characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-05-14T00:15:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-05-14T00:15:01Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110644500
characteristics.characteristic: External Id
characteristics.text: EMBL
characteristics.characteristic: INSDC center name
characteristics.text: 2023-05-14T00:15:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-05-14T00:15:01Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12742265
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12100:21-032_2_ctrl_H3K27me3_rep1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: mESC
characteristics.characteristic: cell_line
characteristics.text: embryonic stem cell
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Naive murine embryonic stem cells (mESC) were derived freshly (FVB or mixed 129/B6) and were routinely cultured on gelatin-coated plates in t2i/L media: NDiff (N2B27) (Takara #y40002) supplemented with titrated 2i (0.2__M PD0325901 and 3__M CHIR 99021), 1,000_U/ml leukaemia inhibitory factor (LIF), 1% FBS, 1% penicillin streptomycin and maintained in humidified atmosphere at 37oC and 5% CO2. Cells were passaged every 2_days by dissociation with TrypLE and medium was changed daily. This protocol has been optimised from Skene and Henikoff, Elife, 2017 to detect interaction between DNA and histone modifications. Briefly, fresh cells are harvested, permeabilised, bound to concanavallin magnetic beads and incubated with the antibody for the modification of interest. Samples were then incubated with pA-MNase to cut the DNA side of the bound antibody and the DNA fragments were eluted and subjected to size selection. DNA library was generated with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S).
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: FVB
characteristics.characteristic: strain
characteristics.text: 21-032_2_ctrl_H3K27me3_rep1
characteristics.characteristic: title