ERC000011
ENA-CHECKLIST
2023-11-19T14:00:06Z
ENA-FIRST-PUBLIC
2023-11-26T00:27:11Z
ENA-LAST-UPDATE
SAMEA114480676
External Id
Sun Yat-sun University Zhongshan Ophathlamic Center
INSDC center name
2023-11-19T14:00:06Z
INSDC first public
2023-11-26T00:27:11Z
INSDC last update
public
INSDC status
ERS16462594
SRA accession
E-MTAB-13437:Sample 18
Submitter Id
12
year
age
ArrayExpress
broker name
not collected
collection date
crab-eating macaque
common name
Protocols: macaque monkeys (6–18 years old) were used. Seven to nine sections were combined from one individual for nucleus isolation. The isolation protocol mainly comes from the published 10x Genomics protocol for nucleus isolation. In brief, the tissue was lysed via a chilled lysis kit (Sigma, no. NUC101-1KIT ). The suspension was then filtered with cell strainer and washed impurities by centrifugation with different concentrations of iodixanol. Nuclei-pellets were resuspended with a “resuspension buffer” which contained 1× phosphate-buffered saline (PBS), 1% bovine serum albumin (BSA) and 0.2 U/μl RNase inhibitor. Nuclei-pellets were suspended in the DAPI solution and incubated for inspection under the microscope. NA NA MPTP (3mg; Sigma-Aldrich, St. Louis, MO, USA) was dissolved into a concentration of 0.15mg/ml and injected singly into the left carotid artery. NA 10X single cell isolation protocol 10X Genomics Chromium platform
description
adult
developmental stage
Parkinson’s disease
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Macaca fascicularis
organism
Macaca fascicularis
organism
brain
organism part
Macaca fascicularis
scientific_name
female
sex
1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine injection
stimulus
Sample 18
title