ERC000011
ENA-CHECKLIST
2023-04-30T00:33:33Z
ENA-FIRST-PUBLIC
2023-04-30T00:33:33Z
ENA-LAST-UPDATE
SAMEA111490802
External Id
Department of Chronic Diseases, Metabolism and Ageing (CHROMETA), Translational Research Center for Gastrointestinal Disorders (TARGID), KU Leuven, Leuven, Belgium
INSDC center name
2023-04-30T00:33:33Z
INSDC first public
2023-04-30T00:33:33Z
INSDC last update
public
INSDC status
ERS13584816
SRA accession
E-MTAB-12309:GC111170
Submitter Id
ArrayExpress
broker name
human
common name
Protocols: Differentiated organoids of the AGR2 patient, UC patients or non-inflamed controls were cultured for 6 days. At day 6, Tunicamycin (1 µg/ml) or DMSO vehicle control (0.01%) was added for an additional 24 hours. Organoids were collected in lysis buffer and stored at -80 until RNA extraction. Total RNA was extracted using the RNeasy Mini Kit following the manufacturer instructions (Qiagen, Hilden, Germany). RNA was quantified by Nanodrop and the integrity was evaluated using Agilent Bio-analyzer 2100. The library construction was performed by the Lexogen QuantSeq 3’ mRNA-Seq Library Kit FWD (Lexogen, Vienna, Austria)
description
non-inflamed control
disease
organoid culture
growth condition
Control 10
individual
Homo sapiens
organism
Homo sapiens
organism
colon
organism part
Homo sapiens
scientific_name
GC111170
title