characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-12T00:19:19Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-12T00:19:19Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112772504
characteristics.characteristic: External Id
characteristics.text: IFEVA (CONICET-UBA) Facultad de Agronomia Universidad de Buenos Aires (UBA)
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-12T00:19:19Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-12T00:19:19Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14773303
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12771:GTHA
characteristics.characteristic: Submitter Id
characteristics.text: 42
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: potato
characteristics.characteristic: common name
characteristics.text: Spunta
characteristics.characteristic: cultivar
characteristics.text: Protocols: Samples were harvested from 42 day-old plants at the beginning of the day. Three biological replicates, each consisting of a pool of leaves from six plants (the third completely expanded leaf), were collected. S. tuberosum var. Spunta wild type plants were used. Sprouted tubers of similar size were planted in pots of 1000 cm3. Plants were grown in a greenhouse with natural radiation (PAR = 500 mmol m-2 s-1) with temperatures ranging between 20°C and 24°C. Experiments were carried out between March and October with a photoperiod of about 10.5 h of light. *For shade avoidance experiments, plants were grown in a greenhouse with natural radiation for 28 days. Then, plants were transferred to simulated shade provided by the same light sources in combination with two green acetate filters (no. 089; LEE Filters, Hampshire, UK) to reduce blue and red light and the R:FR (red to far red) ratio, or kept in control conditions, for additional 14 days. *For drought stress treatment, plants were watered daily at field capacity for 28 days. Then, water was restricted to 35% of field capacity for 14 days. Total RNA was extracted using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich), according to the manufacturer’s instructions. Unstranded libraries were prepared using standard Illumina kit
characteristics.characteristic: description
characteristics.text: sporophyte vegetative stage
characteristics.characteristic: developmental stage
characteristics.text: drought environment
characteristics.characteristic: environmental stress
characteristics.text: BBX21-OE CH13
characteristics.characteristic: genotype
characteristics.text: green house study
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Solanum tuberosum
characteristics.characteristic: organism
characteristics.text: Solanum tuberosum
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.characteristic: organism part
characteristics.text: third expanded leaf
characteristics.characteristic: sampling site
characteristics.text: Solanum tuberosum
characteristics.characteristic: scientific_name
characteristics.text: GTHA
characteristics.characteristic: title