2024-04-05
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114307754
External Id
Charles University, Faculty of Science Department of Cell Biology
INSDC center name
2023-08-29T10:43:38Z
INSDC last update
public
INSDC status
ERS16296756
SRA accession
E-MTAB-13305:V5
Submitter Id
ArrayExpress
broker name
not collected
collection date
fission yeast
common name
Protocols: Samples were prepared from 3 biological replicates. Two separate experiments were performed: 1) Cells were cultured to exponential phase in the complex YES medium with or without ammonium supplementation. 2) Cells were cultured to exponential phase in the complex YES medium and then treated with DMSO (control) or 20 μM cerulenin for 1 hour. For each sample, 10 mL were harvested (600 g, 2 min) and the cell pellet was flash-frozen. Total RNA was isolated using a hot acidic phenol method followed by phenol-chloroform extractions and precipitation (PMID: 12854975). Extracted RNA was treated with TURBO DNase (Thermo Fisher Scientific) and purified using Qiagen RNeasy columns. RNA quality was assessed on Agilent Bioanalyzer. The sequencing libraries were synthesized using KAPA mRNA HyperPrep Kit (Illumina platform) (Roche, KK8581).
description
cbf11DBM
genotype
not collected
geographic location (country and/or sea)
YES medium with ammonium chloride
growth condition
not applicable
isolate
Schizosaccharomyces pombe
organism
Schizosaccharomyces pombe
organism
whole organism
organism part
1
replicate
Schizosaccharomyces pombe
scientific_name
MP712
strain
V5
title