2024-04-01
ENA first public
ERC000011
ENA-CHECKLIST
Faculty of Biotechnology, University of Wrocław
INSDC center name
public
INSDC status
ERS18433037
SRA accession
E-MTAB-13911:JD04
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: RNA was isolated from Streptomyces venezeulae JD01 (cobbB1 deletion) or JD07 (cobB2 deletion) strains cultivated in 5 mL MYM liquid medium for 10 or 20 hours (each time point in duplicate). Mycelia were collected by centrifugation, frozen, and stored at -70°C for subsequent RNA isolation. RNA from mycelia was isolated using the GeneJET RNA isolation kit (Thermo Fisher Scientific, US). The lysozyme concentration in the suspension buffer was increased up to 10 mg/mL to improve the yield of mycelium lysis. The isolated RNA was digested with TURBO DNase I (Invitrogen, United States) and subsequently, purified and concentrated using a GeneJET RNA Cleanup kit (Thermo Fischer Scientific, US) RNA libraries were prepared for sequencing using standard Illumina protocols (NEBNext Ultra II Directional RNA Library Prep Kit). rRNA was removed using Ribo-Zero Ribosomal RNA Removal Kit (Illumina)
description
spores maturation
developmental stage
not collected
geographic location (country and/or sea)
not applicable
isolate
Streptomyces venezuelae
organism
Streptomyces venezuelae
organism
whole organism
organism part
2
replicate
Streptomyces venezuelae
scientific_name
JD01
strain
JD04
title