characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-05-27T00:18:42Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-05-27T00:18:42Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112795192
characteristics.characteristic: External Id
characteristics.text: Biosante UMR 1292 Health Department (DS) Interdisciplinary Research Institute of Grenoble (IRIG)
characteristics.characteristic: INSDC center name
characteristics.text: 2023-05-27T00:18:42Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-05-27T00:18:42Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14795977
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12776:CX4945
characteristics.characteristic: Submitter Id
characteristics.text: 58
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: 786-O
characteristics.characteristic: cell_line
characteristics.text: epithelial cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: 786-O cells were grown in RPMI medium containting 10% FCS for 24h before treatment. 786-O cells (150, 000) were treated for 24h with 0.1% DMSO or 5 µM of CX4945 or AB668. ccRCC cell lines 786-O was obtained from ATCC. Total RNA was extracted from treated cells using the MirVana PARIS kit (Thermofisher). The 3′ Bulk RNA Barcoding and sequencing (BRB-seq) experiments were performed at the Research Institute for Environmental and Occupational Health (Irset, Rennes, France) according to the published protocol (Alpern et al., 2019).The sequencing libraries were built by tagmentation using 50 ng of ds cDNA with Illumina Nextera XT Kit (Illumina, #FC-131-1024)
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: renal cell carcinoma
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: CX4945
characteristics.characteristic: title