characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-06-28T00:17:09Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-06-28T00:17:09Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110278101
characteristics.characteristic: External Id
characteristics.text: European Research Institute for the Biology of Ageing University Medical Center Groningen
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-28T00:17:09Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-06-28T00:17:09Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12375715
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11914:USP13 wt empty vector
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: USP-13-Med
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Half a million cells were plated on six well plates. After 48 hours, cells were harvested using Tryple. Pelleted cells were washed with 1xPBS before being stored as dry pellets at -80 degree Celsius. For whole transcriptome analysis, RNA was isolated using the RNeasy mini kit (Qiagen) in accordance to manufacturer's protocol and RNA quality was examined using the Agilent 2100 Bioanalyzer (Agilent Technologies). 250-500 ng of total RNA was used as input for mRNA enrichment using NEXTflex Poly(A) Beads (Bio Scientific) followed by library preparation using a NEXTflex Rapid Directional qRNA-Seq Kit (Bio Scientific).
characteristics.characteristic: description
characteristics.text: juvenile stage
characteristics.characteristic: developmental stage
characteristics.text: medulloblastoma
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: mixed brain and cerebellum
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: USP13 wt empty vector
characteristics.characteristic: title