characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-10-12T00:21:14Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-10-12T00:21:14Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA113546315
characteristics.characteristic: External Id
characteristics.text: Umea university, department of molecular biology
characteristics.characteristic: INSDC center name
characteristics.text: 2023-10-12T00:21:14Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-10-12T00:21:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15542736
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13001:OP_N2_4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Protocols: Twenty young adult animals were picked to the fresh plates and allowed to lay eggs for 2 hours, after which the adult animals were removed and eggs were allowed to develop into L4 stage. Ten plates of L4 animals were washed off and placed on either OP50 or V. cholerae containing plates for 8 hours. Subsequently, the animals were collected and rinsed three times with M9 buffer. After washing, the worm pellet was frozen in liquid nitrogen. Animals were homogenized using Bullet Blender (Next Advance) in the presence of Qiazol Lysis Reagent and 0.5mm Zirconia beads at 4°C. RNA was prepared using RNeasy Plus Universal Mini Kit (Qiagen). Library was constructed and sequenced by Novogene
characteristics.characteristic: description
characteristics.text: L4 larva
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: mixed pool of 20 individuals
characteristics.characteristic: individual
characteristics.text: Escherichia coli OP50
characteristics.characteristic: infect
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: organism
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: scientific_name
characteristics.text: mixed
characteristics.characteristic: sex
characteristics.text: N2
characteristics.characteristic: strain
characteristics.text: OP_N2_4
characteristics.characteristic: title