ERC000011
ENA-CHECKLIST
2023-04-29T00:15:48Z
ENA-FIRST-PUBLIC
2023-04-29T00:15:48Z
ENA-LAST-UPDATE
SAMEA110397360
External Id
MRC Human Genetics Unit, Institute of Genetics and Cancer, University of Edinburgh
INSDC center name
2023-04-29T00:15:48Z
INSDC first public
2023-04-29T00:15:48Z
INSDC last update
public
INSDC status
ERS12495555
SRA accession
E-MTAB-11959:do17798
Submitter Id
15
day
age
ArrayExpress
broker name
house mouse
common name
Protocols: Livers from P15 male C3H/HeOuJ mice were perfused in situ with PBS and then dissected, minced, cross-linked using 1% formaldehyde solution for 20 min, quenched for 10 min with 250 mM glycine, washed twice with ice-cold PBS, and tissue pellets were stored at -80°C. Tissues were homogenised, washed twice with PBS, and lysed according to published protocols (Schmidt et al. 2009). Ten samples were pooled, and chromatin was sonicated to an average fragment length of 300 bp. Chromatin was immunoprecipitated using 20 μg CTCF antibody (rabbit polyclonal, Merck Millipore 07-729, lot 2517762). Immunoprecipitated DNA or input DNA (max 50 ng) was used for library preparation using the ThruPLEX DNA-Seq library preparation protocol (Rubicon Genomics, UK) as described previously (Aitken et al. 2018). Library fragment size was determined using a 2100 Bioanalyzer (Agilent).
description
P15
developmental stage
pool of 10 individuals B
individual
Mus musculus
organism
Mus musculus
organism
liver
organism part
Mus musculus
scientific_name
male
sex
C3H/HeJ
strain
do17798
title