2024-04-12
ENA first public
ERC000024
ENA-CHECKLIST
Royal NIOZ
INSDC center name
public
INSDC status
ERS18414424
SRA accession
NIOZ197.017.012
Submitter Id
<0.04
ammonium (mg/L)
Mediterranean Sea Biome [ENVO:01000047]
broad-scale environmental context
<1
chlorophyll (µg/L)
2014-10-07
collection date
20
m
depth
LDPE incubation - pelagic habitat - 2.5 months
description
2.7
dissolved organic carbon (mg/L)
7.14
dissolved oxygen (mg/L)
Sea Water [ENVO:00002149]|Plastisphere [ENVO:06105106]
environmental medium
Field incubation, Time series
experimental factor
Mediterranean Sea
geographic location (country and/or sea)
42.5779
DD
geographic location (latitude)
10.1078
DD
geographic location (longitude)
Tyrrhenian Sea: Italy, Island of Pianosa, National Park Tuscan Archipelago
geographic location (region and locality)
Coastal zone [ENVO:00000486]
local environmental context
FWD (Golay17): GACTCAACCAGT; REV (BC12): CCTGCACCTGCA
multiplex identifiers
0.04
nitrate (mg/L)
<0.02
nitrite (mg/L)
Powersoil DNA Isolation kit
nucleic acid extraction
not collected
organic carbon (mg/L)
plastisphere metagenome
organism
8.2
pH
not collected
particulate organic carbon (mg/L)
Initial denaturation: 98degC_30s; 30 cycles of denaturation: 98degC_10s, annealing: 50degC_15s, elongation: 72degC_30s; final elongation: 72degC_5min
pcr conditions
FWD (515F-Y): GTGYCAGCMGCCGCGGTAA; REV (926R): CCGYCAATTYMTTTRAGTTT
pcr primers
<1
phaeopigments (µg/L)
<0.01
phosphate (mg/L)
0.05
polymer disintegration (%)
Open-Bio bioplastics
project name
38
psu
salinity
plastisphere metagenome
scientific_name
software
sequence quality check
Illumina MiSeq
sequencing method
<2
silicate (mg/L)
16S/18S SSU rRNA
target gene
400bp, 600bp
targeted amplicon size
21
ºC
temperature
1C3P
title
40
m
total depth of water column
not collected
total inorganic nitrogen (mg/L)
<0.25
total nitrogen (mg/L)
<0.01
total phosphorus (mg/L)