2024-04-18
ENA first public
ERC000011
ENA-CHECKLIST
Fondazione Istituto Nazionale di Genetica Molecolare (INGM)
INSDC center name
public
INSDC status
ERS18894247
SRA accession
E-MTAB-13721_2:SQ_2719_PIC-0020_Exp_T0_CD4_TCR
Submitter Id
33
year
age
ArrayExpress
broker name
CD4+ T-lymphocyte
cell_type
SARS-CoV-2 Experienced
clinical history
not collected
collection date
human
common name
Protocols: Peripheral Blood Mononuclear Cells (PBMCs) were isolated by density-gradient centrifugation using Ficoll-Plaque protocol and cryopreserved for subsequent cell sorting and scRNA-Seq analyses.  Single cell suspension was used according to Chromium Next GEM Single cell V(D)J Reagent kits v1.1 Chromium Next GEM Single Cell 5’ Library & Gel Bead Kit v1.1, 16 rxns PN-1000165 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Single Index Kit T Set A, 96 rxns PN-1000213 Chromium Next GEM Single Cell 5’ Library & Gel Bead Kit v1.1, 16 rxns PN-1000165 Chromium Single Cell 5' Library Construction Kit, 16 rxns PN-1000020 Chromium Single Cell V(D)J Enrichment Kit, Human T Cell, 96 rxns PN-1000005 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Single Index Kit T Set A, 96 rxns PN-1000213  Chromium Next GEM Single Cell 5’ Library & Gel Bead Kit v1.1, 16 rxns PN-1000165 Chromium Single Cell 5' Library Construction Kit, 16 rxns PN-1000020 Chromium Single Cell V(D)J Enrichment Kit, Human B Cell, 96 rxns PN-1000016 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Single Index Kit T Set A, 96 rxns PN-1000213 Chromium Next GEM Single Cell 5’ Library & Gel Bead Kit v1.1, 16 rxns PN-1000165 Chromium Single Cell 5' Library Construction Kit, 16 rxns PN-1000020 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Single Cell 5' Feature Barcode Library Kit, 16 rxns PN-1000080 Single Index Kit N Set A, 96 rxns PN-1000212
description
adult
developmental stage
Healthy donor
disease
not collected
geographic location (country and/or sea)
PIC-0020
individual
not applicable
isolate
TCR Sequencing
library type
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
male
sex
SQ_2719_PIC-0020_Exp_T0_CD4_TCR
title