characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-10-12T00:21:06Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-12T00:21:06Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112769152
characteristics.characteristic: External Id
characteristics.text: SAARLAND UNIVERSITY
characteristics.characteristic: INSDC center name
characteristics.text: 2023-10-12T00:21:06Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-12T00:21:06Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14769958
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12747:Sample 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: SH-SY5Y
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Adherent cells were harvested in Trizol reagent NA Trizol protocol according to manufactorer's guidelines. At least 800 ng RNA was sent for Quality control and RNA Sequencing Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers,followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library (for further information check Novogene RNA seq protocols).
characteristics.characteristic: description
characteristics.text: juvenile stage
characteristics.characteristic: developmental stage
characteristics.text: neuroblastoma
characteristics.characteristic: disease
characteristics.text: CRISPR-Cas9 transfected with no gRNA
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: bone
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 2
characteristics.characteristic: title