characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-23T00:21:01Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-23T00:21:01Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111431603
characteristics.characteristic: External Id
characteristics.text: SYSU
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-23T00:21:01Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-23T00:21:01Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13525278
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12239:B1_2-1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Transcriptomic analysis was conducted to measure the transcription of these sulfur metabolism genes in DvH cultures amended with two different molar ratios of lactate to sulfate, i.e., 1:1 and 2:1. Briefly, samples for total RNA extraction were collected by centrifugation (5 min, 10,000×g, 4°C) from triplicate cultures at mid-exponential phase (OD600 = ~0.4), and then mixed with TRIzolTM reagent (Invitrogen; Waltham, MA, USA). Total RNA was extracted with a RNeasy Mini kit (Qiagen), from which fragmented DNA was removed using DNase I. The highly transcribed ribosomal RNAs (rRNAs) were eliminated using the RiBoCop rRNA depletion Kit (Lexogen; Vienna, Austria). The RNA-Seq libraries were constructed from the rRNA-depleted RNA samples using NEB Next® Ultra™ Directional RNA Library Prep Kit (New England Biolabs; Ipswich, MA, USA)
characteristics.characteristic: description
characteristics.text: logarithmic phase culture
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Desulfovibrio vulgaris str. Hildenborough
characteristics.characteristic: organism
characteristics.text: Desulfovibrio vulgaris str. Hildenborough
characteristics.characteristic: organism
characteristics.text: Desulfovibrio vulgaris str. Hildenborough
characteristics.characteristic: scientific_name
characteristics.text: lactate:sulfate 2:1
characteristics.characteristic: stimulus
characteristics.text: B1_2-1
characteristics.characteristic: title