characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-27T04:00:06Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-27T04:00:06Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113476855
characteristics.characteristic: External Id
characteristics.text: Peter Doherty Institute for Infection & Immunity, University of Melbourne
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-27T04:00:06Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-27T04:00:06Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15472949
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12954:Naive (Supp Figure 5)
characteristics.characteristic: Submitter Id
characteristics.text: 6 to 12
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: erythrocyte
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Peripheral blood (5-6 drops) was collected into 1ml cold RPMI medium containing 5 U/mL heparin by tail vein bleed. Diluted blood (50 μL) was further diluted in 2 mL cold 1% BSA/PBS and RBCs isolated away from leukocytes by cell sorting. RBCs cells were then sorted as single-cells using the Chromium 10X Genomics platform. Approximately 5,000 RBCs (red blood cells), some of which were parasitized, were loaded per channel onto a Chromium controller (10x Genomics) for generation of gel bead-in-emulsions Sequencing libraries were prepared using Single Cell 3’ Reagent Kits v3 or where specified v2 (10x Genomics).
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Plasmodium berghei ANKA
characteristics.characteristic: infect
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: Naive (Supp Figure 5)
characteristics.characteristic: title