characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-10T00:18:13Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-10T00:18:13Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110177414
characteristics.characteristic: External Id
characteristics.text: MD Anderson
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-10T00:18:13Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-10T00:18:13Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12276220
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11870:MJ20_FL
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HEK293T
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: 293T cells were transduced with empty vector, plasmid expressing RUNX1 FL or RUNX1 delEx4 isoform. The plasmids are all doxycycline inducible. The cells were induced with doxyclycline (0.4 mg/ml) for 72 hours following which RNA was collected and sequenced. RNA was extracted using Zymo Quick-RNA columns (Zymo Research, Irvine, CA). Library construction was performed using the NEB library kit for non-directional libraries as per the manufacturer's instructions
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: RUNX1 full length overexpression
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: kidney
characteristics.characteristic: organism part
characteristics.text: RUNX1 overexpression
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: MJ20_FL
characteristics.characteristic: title