characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-27T00:17:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-27T00:17:08Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113577834
characteristics.characteristic: External Id
characteristics.text: Shandong Provincial Key Laboratory of Synthetic Biology, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-27T00:17:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-27T00:17:08Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15572748
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13020:A2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Protocols: cell pellets from 50 ml culture were collected by centrifugation at –4 °C and 9,000 rpm (Hitachi CF16RXII, Qingdao, China) for 5 min. Afterwards, pellets were frozen in liquid nitrogen and stored at–80°C. Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer’s protocol. RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The samples with RNA Integrity Number (RIN) ≥ 7 were subjected to the subsequent analysis. Double stranded cDNA was generated using the SuperScript Double-Stranded cDNA synthesis kit (Invitrogen) according to the manufacturer’s instructions.It was performed by Allwegenetech Corp. (Beijing, China) The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions. This part of experiment is carried out at Allwegenetech Corp. (Beijing, China).
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Clostridium ljungdahlii
characteristics.characteristic: organism
characteristics.text: Clostridium ljungdahlii
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Clostridium ljungdahlii
characteristics.characteristic: scientific_name
characteristics.text: A2
characteristics.characteristic: title