characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-01T00:15:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-01T00:15:25Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111468769
characteristics.characteristic: External Id
characteristics.text: Faculty of Biology, Medicine and Health, University of Manchester
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-01T00:15:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-01T00:15:25Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13562799
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12292:TRIM28 rep1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: U2OS
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: U2OS cells were grown in DMEM medium containing 10% foetal bovine serum at 37°C incubator with 5% CO2. After reverse cross-link and protein digestion with Proteinase K (Roche), DNA was purified using QIAquick PCR purification kit (Qiagen) following the manufacturer's instructions. ChIP-seq was performed as described in Schmidt et al., 2009 with modifications, using 5 x 10^7 U2OS. Immuno-precipitation was performed overnight at 4°C by incubating the shared DNA-protein complex with 10 µg of anti-ADNP or anti-TRIM28 antibody, followed by adding Dynabeads protein A (Invitrogen) and incubating for further 2 hours. Schmidt D, Wilson MD, Spyrou C, Brown GD, Hadfield J, Odom DT. (2009) ChIP-seq: using high-throughput sequencing to discover protein-DNA interactions. Methods 48: 240-248.
characteristics.characteristic: description
characteristics.text: juvenile stage
characteristics.characteristic: developmental stage
characteristics.text: osteosarcoma
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: tibia
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: TRIM28 rep1
characteristics.characteristic: title