characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-12T00:19:24Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-12T00:19:24Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113399760
characteristics.characteristic: External Id
characteristics.text: Berlin Institute for Medical Systems Biology, Max Delbruck Center for Molecular Medicine, Berlin, Germany
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-12T00:19:24Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-12T00:19:24Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15394907
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12886:Neurite_scr_3
characteristics.characteristic: Submitter Id
characteristics.text: 0
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: neuron of crebral cortex
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Primary cortical neurons were prepared from P0 mouse pups (C57B/6), and cultured as previously described (Kaech, 2006). 106 primary neurons were plated per a Millicell cell culture insert (6-well, Millipore MCRP06H48), coated with poly-D-Lysine and laminin. Infection with lentiviral particles was at DIV5 and cells were harvested 72 hours later (DIV9). Separation into soma and neurites was performed using a cotton swab as described (Zappulo, 2017) and further separation of soma on nuclear and cytoplasmic fractions was performed using NE-PER Nuclear and Cytoplasmic Extraction Kit (Thermo 78833) following manufacturer’s instructions. RNA was extracted from Trifast using 2-step chloroform extraction and isopropanol precipitation. RNA samples were quantified using qubit and analysed for integrity with tapestation. Good separation was verified by RT-qPCR. TrueSeq stranded mRNA Library kit; 1ul of 1:300 ERCC1 spike-in was added to 49ul initial solution with 150ng total RNA.; Final PCR was done with 12-16 cycles’ reaction
characteristics.characteristic: description
characteristics.text: neonate
characteristics.characteristic: developmental stage
characteristics.text: neurite
characteristics.characteristic: fraction
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: brain
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: mixed
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: Neurite_scr_3
characteristics.characteristic: title