characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-05-31T00:15:39Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-05-31T00:15:39Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112227666
characteristics.characteristic: External Id
characteristics.text: Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University in Vestec (BIOCEV); First Faculty of Medicine; Charles University, Czech Republic
characteristics.characteristic: INSDC center name
characteristics.text: 2023-05-31T00:15:39Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-05-31T00:15:39Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14338571
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12447:Wild-type 1
characteristics.characteristic: Submitter Id
characteristics.text: 8
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: hematopoietic multipotent progenitor cell
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: A single cell suspension of bone marrow from 6 to 8-week-old mice were obtained by flushing tibias and femurs with 1 ml ice-cold Flow Cytometry Buffer (FCB, 1x PBS with 2mM EDTA and 0.5+ACU- biotin-free BSA) using 22-gauge needles. Bone marrow hematopoietic c-kit progenitors were enriched by BD Aria II SORP cell sorter using fluorescence-activated cell sorting with anti-CD117-BV421, anti-Sca1-PE, and anti-lineage (Ter119, Gr1, Mac1, B220, Cd3e) antibody cocktail. Total RNA from FACS-sorted hematopoietic cells was isolated using TRIzol Reagent (Invitrogen) following the manufacturer's RNA isolation protocol. For preparation of NGS libraries polyadenylated mRNA from FASC-sorted c-Kit cells enriched from starting material of 100ng total RNA was processed utilizing NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: WT1
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: bone marrow
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: Wild-type 1
characteristics.characteristic: title