2024-04-01
ENA first public
ERC000011
ENA-CHECKLIST
INM – Leibniz Institute for New Materials
INSDC center name
public
INSDC status
ERS18431536
SRA accession
E-MTAB-13905:D7a
Submitter Id
ArrayExpress
broker name
HEK293T
cell_line
not collected
collection date
human
common name
Protocols: 600,000 HEK-293T cells/well were seeded onto 6-well plates (Corning, catalog no.CORN3516) and 24 h later transfected with 600 µL transfection mix containing 3.750 µg total DNA and 12.375 µL PEI per well. Blue-light illumination (460 nm, 5 µmol m-2 s-1) was started 8 h after transfection for 24 h. Controls were kept in darkness. Cells were harvested 24 h after treatment using 1 mL TriFast™ (VWR, cat. no. 30-2010) per well. Total RNA was extracted according to the manufacturer’s protocol. RNA was further cleaned up using an RNeasy Mini Kit (Qiagen, cat. no. 74104) according to the manufacturer's protocol, including an on-column DNase I digest using 50 µL reactions of DNase I (New England Biolabs, cat. no. M0303). RNA integrity was verified at BGI Tech Solutions (Hong Kong) Co. using a Bioanalyzer (Agilent 2100 Bioanalyzer). Strand-Specific mRNA Library Preparation (DNBSEQ). BGI Protocol.
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
darkness
stimulus
D7a
title