characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-11-03T00:39:07Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-11-03T00:39:07Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11305863
characteristics.characteristic: External Id
characteristics.text: INSERM
characteristics.characteristic: INSDC center name
characteristics.text: 2023-11-03T00:39:07Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-11-03T00:39:07Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8952650
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11197:A306_dilue
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Sciatic nerves from P4 animals of different genotypes were dissected in PBS Total RNA from was extracted using RNeasy Mini kit (Qiagen) according to the manufacturer’s instructions. After DNAse treatment, concentration and quality of RNA was determined using the NanoDrop (Thermofisher) and Xpose (Trinean) apparatus. RNAseq libraries were prepared starting from 60 ng of total RNA using the Universal Plus mRNA-Seq (Nugen) as recommended by the manufacturer. The oriented cDNA produced from the poly-A+ fraction were PCR amplified (15 cycles).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Adar1 conditional knockout
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: sciatic nerve
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: A306_dilue
characteristics.characteristic: title