2024-03-21
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA112878197
External Id
UOE
INSDC center name
2023-04-05T14:39:07Z
INSDC last update
public
INSDC status
ERS14884852
SRA accession
E-MTAB-12748:iSAM NT -Dox GEX 5
Submitter Id
ArrayExpress
broker name
SFCi55
cell_line
hematopoietic cell
cell_type
human
common name
Protocols: The cells were sorted by FACS as CD34+ live and singlet. hPSCs were differentiated in a xeno-free composition of SFD medium 14, BSA was substituted with human serum albumin, HSA, (Irvine-Scientific). Day 0 differentiation medium, containing 10 ng/ml BMP4 was added to the colonies prior cutting. Cut colonies were transferred to a bacteriological grade well to form embryoid bodies and cultured for two days. At day 2 media was changed and supplemented with 3 uM CHIR (StemMacs). At day 3, medium was changed and supplemented with 5 ng/ml bFGF and 15 ng/ml VEGF. At day 6 media was changed for final haematopoietic induction in SFD medium supplemented with 5 ng/ml bFGF, 15 ng/ml VEGF, 30 ng/ml IL3, 10 ng/ml IL6, 5 ng/ml IL11, 50 ng/ml SCF, 2 U/ml EPO, 30 ng/ml TPO, 10 ng/ml FLT3L and 25 ng/ml IGF1. From day 6 onward, cytokines were replaced every two days. According to 10x manufacturer protocol According to 10x manufacturer protocol
description
adult
developmental stage
normal
disease
embryoid body 10 days after differentiation
growth condition
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
skin
organism part
induced pluripotent stem cell
progenitor cell type
Homo sapiens
scientific_name
iSAM NT -Dox GEX 5
title