2024-04-05
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA6101072
External Id
JIC
INSDC center name
2019-10-18T17:05:33Z
INSDC last update
public
INSDC status
ERS3901757
SRA accession
setikv_mk_2
Submitter Id
thale cress
common name
22 degree, short-day light, 75% humidity. Genotype: setikv= SETI with inducible mutant AvrRps4 (KRVY135-138AAAA) (Ngou et al 2019 JXB). Treatment: mk=mock treatment, infiltrated with 0.1% DMSO (in water), harvested at 3.5 hours post infiltration (hpi). Replicate: 1st biological replicate (1/9). Fresh nuclei were extracted from mature leaves from plants which are 5-week old and sorted with flow cytometry (fluorescence-activated nuclei sorting), and tagmented with Tn5 from an Illumina Nextera kit. ATACseq library was amplified with custom-design and 9nt-space dual-index primers. After multiplexed with other samples are sequenced with four flow cells of Nextseq 500/550 high output kit (75 cycles), and demultiplexed with Illumina dcl2fastq.
description
Arabidopsis thaliana
organism
Arabidopsis thaliana
scientific_name
setikv_mk_2
title