ERC000011
ENA-CHECKLIST
2023-12-01T00:42:37Z
ENA-FIRST-PUBLIC
2023-12-01T00:42:37Z
ENA-LAST-UPDATE
SAMEA113446105
External Id
Botnar Research Centre Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences University of Oxford
INSDC center name
2023-12-01T00:42:37Z
INSDC first public
2023-12-01T00:42:37Z
INSDC last update
public
INSDC status
ERS15441211
SRA accession
E-MTAB-12805:PBMCs_TAB_AS1802_GEX
Submitter Id
48
year
age
ArrayExpress
broker name
mononuclear cell
cell_type
human
common name
Protocols: 1M PBMC were cultured in 200 μl medium in non-tissue culture treated 96-well round bottom plates (Corning) and left unstimulated or treated with 200 ng/ml LPS (Enzo Bioscience) or anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) for 16 h before being prepared for sequencing. After cell culture, cells were harvested and washed in PBS with 0.1% BSA. 20,000 cells from each condition were put into a channel of Chromium Next GEM Chip K of 10x Genomics platform. 10x 5' v2 10x 5' v2
description
adult
developmental stage
ankylosing spondylitis
disease
wild type genotype
genotype
AS1802
individual
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
female
sex
PBMCs_TAB_AS1802_GEX
title