2024-05-08
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114630232
External Id
University of Cambridge
INSDC center name
2023-11-16T14:34:32Z
INSDC last update
public
INSDC status
ERS17014231
SRA accession
E-MTAB-13539:AV2-WT_rep3_14d_small_RNA_seq
Submitter Id
3
week
age
ArrayExpress
broker name
not collected
collection date
Protocols: To prepare the samples leaves were cut from the plants and frozen in liquid nitrogen Plants were grown in a growth chamber in the following conditions: 22C, 16h light, 60% humidity For virus infection agrobacterium strains carrying pDIVA-AV2RNA1, pDIVA-AV2RNA2, pDIVA-AV2RNA3 (or its mutant version) and pDIVA-CP-RT were mixed and infiltrated into two leaves on each plant of 10 days old N. benthamiana. All agrobacterium strains were adjusted to a final optical density (OD600) of 0.2 RNA was extracted using Zymo research, Direct-zol RNA Miniprep plus kit For small RNA-seq 10mcg of low-molecular-mass RNA fraction, for Ribo-Seq ribosomal protected fragments, for RNA-seq hydrolysed RNA fragments, were separated by Urea-PAGE. RNA bands within the appropriate size range were acrylamide gel-purified. All library amplicons were constructed using a small RNA cloning strategy (Guo et al., 2010).
description
not collected
geographic location (country and/or sea)
Asparagus Virus 2 WT
infect
not applicable
isolate
Nicotiana benthamiana
organism
Nicotiana benthamiana
organism
leaf
organism part
Nicotiana benthamiana
scientific_name
AV2-WT_rep3_14d_small_RNA_seq
title