2024-03-12
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115120805
External Id
Epigenetics and Development Division, The Walter and Eliza Hall Institute of Medical Research
INSDC center name
2024-01-19T17:58:44Z
INSDC last update
public
INSDC status
ERS17768975
SRA accession
E-MTAB-13706:placenta_2
Submitter Id
E14.5
day
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: The embryonic portion of F1 B6Cast placentae at E14.5 was dissected from the maternal portion in phosphate buffered saline before snap-freezing in liquid nitrogen. DNA extraction from E14.5 placentae was performed using the AllPrep DNA/RNA Mini Kit (Qiagen), according to manufacturer’s instructions. Samples were sexed by polymerase chain reaction (PCR) using primers for Otc (X-linked gene) and Zfy (Y-linked gene) as previously described and female samples were selected for further analysis. Placenta libraries were prepared with the ligation kit SQK-LSK109 (Oxford Nanopore Technologies).
description
mouse embryo stage
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
656-2
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
placenta
organism part
Mus musculus
scientific_name
female
sex
B6CAST F1
strain
placenta_2
title