characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-01T00:20:10Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-01T00:20:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110278092
characteristics.characteristic: External Id
characteristics.text: Quadram Institute Bioscience
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-01T00:20:10Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-01T00:20:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12375706
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11917:ha66
characteristics.characteristic: Submitter Id
characteristics.text: 66
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Hepatic artery samples were collected from patients at the time of liver transplantation at Addenbrookes NHS Trust, Cambridge University Hospitals, Cambridge, UK. Hepatic arterial samples were collected from patients with confirmed cirrhosis and clinically significant portal hypertension with a diagnosis of primary sclerosing cholangitis involving both intra- and extra-hepatic bile ducts, or alcohol-related liver disease or non-alcohol related fatty liver disease. The hepatic artery samples were identified and resected during surgery by the attending transplant surgeon. The samples were approximately 1 cm in length and were taken from the main artery at the hilum of the liver, just proximal to the bifurcation into the left and right hepatic artery. The proximity to the bifurcation was chosen for consistency and to ensure the same area was sampled across all specimens. Once collected, samples were frozen in liquid nitrogen and stored at -80°C prior to further analysis to allow for batched processing. RNA was extracted with the Qiagen RNeasy Mini Kit following the manufacturer’s protocol. Nucleic acid library construction was performed by the Sanger Institute
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: ha66
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: hepatic artery
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: ha66
characteristics.characteristic: title