characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-30T00:24:16Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-30T00:24:16Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112484108
characteristics.characteristic: External Id
characteristics.text: Department of Biomedical Sciences, University of Copenhagen, Denmark
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-30T00:24:16Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-30T00:24:16Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14601458
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12573:2D control replicate 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: H9
characteristics.characteristic: cell_line
characteristics.text: cardiocyte
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells from 2D culture were scraped in RLT buffer (QIAGEN RNeasy kit). Organoids were dissolved in RLT buffer and passed 10-times through a 21G needle. Note: the \"replicate 1\" of 2D control and PD have 3 data files, corresponding to 3 differentiation runs carried out at the same time, e.g. derived from the same cell populations. QIAGEN RNeasy kits (Mini for 2D culture and Micro for organoids) according to manufacturer's handbook. Library was prepared with NEBNext Ultra II RNA Library Prep Kit (New England BioLabs). For the 2D cultures, 500 ng of RNA were used and 5 cycles of PCR amplification were performed, whereas for the organoids, 120 ng RNA were used and 9 cycles of PCR amplification were performed. AMPure XP (Beckman Coulter) was used for purification. Fragment Analyzer (Agilent) was used for quality assessment.
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: 2D adherent cell culture
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: inner cell mass
characteristics.characteristic: organism part
characteristics.text: embryonic stem cell
characteristics.characteristic: progenitor cell type
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 2D control replicate 1
characteristics.characteristic: title