characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-05-31T00:15:38Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-05-31T00:15:38Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112127980
characteristics.characteristic: External Id
characteristics.text: Department of Biochemistry, University of Otago
characteristics.characteristic: INSDC center name
characteristics.text: 2023-05-31T00:15:38Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-05-31T00:15:38Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14237012
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12370:WT_rep2
characteristics.characteristic: Submitter Id
characteristics.text: 12
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Columbia
characteristics.characteristic: cultivar
characteristics.text: Protocols: Three biological replicates were collected. Whole seedlings were harvested. Wild-type, lsm4-1, LSM4R and LSM4RxK in lsm4-1 background seedlings were grown on Murashige and Skoog (MS) medium containing 0.8% (w/v) agar for 12 days under continuous light at 22°C. Whole seedlings were harvested and total RNA was extracted with RNeasy Plant Mini Kit (QIAGEN) following the manufacturer’s protocols. To estimate RNA concentration NanoDrop 2000c (Thermo Scientific) was used. Libraries were prepared by genome center at the Max Planck-Genome-Centre Cologne (MP-GC).
characteristics.characteristic: description
characteristics.text: seedling development stage
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: 2
characteristics.characteristic: replicate
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: WT_rep2
characteristics.characteristic: title