characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-01T00:20:33Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-01T00:20:33Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112655566
characteristics.characteristic: External Id
characteristics.text: Department of Medicine 1, Friedrich Alexander University, Erlangen-Nuremberg, Germany
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-01T00:20:33Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-01T00:20:33Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14653121
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12662:No_163
characteristics.characteristic: Submitter Id
characteristics.text: 8
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: After removing the medium, organoids were washed 3 x with 1ml PBS w/o Ca++/Mg++ and then collected for RNA extraction. Total RNA was isolated using the PeqGold microspin total RNA Kit following manufacturers' instructions (Peqlab/VWR). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Stat1 KO Stat2 KO
characteristics.characteristic: genotype
characteristics.text: intestinal organoid
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: small intestine
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: No_163
characteristics.characteristic: title