characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-01T05:28:19Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-01T05:28:19Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111348488
characteristics.characteristic: External Id
characteristics.text: VANDERBILT UNIVERSITY
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-01T05:28:19Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-01T05:28:19Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13446035
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12170:8047-MC-0016
characteristics.characteristic: Submitter Id
characteristics.text: 12
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: The interscapular brown adipose tissue (iBAT) was harvested from mice following euthanasia using carbon dioxide at 5 liters per minute for 3 minutes. Prkd1 WT or brown adipose tissue KO (background: C/57Bl6) mice were maintained on chow diet and acclimated at thermoneutrality for 2 days (30 degrees Celsius) followed by either 8 hours or 4 days at 6 degrees Celsius in the Powers temperature regulated cabinet. iBAT was harvested and placed in 350 uL Trizol solution. The sample was prepared according to the Trizol User Reagent Guide through step 8. Following Trizol extraction, samples were placed on Qiagen RNA extraction columns according to the manufacturer's protocol until purified RNA was obtained. RNA was amplified using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara), and cDNA libraries were constructed using the Low Input Library Prep Kit (Takara).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: cold temperature regimen
characteristics.characteristic: environmental stress
characteristics.text: Prkd1 -/-
characteristics.characteristic: genotype
characteristics.text: 16
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: interscapular fat pad
characteristics.characteristic: organism part
characteristics.text: interscapular brown adipose tissue
characteristics.characteristic: sampling site
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: 8047-MC-0016
characteristics.characteristic: title