characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-10-31T00:26:55Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-31T00:26:55Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113464341
characteristics.characteristic: External Id
characteristics.text: Instituto de Tecnologia Quimica e Biologica Antonio Xavier - Universidade Nova de Lisboa (ITQB NOVA)
characteristics.characteristic: INSDC center name
characteristics.text: 2023-10-31T00:26:55Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-31T00:26:55Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15460107
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12956:C2
characteristics.characteristic: Submitter Id
characteristics.text: 14
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: 14-day-old seedlings were treated 3 mg/mL of COM in 0.5 % DMSO in MiliQ water or the corresponding mock solution were added to each well. Seedlings were treated for 30 min, a total of two seedlings were collected and instantly frozen in liquid nitrogen. Arabidopsis thaliana Col-0 seedlings were germinated on solid MS media and after germination in liquid 1/2 MS media on an Aralab Fitoclima climate chamber with 150 μmol·s-1·m-2 light intensity, following a 10 h/14 h day/night cycle, under constant temperature of 20 °C and 60 % humidity. Total RNA was extracted at 4 ºC from two ground seedlings by addition of TRI reagent and chloroform, precipitated with isopropanol and washed with 70 % ethanol. Samples were subjected to DNase treatment using a TURBO DNA-free Kit (Ambion). Quantification and quality assessment of all RNA samples were evaluated on a TapeStation (Agilent). Libraries were generated at BGI according to the DNBSEQ stranded mRNA library system.
characteristics.characteristic: description
characteristics.text: seedling development stage
characteristics.characteristic: developmental stage
characteristics.text: Col-0
characteristics.characteristic: ecotype
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: whole plant
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: C2
characteristics.characteristic: title