2024-03-21
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA112868550
External Id
NTU
INSDC center name
2023-04-04T14:46:56Z
INSDC last update
public
INSDC status
ERS14875205
SRA accession
E-MTAB-12834:Sample 2
Submitter Id
ArrayExpress
broker name
brown fat cell
cell_type
house mouse
common name
Protocols: Cultured cells were lysed in TRI reagent. RNA was isolated using TRI reagent with additional clean-up using a silica column-based isolation method (ISOLATE II RNA Mini Kit #BIO-52071, Bioline), including DNAse treatment. The RNA integrity of each sample was tested using Agilent 2100 Bioanalyser (RNA Nano (5-500 ng/μL)-Chip service) and samples with RNA Integrity Number (RIN) above 7.5 were deemed suitable for sequencing. 1)mRNA enrichment and purification: rRNA depletion or Oligo dT Selection to enrich the mRNA 2)RNA fragment and reverse transcription 3)End repair, add A and adaptor ligation 4)RCR amplification 5)Single strand separation and cyclization 6)DNA nanoball synthesis 7)Sequencing on BGISEQ platform
description
adult
developmental stage
normal
disease
wild type genotype
genotype
cultured in vitro
growth condition
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
brown adipose tissue
organism part
Mus musculus
scientific_name
Sample 2
title