characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-14T00:17:33Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-14T00:17:33Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112162579
characteristics.characteristic: External Id
characteristics.text: Istituto di Ricerche Farmacologiche \"Mario Negri\" IRCCS
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-14T00:17:33Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-14T00:17:33Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14272376
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12394:Hs746T_xen_VEICOLO_3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Hs 746.T
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Xenograft protocol: Gastric cell lines are grown in the RPMI medium containing 10% fetal bovine serum. Sufficient number of the cells to be inoculated are harvested, intensively washed with PBS, and re- suspended in PBS. The number of the cells and the concentration are the followings: LMSU and NCI-N87 lines, 1x107 cells/0.2ml/flank; Hs746T line, 2.5x106 cells/0.2ml/flank. 2. Gastric cancer cells are subcutaneously inoculated on both flanks of 6-week old female nude mice (Nu/Nu) and let them grow until the size of the tumor reaches to 100-150 mm3 (about one week). 3. Tumor bearing mice are randomized according to the body weight and tumor size, and divided into two experimental groups (10-12 animals/ one group): treated with vehicle or with anti-tumor reagent (ATRA or AM580). 4. The animals are treated either with vehicle or anti-tumor reagents (15 mg/Kg of ATRA, or 0.3mg/Kg of AM580) for 5 days a week for 3 weeks. Body weight and the tumor size are monitored twice a week. 5. At the end of the treatment, animals are sacrificed, and tumor tissues are extracted and snap frozen. 6. RNA is extracted from the tumor tissues using miRNeasy Mini (Qiagen) kit and used for sample preparation for RNA sequencing analysis. Collection of 5-10 million cells in PDS, and resuspension of the pellet in a buffer. Protocol: miRNeasy Mini Kit TruSeq Stranded Total RNA Sample Preparation Guide
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: gastric carcinoma
characteristics.characteristic: disease
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: skeletal muscle
characteristics.characteristic: metastatic site
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: stomach
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Hs746T_xen_VEICOLO_3
characteristics.characteristic: title
characteristics.text: grown until neoplasm in mouse 2.5x106 cells/0.2ml/flank
characteristics.characteristic: xenograft