characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-09-30T00:24:36Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-09-30T00:24:36Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA112751274
characteristics.characteristic: External Id
characteristics.text: The Blizard Institute Barts and The London School of Medicine and Dentistry, Queen Mary, University of London
characteristics.characteristic: INSDC center name
characteristics.text: 2023-09-30T00:24:36Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-09-30T00:24:36Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14745964
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12736:Egr2/3_DKO_FR4+Egr2-_VV_1
characteristics.characteristic: Submitter Id
characteristics.text: 12
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: T cell
characteristics.characteristic: cell_type
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: CD4+CD25-CD44hiFR4+Egr2-/-Egr3-/- cells were isolated from Egr2/3 DKO mice by FACS GFP-Egr2 knockin (Egr2 Kin) and hCD2-Cre Egr2loxP/loxP Egr3-/- (Egr2/3 DKO) mice were infected intraperitoneally with 4 x 10^6 PFU of vaccinia virus. CD4 T cells were isolated 21 days after infection RNA was isolated and purified using the RNeasy UCP Micro Kit (Qiagen) according to the manufacturer's instructions RNA from T cells was processed for directional mRNA-Seq library construction using the KAPA mRNA HyperPrep Kit (Kapa Biosystems) in combination with IDT xGen UDI-UMI adapters (Integrated DNA Technologies) according to the manufacturers’ protocols
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: hCD2-Cre Egr2loxP/loxP Egr3-/-
characteristics.characteristic: genotype
characteristics.text: FR4+Egr2-
characteristics.characteristic: immunophenotype
characteristics.text: mixed pool from 10 mice
characteristics.characteristic: individual
characteristics.text: vaccinia virus
characteristics.characteristic: infect
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: mixed lymph node and spleen
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: mixed
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: Egr2/3_DKO_FR4+Egr2-_VV_1
characteristics.characteristic: title