characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-30T00:24:21Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-30T00:24:21Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112797763
characteristics.characteristic: External Id
characteristics.text: Massachusetts General Hospital Harvard Medical School Broad Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-30T00:24:21Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-30T00:24:21Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14805309
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12778:Donor C CAR
characteristics.characteristic: Submitter Id
characteristics.text: 30
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: T cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: CAR T cells were generated from PBMCs of healthy donors under IL7&IL15 supplementation. Retroviral transduction with CAR and CAR.CCR7 was performed at day 3&4 respectively after 48 hrs of plate-bound (CD3/CD28) activation. Cells were cultured until day 10 and either cultured over night with or without Jeko1 co-culture. Cells were enriched at an AriaFusion cell sorter by using the fluorescent reporter mTurquoise that is incorporated in the vector backbone. Cells were sorted into a 15mL conical tube with 5mL fully supplemented medium, spun down at 400xg for 5 min. and directly processed for mRNA Seq RNA was harvested using the Uneasy Micro Kit from Qiagen strictly following the manufacturers instructions. All steps were performed on ice and on an RNAse free bench Illumina TruSeq stranded RNA was used to generate libraries. All libraries were generated with 1µg total input RNA per sample
characteristics.characteristic: description
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: monoculture
characteristics.characteristic: growth condition
characteristics.text: C
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: T cell
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: Donor C CAR
characteristics.characteristic: title