2024-03-07
ENA first public
ERC000011
ENA-CHECKLIST
Wellcome Sanger Institute
INSDC center name
public
INSDC status
ERS18399677
SRA accession
E-MTAB-13874_2:mus_SNuc7503536
Submitter Id
year
age
ArrayExpress
broker name
nuclei
cell_type
young
cohort
not collected
collection date
house mouse
common name
Protocols: Three 19 months old and five 3 months old male mice of C57BL/6JRj strain were obtained from Janvier labs, France.They were used to dissect hindlimb muscles for the single-nucleus isolation according to the following protocol: https://www.protocols.io/view/nuclei-isolation-from-human-skeletal-muscle-kqdg34pqel25/v1. Briefly, muscle tissue was grounded using a dounce homogenizer, lysed in the nuclei lysis buffer and, finally, Percoll gradient was used to separate intact nuclei for loading and cell debris. Cell or nuclei suspension were  loaded on the Chromium Controller (10X Genomics) with a targeted cell or nuclei recovery of 8000.  10X Genomics Single Cell 3’ v2 or v3 Reagent Kit was used to create GEM, perform cDNA synthesis and generate sequencing libraries according to standard instructions.
description
adult
developmental stage
not collected
geographic location (country and/or sea)
Mouse2
individual
normal
injury
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
hindlimb muscle
organism part
Mus musculus
scientific_name
male
sex
mus_SNuc7503536
title