characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-08-13T00:19:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-08-13T00:19:08Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111376253
characteristics.characteristic: External Id
characteristics.text: Gynecologic Cancer Center, Department of Radiation Oncology, The Ajou University of School of Medicine, Suwon 16499, Korea
characteristics.characteristic: INSDC center name
characteristics.text: 2023-08-13T00:19:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-08-13T00:19:08Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13473244
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12187:Sample 21
characteristics.characteristic: Submitter Id
characteristics.text: 44
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.characteristic: clinical information
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Exosome was isolated from human plasma by mixing the plasma with Exo2D RNA solution (Exosomeplus). Exosomal RNA from plasma-derived exosomes was extracted using the miRNeasy Serum/Plasma Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions.The extracted RNA concentration was calculated by Quant-IT RiboGreen(Invitrogen). RNA size was confirmed using Agilent RNA 6000 Pico Kit and Small RNA Kit on Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). The 10ng of RNA isolated from each sample was used to construct sequencing libraries with the SMARTer smRNA-Seq Kit for Illumina, following the manufacturer's protocol. Briefly, Input RNA is first polyadenylated in order to provide a priming sequence for an oligo(dT) primer. cDNA synthesis is primed by the 3’ smRNA dT Primer, which incorporates an adapter sequence at the 5’end of each RNA template, it adds non templated nucleotides which are bound by the SMRT smRNA Oligo-enhanced with locked nucleic acid (LNA) technology for greater sensitivity. In the template-switching step, PrimeScript RT uses the SMART smRNA Oligo as a template for the addition of a second adapter sequence to the 3’end of each first-strand cDNA molecule. In the next step, full-length Illumina adapters (including index sequences for sample multiplexing) are added during PCR amplification. The Forward PCR Primer binds to the sequence added by the SMART smRNA Oligo, while the Reverse PCR Primer binds to the sequence added by the 3’ smRNA dT Primer. The amplified libraries were purified from 6% Novex TBE-PAGE gels (Thermo Fisher, MA) to excise over than 138 bp (over than 18 bp of cDNA plus 120 bp of adaptors) fractionResulting library cDNA molecules include sequences required for clustering on an Illumina flow cell.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: cervical cancer
characteristics.characteristic: disease
characteristics.text: 3C2
characteristics.characteristic: disease staging
characteristics.text: exosome
characteristics.characteristic: fraction
characteristics.text: AU-20-11
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood plasma
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: Sample 21
characteristics.characteristic: title