2024-03-30
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113405132
External Id
EMBL
INSDC center name
2023-05-05T11:30:02Z
INSDC last update
public
INSDC status
ERS15400263
SRA accession
E-MTAB-12981:D10_TR
Submitter Id
ArrayExpress
broker name
house mouse
common name
Protocols: Embryos were generated through IVF, microinjected, cultured in a standard mammalian cell incubator in KSOM medium until 2-cell stage and then transferred to pseudopregnant females for in vivo growth until E7. Extraction of RNA from single E7 embryonic halves using Monarch Total RNA Miniprep Kit (NEB). Modified Smart-Seq2 protocol using SuperScript IV RT and tagmentation procedure previously described (Hennig et al. 2018).
description
embryonic day 7
developmental stage
normal
disease
wild type genotype
genotype
D10
individual
Mus musculus
organism
Mus musculus
organism
trophectoderm
organism part
frozen_sample
sample with known storage state
Mus musculus
scientific_name
male
sex
FVB/NcrlxPWD/Ph
strain
D10_TR
title
dBtgh_injected
treatment