characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-11-16T17:00:04Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-11-16T17:00:04Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA114003521
characteristics.characteristic: External Id
characteristics.text: University of Bern, Interfaculty Bioinformatics Unit
characteristics.characteristic: INSDC center name
characteristics.text: 2023-11-16T17:00:04Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-11-16T17:00:04Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15987368
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13141:pd11
characteristics.characteristic: Submitter Id
characteristics.text: over 18
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Palatal connective tissue grafts (CTGs) from four groups 1) anterior deep (AD), 2) anterior superficial (AS), 3) posterior deep (PD), and 4) posterior superficial (PS) were obtained from 16 systemically and periodontally healthy subjects per group. The Ethics Committee, Bern, Switzerland (ethical code ID 2018-00661 from 13.08.2018) approved the study, and informed consent was obtained from all patients. The CTGs were biopsied during recession coverage surgeries performed by the same periodontist (Prof. Dr. Sculean). CTGs harvested in the region of lateral incisor, canine and premolars are designated as anterior (A), whereas CTGs harvested in the molars’ area are designated as posterior (P). At each location, the tissue sample was harvested in toto as a free gingival graft (~6x4 mm). Subsequently, the epithelial layer of ~0.5 mm was removed by sharp dissection using a C15 blade and the CTG was split into two thinner grafts – a superficial (S) located immediately subepithelially and a deep (D) located closer to the bone. Immediately after the excision, ~1/3 of each tissue sample was immediately placed in RNAlater (ThermoFisher Scientific, Basel, Switzerland) for 20 h before tissue homogenization using gentleMACS Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany) followed by RNA extraction using the RNeasy Fibrous Tissue Mini Kit (Qiagen, Basel, Switzerland). Quantity and quality of RNA extracted from each of the 64 collected CTGs was assessed using the Qubit RNA BR Assay Kit on a Qubit 4.0 fluorometer (ThermoFisher Scientific) as well as the Fragment Analyzer RNA Kit on an Advanced Analytical Fragment Analyzer System (Agilent Technologies, Basel, Switzerland). Sequencing libraries were produced using an illumina TruSeq Stranded mRNA Library Prep kit in combination with TruSeq RNA UD Indexes according to illumina’s guidelines (Illumina, San Diego, CA, USA).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: palate
characteristics.characteristic: organism part
characteristics.text: posterior deep palate
characteristics.characteristic: sampling site
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: not available
characteristics.characteristic: sex
characteristics.text: pd11
characteristics.characteristic: title