ERC000011
ENA-CHECKLIST
2023-05-01T00:15:31Z
ENA-FIRST-PUBLIC
2023-05-01T00:15:31Z
ENA-LAST-UPDATE
SAMEA112224461
External Id
Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University in Vestec (BIOCEV); First Faculty of Medicine; Charles University
INSDC center name
2023-05-01T00:15:31Z
INSDC first public
2023-05-01T00:15:31Z
INSDC last update
public
INSDC status
ERS14335303
SRA accession
E-MTAB-12446:Rps19R67∆/R67∆ Trp53−/− A
Submitter Id
10
week
age
ArrayExpress
broker name
hematopoietic multipotent progenitor cell
cell_type
house mouse
common name
Protocols: Bone marrow cells from 10-week WT control, homozygous Rps19R67∆, Trp53−/− mutant, and double-homozygous Rps19R67∆/R67∆Trp53−/− males were collected into ice cold DMEM with 20% FBS and stained with anti-CD117-BV421, anti-Sca1-PE, and anti-lineage (Ter119, Gr1, Mac1, B220, Cd3e) antibody cocktail. Bone marrows were enriched for lineage-negative Sca1−c-Kit+ (LS−K) and Lin−Sca1+c-Kit+ (LSK) stem cell populations by fluorescence-activated cell sorting using BD Aria II SORP cell sorter. Cell suspensions were concentrated to approximate cell density of 1000 cells/uL. Following enrichment by fluorescence-activated cell sorting the viability of hematopoietic progenitors in cell suspension was assessed. Only cell suspensions with viability > 90% were processed using Chromium Next GEM Single Cell 3ʹ Kit v3.1 (dual index) library preparation kit (10x Genomics, CA, USA). Libraries were prepared by loading approximately 20000 cells per well of Chromium Next GEM Single Cell 3ʹ Kit v3.1 (dual index) library preparation kit (10x Genomics, CA, USA) and libraries were prepared by following manufacturer's protocol.
description
adult
developmental stage
Diamond-Blackfan anemia
disease
Rps19R67∆/R67∆ Trp53−/−
genotype
RPS19/TRP53 1
individual
Mus musculus
organism
Mus musculus
organism
bone marrow
organism part
Mus musculus
scientific_name
male
sex
C57BL/6J
strain
Rps19R67∆/R67∆ Trp53−/− A
title