characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-11-22T00:28:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-11-22T00:28:01Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112299827
characteristics.characteristic: External Id
characteristics.text: Institute for Plant Science in Montpellier - CNRS
characteristics.characteristic: INSDC center name
characteristics.text: 2023-11-22T00:28:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-11-22T00:28:01Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14410650
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12483:3
characteristics.characteristic: Submitter Id
characteristics.text: 5
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Col-0
characteristics.characteristic: cultivar
characteristics.text: Protocols: Samples were collected in liquid nitrogen and directly stored at -80 Plants were grown in hydroponics in ambient or elevated CO2 (900 PPM), low (0,5 mM) of high (10 mM) nitrate provision, and iron starvation or not. RNA were extracted using column-based protocols from RNA biology company After the QC procedures, mRNA were enriched using oligo(dT) beads. The mRNA were fragmented randomly by adding fragmentation buffer, then the cDNA were synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina), dNTPs, RNase H and DNA polymerase I were added to initiate the second-strand synthesis. After a series of terminal repair, ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.
characteristics.characteristic: description
characteristics.text: rosette growth stage
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: root
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: 3
characteristics.characteristic: title