characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-30T00:24:23Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-30T00:24:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112868472
characteristics.characteristic: External Id
characteristics.text: Genomix4Life SRL
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-30T00:24:23Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-30T00:24:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14875127
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12828:BC4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: mononuclear cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: PBMCs separation was performed using Ficoll-Paque (Ficoll Plaque PLUS – GE Healthcare Life Sciences, Piscataway, USA) RNA was extracted using TRIzol reagent (TRIzol Reagent, Invitrogen Life Technologies, Carlsbad, CA, USA), according to the manufacturer’s instructions Indexed libraries were prepared from 500ng/ea purified RNA with Illumina Stranded mRNA (Illumina) Library Prep Kit according to the manufacturer’s instructions. For library quantifications, TapeStation 4200 (Agilent Technologies) and Qubit (Thermo Fischer) was used.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: urinary bladder cancer
characteristics.characteristic: disease
characteristics.text: 4
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: BC4
characteristics.characteristic: title