2024-04-05
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114307693
External Id
Charles University, Faculty of Science Department of Cell Biology
INSDC center name
2023-08-29T10:12:05Z
INSDC last update
public
INSDC status
ERS16296695
SRA accession
E-MTAB-13303:A1
Submitter Id
ArrayExpress
broker name
not collected
collection date
fission yeast
common name
Protocols: Samples were prepared from 3 biological replicates of mga2Δ (MP815) and mga2Δcbf11Δ (MP836) cells. Cells were cultured to exponential phase in the complex YES medium (with or without ammonium supplementation), 10 mL were harvested (600 g, 2 min) and the cell pellet was flash-frozen. Total RNA was isolated using a hot acidic phenol method followed by phenol-chloroform extractions and precipitation (PMID: 12854975). Extracted RNA was treated with TURBO DNase (Thermo Fisher Scientific) and purified using Qiagen RNeasy columns. RNA quality was assessed on Agilent Bioanalyzer. The sequencing libraries were synthesized using KAPA mRNA HyperPrep Kit (Illumina platform) (Roche, KK8581).
description
mga2
genotype
not collected
geographic location (country and/or sea)
YES medium
growth condition
not applicable
isolate
Schizosaccharomyces pombe
organism
Schizosaccharomyces pombe
organism
whole organism
organism part
1
replicate
Schizosaccharomyces pombe
scientific_name
MP815
strain
A1
title