2024-04-30
ENA first public
ERC000011
ENA-CHECKLIST
Kyung Hee University
INSDC center name
public
INSDC status
ERS18601493
SRA accession
E-MTAB-13943:Nipponbare_3
Submitter Id
ArrayExpress
broker name
not collected
collection date
Japanese rice
common name
Dongjin
cultivar
Protocols: Stages 13-14 anthers carrying tricellular stages pollen were collected. Seeds of japonica rice cv. Nipponbare were grown in the greenhouse for 4 weeks and transferred to the paddy field.  Mature anthers were frozen in LN2 and finely ground using Tissuelyser (QIAGEN). Then, total RNA was extracted using the MiniBEST Plant RNA Extraction Kit (Takakra, Japan) according to the manufacturer’s instructions. The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, \"tagmentation\" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified.
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Oryza sativa Japonica Group
organism
Oryza sativa Japonica Group
organism
anther
organism part
Oryza sativa Japonica Group
scientific_name
Nipponbare_3
title