characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-22T00:22:12Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-22T00:22:12Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112330130
characteristics.characteristic: External Id
characteristics.text: Department of Microbiology and Immunology, University of Otago
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-22T00:22:12Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-22T00:22:12Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14441040
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12526:Efaecalis_WT_TXB1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: 30 ml cultures were mixed with equivolume 3:2 glycerol saline and harvested by centrifugation at 10,000 x g for 15 minutes at -10 degrees celcius. Cells were resuspended in 1ml 1:1 glycerol saline and stored at -80 degrees celcius. Enterococcus faecalis wild-type and the croRS deletion mutant were grown in biological triplicate in BHI broth to an OD600 of 0.5 at 37 degrees celcius and 130 rpm. Following growth to an OD600 of 0.5, cultures were split in two, with half treated with teixobactin (0.5 ug/ml) and the other half untreated and grown for a further 1 h. TRIzol/chloroform RNA extraction, followed by RNA clean and concentrate using a Zymo RNA cleand and concentrate kit. RNA libraries were prepped using the Zymo-Seq Ribofree Total RNA-Seq Library kit.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: BHI broth
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Enterococcus faecalis
characteristics.characteristic: organism
characteristics.text: Enterococcus faecalis
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Enterococcus faecalis
characteristics.characteristic: scientific_name
characteristics.text: Efaecalis_WT_TXB1
characteristics.characteristic: title