characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-08-31T01:38:00Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-08-31T01:38:00Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113940691
characteristics.characteristic: External Id
characteristics.text: Interfacutly Bioinformatics Unit, Unversity of Bern
characteristics.characteristic: INSDC center name
characteristics.text: 2023-08-31T01:38:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-08-31T01:38:00Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15934943
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13128:library
characteristics.characteristic: Submitter Id
characteristics.text: 30 to 44
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: Large white
characteristics.characteristic: breed
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: T3 porcine bronchiolar epithelial cell line
characteristics.characteristic: cell_line
characteristics.text: bronchiolar epithelial cell
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: pig
characteristics.characteristic: common name
characteristics.text: Protocols: At 24 or 48 hours p.i., the cells were washed twice and cells were lysed using TRIzol Reagent (Life Technologies). The T3 porcine bronchiolar epithelial cell line was generated by InSCREENeX GmbH (Braunschweig, Germany) and kindly provided by Sasha Trapp (Institut national de la recherche agronomique, INRA, France). The cells were maintained at 37°C and 5% CO2 in porAEC medium with supplement (InSCREENeX, INS-ME-1024) in collagen-coated (InSCREENeX, INS-SU-1017) flasks Cells grown in 24-well cell culture plates were inoculated with virus using an MOI of 1 TCID50/cell. Poly I:C was used at a concentration 1/10000. Following an incubation for 90 minutes at 37°C, the inoculum was removed, the cells washed three times with PBS, and fresh porAEC medium added, then cells were returned to the CO2 incubator at 37°C. RNA was extracted using NucleoSpin RNA (Macherey Nagel cat# 740955.50). Library preparation was performed using BRB-Seq library preparation kit (Alithea genomics, PN 10813) following manufacturer’s instruction.
characteristics.characteristic: description
characteristics.text: juvenile stage
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: mixed
characteristics.characteristic: individual
characteristics.text: mixed
characteristics.characteristic: infect
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Sus scrofa
characteristics.characteristic: organism
characteristics.text: Sus scrofa
characteristics.characteristic: organism
characteristics.text: lung
characteristics.characteristic: organism part
characteristics.text: Sus scrofa
characteristics.characteristic: scientific_name
characteristics.text: library
characteristics.characteristic: title