ERC000013
ENA-CHECKLIST
2023-11-29T00:22:57Z
ENA-FIRST-PUBLIC
2023-11-29T00:22:57Z
ENA-LAST-UPDATE
SAMEA11325645
External Id
UNIVERSITY OF EXETER
INSDC center name
2023-11-29T00:22:57Z
INSDC first public
2023-11-29T00:22:57Z
INSDC last update
public
INSDC status
ERS8970876
SRA accession
18S:Ts25
Submitter Id
2017-10
collection date
Tilapia fish skin swab
description
freshwater biome (ENVO:00000873)
environment (biome)
pond (ENVO:00000033)
environment (feature)
skin environment (ENVO:2100003)
environment (material)
Malawi
geographic location (country and/or sea)
not collected
DD
geographic location (latitude)
not collected
DD
geographic location (longitude)
Maldeco, Mangochi
geographic location (region and locality)
skin mucous
host body product
fish skin
host body site
Tilapia
host common name
omnivorous
host diet
healthy
host disease status
25
cm
host length
1448876
host taxid
host-associated
host-associated environmental package
mimarks-survey
investigation type
https://dx.doi.org/10.17504/protocols.io.bw8gphtw
nucleic acid extraction
fish metagenome
organism
98 �C for 30 seconds; 30 cycles of 98 �C for 10 seconds, 60 �C for 30 seconds, 72 �C for 30 seconds; and a final extension of 72 �C for 2 minutes
pcr conditions
1391F (5'-GTACACACCGCCCGTC-3') and EukBr (5'-TGATCCTTCTGCAGGTTCACCTAC-3')
pcr primers
Tilapia skin microbiomes Malawi
project name
swab
sample collection device or method
stored in RNAlater
sample material processing
fish metagenome
scientific_name
Illumina MiSeq
sequencing method
18S rRNA
target gene
V9
target subfragment
Tilapia 3 Pond 6
title