characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-16T07:00:06Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-16T07:00:06Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112156252
characteristics.characteristic: External Id
characteristics.text: Department of Microbiology and Molecular GeneticsIMRIC, Faculty of Medicine, The Hebrew University of Jerusalem
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-16T07:00:06Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-16T07:00:06Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14266149
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12429:Sample 6
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Cultures ofE. coliMG1655 were grown at 37°C in either rich LB medium or minimal E medium pH 7.019supplied with 0.4% glucose (EG medium). At OD600of ~0.4the cells were collected. Total RNA was extracted, using TRI-reagent Term-seq libraries were constructed according to Dar et al., 2016, with slight changes. 1 μg of DNase treated total RNA was ligated to a 3' barcoded adapter (150 pmole) using T4 RNA ligase 1, in a 20 μl reaction at 22°C. Ligation reaction was arrested after two hours11by addition of 60 μl RLT buffer (Qiagen). The adapter ligated RNA samples were pooled together and cleaned-up using the Zymo Clean and ConcentratorTM-5 kit. The RNA was then fragmented using Ambion Fragmentation Reagent (Ambion AM8740),and cleaned-up using 2.5 volumes of RNAClean XP beads (Beckman-Coulter) and 1.5 volumes of isopropanol, according to manufacturer instructions. The RNA was depleted of ribosomal RNA using Ribo-Zero, cleaned-up using 2.5 volumes of RNAClean XP beads (Beckman-Coulter) and 1.5 volumes of isopropanol and then used for cDNA synthesis using Invitrogen SuperScript™ III First-Strand Synthesis System.The cDNA was cleaned-uptwice with 1.5 volumes of Ampure XP (Beckman-Coulter) and amplified by 9 cycles of PCR.
characteristics.characteristic: description
characteristics.text: exponential phase
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: rich LB medium
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Escherichia coli str. K-12 substr. MG1655
characteristics.characteristic: organism
characteristics.text: Escherichia coli str. K-12 substr. MG1655
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Escherichia coli str. K-12 substr. MG1655
characteristics.characteristic: scientific_name
characteristics.text: Sample 6
characteristics.characteristic: title