characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-16T07:00:06Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-16T07:00:06Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112156246
characteristics.characteristic: External Id
characteristics.text: Department of Microbiology and Molecular GeneticsIMRIC, Faculty of Medicine, The Hebrew University of Jerusalem
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-16T07:00:06Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-16T07:00:06Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14266143
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12429:Sample 9
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Cultures ofE. coliMG1655 were grown at 37°C in either rich LB medium or minimal E medium pH 7.019supplied with 0.4% glucose (EG medium). At OD600of ~0.4the cells were collected. Total RNA was extracted, using TRI-reagent RNAtag-seq libraries were constructed as described in (Melamed et al., 2018). The cDNA was cleaned-uptwice with 1.5 volumes of Ampure XP (Beckman-Coulter) and amplified by 9 cycles of PCR.
characteristics.characteristic: description
characteristics.text: exponential phase
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: minimal E medium
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Escherichia coli str. K-12 substr. MG1655
characteristics.characteristic: organism
characteristics.text: Escherichia coli str. K-12 substr. MG1655
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Escherichia coli str. K-12 substr. MG1655
characteristics.characteristic: scientific_name
characteristics.text: Sample 9
characteristics.characteristic: title