2024-04-23
ENA first public
ERC000011
ENA-CHECKLIST
CNIC
INSDC center name
public
INSDC status
ERS18930729
SRA accession
E-MTAB-14010:FJD-15-11-22-WTA-Asmatico
Submitter Id
79
year
age
ArrayExpress
broker name
eosinophil
cell_type
not collected
collection date
human
common name
Protocols: Eosinophils were purified from the peripheral blood of healthy controls and asthmatic donors. For eosinophil purification, CD2, CD3, CD14, CD16, CD19, CD20, CD36, CD56, CD123, and glycophorin A positive cells were discarded by a magnetic bead separation technique, as described in the manufacturer's procedure (EasySep; StemCell Technologies, Vancouver, Canada). Eosinophils whole transcriptome analyses were performed using the BD Rhapsody™ Single Cell Analysis System (BD Biosciences). Purified eosinophils (120000 cells) were centrifuged and resuspended in 650 μL of BD sample buffer supplemented with 1:1000 SUPERase-In (20 U/μL; AM2694 ThermoFisher) and NxGen Rnase Inhibitors (40 U/μL; 30281-2 Lucigen), as previously described. For each sample, 20000-40000 cells were loaded into a different Rhapsody Single-Cell Analysis System cartridge. Cell capture and cDNA synthesis were performed according to manufacturer instructions.
description
adult
developmental stage
asthma
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
female
sex
FJD-15-11-22-WTA-Asmatico
title