characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-01T00:20:11Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-01T00:20:11Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110369861
characteristics.characteristic: External Id
characteristics.text: CNRS, University Paris I Pantheon-Sorbonne & Gustave Roussy
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-01T00:20:11Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-01T00:20:11Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12468058
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11960:Lin_P95_7
characteristics.characteristic: Submitter Id
characteristics.text: 5
characteristics.unit: month
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Lin- lymphocyte
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Bone marrow megakaryocytes were sorted from recipient mice 8-12 weeks after tamoxifen feeding. Lin- cells were enriched by immunomagnetic negative selection (IMag; BD Biosciences) using Ter119, CD3, B220, Cd11b and Gr1 antibodies before being labeled with PE-conjugated anti-CD41 (clone MCReg30, BD) and APC-conjugated anti-CD42d (clone 1C2, Biolegend) antibodies. CD41+ CD42d+ cells were sorted using Influx 5 laser BD cell sorter in Trizol LS and stored at -80°C RNA isolation was performed using RNA isolation protocol with chloroform and GenElute LPA (Sigma) protocol cDNA Libraries were generated using the SMART-Seq® v4 Ultra® Low Input RNA and final libraries where generated with TruSeq Sample Preparation v.2 Guide (Illumina).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Srsf2-P95H
characteristics.characteristic: genotype
characteristics.text: lineage negative
characteristics.characteristic: immunophenotype
characteristics.text: P95_7
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: Lin_P95_7
characteristics.characteristic: title