ERC000011
ENA-CHECKLIST
2023-12-01T00:42:43Z
ENA-FIRST-PUBLIC
2023-12-01T00:42:43Z
ENA-LAST-UPDATE
SAMEA113904641
External Id
Laboratory for Hematology and Immunology, Department of Biomedical Sciences, Vrije Universiteit Brussel, Brussels, Belgium
INSDC center name
2023-12-01T00:42:43Z
INSDC first public
2023-12-01T00:42:43Z
INSDC last update
public
INSDC status
ERS15898808
SRA accession
E-MTAB-13095:Sample 11
Submitter Id
ArrayExpress
broker name
KG1a
cell_line
not collected
collection date
human
common name
Protocols: AML cell lines were pre-treated with siRNA against S100A9 for 24 hours and were afterwards incubated with ABT-199 for an additional 24 hours. Total RNA was extracted and purified using the NucleoSpin RNA plus kit (Macherey–Nagel, Düren, Germany). Sample quality was checked by calculating the RNA integrity number (RIN value) using fragment analyser (Agilent), with 3 biological replicates from each group. The RNA sequencing (RNA-seq) library preparation was performed with 150 ng RNA using the Illumina KAPA RNA HyperPrep kit with RiboErase (HMR) (Illumina, Cambridge, UK).
description
adult
developmental stage
acute myeloid leukemia
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
bone marrow
organism part
Homo sapiens
scientific_name
Sample 11
title