characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-01T00:15:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-01T00:15:25Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111468766
characteristics.characteristic: External Id
characteristics.text: Leonard Davis School of Gerontology University of Southern California
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-01T00:15:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-01T00:15:25Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13562796
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12289:N2-4
characteristics.characteristic: Submitter Id
characteristics.text: 1
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: For RNA isolation, all RNa collection was performed at day 1 of adulthood. ~1000 animals were harvested from RNAi plates using M9. Animals were pelleted by gravity by allowing adult worms to settle to the bottom of the tube and aspirating off eggs and L1. Animals were washed and gravity settled 3x to remove a majority of progeny, then animals were placed into Trizol solution and worms were freeze/thawed 3x with liquid nitrogen with a 30 sec vortexing step between each freeze cycle. Standard bleaching protocol was used to synchronize animal populations. Animals were L1 arrested for a maximum of 16 hours to further synchronize populations. Animals were grown on RNAi indicated from L1 until Day 1 of adulthood and material was collected on Day 1. After the final thaw, chloroform was added at a 1:5 chlorform/trizol ratio and aqueous separation of RNA was performed via centrifugation in a heavy gel phase-lock tube (VWR, 10847-802). The aqueous phase was mixed 1:1 with isopropanol then applied to a Qiagen RNeasy Mini Kit (74106) and RNA purification was performed as per manufacturer’s directions. Library preparation was performed using a Kapa Biosystems mRNA Hyper Prep Kit.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: organism
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: scientific_name
characteristics.text: hermaphrodite
characteristics.characteristic: sex
characteristics.text: N2
characteristics.characteristic: strain
characteristics.text: N2-4
characteristics.characteristic: title