ERC000011
ENA-CHECKLIST
2023-11-28T00:25:27Z
ENA-FIRST-PUBLIC
2023-11-28T00:25:27Z
ENA-LAST-UPDATE
SAMEA112322303
External Id
Cancer Stem Cells Unit, ISBReMIT, IRCSS Casa Sollievo della Sofferenza, Opera di San Pio da Pietrelcina
INSDC center name
2023-11-28T00:25:27Z
INSDC first public
2023-11-28T00:25:27Z
INSDC last update
public
INSDC status
ERS14433220
SRA accession
E-MTAB-12486:M21T0
Submitter Id
ArrayExpress
broker name
Homo sapiens hospitalised patient
clinical history
Protocols: Fecal samples were collected at baseline, after 12 weeks and 6 months of lifestyle intervention in tubes filled with DNA stabilization buffer (CANVAX Biotech). For 16S rRNA sequencing, the total microbial DNA was extracted using the QIAamp PowerFecal DNA Kit (Qiagen) following the manufacturer’s protocol. The Illumina 16S Metagenomic Sequencing Library Preparation for high-throughput sequencing was performed as follows: 12.5 ng of each DNA extract was employed for the amplification of the V3-V4 hypervariable regions of the bacterial 16S ribosomal RNA (rRNA) gene, using the universal primers previously published by Klindworth et al. with Illumina adapters.
description
breast cancer
disease
gut metagenome
organism
gut metagenome
organism
feces
organism part
gut metagenome
scientific_name
M21T0
title