characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-10-11T17:00:34Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-11T17:00:34Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113400760
characteristics.characteristic: External Id
characteristics.text: UNIVERSITY OF MANCHESTER
characteristics.characteristic: INSDC center name
characteristics.text: 2023-10-11T17:00:34Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-11T17:00:34Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15395901
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12908:EJ7_Activated_rep4
characteristics.characteristic: Submitter Id
characteristics.text: 4
characteristics.unit: month
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: hepatic stellate cell
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mouse livers were perfused under terminal anaesthesia with pronase/collagenase solution before excision, mechanical disruption and filtering steps to generate a cell suspension. Hepatic stellate cells were isolated using an optiprep density gradient. For quiescent samples, hepatic stellate cells were freshly processed without culture. For activated samples, hepatic stellate cells were cultured on plastic in 16% FBS supplemented media for 10 days prior to harvest into PBS. Activated samples were from hepatic stellate cells cultured on plastic for 10 days in 16% FBS supplemented media. 100000 cells were pelleted and PBS washed. Isolation of Tn5 transposase-accessible DNA was performed using ActiveMotif ATAC-seq kit (Catalog no 53150) following manufacturer’s instructions. Library preparation of samples was performed using the ActiveMotif ATAC-seq kit (Catalog no 53150) following manufacturer’s instructions.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: mouse 4
characteristics.characteristic: individual
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: activated
characteristics.characteristic: phenotype
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: EJ7_Activated_rep4
characteristics.characteristic: title