2024-03-10
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Immunology and Hematology
INSDC center name
public
INSDC status
ERS18370994
SRA accession
E-MTAB-13864:GPVI-WT-E22-10
Submitter Id
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Samples were 10m thin slides of paraffine embedded lungs stocked at room temperature.  NucleoSpin totalRNA FFPE XS Micro kit (Cat.740969.50, Macherey-Nagel) was used according to manufacturer’s instruction and RNA was eluted in 30μg/mL DNaseI under gentle mixing at 37°C for 10L final volume. RNA concentration was assessed after extraction at the NanoPhotometer® N60 (Implemen). RNA integrity (>50% DV200) was assessed by Bioanalyzer (total RNA Pico Kit and RNA 6000 Nano Kit, 2100 Instrument, Agilent Technologies, Paolo Alto, CA, USA). Sequencing libraries were prepared using “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina“ combined with “NEBNext rRNA Depletion Kit v2” for ribosomal RNA depletion (New England Biolabs, Ipswich, MA).
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
lung
organism part
Mus musculus
scientific_name
GPVI-WT-E22-10
title