ERC000011
ENA-CHECKLIST
2023-12-01T00:42:18Z
ENA-FIRST-PUBLIC
2023-12-01T00:42:18Z
ENA-LAST-UPDATE
SAMEA112328721
External Id
Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen
INSDC center name
2023-12-01T00:42:18Z
INSDC first public
2023-12-01T00:42:18Z
INSDC last update
public
INSDC status
ERS14439636
SRA accession
E-MTAB-12520:Sample 25
Submitter Id
16
week
age
ArrayExpress
broker name
house mouse
common name
Protocols: The mice were fasted overnight (11 hours) prior to liver isolation, and livers were kept at -80°C until analysis. C57Bl/6JRj mice (8 weeks of age) received either a long acting glucagon analogue (NNC9204-0043; terminal half-life of 5-6 hours, 1.5 nmol/kg twice daily) or PBS+1% BSA (twice daily). Drugs were administered over a period of 8 weeks, by subcutaneous injection. The initial dose of the glucagon analogue of 3 nmol/kg was reduced to 1.5 nmol/kg after 16 days of treatment, due to a weight decrease of >20% of one of the mice (the mouse was terminated and excluded from the study). Raw sequencing data were processed using bcl2fastq software version 2.20.0 (Illumina). Total DNA/RNA was purified by an AllPrep DNA/RNA Minikit (catalog no. 80204; QIAgen, Hilden, Germany) according to manufacturer’s instructions and quality tested using a 2100 Bioanalyzer instrument (Agilent Genomics, Santa Clara, California, USA). The extracted RNA was DNase treated with RNeasy Minikit (catalog no. 74106; QIAgen, Hilden, Germany) and RNase-Free DNase set (catalog no. 79254; QIAgen, Hilden, Germany) according to manufacturer’s protocol. All the above-mentioned processes were performed on a QIACube machine (QIAgen, Hilden, Germany). Library preparation was done using TruSeq Stranded Total RNA Library Prep Gold (catalog no. 20020599; Illumina, San Diego, California, USA) and IDT for Illumina-TruSeq RNA UD Indexes (cat 20022371; Illumina, San Diego, California, USA) according to the manufacturer’s instructions
description
normal
disease
wild type genotype
genotype
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
kidney
organism part
Mus musculus
scientific_name
female
sex
C57BL/6JRj
strain
Sample 25
title