characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-05-15T00:14:48Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-05-15T00:14:48Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112994109
characteristics.characteristic: External Id
characteristics.text: EMBL
characteristics.characteristic: INSDC center name
characteristics.text: 2023-05-15T00:14:48Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-05-15T00:14:48Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14989133
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12957:i5TFs dox V+CD+_2b
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: i5TFs mESC
characteristics.characteristic: cell_line
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Doxycycline-inducible ESC lines were generated using the inducible cassette exchange method, the constructs and plasmids described in Bergiers et al., 2018; Iacovino et al., 2011; Vargel et al., 2016. Through gene synthesis (GenScript) and cloning, p2lox-5TFs and p2lox-3TFs plasmids were generated. They were consequently transfected into A2.lox ESCs (kindly provided by Dr. Michael Kyba - University of Minnesota). ESCs were maintained, expanded and differentiated as described in Serina Secanechia et al., 2022 Cell sorting, culture and transcription factor (TF) overexpression were performed as described in Serina Secanechia et al., 2022 RNA-seq library preparation and sequencing were performed as described in Picelli et al., 2014 and Serina Secanechia et al., 2022
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: gene knock in
characteristics.characteristic: genetic modification
characteristics.text: knock in of hematopoietic genes Runx1, Cbfb, Gata2, Fli1, Erg under a dox-inducible promoter
characteristics.characteristic: genotype
characteristics.text: VE-CADHERIN+CD41+
characteristics.characteristic: immunophenotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: doxycycline-induced expression of Runx1, Cbfb, Gata2, Fli1, Erg
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: doxycycline (10µg/mL)
characteristics.characteristic: stimulus
characteristics.text: i5TFs dox V+CD+_2b
characteristics.characteristic: title