ERC000011
ENA-CHECKLIST
2023-04-28T00:18:50Z
ENA-FIRST-PUBLIC
2023-04-28T00:18:50Z
ENA-LAST-UPDATE
SAMEA112352807
External Id
Baker Heart and Diabetes Institute
INSDC center name
2023-04-28T00:18:50Z
INSDC first public
2023-04-28T00:18:50Z
INSDC last update
public
INSDC status
ERS14463704
SRA accession
E-MTAB-12564:Sample 10
Submitter Id
54
week
age
ArrayExpress
broker name
house mouse
common name
Protocols: tissues were collected at dissection, placed into 1.5ml tubes and immediately flash frozen in liquid nitrogen. Tissue samples were then stored at -80 degrees. RNA was extracted from ventricles and subcutaneous white adipose tissue (WAT) from each group using RNA-easy MiniPrep kit according to manufacturer’s instructions (Qiagen). RNA quality for sequencing was determined using the high-sensitivity Agilent RNA ScreenTape Assay for 4200 Tape Station System (Agilent Technologies). Only samples with an RNA Integrity Number (RIN) score >7 were selected for subsequent analysis. DNA libraries were generated as per the manufacturer’s protocol using the NEBNext Poly(A) mRNA Magnetic Isolation Module kit (New England Biolabs). 1μg total RNA per sample was inputted and adaptor ligation and PCR enrichment was performed using the NEBNext Multiplex Oligos for Illumina kit as per manufacturer’s instructions. Libraries were individually bar coded using n6-barcodes and library quantity and quality were assessed as per manufacturer’s instructions using the Agilent D1000 ScreenTape Assay for 4200 TapeStation System.
description
adult
developmental stage
normal
disease
ERalpha fl/fl
genotype
37913
individual
Mus musculus
organism
Mus musculus
organism
cardiac ventricle
organism part
Mus musculus
scientific_name
female
sex
mixed C57BL/6J and FVB/N
strain
Sample 10
title