characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-10-02T00:19:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-02T00:19:25Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112240182
characteristics.characteristic: External Id
characteristics.text: OXFORD
characteristics.characteristic: INSDC center name
characteristics.text: 2023-10-02T00:19:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-02T00:19:25Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14351063
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12312_2:FS3_BASAL
characteristics.characteristic: Submitter Id
characteristics.text: 49
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: fibroblast
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Capsular fibroblasts were isolated from frozen shoulder patients and were co-cultured with monocyte-derived macrophages(MDM), which were isolated from blood cones and treated with M-CSF for 5 days. Capsule-derived fibroblasts isolated from frozen shoulder patients were added to MerTKhigh or MerTKlow incubations at a density of 200,000 cells/well. Fibroblasts and MDMs were co-cultured for 48 hrs in complete media containing 3% FCS prior to harvest and sorting. RNA was extracted using RNEasy microprep kit (Qiagen) according to the manufacturers protocol. Double stranded cDNA library construction was performed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L) with custom adapters and dual indexed barcodes.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: frozen shoulder
characteristics.characteristic: disease
characteristics.text: FS3
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: articular capsule of glenohumeral joint
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: FS3_BASAL
characteristics.characteristic: title