characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-28T00:31:42Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-28T00:31:42Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112327707
characteristics.characteristic: External Id
characteristics.text: Martin-Luther-University Halle-Wittenberg
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-28T00:31:42Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-28T00:31:42Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14438622
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12491:HA20-14
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: mononuclear cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Peripheral blood of patients with germline TNFAIP3 mutations and healthy control individuals was collected. Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood by density-gradient centrifugation using Biocoll separation solution. Genomic DNA of PBMCs and lymphoma tissue was isolated using the GenElute mammalian genomic DNA miniprep kit The rearranged V, D and J gene segments of the IGH and TRB loci were amplified in a multiplex PCR using BIOMED2-FR1 (IGH) or -TRB-B primer pools together and 250 ng of genomic DNA. The primers were purchased from Metabion International AG. As described in17, two consecutive PCR reactions were performed to generate IGH and TRB fragments tagged with Illumina-compatible adapters.
characteristics.characteristic: description
characteristics.characteristic: developmental stage
characteristics.text: A20 haploinsufficiency
characteristics.characteristic: disease
characteristics.text: HA20-14
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: peripheral blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.characteristic: sex
characteristics.text: HA20-14
characteristics.characteristic: title