characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-08-23T00:22:48Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-08-23T00:22:48Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111329091
characteristics.characteristic: External Id
characteristics.text: Zhejiang chinese medical university
characteristics.characteristic: INSDC center name
characteristics.text: 2023-08-23T00:22:48Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-08-23T00:22:48Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13426656
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12143:YYTN-1
characteristics.characteristic: Submitter Id
characteristics.text: 8
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Norway rat
characteristics.characteristic: common name
characteristics.text: Protocols: Brain tissue samples were ground into cell powder with liquid nitrogen and transferred to Trizol lysis solution to allow complete lysis of tissue cells. The disrupted tissue samples were centrifuged for 5 minutes at 12000 g at 4°C. The supernatant was then added to 300 μL chloroform/isoamyl alcohol (24:1), sonicated to 1200 g for 8 minutes at 4°C, and centrifuged at 12000g for 8 minutes. After 2 hours, the supernatant was stored in the refrigerator at - 20°C in an isopropanol centrifuge tube containing 600 μL. The supernatant was discarded after centrifugation at 17500g at 4°C for 25 minutes. Finally, the pellet was washed with 0.9 ml of 75% ethanol, suspended upside down, and centrifuged at 17500g for 3 min at 4℃. For total RNA, the supernatant was discarded, dried for 3-5 minutes, and then washed with 30-200μL of RNase-free water. PolyA tail mRNAs were enriched with OligodT magnetic beads. An appropriate amount of interrupting reagent was added to the obtained mRNA, which was allowed to break at a high temperature. Single-stranded cDNA was synthesized using the interrupted mRNA as a template. A double-strand synthesis reaction system is then configured to synthesize double-stranded cDNA. Purification and recovery of cDNA were accomplished with kits, adding base \" A \" to the 3 'end of cDNA and ligate linkers, followed by fragment size selection and amplification by PCR.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: stroke
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Rattus norvegicus
characteristics.characteristic: organism
characteristics.text: Rattus norvegicus
characteristics.characteristic: organism
characteristics.text: brain
characteristics.characteristic: organism part
characteristics.text: Rattus norvegicus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: Sprague Dawley
characteristics.characteristic: strain
characteristics.text: YYTN-1
characteristics.characteristic: title