2024-04-06
ENA first public
ERC000011
ENA-CHECKLIST
Klinik und Poliklinik für Innere Medizin II Klinikum rechts der Isar der Technischen Universität München
INSDC center name
public
INSDC status
ERS18601959
SRA accession
E-MTAB-13946:S059-CPI3K-AP-0-BR6
Submitter Id
10
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Pancreatic tissue was collected from 10 week old mice Acute pancreatitis was induced as published (Jensen et al., Gastroenterology 2005) Maxwell 16 Tissue LEV Total RNA Purification Kit was used for RNA extraction according to the manufacturer's instructions (Promega) Library preparation for bulk 3’-sequencing of poly(A)-RNA was done as described previously (Parekh et al., 2016) barcoded cDNA of each sample was generated with a Maxima RT polymerase (Thermo Fisher) using oligo-dT primer containing barcodes, unique molecular identifiers (UMIs) and an adapter. 5’ ends of the cDNAs were extended by a template switch oligo (TSO) and after pooling of all samples full-length cDNA was amplified with primers binding to the TSO-site and the adapter. cDNA was tagmented with the Nextera XT kit (Illumina) and 3’-end-fragments finally amplified using primers with Illumina P5 and P7 overhangs.
description
Pi3kCAH1047R
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
pancreas
organism part
Mus musculus
scientific_name
acute pancreatitis
stimulus
C57BL6 / FVB/129
strain
S059-CPI3K-AP-0-BR6
title