2024-04-13
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA112486882
External Id
Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany
INSDC center name
2023-02-08T11:12:34Z
INSDC last update
public
INSDC status
ERS14604214
SRA accession
E-MTAB-12594:sc_E3_NPC
Submitter Id
41
year
age
ArrayExpress
broker name
hepatocyte
cell_type
portal vein embolization
clinical history
embolized tissue
clinical information
human
common name
Protocols: Human adult liver tissue samples were obtained from macroscopically healthy tissue that remained from resected human liver of patients with primary or secondary liver tumors or benign liver diseases with or without pretreatment with portal vein embolization. Informed consent of the patients for the use of tissue for research purposes was obtained according to the ethical guidelines of Leipzig University Hospital (006/17-ek, 21 March 2017, revised and renewed 12 February 2019). Acquired tissue from portal vein embolized livers included samples of regenerating (R) and embolized (E) tissue, whereas tissue samples from benign liver diseases were defined as quiescent healthy controls (H). Single stranded cDNA was generated in droplets according to 10X Genomics 3' Single cell Kit v2 and v3 guide instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. cDNA libraries were prepared as described in the 10X Genomics user guide (Single Cell 3′ Reagent Kits User Guide PN-120233, 10X Genomics). Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays
description
adult
developmental stage
normal
disease
individual 6
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
liver
organism part
non-parenchymal
sampling site
Homo sapiens
scientific_name
male
sex
sc_E3_NPC
title