characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-21T10:00:07Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-21T10:00:07Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110375393
characteristics.characteristic: External Id
characteristics.text: Institute of Molecular Genetics of the Czech Academy of Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-21T10:00:07Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-21T10:00:07Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12473589
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11967:MCAO
characteristics.characteristic: Submitter Id
characteristics.text: 50 to 56
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Three days after surgery, we isolated the cerebral cortex adjacent to the site of injury. Fluorescence-activated cell sorting was used to obtain CD31/45 negative and CellTrace-calcein green positive viable cells carrying tdTomato/NG2 red fluorescence. In adult males, middle cerebral artery occlusion (MCAO) induced focal cerebral ischemia. For healthy tissue (CTRL), sham-operated mice were used. Software cellranger (10X Genomics) version 6.1.1 was used to demultiplex fastq files from Illumina bcl files with default parameters. First-strand cDNA was extracted from cell suspension (8 individuals pooled per group) during library preparation routine using Chromium next gem single-cell 3′ reagent kit (version 3.1). Single-cell RNA-seq libraries were prepared using the Chromium controller instrument and Chromium next gem single-cell 3′ reagent kit (version 3.1) according to the manufacturer’s protocol (both 10× Genomics, Pleasanton, CA, USA) targeting at 4000 cells per sample. The quality and quantity of the resulting cDNA libraries was determined using Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Cerebral ischemia
characteristics.characteristic: disease
characteristics.text: Rosa26-tdTomato/Cspg4-CreERT2
characteristics.characteristic: genotype
characteristics.text: tdTomato-positive; NG2-positive; CD31-negative; CD45-negative
characteristics.characteristic: immunophenotype
characteristics.text: pool of injured individuals
characteristics.characteristic: individual
characteristics.text: middle cerebral artery occlusion
characteristics.characteristic: injury
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: cerebral cortex
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: MCAO
characteristics.characteristic: title