characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-09T05:00:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-09T05:00:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112183587
characteristics.characteristic: External Id
characteristics.text: Sun Yat-sun University Zhongshan Ophathlamic Center
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-09T05:00:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-09T05:00:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14293479
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12399:Sample 3
characteristics.characteristic: Submitter Id
characteristics.text: 5
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: crab-eating macaque
characteristics.characteristic: common name
characteristics.text: Protocols: Total 6 monkeys (4-15 years old, Macaca fascicularis) were used in the current project. All procedures complied with the NIH Guide for the Care and Use of Laboratory Animals approved by Sun Yat-sen University. Following 2 weeks of adaptive housing in the institutional animal facility, animals were anesthetized with a lethal dose of sodium pentobarbital. Animals were transcardially perfused with 2 liters of artificial cerebrospinal fluid. The flow of perfusion fluid was adjusted so that the perfusion finished within approximately 20 minutes. The brain was removed from the skull immediately and a block of tissue containing the hippocampus was cut off. The position of hippocampus referred to the atlas of the rhesus monkey brain (Saleem and Logothetis, 2012). The tissue block was kept in the ACSF and sectioned on a vibratome (VT1200S, Leica) in 300 um thick slices. The adherent tissues around the hippocampus of each slice were removed on ice and only the regions of interest were kept for dissociation. 10X single cell isolation protocol 10X Genomics Chromium platform
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: individual 3
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Macaca fascicularis
characteristics.characteristic: organism
characteristics.text: Macaca fascicularis
characteristics.characteristic: organism
characteristics.text: prefrontal cortex
characteristics.characteristic: organism part
characteristics.text: Macaca fascicularis
characteristics.characteristic: scientific_name
characteristics.text: Sample 3
characteristics.characteristic: title