characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-16T00:26:33Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-16T00:26:33Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113426899
characteristics.characteristic: External Id
characteristics.text: Institut de Biologie Valrose, iBV UMR CNRS 7277/Inserm 1091/Universite Cote d’Azur
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-16T00:26:33Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-16T00:26:33Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15422025
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12938:NG-6612_R2_NI_lib21097_1481_2
characteristics.characteristic: Submitter Id
characteristics.text: 6.5
characteristics.unit: hour
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: common urchin
characteristics.characteristic: common name
characteristics.text: Protocols: Two independent batches of embryos were used for each condition. Embryos from control cultures (uninjected) mRNA were collected at the early blastula stage (6h30 after fertilization at 18°C). RNA was purified using Trizol. Libraries were made by the GATC company. Briefly, polyadenylated transcripts were purified by two rounds of oligo-dT chromatography and cDNA libraries were constructed using a Superscript double stranded cDNA synthesis kit (Invitrogen), fragmented by sonication, and cDNA fragments of 200-500 bp were gel purified. Illumina adaptaters were ligated to these fragments.
characteristics.characteristic: description
characteristics.text: blastula
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Paracentrotus lividus
characteristics.characteristic: organism
characteristics.text: Paracentrotus lividus
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: wild type
characteristics.characteristic: phenotype
characteristics.text: B
characteristics.characteristic: replicate
characteristics.text: Paracentrotus lividus
characteristics.characteristic: scientific_name
characteristics.text: non injected
characteristics.characteristic: stimulus
characteristics.text: NG-6612_R2_NI_lib21097_1481_2
characteristics.characteristic: title