characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-10-10T00:21:41Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-10T00:21:41Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111498676
characteristics.characteristic: External Id
characteristics.text: Karolinska Institutet, Scilifelab
characteristics.characteristic: INSDC center name
characteristics.text: 2023-10-10T00:21:41Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-10T00:21:41Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13592440
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12325:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: MDAMB231 & THP1
characteristics.characteristic: cell_line
characteristics.text: cancer
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Spheroid were labelled for 2 hours with Calcein-AM and Vybrant-DiD both at 10uM at 37C. The spheroids were collected, washed twice in PBS, digested for 5 minutes with TrypsinLE 0.25% and quenched Spheroid culture media. The cell suspension was washed twice with PBS and live single cells were sorted according to the smartseq3xpress protocol. smartseq3xpress protocol smartseq3xpress protocol
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: breast cancer & monocytic leukemia
characteristics.characteristic: disease
characteristics.text: DMEM 10%FBS 37C
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: breast & blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 1
characteristics.characteristic: title