ERC000011
ENA-CHECKLIST
2023-12-10T05:00:06Z
ENA-FIRST-PUBLIC
2023-12-10T05:00:06Z
ENA-LAST-UPDATE
SAMEA112954220
External Id
Department of Microbiology, Faculty of Science, Chulalongkorn University
INSDC center name
2023-12-10T05:00:06Z
INSDC first public
2023-12-10T05:00:06Z
INSDC last update
public
INSDC status
ERS14949791
SRA accession
E-MTAB-12876:G180
Submitter Id
ArrayExpress
broker name
Protocols: S. coelicolor A3(2) was grown on MR5 plates for 24 h, then the substrate mycelia were harvested from agar by gentle scraping. Total RNA of S. coelicolor A3(2) was isolated from cells (approximately 100 mg wet weight) with Sepasol-RNA I super G reagent (Nacalai Tesque, Inc.). After treatment with RNase-free recombinant DNase I (TaKaRa Bio, Inc., Shiga, Japan), each sample was further purified using a High Pure RNA Isolation Kit (Roche Life Science, Penzberg, Germany). High-purity total RNA samples were sent to Bioengineering Lab. Co., Ltd. (Kanagawa, Japan) for the rRNA depletion using riboPOOLS (siTOOLs Biotech, Germany), and library construction using the RNA Directional Library Prep Set (MGI Tech, China).
description
SCP1+ SCP2+
genotype
modified R5 agar plate
growth condition
not applicable
isolate
Streptomyces coelicolor A3(2)
organism
Streptomyces coelicolor A3(2)
organism
haustorium
organism part
Streptomyces coelicolor A3(2)
scientific_name
1147
strain
G180
title