2024-04-30
ENA first public
ERC000011
ENA-CHECKLIST
Vanderbilt University
INSDC center name
public
INSDC status
ERS18678158
SRA accession
E-MTAB-13964:Sample 2
Submitter Id
4
month
age
ArrayExpress
broker name
Cd11b+
cell_type
not collected
collection date
house mouse
common name
Protocols: Mice were perfused with heparinized (10U/ml) DPBS and a single cell suspension of the brain was prepared using the adult brain dissociation kit. Cd11b positive and negative cells were isolated via MACS with Cd11b-coated microbeads according to the manufacturer's instructions. After two washes with cell suspension buffer, cells were counted on a hemocytometer and diluted to target 2000 (Cd11b+) or 20,000 (Cd11b-) cells for encapsulation. Cells were encapsulated using the PIPseq method, v3 chemistry. The PIPseq manufacturer's guide was then followed for nucleic acid extraction. The PIPseq manufacturer's guide was then followed for library preparation.
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
brain
organism part
Mus musculus
scientific_name
male
sex
C57BL/6J
strain
Sample 2
title