characteristics.text: ERC000022
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-10-13T16:22:35Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-13T16:22:35Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110188087
characteristics.characteristic: External Id
characteristics.text: INRA-CMSE
characteristics.characteristic: INSDC center name
characteristics.text: 2023-10-13T16:22:35Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-13T16:22:35Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12286891
characteristics.characteristic: SRA accession
characteristics.text: BOU_T0_R1_18S
characteristics.characteristic: Submitter Id
characteristics.text: 2021
characteristics.characteristic: collection date
characteristics.text: 2 mm
characteristics.characteristic: composite design/sieving (if any)
characteristics.text: crop
characteristics.characteristic: current land use
characteristics.text: The three soils used in this experiment were sampled in February 2021 from the ploughed horizon (0-20 cm) of three French regions representing the pedoclimatic diversity in France. They are located in Burgundy (BOU, Rendisols (WRB, I.W.G., 2015) – continental climate, 47°38’86.88N; 5°06’29.01E), Pays de la Loire (PDL, Brunisols (WRB, I.W.G., 2015) – oceanic climate, 47°72’86.90N; 0°49’71.88W) and Provence-Alpes-Côte d’Azur (PACA, Rankosols (WRB, I.W.G., 2015) – Mediterranean climate, 43°17’80.55N; 6°20’88.88E
characteristics.characteristic: description
characteristics.text: agricultural soil
characteristics.characteristic: environment (biome)
characteristics.text: microbial feature
characteristics.characteristic: environment (feature)
characteristics.text: soil
characteristics.characteristic: environment (material)
characteristics.text: France
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 0.2
characteristics.unit: m
characteristics.characteristic: geographic location (depth)
characteristics.text: 313
characteristics.unit: m
characteristics.characteristic: geographic location (elevation)
characteristics.text: 47.3886887
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 5.0629013
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: BURGUNDY
characteristics.characteristic: geographic location (region and locality)
characteristics.text: eukaryote
characteristics.characteristic: investigation type
characteristics.text: 15
characteristics.unit: ºC
characteristics.characteristic: mean annual and seasonal temperature
characteristics.text: 48.48
characteristics.unit: g/kg
characteristics.characteristic: microbial biomass
characteristics.text: Molecular microbial biomass were determined in 1% agarose gel electrophoresis. A calf thymus DNA standard curve was used to estimate the molecular microbial biomass (Dequiedt et al., 2011) and quantified by bromide ethidium intensity with ImageQuantTL (Molecular Dynamics, Evry, France) software
characteristics.characteristic: microbial biomass method
characteristics.text: CGACGTGACT(forward) CGCGACATCT(reverse)
characteristics.characteristic: multiplex identifiers
characteristics.text: The PCR conditions were an initial step of 3 min at 94 °C, 35 cycles of 30 s at 94 °C, 1 min at 52 °C and 1 min at 72 °C, followed by 5 min at 72 °C (Chemidlin Prévost-Bouré et al., 2011)
characteristics.characteristic: nucleic acid amplification
characteristics.text: Microbial DNA was extracted from 1 g (dry weight) of soil according to a standardized procedure of the GenoSol platform (INRAE, Dijon, France, https://www2.dijon.inrae.fr/plateforme_genosol/) (Terrat et al., 2012)
characteristics.characteristic: nucleic acid extraction
characteristics.text: soil metagenome
characteristics.characteristic: organism
characteristics.text: 7.96
characteristics.characteristic: pH
characteristics.text: pH in a water solution (NF ISO 10390)
characteristics.characteristic: pH method
characteristics.text: Primers FR1 (5’-ANC CAT TCA ATC GGT ANT-3’) and FF390 (5’-CGA TAA CGA ACG AGA CCT-3’) (Chemidlin Prévost-Bouré et al., 2011)
characteristics.characteristic: pcr primers
characteristics.text: Metha-BioSol
characteristics.characteristic: project name
characteristics.text: 1
characteristics.unit: g
characteristics.characteristic: sample weight for DNA extraction
characteristics.text: soil metagenome
characteristics.characteristic: scientific_name
characteristics.text: Illumina
characteristics.characteristic: sequencing method
characteristics.text: soil
characteristics.characteristic: soil environmental package
characteristics.text: Rendisols
characteristics.characteristic: soil_taxonomic/local classification
characteristics.text: -40
characteristics.unit: °C
characteristics.characteristic: storage conditions (fresh/frozen/other)
characteristics.text: 18S
characteristics.characteristic: target gene
characteristics.text: V7-V8 region
characteristics.characteristic: target subfragment
characteristics.text: 8/53/39
characteristics.unit: % sand/silt/clay
characteristics.characteristic: texture
characteristics.text: Particle size distribution (NF X 31-107)
characteristics.characteristic: texture method
characteristics.text: BOU_T0_R1
characteristics.characteristic: title
characteristics.text: 1.75
characteristics.unit: µg/L
characteristics.characteristic: total nitrogen
characteristics.text: total carbon (NF ISO 10694) and nitrogen (N) content (NF ISO 13878)
characteristics.characteristic: total nitrogen method
characteristics.text: total carbon (NF ISO 10694) and nitrogen (N) content (NF ISO 13878)
characteristics.characteristic: total organic C method
characteristics.text: 20.21
characteristics.unit: g/kg
characteristics.characteristic: total organic carbon
characteristics.text: 0.33
characteristics.unit: cm3/cm3
characteristics.characteristic: water content
characteristics.text: The water-holding capacity (WHC) was estimated in the laboratory (gravimetric humidity)
characteristics.characteristic: water content method