characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-02T17:00:05Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-02T17:00:05Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113597540
characteristics.characteristic: External Id
characteristics.text: Lund University
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-02T17:00:05Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-02T17:00:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15591633
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13059:Sample 2
characteristics.characteristic: Submitter Id
characteristics.text: 5 to 8
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Col-0
characteristics.characteristic: cultivar
characteristics.text: Protocols: Flowers were collected from mature plants and immediately frozen Plants were grown in soil-filled pots in long day conditions (16h light) at approximately 120 umol s-1 m-2. Mitoribosome footprints were prepared from Arabidopsis flower buds as previously described (Planchard et al., 2018). Ribosome footprints were depleted from ribosomal RNAs after first strand cDNA synthesis using custom biotinylated oligonucleotides matching major rRNA contaminants found in previous experiments (Planchard et al., 2018). N. Planchard, P. Bertin, M. Quadrado, C. Dargel-Graffin, I. Hatin, O. Namy, H. Mireau, The translational landscape of Arabidopsis mitochondria. Nucleic acids research 46, 6218-6228 (2018). Ribo-Seq libraries were prepared using the TruSeq Small RNA library preparation kit (Illumina).
characteristics.characteristic: description
characteristics.text: whole plant flowering stage
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: flower
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: Sample 2
characteristics.characteristic: title