characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-05-22T12:00:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-05-22T12:00:08Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112483910
characteristics.characteristic: External Id
characteristics.text: University of Galway
characteristics.characteristic: INSDC center name
characteristics.text: 2023-05-22T12:00:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-05-22T12:00:08Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14601260
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12578:PTBP1_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: MCF 10A
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were harvested, centrifuged and stored at -80oC Cells lines were grown in DMEM supplemented with 5% horse serum, 25 ng/ml cholera toxin, 10 μg/ml insulin, 20 ng/ml epidermal growth factor (Peprotech), 500 ng/ml hydrocortisone, 50 U/ml penicillin and 50 μg/ml streptomycin. Total RNA was isolated from MCF10A EditR and MCF10A PTBP1 mutant clone 4.1 cells using the RNeasy Plus Micro kit, according to manufacturer instruction. The quality and concentration of RNA were determined on a 2100 Bioanalyzer using the Agilent RNA 6000 Nano assay. Novogene
characteristics.characteristic: description
characteristics.text: breast cancer
characteristics.characteristic: disease
characteristics.text: CrispR/Cas9 knockout of PTBP1
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: mammary gland
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: PTBP1_2
characteristics.characteristic: title