characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-10-11T17:00:52Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-11T17:00:52Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113400622
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF MANCHESTER
characteristics.characteristic: INSDC center name
characteristics.text: 2023-10-11T17:00:52Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-11T17:00:52Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15395763
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12909:EJ4_Quiescent_rep4
characteristics.characteristic: Submitter Id
characteristics.text: 4
characteristics.unit: month
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: hepatic stellate cell
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mouse livers were perfused under terminal anaesthesia with pronase/collagenase solution before excision, mechanical disruption and filtering steps to generate a cell suspension. Hepatic stellate cells were isolated using an optiprep density gradient. For quiescent samples, hepatic stellate cells were freshly processed without culture. For activated samples, hepatic stellate cells were cultured on plastic in 16% FBS supplemented media for 10 days prior to harvest into RLT buffer. Activated samples were from hepatic stellate cells cultured on plastic for 10 days in 16% FBS supplemented media. Cells were harvested in RLT buffer and processed using a Qiagen RNeasy kit following manufacturer’s instructions. Library preparation of samples was performed using the Illumina TruSeq Stranded mRNA Sample Preparation Kits (Illumina).
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: mouse 4
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: quiescent
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: EJ4_Quiescent_rep4
characteristics.characteristic: title