650
SRA accession
E-MTAB-12597:Alu blocking Control, CEBPA replicate 1
Submitter Id
8
year
age
ArrayExpress
broker name
BLaER1
cell_line
B cell
cell_type
human
common name
Protocols: For Alu blocking and CEBPA overexpression ChIP-Seq experiments, cells were collected 3h after induction of transdifferentiation. Human BLaER1 cells were grown in RPMI (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum, 2 mM L-glutamine, and 100 U/ml penicillin G sodium. 3 hours of transdifferentiation was induced in BlaER1 by addition of 100nM of β-estradiol (Calbiochem) and grown with 10nM of IL-3 and CSF-1 (Peprotech). Cells were crosslinked with 1% formaldehyde for 10 minutes at room temperature and sheared with a Qsonica sonicator. 5 μg o f chromatin and 0.5 μg of antibody against rat CEBPA (sc-61 X, Santa Cruz) were incubated in RIPA buffer (140 mM NaCl, 10 mM Tris HCl pH 8.0, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 0.1 % Na deoxycholate, protease inhibitors). DNA libraries were prepared with 1 ng of purified ChIP using NEBNext DNA Library kit for Illumina following the manufacturer’s protocol.
description
Epstein-Barr virus-related Burkitts lymphoma
disease
scrambled gRNA
genetic modification
wild type genotype
genotype
Homo sapiens
organism
Homo sapiens
organism
bone marrow
organism part
1
replicate
Homo sapiens
scientific_name
female
sex
Alu blocking Control, CEBPA replicate 1
title