2024-03-18
ENA first public
ERC000011
ENA-CHECKLIST
TVP Lab, WT/MRC Institute of Metabolic Science, University of Cambridge, United Kingdom
INSDC center name
public
INSDC status
ERS18443901
SRA accession
E-MTAB-12808_4:Sample 125
Submitter Id
-
week
age
55.9
milli-International Unit per milliliter
aspartate aminotransferase measurement
1
ballooning
511.8
gram
body weight
ArrayExpress
broker name
NA
cholesterol
not collected
collection date
Norway rat
common name
R-CDAA-8W, R-CDAA-C2-8W
comparison group name
Protocols: Murine tissues for protein or RNA extraction were frozen at the time of collection and stored at -80. Since the murine data are derived from multiple partners, different RNA extraction methods have been used (miRNAeasy Mini Kits; Qiagen, RNeasy, Trizol; Qiagen, STAT-60, Trizol reagent; thermofisher, LifeTech Magmax-96) according to the manufacturer’s instructions. All reagents, plastic ware, and supplies used were nuclease-free, sterile, and of molecular biology grade. RNA Integrity Number (RIN) of 8 was considered the lowest cut-off for RNA sequencing. RNA from tissues (1 ug RNA) were used to generate barcoded sequencing libraries using Illumina TruSeq® Stranded mRNA Library Preparation Kit (Illumina) following manufacturer’s instructions.
description
-
developmental stage
CTRL
diet group
Healthy
disease
CTRL
disease staging
8
week
duration of experimental diet
0
fibrosis
WT
genotype
not collected
geographic location (country and/or sea)
-
individual
0
inflammation
not applicable
isolate
3.01
liver weight to body weight (%)
R-CTRL-8W
model name
1
nas score
Rattus norvegicus
organism
Rattus norvegicus
organism
Liver
organism part
Rattus norvegicus
scientific_name
24.3
milli-International Unit per milliliter
serum alanine aminotransferase measurement
male
sex
0
steatosis
RjHan:Wi
strain
Sample 125
title
NA
triglyceride