characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-01T05:28:48Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-01T05:28:48Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113907012
characteristics.characteristic: External Id
characteristics.text: Mario Negri Institute for pharmacological research (IRCCS)
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-01T05:28:48Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-01T05:28:48Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15901179
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13101:GFP_1_REP2
characteristics.characteristic: Submitter Id
characteristics.text: 8 to 12
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: smooth muscle cell
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mice were housed in 12 hour light/dark cycles (7am-7pm) and given ad libitum access to a standard rodent chow diet and water. 8-12-week-old male mice were used in all animal experiments. Mice were kept in the Koc University Animal Research Facility in accordance with institutional policies perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in bioRxiv preprint doi: https://doi.org/10.1101/2023.01.23.525138; this version posted January 23, 2023. The copyright holder for this 9 and animal care ethics guidelines. EDA2R-KO and NIK-floxed mice were generated by Genentech8,29 . NIK-floxed mice were a gift from Prof. Shao-Cong Sun (MD Anderson Cancer Center). Myo-NIK-KO mice were generated by crossing NIK-floxed and ACTA1-Cre mice (Jackson strain #006149). OSMR-floxed mice (strain #011081) were purchased from Jackson laboratory. Myo-OSMR-KO mice were generated by crossing OSMR flox and ACTA1-Cre mice (Jackson strain #006149). All mice were maintained on a pure C57BL/6 background. Plasma CRP levels were measured using an ELISA assay (BT Lab E0218Mo). All animal protocols were approved by the Institutional Animal Care and Use Committee of Koc University. Before library preparation, RNA concentration was evaluated through Qubit™ RNA High Sensitivity Assay Kit (Invitrogen, Walthman, MA, USA) while RNA quality was established using 4200 Tapestation (Agilent Technologies, Santa Clara, CA, USA). Protocol: miRNeasy Mini Kit. Following the TruSeq Stranded Total RNA protocol (Illumina, San Diego, CA, USA), at least 500 ng of RNA whose RIN value was between 7 and 9, were used for RNA sequencing. TruSeq Stranded Total RNA Sample Preparation Guide. Following the TruSeq Stranded Total RNA protocol (Illumina, San Diego, CA, USA), at least 500 ng of RNA whose RIN value was between 7 and 9, were used for RNA sequencing.
characteristics.characteristic: description
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: GFP (Ctrl)
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: smooth muscle
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: GFP_1_REP2
characteristics.characteristic: title