characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-30T00:24:52Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-30T00:24:52Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113971288
characteristics.characteristic: External Id
characteristics.text: Department of Immunology, Genetics and Pathology, Cancer Precision Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-30T00:24:52Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-30T00:24:52Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15963191
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13136:DMSO1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: INA-6
characteristics.characteristic: cell_line
characteristics.text: plasma cell
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: The MM authenticated cell line INA-6 were selected for UNC1999 treatment. 100,000 cells/mL was seeded into flasks. 24h post seeding 1 µM of UNC1999 or DMSO (Sigma-Aldrich; cat. No 317275) was added to the cells. Media, supplements, and drug were renewed every 3rd day until end point and day 5. All experiments were conducted in 3 biological triplicates. RNA extraction was done by utilizing RNeasy Micro and/or Mini Kit (Netherlands, Qiagen; cat. no 74004/74104). 1 µg of total RNA was used for sequencing library preparation using TruSeq stranded total RNA library preparation kit with ribosomal depletion using RiboZero Gold (Illumina Inc.).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: multiple myeloma
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: bone marrow
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: DMSO1
characteristics.characteristic: title