characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-07-20T00:18:54Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-07-20T00:18:54Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110177403
characteristics.characteristic: External Id
characteristics.text: University Medical Center Gottingen Department of Gynecology and Obstetrics Molecular Gynecology
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-20T00:18:54Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-07-20T00:18:54Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12276211
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11860:HCC1806 siRNF40.2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HCC1806
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: HCC1806 cells were grown in RPMI GlutaMax medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. siRNA transfections were performed using Lipofectamine® RNAiMAX (Invitrogen) according to the manufacturer’s instructions. RNA was extracted usiung QIAzol® Lysis Reagent (Qiagen) according to the manufacturer's protocol. Libraries were prepared using NEXTflex™ Rapid Illumina Directional RNA-Seq Library Prep Kit (Illumina®) after verifying the RNA integrity on an agarose gel. Library quality was assessed using an Agilent Bioanalyzer 2100.
characteristics.characteristic: description
characteristics.text: triple-negative breast cancer
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: mammary gland
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: HCC1806 siRNF40.2
characteristics.characteristic: title