characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-10-20T13:00:41Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-20T13:00:41Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112155527
characteristics.characteristic: External Id
characteristics.text: Southern University of Science And Technology
characteristics.characteristic: INSDC center name
characteristics.text: 2023-10-20T13:00:41Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-20T13:00:41Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14265424
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12389:hESC_Con1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: WAe001-A
characteristics.characteristic: cell_line
characteristics.text: embryonic stem cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Total RNA was purified by RNeasy Minikit (Qiagen) according to the manufacturer’s manual. RNA concentration was determined by Nanodrop, and 500 ng of total RNA was used for library construction using KAPA Stranded mRNA-Seq Kits. Sequencing was performed on Illumina HiSeq 2500 machines with 125 bp pair-end mode. Mouse E14 ESCs were cultured in DMEM (Hyclone) supplemented with 10% FCS (Gibco), 1xNEAA (Gibco), 1 mM sodium pyruvate (Gibco), 0.1 mM 2-mercaptoethanol (Sigma), 2 mM L-glutamine (Gibco), and LIF (Millipore) on 0.1% gelatin (Sigma) coated plates. Total RNA was extracted using the RNeasy Minikit (Qiagen) based on the manufacturer's instructions. Library was prepared using KAPA Stranded mRNA-Seq Kits based on the manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: embryoid body
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: inner cell mass
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.characteristic: strain
characteristics.text: hESC_Con1
characteristics.characteristic: title