characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-21T00:17:54Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-21T00:17:54Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111436286
characteristics.characteristic: External Id
characteristics.text: Institute for Research on Cancer and Aging, Nice (IRCAN) Inserm U1081 - CNRS UMR 7284
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-21T00:17:54Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-21T00:17:54Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13529961
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12245:DMSO_rep3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HCT116
characteristics.characteristic: cell_line
characteristics.text: colorectal cancer cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: HCT-116 cells were cultured in McCoy medium supplemented with 10% FBS, 100 U/mL penicillin and 100 µg/mL streptomycin. For 5-aza-deoxycytidine (DAC) treatment, cells were plated at a density of 100,000 cells/well in 6-well plates and treated with DAC at a final concentration of 1 μM for a total of 5 days. Fresh medium and drug were added daily for the first 3 days. Total RNA was purified by two successive cycles of TRI Reagent extraction Directional poly(A)+ RNA-Seq libraries were prepared using NEBNext Ultra II Directional RNA Library Prep kit for Illumina
characteristics.characteristic: description
characteristics.text: colorectal adenocarcinoma
characteristics.characteristic: disease
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: colon
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: DMSO_rep3
characteristics.characteristic: title