ERC000011
ENA-CHECKLIST
2023-05-01T00:15:37Z
ENA-FIRST-PUBLIC
2023-05-01T00:15:37Z
ENA-LAST-UPDATE
SAMEA112857496
External Id
UCD School of Medicine and UCD Conway Institute, University College Dublin, Dublin, Ireland
INSDC center name
2023-05-01T00:15:37Z
INSDC first public
2023-05-01T00:15:37Z
INSDC last update
public
INSDC status
ERS14864156
SRA accession
E-MTAB-12792:Veh_IN_13
Submitter Id
ArrayExpress
broker name
SUIT2-007
cell_line
human
common name
Protocols: ChIP-seq was performed on Suit2 cells treated for 72 h with either DMSO (vehicle control), RAR-β agonist (CD 2314, Tocris 3824), or RAR-β antagonist (LE 135, Tocris). Suit2-007 cells (PDAC cell line) were cultured in Dulbecco’s Modified Eagle’s Media (Sigma-Aldrich, D8437) supplemented with 10% v/v FBS (Sigma Aldrich, F7524), 2 mM L-glutamine (Sigma Aldrich, G7513), 1% v/v penicillin/streptomycin (Sigma-Aldrich, P4333) and 1 % v/v fungizone Amphotericin B (Gibco, UK, 15290-026). Cells were incubated at 37°C, with 5% CO2. ChIP-seq was performed on Suit2 cells treated for 72 h with either DMSO (vehicle control), 1 µM RAR-β agonist (CD 2314, Tocirs 3824), or 1 µM RAR-β antagonist (LE 135, Tocris 2021). Chromatin was immunoprecipitated using the SimpleChIP Plus Sonication Chromatin IP kit (Cell Signaling Technology, 56383) according to manufacturer’s instructions using a rabbit polyclonal anti RAR-β antibody (Abcam ab53161) at 2 µg sample. Rabbit monoclonal anti-histone H3 (clone D2B12, Cell Signaling Technology, 4620) was used as a positive control. ChIP-seq libraries were prepared from NEBNext Ultra II DNA library Prep Kit (Illumina)
description
adult
developmental stage
pancreatic ductal adenocarcinoma
disease
wild type genotype
genotype
liver
metastatic site
Homo sapiens
organism
Homo sapiens
organism
pancreatic duct
organism part
Homo sapiens
scientific_name
male
sex
Veh_IN_13
title