characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2022-11-17T16:00:07Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-23T00:21:01Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111429007
characteristics.characteristic: External Id
characteristics.text: Division of Gastroenterology, IRCSS Casa Sollievo della Sofferenza, Opera di San Pio da Pietrelcina
characteristics.characteristic: INSDC center name
characteristics.text: 2022-11-17T16:00:07Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-23T00:21:01Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13522682
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12236:SP371
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Homo sapiens hospitalised patient
characteristics.characteristic: clinical history
characteristics.text: Protocols: Each sample were collected with a rectal swabs according to manufacturer's instructions. Samples were treated with 500 μl of Lysis Buffer ATL (QIAGEN, Hilden, Germany) at 56°C for 10 min with 20 μl Proteinase K (Darmstadt, Germany) before DNA extraction. Microbial DNA was extracted from 500 μl of sample using Nimbus automatic extractor (Seegene Technologies, Korea) according to the manufacturer’s protocol. The V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified from total DNA according to the Illumina 16S Metagenomic Sequencing Library Preparation instructions, as previously described in Diamanti-Picchianti A, et al (Int J Mol Med ). In summary, the V3-V4 amplicon was obtained by PCR with universal primers, followed by purification. A second PCR was then performed to barcode the libraries using the Illumina dual-index system. Following a second step of purification, libraries were diluted to 4nM and pooled.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: COVID-19
characteristics.characteristic: disease
characteristics.text: severe
characteristics.characteristic: disease staging
characteristics.text: SARS-CoV-2
characteristics.characteristic: infect
characteristics.text: gut metagenome
characteristics.characteristic: organism
characteristics.text: gut metagenome
characteristics.characteristic: organism
characteristics.text: feces
characteristics.characteristic: organism part
characteristics.text: rectal swab
characteristics.characteristic: sampling site
characteristics.text: gut metagenome
characteristics.characteristic: scientific_name
characteristics.text: SP371
characteristics.characteristic: title