characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-09T00:24:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-09T00:24:08Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110379025
characteristics.characteristic: External Id
characteristics.text: Institut de Genetique et de Biologie Moleculaire et Cellulaire, Illkirch, France Centre National de la Recherche Scientifique, UMR7104, Illkirch, France Institut National de la Sante et de la Recherche Medicale, U1258, Illkirch, France Universite de Strasbourg, Illkirch, France
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-09T00:24:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-09T00:24:08Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12477221
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11970:Sample 2
characteristics.characteristic: Submitter Id
characteristics.text: 108 to 117 hours after egg laying
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: hemocyte
characteristics.characteristic: cell_type
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: For each replicate, the hemocytes were bled from 100 wandering 3rd instar larvae of the following genotypes: (1) gcm26/+ issued from the cross gcm26/CyOGFP x w1118, (2) Toll10b/+ issued from the cross Toll10b/Ser,TM3 x w1118 and (3) gcm26/+;Toll10b/+ issued from the cross gcm26/CyOGFP x Toll10b/Ser,TM3. The RNA were extracted using Tri reagent (Sigma) following the manufacturer’s instruction. Library preparation and sequencing were carried out by the genomeast facility (https://www.igbmc.fr/plateformes-technologiques/genomeast) at the IGBMC. Stranded mRNA libraries were prepared with Directional mRNA-Seq (SamplePrep).
characteristics.characteristic: description
characteristics.text: wandering third instar larval stage
characteristics.characteristic: developmental stage
characteristics.text: gcm26/+
characteristics.characteristic: genotype
characteristics.text: pool of 100 larvae
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: hematopoietic system
characteristics.characteristic: organism part
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: mixed
characteristics.characteristic: sex
characteristics.text: w1118
characteristics.characteristic: strain
characteristics.text: Sample 2
characteristics.characteristic: title