characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-29T00:17:22Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-29T00:17:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112328429
characteristics.characteristic: External Id
characteristics.text: Department of Biomedical Sciences, University of Copenhagen, Denmark
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-29T00:17:22Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-29T00:17:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14439344
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12517:Sample 10
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: H9
characteristics.characteristic: cell_line
characteristics.text: embryonic stem cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: The mesoderm differentiation protocol used in this dataset is a combination of published protocols that were further modified. These will be detailed in the corresponding publication After medium removal, cells were washed with PBS and scraped off in RLT buffer (QIAGEN RNeasy kit). QIAGEN RNeasy kit. Library was constructed with NEBNext Ultra II RNA library Prep Kit (New England BioLabs) using 120 ng of RNA. 9 cycles of PCR amplification were performed, and AMPure XP (Beckman Coulter) was used for cleanup. The quality was assessed using Fragment Analyzer (Agilent) and the concentration of cDNAs was measured using Qubit Fluorometer (Invitrogen).
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: inner cell mass
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 10
characteristics.characteristic: title