characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-11-09T00:26:45Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-11-09T00:26:45Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112196177
characteristics.characteristic: External Id
characteristics.text: UCL Institute of Healthy Ageing
characteristics.characteristic: INSDC center name
characteristics.text: 2023-11-09T00:26:45Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-11-09T00:26:45Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14307250
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12440:Sample 13
characteristics.characteristic: Submitter Id
characteristics.text: 10
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: fruit fly
characteristics.characteristic: common name
characteristics.text: Protocols: Fly dissection on ice for less than 1.5 hr. Collect the brains in PBS. Centrifuge the brains at 2000 rpm at 4 degree C for 5 minutes. Substitute the supernatant PBS with 50 uL dispase (3mg/ml) and 75 ul collagenase (100mg/ml). Incubate the brains with the enzymes at 25 degree C in a 500 rpm shaker for 5 minutes. Pipetting the enzymes solution containing the brains for another 10 minutes to fully dissociate brains. Centrifuge the dissociated cells at 4 degree C 2000 rpm for 5 minutes and wash the cells with 200 ul 0.04% BSA in PBS. Pre-wet the 10 um cell strainer. Pass the cell suspension through the strainer and collect the filtered cell suspension, and the sample is ready for viability check. 10X chromium 3' 10X chromium 3'
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: UAS36R; elavGS
characteristics.characteristic: genotype
characteristics.text: 13
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: Drosophila melanogaster
characteristics.characteristic: organism
characteristics.text: brain
characteristics.characteristic: organism part
characteristics.text: Drosophila melanogaster
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: w1118
characteristics.characteristic: strain
characteristics.text: Sample 13
characteristics.characteristic: title