2024-04-12
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114328066
External Id
Max Planck Unit for the Science of Pathogens
INSDC center name
2023-09-05T10:04:52Z
INSDC last update
public
INSDC status
ERS16317176
SRA accession
SF370_pEC2813_rep3
Submitter Id
Max Planck Unit for the Science of Pathogens
collected_by
2020-06-17
collection date
C medium
culture_medium
S. pyogenes SF370 was transformed with the tadA overexpression plasmid pEC2813, and transformants were selected on TSA plates with 3% sheep blood and 300 �g/mL kanamycin. Overnight cultures in C medium with 300 �g/mL kanamycin were inoculated with single colonies, grown overnight, and diluted to an OD620 of 0.02. Cultures were grown at 37 degree C and 5% CO2, and culture aliquots were mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v) at mid-logarithmic growth phase (ML; OD620 of 0.25). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.
description
wild type
genotype
Germany
geographic location (country and/or sea)
Streptococcus pyogenes M1 GAS
organism
Streptococcus pyogenes M1 GAS
scientific_name
M1
serotype (required for a seropositive sample)
SF370
strain
S. pyogenes SF370 with tadA-overexpressing pEC2813, mid-logarithmic growth phase in C medium, replicate 3
title