characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-01T05:28:43Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-01T05:28:43Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113416752
characteristics.characteristic: External Id
characteristics.text: UCL GOS Institute of Child Health, University College London
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-01T05:28:43Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-01T05:28:43Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15411883
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12925:P6
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: primary cell line
characteristics.characteristic: cell_line
characteristics.text: keratinocyte
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Primary keratinocytes were isolated from patients with inflammatory skin disease in addition to controls which were subsequently immortalised (Lenti-HPV-16 E6/E7) RNA was extracted using the RNAeasy plus mini kit (Qiagen, Germany), using RLT buffer to lyse cells, 70-90% ethanol to precipitate the nucleic acids and nuclease free water to resuspend the RNA. cDNA libraries were prepared using the KAPA mRNA HyperPrep Kit (Roche)
characteristics.characteristic: description
characteristics.text: juvenile
characteristics.characteristic: developmental stage
characteristics.text: inflammatory skin disease
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: skin
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: P6
characteristics.characteristic: title