ERC000011
ENA-CHECKLIST
2023-12-01T00:42:57Z
ENA-FIRST-PUBLIC
2023-12-01T00:42:57Z
ENA-LAST-UPDATE
SAMEA114476162
External Id
Department of Infectious Diseases, Imperial College London
INSDC center name
2023-12-01T00:42:57Z
INSDC first public
2023-12-01T00:42:57Z
INSDC last update
public
INSDC status
ERS16458122
SRA accession
E-MTAB-13403:Hfq_N-plus_3
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Samples were prepared according to the protocol found in this paper: doi:10.1038/nprot.2017.115 Wild-type and Hfq::3XFLAG E.coli were grown in Gutnick minimal media with 3mM NH4Cl as the sole nitrogen source, and cell samples were taken during exponential growth (N+), ~20min following N-runout and induction of growth arrest (N-), 24hr following N-runout and induction of growth arrest (N-24) and 2hr following addition of NH4Cl to N-24 cells (N-24+2). Samples were prepared according to the protocol found in this paper: doi:10.1038/nprot.2017.115. Final RNA purification by TRIzol extraction Preparation of strand specific cDNA performed by Vertis Biotechnologies. cDNA synthesis was conducted with oligonucleotide (dT)-adapter primers and M-MLV reverse transcriptase, followed by PCR amplification using TruSeq-designed primers from Illumina guidelines.
description
Nitrogen stress
environmental stress
Hfq-3xFLAG
genotype
not collected
geographic location (country and/or sea)
exponential growth in Gutnick minimal media with 3mM NH4Cl (N+)
growth condition
not applicable
isolate
Escherichia coli K-12
organism
Escherichia coli K-12
organism
Escherichia coli K-12
scientific_name
MG1655
strain
Hfq_N-plus_3
title