2024-03-21
ENA first public
ERC000015
ENA-CHECKLIST
SAMEA13601223
External Id
Peking university
INSDC center name
2022-03-21T14:17:18Z
INSDC last update
public
INSDC status
ERS11203412
SRA accession
ena-SAMPLE-TAB-21-03-2022-14:17:16:233-98114
Submitter Id
2020-04-01
collection date
human
common name
A multi-center study was conducted collecting fecal samples (n=37, 29 patients and 8 volunteers) from April 2020 to December 2054 from and microbiome analyses were conducted from cardiac arrest patients
description
animal litter
environment (biome)
human gut
environment (feature)
nitrogen cycling
environment (material)
China
geographic location (country and/or sea)
39.4
DD
geographic location (latitude)
115.7
DD
geographic location (longitude)
human-gut
human gut environmental package
metagenome
investigation type
Homo sapiens
organism
Gut is associated with outcomes in cardiac arrest patients:a retrospective cohort study
project name
Homo sapiens
scientific_name
Gut is associated with outcomes in cardiac arrest patients:a retrospective cohort study sequencing method Fecal matter was used for DNA extraction (QIAamp PowerFecal Pro DNA Kit). DNA library preparation was conducted according to the manufacturer��s instructions using KAPA HyperPlus PCR-free (96rxn), and qualified DNA sequences were divided into fragments of approximately 350 bp. Manufactured dual-indexed adapters with sequences were duplicated in-house. Indexed adapter ligation was performed using an adapter. After ligation, the DNA libraries were purified and amplificated. The libraries were quantified with Qubit2.34 using Q-PCR, and the pool was sequenced with Illumina HiSeq.
sequencing method
IMRK
title