2024-05-06
ENA first public
ERC000011
ENA-CHECKLIST
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo (HCFMUSP), Sao Paulo, Brazil
INSDC center name
public
INSDC status
ERS19126792
SRA accession
E-MTAB-14035:AIJ-12
Submitter Id
33
year
age
ArrayExpress
broker name
CD14-positive, CD16-negative classical monocyte
cell_type
not collected
collection date
human
common name
Protocols: Blood (70 ml) from RA patients and control group were collected, and peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density and dextran sedimentation. In both studied groups, monocytes were isolated from PBMCs by depletion of non-monocytes cells using an indirect magnetic labeling system. Classical monocytes were indirectly isolated using the CD16 MicroBeads human kit (Miltenyi Biotec®, Germany). Classical monocytes RNA was isolated using the RNeasy Plus Mini (Qiagen®), and RNA integrity number (RIN ≥ 7) was evaluated by the ScreenTape method using Bioanalyser 2100 (Agilent®). RNA library was constructed using the Quant-seq® 30 mRNA-Seq Library Prep Kit (Lexogen®).
description
adult
developmental stage
Polyarticular juvenile idiopathic arthritis
disease
not collected
geographic location (country and/or sea)
AIJ-12
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
female
sex
AIJ-12
title