characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-11-01T00:24:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-11-01T00:24:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112145468
characteristics.characteristic: External Id
characteristics.text: Moores Cancer Center, University of California San Diego, La Jolla 92093, United States
characteristics.characteristic: INSDC center name
characteristics.text: 2023-11-01T00:24:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-11-01T00:24:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14255226
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12377:Sample 6 #634
characteristics.characteristic: Submitter Id
characteristics.text: 79
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: dendritic cell
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: BMDCs collected from a 79-day-old female mouse were cultured with 5 uM compound #634, or 0.1% DMSO. 5 h later the cells were lysed in RNA lysis Buffer for RNA extraction. Total RNA was isolated using the Quick-RNA Miniprep Kit (Zymo Research, R1055) according to the manufacturer’s instructions. Biological triplicates were obtained for each genotype. 1 ug of each sample’s total RNA was then prepared into barcoded mRNA libraries, according to manufacturer’s instructions, using the Illumina® Stranded mRNA Prep kit (Illumina, #20040534).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: mouse 1
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: bone marrow
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: Sample 6 #634
characteristics.characteristic: title