2024-03-16
ENA first public
ERC000011
ENA-CHECKLIST
STANFORD UNIVERSITY
INSDC center name
public
INSDC status
ERS18401348
SRA accession
E-MTAB-13894:PeV-A2
Submitter Id
ArrayExpress
broker name
HT-29
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: >1000-fold coverage of mutagenized cells (Brunello library) was infected with PeV-A1 or PeV-A2 at MOI 0.1. Resistant cell colonies were harvested. Uninfected starting populations of mutagenized cells were used as the unselected reference. Total genomic DNA from both virus-resistant and uninfected cells was respectively extracted using QIAamp DNA Mini Kit (Qiagen). A first round of PCR was used to amplify the guide RNA sequences encoded in the gDNA, followed by a second round of PCR to add the barcodes/adapters for amplicon sequencing. 2% agarose gels and a QIAquick gel extraction kit were used to purify the amplicons.
description
Intestinal Type Adenocarcinoma
disease
lentiCas9-Blast; lentiGuide-Puro; SLC35A1/EXTL3 knockout; PeV-A2 infection
genotype
not collected
geographic location (country and/or sea)
PeV-A2
infect
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
colon
organism part
Homo sapiens
scientific_name
PeV-A2
title