2024-03-29
ENA first public
ERC000011
ENA-CHECKLIST
MRC Centre for Neurodevelopmental Disorders, Institute of psychiatry, psychology, & Neuroscience, King's College London.
INSDC center name
public
INSDC status
ERS18400119
SRA accession
E-MTAB-13886:Foxg1_null_6
Submitter Id
ArrayExpress
broker name
not collected
collection date
zebrafish
common name
Protocols: Heterozygous mutant (5bp deletion 32 bp from canonical start codon; AAATG) foxg1a adult zebrafish in a dlx5-6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous (no interneurons in telencephalon) and heterozygous (reduced number of interneurons in telencephalon). Genotyping was confirmed with RNA-sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit (Qiagen, 74004). RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo-Zero Gold.
description
pharyngula prim-5
developmental stage
Foxg1a hom
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Danio rerio
organism
Danio rerio
organism
whole organism
organism part
Danio rerio
scientific_name
Foxg1_null_6
title