ERC000011
ENA-CHECKLIST
2023-11-30T00:23:40Z
ENA-FIRST-PUBLIC
2023-11-30T00:23:40Z
ENA-LAST-UPDATE
SAMEA12009092
External Id
European Research Institute for the Biology of Ageing University Medical Center Groningen
INSDC center name
2023-11-30T00:23:40Z
INSDC first public
2023-11-30T00:23:40Z
INSDC last update
public
INSDC status
ERS9656095
SRA accession
E-MTAB-11247:417s
Submitter Id
9.9
month
age
ArrayExpress
broker name
tumor single-cell suspension
cell_type
house mouse
common name
Protocols: Mice were sacrificed when ill, i.e. displaying signs of hematopoietic malignancies including weight loss, lethargic behaviour, anaemia and dyspnea. Malignancies were dissected out and homogenised using a 100 um tissue strainer. Homogenised cell suspensions were centrifuged and taken up in 10% DMSO and 10% FBS in RPMI and stored at -80 degrees Celsius until RNA isolation. RNA was isolated using the RNeasy mini kit (Qiagen) and RNA quality was examined using the Agilent 2100 Bioanalyzer (Agilent Technologies). Typically 300 ng of total RNA was used as input for enrichment by NEXTflex Poly(A) Beads (Bioo Scientific) followed by library preparation using NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific).
description
adult
developmental stage
Mad2 PiggyBac Mx1-Cre
genotype
417
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
spleen
organism part
Mus musculus
scientific_name
female
sex
mixed C57BL/6 genetic background
strain
417s
title