characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-10-31T00:26:45Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-31T00:26:45Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112648136
characteristics.characteristic: External Id
characteristics.text: Dept. of Clinical and Molecular Medicine Norwegian University of Science and Technology (NTNU)
characteristics.characteristic: INSDC center name
characteristics.text: 2023-10-31T00:26:45Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-31T00:26:45Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14645859
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12626:KO6_1w
characteristics.characteristic: Submitter Id
characteristics.text: 73
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: GGTAGC,ATCACG
characteristics.characteristic: barcode
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: For stool collection, previously cohabitating mice littermates were separated into individual cages for a duration of 30 minutes. After said period all stool pellets were collected, snap frozen in liquid nitrogen and stored at -80C until further processing. Tamoxifen (Sigma-Aldrich, T5648-1G) was dissolved in 50ml of Corn Oil (Sigma-Aldrich, C8267-500ML) at a 20 mg/ml concentration and stored at 4°C. To induce Lsd1 recombination, 8-12-week-old mice were administered daily via oral gavage with 0.1ml of Tamoxifen for a duration of 5 days. Genomic DNA extraction from frozen stool (snap frozen in liquid N2) samples was done using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Cat. No. 51604) according to protocol. Lysis temperature was adjusted to 95℃. 16S rDNA Amplicon Metagenomic Sequencing of bacterial gDNA derived from mouse stool was outsourced to Novogene UK. Amplicon library of the V4 region was obtained using the 515F (5’-GTGCCAGCMGCCGCGGTAA-3') and 806R (5’- GGACTACHVGGGTWTCTAAT -3') primer pairs connected with barcodes (see sample table for barcodes). The PCR products with proper size were selected by 2% agarose gel electrophoresis. Same amount of PCR products from each sample was pooled, end-repaired, A-tailed and further ligated with Illumina adapters.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Villin-CreERT2 +; Lsd1 fl/fl
characteristics.characteristic: genotype
characteristics.text: KO6
characteristics.characteristic: individual
characteristics.text: GTGCCAGCMGCCGCGGTAA,GGACTACHVGGGTWTCTAAT
characteristics.characteristic: linker primer sequence
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: feces
characteristics.characteristic: organism part
characteristics.text: Villin driven knockout of Lsd1
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: KO6_1w
characteristics.characteristic: title