ERC000011
ENA-CHECKLIST
2023-05-01T00:15:36Z
ENA-FIRST-PUBLIC
2023-05-01T00:15:36Z
ENA-LAST-UPDATE
SAMEA112819548
External Id
LECA, Laboratoire d'Ecologie Alpine, CNRS- Universite Grenoble Alpes UMR 5553 CS 40700 38058 Grenoble cedex 9, France
INSDC center name
2023-05-01T00:15:36Z
INSDC first public
2023-05-01T00:15:36Z
INSDC last update
public
INSDC status
ERS14827084
SRA accession
E-MTAB-12756:Herb_Ins4
Submitter Id
3
day
age
ArrayExpress
broker name
African malaria mosquito
common name
Protocols: For each replicate, 30 three-day-old non-blood fed female An. gambiae, collected and stored in RNA-later at -20ºC. Total RNA was extracted from each pool using Trizol (Life Technologies) according to manufacturer's instructions, and treated with DNase to remove genomic DNA contaminants. RNA-seq libraries were prepared from 150 ng total RNA using NEBNext Ultra II Directional RNA library Prep Kit for Illumina (New England Biolabs) following manufacturer's instructions. Libraries were quantified using the Qubit DNA BR assay (Thermofisher Scientific) and quality checked using Bioanalyzer DNA 1000 assay (Agilent).
description
adult
developmental stage
non blood fed
diet
wild type genotype
genotype
mixed insecticide and non-insecticide cocktail
growth condition
pool of 30 individuals
individual
Anopheles gambiae
organism
Anopheles gambiae
organism
whole organism
organism part
Anopheles gambiae
scientific_name
female
sex
Tiassale_S
strain
Herb_Ins4
title