characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-11-17T00:45:23Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-11-17T00:45:23Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA11006945
characteristics.characteristic: External Id
characteristics.text: IRCCS Casa Sollievo della Sofferenza Hospital
characteristics.characteristic: INSDC center name
characteristics.text: 2023-11-17T00:45:23Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-11-17T00:45:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS8656724
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11151:Sample 79
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Each participant provided cervical fluid sample in a collection tube filled with a DNA stabilization buffer. DNA microbial extraction was performed using the QIAamp DNA Stool Mini Kit (Qiagen) starting from 1 mL of each sample according to the manufacturer’s protocol. After assessing DNA concentration and purity, samples were stored at -80° until processing. The V3–V4 hypervariable region of the bacterial 16S rRNA gene was amplified from total DNA according to the Illumina 16S Metagenomic Sequencing Library Preparation instructions, as previously described in Diamanti-Picchianti A, et al (Int J Mol Med ). In summary, the V3-V4 amplicon was obtained by PCR with universal primers, followed by purification. A second PCR was then performed to barcode the libraries using the Illumina dual-index system. Following a second step of purification, libraries were diluted to 4nM and pooled.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: infertility
characteristics.characteristic: disease
characteristics.text: N
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: human vaginal metagenome
characteristics.characteristic: organism
characteristics.text: human vaginal metagenome
characteristics.characteristic: organism
characteristics.text: cervical fluid
characteristics.characteristic: organism substance
characteristics.text: human vaginal metagenome
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: Sample 79
characteristics.characteristic: title