characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-20T00:19:31Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-20T00:19:31Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113542855
characteristics.characteristic: External Id
characteristics.text: Mario Negri Institute for Pharmacological Research (IRCCS)
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-20T00:19:31Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-20T00:19:31Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15539272
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12990:VCaP_shMAT2A_Input_REP3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: VCap
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cell line samples (3x106 cells per IP) were exposed to formaldehyde (37%) to cross-link protein-DNA complexes and processed as previously described (Albino et al., 2012; Longoni et al., 2013). Chromatin extracts were immunoprecipitated with antibodies against AR (cat. 06-680, Millipore), H3K4me2 (cat. 9725S, Cell Signaling) and IgG (Millipore) as control. Quantitative real-time PCR was performed using KAPA SYBR Fast qPCR kit (KAPA Biosystems) and primers spanning the region of interest (Table 3). The amount of immunoprecipitated DNA was calculated in reference to a standard curve and normalized to input DNA or subsequently to IgG (Longoni et al., 2013). For comparison of test and control samples, the amount of DNA was then normalized to the control samples.
characteristics.characteristic: description
characteristics.text: prostate cancer
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: prostate gland
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: VCaP_shMAT2A_Input_REP3
characteristics.characteristic: title