2024-04-17
ENA first public
ERC000011
ENA-CHECKLIST
School of Cancer Sciences, University of Glasgow
INSDC center name
public
INSDC status
ERS18414408
SRA accession
E-MTAB-13897:Vg4_Ly6C+_1
Submitter Id
12 to 25
week
age
ArrayExpress
broker name
mature gamma-delta T cell
cell_type
not collected
collection date
house mouse
common name
Protocols: Cells were FACS sorted (DAPI-CD3+TCRD+CD27+, then Vg1+ or Vg4+ followed by gating on Ly6C positive or negative) from pooled lymph nodes (inguinal, brachial and axillary) and spleens of 10 wildytpe female C57BL/6 mice per biological replicate raw sequence quality was assessed using FastQC v. 0.11.8, trimmed using FastP, trimmed sequences aligned to mouse genome using STAR, raw counts per gene determined using FeatureCounts v.1.6.4 RNA isolation was performed using Qiagen RNeasy Mini Kit (74106) according to manufacturer's instructions SMARTer Stranded Total RNA-seq v2 - Pico Input Mammalian (Takara)
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
spleen and lymph nodes
organism part
Mus musculus
scientific_name
female
sex
C57BL/6
strain
Vg4_Ly6C+_1
title