2024-04-12
ENA first public
ERC000021
ENA-CHECKLIST
Royal NIOZ
INSDC center name
public
INSDC status
ERS18415105
SRA accession
NIOZ197.121.116
Submitter Id
0.073
ammonium (mg/L)
Marine benthic biome [ENVO:01000024]
broad-scale environmental context
<0.1
chlorophyll (µg/L)
2015-03-13
collection date
0
m
depth
PBSeT incubation - benthic habitat - 7.5 months
description
<1
dissolved organic carbon (mg/L)
7.92
dissolved oxygen (mg/L)
-40
m
elevation
Marine sediment [ENVO:03000033]|Plastisphere [ENVO:06105106]
environmental medium
Field incubation, Time series
experimental factor
Mediterranean Sea
geographic location (country and/or sea)
42.5779
DD
geographic location (latitude)
10.1078
DD
geographic location (longitude)
Tyrrhenian Sea: Italy, Island of Pianosa, National Park Tuscan Archipelago
geographic location (region and locality)
Coastal zone [ENVO:00000486]
local environmental context
FWD (Golay121): TGTGCACGCCAT; REV (BC116): GTGAGGGCAAGT
multiplex identifiers
<0.02
nitrate (mg/L)
0.067
nitrite (mg/L)
Powersoil DNA Isolation kit
nucleic acid extraction
1.6
organic carbon (mg/L)
plastisphere metagenome
organism
8.22
pH
>0.6
particulate organic carbon (mg/L)
Initial denaturation: 98degC_30s; 30 cycles of denaturation: 98degC_10s, annealing: 50degC_15s, elongation: 72degC_30s; final elongation: 72degC_5min
pcr conditions
FWD (515F-Y): GTGYCAGCMGCCGCGGTAA; REV (926R): CCGYCAATTYMTTTRAGTTT
pcr primers
0.6
phaeopigments (µg/L)
<10
phosphate (mg/L)
0.1
polymer disintegration (%)
Open-Bio bioplastics
project name
38.1
psu
salinity
plastisphere metagenome
scientific_name
software
sequence quality check
Illumina MiSeq
sequencing method
0.21
silicate (mg/L)
16S/18S SSU rRNA
target gene
400bp, 600bp
targeted amplicon size
12.4
ºC
temperature
3A1B
title
33
total carbon (mg/L)
<0.04
total nitrogen (mg/L)