ERC000020
ENA-CHECKLIST
2024-02-26T16:08:26Z
ENA-FIRST-PUBLIC
2024-02-26T16:08:26Z
ENA-LAST-UPDATE
SAMEA114790180
External Id
TUBS-IPB
INSDC center name
2024-02-26T16:08:26Z
INSDC first public
2024-02-26T16:08:26Z
INSDC last update
public
INSDC status
ERS17211826
SRA accession
V14_15_tKO_5
Submitter Id
controlled environment (climate chamber)
broad-scale environmental context
2020
collection date
Set A nsp134 replicate 5
description
soil (artificial substrate mixture of potting compost, sand, vermiculite, fertilizer)
environmental medium
Germany
geographic location (country and/or sea)
52
DD
geographic location (latitude)
10
DD
geographic location (longitude)
6
weeks
host age
Arabidopsis thaliana
host common name
nsp134
host genotype
3702
host taxid
mesocosm pot experiment planted with Arabidopsis thaliana
local environmental context
ACAGTG,CAACTA
multiplex identifiers
Fast DNA SpinKit for Soil, MP Biomedicals, Germany
nucleic acid extraction
rhizosphere metagenome
organism
CCTAYGGGRBGCASCAG,GGACTACNNGGGTATCTAAT
pcr primers
rhizosphere
plant product
Amplicon sequencing of 16S rRNA gene fragments of Arabidopsis Rhizosphere
project name
vigorously shaking root cluster, removing roots by vortexing, centrifuging to obtain rhizosphere pellet
sample collection method
rhizosphere metagenome
scientific_name
Illumina NovaSeq 6000
sequencing method
16S rRNA
target gene
V3-V4 on 16S rRNA
target subfragment
SetA_tKO_5
title