characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-17T00:19:25Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-17T00:19:25Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10113932
characteristics.characteristic: External Id
characteristics.text: Cancer Biomarker Centre, CRUK Manchester Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-17T00:19:25Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-17T00:19:25Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7770486
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10950:CellLineVM_batch2_CDX19_NNE_M
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: CDX derived cell line
characteristics.characteristic: cell_line
characteristics.text: non-neuroendocrine
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Circulating tumour cells were collected from patient blood using RosetteSep (Stemcell Technologies) and injected into the flank of non-obese diabetic severe combined immunodeficient interleukin-2 receptor γ–deficient mice. Tumours were excised from terminally sacrificed mice, dissociated into single cells using Miltenyi Biotec’s tumour dissociation kit (#130-095-929) following the manufacturer’s instructions on a gentleMACS octo dissociator (Miltenyi Biotec, #130-096-427) and cultured ex vivo. Suspension and adherent cells were separated, cultured on either plastic or Matrigel for 24 hours and total RNA was harvested from cells using a RNeasy Mini Kit (Qiagen, #74106) according to the manufacturer’s instructions. Total RNA was harvested from cells using a RNeasy Mini Kit (Qiagen, #74106) according to the manufacturer’s instructions using ice cold RLT buffer with β-mercaptoethanol (Sigma-Aldrich, #M6250). On-plate lysis was performed for adherent cells whereas suspension cells were lysed as a cell pellet. For cell numbers >1 x 106, the lysed cells were homogenised using a QIAshredder column (Qiagen, #79654) and for cell numbers <1 x 106 cells were homogenised by vortexing. RNA was measured using a NanoDrop (Thermo Fisher Scientific) and stored at -80°C. Indexed PolyA libraries were prepared using 200 ng of total RNA with the Agilent SureSelect Strand Specific RNA Library Prep Kit for Illumina Sequencing (Agilent, #G9691B).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: small cell lung carcinoma
characteristics.characteristic: disease
characteristics.text: Matrigel
characteristics.characteristic: growth condition
characteristics.text: CDX17P
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: blood
characteristics.characteristic: metastatic site
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: lung
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 2
characteristics.characteristic: sequencing batch
characteristics.text: CellLineVM_batch2_CDX19_NNE_M
characteristics.characteristic: title