characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-03T00:17:40Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-03T00:17:40Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111529197
characteristics.characteristic: External Id
characteristics.text: NTU
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-03T00:17:40Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-03T00:17:40Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13639178
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12343:5 ±PJ34
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: C2C12
characteristics.characteristic: cell_line
characteristics.text: myoblast
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Collected using Trizol The murine muscle myoblast cell line C2C12 were purchased (ATCC, VA, USA) and maintained in proliferation media, composed of Dulbecco’s Modified Eagle’s Medium (DMEM) 25 mM glucose (Lonza, UK) supplemented with 10% (v/v) fetal bovine serum (FBS) (Thermo, UK) and 1% Penicillin/Streptomycin (P/S) (Thermo, UK). When cells reached 70 – 80% confluence, differentiation medium, composed of DMEM 25 mM glucose supplemented with 2% horse serum (HS) (Thermo, UK) and 1% P/S, was added to induce differentiation. Myoblasts were treated with either vehicle controls or the PARP inhibitor and PJ34 (MedChemExpress, NJ, USA) at working concentrations of 10 µM. Dexamethasone treatment conc was 1uM. Phenol-chlorofom Etoh precipitation - Column clean up NOVOGENE
characteristics.characteristic: description
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: skeletal muscle tissue
characteristics.characteristic: organism part
characteristics.text: 2
characteristics.characteristic: replicate
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: 5 ±PJ34
characteristics.characteristic: title