characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-16T00:20:54Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-16T00:20:54Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA10111020
characteristics.characteristic: External Id
characteristics.text: Laboratory of Traslational Research. Azienda USL-IRCCS di Reggio Emilia
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-16T00:20:54Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-16T00:20:54Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS7767578
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-10942:nt2_1
characteristics.characteristic: Submitter Id
characteristics.text: 62
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: MSTO-211H
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were collected 7 days after sgRNAs infection Cells were cultured in RPMI medium supplemented with 10% FBS and 1% P/S. Cells were subsequently infected with the LentiCas9-Blast and pLKO5.sgRNA.EFS.GFP containing the specific sgRNAs (TRIM28_sgRNA_49, TRIM28_sgRNA_50, NT2 (non-targeting sgRNA)) Total RNA was extracted by Maxwell RSC simplyRNA Cells kit(Promega, Madison, Wisconsin, USA) Libraries were obtained starting from 500ng of total RNA following the Illumina TruSeq Stranded Total RNA preparation protocol.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: malignant pleural mesothelioma
characteristics.characteristic: disease
characteristics.text: European
characteristics.characteristic: ethnic group
characteristics.text: CRISPR Cas9 with non-targeting sgRNA
characteristics.characteristic: genetic modification
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: pleura
characteristics.characteristic: organism part
characteristics.text: 1
characteristics.characteristic: replicate
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: nt2_1
characteristics.characteristic: title