characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-01T05:28:43Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-01T05:28:43Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113424990
characteristics.characteristic: External Id
characteristics.text: Center for Omics Sciences IRCCS San Raffaele Scientific Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-01T05:28:43Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-01T05:28:43Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15420118
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12948:HT29_FOLFIRI_16H_1
characteristics.characteristic: Submitter Id
characteristics.text: 44
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HT29
characteristics.characteristic: cell_line
characteristics.text: colorectal cancer cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were trypsinized, harvested and washed with PBS 1%. Then, the pellet was immediately used for RNA extraction or frozen and stocked at -80°C until use. HCT116 cells were grown in DMEM supplemented with 10% Fetal Bovine Serum. HT29 cells were grown in RPMI supplemented with 10% Fetal Bovine Serum and 1mM Sodium Pyruvate. Cells were plated, and after 24h were treated with FOLFOX or FOLFIRI at the following concentration: 5-FU 100µM + OXA 100µM (FOLFOX) or 5-FU 100µM + SN-38 100nM (FOLFIRI) for 8h or 16h. Then, cells were harvested for experimental purposes. For long term (2 weeks) treatments, cells were plated and exposed to 5-FU 10µM + OXA 1µM (FOLFOX) or 5-FU 10µM + SN-38 10nM (FOLFIRI). RNA extraction was performed using the RNeasy kit (74106) from Qiagen. Ribo minus total RNA stranded kit was used.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: colorectal adenocarcinoma
characteristics.characteristic: disease
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: intestine
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: HT29_FOLFIRI_16H_1
characteristics.characteristic: title