characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-16T00:26:35Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-16T00:26:35Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113482195
characteristics.characteristic: External Id
characteristics.text: Vinca Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-16T00:26:35Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-16T00:26:35Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15478289
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13009:Sample 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HEK293
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: After culture, cells were rinsed with PBS total RNA was isolated according to TRI Reagent protocol. Cell lines were maintained at 37˚C, 95% humidity and 5% CO2 in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Total RNA was isolated according to TRI Reagent protocol. The SMARTer smRNA-Seq Kit for Illumina is used to generate small RNA-seq libraries for sequencing on iSeq100 Illumina platform. A total of 500ng of total RNA was used as input for library construction. Illumina adapter and index sequences are incorporated in a ligation-free manner during library amplification, ensuring that diverse smRNA species are represented with minimal bias.
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: MIR185 knockout
characteristics.characteristic: genetic modification
characteristics.text: CRISPR-Cas9 induced MIR185 gene knockout
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: kidney
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 2
characteristics.characteristic: title