characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-01T00:15:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-01T00:15:25Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111468663
characteristics.characteristic: External Id
characteristics.text: Faculty of Biology, Medicine and Health, University of Manchester
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-01T00:15:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-01T00:15:25Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13562693
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12291:siADNP_rep1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: U2OS
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: U2OS cells were grown in DMEM medium containing 10% foetal bovine serum at 37°C incubator with 5% CO2. Cells were transfected using ADNP, TRIM28 or ZMYM2 siRNA or non-target control siRNA for 48 hours, followed by second siRNA transfection. Total RNA was extracted using an RNeasy plus kit (Qiagen, 74134) with DNase treatment according to the manufacturer’s protocol. RNA-seq libraries were constructed following the TruSeq Stranded mRNA sample preparation protocol (Illumina).
characteristics.characteristic: description
characteristics.text: juvenile stage
characteristics.characteristic: developmental stage
characteristics.text: osteosarcoma
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: tibia
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: siADNP_rep1
characteristics.characteristic: title