characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-20T00:19:23Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-20T00:19:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112721162
characteristics.characteristic: External Id
characteristics.text: VANDERBILT UNIVERSITY
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-20T00:19:23Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-20T00:19:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14718728
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12719:3575-LT-1
characteristics.characteristic: Submitter Id
characteristics.text: embryonic day 9.0 to 9.5
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Embryos were dissected in ice-cold PBS and staged by Theiler’s criteria. Embryos of the same genotype within E9.0 to E9.5 range were pooled, incubated for 10 minutes at 37oC with Accumax (Millipore Sigma, #A7089) supplemented with DNase (Life technologies, #AM2222; 1:1,000 dilution ratio), and further disaggregated by vigorous pipetting during incubation. Passing the cell suspension through 35 µm strainer caps (Corning, #352235) led to single cells, which were washed with FACS buffer (R&D systems, #FC001) and pelleted at 4oC, 1000 rpm for 3 mins. Cells were resuspended in FACS buffer containing DNaseI (1:1,000 dilution) and stained with 7AAD (15 mins, room temperature - RT). Live cells (7AAD-negative) expressing either GFP or TdTomato fluorescence were then sorted into DMEM with 10% FBS and further centrifuged and resuspended at 600 cells/μl concentration required for sequencing step. RNA was extracted according to the manufacturer’s instructions for 10x Genomics Single Cell 3′ Library kit v3 Libraries were constructed according to the manufacturer’s instructions for 10x Genomics Single Cell 3′ Library kit v3
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: Sox17.GFPCre heterozygous; R26.WT homozygous
characteristics.characteristic: genotype
characteristics.text: 1
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: embryo
characteristics.characteristic: organism part
characteristics.text: GFP-positive
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: mixed
characteristics.characteristic: sex
characteristics.text: CD1
characteristics.characteristic: strain
characteristics.text: 3575-LT-1
characteristics.characteristic: title