characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-10T00:18:14Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-10T00:18:14Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110190668
characteristics.characteristic: External Id
characteristics.text: MD Anderson
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-10T00:18:14Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-10T00:18:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12288346
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11886:HiG1014
characteristics.characteristic: Submitter Id
characteristics.text: 13.5 to 16.5
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: fetal liver hematopoietic progenitor cell
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Fetal liver cells (FLCs) were isolated from pregnant wildtype CD45.2+ C57Bl/6 females between days 13.5 – 16.5 of gestation. The FLCs were transduced with either empty GFP vector or hnRNP K GFP plasmid. The transduced cells were then sorted for GFP positivity using flow cytometry. RNA was extracted and purified from GFP+ sorted FLCs using Zymo Quick-RNA columns (Zymo Research, Irvine, CA). Barcoded, Illumina-stranded total RNA libraries were prepared using the TruSeq Stranded Total RNA Sample Preparation Kit (Illumina, San Diego, CA). Briefly, 250 ng of DNase I-treated total RNA was depleted of cytoplasmic and mitochondrial ribosomal RNA (rRNA) using Ribo-Zero Gold (Illumina, San Diego, CA). After purification, RNA was fragmented using divalent cations and double stranded cDNA was synthesized using random primers. The ends of the resulting double stranded cDNA fragments were repaired, 5′-phosphorylated, 3’-A tailed and Illumina-specific indexed adapters are were ligated. The products were purified and enriched by 12 cycles of PCR to create the final cDNA library. The libraries were quantified by qPCR and assessed for size distribution using the 4200 TapeStation High Sensitivity D1000 ScreenTape (Agilent Technologies, Santa Clara, CA).
characteristics.characteristic: description
characteristics.text: embryo stage
characteristics.characteristic: developmental stage
characteristics.text: hnRNP K GFP overexpression
characteristics.characteristic: genotype
characteristics.text: GFP+
characteristics.characteristic: immunophenotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: HiG1014
characteristics.characteristic: title