characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-05-30T11:00:04Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-05-30T11:00:04Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111350655
characteristics.characteristic: External Id
characteristics.text: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium  VIB Center for Plant Systems Biology, VIB, Gent, Belgium
characteristics.characteristic: INSDC center name
characteristics.text: 2023-05-30T11:00:04Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-05-30T11:00:04Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13448202
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12174:4DAE_R2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: After around 35 days of growth all stage 12c flowers on the main stem were emasculated and harvested after 2, 3, or 4 days. Unpollinated pistils of emasculated wild-type flowers were dissected as described previously. For each time point the ovules of over 75 pistils (approximately 4000 ovules) were collected on dry ice with the help of a vacuum-driven micro-aspirator (Sanchez-Leon et al., 2012). The whole time series was sampled in three independent batches (four time points in each round, 2 to 4 DAE). Arabidopsis seeds (Columbia-0, Col-0) were sown on Jiffy-7C propagation plugs (Jiffy) and stratified for two to three days at 4 °C. Plants were grown for about 35 days at 22°C under a 16 h light/8 h dark regime before being emasculated. After subsequent tissue grinding, RNA was extracted with the Spectrum Plant Total RNA kit (Sigma). RNA quantity and quality was assessed by spectrophotometric analysis (NanoDrop ND-2000, Thermo) and on-chip electrophoresis (RNA 6000 Nano Kit, Agilent). Library preparation was done using the TruSeq unstranded RNA Sample Preparation Kit v2 (Illumina).
characteristics.characteristic: description
characteristics.text: mature non-fertilized ovule
characteristics.characteristic: developmental stage
characteristics.text: Col-0
characteristics.characteristic: ecotype
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: plant ovule
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: 4DAE_R2
characteristics.characteristic: title