2024-03-15
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA14321842
External Id
Instituto de Quimica Biologica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires, Argentina
INSDC center name
2022-05-04T15:27:56Z
INSDC last update
public
INSDC status
ERS11915962
SRA accession
E-MTAB-11689:P_extremaustralis_M_GSNO_B
Submitter Id
ArrayExpress
broker name
S-nitrosoglutathione
compound
Protocols: P. extremaustralis 14-3b, a species isolated from the Antarctic continent, was used through the experiments. Bacterial cultures were grown in Lysogeny Broth medium. (LB) at 30 °C. Microaerobic (or low aeration) cultures were incubated in sealed bottles with a 1:2 medium-to-flask volume ratio and 50 rpm agitation. Microaerobic culture’s medium was supplemented with 0.08% KNO3. For stress experiments, cultures were microaerobically grown for 24 h and then incubated for 1 h with 100 µM S-nitrosoglutatione (GSNO) (m-NS conditions). The concentration and exposure time were selected based on growth and survival analysis. Total RNA was isolated from P. extremaustralis control and treated samples cultures using the Trizol method. Samples were treated with DNAse I. Samples were validated using an Agilent 2100 Bioanalyzer (Agilent Technologies). To improve the quality of the readings, ribosomal RNA was depleted from the samples, using the RiboZERO Kit (Illumina), following the manufacturer's instructions. ibraries were prepared using the TruSeq RNA Library Prep Kit v2 (Illumina).
description
100
micromolar
dose
wild type genotype
genotype
microaerobiosis in media supplemented with 0.08% KNO6
growth condition
not applicable
isolate
Pseudomonas extremaustralis
organism
Pseudomonas extremaustralis
organism
whole organism
organism part
Pseudomonas extremaustralis
scientific_name
14-3b
strain
24
hour
time
P_extremaustralis_M_GSNO_B
title