characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-30T00:24:23Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-30T00:24:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112864438
characteristics.characteristic: External Id
characteristics.text: Institute of Chemical Biology, National Hellenic Research Foundation
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-30T00:24:23Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-30T00:24:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14871089
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12816:2A
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: RAW 264.7
characteristics.characteristic: cell_line
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: RAW264.7 cells were cultured for 48 h in 6-well plates (Greiner) in phenol red-free alpha MEM supplemented with 10% dextran-coated charcoal-stripped FBS with ultra-low endotoxin levels (Biosera). Cells were treated with vehicle (0.1% DMSO), dexamethasone (1 μM) or AZD9567 (1 μΜ) in the presence or absence of lipopolysaccharide (LPS, 10 ng/ml) for 1 h. At the end of the treatment cells were collected in TRIzol Reagent and used for RNA extraction. RNA was extracted from cells using the TRIzol Reagent (Invitrogen) followed by a clean-up step combined with on-column DNase digestion using the RNeasy kit (Qiagen) according to manufacturers&#39; instructions. After the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) are used to isolate mRNA. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. The suitable fragments are selected for the PCR amplification as templates.
characteristics.characteristic: description
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: ascitic fluid
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: BALB/c
characteristics.characteristic: strain
characteristics.text: 2A
characteristics.characteristic: title