2024-04-01
ENA first public
ERC000011
ENA-CHECKLIST
University Paul Sabatier INSERM U1297
INSDC center name
public
INSDC status
ERS18458668
SRA accession
E-MTAB-13915:IP MRPS15 tuna 2
Submitter Id
ArrayExpress
broker name
AC16
cell_line
cardiomyocyte
cell_type
not collected
collection date
human
common name
Protocols: AC16 cells were cultured in two 150 mm dishes per point. Ten minutes prior to harvesting, cells were treated with cycloheximide at 100 mg/mL. Cells were washed twice with PBS at room temperature containing 100 mg/mL cycloheximide and harvested at 80% confluence with Trypsin. tunicamycin has been applied to the cells at a concentration of 2 microgramms per milliliter for 4h at 37°C Standard extraction with Trizol has been applied according manufacturer recommandations. Library has been constructed according internal Genewiz/azenta pipeline. (genewiz.com)
description
adult
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
heart
organism part
3
replicate
Homo sapiens
scientific_name
IP MRPS15 tuna 2
title