characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2024-01-01T01:01:09Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2024-01-01T01:01:09Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110195609
characteristics.characteristic: External Id
characteristics.text: Tata Memorial Centre - Advanced Centre for Treatment, Research and Education in Cancer
characteristics.characteristic: INSDC center name
characteristics.text: 2024-01-01T01:01:09Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-01-01T01:01:09Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12293284
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11893:PLAG1_KD_M3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: K562
characteristics.characteristic: cell_line
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Imatinib resistant K562 cells, which were established in lab, were transduced with to lentiviral particles containing shRNA 2655.2 to knockdown PLAG1 (from Sigma), and lentiviral particles containing pLKO.1-empty vectors were generated in the lab. Puromycine based selection was carried out and polyclonal populations were established for empty vector and PLAG1 knockdown. Further, with flow assisted cell sorting monoclonal populations were generated and expanded. RNA was extracted from both empty vector containing cells and PLAG1 knockdown cells. RNA was extracted from imatinib-sensitive and -resistant K562 cells, using AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, MD, USA: catalogue no. 80224) according to the manufacturer's recommendations. RNA samples were subjected to the library preparation as per NEB Ultra RNA-Seq Library Prep kit protocol (Cat# E7530L).
characteristics.characteristic: description
characteristics.text: chronic myelogenous leukemia
characteristics.characteristic: disease
characteristics.text: PLAG1 knockdown by shRNA
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: bone marrow
characteristics.characteristic: organism part
characteristics.text: imatinib resistant
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: PLAG1_KD_M3
characteristics.characteristic: title