characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-08-30T06:23:33Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-08-30T06:23:33Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112966557
characteristics.characteristic: External Id
characteristics.text: NOFIMA
characteristics.characteristic: INSDC center name
characteristics.text: 2023-08-30T06:23:33Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-08-30T06:23:33Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14961584
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12870:Ne68
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Atlantic cod
characteristics.characteristic: common name
characteristics.text: Protocols: Samples were collected 1) the day before the temperature was increased, 2) when the water temperature reached 17oC and 3) 5 weeks post infection. Fish (n=6, length = 23±2cm; weight =114±33g) were dip netted from the tank and were euthanised with an overdose of Benzoak Vet. The olfactory organ was exposed by opening the olfactory cavity via the nostril. The right olfactory organ was immediately placed in BiopSafe containing 10% formalin (Mermaid Medical, Vedbæk, Denmark) for histological processing. On the other hand, the left olfactory organ was placed in RNAlater® (Ambion, Inc., Connecticut, USA), allowed tissue penetration at 4oC overnight and stored at -40oC prior to RNA isolation. Total RNA was isolated from the olfactory organ using the Agencourt RNAdvance™ Tissue Total RNA Purification Kit (Beckman Coulter Inc., CA, USA). The purified RNA was quantified using NanoDrop 8000 Spectrophotometer (ThermoFisher Scientific, United States) and integrity was further assessed using the Agilent® 2100 Bioanalyzer™ RNA 6000 Nano Kit (Agilent Technology Inc., Santa Clara, CA, USA). All samples had an RNA Integrity Number (RIN) above 7.0. Total RNA samples were sent to the Norwegian Sequecing Center (OUS, Norway). The strand-specific TruSeq RNA Library Prep Kit (Illumina, CA, USA) was used for preparation of RNA-seq libraries, using the manufacturer’s protocol.
characteristics.characteristic: description
characteristics.text: Francisella noatunensis
characteristics.characteristic: infect
characteristics.text: Gadus morhua
characteristics.characteristic: organism
characteristics.text: Gadus morhua
characteristics.characteristic: organism
characteristics.text: olfactory bulb
characteristics.characteristic: organism part
characteristics.text: Gadus morhua
characteristics.characteristic: scientific_name
characteristics.text: water temperature 12 oC
characteristics.characteristic: stimulus
characteristics.text: Ne68
characteristics.characteristic: title