ERC000011
ENA-CHECKLIST
2023-12-01T00:42:58Z
ENA-FIRST-PUBLIC
2023-12-01T00:42:58Z
ENA-LAST-UPDATE
SAMEA114538660
External Id
IGBMC
INSDC center name
2023-12-01T00:42:58Z
INSDC first public
2023-12-01T00:42:58Z
INSDC last update
public
INSDC status
ERS16519991
SRA accession
E-MTAB-13490:w1118_cns_n1
Submitter Id
5 days after hatching
day
age
ArrayExpress
broker name
not collected
collection date
fruit fly
common name
Protocols: L3 CNS (10 per replicate) were quickly dissected on ice in PBS, put directly in 150µl TRIzol™ (Invitrogen, ref 15596026) once a full sample was collected and stored on ice. CNS were lysed with 10 strokes of microtube pestle (pestle was rinsed with another 150µl TRIzol™), vortex at full speed for 1min and incubate 10 min at RT for full lysis. 1/5 V of Chloroform was added and samples were vortexed 1min at full speed, incubated 5 min at RT and centrifuged at 16000g for 30 min at 4°C. Supernatant was collected, and RNA was extracted with 1V of isopropanol and glycogen (3 µg/mL final concentration), incubate at -20°C for 2h and centrifuge at 16000g for 30 min at 4°C. Pellets were washed twice in ethanol 75%, 15 min at 16000g 4°C, allowed to dry quickly, resuspend in 30µl H2O, treated with 25U DNAse I (Thermo Scientific™ 89836) for 5 min in 50µL total volume at RT. RNA was extracted a second time in 500µL TRIzol™ like previously described. End washing steps were performed 3 times. Total RNA was resuspended in 15µL H20, 2µL were kept for downstream analysis and samples were immediately stored at -80°C till processing. RNA integrity was analyzed on Agilent RNA Chip on bioanalyzer 2100. Library preparation was performed at the GenomEast platform (IGBMC) using using the Illumina® Stranded mRNA Prep.
description
L3 larva
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
pool of 10
individual
not applicable
isolate
Drosophila melanogaster
organism
Drosophila melanogaster
organism
central nervous system
organism part
Drosophila melanogaster
scientific_name
female
sex
W1118
strain
w1118_cns_n1
title