characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-30T00:24:29Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-30T00:24:29Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113611606
characteristics.characteristic: External Id
characteristics.text: ICL-CFB
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-30T00:24:29Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-30T00:24:29Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15605785
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13078:EcN_Low_Rep1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Protocols: Bacteria were grown in Luria-Bertani broth at 37 degrees with shaking until early stationary phase. 4 ml of sample was mixed at a 1:2 ratio with RNAprotect from Quiagen and incubated at room temperature for 5 minutes. Samples were then centrifuged at 4 degrees for 10 minutes; the supernatant was discarded and the pellet stored at -80 degrees prior to RNA extraction. RNA extracted using the PureLink RNA Mini Kit from Invitrogen Library was constructed by the Core Unit Systems Medicine at the University of Würzburgas a contracted service.
characteristics.characteristic: description
characteristics.text: Pmcc-Low plasmid
characteristics.characteristic: genetic modification
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Escherichia coli
characteristics.characteristic: organism
characteristics.text: Escherichia coli
characteristics.characteristic: organism
characteristics.text: low-level McC production from plasmid
characteristics.characteristic: phenotype
characteristics.text: Escherichia coli
characteristics.characteristic: scientific_name
characteristics.text: Nissle 1917
characteristics.characteristic: strain
characteristics.text: EcN_Low_Rep1
characteristics.characteristic: title