characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-30T00:24:27Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-30T00:24:27Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113425367
characteristics.characteristic: External Id
characteristics.text: Biosante UMR 1292 Health Department (DS) Interdisciplinary Research Institute of Grenoble (IRIG)
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-30T00:24:27Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-30T00:24:27Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15420493
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12928:A1T1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: ACHN
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Renal orthotopic implantation was carried out by injection of 1.106 786-O luc or ACHN luc cells into the left kidney of athymic nude mice. Tumor growth was followed by intraperitoneal injection of 150 mg/kg D-luciferin Potassium salt using IVIS Imager (Perkin Elmer). For tumoroid generation, cell dissociation was performed thanks to the gentleMACS Dissociator and the Tumor dissociation kit (Miltenyl Biotec). Dissociated cells were counted and coated with magnetic NanoShuttle (Greiner) (1 µL per 20 000 cells). Tumoroids were cultured for 14 days in DMEM media supplemented with 10 % FBS (GIBCO), 100 U/mL penicillin + streptomycin (Invitrogen). Total RNA was extracted from treated cells using the MirVana PARIS kit (Thermofisher). The 3′ Bulk RNA Barcoding and sequencing (BRB-seq) experiments were performed at the Research Institute for Environmental and Occupational Health (Irset, Rennes, France) according to the published protocol (Alpern et al., 2019).The sequencing libraries were built by tagmentation using 50 ng of ds cDNA with Illumina Nextera XT Kit (Illumina, #FC-131-1024)
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: papillary renal cell carcinoma
characteristics.characteristic: disease
characteristics.text: tumoroid culture
characteristics.characteristic: growth condition
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: kidney
characteristics.characteristic: organism part
characteristics.text: tumoroid
characteristics.characteristic: sampling site
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: not available
characteristics.characteristic: sex
characteristics.text: A1T1
characteristics.characteristic: title
characteristics.text: ACHN cell implanted in kidney of athymic nude mice
characteristics.characteristic: xenograft