characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-31T00:17:52Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-31T00:17:52Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110422725
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF MANCHESTER
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-31T00:17:52Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-31T00:17:52Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12521095
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12024:RR50
characteristics.characteristic: Submitter Id
characteristics.text: 13
characteristics.unit: month
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: four-striped grass mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Animals were sedated with isoflurane and immediately culled by cervical dislocation at ZT2 or ZT14. Retina, lung and liver tissues was dissected and snap frozen immediately. For SCN samples, punches were collected from 1 mm thick brain slices containing a sample corer. All samples were stored at -80°C until RNA extraction. For retina, lung and liver samples total RNA was extracted from < 5 mg frozen tissue using the Qiagen RNeasy Mini kit (Qiagen 74104). For SCN, total RNA was extracted from snap-frozen brain punches using the Qiagen RNeasy Micro kit (Qiagen 74004). All samples were quantified using a 4200 TapeStation (Agilent Technologies). For retina, lung and liver samples, cDNA libraries were generated using the Illumina Stranded mRNA Prep Ligation kit (Illumina, Inc. 20040532). For SCN samples, cDNA libraries were prepared using the SMARTer Stranded Total RNA-Seq Kit V3 - Pico Input Mammalian (Takara Bio 634485).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: 12 hour light-dark cycle
characteristics.characteristic: growth condition
characteristics.text: M10
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Rhabdomys pumilio
characteristics.characteristic: organism
characteristics.text: Rhabdomys pumilio
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Rhabdomys pumilio
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: RR50
characteristics.characteristic: title