characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-01T05:28:43Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-01T05:28:43Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113424943
characteristics.characteristic: External Id
characteristics.text: Center for Omics Sciences IRCCS San Raffaele Scientific Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-01T05:28:43Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-01T05:28:43Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15420071
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12946:shPINK1_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HCT116
characteristics.characteristic: cell_line
characteristics.text: colorectal cancer cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were trypsinized, harvested and washed with PBS 1%. Then, the pellet was immediately used for RNA extraction or frozen and stocked at -80°C until use. HCT116 cells were grown in DMEM supplemented with 10% Fetal Bovine Serum. About 1.2e6 HCT116 cells were plated on 6 cm plates. The day after cells were overnight infected with SCR or shPINK1 constructs. After 24h, Puromycin was added at 2ug/ml concentration for each plate. After three days of selection, cells were harvested for RNA extraction and sequencing. RNA extraction was performed using the RNeasy kit (74106) from Qiagen. Ribo minus total RNA stranded kit was used.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: colorectal carcinoma
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: intestine
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: shPINK1_1
characteristics.characteristic: title