characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-08-25T00:41:19Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-08-25T00:41:19Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112702283
characteristics.characteristic: External Id
characteristics.text: UCL Great Ormond Street Institute of Child Health
characteristics.characteristic: INSDC center name
characteristics.text: 2023-08-25T00:41:19Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-08-25T00:41:19Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14699849
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12703:KO-AAVNDP1
characteristics.characteristic: Submitter Id
characteristics.text: 2
characteristics.unit: month
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mice were sacrificed by cervical dislocation. The inner ear was immediately isolated from surrounding tissue and the cochlea was separated from the vestibular region, the stapedial artery removed and the cochlea snap frozen Total RNA was extracted using a modification of a published protocol (PMID 25818515) (TRI Reagent® 93289-25ML Sigma-Aldrich, DirectZol kit). RNA was eluted in 40-50 μl of nuclease-free water and analysed using the by NanoDropTM 2000 (Thermo Scientific) and Agilent Bioanalyzer platforms. LIbraries were constructed for Illumina, strand-specific RNA-seq with PolyA selection according to standard protocols by Azenta Lifesciences
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Norrie disease
characteristics.characteristic: disease
characteristics.text: Ndp-KO
characteristics.characteristic: genotype
characteristics.text: GT17_M3
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: cochlea
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL
characteristics.characteristic: strain
characteristics.text: KO-AAVNDP1
characteristics.characteristic: title
characteristics.text: AAV NDP gene therapy
characteristics.characteristic: treatment