ERC000011
ENA-CHECKLIST
2023-11-30T00:25:13Z
ENA-FIRST-PUBLIC
2023-11-30T00:25:13Z
ENA-LAST-UPDATE
SAMEA114624306
External Id
Bielefeld University, Omics CF NGS Unit Universitätsstraße 27,  33615, Bielefeld, Germany Bielefeld University, Medical Faculty OWL Universitätsstraße 27,  33615, Bielefeld, Germany
INSDC center name
2023-11-30T00:25:13Z
INSDC first public
2023-11-30T00:25:13Z
INSDC last update
public
INSDC status
ERS16999776
SRA accession
E-MTAB-13533:Sp_2h2_S5
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Bacteria were harvested in ice-cold killing buffer (50 mM Tris pH 7.5, 5 mM MgCl2, 20 mM NaN3), centrifuged at 4750 rpm for 10 min at 4°C and the pellets were immediately frozen in liquid nitrogen and stored at −80°C. S.p. were cultivated in supplemented RPMI medium in the presence or absence of 10% FCS, which was added to the +FCS culture at an OD600 of 0.3. The control1 culture was harvested prior to FCS treatment. The +FCS and –FCS cultures were harvested after 2 hours of growth after reaching OD600 of 0.3. The bacteria were not treteated at an OD600 of 0.3 with FCS RNA isolation was performed using an acidic phenol-chloroform extraction protocol (Wetzstein et al., 1992). After DNase-I treatment (Zymo Research, Germany), the RNA quality was checked by Trinean Xpose (Gentbrugge, Belgium) and the Agilent RNA Nano 6000 kit using an Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). For RNA-seq transcriptomics, Ribo-Zero rRNA Removal Kit (Bacteria) from Illumina (San Diego, CA, USA) was used to remove the rRNA. TruSeq Stranded mRNA Library Prep Kit from Illumina (San Diego, CA, United States) was applied to prepare the cDNA libraries.
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Streptococcus pneumoniae
organism
Streptococcus pneumoniae
organism
whole organism
organism part
Streptococcus pneumoniae
scientific_name
D39
strain
Sp_2h2_S5
title