characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-01T05:28:42Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-01T05:28:42Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113403310
characteristics.characteristic: External Id
characteristics.text: Genomix4Life
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-01T05:28:42Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-01T05:28:42Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15398443
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12911:13WT-G13
characteristics.characteristic: Submitter Id
characteristics.text: 3
characteristics.unit: month
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: A miRNA analysis on Mitochondria of 4 tissues (liver, gastrocnemius skeletal muscle, testis, white adipose tissue) was performed by RNA-seq in 3 wild-type male CD1 mice, ~3 months old, maintained in a temperature-controlled room at 22 ± 2°C under a 12 h dark/light cycle with a standard pellet diet and unlimited access to water. Mitochondria were purified from freshly excised tissues (liver, gastrocnemius skeletal muscle, testis, white adipose tissue) using Qproteome Mitochondrial Isolation kit (Qiagen) according to manufacturer’s instructions with minor modifications and performing all the steps at 4°C. In order to remove RNA of nuclear origin present in the cytosol and eventually adsorbed on the outer mitochondrial membrane, mitochondria were treated with RNase A solution. Total RNA was purified from mitochondria by miRNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol. The RNA integrity and concentration was evaluated by Agilent 2100 Bioanalyzer (Agilent Technologies). The mitochondrial RNA enrichment and eventual cytosolic RNA contamination were checked by RT-qPCR. Mitochondria and cytosolic RNAs were retrotranscribed by SensiFAST cDNA Synthesis kit (Bioline). Indexed libraries were prepared with NEXSmallRNA Seq v3 (PerkinElmer) according to the manufacturer’s instructions. Libraries were quantified using the Tape Station 2200 (Agilent Technologies) and Qubit Fluorometer 1.0 (Invitrogen Co., Carlsbad, CA). Libraries were pooled such that each index-tagged sample was present in equimolar amounts, with final concentration of the pooled samples of 2nM.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: 6
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: gastrocnemius
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: CD1 mus strain
characteristics.characteristic: strain
characteristics.text: 13WT-G13
characteristics.characteristic: title