characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-20T00:18:59Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-20T00:18:59Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110418563
characteristics.characteristic: External Id
characteristics.text: University Medical Center Gottingen Department of Gynecology and Obstetrics Molecular Gynecology
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-20T00:18:59Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-20T00:18:59Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12516917
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12000:HCC1806 siRNF40.2_H3K79me3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HCC1806
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: HCC1806 cells were grown in RPMI GlutaMax medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37 C and 5% CO2 siRNA transfections were performed using Lipofectamine RNAiMAX(Invitrogen) according to the manufacturer's instructions Chromatin immunoprecipitation was performed 48 hrs after transfection using antibodies against H2Bub1 (Cat. No. 5546 S, Cell Signaling Technology), H3K79me3 (Cat. No. C15410068, Diagenode), H3K27ac (Cat. No. C15410196, Diagenode). Next-generation sequencing library was prepared using the KAPA Hyper Prep Kit (KR0961–v6.17) according to the manufacturer’s instructions and samples were sequenced (single-end 50 bp).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: triple-negative breast cancer
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: mammary gland
characteristics.characteristic: organism part
characteristics.text: 2
characteristics.characteristic: replicate
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: HCC1806 siRNF40.2_H3K79me3
characteristics.characteristic: title