characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-05-22T10:00:07Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-05-22T10:00:07Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110584743
characteristics.characteristic: External Id
characteristics.text: Onderzoeksgroep Nefrologie en Niertransplantatie
characteristics.characteristic: INSDC center name
characteristics.text: 2023-05-22T10:00:07Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-05-22T10:00:07Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12682430
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12051:Sample 13
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: kidney transplant
characteristics.characteristic: clinical history
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Kidney biopsy samples were collected at indicated time at Leuven' university hospital, Belgium. Cells were isolated after mechanical and enzymatic dissociation and resuspended in PBS containing 0.04% UltraPure BSA (AM2616, ThermoFisher Scientific) and strained through a 40 µm cell strainer to further remove cell clumps and large fragments. Cell number and viability was measured using Luna Cell counter. Libraries for scRNASeq were generated using the Chromium Single Cell 5′ library and Gel Bead & Multiplex Kit from 10x Genomics. We aimed to profile 2000 cells per library if sufficient cells were retained during the process. All libraries were sequenced on Illumina NextSeq until sufficient saturation was reached.
characteristics.characteristic: description
characteristics.text: Non rejection DSA+
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: kidney
characteristics.characteristic: organism part
characteristics.text: kidney transplant biopsy
characteristics.characteristic: sampling site
characteristics.text: 118 days after transplantation
characteristics.characteristic: sampling time point
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: Sample 13
characteristics.characteristic: title