characteristics.text: ERC000023
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-08-22T00:27:17Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-08-22T00:27:17Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112329348
characteristics.characteristic: External Id
characteristics.text: NETHERLANDS INSTITUTE OF ECOLOGY
characteristics.characteristic: INSDC center name
characteristics.text: 2023-08-22T00:27:17Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-08-22T00:27:17Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14440258
characteristics.characteristic: SRA accession
characteristics.text: Inoculum-AGS-16S
characteristics.characteristic: Submitter Id
characteristics.text: 0.015
characteristics.unit: L
characteristics.characteristic: amount or size of sample collected
characteristics.text: Sequencing batch reactor
characteristics.characteristic: broad-scale environmental context
characteristics.text: 2020-11-23
characteristics.characteristic: collection date
characteristics.text: Aerobic granular sludge adapted to experimental conditions
characteristics.characteristic: description
characteristics.text: Synthetic wastewater
characteristics.characteristic: environmental medium
characteristics.text: Netherlands
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 51.98699968
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 5.67116169
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: Gelderland
characteristics.characteristic: geographic location (region and locality)
characteristics.text: Granulated biomass, aerobic granular sludge
characteristics.characteristic: local environmental context
characteristics.text: PCR
characteristics.characteristic: nucleic acid amplification
characteristics.text: Qiagen DNAeasy Power Soil Pro
characteristics.characteristic: nucleic acid extraction
characteristics.text: 3
characteristics.characteristic: number of replicons
characteristics.text: activated sludge metagenome
characteristics.characteristic: organism
characteristics.text: aerobic
characteristics.characteristic: oxygenation status of sample
characteristics.text: 7.25
characteristics.characteristic: pH
characteristics.text: 341F-805R
characteristics.characteristic: pcr primers
characteristics.text: Local Treatment of Urban sewage and Streams for Healthy Reuse (LOTUSHR) - www.lotushr.org
characteristics.characteristic: project name
characteristics.text: Sequencing batch reactor
characteristics.characteristic: reactor type
characteristics.text: 15mL of harvested photogranules were homogenised by a glass/Teflon tissue grinder, aliquoted into five 2 mL Eppendor tubes, centrifuged at 14.870 rcf for 10 min and the supernatant discarded, the cell pellets were immediately frozen at -80 �C until further processing.
characteristics.characteristic: sample material processing
characteristics.text: 0.2
characteristics.unit: g
characteristics.characteristic: sample volume or weight for DNA extraction
characteristics.text: activated sludge metagenome
characteristics.characteristic: scientific_name
characteristics.text: Illumnia MiSeq
characteristics.characteristic: sequencing method
characteristics.text: 14
characteristics.unit: days
characteristics.characteristic: sludge retention time
characteristics.text: 16S rRNA gene
characteristics.characteristic: target gene
characteristics.text: Aerobic granular sludge
characteristics.characteristic: title
characteristics.text: Synthetic wastewater
characteristics.characteristic: wastewater type