characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-10-18T00:22:57Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-18T00:22:57Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111432003
characteristics.characteristic: External Id
characteristics.text: Surgery Department at University of Texas Southwestern Medical center at Dallas
characteristics.characteristic: INSDC center name
characteristics.text: 2023-10-18T00:22:57Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-18T00:22:57Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13525678
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12241:PANC1-Fin56-10microM-6h2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Panc1
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: 1)plate Panc-1 cells 6x105/well in 6-wells cell culture plates with DMEM medium containing 10% FBS and 1%Pen/Strep on day1. 2) cells were treated with or without RSL3,N6F11,Fin56, and Erastin for 6hours on day2. 3)Aspirate and discard the cell culture medium, wash the cells with 1XPBS buffer X2 times. 4)collect cell lysates with 35oul GTC Lysis Buffer/well. Vortex or pipet up and down to mix thoroughly. GTC lysis buffer(E.Z.N.A.® HP Total RNA Kit,Cat#R6812-02, Omega) RNA extraction: Using E.Z.N.A.® HP Total RNA Kit,Cat#R6812-02, Omega 1) Insert a RNA Homogenizer Mini Column into a 2 mL Collection Tube. 2)Transfer the lysate to the RNA Homogenizer Mini Column. 3)Centrifuge at 13,000 x g for 1 minute 4)Add 1 volume 70% ethanol. Vortex to mix thoroughly. Do not centrifugeve the filtrate and discard the RNA Homogenizer Mini Column. 5)Insert a HiBind® RNA Mini Column into a 2 mL Collection Tube. 6)Transfer 700 μL sample (including any precipitate that may have formed) to the HiBind® RNA Mini Column. 7)Centrifuge at 10,000 x g for 1 minute 8)Discard the filtrate and reuse the Collection Tube 9)Add 500 μL RNA Wash Buffer II. 10)Centrifuge at 10,000 x g for 1 minute 11)Discard the filtrate and reuse the Collection Tube 12)Centrifuge at maximum speed for 2 minutes to completely dry the HiBind® RNA Mini Column. 13)Transfer the HiBind® RNA Mini Column to a clean 1.5 mL microcentrifuge tube (not provided) 14)Add 40-70 μL Nuclease-free Water. 15)Centrifuge at maximum speed for 2 minutes then measure the RNA concentration and store eluted RNA at -80°C. An Illumina TruSeq Stranded mRNA Library prep kit (20020594) was used to generate the RNA libraries.Illumina TruSeq Stranded mRNA Sample Preparation Protocol. In briefly protocol is described as below: A: on day1  Step 1: Isolate mRNA  Step 2: 1st strand cDNA synthesis  Step 3: 2nd strand cDNA synthesis  Step 4: Reaction clean-up B: on day2  Step 5: Adenylate cDNA ends  Step 6: ligate adapters to ends  Step 7: PCR amplify library  Normalize and Pool  Setup Run in Basespace  Loading the Sequencer
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: epithelioid carcinoma
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: pancreatic duct
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: PANC1-Fin56-10microM-6h2
characteristics.characteristic: title