2024-05-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA112655800
External Id
Forest Botany and Tree Physiology, University of Geottingen
INSDC center name
2023-02-17T12:33:29Z
INSDC last update
public
INSDC status
ERS14653351
SRA accession
E-MTAB-12654:S1-t4-C
Submitter Id
4
week
age
ArrayExpress
broker name
Populus x canescens
cultivar
Protocols: Per plant: harvest of full leaf (3,4,5) without petiole, pooling and freezing in liquid nitrogen Populus × canescens plants were in vitro propagated in glass jars (weck jars) according to Müller et al. 2013 Frontiers in Plant Science. Four week old Populus × canescens were co-cultivated with Laccaria bicolor (sandwich culture) according to Müller et al. 2013 Frontiers in Plant Science. The co-cultures were incubated for one, two, four and seven days until sample collection. The experiment was independently repeated in three times. Total RNA was extracted with innuPrep Plant RNA kit (Analytik Jena, Jena, Germany). The RNA of three samples was pooled. The library was constructed according to the manufacturer's protocol
description
LP.07 seven leaves visible stage
developmental stage
wild type genotype
genotype
none
growth condition
none
infect
not applicable
isolate
Populus tremula
organism
Populus tremula
organism
leaf
organism part
1
replicate
mixed leaf three four and five
sampling site
Populus tremula
scientific_name
S1-t4-C
title