2024-05-07
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113612133
External Id
EMBL
INSDC center name
2023-06-19T10:58:20Z
INSDC last update
public
INSDC status
ERS15606309
SRA accession
E-MTAB-13088:17209
Submitter Id
39
week
age
ArrayExpress
broker name
sperm
cell_type
not collected
collection date
house mouse
common name
Protocols: The cauda were then incubated in 1 ml of Donners solution (25mM NaHCO3, 1mM Sodium pyruvate, 0.53% Sodium lactate, 2% BSA) at 37°C with 20rpm shaking for 1hr after making 3-5 incisions in the cauda to allow for the sperm to swim out. After swim out the sperm solution was filtered through 40µm nylon mesh, washed in PBS and frozen in 200µl of Freezing media (Irvine Scientific Catalo#90128) at -80°C. Following ChIP, the washed antibody bound chromatin was eluted by heating the dynabead – antibody complex at 65°C at 400 rpm for 10 min in elution buffer (1X TE buffer, 5mM DTT, 100 mM NaHCO3, 0.2% SDS). The supernatant was transferred to DNA LoBind tubes, digested with RNase and Proteinase K and the DNA cleaned and concentrated using ChIP DNA cleanup and concentrator kit (Zymo research Catalog# - D5201) according to manufacturer instructions. 2.5 µg of ChIP DNA was used as starting material for each sample. The sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB Catalog# - E7645S) following manufacturer instructions.
description
adult
developmental stage
gene knock in
genetic modification
FVB/NCrl-Trim66em2(gfp)Emr
genotype
not collected
geographic location (country and/or sea)
17209
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
testes
organism part
male fertility
phenotype
Mus musculus
scientific_name
male
sex
FVB
strain
17209
title