2024-03-21
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114501083
External Id
Gyeongsang National University Animal Genomics Laboratory
INSDC center name
2023-10-16T16:10:00Z
INSDC last update
public
INSDC status
ERS16482735
SRA accession
E-MTAB-13398:192031
Submitter Id
22
month
age
520
kilogram
body weight
ArrayExpress
broker name
not collected
collection date
cattle
common name
Protocols: To identify expression quantitative trait loci (eQTL) in Hanwoo cattle, 32 animals were provided by the Hanwoo cattle Research institute, National Institute of Animal Science, South Korea. For RNA-sequencing, tissue samples, such as Abdominal Fat (ABF), Back Fat (BFT) and Kidney Fat (KIF) were collected from the castrated Hanwoo cattle after slaughtering at 10~26 months of age. Ethics approval was obtained from the National Institute of Animal Science (approval no: NIAS20201979). Total RNA was extracted from the trachea with TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocol. RNA concentration was checked using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, USA). Individual libraries were generated using Illumina TruSeq™ RNA Sample Preparation Kit (Illumina, San Diego, CA, USA).
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Bos taurus
organism
Bos taurus
organism
Back fat
organism part
case
phenotype
Bos taurus
scientific_name
castrated male
sex
Hanwoo cattle (Korean native cattle)
strain
192031
title