2024-04-05
ENA first public
ERC000011
ENA-CHECKLIST
University of Chicago
INSDC center name
public
INSDC status
ERS18593196
SRA accession
E-MTAB-13931:Control_5
Submitter Id
24
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Live CD11b+ Ly6Chi cells were FACS sorted from wildtype B6 mice into Takara RNA isolation sort buffer containing RNase inhibitor and 3' Primer II A (as per Takara instructions)  RNA was isolated from sorted cells using the Takara SMART-Seq mRNA LP kit according to manufacturer directions.  RNA libraries were constructed using Takara SMART-Seq mRNA LP kit and Takara unique dual index kit according to manufacturer directions. Libraries were quantified, then equal quantity of library was pooled before sequencing
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
5
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
spleen
organism part
Mus musculus
scientific_name
female
sex
C57BL6
strain
Control_5
title