characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-22T00:22:02Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-22T00:22:02Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA111436896
characteristics.characteristic: External Id
characteristics.text: Institute for Research on Cancer and Aging, Nice (IRCAN) Inserm U1081 - CNRS UMR 7284
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-22T00:22:02Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-22T00:22:02Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS13530571
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12247:K562
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: K562
characteristics.characteristic: cell_line
characteristics.text: chronic myelogenous leukemia cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were grown and collected from culture dishes. Genomic DNA was extracted using the Monarch Genomic DNA Purification kit. 125 sgRNAs targeting the region downstream of L1HS elements (one unique sgRNA per locus) were synthesized using the EnGen sgRNA Synthesis kit (New England Biolabs). Targeted librairy preparation follows these steps: assembly of Cas9 ribonucleoprotein particles (RNP), dephosphorylation of genomic DNA, incubation of RNP assemble with genomic DNA, library preparation and Oxford Nanopore sequencing. With this design, sequencing starts at the sgRNA binding site in the region downstream of L1 and progresses towards the target L1 in the antisense direction
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: chronic myelogenous leukemia
characteristics.characteristic: disease
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: bone marrow
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: K562
characteristics.characteristic: title