characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-12T17:00:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-12T17:00:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112652323
characteristics.characteristic: External Id
characteristics.text: Center for synthetic microbiology SYNMIKRO Philipps University Marburg
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-12T17:00:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-12T17:00:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14650034
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12644:NAD+ resistent clone 3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Streptococcus pneumoniae was cultivated in liquid medium with increasing concentrations (50 µM to 5 mM) of NAD+. After six passages, bacteria were plated on blood agar supplemented with 500 µM NAD+ and three clones were picked Total DNA for resequencing was extracted using a DNeasy Blood and Tissue kit (Qiagen) according to the manual. DNA libraries for sequencing were generated by applying a Nextera XT DNA Library Preparation kit (Illumina)
characteristics.characteristic: description
characteristics.text: spontaneus NAD+ resistent clone
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Streptococcus pneumoniae
characteristics.characteristic: organism
characteristics.text: Streptococcus pneumoniae
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Streptococcus pneumoniae
characteristics.characteristic: scientific_name
characteristics.text: D39
characteristics.characteristic: strain
characteristics.text: NAD+ resistent clone 3
characteristics.characteristic: title