ERC000011
ENA-CHECKLIST
2023-12-09T00:33:45Z
ENA-FIRST-PUBLIC
2023-12-09T00:33:45Z
ENA-LAST-UPDATE
SAMEA114638834
External Id
Department of Cell physiology and metabolism
INSDC center name
2023-12-09T00:33:45Z
INSDC first public
2023-12-09T00:33:45Z
INSDC last update
public
INSDC status
ERS17026985
SRA accession
E-MTAB-13548:unsorted 1
Submitter Id
ArrayExpress
broker name
control
cell_line
mouse embryonic fibroblast cell
cell_type
not collected
collection date
house mouse
common name
Protocols: CAMPARI2 PC for screening was performed in 60 mm plates by lighting with 365, 385 420 nm Led light (Cholis High-Power Led source (Thorlabs) for 3 minutes at 25mW/cm2. We then FACS sorted the low 10% photoconverted cells, Tunicamycin 100ng/ml was used for 4 hours before PC isolated their genomic DNA using JetQuick™ Blood and Cell Culture DNA Midiprep Kit (A30703, Thermo). See original de brie library please Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
description
embryo
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
embryo
organism part
Mus musculus
scientific_name
unsorted 1
title