characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-11-23T12:00:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-11-23T12:00:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112193812
characteristics.characteristic: External Id
characteristics.text: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
characteristics.characteristic: INSDC center name
characteristics.text: 2023-11-23T12:00:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-11-23T12:00:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14304889
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12439:NC_empty_rep1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: AF22
characteristics.characteristic: cell_line
characteristics.text: fibroblast
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were collected using Trypsin‐EDTA (0.025%, Thermo Fisher Scientific). AF22 Neuroepithelial Stem cells (NES) were cultured as adherent cells on cell culture flasks previously coated with 20 μg/ml polyornithine (Sigma) for 1 hour and 1 μg/ml Laminin2020 for 4 hours (Sigma). Cells were cultured on NES culture medium contained DMEM/F12+GlutaMax (Gibco), supplemented with 10 μl/ml N‐2‐supplement (100×, Thermo Fisher Scientific), 10 μl/ ml Penicillin‐Streptomycin (10,000 U/ml, Thermo Fisher Scientific), 1 μl/ml B27‐supplement (50×, Thermo Fisher Scientific), 10 ng/ml of bFGF (Life Technologies) and 10 ng/ ml of FGF (PeproTech). The culture medium was replaced every second day. The NES cells were passaged enzymatically when reaching 100% confluency using Trypsin‐EDTA (0.025%, Thermo Fisher Scientific). NES cells were seeded at a density of 40,000 cells/cm2. Nes cells were seeded at the density of 18750 cell/cm2 and let inside the cell culture incubator to attach for 6 hours. Cells were stimulated with different Jag1Fc nanopatterns for 3 hours. RNA extraction was performed by using RNeasy Micro Kit (Qiagen). Libraries were prepared with TruSeq stranded mRNA (Illumina 20020595) according to manufactures instructions.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: dermis
characteristics.characteristic: organism part
characteristics.text: 1
characteristics.characteristic: replicate
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: NC_empty_rep1
characteristics.characteristic: title