characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-14T00:21:00Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-14T00:21:00Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113596028
characteristics.characteristic: External Id
characteristics.text: Istituto di Ricerche Farmacologiche \"Mario Negri\" IRCCS
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-14T00:21:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-14T00:21:00Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15590121
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13055:36_MNHOC266_OLA_24h
characteristics.characteristic: Submitter Id
characteristics.text: 6
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Tumors were transplanted in female Athymic Nude-Foxn1nu mice 6-weeks old obtained from Envigo Laboratories. For the xenograft generation, patient-derived ovarian cancer ascites fluid was collect and transplanted intraperitoneally (i.p.) in the mice. When mice developed ascites, the ascetic fluid was collected, tumor cells pelleted and transplanted again i.p. in other mice. Stabilized PDXs were then used for in vivo activities. For RNAseq experiments, ovarian cancer ascites were collect from the mice, cells pelleted and snap-frozen. Before library preparation, RNA concentration was evaluated through Qubit™ RNA High Sensitivity Assay Kit (Invitrogen, Walthman, MA, USA) while RNA quality was established using 4200 Tapestation (Agilent Technologies, Santa Clara, CA, USA). Protocol: miRNeasy Mini Kit. Following the TruSeq Stranded Total RNA protocol (Illumina, San Diego, CA, USA), at least 500 ng of RNA whose RIN value was between 7 and 9, were used for RNA sequencing. TruSeq Stranded Total RNA Sample Preparation Guide. Following the TruSeq Stranded Total RNA protocol (Illumina, San Diego, CA, USA), at least 500 ng of RNA whose RIN value was between 7 and 9, were used for RNA sequencing.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: ovarian serous adenocarcinoma
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: human-derived xenograft grown in mice
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: ovarian cancer ascites
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: Foxn1nu
characteristics.characteristic: strain
characteristics.text: 36_MNHOC266_OLA_24h
characteristics.characteristic: title
characteristics.text: patient-derived xenograft from ovarian carcinoma ascites
characteristics.characteristic: xenograft