characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-10-20T00:36:55Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-20T00:36:55Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10418849
characteristics.characteristic: External Id
characteristics.text: Laboratory of Traslational Research. Azienda USL-IRCCS di Reggio Emilia
characteristics.characteristic: INSDC center name
characteristics.text: 2023-10-20T00:36:55Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-20T00:36:55Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8071693
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11049:sgRUNX2_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: TPC-1
characteristics.characteristic: cell_line
characteristics.text: epithelial cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were collected after 10 days from selection. Cells were cultured in DMEM medium supplemented with 10% FBS and 1% P/S. Cells were infected with pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro containing either sgRUNX2 or sgNT, then selected with 1ug/ml Puromycin for 3 days. Total RNA was extracted by Maxwell RSC simplyRNA Cells kit (Promega, Madison, Wisconsin, USA) Libraries were obtained starting from 100ng of total RNA following the Illumina Stranded Total RNA preparation Ligation with Ribo-Zero Plus protocol.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: papillary thyroid carcinoma
characteristics.characteristic: disease
characteristics.text: pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro_sgRUNX2
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: thyroid gland
characteristics.characteristic: organism part
characteristics.text: transcriptional repression of RUNX2
characteristics.characteristic: phenotype
characteristics.text: 2
characteristics.characteristic: replicate
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: sgRUNX2_2
characteristics.characteristic: title