2024-03-18
ENA first public
ERC000011
ENA-CHECKLIST
TVP Lab, WT/MRC Institute of Metabolic Science, University of Cambridge, United Kingdom
INSDC center name
public
INSDC status
ERS18444058
SRA accession
E-MTAB-12808_4:Sample 267
Submitter Id
-
week
age
108
milli-International Unit per milliliter
aspartate aminotransferase measurement
0
ballooning
33.1
gram
body weight
ArrayExpress
broker name
4.274
cholesterol
not collected
collection date
house mouse
common name
6J-CTRL-12W
comparison group name
Protocols: Murine tissues for protein or RNA extraction were frozen at the time of collection and stored at -80. Since the murine data are derived from multiple partners, different RNA extraction methods have been used (miRNAeasy Mini Kits; Qiagen, RNeasy, Trizol; Qiagen, STAT-60, Trizol reagent; thermofisher, LifeTech Magmax-96) according to the manufacturer’s instructions. All reagents, plastic ware, and supplies used were nuclease-free, sterile, and of molecular biology grade. RNA Integrity Number (RIN) of 8 was considered the lowest cut-off for RNA sequencing. RNA from tissues (1 ug RNA) were used to generate barcoded sequencing libraries using Illumina TruSeq® Stranded mRNA Library Preparation Kit (Illumina) following manufacturer’s instructions.
description
-
developmental stage
CDAHFD45_vs_REV
diet group
non-alcoholic fatty liver disease
disease
NAFLD
disease staging
12
week
duration of experimental diet
2
fibrosis
WT
genotype
not collected
geographic location (country and/or sea)
-
individual
2
inflammation
not applicable
isolate
4.07
liver weight to body weight (%)
6J−CDAHFD−F45−4W−REV−8W
model name
3
nas score
Mus musculus
organism
Mus musculus
organism
Liver
organism part
Mus musculus
scientific_name
55
milli-International Unit per milliliter
serum alanine aminotransferase measurement
male
sex
1
steatosis
C57BL/6J
strain
Sample 267
title
1.446
triglyceride