characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-10-26T11:39:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-31T00:26:33Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111329690
characteristics.characteristic: External Id
characteristics.text: Ghent University Global Campus, Ghent University
characteristics.characteristic: INSDC center name
characteristics.text: 2022-10-26T11:39:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-31T00:26:33Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13427254
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12109:Pool
characteristics.characteristic: Submitter Id
characteristics.text: 12
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: splenocyte
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mice were infected by intraperitoneal (i.p) injection of with 200 parasites in 100 uL of Dulbecco's Phosphate Buffered Saline. Spleens were isolated at day 42 p.i. for T. evansi Merzouga 93. Single cell suspensions were prepared by homogenizing spleen in 6 mL of DMEM supplemented with 10% FBS and 1% penicillin/streptomycin using gentleMACS Dissociator. Cells were spun down at 314 x G for 7 minutes at 4oC after being filtered through a 70 µm pore size cell strainer. Red blood cells were removed using RBC Lysis Buffer from Biolegend. Spleen cells suspension from a single mouse and pooled spleen cells suspension from 3 individual mice (1:1:1 ratio) were prepared for further protocols.. Samples containing 10000 cells each were carried out following 10X Genomic Chromium Single Cell 3ʹ v3 pipeline Samples were carried out following 10X Genomic Chromium Single Cell 3ʹ v3 pipeline. No feature barcoding is used.
characteristics.characteristic: description
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: mixed pool of 3 individuals
characteristics.characteristic: individual
characteristics.text: Trypanosoma evansi Merzouga 93
characteristics.characteristic: infect
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: spleen
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: C57BL6J
characteristics.characteristic: strain
characteristics.text: Pool
characteristics.characteristic: title