characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-10-31T00:26:35Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-31T00:26:35Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111510054
characteristics.characteristic: External Id
characteristics.text: Department of Chemical Engineering and Biotechnology - University of Cambridge
characteristics.characteristic: INSDC center name
characteristics.text: 2023-10-31T00:26:35Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-31T00:26:35Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13620966
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12334:SM2A
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Cultures of E. coli BL21 (DE3) - control and evolved - were harvested in stationary phase and 2 x 10^9 cells were collected for processing. DNA from E. coli BL21 (DE3) samples was extracted using the DNeasy Blood & Tissue Kit (Qiagen) according to the manufacturer’s instructions for gram-negative bacteria. The sequencing library was constructed from the isolated genomic DNA using the NEB DNA Ultra II kit - dual indexing (New England Biolabs). The library was re-sequenced using a MiSeq Reagent Kit v2 (Illumina) in a 500 cycle (2 x 250 bp pair-end reads) reaction on the MiSeq instrument (Illumina).
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: terrific broth media pH9
characteristics.characteristic: growth condition
characteristics.text: Escherichia coli BL21(DE3)
characteristics.characteristic: organism
characteristics.text: Escherichia coli BL21(DE3)
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: evolved strain
characteristics.characteristic: phenotype
characteristics.text: Escherichia coli BL21(DE3)
characteristics.characteristic: scientific_name
characteristics.text: BL21 (DE3)
characteristics.characteristic: strain
characteristics.text: SM2A
characteristics.characteristic: title