characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-30T00:24:16Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-30T00:24:16Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112483440
characteristics.characteristic: External Id
characteristics.text: Wageningen University and Research
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-30T00:24:16Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-30T00:24:16Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14600791
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12575:Sample 24
characteristics.characteristic: Submitter Id
characteristics.text: 72
characteristics.unit: hour
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: The Caenorhabditis elegans nematodes were grown on petri-dishes with nematode growth medium, feeding off Escherichia coli OP50. A new population was started by transferring clean eggs to a fresh dish. After 72 hours at 20°C, nematodes were collected by washing off with M9 buffer. Pellets with nematodes were allowed to settle by gravity and washed three times to remove external bacteria. Pellets were flash-frozen in liquid nitrogen and stored at -80°C until further processing. DNA was extracted using the DNeasy® 96 Blood & Tissue Kit (QIAGEN, Germany) according to the manufacturers specifications. For each sample 0.75 ng of DNA was combined with 2.5 µl 1/35th diluted transposome (Illumina; # FC-121-1011), and 1X Tris Buffer in a 10 µl final volume on ice. This reaction was incubated at 55°C for 10 minutes. Subsequently the DNA was amplified. This reaction contained for each sample (final concentrations): 1X ExTaq Buffer, 0.2 mM dNTPs, 1 Unit ExTaq (Takara, catalog #RR001A), 0.2 µM primer 1, 0.2 µM primer 2, and 5 µl of tagmentation material from the previous step in a 25 µl total volume. Each sample had a unique pair of indexed primers. Amplification was carried out in a thermocycler with the following conditions: 72°C for 3 minutes (1X); 95°C for 30 seconds (1X); 95°C 10 seconds, 62°C 30 seconds, 72°C 3 minutes (20X); 10°C hold. Then, 16 µl from each amplification reaction was used to generate a pool of 48 libraries. Amplicons were gel extracted in the size range of 500-700 bp, and gel purified the sample with Qiagen's Gel Purification Kit (catalog #28706).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: WN437
characteristics.characteristic: genotype
characteristics.text: WN437
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: organism
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: scientific_name
characteristics.text: hermaphrodite
characteristics.characteristic: sex
characteristics.text: WN437
characteristics.characteristic: strain
characteristics.text: Sample 24
characteristics.characteristic: title