characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-11-09T16:00:06Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-11-09T16:00:06Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112483387
characteristics.characteristic: External Id
characteristics.text: Max Planck Institute for Biology of Ageing
characteristics.characteristic: INSDC center name
characteristics.text: 2023-11-09T16:00:06Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-11-09T16:00:06Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14600738
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12560:Young
characteristics.characteristic: Submitter Id
characteristics.text: 3
characteristics.unit: month
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: hepatocyte
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Liver nuclei (n=4) were prepared from frozen tissue specimens by crushing and dounce homogenising the tissue in 1 ml EZbuffer (SIGMA) (20 strokes with loose and a tight pestle, respectively) and spun 5 min at 300 g. The pellet was incubated on ice for 20 min in EZ-buffer supplemented with DNAseI NEB M0303S (4 units/ml) and 1X DNAseI buffer. Equal volume of EZ-buffer was added and samples were spun 5 min at 500 g and incubated again 10 min on ice in EZ-buffer supplemented with DNAseI NEB M0303S (8 units/ml) and 1X DNAseI buffer. Equal volume of EZ-buffer was added, and samples were spun 5min at 500g, resuspended in NSB (1087.5 µl 1XPBS, 5.5µl 2% BSA, 1.5 µl RNase Inhibitor) and filtered 3 times through a 0.22 µm strainer. For scATAC-seq, 100,000 nuclei were resuspended in 50 µl tagmentation mix. scATAC-seq targeting 4000 cells per sample was performed using a beta version of Chromium Single Cell ATAC Library and Gel Bead kit (10x Genomics, 1000110) according to the manufacturer’s instructions.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: mixed pool of 4 mice
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6N
characteristics.characteristic: strain
characteristics.text: Young
characteristics.characteristic: title