characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-08-31T18:00:42Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-08-31T18:00:42Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112868170
characteristics.characteristic: External Id
characteristics.text: Institut d'Investigacio Illes Balears (IdISBa)
characteristics.characteristic: INSDC center name
characteristics.text: 2023-08-31T18:00:42Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-08-31T18:00:42Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14874819
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12821:231-IE8dis-2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: MDAMB231
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were collected using 0.25% Trypsin-EDTA 1x (Gibco) Cells were cultured using RPMI 1640 media complemented with 10% FBS and penicillin/streptomycin at 5% CO2 37ºC. Cells were transiently transfected with either empty pSpCas9 or pSpCas9 containing a sgRNA sequence against insulator element 8. Transfection was performed using Lipofectamine3000 following manufacturer’s guidelines. Cells were then sorted using FACSAria Fusion using GFP+ as a reporter. The established models were then subcultured to perform high throughput experiments. Cell nuclei from, 50.000 cells were processed following OmniATAC protocol (Nat. Meth. Corces 2017). ATAC-seq library was performed according to Buenrostro JD, Giresi PG, Zaba LC, Chang HY, Greenleaf WJ. 2013.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: triple-negative breast cancer
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: mammary gland
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 231-IE8dis-2
characteristics.characteristic: title