2024-03-19
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114511299
External Id
Institut Pasteur, Biomics Platform
INSDC center name
2023-10-18T15:45:37Z
INSDC last update
public
INSDC status
ERS16492648
SRA accession
E-MTAB-13460:delta1847_BSplus4
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Overnight cultures were mixed with RNAprotect (Qiagen) in the anaerobic chamber to prevent RNA degradation and bacteria were lysed using QIAGEN Proteinase K and TE buffer containing lysozyme. Total RNA was extracted using the Direct Zol kit (Zymo Cat. R2050) according to the manufacturer’s instructions and treated with DNase I from the same kit. RNA concentration, quality, and integrity from 4 independent replicates was checked using RNA6000 Nano chips and the Agilent 2100 Bioanalyzer system ribozero plus was used for ribosomal RNA depletion
description
delta plus
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Bacteroides thetaiotaomicron
organism
Bacteroides thetaiotaomicron
organism
whole organism
organism part
Bacteroides thetaiotaomicron
scientific_name
delta1847_BSplus4
title