2024-04-18
ENA first public
ERC000011
ENA-CHECKLIST
Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium. Center for Plant Systems Biology, VIB, 9052 Gent, Belgium.
INSDC center name
public
INSDC status
ERS18602024
SRA accession
E-MTAB-13956:29_Col-0_R2
Submitter Id
10
day
age
ArrayExpress
broker name
not collected
collection date
thale cress
common name
Col-0
cultivar
Protocols: For RNA extraction,10-day-old plants grown horizontally on the round plates were used. Seedlings were immediatly frozen in liquid nitrogen. NA Young seedlings were grown horizontally on round plates containing ½ MS medium with 1% sucrose and 0.9% agar at 21˚C under a 16h/8h day/night condition. NA NA RNA was extracted with the miRNeasy Mini Kit (Qiagen). RNA integrity and quantity were measured using the Agilent 2100/4200 system.  The RNAs were first selected via enrichment of their poly(A) tail and then further fragmented into 300~350 bp fragments to build the libraries.
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Arabidopsis thaliana
organism
Arabidopsis thaliana
organism
whole seedling
organism part
Arabidopsis thaliana
scientific_name
29_Col-0_R2
title