characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2024-01-01T01:01:11Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2024-01-01T01:01:11Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110196432
characteristics.characteristic: External Id
characteristics.text: Tata Memorial Centre - Advanced Centre for Treatment, Research and Education in Cancer
characteristics.characteristic: INSDC center name
characteristics.text: 2024-01-01T01:01:11Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-01-01T01:01:11Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12294107
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11896:K562 R0.75
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: K562
characteristics.characteristic: cell_line
characteristics.text: hematopoietic cell
characteristics.characteristic: cell_type
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Gradual dose escalation protocol was used to establish imatinib-resistant K562 cell lines. K562 cells resistant to 0.25 μM, 0.5 μM, and 0.75 μM imatinib were maintained in cRPMI medium and the respective imatinib concentration and parental K562 cells were maintained in cRPMI medium only. Imatinib resistance was checked on the IC50 value for imatinib obtained from MTT assay. DNA was extracted from imatinib-sensitive and -resistant K562 cells, using AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, MD, USA: catalogue no. 80224) according to the manufacturer's recommendations. WGS was performed on imatinib-sensitive and -resistant (to 0.25, 0.5, and 0.75 M imatinib) K562 cells. Library preparation was done by 30x WGS KAPA PCR-Free v2.1 kit, as per manufacturers instruction.
characteristics.characteristic: description
characteristics.text: chronic myelogenous leukemia
characteristics.characteristic: disease
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: K562 R0.75
characteristics.characteristic: title