characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-05-05T00:15:11Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-05-05T00:15:11Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112156554
characteristics.characteristic: External Id
characteristics.text: HENAN AGRICULTURAL UNIVERSITY
characteristics.characteristic: INSDC center name
characteristics.text: 2023-05-05T00:15:11Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-05-05T00:15:11Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14266451
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12363:PEK-1, Peking roots replicate 1
characteristics.characteristic: Submitter Id
characteristics.text: 2
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: soybean
characteristics.characteristic: common name
characteristics.text: Peking
characteristics.characteristic: cultivar
characteristics.text: Protocols: The soybean seeds were pre-germinated on moistened filter paper in a plant growth chamber at 27 °C, 85% ambient humidity and a 16:8 (light: dark) photoperiod for 3-4 days. The seedlings were then transferred into tubes (5 cm 3 30 cm) with sandy soil at 27°C under 16 h of light in the greenhouse. When the plants had three fully expanded trifoliate leaves, roots were harvested, flash frozen in liquid nitrogen, ground to powder, and stored at -80 °C until the next step. For each sample, three biological replicates were set up. The soybean seeds were pre-germinated on moistened filter paper in a plant growth chamber at 27 °C, 85% ambient humidity and a 16:8 (light: dark) photoperiod for 3-4 days. The seedlings were then transferred into tubes (5 cm 3 30 cm) with sandy soil at 27°C under 16 h of light in the greenhouse. The root samples were ground into two parts, one of which was used to extract total RNAs using TRIZOL reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA concentration and purity were assayed with a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). The RNA integrity was assessed on an Agilent 2100 Bioanalyzer Lab-on-Chip system (Agilent Technologies, Palo Alto, CA, USA). The construction of cDNA libraries was according to the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA). The clustering of samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia, San Diego, USA) according to the manufacturer’s instructions.
characteristics.characteristic: description
characteristics.text: V3 stage
characteristics.characteristic: developmental stage
characteristics.text: pool of six roots
characteristics.characteristic: individual
characteristics.text: Glycine max
characteristics.characteristic: organism
characteristics.text: Glycine max
characteristics.characteristic: organism
characteristics.text: root
characteristics.characteristic: organism part
characteristics.text: 1
characteristics.characteristic: replicate
characteristics.text: Glycine max
characteristics.characteristic: scientific_name
characteristics.text: PEK-1, Peking roots replicate 1
characteristics.characteristic: title