2024-03-08
ENA first public
ERC000011
ENA-CHECKLIST
UMC Utrecht
INSDC center name
public
INSDC status
ERS18345023
SRA accession
E-MTAB-13842:H40N.2
Submitter Id
ArrayExpress
broker name
colonic epithelium cell line
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: Organoids were collected from Matrigel by enzymatic digestion using 1 mg/ml dispase for 10 minutes at 37°C and washed 2 times in 1xPBS. Organoids were lysed in RLT-buffer (Qiagen) supplemented with 1% β-mercaptoethanol and were stored at -80C. All organoids were grown in the presence of WNT surrogate/R-spondin/EGF-supplemented medium for 7 days, after which RNA was isolated. Total RNA was extracted using the QIAGEN RNeasy Plus Micro kit as per manufacturer’s instructions. DNase (Qiagen) treatment was included to avoid possible genomic DNA contamination. RNA was eluted in 30 μl of RNAse free water. Libraries were prepared using Truseq RNA stranded polyA (Illumina) according to the manufacturer’s directions.
description
adult
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
colorectum
organism part
Homo sapiens
scientific_name
H40N.2
title