2024-03-07
ENA first public
ERC000011
ENA-CHECKLIST
Wellcome Sanger Institute
INSDC center name
public
INSDC status
ERS18399691
SRA accession
E-MTAB-13874_2:WS_A_SKM9426500
Submitter Id
25-30
year
age
ArrayExpress
broker name
nuclei
cell_type
young
cohort
not collected
collection date
human
common name
Protocols: Human intercostal muscle biopsies (inner part between the second and third ribs) were collected with consent from deceased transplant organ donors by the Cambridge Biorepository for Translational Medicine, Cambridge, UK (CBTM), immediately placed in HypoThermosol FRS preservation solution and shipped to Sanger Institute for processing.  Skeletal muscle tissue was processed according to the following protocol for single-nucleus isolation: https://www.protocols.io/view/nuclei-isolation-from-human-skeletal-muscle-kqdg34pqel25/v1. Briefly, muscle tissue was grounded using a dounce homogenizer, lysed in the nuclei lysis buffer and, finally, Percoll gradient was used to separate intact nuclei for loading and cell debris. Cell or nuclei suspension were  loaded on the Chromium Controller (10X Genomics) with a targeted cell or nuclei recovery of 8000.  10X Genomics Single Cell 3’ v2 or v3 Reagent Kit was used to create GEM, perform cDNA synthesis and generate sequencing libraries according to standard instructions.
description
adult
developmental stage
not collected
geographic location (country and/or sea)
566B
individual
Intracranial haemorrhage
injury
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
intercostal muscle
organism part
Homo sapiens
scientific_name
female
sex
WS_A_SKM9426500
title