characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-17T11:00:11Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-17T11:00:11Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110278445
characteristics.characteristic: External Id
characteristics.text: Department of Cell and Molecular Biology Karolinska Institutet
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-17T11:00:11Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-17T11:00:11Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12376059
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11920:Sample 4
characteristics.characteristic: Submitter Id
characteristics.text: embryonic day 14.5
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Dorsal skin of mouse embryos (C57BL/6J - E12.5, E13.5, and E14.5) was dissected with the help of fine dissection tools and dissected skins were incubated in Dispase II (2mg/ml), Collagenase IA (0.2%), and DNAse I (20U/ul) in PBS for 40 minutes at 37°C in ultra-low attachment plates (Corning Costar) on an orbital shaker. The obtained cell suspension was passed through a 40 mm cell strainer. The flow-through was spun down, and subsequently resuspended in PBS + 0.04% BSA. Samples were transported to core facility in PBS + 0.04% BSA in Eppendorf tubes that had been coated with PBS + 20% BSA overnight. Viability of the cell suspension was determined using trypan blue on an EVE automatic cell counter. Nucleic acid extraction was performed using the 10X Genomics Chromium Single Cell 3’ kit v2 according to the manufacturer’s instruction. Single-cell cDNA libraries were prepared using the 10X Genomics Chromium Single Cell 3’ kit v2 according to the manufacturer’s instruction.
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: 10x_17_036
characteristics.characteristic: id at core facility
characteristics.text: E14.5 Replicate 2
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: dorsal skin
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: Sample 4
characteristics.characteristic: title