characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-08-24T00:22:18Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-08-24T00:22:18Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110744408
characteristics.characteristic: External Id
characteristics.text: Centre for Genetic Manipulation of Crop Plants, University of Delhi South Campus, Prof. Deepak Pental and Dr. Paritosh Kumar
characteristics.characteristic: INSDC center name
characteristics.text: 2023-08-24T00:22:18Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-08-24T00:22:18Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12841933
characteristics.characteristic: SRA accession
characteristics.text: SAMIN0000052-Genome sequencing and assembly of B. Carinata HC20
characteristics.characteristic: Submitter Id
characteristics.text: 15 days
characteristics.characteristic: age
characteristics.text: IBDC
characteristics.characteristic: broker name
characteristics.characteristic: common name
characteristics.text: Homozygous plants of B. carinata accession HC20 were used for genome sequencing experiments. The HC20 accession was maintained by self-pollination for several generations and was mostly homozygous. For genome sequencing experiments, seedlings were grown in a growth chamber at 8 h light, 25 degree Celsius and 16 h dark, 10 degree Celsius cycle. Leaves were harvested from 15-days-old seedlings and immediately snap-frozen in liquid nitrogen and used for DNA extraction.
characteristics.characteristic: description
characteristics.text: Seedling stage
characteristics.characteristic: dev_stage
characteristics.text: HC20
characteristics.characteristic: genotype
characteristics.text: Brassica carinata
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.characteristic: organism part
characteristics.text: Tetraploid-BBCC
characteristics.characteristic: ploidy
characteristics.text: Brassica carinata
characteristics.characteristic: scientific_name
characteristics.text: Genome sequencing and assembly of B. Carinata HC20
characteristics.characteristic: title