characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-05-15T15:00:52Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-05-15T15:00:52Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112642591
characteristics.characteristic: External Id
characteristics.text: Department of rheumatology Tokyo Medical and Dental University (TMDU)
characteristics.characteristic: INSDC center name
characteristics.text: 2023-05-15T15:00:52Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-05-15T15:00:52Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14634560
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12618:ob_Lung_non3
characteristics.characteristic: Submitter Id
characteristics.text: 19
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mouse-adapted SARS-CoV-2 strain was established in the previous study. Mice were anesthetized and then inoculated viral solution intranasally (i.n.). Viral solutions for inoculation were diluted with PBS and 30 µl of solution contained twice of the 50% lethal dose (2LD50) determined in 24-week-old BALB/c mice. At three post infection, mice were anesthetized by intraperitoneal injection with a mixture of 0.75 mg/kg of medetomidine, 4.0 mg/kg of midazolam, and 5.0 mg/kg of butorphanol. Lung samples were dissected from mice and immediately immersed in RNA later (Invitrogen) stabilization reagent and stored at -80℃ for 24 hr. Samples were transferred to a new tube containing 1-mm glass beads and 700μL Trizol (Invitrogen). Tissues were subsequently homogenized using a MINI BEAD-BEATER (Biospec). Total RNA was extracted and purified using a RNeasy mini kit (QIAGEN, Tokyo, Japan) and quantified using a spectrophotometer (NanoDrop one, Thermo Fisher Scientific). Total RNA was extracted from the lung as described above and checked for quality using RNA integrity scores were typically 7.0 and greater with an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA). For the quality control, mRNA had to meet the requirement of 28S/18S > 1, while miRNA had to meet the requirement of 28S/18S > 1.5. According to the manufacturer's protocol, the RNA was fragmented into small pieces. Afterward, the fragmented RNA was reverse-transcribed into cDNA and amplified with polymerase chain reaction (PCR) to create a cDNA library. Quality control and quantification of the libraries were performed with the Agilent 2100 bioanalyzer and real-time quantitative PCR (qPCR) (TaqMan Probe).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: leptin deficient
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: lung
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6JHamSlc-ob/ob
characteristics.characteristic: strain
characteristics.text: ob_Lung_non3
characteristics.characteristic: title