characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-01T00:20:48Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-01T00:20:48Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113611642
characteristics.characteristic: External Id
characteristics.text: University of Glasgow, School of Cancer Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-01T00:20:48Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-01T00:20:48Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15605821
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13077:Sample 33
characteristics.characteristic: Submitter Id
characteristics.text: 78
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Whole sections (~100 mm2 x 0.5 μm) of formalin-fixed paraffin-embedded colon cancer tissue was scrapped into wells of a PCR plate. TempO-Seq Lysis Buffer was added to tissues in PCR plate, and the sample was overlaid with mineral oil. After deparaffinization by heating, the tissue was lysed using TempO-Seq Protease mix. The lysate was then combined with a mixture of detector oligonucleotides (DOs), designed as pairs that anneal adjacent to one another on the target RNAs. After a hybridization step, unbound DOs were degraded in an enzymatic step, and the bound DOs were ligated into a complete probe sequence. The ligated probes were amplified in a PCR step, purified, and combined in an indexed multiplex library.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: colon cancer
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: colon
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: Sample 33
characteristics.characteristic: title