2024-04-12
ENA first public
ERC000021
ENA-CHECKLIST
Royal NIOZ
INSDC center name
public
INSDC status
ERS18415102
SRA accession
NIOZ197.115.110
Submitter Id
0.11
ammonium (mg/L)
Marine benthic biome [ENVO:01000024]
broad-scale environmental context
<1
chlorophyll (µg/L)
2014-12-22
collection date
0
m
depth
LDPE incubation - benthic habitat - 5 months
description
2
dissolved organic carbon (mg/L)
7.46
dissolved oxygen (mg/L)
-40
m
elevation
Marine sediment [ENVO:03000033]|Plastisphere [ENVO:06105106]
environmental medium
Field incubation, Time series
experimental factor
Mediterranean Sea
geographic location (country and/or sea)
42.5779
DD
geographic location (latitude)
10.1078
DD
geographic location (longitude)
Tyrrhenian Sea: Italy, Island of Pianosa, National Park Tuscan Archipelago
geographic location (region and locality)
Coastal zone [ENVO:00000486]
local environmental context
FWD (Golay115): ACTTGGTGTAAG; REV (BC110): AGAGGAGTCGAC
multiplex identifiers
0.043
nitrate (mg/L)
<0.02
nitrite (mg/L)
Powersoil DNA Isolation kit
nucleic acid extraction
not collected
organic carbon (mg/L)
plastisphere metagenome
organism
8.1
pH
not collected
particulate organic carbon (mg/L)
Initial denaturation: 98degC_30s; 30 cycles of denaturation: 98degC_10s, annealing: 50degC_15s, elongation: 72degC_30s; final elongation: 72degC_5min
pcr conditions
FWD (515F-Y): GTGYCAGCMGCCGCGGTAA; REV (926R): CCGYCAATTYMTTTRAGTTT
pcr primers
2.4
phaeopigments (µg/L)
<10
phosphate (mg/L)
0
polymer disintegration (%)
Open-Bio bioplastics
project name
38.2
psu
salinity
plastisphere metagenome
scientific_name
software
sequence quality check
Illumina MiSeq
sequencing method
<2
silicate (mg/L)
16S/18S SSU rRNA
target gene
400bp, 600bp
targeted amplicon size
17.3
ºC
temperature
2C1B
title
22
total carbon (mg/L)
<0.25
total nitrogen (mg/L)