2024-04-30
ENA first public
ERC000011
ENA-CHECKLIST
INSERM U1292
INSDC center name
public
INSDC status
ERS18960705
SRA accession
E-MTAB-14012:Sample 8
Submitter Id
25
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: 25-week-old mice were anesthetized using pentobarbital injection (180mk/kg) and successively perfused through the inferior veina cava with ethylene glycol tetraacetic acid buffer and collagenase II (Merck-Biochrom, Berlin, Germany). Livers were then harvested and cells were isolated using Histodenz (Sigma-Aldrich) gradients. Cell pellets were directly frozen at -80°C for bulk RNA sequencing experiments or used for RNA extraction.  All designed samples were treated using poly A+ RNA selection. mRNA extraction was conducted using truseq mRNA Illumina kit.
description
adult
developmental stage
BMP9-KO
genotype
not collected
geographic location (country and/or sea)
F133
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
liver
organism part
Mus musculus
scientific_name
female
sex
129/Ola
strain
Sample 8
title