characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-10-16T10:00:07Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-16T10:00:07Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111491264
characteristics.characteristic: External Id
characteristics.text: Institute of Molecular Genetics of the Czech Academy of Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2023-10-16T10:00:07Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-16T10:00:07Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13585278
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12310:HSC + PBS
characteristics.characteristic: Submitter Id
characteristics.text: 12
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: hematopoietic stem cell
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mice were treated intraperitoneally with 35 ug of LPS (lipopolysacharide) or PBS and sacrificed 4 hours after the treatment. Tibia, femur and hip bones were isolated from both hind legs and cleaned from tissue. Cells were isolated by crunching the BM with pestle and mortar and filtered through nylon mesh. The cell suspension was treated with ACK to remove erythrocytes. Next, the BM cells were stained with mix of lineage antibodies (CD3e, B220, Ter119, Gr-1 and CD11b) and separated on autoMACS separator to enrich for immature populations. The sorted unstained fraction was further stained with c-Kit, Sca-1, CD48 and CD150 antibodies and sorted on BDinflux machine. In total, 50000 cells were pooled for each experimental condition (HSC+PBS, HSC+LPS). WT C57BL6 mice were treated intraperitoneally with 35 ug of LPS from Escherichia coli 0111:B4 (InvivoGen, San Diego, CA, USA) or PBS control. Accessible DNA was extracted during library preparation with ATAC seq kit (Active Motif Carlsbad, CA, USA). Sequencing libraries were prepared using ATAC seq kit (Active Motif Carlsbad, CA, USA) according to the manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: A
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: bone marrow
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: HSC + PBS
characteristics.characteristic: title