characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-08-22T20:40:49Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-08-22T20:40:49Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111379099
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF MANCHESTER
characteristics.characteristic: INSDC center name
characteristics.text: 2023-08-22T20:40:49Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-08-22T20:40:49Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13476084
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12196:Sample 10
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: A375
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Growth conditions - This submission studied the effect of IFNγ stimulation on 6 cancer cell lines. A375 (https://www.cellosaurus.org/CVCL_0132) and 501-mel (https://www.cellosaurus.org/CVCL_4633) were human melanoma cell lines. B16-F10 (https://www.cellosaurus.org/CVCL_0159), 5555 (provided by Santiago Zelenay, The University of Manchester) and YUMM2.1 (https://www.cellosaurus.org/CVCL_JK30) mouse melanoma cell lines. MC38 (https://www.cellosaurus.org/CVCL_B288) mouse colon cancer cell line. Human cell lines were maintained in DMEM (high glucose) medium. Mouse cell lines were maintained in RPMI-1640 (Sigma-Aldrich) medium. All growth media were supplemented with 10% fetal bovine serum (FBS; Life Technologies), 1% penicillin-streptomycin (P/S; Sigma-Aldrich). All cells were maintained under standard conditions at 37°C in a 5% CO2 humidified incubator and passaged before reaching confluency. Cell cultures were routinely tested for mycoplasma contamination by PCR. Cells were passaged with or without IFNγ for at least 2 weeks, samples are then harvested for RNA-seq. Human cells (A375) were treated with 20 IU/ml IFNγ, mouse cells with 50 IU/ml IFNγ, replacing medium and IFNγ every 72 h with passaging of cells as necessary for 2 weeks. Recombinant human IFNγ (PHC4031) and recombinant mouse IFNγ (PMC4031) were purchased from Gibco. Growth conditions - This submission studied the effect of IFNγ stimulation on 6 cancer cell lines. A375 (https://www.cellosaurus.org/CVCL_0132) and 501-mel (https://www.cellosaurus.org/CVCL_4633) were human melanoma cell lines. B16-F10 (https://www.cellosaurus.org/CVCL_0159), 5555 (provided by Santiago Zelenay, The University of Manchester) and YUMM2.1 (https://www.cellosaurus.org/CVCL_JK30) mouse melanoma cell lines. MC38 (https://www.cellosaurus.org/CVCL_B288) mouse colon cancer cell line. Human cell lines were maintained in DMEM (high glucose) medium. Mouse cell lines were maintained in RPMI-1640 (Sigma-Aldrich) medium. All growth media were supplemented with 10% fetal bovine serum (FBS; Life Technologies), 1% penicillin-streptomycin (P/S; Sigma-Aldrich). All cells were maintained under standard conditions at 37°C in a 5% CO2 humidified incubator and passaged before reaching confluency. Cell cultures were routinely tested for mycoplasma contamination by PCR. Cells were passaged with or without IFNγ for at least 2 weeks, samples are then harvested for RNA-seq. Human cells (A375) were treated with 20 IU/ml IFNγ, mouse cells with 50 IU/ml IFNγ, replacing medium and IFNγ every 72 h with passaging of cells as necessary for 2 weeks. Recombinant human IFNγ (PHC4031) and recombinant mouse IFNγ (PMC4031) were purchased from Gibco. Total RNA was isolated using the RNAeasy mini kit. RNA integrity was assessed on the Agilent 2200 TapeStation (Agilent Technologies). TruSeq Stranded mRNA assay (Illumina)
characteristics.characteristic: description
characteristics.text: melanoma
characteristics.characteristic: disease
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: skin
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 10
characteristics.characteristic: title