ERC000011
ENA-CHECKLIST
2023-12-01T00:42:53Z
ENA-FIRST-PUBLIC
2023-12-01T00:42:53Z
ENA-LAST-UPDATE
SAMEA114350469
External Id
The Hebrew University of Jerusalem, Israel
INSDC center name
2023-12-01T00:42:53Z
INSDC first public
2023-12-01T00:42:53Z
INSDC last update
public
INSDC status
ERS16339513
SRA accession
E-MTAB-13342:WT_2_LB
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Bacteria were grown in LB (Luria Bertani) medium and in DMEM (Dulbecco's Modified Eagle Medium) to exponential phase and cells were harvested at OD600 = 0.5. Cells were peleted by centrifugation, resuspended in PBS and subjected to UV crosslinking. Then bacteria were re-collected by centrifugation and flash frozen in liquid nitrogen Following mechanical lysis of the cells, Hfq with its bound RNAs was coimmunoprecipitated using anti-Flag antibody. Exposed regions of the RNAs were trimmed by RNases, the 50 and 30 ends of the RNAs were treated with T4 PNK, neighboring RNAs were ligated, the Hfq protein was digested, and RNA was then isolated using TRI-reagent RNAtag-seq with mudifications detailed in Melamed et al, Nature Protocols 2018
description
not collected
geographic location (country and/or sea)
LB media
growth condition
exponential
growth phase
not applicable
isolate
Klebsiella pneumoniae
organism
Klebsiella pneumoniae
organism
Klebsiella pneumoniae
scientific_name
SGH10
strain
WT_2_LB
title