2024-04-01
ENA first public
ERC000011
ENA-CHECKLIST
Institut des Maladies Métaboliques et Cardiovasculaires U1297 Inserm/Université Toulouse III Paul Sabatier
INSDC center name
public
INSDC status
ERS18431984
SRA accession
E-MTAB-13907:N0_3
Submitter Id
ArrayExpress
broker name
primary cell line
cell_line
Human Dermal Endothelial Cell
cell_type
not collected
collection date
human
common name
Protocols: HDLEC were harvested from culture at 70-80% confluency by trysinization no particular treatment has been applied to the HDLEC cells RNA extraction was performed with a Biobasic EZ-10 Total RNA extraction kit (BS82322) according to manufacturer protocol RNA-seq paired-end libraries have been prepared according to Illumina’s protocol using the TruSeq Stranded Total RNA Gold library prep Kit (Illumina, San Diego, USA). Libraries quality was assessed using the HS NGS kit on the Fragment Analyzer (Agilent Technologies, Santa Clara, USA). Libraries were quantified by qPCR using the KAPA Library Quantification Kit (Roche, Basel, Switzerland) to obtain an accurate quantification.
description
neonate
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
skin of prepuce of penis
organism part
2
replicate
Homo sapiens
scientific_name
N0_3
title