2024-04-12
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114328058
External Id
Max Planck Unit for the Science of Pathogens
INSDC center name
2023-09-05T10:04:52Z
INSDC last update
public
INSDC status
ERS16317168
SRA accession
SF370_ES_rep1
Submitter Id
Max Planck Unit for the Science of Pathogens
collected_by
2018-10-09
collection date
C medium
culture_medium
Overnight cultures of S. pyogenes SF370 were diluted to an OD620 of 0.02 in C medium and grown at 37 degree C and 5% CO2. At early stationary growth phase (ES; OD620 of 0.4), an aliquot of the culture was removed and mixed with an equal volume of ice-cold acetone:ethanol (1:1, v/v). Cells were pelleted, washed with TE-sucrose (50 mM Tris-HCl, 10 mM EDTA, 50 mM NaCl, 25% sucrose; pH 8.0) and stored at -80 degree C.
description
wild type
genotype
Germany
geographic location (country and/or sea)
Streptococcus pyogenes M1 GAS
organism
Streptococcus pyogenes M1 GAS
scientific_name
M1
serotype (required for a seropositive sample)
SF370
strain
S. pyogenes SF370, early stationary growth phase in C medium, replicate 1
title