characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-05-31T00:15:39Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-05-31T00:15:39Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112228593
characteristics.characteristic: External Id
characteristics.text: Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University in Vestec (BIOCEV); First Faculty of Medicine; Charles University, Czech Republic
characteristics.characteristic: INSDC center name
characteristics.text: 2023-05-31T00:15:39Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-05-31T00:15:39Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14339493
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12448:Rps19R67∆/R67∆ 1
characteristics.characteristic: Submitter Id
characteristics.text: embryonic day 14.5
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Fetal liver from embryos from timed crossings of mating pairs of appropriate genotypes were harvested 14 days after detection of plugs (E14.5). Pregnant females were euthanized and they embryos extracted in ice-cold PBS. Fetal livers were collected in PBS and dissolved by pipetting and small aliquot was used for preparation of RNA in Trizol reagent within 1 hour after extraction of mouse embryos. Total RNA from freshly harvested fetal livers was isolated with Trizol reagent following manufacturer's protocol. 1µg of DNase I-treated RNA isolated from treated cells was used for poly-A mRNA isolation with the NEBNext Poly(A) mRNA Magnetic Isolation Module. Paired-end libraries were prepared using the NEBNext Ultra II Directional RNA Library Kit with NEBNext multiplex index oligos for Illumina (Dual Index Primers Set 1) (New England Biolabs).
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: Diamond-Blackfan anemia
characteristics.characteristic: disease
characteristics.text: Rps19R67∆/R67∆
characteristics.characteristic: genotype
characteristics.text: Q4954-E2
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: Rps19R67∆/R67∆ 1
characteristics.characteristic: title