characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-01T05:28:15Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-01T05:28:15Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110691484
characteristics.characteristic: External Id
characteristics.text: Ghent University, Faculty of Veterinary Medicine, Deparment of Translational Physiology, Infectiology, and Public Health (DI04), Research group of Virology
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-01T05:28:15Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-01T05:28:15Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12789043
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12120:Pig3_Lung_CD163Sn_LCM
characteristics.characteristic: Submitter Id
characteristics.text: 8
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: conventional Belgian
characteristics.characteristic: breed
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: macrophage
characteristics.characteristic: cell_type
characteristics.text: pig
characteristics.characteristic: common name
characteristics.text: Protocols: To further investigate these macrophage subsets, total RNA sequencing was performed in parallel on two subsets from the nasal mucosa and lung macrophages. Macrophages were isolated by either fluorescent activated cell sorting (FACS; cf. bulk RNAseq) or laser capture microdissection (LCM; cf. LCM RNAseq) in combination with immunofluorescence staining against two macrophage markers, CD163 and Sialoadhesin (Sn). RNA was extracted using RNeasy Micro Kit (Qiageon; 74004) for bulk RNAseq subset, or using Single Shot Lysis Buffer for LCM RNAseq subset. Libraries were constructed using TruSeq Stranded mRNA Kit for bulk RNAseq subset, or using SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara, 634413) for LCM RNAseq subset.
characteristics.characteristic: description
characteristics.text: piglet
characteristics.characteristic: developmental stage
characteristics.text: CD163+; Sn+
characteristics.characteristic: immunophenotype
characteristics.text: pig3
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Sus scrofa
characteristics.characteristic: organism
characteristics.text: Sus scrofa
characteristics.characteristic: organism
characteristics.text: lung
characteristics.characteristic: organism part
characteristics.text: Sus scrofa
characteristics.characteristic: scientific_name
characteristics.text: Pig3_Lung_CD163Sn_LCM
characteristics.characteristic: title