ERC000011
ENA-CHECKLIST
2023-12-01T00:42:39Z
ENA-FIRST-PUBLIC
2023-12-01T00:42:39Z
ENA-LAST-UPDATE
SAMEA113547309
External Id
College of Health, Medicine and Life Sciences, Division of Biosciences, Brunel University London
INSDC center name
2023-12-01T00:42:39Z
INSDC first public
2023-12-01T00:42:39Z
INSDC last update
public
INSDC status
ERS15543730
SRA accession
E-MTAB-12976:ACCX11 MYB 2
Submitter Id
ArrayExpress
broker name
ACCX11
cell_line
secretory cell
cell_type
human
common name
Protocols: 5x10^6 cultured cells were used for immunoprecipitation. To crosslink proteins to DNA, 540 µl of 37% formaldehyde was added to cells in 20 ml culturing medium and incubated for 10 minutes at room temperature. 2 ml of 10X glycine was added and incubated for 5 min at room temperature. Cells were pelleted at 500 x g at 4 degrees for 5 minutes and washes in ice-cold PBS + proteinase inhibitors. Chromatin extraction, digestion, and digestion was performed using SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling) according to manufacturer's instructions. Chromatin was digested using micrococcal nuclease (MNase) prior to chromatin immunoprecipitation (ChIP) with anti-MYB antibody (recombinant anti-MYB phosphoS11, Abcam). Following ChIP, chromatin was eluted from from Antibody/Protein G Magnetic Beads and reverse crosslinked. DNA was purified by the use of spin columns. Library construction was performed by Novogene UK (Cambridge, UK). Purified DNA (extracted and digested using SimpleChIP Enzymatic Chromatin IP Kit, Cell Signaling) was supplied for sequencing. Novogene UK constructed DNA libraries as follows: DNA fragments were repaired, A-tailed, and further ligated using Illumina adapter. The final DNA library was obtained by size selection and PCR amplification.
description
adult
developmental stage
adenoid cystic carcinoma
disease
wild type genotype
genotype
Homo sapiens
organism
Homo sapiens
organism
salivary gland
organism part
Homo sapiens
scientific_name
ACCX11 MYB 2
title