ERC000011
ENA-CHECKLIST
2023-12-01T00:42:39Z
ENA-FIRST-PUBLIC
2023-12-01T00:42:39Z
ENA-LAST-UPDATE
SAMEA113547107
External Id
College of Health, Medicine and Life Sciences, Division of Biosciences, Brunel University London
INSDC center name
2023-12-01T00:42:39Z
INSDC first public
2023-12-01T00:42:39Z
INSDC last update
public
INSDC status
ERS15543528
SRA accession
E-MTAB-12978:ACCX11_5
Submitter Id
ArrayExpress
broker name
ACCX11
cell_line
secretory cell
cell_type
human
common name
Protocols: Cells were detached by addition of trypsin. Cells suspensions were quenched with medium and centrifuged at 400 x g for 5 minutes to pellet for subsequent extraction. Total RNA was extracted using RNeasy Mini Kit (QIAGEN). Library construction was performed by Novogene UK (Cambridge, UK). A minimum of 1 ug (20 ng/μl) of high-quality total RNA (using Monarch Total RNA Miniprep Kit according to manufacturer's instructions, New England Biolabs, Hitchin, UK) was supplied for sequencing. Novogene UK constructed cDNA libraries by purification of messenger RNA using poly-A enrichment. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification.
description
adult
developmental stage
adenoid cystic carcinoma
disease
wild type genotype
genotype
Homo sapiens
organism
Homo sapiens
organism
salivary gland
organism part
Homo sapiens
scientific_name
ACCX11_5
title