characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-12T00:19:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-12T00:19:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112328265
characteristics.characteristic: External Id
characteristics.text: Martin-Luther-University Halle-Wittenberg
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-12T00:19:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-12T00:19:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14439180
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12485:VH4-34_only_Bcells
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: B cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood by density-gradient centrifugation using Biocoll separation solution (Biochrom AG, Berlin, Germany). PBMCs were sorted for live cells or for IGHV4-34 and non-IGHV4-34 B cells using an anti-IGHV4-34 antibody (9g4, IgmBiosciences, Mountain View, California, USA). Genomic DNA of PBMCs was isolated using the GenElute mammalian genomic DNA miniprep kit (Sigma-Aldrich, Taufkirchen, Germany) according to the manufacturer’s instructions. Chromium Single Cell 5' Library Construction Kit
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: IGHV4-34 positive
characteristics.characteristic: immunophenotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: VH4-34_only_Bcells
characteristics.characteristic: title