characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-11-23T00:24:38Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-11-23T00:24:38Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11294745
characteristics.characteristic: External Id
characteristics.text: Advanced Centre for Treatment Research and Education in Cancer (ACTREC)
characteristics.characteristic: INSDC center name
characteristics.text: 2023-11-23T00:24:38Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-11-23T00:24:38Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: MMP10 shRNA
characteristics.characteristic: RNA interference
characteristics.text: ERS8944307
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11185:AD2940_CAL27_MMP10_sh67
characteristics.characteristic: Submitter Id
characteristics.text: 56
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: CAL27
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Each sample was collected by scrapping the cells from a 10cm cell culture dish. Pellet was stored at -20 degrees. AW13516, AW8507 and CAL27 cells were cultured in DMEM medium. The media was supplemented with 10% FBS, 2.5 mg/ml Amphotericin-B (Abbott) and 1.25 μl/ml Gentamycin (Abbott). The cells were cultured at 37 °C in a 5% CO2 incubator. RNA was extracted using the TRIzol solubilization and extraction protocol, as recommended by the manufacturer. Transcriptome sequencing libraries were constructed by using the TruSeq RNA library protocol (Illumina). Briefly, mRNA was purified from 4 μg of intact total RNA using oligodT beads and library preparation was done as per the manufacturer's instructions (TruSeq RNA Sample Preparation Kit, Illumina).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: tongue squamous cell carcinoma
characteristics.characteristic: disease
characteristics.text: European
characteristics.characteristic: ethnic group
characteristics.text: none
characteristics.characteristic: genetic modification
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: tongue
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: AD2940_CAL27_MMP10_sh67
characteristics.characteristic: title