characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-10-01T00:27:22Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-01T00:27:22Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112154515
characteristics.characteristic: External Id
characteristics.text: Service de Medecine Interne, Centre national de reference des cytopenies auto-immunes de l’adulte, Hopital Henri Mondor, Federation Hospitalo-Universitaire TRUE InnovaTive theRapy for immUne disordErs, Assistance Publique Hopitaux de Paris (AP-HP), Universite Paris Est Creteil, Creteil, France.
characteristics.characteristic: INSDC center name
characteristics.text: 2023-10-01T00:27:22Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-01T00:27:22Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14264378
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12397:DE-015-0003-DE
characteristics.characteristic: Submitter Id
characteristics.text: 78
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: nongranular leukocyte
characteristics.characteristic: cell_type
characteristics.text: sustained complete response off-treatment
characteristics.characteristic: clinical history
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: ScRNA-seq analyses were performed on frozen PBMCs isolated from heparin blood. Samples originated from 8 patients at baseline (4 with SCROT and 4 with SROT). PBMCs were thawed according to 10X Genomics protocol. Cells were counted, diluted at 1,000 cells/mL in PBS+0.04% and 20,000 cells were loaded in the 10x Chromium Controller to generate single-cell gel-beads in emulsion. After reverse transcription, gel-beads in emulsion were disrupted. Barcoded complementary DNA was isolated and amplified by PCR. Following fragmentation, end repair and A-tailing, sample indexes were added during index PCR.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: autoimmune thrombocytopenia
characteristics.characteristic: disease
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: DE-015-0003-DE
characteristics.characteristic: title