characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-31T00:17:47Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-31T00:17:47Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110358626
characteristics.characteristic: External Id
characteristics.text: BAH-GU
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-31T00:17:47Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-31T00:17:47Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12456334
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11955:S_260
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: ER- BC patients with ductal carcinoma who underwent curative tumour resection surgery in Glasgow Royal Infirmary, Western Infirmary, or Stobhill Hospitals (Glasgow, UK) between 1995 and 1998 were included in the study. Resected tumour tissue was formalin-fixed and paraffin-embedded. FFPE tissue was deparaffinised prior to tissue digestion. The tissue lysate was combined with detector oligos which were annealed in immediate juxtaposition to each other on the targeted RNA template and ligated. Amplification of ligated oligos was performed using a unique primer set for each sample, introducing a sample-specific barcode and Illumina adaptors.
characteristics.characteristic: description
characteristics.text: estrogen-receptor negative breast cancer
characteristics.characteristic: disease
characteristics.text: cancer
characteristics.characteristic: disease
characteristics.text: lymph node status negative
characteristics.characteristic: disease staging
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: breast
characteristics.characteristic: organism part
characteristics.text: CAIX expression low
characteristics.characteristic: phenotype
characteristics.text: neoplasm
characteristics.characteristic: sampling site
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: S_260
characteristics.characteristic: title