characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-08-01T00:17:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-08-01T00:17:08Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113595581
characteristics.characteristic: External Id
characteristics.text: National Cancer Center, Republic of Korea
characteristics.characteristic: INSDC center name
characteristics.text: 2023-08-01T00:17:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-08-01T00:17:08Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15589941
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13053:sample_3H3
characteristics.characteristic: Submitter Id
characteristics.text: 6
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: B6 mice were intravenously injected with 5 × 10^5 pme-1 Thy1.1+CD8+ T cells. Subsequently, they were immunized with mgp100 peptide emulsified in IFA. At day 0 and 2, the mice were intraperitoneally administered with 100 μg of rat IgG, 3H3, or 3H3 plus TKS-1. After five days from the cell transfer, inguinal lymph nodes (LNs) were collected from each group of mice. Thy1.1+ cells were then isolated from the collected LNs using Thy1.1-microbeads (Miltenyi Biotec). RNA was extracted from the pmel-1 Thy1.1+CD8+ T cells using Trizol reagent (Invitrogen). Library was constructed using TruSeq Stranded mRNA Sample Prep Kit according to manufacturers protocol.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: lymph node
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: 3H3
characteristics.characteristic: stimulus
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: sample_3H3
characteristics.characteristic: title