characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 2023-12-15T01:02:08Z
characteristics.characteristic: ENA-FIRST-PUBLIC
characteristics.text: 2023-12-15T01:02:08Z
characteristics.characteristic: ENA-LAST-UPDATE
characteristics.text: SAMEA110132328
characteristics.characteristic: External Id
characteristics.text: Unit of Animal Genomics, GIGA Institute, University of Liege
characteristics.characteristic: INSDC center name
characteristics.text: 2023-12-15T01:02:08Z
characteristics.characteristic: INSDC first public
characteristics.text: 2023-12-15T01:02:08Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS12231148
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11826:ATAC33_HOL1_m_gDNA
characteristics.characteristic: Submitter Id
characteristics.text: 1
characteristics.unit: month
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: cattle
characteristics.characteristic: common name
characteristics.text: Protocols: Blood samples were collected from jugular vein using K2-EDTA blood collection tubes. DNA was isolated from a blood sample using Quick-DNA Miniprep Plus kit (Zymo research) following manufacturer's instructions. Genomic DNA library was prepared using 50 ng of DNA (for a sample with a word, gDNA, in a file name) - 100 ng (gDNA2X) isolated from a Holstein male (HOL1_m) by following Nextera DNA Sample Preparation Guide (Illumina). The tagmented DNA was purified and eluted in 21 ul elution buffer using the MinElute PCR purification kit. The DNA was amplified using NEBNext High-Fidelity 2X PCR Master Mix with Ad1 and Ad2 primers (Buenrostro et al., 2013) for 5 PCR-cycles. The amplified libraries were purified and eluted in 51 ul elution buffer using the MinElute PCR purification kit. Library size distribution was monitored using 10 ul of the library by QIAxcel capillary electrophoresis (Qiagen). Large DNA fragments were eliminated using AMPure XP magnetic beads (Beckman Coulter, Brea, CA) by a right-side size selection using 0.55 x followed by 1.5 x ratio of beads to sample. Library concentration was estimated using KAPA Library Quantification kit (Kapa Biosystems, Wilmington, MA).
characteristics.characteristic: description
characteristics.text: juvenile stage
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: HOL1_m
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Bos taurus
characteristics.characteristic: organism
characteristics.text: Bos taurus
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Bos taurus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: Holstein
characteristics.characteristic: strain
characteristics.text: ATAC33_HOL1_m_gDNA
characteristics.characteristic: title