2024-04-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114857229
External Id
Faculty of Biology - Technion Israel Institute of Technology
INSDC center name
2023-12-07T22:46:10Z
INSDC last update
public
INSDC status
ERS17282188
SRA accession
E-MTAB-13617:HCC827_siRBM10_A
Submitter Id
ArrayExpress
broker name
HCC827
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: Cells were plated at 6cm cells and 24 hours later transfected with control or RBM10 siRNA. 48 hours after siRNA transfection, cells were directly lysed using TRIzol reagent (Ambion) for RNA extraction. Cells were grown in a humidified 37C incubator (5% CO2) in RPMI-1640 medium (Gibco) supplemented with 10% heat-inactivated FBS, 2mM L-glutamine (Gibco), 100unit/mL penicillin and 100μg/mL streptomycin (Gibco). siRNA transfections were performed using Lipofectamine™ RNAiMAX (Invitrogen) according to the manufacturer's instructions. Total RNA was extracted from cells using TRIzol reagent according to the manufacturer's instructions (Ambion). RNA-seq libraries were prepared at the Crown Genomics institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science using TruSeq mRNA library preparation kit (Illumina). Libraries were quantified by Qubit (Thermo fisher scientific) and TapeStation (Agilent).
description
adult
developmental stage
lung adenocarcinoma
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
lung
organism part
Homo sapiens
scientific_name
HCC827_siRBM10_A
title