characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-29T00:22:39Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-29T00:22:39Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111468773
characteristics.characteristic: External Id
characteristics.text: KYUNG HEE UNIVERSITY
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-29T00:22:39Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-29T00:22:39Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13562803
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12295:Control1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Japanese rice
characteristics.characteristic: common name
characteristics.text: Dongjin
characteristics.characteristic: cultivar
characteristics.text: Protocols: Anthers at the bicellular and tricellular stages of stages 11-13 were collected. Seeds of japonica rice cv. Dongjin was sterilized with 50% of sodium hypochlorite and germinated in MSO media in an incubator (14-h light/10-h dark, 28 °C (day)/22 °C (night), humidity 80%). After 1 week of germination, seedlings were transferred and grown in the greenhouse for a period of 4 weeks under growth chamber conditions and transferred to the field. Mature anthers were frozen in LN2 and finely ground using mortar. Grounded samples were homogenized using TRI reagents (RNAiso, Takara) and BCP (1-bromo-3-chloropropane). DNA, RNA, and protein layers were separated by centrifugation. Then, total RNA was extracted using the RNeasy Mini Kit (QIAGEN). The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, \"tagmentation\" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified.
characteristics.characteristic: description
characteristics.text: mixed J bicellular and tricellular pollen stage
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: field study
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Oryza sativa Japonica Group
characteristics.characteristic: organism
characteristics.text: Oryza sativa Japonica Group
characteristics.characteristic: organism
characteristics.text: anther
characteristics.characteristic: organism part
characteristics.text: Oryza sativa Japonica Group
characteristics.characteristic: scientific_name
characteristics.text: Control1
characteristics.characteristic: title