characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-27T15:00:48Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-27T15:00:48Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112868273
characteristics.characteristic: External Id
characteristics.text: The University of Tokyo, Institute for Quantitative Biosciences
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-27T15:00:48Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-27T15:00:48Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14874928
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12819:Sample 2_GEX
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: mixed K562 and THP-1
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: K562 cells and THP-1 cells were purchased from JCRB CellBank (JCRB0019 lot: 06212017, JCRB0112.1 lot:11092017). Cells were grown in RPMI1640 medium (Sigma-Aldrich) supplemented with 10% FBS (Sigma-Aldrich) and 1x Antibiotic Antimycotic solution (Thermo Fisher Scientific). Cells were encapsulated in microdroplets with DNA-barcoded beads, where DNA barcodes were released from beads and absorbed by cells. cDNA and DNA barcodes were read in separate sequencing libraries. Droplet-based (Chromium NextGEM SIngle Cell 3' Reagent Kits, 10X) Droplet-based (Chromium NextGEM single Cell 3' Reagent Kits, 10X) used for the GEX libraries. For DNA barcode samples, libraries were also prepared in parallel to cDNA samples using the same 10X kit but generated based on a custom cell-hashing method. This cell-hashing method does not tag samples (one barcode)-by-(one sample) basis. Instead, it tags samples with unique ID generated by combinations of barcodes . See Additional File E-MTAB-12819.vsc for a list of each barcode consisting of the combinatorial IDs.
characteristics.characteristic: description
characteristics.text: mixed chronic myelogenous leukemia and acute monocytic leukemia
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: mixed bone marrow and blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 2_GEX
characteristics.characteristic: title