characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-19T20:23:50Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-19T20:23:50Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112824061
characteristics.characteristic: External Id
characteristics.text: Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-19T20:23:50Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-19T20:23:50Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14831597
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12796:d14D1
characteristics.characteristic: Submitter Id
characteristics.text: 25
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Sebastian
characteristics.characteristic: cultivar
characteristics.text: Protocols: For RNA seq analyses, plant tissue (2 cm long fragments of the second leaf located 3 cm below leaf tip) was collected in four biological replicates, each containing fragments from 4 seedlings of control and drought-stressed plants of both genotypes [here only drought-treated samples are deposited]. Tissue was immediately frozen in liquid nitrogen and stored in -80oC degrees Drought stress was applied according to the previously described protocol (Daszkowska-Golec et al., 2019). Briefly, plants were grown in a greenhouse for 10 days after sowing (DAS) under optimal water conditions (14% vwc), 20/18°C day/night, 16/8 photoperiod and 420 μE m−2 s−1 light intensity, in boxes (400 × 140 × 175 mm) filled with soil containing a mixture of sandy loam and sand (7:2) supplied with a nutrient medium. Next, the soil moisture was decreased by withholding the irrigation. On 15 DAS, the soil moisture decreased to 3%, and plants were moved into a growth chamber, where the temperature regime was set to 25°C/20°C day/night, with a 16/8 hr photoperiod and 420 μEm−2 s−1 light intensity (Figure 1A). The severe drought stress (3–1.5% vwc) was applied for the next 10 days (16–25 DAS). The control plants were grown under the same conditions with optimal water supply (14% vwc) parallel to the drought-treated plants. The soil moisture was measured daily using a Time-domain reflectometer EasyTest (Institute of Agrophysics, Polish Academy of Sciences, Poland). Drought stress was applied according to the previously described protocol (Daszkowska-Golec et al., 2019). Briefly, plants were grown in a greenhouse for 10 days after sowing (DAS) under optimal water conditions (14% vwc), 20/18°C day/night, 16/8 photoperiod and 420 μE m−2 s−1 light intensity, in boxes (400 × 140 × 175 mm) filled with soil containing a mixture of sandy loam and sand (7:2) supplied with a nutrient medium. Next, the soil moisture was decreased by withholding the irrigation. On 15 DAS, the soil moisture decreased to 3%, and plants were moved into a growth chamber, where the temperature regime was set to 25°C/20°C day/night, with a 16/8 hr photoperiod and 420 μEm−2 s−1 light intensity (Figure 1A). The severe drought stress (3–1.5% vwc) was applied for the next 10 days (16–25 DAS). The control plants were grown under the same conditions with optimal water supply (14% vwc) parallel to the drought-treated plants. The soil moisture was measured daily using a Time-domain reflectometer EasyTest (Institute of Agrophysics, Polish Academy of Sciences, Poland). RNA was extracted with the use of miRvana kit with phenol according to manufacturer’s protocol cDNA libraries were prepared following Illumina TruSeq standard procedures and eventually
characteristics.characteristic: description
characteristics.text: seedling development stage
characteristics.characteristic: developmental stage
characteristics.text: drought environment
characteristics.characteristic: envrionmental stress
characteristics.text: hvd14
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Hordeum vulgare
characteristics.characteristic: organism
characteristics.text: Hordeum vulgare
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.characteristic: organism part
characteristics.text: Hordeum vulgare
characteristics.characteristic: scientific_name
characteristics.text: d14D1
characteristics.characteristic: title