2024-03-30
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113399738
External Id
Quadram Institute Bioscience
INSDC center name
2023-05-03T11:00:52Z
INSDC last update
public
INSDC status
ERS15394885
SRA accession
E-MTAB-12851:HG2_BG_Ctrl_2
Submitter Id
ArrayExpress
broker name
HepG2
cell_line
hepatocyte
cell_type
human
common name
Protocols: HepG2 cells were seeded on a 6-well plate (2x10^5 cells/well). On the day of the treatment, the media was removed and the wells washed twice with 1X DPBS (14190144 Gibco) and 10 µM of SF or control (DMSO) in serum-free DMEM without glucose (Catalogue: 11966025 Gibco) supplemented with 1 mM sodium pyruvate (11360070 Gibco), and 5.5 mM or 25 mM glucose (A2494001 Gibco) For the continuous SF treatment RNA was extracted at 24 h. After 24 h, for all experimental procedures, following the desired time point: 1) Media was removed from six well plate, 2) cells were washed twice with 1XDPBS, 3) Buffer RLT with β-ME (444203 Sigma), to control and sulforaphane treated cells, were lysed directly on the 6-well plate. RNA was then extracted using the RNeasy Qiagen kit (Ref:74004) according to the manufacturer's instructions. RNA abundance and integrity were assessed using the Nanodrop ND-1000 spectrophotometer. Library preparation (TruSeq RNA Poly-A) was performed by Macrogen
description
adult
developmental stage
hepatocellular carcinoma
disease
wild type genotype
genotype
Homo sapiens
organism
Homo sapiens
organism
liver
organism part
Homo sapiens
scientific_name
HG2_BG_Ctrl_2
title