ERC000011
ENA-CHECKLIST
2023-12-01T00:42:53Z
ENA-FIRST-PUBLIC
2023-12-01T00:42:53Z
ENA-LAST-UPDATE
SAMEA114349256
External Id
The Hebrew University of Jerusalem, Israel
INSDC center name
2023-12-01T00:42:53Z
INSDC first public
2023-12-01T00:42:53Z
INSDC last update
public
INSDC status
ERS16338300
SRA accession
E-MTAB-13341:WT_1_DMEM
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: SGH10 bacteria were grown under DMEM (Dulbecco's Modified Eagle Medium) and LB(Lysogeny broth). Bacteria were harvested by centrifugation. The bacterial pellet was resuspended in TE buffer, mixed with lysosyme (SigmaAldrich) in a final concentration of 0.9 mg/ml, and immediately frozen in liquid nitrogen. The frozen pellet was subjected to two cycles of thaw and freeze at 37°C and in liquid nitrogen, respectively, and RNA was extracted using Tri-Reagent (Sigma-Aldrich) RNAtag-seq with mudifications detailed in Melamed et al, Nature Protocols 2018
description
not collected
geographic location (country and/or sea)
DMEM media
growth condition
exponential
growth phase
not applicable
isolate
Klebsiella pneumoniae
organism
Klebsiella pneumoniae
organism
Klebsiella pneumoniae
scientific_name
SGH10
strain
WT_1_DMEM
title