characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-31T00:18:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-31T00:18:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112248531
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF MANCHESTER
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-31T00:18:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-31T00:18:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14359396
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12384:Sample 3
characteristics.characteristic: Submitter Id
characteristics.text: 4
characteristics.unit: month
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Male eight-week-old C57Bl/6N mice underwent transverse aortic constriction (TAC) or sham operations. Mice were terminated eight weeks post-surgery or when HF symptoms developed, and atrioventricular node (AV node) biopsies were collected. AV node biopsies taken from three mice were pooled and three such replicates were obtained from HF (TAC) and control (sham-operated) mice. Total RNA was isolated from the AV node biopsies using the RNeasy Micro kit (Qiagen) according to the manufacturer’s instructions. Total RNA was submitted to the Genomic Technologies Core Facility (University of Manchester). RNA quantity and integrity were measured using a 2200 TapeStation (Agilent Technologies). TrueSeq Stranded mRNA assay (Illumina) was used to generate libraries.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: 3
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: atrioventricular node
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6N
characteristics.characteristic: strain
characteristics.text: Sample 3
characteristics.characteristic: title