characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-15T00:21:18Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-15T00:21:18Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10107975
characteristics.characteristic: External Id
characteristics.text: Commissariat a l'Energie Atomique
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-15T00:21:18Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-15T00:21:18Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7764536
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10931:Sample 11
characteristics.characteristic: Submitter Id
characteristics.text: 27
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: 9
characteristics.unit: year
characteristics.characteristic: age at chernobyl accident
characteristics.text: 27
characteristics.unit: year
characteristics.characteristic: age at diagnosis
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: not applicable
characteristics.characteristic: delay between accident and birth
characteristics.text: Protocols: The Chernobyl Tissue Bank (CTB) provided the post-Chernobyl series of thyroid tumors and non tumoral tissues adjacent to the tumors, as Genomic DNA samples. All informations used for samples annotation were provided by the CTB. The confirmation of diagnosis was performed by the International Pathology Panel of the CTB, which is supported by NCI grant number U24CA082102: Dr A Abrosimov, Pr T Bogdanova, Pr G Fadda, Professor J Hunt, Pr M Ito, Pr V LiVolsi, Pr J Rosai, Pr ED Williams. Tumors and adjacent non tumoral tissues were considered as radiation-induced and exposed, respectively, when the patients were under 15 years old (period of high thyroid radiation sensitivity), and lived in oblasts of Ukraine highly contaminated at the time of the Chernobyl accident. The series also included Ukrainian patients, born in the same oblasts than radiation-induced cases after March 1987 and therefore considered at as non-exposed (sporadic PTC) due to radioactive iodines short decay. DNA quantity and quality was assessed by spectophotometry (260/280 and 260/230 ratio) using NanoDrop and by fluorometry using Qbit DNA HS assay (ThermoFisher scientific, France). Genomic DNA quality was verified on a Bioanalyzer 2100 (Agilent Technologies, USA). Whole Exome Sequencing (WES) has been performed by the Centre National de Recherche en Génomique Humaine (CNRGH), Institut de Biologie François Jacob, Evry, FRANCE. Once quality controlled by the DNA and Cell Bank laboratory, genomic DNA (200 ng) has be captured using in-solution enrichment methodology (Human All Exon v6 – 60 Mb, Agilent Technologies, CA, USA). Library preparation and exome enrichment protocol (~20.000 targeted genes) have been performed automatically on NGSx (Perkin Elmer Inc, MA, USA) and Bravo (Agilent Technologies, CA, USA) respectively, according to manufacturer’s instructions (SureSelect, Agilent Technologies CA, USA).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: papillary thyroid carcinoma
characteristics.characteristic: disease
characteristics.text: exposed to radioiodines during childhood
characteristics.characteristic: environmental history
characteristics.text: No PTC, no RAS or BRAF mutation
characteristics.characteristic: genetic alterations
characteristics.text: UA0853
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Kiev
characteristics.characteristic: oblast
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: thyroid gland
characteristics.characteristic: organism part
characteristics.text: neoplasm
characteristics.characteristic: sampling site
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: 0.0354
characteristics.unit: Gray
characteristics.characteristic: thyroid dose
characteristics.text: Sample 11
characteristics.characteristic: title