2024-04-21
ENA first public
ERC000011
ENA-CHECKLIST
Southern University of Science and Technology
INSDC center name
public
INSDC status
ERS18352874
SRA accession
E-MTAB-13856:siTfeb_rep1
Submitter Id
0
day
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Dissection of VZs from neonatal mouse brains was performed as described 1. Briefly, the midbrain, the olfactory bulb, meninges and the hippocampus were removed in sequence to attain the telencephalon. Then VZ-containing tissues were separated from the telencephalon. Tissues from 4 mice were pooled, transferred to a 1.5ml Eppendorf tube, and digested with freshly prepared 10U/ml Papain solution (Worthington, catalog no. LS003126) at 37°C for 30 minutes. Then the Papain solution was aspirated, and 1ml culture medium (20% FBS) was added, followed by 1 minute of incubation to terminate the digestion. The supernatant was removed, and another 1ml culture medium (20% FBS) was added. The digested tissues were gently pipetted up and down by 20 times with a P1000 tip to dissociate the cells mechanically. Centrifuge at 350rcf for 5 minutes and discard the supernatant.  DNA was purified first by Qiagen minElute PCR purification kit according to the manufacturer's instruction, then by AmupureXP beads with 0.5X upper cutoff and a 1.2X lower cutoff according to manufacturer's instruction. Libraries were constructed in the Illumina Nextera DNA Library preparation kit.
description
EPC
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
ventricular zone
organism part
Mus musculus
scientific_name
siTfeb
stimulus
C57BL/6Jax
strain
siTfeb_rep1
title