characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-10-01T00:27:18Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-01T00:27:18Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111476174
characteristics.characteristic: External Id
characteristics.text: Co-author
characteristics.characteristic: INSDC center name
characteristics.text: 2023-10-01T00:27:18Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-01T00:27:18Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13570210
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12298:Sample 10
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: 3T3-L1
characteristics.characteristic: cell_line
characteristics.text: adipocyte
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: 3T3-L1 fibroblasts were washed 3x in PBS and lysed in 350 µL RLT buffer. Lysed samples were collected and stored at -80°C until further analysis 3T3-L1 adipocytes were seeded and differentiated in 12-well plates. RNA extractions were performed using the RNeasy Mini Kit (Qiagen 1152 #74104) 1 μg of total RNA was quality checked (RIN >7) using an Agilent Bioanalyser 2100 system and then used to construct barcoded sequencing libraries with Illumina’s TruSeq Stranded mRNA Library Prep Kit following manufacturer’s instructions
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: low media volume
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: embryo
characteristics.characteristic: organism part
characteristics.text: fibroblast
characteristics.characteristic: progenitor cell type
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Sample 10
characteristics.characteristic: title