2024-03-18
ENA first public
ERC000011
ENA-CHECKLIST
Department of Botany and Nature Protection, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn
INSDC center name
public
INSDC status
ERS18108286
SRA accession
E-MTAB-13773:3_15-16_AP13
Submitter Id
8
month
age
ArrayExpress
broker name
not collected
collection date
pig
common name
Protocols: The in vitro cultures of the endometrial tissue explants were preincubated (2 h) and incubated for 24 h in the phenol-red free medium M199 (Sigma-Aldrich, USA; 37°C, 95% O2, 5% CO2). The tissues descended to the control groups were incubated with the medium alone, whereas the tissues from the adipokine-treated group were incubated in the presence of apelin-13 at the dose of 20 ng/mL. The in vitro incubations were run in four separate experiments (n = 4, one animal for each experiment) in duplicates. The activity of lactate dehydrogenase (LDH) after preincubation and incubation was used to determine the viability of the endometrial tissue explants. The isolation and cleaning-up processes for total RNA were performed using RNeasy Kit and RNase-Free DNase Set (Qiagen, Germany), respectively. The Quality and quantity of the obtained RNA determined using Tecan Infinite M200 reader (Tecan Group Ltd., Switzerland). Subsequently, all samples were subtracted to the analysis of the RNA integrity number (RIN) Agilent Bioanalyzer 2100 (Agilent Technology, USA). RNA samples with RINs in the range of 8–10 were used for the transcriptome high-throughput sequencing (RNA-Seq) and subsequent quantitative real-time PCR (qPCR) validations. Double-stranded cDNA libraries from RNA samples were performed with the use of Illumina Truseq Standard mRNA LT Sample Prep Kit (Illumina, USA). After the fragmentation of RNA samples, poly(dT) oligonucleotides were used in the process of the strand-specific reverse transcription into cDNA. The obtained cDNA was processed to 3&#39; tail adenylation and adapter ligation.
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
endometrial tissue explant
growth condition
3
individual
not applicable
isolate
Sus scrofa
organism
Sus scrofa
organism
uterus
organism part
Sus scrofa
scientific_name
female
sex
Large White × Polish Landrance
strain
3_15-16_AP13
title