characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-11-21T00:50:44Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-11-21T00:50:44Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112239346
characteristics.characteristic: External Id
characteristics.text: Junior Research Group Somatic Evolution and Early Detection German Cancer Research Center (DKFZ), Heidelberg, Germany
characteristics.characteristic: INSDC center name
characteristics.text: 2023-11-21T00:50:44Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-11-21T00:50:44Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14350236
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12455:xg_001-tumor-bo-r-none-04-x
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: SCC25-GFP-Luc
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: One million SCC25-GFP-Luc cells mixed with; i) one million mouse (MEFs), ii) one million naked mole-rat fibroblasts or, iii) no fibroblasts. 100 μl of cell mixtures were injected subcutaneously to both flanks of 8-week old female immunosuppressed NSG mice. Tumors were collected 28 days after injection SCC25 tumors at day 28 were homogenized in lysis buffer from the Qiagen AllPrep mini kit using Tissue Lyzer II (Qiagen). Total RNA was then isolated using Qiagen AllPrep mini kit (Qiagen) and DNase digested (AM1907, Invitrogen) according to manufactures instructions. RNA concentration was measured using a NanoDrop spectrophotometer(Thermo Fisher): RNA integrity was assessed using RNA ScreenTapes(Agilent Technologies). The RNA integrity number (RIN) was above 8.6 forall samples. 100 ng DNase treated RNA was used to generate ribo-minustreated total RNA stranded libraries using Illumina Stranded Total RNAPrep Ligation with Ribo-Zero Plus kit (Illumina) according to manufacturesinstructions.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: squamous cell carcinoma
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: monoculture
characteristics.characteristic: growth condition
characteristics.text: xg_001
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: tongue
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: xg_001-tumor-bo-r-none-04-x
characteristics.characteristic: title
characteristics.text: human squamous carcinoma cells xenografted into nsg mice until neoplasm formation
characteristics.characteristic: xenograft