characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-11-14T17:08:32Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-11-14T17:08:32Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112194794
characteristics.characteristic: External Id
characteristics.text: Affiliated Hospital of Zunyi Medical University
characteristics.characteristic: INSDC center name
characteristics.text: 2023-11-14T17:08:32Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-11-14T17:08:32Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14305871
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12445:Sample 2
characteristics.characteristic: Submitter Id
characteristics.text: 55
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Patients and tissue collection. for HC, presence of a normal liver (no steatosis or cirrhosis) on ultrasound evidence; for NAFLD was based on ultrasound evidence of fatty liver in the absence of other (viral, alcohol, metabolic) causes. Liver tissue was collected by a wedge liver biopsy from free edge of the liver lobe during laparoscopic cholecystectomy. The study protocol received institutional review board (IRB) approval before the beginning of the study, in accordance with The Code of Ethics of the Declaration of Helsinki, and informed consent was obtained from all patients. tissue samples were immediately stored in RNA-later for 24 hours at 4℃, and subsequently at -80℃. RNA isolation and quality controls. Approximately 10-20 mg of liver tissues were homogenized in 1 mL Trizol (Thermo Fisher Scientific, shanghai, China) on ice, incubated for 5 min, then added 200 μL chloroform. After low-temperature centrifugation, 500 μL isopropanol was used to precipitate RNA. The precipitate was washed with 75% ethanol (DEPC water configuration), and then dissolved in 30 μL DEPC water (shanghai Generay Biotech Co., Ltd, shanghai, China) after evaporating all ethanol. The RNA concentration, purity and integrity were measured by Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Beijing, China). RNA concentration, purity and RNA integrity number (RIN) were measured using an Agilent 2100 Bioanalyzer before sequencing. 2 μg of RNA meeting the requirements of RIN ≥ 7 and 28S/18S ≥ 1.0 were taken for library construction.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: nonalcoholic fatty liver disease
characteristics.characteristic: disease
characteristics.text: NAFLD2
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: Sample 2
characteristics.characteristic: title