characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-10-12T09:00:10Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-01T00:20:14Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110473249
characteristics.characteristic: External Id
characteristics.text: Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen
characteristics.characteristic: INSDC center name
characteristics.text: 2022-10-12T09:00:10Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-01T00:20:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12570941
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12048:Sample 50
characteristics.characteristic: Submitter Id
characteristics.text: 15
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: The mice were fasted overnight (11 hours) prior to liver isolation, and livers were kept at -80°C until analysis. For eight weeks, female C57BL/6JRj mice (seven weeks old at the start of treatment) received, by subcutaneous injections, either a glucagon receptor antibody (GCGR Ab, REGN1193, 10 mg/kg body weight; Regeneron; IC50: 3.5 pM (Okamoto et al., 2015)) or a control antibody (Ctl Ab, REGN1945, 10 mg/kg body weight; Regeneron). The antibodies were administered once weekly (Wednesdays at 8 AM). The last doses of GCGR Ab and Ctl Ab were given one week prior to termination. Raw sequencing data were processed using bcl2fastq software version 2.20.0 (Illumina). Total DNA/RNA was purified by an AllPrep DNA/RNA Minikit (80204, QIAgen) according to manufacturer’s instructions and quality tested using a 2100 Bioanalyzer instrument (Agilent Genomics). The extract was DNase treated with RNeasy Minikit (74106, QIAgen) and RNase-Free DNase set (79254, QIAgen) according to manufacturer’s protocol. All the above-mentioned processes were performed on a QIACube machine (QIAgen). Library preparation was done using TruSeq Stranded Total RNA Library Prep Gold (20020599, Illumina) and IDT for Illumina-TruSeq RNA UD Indexes (20022371, Illumina) according to the manufacturer’s instructions. Some samples (input <100 ng) were prepared with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB-E7760S, New England Biolabs), NEBNext rRNA Depletion Kit 24 with beads (NEB-E7765, New England Biolabs), and NEBNext Multiplex Oligos for Illumina (E7600S, New England Biolabs).
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: C57BL/6JRj
characteristics.characteristic: strain
characteristics.text: Sample 50
characteristics.characteristic: title