characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-10-31T00:26:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-31T00:26:36Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111512972
characteristics.characteristic: External Id
characteristics.text: Department of Chemical Engineering and Biotechnology - University of Cambridge
characteristics.characteristic: INSDC center name
characteristics.text: 2023-10-31T00:26:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-31T00:26:36Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13623953
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12337:SM3C
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Cultures of E. coli BL21 (DE3) - control and evolved - were harvested in late-log phase phase, after 8 hours of growth. 2 x 10^9 cells were collected for processing. Each bacterial culture was treated with the RNAprotect reagent (Qiagen). Total RNA from E. coli BL21 (DE3) samples was extracted using the ‘RNeasy Protect Bacteria Mini Kit’ (RNAprotect Tissue Reagent) according to the manufacturer’s instructions for protocol 4: ‘Enzymatic Lysis and Proteinase K Digestion of Bacteria’. For this protocol proteinase K (Qiagen) and DNAse I (Qiagen) were purchased separately and used according to the manufacturer’s instructions in protocol 4. The sequencing library was constructed from 500ng per sample of isolated total RNA and processed using the NEBNext® rRNA Depletion Kit (Bacteria) - E7850, and the KAPA RNA HyperPrep Kit (Roche p/n KK8540) for creating the library according to manufacturer’s instructions.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: terrific broth media pH7
characteristics.characteristic: growth condition
characteristics.text: Escherichia coli BL21(DE3)
characteristics.characteristic: organism
characteristics.text: Escherichia coli BL21(DE3)
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: wild type phenotype
characteristics.characteristic: phenotype
characteristics.text: Escherichia coli BL21(DE3)
characteristics.characteristic: scientific_name
characteristics.text: BL21 (DE3)
characteristics.characteristic: strain
characteristics.text: SM3C
characteristics.characteristic: title