ERC000011
ENA-CHECKLIST
2023-12-01T00:42:43Z
ENA-FIRST-PUBLIC
2023-12-01T00:42:43Z
ENA-LAST-UPDATE
SAMEA113907567
External Id
Friedrich Schiller University Jena Institute of Biodiversity Aquatic Geomicrobiology Group
INSDC center name
2023-12-01T00:42:43Z
INSDC first public
2023-12-01T00:42:43Z
INSDC last update
public
INSDC status
ERS15901734
SRA accession
E-MTAB-13102:Er.1
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Biomass was harvested from mid-exponential cultures by means of centrifugation. Pseudomonas alloputida KT2440 was grown using MP medium to mid-exponential phase, using phenylethanol (5 mM) as carbon and energy source. Cultures were grown with different lanthanide elements (La, Nd, Er, a lanthanide cocktail [Ce, Nd, Dy, Ho, Er, Yb]; 50 nM) or without lanthanide addition. Total RNA was extracted with TRI reagent (Applied Biosystems), and mRNA was enriched using the RiboMinus bacteria 2.0 transcriptome isolation kit (Invitrogen). Sequencing libraries were prepared with the NEBNext Ultra II Directional RNA library prep kit for Illumina (New England Biolabs).
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
MP medium supplemented with 5 mM pheylethanol
growth condition
not applicable
isolate
Pseudomonas putida
organism
Pseudomonas putida
organism
whole organism
organism part
1
replicate
Pseudomonas putida
scientific_name
KT2440
strain
Er.1
title