2024-03-21
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA112868546
External Id
NTU
INSDC center name
2023-04-04T14:46:56Z
INSDC last update
public
INSDC status
ERS14875201
SRA accession
E-MTAB-12834:Sample 7
Submitter Id
ArrayExpress
broker name
brown fat cell
cell_type
house mouse
common name
Protocols: Cultured cells were lysed in TRI reagent. RNA was isolated using TRI reagent with additional clean-up using a silica column-based isolation method (ISOLATE II RNA Mini Kit #BIO-52071, Bioline), including DNAse treatment. The RNA integrity of each sample was tested using Agilent 2100 Bioanalyser (RNA Nano (5-500 ng/μL)-Chip service) and samples with RNA Integrity Number (RIN) above 7.5 were deemed suitable for sequencing. 1)mRNA enrichment and purification: rRNA depletion or Oligo dT Selection to enrich the mRNA 2)RNA fragment and reverse transcription 3)End repair, add A and adaptor ligation 4)RCR amplification 5)Single strand separation and cyclization 6)DNA nanoball synthesis 7)Sequencing on BGISEQ platform
description
adult
developmental stage
normal
disease
Tlr4 -/-
genotype
cultured in vitro
growth condition
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
brown adipose tissue
organism part
Mus musculus
scientific_name
Sample 7
title