ERC000011
ENA-CHECKLIST
2023-05-01T00:15:28Z
ENA-FIRST-PUBLIC
2023-05-01T00:15:28Z
ENA-LAST-UPDATE
SAMEA111539401
External Id
Genomix4lie s.r.l.
INSDC center name
2023-05-01T00:15:28Z
INSDC first public
2023-05-01T00:15:28Z
INSDC last update
public
INSDC status
ERS13649465
SRA accession
E-MTAB-12356:Control_rep1
Submitter Id
9
week
age
ArrayExpress
broker name
house mouse
common name
Protocols: Tumor specimens were macrodissected by a pathologist starting from the histological analysis of Hematoxylin-Eosin (HE)-stained slides. RNA was extracted from the macrodissected tissues by using the Qiagen RNeasy FFPE kit (Hilden, Germany) according to the manufacturer's instructions. Indexed libraries were prepared from 50ng/ea purified RNA with TruSeq RNA Exome Sample Prep Kit (Illumina) according to the manufacturer's instructions. Libraries were quantified using the TapeStation 4200 (Agilent Technologies) and Invitrogen Qubit fluorometer (Thermo Fisher Scientific), then pooled such that each index-tagged sample was present in equimolar amounts, with final concentration of the pooled samples of 2nM.
description
adult
developmental stage
colitis-associated cancer (CAC)
disease
Winnie-ApcMin/+
genotype
Mus musculus
organism
Mus musculus
organism
neoplasm
organism part
aberrant crypt foci (ACF) macrodissected from the distal colon
sampling site
Mus musculus
scientific_name
female
sex
Control_rep1
title