2024-03-05
ENA first public
ERC000011
ENA-CHECKLIST
Department of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland
INSDC center name
public
INSDC status
ERS18400096
SRA accession
E-MTAB-13859:Sample 2
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Overnight culture in Tryptic Soy Broth (TSB) with Chloramphenicol (for plasmid selection) at 37C with 220rpm shaking was diluted 1:20 in fresh medium and incubated for 1.5h. Bacteria were washed twice in RNAlater and RNA was isolated using the RNeasy Mini kit (Qiagen) following manufacturer’s protocol including DNAse treatment (Turbo DNase kit, Invitrogen).  Paired end 150 bp RNAseq was performed without rRNA depletion by the Functional Genomics Center Zurich (FGCZ) of University of Zurich and ETH Zurich using the TruSeq RNA Library Prep Kit on an Illumina NovaSeq X Plus yielding on average 47.6 million reads per sample.
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Staphylococcus aureus
organism
Staphylococcus aureus
organism
whole organism
organism part
replicate 2
replicate
Staphylococcus aureus
scientific_name
LAC+pDB59
strain
Sample 2
title