2024-03-07
ENA first public
ERC000011
ENA-CHECKLIST
Wellcome Sanger Institute
INSDC center name
public
INSDC status
ERS18399652
SRA accession
E-MTAB-13874_2:5386STDY7600834
Submitter Id
3
month
age
ArrayExpress
broker name
cell
cell_type
young
cohort
not collected
collection date
house mouse
common name
Protocols: Three 19 months old and five 3 months old male mice of C57BL/6JRj strain were obtained from Janvier labs, France.They were used to dissect hindlimb muscles for the single-cell isolation according to the following protocol https://www.protocols.io/view/single-cell-isolation-from-human-skeletal-muscle-261geyydv479/v1. Briefly, muscle tissue was minced, digested in the solution of Collagenase II (Worthington-Biochem, LS004176) and Dispase (Gibco, 17105041) and, following that, the lysate was centrifuged in the gradient of Percoll to recover a fraction with single-cells. Cell or nuclei suspension were  loaded on the Chromium Controller (10X Genomics) with a targeted cell or nuclei recovery of 8000.  10X Genomics Single Cell 3’ v2 or v3 Reagent Kit was used to create GEM, perform cDNA synthesis and generate sequencing libraries according to standard instructions.
description
adult
developmental stage
not collected
geographic location (country and/or sea)
Mouse7
individual
none
injury
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
hindlimb muscle
organism part
Mus musculus
scientific_name
male
sex
5386STDY7600834
title