characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-09T12:23:32Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-09T12:23:32Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110460944
characteristics.characteristic: External Id
characteristics.text: Department of General, Visceral and Pediatric Surgery, University Medical Center Gottingen
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-09T12:23:32Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-09T12:23:32Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12558972
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12022:PT44 Tumor
characteristics.characteristic: Submitter Id
characteristics.text: 69
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: replacement histopathological growth pattern
characteristics.characteristic: clinical information
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: CRCLM and normal, non-transformed hepatic tissue located as distant to the metastases as possible from 6 patients with CRCLM (3 dHGP vs 3 rHGP) (Supplementary Table 1) was used for scRNA-seq. The HGP was determined retrospectively in H&E slides covering the tumor-liver interface at the margin of sample collection. Specimens underwent single-cell dissociation with the gentleMACs Octo Dissociator (Miltenyi Biotec,130-095-937), cryopreservation at -80°C and magnetic-activated cell sorting (MACS) with Basic MicroBeads (Miltenyi Biotec, 130-048-001) for depletion of dead cells. Nucleic acid extraction was performed using the 10xGenomics Chromium Next GEMSingle Cell 3ʹ Reagent Kits v3.1 following the given protocol (CG000204) Nucleic acid library construction was performed using the 10xGenomics Chromium Next GEMSingle Cell 3ʹ Reagent Kits v3.1 following the given protocol (CG000204 Rev D)
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: metastatic colorectal cancer
characteristics.characteristic: disease
characteristics.text: PT44
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: neoplasm
characteristics.characteristic: sampling site
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: PT44 Tumor
characteristics.characteristic: title