characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-29T00:23:10Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-29T00:23:10Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113987310
characteristics.characteristic: External Id
characteristics.text: Center for Biotechnology - Bielefeld University
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-29T00:23:10Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-29T00:23:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15971192
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13150:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Protocols: To collect E. larynx, two standard nylon net samples (30 x 50 cm) were submerged at 10 m depth between net pens at the SINTEF ACE research salmon farm site in Vinjefjorden, mid Norway, on 11th of August 2020. The samples were photographed weekly to monitor growth and were retrieved 23 days later, on 3rd of September. Samples were transferred to the SINTEF SeaLab laboratory in Trondheim in seawater and stored over night at 2°C. Taxonomic identification was carried out the following day based on Schuchert (2010), using a dissecting microscope. Individual hydroid polyps were cleaned of other biofouling organisms (e.g., algae) or associated fauna (e.g., caprellid shrimp) using pure ethanol. Total RNA was extracted from two samples of E. larynx using ZymoBIOMICS RNA Miniprep Kit (Zymo Research), following kit instructions. The RNA output was then reverse transcribed to cDNA using QuantaBio qScript cDNA SuperMix (Quantabio) following manufacturer protocol. Two polyA enriched mRNA libraries were built using TruSeq stranded mRNA kit (Illumina) according to the library prep kit instructions.
characteristics.characteristic: description
characteristics.text: mixed
characteristics.characteristic: developmental stage
characteristics.text: SINTEF ACE research salmon farm site in Vinjefjorden, mid Norway
characteristics.characteristic: environmental history
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Ectopleura larynx
characteristics.characteristic: organism
characteristics.text: Ectopleura larynx
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Ectopleura larynx
characteristics.characteristic: scientific_name
characteristics.text: Sample 1
characteristics.characteristic: title