2024-03-12
ENA first public
ERC000011
ENA-CHECKLIST
Genentech
INSDC center name
public
INSDC status
ERS18296866
SRA accession
E-MTAB-13815:EO771_Trex1 KO_3
Submitter Id
ArrayExpress
broker name
E0771
cell_line
not collected
collection date
house mouse
common name
Protocols: Cultured cell lines were spun into cell pellets were snap frozen. Snap frozen cell pellets from cultured cell lines were directly lysed with RLT lysis buffer of the Rneasy Plus Mini kit (Qiagen).  Total RNA was quantified with the Qubit RNA HS Assay Kit (Thermo Fisher Scientific) and quality was assessed using RNA ScreenTape on a TapeStation 4200 (Agilent Technologies). For sequencing library generation, the TruseqStranded mRNA kit (Illumina) was used with an input of 100-1000 nanograms of total RNA. Libraries were quantified with Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and the average library size was determined using D1000 ScreenTape on TapeStation 4200 (Agilent Technologies).
description
Trex1 KO
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Mus musculus
scientific_name
EO771_Trex1 KO_3
title