characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-11-14T00:46:45Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-11-14T00:46:45Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10166890
characteristics.characteristic: External Id
characteristics.text: Centre for Genome Enabled Biology and Medicine University of Aberdeen
characteristics.characteristic: INSDC center name
characteristics.text: 2023-11-14T00:46:45Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-11-14T00:46:45Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7823365
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10986:13A-3609c
characteristics.characteristic: Submitter Id
characteristics.text: 2
characteristics.characteristic: batch
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Candida albicans cells were harvested by centrifugation (5,000 rpm, room temp) and the cell pellets flash frozen in liquid nitrogen. Candida albicans cells were incubated overnight in 5 ml volumes in minimal GYNB medium, and then transferred to fresh GYNB medium in 50 mL culture flasks for 5 hours: (a) 1% lactate; (b) 0% lactate control; (c) hypoxia; or (d) normoxic control (30C, 200 rpm). RNA was prepared from frozen cell pellets via Qiazol/chloroform extraction (QIAgen) according to the manufacturer’s instructions and assessed for quality by nanodrop. RNA was DNAse treated (TURBO DNAse, Ambion) according to the manufacturers instructions and assessed for quality by Agilent Bioanalyser (RIN>8). ERCC spike in controls were added to RNA samples to be used as internal controls. Libraries were prepared with the Illumina TruSeq Stranded mRNA kit following manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: none
characteristics.characteristic: environmental stress
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: GYNB medium
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Candida albicans
characteristics.characteristic: organism
characteristics.text: Candida albicans
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: normal beta glucan masking
characteristics.characteristic: phenotype
characteristics.text: Candida albicans
characteristics.characteristic: scientific_name
characteristics.text: 3609
characteristics.characteristic: strain
characteristics.text: 13A-3609c
characteristics.characteristic: title