characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-01T00:15:37Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-01T00:15:37Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112770671
characteristics.characteristic: External Id
characteristics.text: Microbiome and Antitumor Immunity Dpt. of Experimental Oncology European Institute of Oncology, Milan, Italy
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-01T00:15:37Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-01T00:15:37Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14771477
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12645:Sample 6
characteristics.characteristic: Submitter Id
characteristics.text: 2
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: mononuclear cell
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Spleen and lymph nodes (inguinal and axillary) were harvested from mice under sterile conditions, mechanically dissociated into single cell suspension and red blood cells were lysed. Cells were then sorted by negative selection following the manufacturer’s instructions. After sorting, cells were resuspended at 1x106 cells/mL in complete RPMI media (10% FBS, 1% NaPy, 1% Glutamine, 1% Pen/Strep, 0,1% b-mercaptoethanol) supplemented with 100U/mL IL2. For OT1 mice, media was further complemented with 2 mg/mL of SIINFELK peptide, while for C57J/B6 mice polyclonal activation was achieved using 5 mg/mL plated-bound aCD3 and 0,5 mg/mL soluble aCD28. Cells were further treated with BSA, linoleic acid, oleic acid or arachidonic acid. OT1 cells were sorted with >95% purity as previously described, activated for 48 hours in the presence or absence of LA, counted and resuspended in RLT lysis buffer (Qiagen). RNA was isolated from purified cells using RNeasy Mini kits, and RNA concentrations were determined using Nanodrop. RNA-seq, Random sequencing of whole transcriptome.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: mixed spleen and lymph node
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: 5 mg/mL plated-bound aCD3 and 0,5 mg/mL soluble aCD28
characteristics.characteristic: stimulus
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: Sample 6
characteristics.characteristic: title