characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-15T00:19:51Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-15T00:19:51Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110471358
characteristics.characteristic: External Id
characteristics.text: Insitute of Oncology Research (IOR)
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-15T00:19:51Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-15T00:19:51Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12569381
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12031:VCaP-Sh1-MAT2A_3.fastq.gz
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: VCaP
characteristics.characteristic: cell_line
characteristics.text: prostate gland cancer cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: VCaP cells were obtained from American Type Culture Collection and maintained in their media. DMEM [ATCC, cat #30-2002] supplemented with 10% fetal bovine serum (FBS, Biowest, cat #S181B) and 1% Pen/Strep (Thermo Fisher, cat #15070063) was used for VCaP. Cells were regularly checked for mycoplasm contamination using MycoAlert Mycoplasma detection kit. VCaP with stable knockdown of MAT2A were generated by viral infection of control (Ctrl) and MAT2A shRNAs (Sh1 and Sh2) [Sigma-Aldrich], followed by subsequent selection with Puromycin [Sigma-Aldrich]. Collection of 5 million cells in PBS RNA was isolated from RWPE cells in three independent replicates, marked with Illumina total prep 96 RNA amplification kit (Ambion). RNA sequencing was performed using Next Ultra II Directional RNA Library Prep Kit for Illumina starting from 800ng of total RNA each sample and sequenced on the Illumina NextSeq500 with single-end, 75 base pair long reads
characteristics.characteristic: description
characteristics.text: prostate cancer
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: prostate gland
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: VCaP-Sh1-MAT2A_3.fastq.gz
characteristics.characteristic: title